Plant Cell Reports最新文献

{"title":"Revisiting development and physiology of wild rice relatives for crop improvement and climate resilience.","authors":"Jyotirmaya Mathan, Aditi Dwivedi, Aashish Ranjan","doi":"10.1007/s00299-025-03448-3","DOIUrl":"10.1007/s00299-025-03448-3","url":null,"abstract":"<p><strong>Key message: </strong>The review summarizes developmental and physiologic traits of wild rice relatives that can be targeted in mainstream rice-improvement programs for yield increases under changing climate. Increasing rice yield and productivity under changing climatic conditions is imperative for sustainable food security, given rice is a major staple crop around the world. Natural variation in crop plants, including wild relatives, offers remarkable genetic variability to explore the desirable developmental and physiologic traits for crop improvement. Wild relatives of rice, with distinct developmental and physiologic features compared to cultivated varieties, are the potential genetic and genomic resource for rice yield increases under changing climate. A thorough genetic basis of rice developmental and architectural changes during domestication is now established with the identification and characterization of domestication genes. Photosynthetically efficient wild rice accessions, with desirable developmental, physiologic, and metabolic traits, have been identified in recent years that could be instrumental for rice improvement. While several abiotic and biotic stress-tolerant wild relatives of rice along with the associated genetic loci have been identified over the years, a comprehensive insight into the desirable developmental and physiologic attributes of the wild rice is limited. Moreover, the usage of wild rice is not streamlined in rice-improvement programs due to genetic and genomic constraints. In this review, we summarize the desirable developmental and physiologic features of wild rice species that can be exploited for combining yield increases with climate resilience in rice-improvement programs.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"55"},"PeriodicalIF":5.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic insights into fruit-pathogen interactions: managing biotic stress in fruit.","authors":"Aidhya Irhash Putra, Muhammad Naveed Khan, Nurhaida Kamaruddin, Raja Farhana R Khairuddin, Jameel R Al-Obaidi, Brenda Juana Flores, Luis Fernando Flores","doi":"10.1007/s00299-025-03443-8","DOIUrl":"10.1007/s00299-025-03443-8","url":null,"abstract":"<p><strong>Keymessage: </strong>Proteomics has revealed complex immune responses in fruits, leading to the identification of potential disease biomarkers and resistance mechanisms. Fruit diseases caused by fungal and bacterial pathogens present critical challenges to global food security by reducing fruit shelf life and quality. This review explores the molecular dynamics of fruit-pathogen interactions using advanced proteomic techniques. These approaches include mass spectrometry-based identification, gel-based, and gel-free strategies, tailored to the unique compositions of fruit tissues for accurate protein extraction and identification. Proteomic studies reveal pathogen-induced changes in fruit proteomes, including the upregulation of defence-related proteins and suppression of metabolic pathways crucial for pathogen survival. Case studies on tomatoes, apples, and bananas highlight specific pathogen-responsive proteins, such as PR proteins and enzymes involved in ROS scavenging, which play roles in disease resistance mechanisms. The review further demonstrates the utility of proteomic data in identifying early disease biomarkers, guiding genetic improvements for disease resistance, and optimizing pathogen control measures. Integrating proteomic insights with transcriptomics and metabolomics provides a multidimensional understanding of fruit-pathogen interactions, paving the way for innovative solutions in agriculture. Future research should prioritize multi-omics approaches and field-level validations to translate laboratory findings into practical applications. The advancements discussed underscore the transformative role of proteomics in improving food security and sustainability amid challenges posed by climate change and increasing global food demand.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"54"},"PeriodicalIF":5.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143409919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High concentration of phosphate treatment increased the tolerance of Robinia pseudoacacia roots to salt stress.","authors":"Honghao Gan, Jianmin Chu, Jia Sun, Qian Wang","doi":"10.1007/s00299-025-03446-5","DOIUrl":"10.1007/s00299-025-03446-5","url":null,"abstract":"<p><strong>Key message: </strong>High P increased the tolerance of R. pseudoacacia roots to salt stress. Salt is an important abiotic factor that restricts plant growth and development in soil. An appropriate concentration of P can increase plant tolerance to salt stress. We investigated the physiological and transcriptional regulatory effects of high P (HP) or low P (LP) on the response of R. pseudoacacia roots to salt stress. A pot experiment was carried out to grow R. pseudoacacia seedlings in vermiculite media supplemented with 0 mM, 150 mM or 300 mM NaCl under HP or LP conditions. The root dry weight and concentrations of free proline, P, ions, and phytohormones were measured, and the transcription of the genes was analyzed under NaCl stress under HP or LP conditions. The results revealed that R. pseudoacacia responds to NaCl stress by regulating the absorption and utilization of P and the levels of free proline, phytohormones and Na⁺, K⁺, Ca²⁺, and Mg²⁺ as well as changing the expression levels of key genes. Compared with those under the LP condition, the roots of the R. pseudoacacia under the HP condition presented greater P concentrations, lower JA concentrations, and more stable K⁺ levels when subjected to NaCl stress, which increased their tolerance to NaCl stress. Moreover, genes involved in the cell wall, root growth, root architecture regulation, biomass accumulation, stress response, osmotic regulation and ion balance maintenance were upregulated under NaCl stress under HP conditions. In addition, NaCl stress impairs N metabolism under LP conditions. Our findings provide new insights into the response of woody plants to salt stress under different P conditions and contribute to the development of scientific afforestation in saline-alkali areas.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"53"},"PeriodicalIF":5.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and expression analysis of the RBOH gene family of Isatis indigotica Fort. and the potential regulation mechanism of RBOH gene on H₂O₂ under salt stress.","authors":"Junbai Ma, Weichao Ren, Shan Jiang, Lingyang Kong, Lengleng Ma, Jiajun He, Danli Wang, Weili Liu, Wei Ma, Xiubo Liu","doi":"10.1007/s00299-025-03442-9","DOIUrl":"10.1007/s00299-025-03442-9","url":null,"abstract":"<p><strong>Key message: </strong>RBOH gene may regulate the resistance of Isatis indigotica Fort. to salt stress by mediating the production of H₂O₂. RBOH gene plays an important role in plant growth and development, abiotic and biotic stress response, and hormone signalling. However, studies on RBOH gene expression and molecular mechanism of Isatis indigotica Fort. under salt stress have not been reported. This study identified 10 genes of the I. indigotica RBOH gene family (IiRBOH) and divided them into five subfamilies (I-V). Genes within the same class show conserved structural features and similar amino acid sequences. Analysis of CRE suggested that IiRBOH genes might play roles in growth and development, metabolism, hormone regulation, and stress response. Two physiological indicators of I. indigotica treated with salt for different days were detected. It was found that the content of H₂O₂ in the I. indigotica tissue first increased, then decreased and increased again. The catalase activity also showed a trend of first increasing and then decreasing. The qRT-PCR results showed that these IiRBOH genes showed different expression patterns in response to salt stress, and some of these genes may be involved in the resistance of I. indigotica to salt stress. Through RT-PCR analysis and screening on the PlantCARE website, it was found that IiRBOHA and IiRBOHC not only possess W-box CRE but also exhibit high expression under salt stress. Y1H experiments were conducted with the WRKY genes predicted by phylogenetic analysis to regulate salt stress potentially, and it was discovered that IiWRKY6 and IiWRKY54 can directly activate the transcription of the IiRBOHA gene promoter. This study preliminarily explored the mechanism by which the RBOH gene in I. indigotica mediates H₂O₂ to resist salt stress, thus laying a foundation for further research on the biological functions of the RBOH gene in I. indigotica.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"52"},"PeriodicalIF":5.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the role of autophagy and antioxidants in anther and pistil responses to heat stress in rapeseed (Brassica napus L.).","authors":"Valiollah Mohammadi, Ahmad Rezaeizadeh, Behnam Mondak, Abdolrahman Rasoulnia, José Domínguez-Figueroa, Laura Carrillo, Gara Romero-Hernandez, Joaquin Medina","doi":"10.1007/s00299-025-03437-6","DOIUrl":"10.1007/s00299-025-03437-6","url":null,"abstract":"<p><strong>Key message: </strong>Enhanced antioxidant enzymes activity, particularly superoxide dismutase and catalase, along with autophagy process in reproductive organs, can improve the resilience of rapeseed to heat stress, thereby securing crop yield in the face of global warming. Climate change and global warming have increasingly influenced yield and quality of rapeseed (Brassica napus) almost all across the world. The response of reproductive organs to high-temperature stress was studied in two rapeseed varieties, SAFI5 and DH13 with contrasting levels of heat stress tolerance. Pollen germination, viability, and seed set showed a significant reduction in the heat-sensitive variety (DH13). Superoxide quantification revealed higher accumulation in heat-sensitive variety, leading to decreased seed formation and floret fertility most probably due to declined pollen viability and stigma receptivity. Further microscopic analysis of the anther and pistil demonstrated a significant overlay between the damaged areas and the location of O₂^- accumulation. The sensitive variety showed higher O₂^- accumulation and a wider damage area than the tolerant one, suggesting that superoxide could incapacitate anther and pistil due to structural injury. Moreover, the activity levels and expression of superoxide dismutase and catalase antioxidant enzymes were significantly higher in the anther and pistil of the tolerant variety. Histochemical analysis also indicated markedly higher autophagosome formation in tolerant variety's anther and pistil. Consistently, the expression levels of autophagy and ubiquitin-proteasome system (UPS)-related genes including BnATG8d, BnEXO70B, BnATl1 4A, and BnNBR1, as well as ubiquitin-activating enzyme E1, were higher in both reproductive organs of the tolerant variety. Interestingly, the areas of autophagosome formation overlapped with the areas in which higher superoxide accumulation and structural changes happened, suggesting a specific role of autophagy in oxidative stress response.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"51"},"PeriodicalIF":5.3,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11805782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel insight of the SVP gene involved in pedicel length based on genomics analysis in cherry.","authors":"Wei Tan, Pengyu Zhou, Xiao Huang, Zefu Wang, Ruyu Liao, Faisal Hayat, Xiaoan Wang, Zhaojun Ni, Ting Shi, Xiaqing Yu, Huiqin Zhang, Feng Gao, Yang Bai, Daouda Coulibaly, Ouma Kenneth Omondi, Zhihong Gao","doi":"10.1007/s00299-025-03439-4","DOIUrl":"10.1007/s00299-025-03439-4","url":null,"abstract":"<p><strong>Key message: </strong>PcSVP was identified based on Prunus conradinae genome and was further overexpressed in A. thaliana to comfirm it was a key factor in flower development, causing the pedicels elongation. Prunus conradinae is an endemic plant resource in China with high ornamental and economic values. To generate useful genomic resources for expanding insights into the evolutionary history of this important plant, the chromosome-level genome and organelle genomes of P. conradinae are de novo assembled and functionally annotated. The chromosome-level haploid genome of autotetraploid P. conradinae was assembled with 262.79 Mb with 27,802 protein-coding genes annotated. The complete chloroplast and mitochondrial genome of P. conradinae are found to be 157,715 bp and 434,334 bp, respectively. According to evolutionary analysis, P. conradinae was closely related to P. serrulata and P. yedoensis, and they diverged from their common ancestor approximately 6.0 million years ago. There were 108 gene families that significantly expanded during P. conradinae evolution and 56 shared positively selected genes. Selective sweep analysis based on the whole-genome resequencing of wild cherries from Fujian and Zhejiang indicated that genes involved in flower development and stress responses were potentially under selection. Pedicel length varied greatly among Prunus species and was a significant identifying characteristic. Ectopic overexpression of PcSVP in Arabidopsis thaliana suggested that it was a key factor in flower development, causing the sepals curling and pedicels elongation. These findings will contribute to the discovery of key functional genes involved in the agronomic or biological traits of P. conradinae, as well as the future development, utilisation and germplasm conservation of wild cherries.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"50"},"PeriodicalIF":5.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential role of rice ERF101 in the perception of TAL effectors and immune activation mediated by the CC-BED NLR Xa1.","authors":"Ayaka Yoshihisa, Satomi Yoshimura, Junwen Zhou, Kei Nishikawa, Koji Yamaguchi, Tsutomu Kawasaki","doi":"10.1007/s00299-025-03436-7","DOIUrl":"10.1007/s00299-025-03436-7","url":null,"abstract":"<p><strong>Key message: </strong>Rice CC-BED NLR Xa1 recognizes TAL effectors through the interaction between ERF101 and TAL effectors. The rice Xa1 gene encodes a nucleotide-binding leucine-rich repeat receptor with an N-terminal coiled coil-zinc finger BED (CC-BED) domain. Xa1 recognizes the transcription activator-like (TAL) effectors of Xanthomonas oryzae pv. oryzae (Xoo) in the nucleus, triggering a number of immune responses, including hypersensitive cell death. We previously discovered that the rice transcription factor ERF101 directly interacts with Xa1, and functions as a positive regulator of Xa1-dependent immunity. However, the involvement of ERF101 in Xa1-induced immunity remains unclear. We herein demonstrated that the expression of the CC-BED domain in rice protoplasts inhibited Xa1-induced cell death. However, the CC-BED^C165A,C168A domain which has mutations of cysteine residues conserved in the zinc-finger motifs of BED domains and is essential for forming tetrahedral coordination geometry, failed to inhibit cell death or interact with ERF101. Therefore, Xa1-induced cell death appears to depend on the interaction between the BED domain and ERF101. In addition, we generated transgenic plants overexpressing N-terminal or C-terminal FLAG-tagged ERF101. FLAG-ERF101 transgenic plants exhibited reduced levels of Xa1-mediated immunity against Xoo, even though the overexpression of ERF101-FLAG or non-tagged ERF101 enhanced immunity. This result was consistent with the CC-BED domain interacting with C-terminal tagged ERF101, but not N-terminal tagged ERF101, whereas N-terminal and C-terminal tagged ERF101 both interacted with TAL effectors. Therefore, the interaction between the BED domain and ERF101 appears to be essential for the recognition of TAL effectors by Xa1.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"49"},"PeriodicalIF":5.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high temperature responsive UDP-glucosyltransferase gene OsUGT72F1 enhances heat tolerance in rice and Arabidopsis.","authors":"Yuqing Ma, Shuman Zhao, Xinmei Ma, Guangrui Dong, Chonglin Liu, Yi Ding, Bingkai Hou","doi":"10.1007/s00299-025-03438-5","DOIUrl":"10.1007/s00299-025-03438-5","url":null,"abstract":"<p><strong>Key message: </strong>OsUGT72F1 enhances heat tolerance in plants by improving ROS scavenging and modifying multiple metabolic pathways, under the regulation of transcription factors OsHSFA3 and OsHSFA4a. High temperature is one of the most critical environmental constraints affecting plant growth and development, ultimately leading to yield losses in crops such as rice (Oryza sativa L.). UDP (uridine diphosphate)-dependent glycosyltransferases (UGTs) are believed to play crucial roles in coping with environmental stresses. However, the functions for the vast majority of UGTs under high temperature stress remain largely unknown. In this study, we isolated and characterized a high temperature responsive UDP-glycosyltransferase gene OsUGT72F1 in rice. Our findings demonstrated that overexpression of OsUGT72F1 enhanced heat-stress tolerance, while the mutant plants displayed a sensitive phenotype under the same stress conditions. Ectopic expression of OsUGT72F1 in Arabidopsis thaliana also conferred improved heat tolerance to the plants. Further investigation revealed that OsUGT72F1 reduced the generation of reactive oxygen species (ROS) and boosted the activity of antioxidant enzymes, thereby alleviating oxidative damage under heat-stress conditions. Moreover, transcriptomic analysis indicated that the action of OsUGT72F1 leads to the upregulation of multiple metabolic pathways including phenylpropanoid biosynthesis, zeatin biosynthesis, and flavonoid biosynthesis. In addition, the upstream regulatory mechanism of the OsUGT72F1 gene has been identified. We found that the transcription factors OsHSFA3 and OsHSFA4a can bind to the OsUGT72F1 promoter and enhance its transcription level. Together, this study revealed that the glycosyltransferase gene OsUGT72F1 plays a vital role in the adaptive adjustment of high temperature stress in plants, revealing a new heat tolerance pathway and providing a promising gene candidate for the breeding of heat-resistant crop varieties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"48"},"PeriodicalIF":5.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pectin methylesterase inhibitor 58 negatively regulates ray petal elongation by inhibiting cell expansion in Gerbera hybrida.","authors":"Najin Xiao, Xiaohui Lin, Shuyi Situ, Xiaojing Wang, Yaqin Wang","doi":"10.1007/s00299-025-03434-9","DOIUrl":"10.1007/s00299-025-03434-9","url":null,"abstract":"<p><strong>Key message: </strong>GhPMEI58 is a functional pectin methylesterase inhibitor, negatively regulates ray petal elongation by inhibiting cell expansion. Petal size plays a crucial role in the quality of ornamental flowers, which is largely determined by cell expansion. However, the molecular mechanisms controlling this trait are still unknown. In this study, we reported GhPMEI58 (pectin methylesterase inhibitor 58), a functional pectin methylesterase inhibitor, negatively regulates ray petal elongation by inhibiting cell expansion. The expression of GhPMEI58 was found to be highest in ray florets and increased with ray petal elongation. Subcellular localization analysis showed that the GhPMEI58 protein is localized on the cell membrane. By stably overexpressing GhPMEI58 in Arabidopsis, we detected a decrease in pectin methylesterase activity in roots and flowers, and an increase in the degree of pectin methylation, indicating that GhPMEI58 functions as a pectin methylesterase inhibitor. Further transient overexpression and virus-induced gene silencing experiments in gerbera ray florets confirmed that GhPMEI58 is a negative regulator of ray petal elongation. Additionally, the activity assay of pectin methylesterase showed it has a trend of first rising and then falling during ray floret development. This study explores the regulatory mechanism of ray petal elongation in gerbera from the perspective of cell wall methylesterification modification, providing valuable information for improving gerbera varieties and breeding superior cultivars.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"47"},"PeriodicalIF":5.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of GRAS genes in passion fruit (Passiflora edulis Sims) revealed their roles in development regulation and stress response.","authors":"Xinkai Cai, Denglin Li, Chaojia Liu, Jiayi Chen, Xiuqing Wei, Sitong Hu, Lin Lu, Shengzhen Chen, Qinglong Yao, Shiyu Xie, Xiaowen Xu, Ruoyu Liu, Yuan Qin, Ping Zheng","doi":"10.1007/s00299-025-03432-x","DOIUrl":"10.1007/s00299-025-03432-x","url":null,"abstract":"<p><strong>Key message: </strong>Twenty-nine GRAS genes were identified in passion fruit, the N-terminal regions and 3D (three-dimensional) structures were closely related with their tissue-specific expression patterns. Candidate PeGRASs for enhancing stress resistance were identified. Passion fruit (Passiflora edulis Sims) is a tropical fruit crop with significant edible and ornamental value, but its growth and development are highly sensitive to environmental conditions. The plant-specific GRAS gene family plays critical roles in regulating growth, development, and stress responses. Here, we performed the first comprehensive analysis of the GRAS gene family in passion fruit. A total of 29 GRAS genes were identified and named PeGRAS1 to PeGRAS29 based on their chromosomal locations. Phylogenetic analysis using GRAS proteins from passion fruit, Arabidopsis, and rice revealed that PeGRAS proteins could be classified into 10 subfamilies. Compared to Arabidopsis, passion fruit lacked members from the LAS subfamily but gained one GRAS member (PeGRAS9) clustered with the rice-specific Os4 subfamily. Structural analysis performed in silico revealed that most PeGRAS members were intron less and exhibited conserved motif patterns near the C-terminus, while the N-terminal regions varied in sequence length and composition. Members within certain subfamilies including DLT, PAT1, and LISCL with similar unstructured N-terminal regions and 3D structures, exhibited similar tissue-specific expression patterns. While PeGRAS members with difference in these structural features, even within the same subfamily (e.g., DELLA), displayed distinct expression patterns. These findings highlighted that the N-terminal regions of GRAS proteins may be critical for their specific functions. Moreover, many PeGRAS members, particularly those from the PAT1 subfamily, were widely involved in stress responses, with PeGRAS19 and PeGRAS26 likely playing roles in cold tolerance, and PeGRAS25 and PeGRAS28 in drought resistance. This study provides a foundation for further functional research on PeGRASs and offers potential candidates for molecular breeding aimed at enhancing stress tolerance in passion fruit.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"46"},"PeriodicalIF":5.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}