{"title":"Genome-Wide Identification and Characterization of the SBT Gene Family in maize and Its Expression in the Various tissues","authors":"Xiaodong Wang, Yanting Zhang, Dianjun Xiang, Meng Wang, Weiwei Zhang, Zhigang Li, Peng Liu","doi":"10.1007/s11105-023-01422-5","DOIUrl":"https://doi.org/10.1007/s11105-023-01422-5","url":null,"abstract":"<p>Maize is one of the three major food or feed crops in China. <i>Bacillus subtilis</i> protease like serine protease (SBT) is a serine proteolytic enzyme, which widely exists in various organisms and participates in biological growth and defense. However, SBT genes functions remained uncharacterized in growth and stress response in maize. Therefore, here we characterized the <i>SBT</i> gene family of maize. In this study, we identified 59 maize <i>SBT</i> genes (<i>ZmSBTs</i>), which were divided into 6 subfamilies through phylogenetic analysis. <i>ZmSBTs</i> are distributed unevenly on 10 chromosomes in Maize. As expected, <i>ZmSBTs</i> contained cis-acting elements for plant growth and defense. In addition, the transcriptome data of different maize tissues shows the dynamic differential expression of <i>ZmSBTs</i> in different tissues. We also predicted the interaction of <i>ZmSBT1.7</i> and <i>ZmSBT2.5</i> specifically expressed in meiosis_tassels with other maize proteins. We revealed that the <i>ZmSBT1.2</i> is specifically expressed in seeds with other maize proteins. This study provided valuable information about maize SBT gene family and clarified the possible biological functions of <i>ZmSBT1.7</i>, <i>ZmSBT2.5</i>, and <i>ZmSBT1.2</i>. The identified candidate genes could be used to breed higher-yielding Maize cultivars to feed the world population.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"27 28","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138494759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shivani Sharma, Dalwinder Singh, Riya Joon, Vishnu Shukla, Ajit Pal Singh, Palvinder Singh, Shrikant Mantri, Ajay K. Pandey
{"title":"System Analysis of Differentially Expressed miRNAs in Hexaploid Wheat Display Tissue-Specific Regulatory Role During Fe-Deficiency Response","authors":"Shivani Sharma, Dalwinder Singh, Riya Joon, Vishnu Shukla, Ajit Pal Singh, Palvinder Singh, Shrikant Mantri, Ajay K. Pandey","doi":"10.1007/s11105-023-01421-6","DOIUrl":"https://doi.org/10.1007/s11105-023-01421-6","url":null,"abstract":"<p>Iron (Fe) is an essential mineral element, and its deficiency in soil largely affects crop productivity. In plants, the molecular mechanisms underlying the genetic regulation of Fe-deficiency responses pinpointing microRNA (miRNA)-mediated regulation of Fe-deficiency response and its regulatory network are largely unaddressed. In the present study, we performed a small RNA-targeted whole-genome transcriptome analysis from hexaploid wheat and identified small RNAs (sRNAs) responding to Fe deficiency. Detailed analysis identified 105 differentially expressed miRNAs corresponding to Fe-deficiency response, and nine miRNAs were found to be novel in this study. Interestingly, tissue-specific regulation of Fe-deficiency response also participates through miRNA-mediated regulation. We identified 17 shoot-specific miRNAs and 18 root-specific miRNAs with altered expression. We validated the tissue specificity of these <i>miRNAs</i> by stem-loop quantitative RT-PCR that confirmed a temporal regulation. Furthermore, an attempt was made to predict their targets to speculate their participation in Fe-deficiency response. The miRNA target prediction analysis suggested a few major target genes, such as multicopper oxidases, E3 ubiquitin ligases, GRAS family, and WRKY transcription factors; those are previously known to play key roles in Fe homeostasis. The first preliminary information generated here will classify the repository of wheat <i>miRNAs</i> (with few novel miRNAs) for their role in Fe-deficiency response. Our work provides insights into miRNA-mediated regulatory pathways during Fe deficiency.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"27 29","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138494758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uncovering Alternate Splicing Events and Premature Stop Codons Associated with Early Sucrose Accumulation in Sugarcane Using BSR-seq","authors":"Nandita Banerjee, Sanjeev Kumar, Ayyadurai Anna Durai, Mamta Kumari, Sanjeev Kumar","doi":"10.1007/s11105-023-01418-1","DOIUrl":"https://doi.org/10.1007/s11105-023-01418-1","url":null,"abstract":"<p>Identification of the source of genetic variation in the large and complex genome of sugarcane has been comparatively less explored through next-generation sequencing tools. In this study, RNA-seq libraries of two extreme bulks from a segregating full-sib population and its parents were used to identify highly significant, alternate splicing (AS) events and premature stop codons (PMC) associated with early sucrose accumulation. An important differential AS event was identified within the β-subunit of pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP) which plays a key role in carbon partitioning in sugarcane. Premature stop codons that could result in truncated proteins were also detected in genes coding for aquaporin, aldolase, cytochrome C-oxidase, ribophorin and plant plasma membrane intrinsic proteins. A major proportion of these AS events and premature stop codons was validated for their differential enrichment in an independent set of sugarcane varieties having high and low sucrose content. The results provide useful insights into the role of putative AS events and premature stop codons which could be useful as candidate markers in marker-assisted breeding for developing high sugar varieties in sugarcane.</p>","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"27 30","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138494757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Abdur Rauf Sarkar, Salim Sarkar, Md Shohel Ul Islam, Fatema Tuz Zohra, Shaikh Mizanur Rahman
{"title":"Genome-Wide Identification and Characterization of LIM Gene Family in Grapevine (Vitis vinifera L.) and Their Expression Analysis at Early Bud Developmental Stages","authors":"Md. Abdur Rauf Sarkar, Salim Sarkar, Md Shohel Ul Islam, Fatema Tuz Zohra, Shaikh Mizanur Rahman","doi":"10.1007/s11105-023-01416-3","DOIUrl":"https://doi.org/10.1007/s11105-023-01416-3","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"2 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135041839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcones Ferreira Costa, Jonathan Andre Morales Marroquín, Carlos Eduardo de Araújo Batista, Alessandro Alves-Pereira, Fábio de Almeida Vieira, Maria Imaculada Zucchi
{"title":"Population Genomics and Conservation of Copernicia alba: a Non-Timber Palm of Economic Importance in the Brazilian Chaco","authors":"Marcones Ferreira Costa, Jonathan Andre Morales Marroquín, Carlos Eduardo de Araújo Batista, Alessandro Alves-Pereira, Fábio de Almeida Vieira, Maria Imaculada Zucchi","doi":"10.1007/s11105-023-01419-0","DOIUrl":"https://doi.org/10.1007/s11105-023-01419-0","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"106 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135137530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amar Jeet Gupta, Yogesh P. Khade, V. Mahajan, S. R. Hange, R. S. Shalaka, Major Singh
{"title":"Morphological and Molecular Characterization of Multiplier Onion (Allium cepa var. aggregatum) Genotypes","authors":"Amar Jeet Gupta, Yogesh P. Khade, V. Mahajan, S. R. Hange, R. S. Shalaka, Major Singh","doi":"10.1007/s11105-023-01415-4","DOIUrl":"https://doi.org/10.1007/s11105-023-01415-4","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"52 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135863869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadia Gul, Javaid Ashraf Nowshehri, Mudasir Ahmad Mir, Saima Wani, Javid I Mir, M. Ashraf Bhat
{"title":"MicroRNA: A Novel Micro-machineries to Target Crop Plants for Tolerance to Temperature Stress","authors":"Nadia Gul, Javaid Ashraf Nowshehri, Mudasir Ahmad Mir, Saima Wani, Javid I Mir, M. Ashraf Bhat","doi":"10.1007/s11105-023-01413-6","DOIUrl":"https://doi.org/10.1007/s11105-023-01413-6","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136013125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jônatas Chagas de Oliveira, André Lucas Domingos da Silva, Luciélio Manoel da Silva, Eduardo Fernandes Formighieri, Leila Priscila Peters, Giselle Mariano Lessa de Assis, Carla Cristina da Silva, Anete Pereira de Souza, Tatiana de Campos
{"title":"Novel Microsatellite Markers Derived from Arachis pintoi Transcriptome Sequencing for Cross-Species Transferability and Varietal Identification","authors":"Jônatas Chagas de Oliveira, André Lucas Domingos da Silva, Luciélio Manoel da Silva, Eduardo Fernandes Formighieri, Leila Priscila Peters, Giselle Mariano Lessa de Assis, Carla Cristina da Silva, Anete Pereira de Souza, Tatiana de Campos","doi":"10.1007/s11105-023-01402-9","DOIUrl":"https://doi.org/10.1007/s11105-023-01402-9","url":null,"abstract":"","PeriodicalId":20215,"journal":{"name":"Plant Molecular Biology Reporter","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136293911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}