Plant Cell Reports最新文献

{"title":"Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.","authors":"Qingtong Yang, Gang Wang, Jing Ma, Heying Zhou, Lang He, Chunpu Qu","doi":"10.1007/s00299-025-03516-8","DOIUrl":"10.1007/s00299-025-03516-8","url":null,"abstract":"<p><strong>Key message: </strong>Overexpressed AlaAT3 in Populus enhances ammonium tolerance by modulating carbohydrate metabolism, nitrogen metabolism, and antioxidant system-related metabolic processes. Alanine aminotransferase (AlaAT) is a critical enzyme involved in the nitrogen assimilation process in plant cells, catalyzing the reversible transfer of an amino group from alanine to α-ketoglutarate. This reaction is essential for maintaining metabolic homeostasis. Previous studies have suggested that AlaAT plays a role in alleviating ammonium toxicity in plants. To investigate this hypothesis, transgenic Populus × xiaohei plants overexpressing AlaAT3 were generated, and their phenotypic, physiological, and transcriptional traits were compared with those of wild-type (WT) plants. Under treatment with 3 mM NH₄⁺ ammonium nitrogen, the transgenic plants exhibited significantly enhanced root biomass. Compared with WT plants, the transgenic lines demonstrated higher activities of GS, SOD, and CAT enzymes, while POD activity was notably reduced. Levels of soluble protein, free amino acids, sucrose, starch, soluble sugars, and proline were significantly elevated, whereas concentrations of O₂^-, and NH₄⁺ were markedly reduced. Transcriptomic analysis revealed significant enrichment in glutathione metabolism, peroxisome, nitrogen metabolism, and starch and sucrose metabolism pathway in the transgenic plants, with corresponding genes displaying notable transcriptional changes. Regulatory network analysis identified key transcription factors, including WRKY53, DOF3.4, and DOF1.5, as potential regulators of ammonium toxicity resistance in these transgenic lines. These findings demonstrate that AlaAT3 overexpression enhances Populus × xiaohei tolerance to NH₄⁺ by modulating glutathione metabolism, peroxisome, nitrogen metabolism, and starch and sucrose metabolism pathway. This study provides candidate genes and lays a strong foundation for future research into the mechanisms underlying NH₄⁺ tolerance in Populus plants overexpressing AlaAT3.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"126"},"PeriodicalIF":5.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RcPLATZ8 as a novel negative regulator of flowering in Rosa chinensis.","authors":"Yifang Peng, Qi Li, Yao Gong, Qian Yang, Qijing Dong, Yu Han","doi":"10.1007/s00299-025-03513-x","DOIUrl":"10.1007/s00299-025-03513-x","url":null,"abstract":"<p><strong>Key message: </strong>Comprehensive analysis of the RcPLATZ gene family in Rosa chinensis reveals RcPLATZ8 as a novel negative regulator of flowering, offering insights for targeted breeding to manipulate flowering traits. Flowering regulation in Rosa chinensis is essential for improving ornamental and commercial traits, but its molecular mechanisms remain poorly understood. In this study, we identified and characterized ten members of the PLANT AT-RICH SEQUENCE AND ZINC-BINDING (PLATZ) protein family in R. chinensis through genome-wide analysis and protein domain validation using the Pfam database. Among these, we focused on RcPLATZ8, a novel negative regulator of flowering. Expression analysis via RT-qPCR revealed that RcPLATZ8 is predominantly expressed in floral organs, including stamens, pistils, and petals, and exhibits significant responsiveness to key plant hormones, such as abscisic acid (ABA), gibberellins (GA), and jasmonic acid (JA). Functional assays showed that overexpression of RcPLATZ8 in Arabidopsis resulted in delayed flowering and increased leaf number, whereas silencing RcPLATZ8 in R. chinensis led to early flowering. Furthermore, Weighted Gene Co-expression Network Analysis (WGCNA) identified that RcPLATZ8 is part of the 'red module,' which is strongly associated with flowering-time regulatory genes, including SHORT VEGETATIVE PHASE (SVP). These findings provide new insights into the molecular regulation of flowering in roses, demonstrating that RcPLATZ8 may plays a key role in integrating hormonal signals and floral development. Our study not only expands the functional understanding of the PLATZ family but also offers potential strategies for molecular breeding aimed at improving flowering traits for horticultural applications.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"125"},"PeriodicalIF":5.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional response of cultivated peanut (Arachis hypogaea L.) roots to salt stress and the role of DNA methylation.","authors":"Shree P Pandey, Chen Chen, Shivam Singh, Jalak N Maniar, Avinash Mishra, Suman Bakshi, V K Mishra, Sandeep Sharma","doi":"10.1007/s00299-025-03515-9","DOIUrl":"10.1007/s00299-025-03515-9","url":null,"abstract":"<p><strong>Key message: </strong>Our study unravels a complex multi-layered molecular response of peanut roots to salinity, where reprograming of gene-expression is partly executed by changes in methylome via RdDM pathway and exerted through transcription factors. Peanut (Arachis hypogaea L.) is a major oilseed crop of global importance, whose production is severely impacted by salinity. Here, we have explored the transcriptional response of peanut roots to salinity stress using deep sequencing. Further, we have unravelled the salinity-induced changes in peanut root methylome. When peanut seedlings were grown under high-salt conditions for 7 days, their root and shoot growth was significantly impaired. A large-scale transcriptional reprogramming was recorded where 1926 genes were down- and 3260 genes were up-regulated due to salt stress in peanut roots. The molecular response of peanut root comprised several layers of regulators, which included the genes related to ion transport, osmolyte accumulation, signal transduction, and salt stress-responsive genes. Several negative regulators are also differentially expressed in peanut roots, which may contribute to its susceptibility. This response is regulated by a large number of transcription factors (TFs) and epigenetically by changes in DNA methylation. The DNA methylation changes in roots were highly complex and context dependent when exposed to salt stress. An inverse relationship between the changes in gene expression and methylation status was partially observed for several important gene sets and TFs. A treatment with 5'-azacytidine recovered the inhibitory impact of salt stress in peanut roots. Thus, a complex multilayered molecular response to salinity in peanut roots was observed. A part of this response may be modulated by the reprogramming of RNA-directed DNA methylation pathway. This investigation also serves as a resource for future gene-mining and methylation studies for improving peanut resistance to salt stress.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"124"},"PeriodicalIF":5.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing genetic transformation efficiency of melon (Cucumis melo L.) through an extended sucrose-removal co-culture.","authors":"Xiang Li, Chenchen Cao, Ying Liu, Pablo Bolaños-Villegas, Jiyu Wang, Ranran Zhou, Juan Hou, Qiong Li, Wenwen Mao, Panqiao Wang, Lili Li, Chen Luo, Junlong Fan, Yan Guo, Zhiqiang Cheng, Jianbin Hu","doi":"10.1007/s00299-025-03521-x","DOIUrl":"10.1007/s00299-025-03521-x","url":null,"abstract":"<p><strong>Key message: </strong>The genetic transformation efficiency of melon was elevated by extending co-culture duration and removing sucrose from the medium, and a gene editing tendril-less mutant was generated via this optimized transformation. In plants, Agrobacterium-mediated transformation (AMT) is a valuable technique for characterizing gene function and developing varieties with new traits. However, melon, as a cash crop, has proven to be recalcitrant to AMT. During AMT, the co-culture phase is crucial for the successful integration of T-DNA into the host genome by Agrobacterium tumefaciens (A. tumefaciens). To enhance the AMT efficiency in melon, we optimized the co-culture regime by extending the co-culture duration and removing sucrose from the medium. Extending the co-culture duration to 7 days, compared to the usual 2 to 4 days, allowed A. tumefaciens to infect melon explants at its optimal capacity. The removal of sucrose not only prevented excessive proliferation of A. tumefaciens during the extended culture but also reduced the triggering of a defense response in melon explants. Compared to the sucrose-addition co-culture for 4 days, sucrose-removal co-culture for 7 days increased the efficiency of melon transformation by 14 folds. In addition, this optimized co-culture has a synergistic effect with AtGRF5 overexpression on enhancing AMT in melon. Using this optimized transformation protocol, we successfully obtained tendril-less melon plants by knocking out CmTCP1 gene via gene editing, which holds significant breeding potential. The transformation method detailed in this study may serve as a robust tool for gene biology research and plant breeding in melons and may potentially lead to enhanced AMT in other plant species.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"123"},"PeriodicalIF":5.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the trinity: unraveling a fourth clade in the PEBP gene family in plants.","authors":"Miguel A Burton, Carlos E Rodríguez-López, José E Cetz-Chel, Rafael Urrea-López, Alejandro Pereira-Santana","doi":"10.1007/s00299-025-03505-x","DOIUrl":"10.1007/s00299-025-03505-x","url":null,"abstract":"<p><strong>Key message: </strong>Proposal for a new fourth PEBP gene group (SFT-like) in a genomic context different from 21 the other three. FT/TFL groups evolved from MFT, but then became sub-, neo-functionalized. The phosphatidylethanolamine-binding protein (PEBP) gene family plays crucial roles in plant development, principally involved in flowering time regulation and seed development. Traditionally, PEBP genes are classified into three clades: MOTHER OF FT AND TFL1 (MFT), FLOWERING LOCUS T (FT), and TERMINAL FLOWER 1 (TFL). We used phylogenomic and microsynteny network analyses to explore the PEBP family across 275 plant genomes from different lineages. The phylogenetic tree of the identified 3707 PEBP proteins allows us to visualize a fourth clade within the PEBP family. This new clade, named SFT (Sibling of FT/TFL), is closely related to the MFT clade but sister to the branch point of FT/TFL subfamilies, suggesting a long-standing evolutionary divergence. In addition, the SFT subfamily is in a different genomic context, whereas FT and TFL share a common origin with MFT. Motif analyzes show differences between this new clade and those already reported, suggesting functions other than flowering or seed development. The Ka/Ks analysis also suggests that SFT clade had fewer duplication events, so these genes could have an important function for the plant that had not yet been elucidated. These findings offer new insights into the evolutionary history and functional diversification of PEBP genes in plants. This study provides an update on the classification of the PEBP family. By understanding the syntenic relationships and evolutionary dynamics within the PEBP family, this research sets the stage for future functional studies on PEBP genes in plant biology, particularly the recently identified SFT clade.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"122"},"PeriodicalIF":5.3,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping stress memory: genetic and epigenetic insights into combined drought and heat tolerance in barley.","authors":"Amr Elkelish, Ahmad M Alqudah, Abdulrahman M Alhudhaibi, Hussain Alqahtani, Essa M Saied, Andreas Börner, Samar G Thabet","doi":"10.1007/s00299-025-03501-1","DOIUrl":"10.1007/s00299-025-03501-1","url":null,"abstract":"<p><strong>Key message: </strong>Unveiling genetic and epigenetic mechanisms in barley, this study maps stress memory under combined drought and heat, advancing resilience breeding for climate-adaptive crop improvement. Barley is one of the world's most important cereal crops and is increasingly threatened by concurrent drought and heat stress, two major environmental factors intensified by climate change. In our study, we employed a genome-wide association scan (GWAS) to investigate the concept of \"stress memory,\" wherein barley plants exposed to previous stress events exhibit enhanced responses to subsequent ones. We evaluated key agronomic traits, such as plant height, spike length, grain number, and thousand kernel weight along with biochemical markers such as chlorophyll content, proline, and soluble proteins across three generations under combined drought and heat stress. This approach encompassed transgenerational and intergenerational stress memory and a third generation that could reveal the potential cumulative effects of combined drought and heat stress. Our findings demonstrated a significant increase in metabolites specifically proline and soluble proteins in third-generation barley plants compared to those exposed to stress for only one or two generations. Through GWAS analysis, we identified 332 highly significant SNP markers clustered within 14 genomic regions on chromosomes 2H, 3H, 4H, 5H, and 7H. These regions are associated with all evaluated physiological and morphological traits under stress that harbor several potential candidate genes implicated in regulating complex signaling pathways, reactive oxygen species scavenging, and energy metabolism processes essential for mitigating the impacts of drought and heat. These results underscore the intricate nature of barley's stress tolerance mechanisms and highlight the potential for integrating genomics, epigenomics, and advanced phenotyping approaches into breeding programs.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"120"},"PeriodicalIF":5.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates plant stem cell maintenance under oxidative stress.","authors":"Jiaqi Qiu, Minghuang Chen, Zheqi Cai, Xiaofen Chen, Zelong Pang, Hao Chen, Tao Huang","doi":"10.1007/s00299-025-03507-9","DOIUrl":"10.1007/s00299-025-03507-9","url":null,"abstract":"<p><strong>Key message: </strong>GAPDH regulates plant stem cell maintenance. WUSCHEL (WUS) and WUSCHEL-RELATED HOMEOBOX (WOX) family proteins are vital for maintaining the homeostasis of stem cells, which is necessary for the continuous growth and the development of plants. Plants frequently encounter environmental stress that can lead to an increase in reactive oxygen species, such as hydrogen peroxide (H₂O₂). However, the exact ways in which plant stem cells sense and respond to H₂O₂ signals remain unclear. This research indicates that cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) helps regulate stem cell maintenance in Arabidopsis in response to H₂O₂. Hydrogen peroxide causes the relocation of two cytosolic GAPDH proteins, GAPC1 and GAPC2, from the cytoplasm to the nucleus. These isoforms interact with WUS/WOX proteins and modulate the expression of the WUS/WOX gene by binding to its promoter. When the expression of GAPC1 and GAPC2 is decreased, stem cell homeostasis and overall plant growth become more sensitive to H₂O₂. Thus, cytosolic GAPDH may serve as a sensor for H₂O₂, influencing the maintenance of plant stem cells under oxidative stress.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"121"},"PeriodicalIF":5.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-based modulation of V-PPase expression in rice improves grain quality and yield under high nighttime temperature.","authors":"Flávia Barbosa Silva Botelho, Soumen Nandy, Vibha Srivastava","doi":"10.1007/s00299-025-03504-y","DOIUrl":"10.1007/s00299-025-03504-y","url":null,"abstract":"<p><strong>Key message: </strong>Transcriptional modulation of the vacuolar H⁺ translocating pyrophosphatase expressed specifically in the endosperm and reproductive tissue of rice improves its spikelet fertility and reduces grain chalkiness under high nighttime temperature.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"119"},"PeriodicalIF":5.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlDCD and SlLCD increased the salt tolerance in tomato seedlings by enhancing antioxidant and photosynthesis capacity.","authors":"Xinfang Chen, Dengjing Huang, Xiaoling Man, Ailing Li, Hua Fang, Siting Lu, Di Yang, Weibiao Liao","doi":"10.1007/s00299-025-03509-7","DOIUrl":"10.1007/s00299-025-03509-7","url":null,"abstract":"<p><strong>Key message: </strong>Using gene silence and heterologously overexpression, hydrogen sulfide synthesis-related genes l-cysteine desulfhydrase and d-cysteine desulfhydrase have been shown to enhance salt tolerance in tomato seedlings. Hydrogen sulfide (H₂S) plays an important role in alleviating abiotic stress. L-Cysteine desulfhydrase (LCD) and D-cysteine desulfhydrase (DCD) are two important H₂S synthesis enzymes. Until now, whether and how SlDCD and SlLCD increase salt tolerance in plant are still unknown. Here, we explored the effects of SlDCD and SlLCD on salt tolerance in tomato seedlings by silencing SlDCD and SlLCD and heterologously overexpressing SlDCD and SlLCD. In tomato seedlings, exogenous sodium hydrosulfide (NaHS, a H₂S donor) increased salt tolerance while decreasing H₂S synthesis-related enzyme activity, endogenous H₂S levels, and H₂S synthesis-related gene expression. Silencing SlDCD and SlLCD inhibited tomato seedling growth under salt stress, increased relative conductivity, MDA, H₂O₂, O₂^-, Pro, and carotenoid content, Ci and NPQ. In contrast, it decreased the activity of antioxidant enzymes (POD, SOD, CAT and APX) and the expression of related genes (POD, SOD, CAT and APX), chlorophyll content, photosynthetic parameters (Pn, Gs and Tr) and fluorescence parameters (Fv/Fm, φPSII and qP), while exogenous NaHS considerably mitigated the adverse impacts of salt stress in SlDCD and SlLCD silenced-tomato seedlings. Overexpression of SlDCD and SlLCD in Arabidopsis significantly enhanced plant salt tolerance. Taken together, our results indicate that SlDCD and SlLCD could enhance the antioxidant activity and photosynthesis capacity under salt stress, which results improving salt tolerance in tomato seedlings.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"117"},"PeriodicalIF":5.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for maintaining the release of co-suppression and maximally restoring the RDR6 expression.","authors":"Na Li, Hailan Wang, Jiayin Wen, Xiangling Liu, Meng Zhang","doi":"10.1007/s00299-025-03508-8","DOIUrl":"10.1007/s00299-025-03508-8","url":null,"abstract":"<p><strong>Key message: </strong>Tissue-specific RDR6 compensation rescues Arabidopsis defects while maintaining seed polyunsaturated fatty acid accumulation, balancing co-suppression relief and key trait retention for modular engineering. The transgene-induced co-suppression of fatty acid desaturase 2 (FAD2) can be effectively released in rdr6 mutant, enabling a significant increase in polyunsaturated fatty acid (PUFA) content in seeds. However, the global suppression of RNA-dependent RNA polymerase 6 (RDR6) compromises plant growth and disease resistance. To address this limitation, we developed a spatiotemporal compensation strategy by restoring RDR6 expression in non-seed tissues using tissue-specific promoters while maintaining its low expression during seed maturation. To implement this goal, we identified P_BnTC06, a Brassica napus promoter, through transcriptomic data mining and functional characterization. GUS staining revealed that the P_BnTC06 promoter drives strong gene expression in vegetative tissues (e.g., leaves, stems, and flowers) but exhibits negligible activity in mid- to late-stage-developing seeds. We introduced P_BnTC06::RDR6 into Pha::AtFAD2/rdr6-11, the previously established high- PUFA Arabidopsis line. This intervention rescued the rdr6 mutant phenotype (characterized by gracile, downward-curling leaves) to wild-type morphology and restored RDR6 expression across non-seed tissues, while maintaining minimal expression in middle and late developing seeds. Crucially, FAD2 transcript levels remained at a high level during late seed development, resulting in sustained high PUFA accumulation in mature seeds. This strategy establishes a practical strategy to circumvent transgene co-suppression and proposes a modular framework for precision breeding of complex traits in crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"118"},"PeriodicalIF":5.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}