Plant Cell Reports最新文献

{"title":"Cinnamyl alcohol acyltransferase PmCAAT1 specifically catalyzes the formation of the characteristic volatile cinnamyl acetate of Prunus mume.","authors":"Jinhong Li, Jialu Guan, Shengwei Zhang, Meng Lei, Jiahui Li, Yanlong Zhang, Tengxun Zhang","doi":"10.1007/s00299-026-03992-6","DOIUrl":"10.1007/s00299-026-03992-6","url":null,"abstract":"<p><strong>Key message: </strong>Three cinnamyl alcohol acyltransferases (PmCAAT1-3) were identified in Prunus mume, with PmCAAT1 showing high substrate preference for cinnamyl alcohol and PmCAAT2 exhibiting broader substrate specificity including linalool. Prunus mume is renowned for its distinctive fragrance, with cinnamyl acetate serving as one of its key aroma components. However, the genes responsible for cinnamyl acetate biosynthesis have remained unidentified. In this study, a total of 116 acyltransferase family members were identified from the whole-genome data of P. mume var. tortuosa. Among these, three cinnamyl alcohol acyltransferase genes (PmCAAT1-3) were selected through transcriptome data of different cultivars and RT-qPCR analysis of petals at four developmental flowering stages. Then PmCAAT1-3 were cloned. Sequence alignment revealed that their amino acid sequences contain the conserved HXXXD catalytic motif and DFGWG substrate-binding motif of the BAHD family. Phylogenetic analysis showed that PmCAAT1 is evolutionarily closer to the CFAT clade, whereas PmCAAT2 and PmCAAT3 clustered together on a separate branch. Subcellular localization indicated that PmCAAT1-3 are localized in the nucleus and cytoplasm. Transient expression in P. mume petals demonstrated that all three genes could regulate cinnamyl acetate biosynthesis. Prokaryotic expression and In vitro enzyme activity assays confirmed that PmCAAT1 and PmCAAT2 could catalyze the conversion of cinnamyl alcohol to cinnamyl acetate. Kinetic analysis revealed that although both PmCAAT1 and PmCAAT2 display broad substrate selectivity, PmCAAT1 exhibits a strong preference for cinnamyl alcohol. This study elucidates the enzymatic basis for cinnamyl acetate biosynthesis in P. mume and offers a theoretical foundation for molecular breeding of floral fragrance in this species.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149064034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of the mango YUCCA family and overexpression of MiYUCCA10A/B confers early flowering and stress tolerance in transgenic Arabidopsis and tomato.","authors":"Zhixi He, Wanli Hu, Liufei Qin, Chuting Huang, Ruoyan Li, Fang Xu, Fangfang Xie, Cong Luo, Xinhua He","doi":"10.1007/s00299-026-03981-9","DOIUrl":"10.1007/s00299-026-03981-9","url":null,"abstract":"<p><p>Auxin is a fundamental phytohormone that orchestrates developmental processes in plants. Flavin monooxygenase (YUCCA) serves as the rate-limiting enzyme in the indole-3-pyruvic acid pathway (IPA) pathway, and its main function is to convert IPA into IAA. In this study, 12 members of the MiYUCCA gene family were mined and identified from the genomic data of mango (Mangifera indica). Phylogenetic tree analysis classified mango YUCCA into five categories. Cis-elements analysis of the promoter indicated that the promoter region contained a large number of hormone response elements and abiotic elements. Expression analysis revealed that the majority of MiYUCCA gene family members displayed high expression abundance at the early developmental stages of fruits, and their expression decreased or even remained unexpressed with the fruit growth and development. MiYUCCA10A and MiYUCCA10B were selected for functional verification. Overexpression of MiYUCCA10A and MiYUCCA10B promoted the early flowering in transgenic Arabidopsis and tomato, enhanced the salt and drought resistance, and also increased the content of IAA in transgenic plants. In addition, MiYUCCA10A and MiYUCCA10B significantly reduced the seed number of transgenic Arabidopsis and tomato plants, exerted no effect on the pod length of Arabidopsis, but markedly decreased the fruit size of tomato. The interaction of MiYUCCA10A/B with the SHORT VEGETATIVE PHASE 1/2/3/4/5 (MiSVP1/2/3/4/5) and FLOWERING LOCUS C (MiFLC) was revealed using both yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses. These results indicate that the MiYUCCA10A/B were not only involved in flowering, but also related to stress tolerance and fruit development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149040388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome doubling is associated with enhanced self-compatibility and transcriptional remodeling of pollen-pistil interactions in Lycium chinense.","authors":"Zixuan Wang, Yingzhi Qin, Ken Qin, Bo Zhang, Jinhuan Chen","doi":"10.1007/s00299-026-03991-7","DOIUrl":"10.1007/s00299-026-03991-7","url":null,"abstract":"<p><strong>Key message: </strong>Cytology and 72-sample pistil transcriptomes link chromosome doubling to self-compatibility in autotetraploid Lycium chinense through altered pollen-pistil responses and candidate S-RNase trafficking and detoxification pathways. Polyploidy can reshape plant reproductive traits and self-compatibility systems, yet the mechanisms underlying ploidy-associated transitions to self-compatibility remain poorly understood. In this study, diploid and autotetraploid Lycium chinense were compared using floral morphology, stigma receptivity, pollen viability, and pollen tube cytology, controlled pollination, seed vigor analysis, and transcriptome sequencing of 72 pistil samples spanning stigma, style, and ovary tissues at four pollination stages. Chromosome doubling enlarged reproductive organs, prolonged stigma receptivity, and increased pollen size, while also altering pollen performance and pollen tube growth. Diploid self-pollen tubes were arrested in the upper style, whereas autotetraploid self-pollen tubes continued through the style, accompanied by a marked increase in self-compatibility, with the compatibility index rising from 0.50 to 5.70 and fruit set from 8.33% to 73.33%. Spatiotemporal transcriptome analysis revealed extensive tissue- and time-dependent transcriptional remodeling between ploidy levels. Temporal clustering and ploidy × time interaction analyses showed particularly pronounced divergence in the stigma and style, with the style exhibiting the strongest interaction response. WGCNA further identified tissue-associated co-expression modules enriched in membrane trafficking, phosphatidylinositol signaling, endocytosis, SNARE-mediated vesicular transport, and ubiquitin-mediated protein turnover. Reported GSI-related homologs, including LcS-RNase, LcHT-B, Lc120K, LcStEP, LcSBP1, and LcSKP1, also displayed distinct spatiotemporal expression patterns. Integrating these observations, we propose a working model in which heteroallelic pollen effects, altered S-RNase compartmentalization, and protein turnover may jointly reduce S-RNase-mediated cytotoxicity and permit continued self-pollen tube growth in autotetraploid L. chinense. These findings provide a cytological and transcriptomic framework for understanding ploidy-associated GSI breakdown and its potential relevance to polyploid breeding in Lycium.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149031189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics of sulphur mediated alleviation of lead toxicity in Brassica juncea.","authors":"Hemanthkumar Manne, Nisha Kumari, Sonia Nain, Kodidhala Vaishnavi, Abbu Zaid","doi":"10.1007/s00299-026-03990-8","DOIUrl":"10.1007/s00299-026-03990-8","url":null,"abstract":"<p><strong>Key message: </strong>Sulphur amelioration effectively mitigated lead toxicity in Brassica juncea L. by reducing oxidative stress and enhanced antioxidant defense, photosynthesis by detoxifying the lead accumulation. The current study was conducted to study physiological, yield and redox regulation under exogenous effect of sulphur (S1; 100 ppm, S2: 200 ppm) in lead stressed (Pb1; 100 ppm, Pb2; 200 ppm, Pb3; 300 ppm) Brassica juncea L. (RH 749) at 30, 60, 90 days after sowing (DAS). Lead stress of 300 ppm increased malondialdehyde (MDA) by 30.93, 32.73, 34.82% and electrolyte leakage (EL) 204, 116, 94.3% at 30, 60, 90 DAS, respectively. Moreover Pb3 stress increased ascorbic acid (AsA), glutathione (GSH) and phytochelatins by 53.1, 27.96, 362.73% at 90 DAS, respectively. Lead stress decreased yield and physiological parameters, such as transpiration rate, photosynthetic rate and stomatal conductance, by 33.6, 20.76, 33.36%, respectively, at 90 DAS. Besides, S2 spray to Pb3-stressed plants decreased MDA 13.49 and EL by 18.62%, respectively, at 90 DAS. Exogenous sulphur treatment further steadied the cellular homeostasis by increasing transpiration, photosynthesis, oil content and yield. Under Pb stress sulphur assimilating enzymes ATPS and GST increased. As a result of S assimilation in plants AsA, GSH and phytochelatins synthesis also increased and detoxified the toxic Pb. Besides, transcriptomic studies of BjATPS increased under Pb stress and S spray almost doubled fold expression and provided resistance to the plant. The present study concludes that S application alleviates Pb stress in Brassica seedlings by regulating oxidative biomarkers and antioxidants thus improving yield constraints and suggests that S could be a suitable choice for farmers in mitigating Pb toxicity in Brassica, where S not only employs in defense mechanisms against metal stress, but also regulates plant growth and development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homoeologous exchange-associated ABA catabolism rewiring contributes to salinity tolerance in a synthetic tetraploid rice.","authors":"Bingqi Zhang, Tiantian Zhu, Guo Li, Shu Ma, Di An, Changyin Cui, Yingying Tan, Chunming Xu, Ying Wu, Bao Liu","doi":"10.1007/s00299-026-03987-3","DOIUrl":"10.1007/s00299-026-03987-3","url":null,"abstract":"<p><strong>Key message: </strong>Altered ABA-related regulation is associated with transgressive salinity tolerance in a synthetic tetraploid rice carrying extensive post-polyploidy homoeologous exchanges. Plant polyploids often show higher environment resilience than their congeneric diploid progenitors. However, it remains unclear whether these traits arise directly from whole-genome duplication (WGD) or evolve through post-WGD changes. Here, we show that transgressive salt tolerance occurs in ca. 1.71% of an early-generation synthetic rice tetraploid from japonica-indica subspecies hybridization. Genome resequencing unravels extensive homoeologous exchanges (HEs) but without genomic features distinguishing the tolerant vs. sensitive plants. In contrast, RNA-seq-based transcriptome-profiling enables distinct separation of the tolerant and sensitive plants. A KEGG pathway analysis indicates that the downregulated genes in tolerant plants are enriched in the abscisic acid (ABA)-degradation pathway. Expression and coding-sequence analyses identified the ABA catabolic gene OsABA8ox3 as a candidate associated with the salt-tolerant phenotype. Endogenous ABA measurements and exogenous ABA and Na₂WO₄ treatments in selected tetraploid recombinant inbred lines further supported an association between ABA homeostasis and salinity tolerance. Our results suggest post-WGD regulatory diversification contributes to adaptive phenotypic variation in polyploids.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsbHLH6 regulates OsMADS57 and amino acid metabolism to impair cold tolerance in rice.","authors":"Jingyao Yu, Kang Li, Yu Yang, Yuhui Wang, Ling Liu, Yan Cheng, Luis Alejandro José Mur, Chuanying Fang","doi":"10.1007/s00299-026-03997-1","DOIUrl":"10.1007/s00299-026-03997-1","url":null,"abstract":"<p><strong>Key message: </strong>OsbHLH6 mediates a CBF-independent cold response through transcriptional repression and metabolic disruption. Cold stress severely impairs rice (Oryza sativa L.) productivity, yet the regulatory mechanisms underlying cold sensitivity remain incompletely understood. Here, we identify OsbHLH6 as a negative regulator of rice cold tolerance that acts through transcriptional repression and metabolic reprogramming. Transcriptomic profiling of OsbHLH6-overexpressing (OsbHLH6_OE) plants revealed suppression of key cold-resilience genes, including OsMADS57, a known positive regulator of cold adaptation. Yeast one-hybrid and electrophoretic mobility shift assays confirmed direct binding of OsbHLH6 to E-box motifs in the OsMADS57 promoter, repressing its transcription and downstream OsWRKY94 expression. Metabolomic analysis under cold stress demonstrated that OsbHLH6_OE plants accumulated excessive arginine (Arg) and histidine (His), exacerbating reactive oxygen species (ROS) accumulation and membrane damage. Exogenous application of Arg and His exacerbated cold sensitivity in OsbHLH6_OE seedlings, linking amino acid dysregulation to impaired cold tolerance. Haplotype analysis revealed distinct OsbHLH6 alleles in japonica and indica subspecies, which originated from wild progenitors and were conserved during domestication. Our findings reveal that cold-induced OsbHLH6 transcription suppresses OsMADS57 and disrupts amino acid metabolism, each contributing to impaired cold tolerance. This study provides novel insights into bHLH-mediated stress adaptation and positions OsbHLH6 as a target for breeding cold-tolerant rice varieties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles released by in vitro plant cell cultures: emerging systems for vesicle-mediated communication and biomedical translation.","authors":"Francesca Cristiana Piritore, Arianna Minoia, João Pessoa, Maria Grazia Romanelli, Luca Dalle Carbonare, Maria Teresa Valenti","doi":"10.1007/s00299-026-03983-7","DOIUrl":"10.1007/s00299-026-03983-7","url":null,"abstract":"<p><strong>Key message: </strong>This article summarizes how extracellular vesicles are emerging as important means through which cells communicate with each other in plants and their potential medical applications. Extracellular vesicles are emerging as important mediators of intercellular communication in plants, with roles in defense responses, stress adaptation, and cross-kingdom molecular signaling. Although plant-derived extracellular vesicles have attracted increasing interest, most studies have focused on vesicles isolated from whole tissues or edible plant materials, whereas extracellular vesicles released by in vitro plant callus and suspension cell cultures remain comparatively underexplored. This review discusses plant cell cultures as controllable and potentially scalable experimental systems for investigating extracellular vesicle biogenesis, molecular cargo, isolation, characterization, and biological activity. Particular attention is given to the distinction between bona fide extracellular vesicles secreted by living plant cells and heterogeneous nanovesicle preparations generated through tissue disruption, a key issue for reproducibility and interpretation in plant EV research. We also summarize current evidence on vesicle-mediated communication, cargo modulation, engineering strategies, and potential biomedical applications. Finally, we highlight major methodological gaps, including the lack of plant-specific markers, standardized isolation workflows, quality control criteria, and harmonized reporting practices. By integrating plant cell biology, extracellular vesicle research, and plant biotechnology, in vitro plant cell cultures may provide useful model systems for studying vesicle-mediated communication and for developing biologically produced vesicles with more defined functional properties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13593712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative physiological, hormonal, and transcriptional analyses reveal the difference between diploid and autotetraploid rice under cadmium stress.","authors":"Wei Cai, Wen-Shu Wang, Hui Deng, Bin Chen, Guo Zhang, Ping Wang, Yong-Sheng Zhu","doi":"10.1007/s00299-026-03988-2","DOIUrl":"10.1007/s00299-026-03988-2","url":null,"abstract":"<p><strong>Key message: </strong>Elevated cadmium (Cd) stress tolerance in autotetraploid rice at the seedling stage is correlated with coordinated remodeling of ROS homeostasis, hormone signaling and transcriptional programs. Polyploid plants commonly exhibit robust tolerance to various abiotic stresses, while the underlying regulatory mechanisms remain largely elusive. To elucidate the intrinsic mechanisms underlying enhanced Cd stress tolerance in autotetraploid rice, two pairs of diploid (2X) rice and their corresponding autotetraploid (4X) lines were used as experimental materials for Cd stress treatment and systematic phenotypic, physiological, and molecular evaluation at the seedling stage. Phenotypic observations confirmed that autotetraploid rice displayed markedly stronger Cd stress tolerance than diploid rice, as reflected by approximately 1.5-fold greater plant height alongside elevated biomass and chlorophyll content. Physiological and biochemical assays revealed that autotetraploids exhibited markedly lower Cd content, higher levels of soluble sugars and proline, distinctly lower levels of ROS-related indicators (MDA and H₂O₂), and markedly elevated activities of antioxidant enzymes (catalase, CAT; peroxidase, POD). Comparative phytohormone quantification demonstrated that autotetraploid rice had significantly higher levels of tryptamine (TAM), indole-3-acetic acid (IAA), indole-3-acetyl-L-aspartic acid (IAA-Asp), jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and gibberellins A₁ (GA₁) and A₇ (GA₇) but significantly lower levels of cis- (cZ) and trans-zeatin (tZ), with about 2.6, 1.3, 2.3, 1.4, 2.0, 2.6, 2.1, 0.5, 0.6 folds of diploid plants, respectively. Transcriptome profiling revealed that under control conditions, the number of differentially expressed genes (DEGs) between autotetraploid and diploid was merely 939 in roots and 313 in shoots. Under Cd stress, however, the counts of DEGs markedly increased to 4974 in roots and 1023 in shoots. Notably, genes involved in heavy metal transport, and hormone and ROS metabolism were significantly enriched in autotetraploids. Furthermore, qRT-PCR validated consistent expression trends of core metal transporters, hormone biosynthesis genes, and antioxidant genes between the RNA-seq data and independent samples. Collectively, this study suggests that the distinct regulations of physiological and biochemical processes, hormone metabolism, and transcription in autotetraploids under Cd stress are correlated with their enhanced Cd stress tolerance.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tomato DOR encodes a signal peptide peptidase-like protein required for adventitious organogenesis and rooting.","authors":"Marybel Jáquez-Gutiérrez, Sandra Bretones, Constanza Martin-Vásquez, Rocío Fonseca, Alberto Aguiar, Benito Pineda, Rafael Lozano, Vicente Moreno, Fernando J Yuste-Lisbona, Alejandro Atarés","doi":"10.1007/s00299-026-03979-3","DOIUrl":"10.1007/s00299-026-03979-3","url":null,"abstract":"<p><strong>Key message: </strong>The identification of the tomato DOR gene that is essential for adventitious organogenesis provides a genetic target for improving regeneration efficiency and root system performance in tissue culture and plant breeding. Plant rooting and adventitious organogenesis represent major bottlenecks in the application of plant tissue culture techniques. In this study, we characterize the dor mutant (defective in organogenesis and rooting) identified in our collection of tomato T-DNA lines. This mutant exhibits normal callus proliferation but fails to differentiate adventitious buds. Additionally, it displays underdeveloped embryonic roots, and its adventitious roots derived from various explants are also altered. Grafting experiments revealed compromised in vivo development primarily due to its abnormal root system. Upon identifying an allelic mutant in another tomato line, we observed no co-segregation between a T-DNA insert and the phenotype in either of the identified allelic mutants. Through mapping-by-sequencing, we identified Solyc12g098670 as the gene responsible for this mutation, which is homologous to SIGNAL PEPTIDE PEPTIDASE-LIKE (SPPL) genes from Arabidopsis thaliana, particularly SPPL3 and SPPL5. The expression pattern of DOR in tomato is nearly ubiquitous, similar to SPPL3 in Arabidopsis, yet the regeneration and rooting of sppl3 Arabidopsis mutants resemble the wild-type. In both tomato and Solanum pennellii (Correll) D'Arcy, RNAi lines and plants edited by CRISPR/Cas exhibit a dor mutant phenotype. However, by increasing the expression level of DOR, the plants become indistinguishable from the wild-type in terms of in vitro and in vivo development. Overexpression of this gene in the mutant has enabled the regeneration of plants with a wild-type phenotype. These results demonstrate that DOR is the first member of the tomato SPPL gene family known to be associated with root development and adventitious organogenesis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13588877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RPT2a-mediated degradation of WDR5a regulates flowering time in Arabidopsis.","authors":"Jia Liu, Yang-Yang Liu, Jing Wu, Ya-Ting Zhang, Fangfang Yang, Qian Du, Li-Yu Chen","doi":"10.1007/s00299-026-03966-8","DOIUrl":"10.1007/s00299-026-03966-8","url":null,"abstract":"<p><strong>Key message: </strong>RPT2a and CUL4-DDB1A-mediated degradation of WDR5a regulates COMPASS-like complex-dependent H3K4me3 homeostasis and flowering time in Arabidopsis through the FLC-FT/SOC1 regulatory module. The Arabidopsis complex of proteins associated with Set1 (COMPASS)-like complex plays a crucial role in regulating H3K4me3, a key histone modification associated with active transcription. Within this complex, WD40-REPEAT 5a (WDR5a) functions as a structural backbone protein, critical for maintaining proper H3K4me3 levels. WDR5a is subject to degradation via the 26S proteasome pathway, a process that significantly influences H3K4me3 dynamics. Utilizing a yeast two-hybrid screen, we identified the 26S proteasome subunit REGULATORY PARTICLE AAA-ATPASE2a (RPT2a) as an interacting partner of WDR5a, highlighting its role in WDR5a's proteasomal degradation. Further investigation revealed that the ubiquitin ligase complex Cullin4 (CUL4)-DNA Damage Binding protein 1A (DDB1A) targets WDR5a for ubiquitination, with lysine residues K31 and K211 being potential ubiquitination sites. RPT2a's involvement in WDR5a degradation consequently affects the expression of the floral repressor FLOWERING LOCUS C (FLC), thereby establishing an inverse relationship with the expression of flowering time control genes FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1). As these genes are directly repressed by FLC, their modulation via WDR5a degradation ultimately influences flowering time in Arabidopsis, underscoring the complex interplay between histone modification and plant development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}