Chromosomal Positions of 5S and 45S rDNA in Some Iranian Fritillaria (Liliaceae) Species

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-09 DOI:10.1007/s11105-024-01467-0

Neda Seifoori, Ghader Mirzaghaderi, Shahla Hosseini

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Abstract

The present investigation systematically examined the distribution of 5S and 45S rDNA sites in six distinct Fritillaria species, all characterized as diploid with 2n = 2x = 24 chromosomes. Fritillaria assyriaca ecotypes displayed variable numbers of B chromosomes (Bs), ranging from one to four, while F. zagrica exhibited two additional B chromosomes. Terminal or sub-terminal chromosomal regions harbored one to two pairs of 5S rDNA sites. Regarding 45S rDNA sites, F. raddeana featured a singular pair, F. assyriaca, F. zagrica, and F. persica exhibited three pairs, F. avromanica presented four pairs, and F. chlorantha displayed eight pairs, predominantly situated distally, except for F. raddeana and F. chlorantha, which demonstrated interstitial and sub-terminal locations, respectively. Chromosome and karyotype indices facilitated the identification of, F. avromanica and F. chlorantha as species with the most symmetrical and asymmetrical chromosomes, respectively. Cluster analysis of the karyotype similarity matrix revealed incongruities between the observed number and distribution of rDNA sites and the established taxonomic classifications, particularly notable in F. chlorantha within the Fritillaria subgenus. The results provide significant insights into the genetic diversity and karyotype characteristics of Fritillaria, challenging conventional taxonomic frameworks. The observed variations in the numbers and locations of rDNA sites underscore the necessity for a nuanced understanding of genetic relationships within the Fritillaria subgenus.

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伊朗一些凤仙花（百合科）物种的 5S 和 45S rDNA 染色体位置

本研究系统地考察了六种不同鱼腥草中 5S 和 45S rDNA 位点的分布，这些鱼腥草均为二倍体，具有 2n = 2x = 24 条染色体。Fritillaria assyriaca 生态型的 B 染色体（Bs）数目不等，从 1 条到 4 条不等，而 F. zagrica 则有两条额外的 B 染色体。染色体末端或亚末端区域含有一到两对 5S rDNA 位点。关于 45S rDNA 位点，F. raddeana 只有一对，F. assyriaca、F. zagrica 和 F. persica 有三对，F. avromanica 有四对，F. chlorantha 有八对，主要位于远端，只有 F. raddeana 和 F. chlorantha 分别位于间隙和亚末端。染色体和核型指数有助于确定 F. avromanica 和 F. chlorantha 分别是染色体最对称和最不对称的物种。核型相似性矩阵的聚类分析显示，观察到的 rDNA 位点的数量和分布与已确立的分类学分类之间存在不一致之处，尤其是在 Fritillaria 亚属中的 F. chlorantha。这些结果为了解 Fritillaria 的遗传多样性和核型特征提供了重要信息，对传统的分类框架提出了挑战。观察到的 rDNA 位点数量和位置的变化突出表明，有必要细致地了解 Fritillaria 亚属内部的遗传关系。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.