The Transcription Factor Gene RDD3 Improves Ammonium and Magnesium Uptake and Accumulation and Drought Tolerance in Rice

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-12-08 DOI:10.1007/s11105-023-01428-z

Masao Iwamoto

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Abstract

We investigated the expression and functions of a transcription factor gene, RDD3. It displays sequence similarity to RDD1, which controls various nutrient ion accumulation and uptake in rice. The RDD3 protein was specifically localized to protoxylems and metaxylems in the vascular bundles. RDD3-overexpressing (RDD3-OX) plants increased the RDD3 protein levels early in the light period and improved NH₄⁺ and Mg²⁺ uptake and accumulation in the shoots. Furthermore, photosynthetic CO₂ assimilation and stomatal conductance in RDD3-OX plants were higher than in wild-type plants, although shoot dry weight was decreased in mature RDD3-OX plants. Subsequent microarray analysis indicated that the late embryogenesis abundant (LEA) protein genes associated with drought responses were upregulated in RDD3-OX plants, whereas WRKY transcription factor genes were downregulated despite participating in defenses against biotic and abiotic stresses. Examining drought stress tolerance indicated that RDD3-OX plants were more tolerant than wild-type plants. These results indicate that increased RDD3 protein levels early in the light period improve nitrogen and magnesium accumulation and drought stress tolerance in RDD3-OX plants.

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转录因子基因 RDD3 可提高水稻对铵和镁的吸收和积累以及抗旱能力

我们研究了转录因子基因 RDD3 的表达和功能。它与 RDD1 的序列相似，后者控制着水稻中各种营养离子的积累和吸收。RDD3 蛋白特异性定位于维管束中的原木质部和偏木质部。RDD3过表达（RDD3-OX）植株在光照初期提高了RDD3蛋白水平，并改善了芽中NH4+和Mg2+的吸收和积累。此外，RDD3-OX 植株的光合 CO2 同化和气孔导度均高于野生型植株，但 RDD3-OX 成熟植株的芽干重有所下降。随后的芯片分析表明，在 RDD3-OX 植株中，与干旱响应相关的胚胎发生后期丰富蛋白（LEA）基因上调，而 WRKY 转录因子基因下调，尽管它们参与了对生物和非生物胁迫的防御。对干旱胁迫耐受性的研究表明，RDD3-OX 植物比野生型植物更耐旱。这些结果表明，在光照初期增加 RDD3 蛋白水平可提高 RDD3-OX 植物的氮和镁积累以及干旱胁迫耐受性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.