Plant Cell Reports最新文献

{"title":"Recombinant production of spider silk protein in Physcomitrella photobioreactors.","authors":"Maryam Ramezaniaghdam, Lennard L Bohlender, Juliana Parsons, Sebastian N W Hoernstein, Eva L Decker, Ralf Reski","doi":"10.1007/s00299-025-03485-y","DOIUrl":"https://doi.org/10.1007/s00299-025-03485-y","url":null,"abstract":"<p><strong>Key message: </strong>We report the successful moss-produced recombinant spider silk key protein component containing both the N- and the C-terminal domain. Spider dragline silk stands out as a remarkable biomaterial, representing one of nature's toughest fibres. Its strength rivals that of many synthetic fibres used commercially, rendering it applicable across various industrial and medical domains. However, its widespread utilisation requires cost-effective mass production. Biotechnology presents a promising avenue for achieving this goal, particularly through the production of recombinant dragline silk proteins in transgenic plant systems. This study aimed to assess the feasibility of producing one key protein component of dragline silk, MaSp1, from the western black widow spider, Latrodectus hesperus, the protein LhMaSp1, in the moss Physcomitrella (Physcomitrium patens). Here, we present the successful recombinant production of spider silk protein containing both the N- and C-terminal domains of LhMaSp1 in moss cells. The production of recombinant LhMaSp1 protein in Physcomitrella was performed in shake flasks and in five-litre photobioreactors and the correct synthesis of LhMaSp1 was proven via mass spectrometry. We estimate that the yield of recombinant spider silk protein in Physcomitrella bioreactors is above 0.82 mg/g fresh weight.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"103"},"PeriodicalIF":5.3,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem trichome polarity development in Gossypium hirsutum: insights into GhPRP gene regulation.","authors":"Huanying Deng, Longen Ma, Li Yu, Yanhao Zhao, Yurong Jiang, Junkang Rong","doi":"10.1007/s00299-025-03491-0","DOIUrl":"https://doi.org/10.1007/s00299-025-03491-0","url":null,"abstract":"<p><strong>Key message: </strong>Cotton stem trichomes exhibit a distinct polarity distribution, which may be regulated by GhPRP genes and temperature. Stem trichomes in cotton are essential for pest resistance and stress tolerance, yet their molecular regulation remains poorly understood. Significant differences in trichome number and length were observed under 25 °C and 30 °C, with more and longer trichomes at the first stem node under 25 °C. The side above the first true leaf (M side) showed more number of trichomes than the opposite side (L side), indicating polarity distribution. Transcriptome sequencing (RNA-seq) identified differentially expressed genes (DEGs), and 17 key DEGs were selected for further analysis, including 9 upregulated genes encoding proline-rich cell wall proteins (PRPs), flavonol synthase (FLS), prolyl endopeptidase (PREP), and diacylglycerol O-acyltransferase 3 (DGAT3). Quantitative real-time PCR (qRT-PCR) confirmed higher GhPRP expression on the M side. When GhPRP1, GhPRP2, or GhPRP10 was silenced using virus-induced gene silencing (VIGS) technique, trichome density decreased, and polarity was disrupted, highlighting their regulatory roles. Bioinformatics analysis revealed hormone signal transduction-related domains in PRP gene promoters, potentially linking them to trichome polarity regulation. This study advances understanding the mechanisms of trichome polarity distribution and offers insights for improving pest resistance and stress adaptation in cotton.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"102"},"PeriodicalIF":5.3,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought stress memory in maize: understanding and harnessing the past for future resilience.","authors":"Latif A Peer, Aijaz A Wani, Ajaz A Lone, Zahoor A Dar, Bilal A Mir","doi":"10.1007/s00299-025-03494-x","DOIUrl":"https://doi.org/10.1007/s00299-025-03494-x","url":null,"abstract":"<p><p>Maize (Zea mays L.), a cornerstone of global food security, faces significant challenges due to drought stress, which disrupts its growth, development, and productivity. This review synthesizes advances in our understanding of drought stress memory, a mechanism that enables maize to \"remember\" prior drought exposure through transcriptional, epigenetic, and physiological pathways. Key regulators, including transcription factors (ZmEREB24 and ZmNF-YC12) and epigenetic modifications (DNA methylation and histone acetylation), orchestrate stress-responsive pathways that ensure rapid adaptation to recurrent drought events. Complementing these molecular mechanisms, physiological adaptations, such as optimized root and leaf architecture, enhanced water-use efficiency, and antioxidant defenses, further strengthen drought tolerance. Practical applications, including molecular priming techniques (e.g., osmopriming, hydropriming, nanoparticles) and advanced genetic tools (CRISPR/Cas9, GWAS), promise scalable solutions for breeding drought-resilient maize varieties. Despite this progress, challenges remain, including genotype-specific variability, scalability, and trade-offs between resilience and yield. This review provides a roadmap for integrating laboratory discoveries with field-level practices, bridging molecular and agronomic innovations to address climate variability and ensure sustainable maize production and global food security.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"101"},"PeriodicalIF":5.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging chlorophyll fluorescence uncovers potato virus Y resistance in potato and its validation through viral quantification and yield loss studies.","authors":"Rakesh Belludi, Abhishek Sharma, Sat Pal Sharma, Gutha Venkata Ramesh, Santosh Gudi","doi":"10.1007/s00299-025-03489-8","DOIUrl":"https://doi.org/10.1007/s00299-025-03489-8","url":null,"abstract":"<p><strong>Key message: </strong>Chlorophyll fluorescence (CF) measurements have been demonstrated to be an efficient and non-invasive tool for identifying and developing PVY-resistant potato cultivars. The validity of CF measurements was confirmed through viral titer and yield-loss assays. In the quest to identify resistant sources for potato virus Y (PVY) within Indian potato germplasm, we developed a phenotyping approach leveraging plant physiological responses against PVY infection. The study evaluated 71 potato genotypes including cultivated and experimental clones, during the year 2021-2022 and 2022-23 through mechanical inoculation in experimental fields at the Punjab Agricultural University, Ludhiana. We employed a combination of serological and molecular screening, complemented with chlorophyll fluorescence (CF) measurements to classify resistant and susceptible genotypes. Out of 71 genotypes, 34 exhibited PVY resistance, with KP-16-19-14 being the highly resistant line with minimal yield loss (i.e., only 1.64% reduction) and undetectable viral titer. This genotype holds promise as a valuable resistance source for future breeding programmes. Our findings revealed that resistant genotypes maintained stable CF metrics and experienced minimal yield reductions (up to 5.15% only), with very low viral titer. In contrast, the photosynthetic efficiency was significantly declined in susceptible genotypes, which also experienced yield losses up to 58.84% with very high viral titer. Correlation coefficient and principal component analysis (PCA) revealed a strong association among the CF parameters, disease severity, viral titer, and yield losses. This emphasizes the utility of CF as a valuable tool for assessing resistance through physiological responses to PVY. Study demonstrates that photochemistry, heat dissipation, and fluorescence emission patterns of PS-II effectively differentiate resistant and susceptible genotypes. Moreover, this study highlights the potential of integrating physiological assessments with molecular diagnostics in large-scale preliminary screening to identify and develop PVY-resistant potato genotypes.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"100"},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RsWRKY44 participated in anthocyanin biosynthesis regulation in radish through interaction with RsMYB1a.","authors":"Biao Lai, Chenxi Gao, Li Jiang, Li Wen, Xushuo Zhang, Wei Shen, Yanling Yu, Hanbing Yang, Fabo Chen, Ping Fang, Lina Du","doi":"10.1007/s00299-025-03487-w","DOIUrl":"https://doi.org/10.1007/s00299-025-03487-w","url":null,"abstract":"<p><strong>Key message: </strong>RsWRKY44 transcription factor, associated with anthocyanin biosynthesis in different radish cultivars, highly facilitates the activation of RsCHI and RsUFGT genes through its interaction with RsMYB1a, thereby promoting anthocyanin production. The regulation of anthocyanin biosynthesis in radish is primarily controlled by RsMYB1a and RsbHLH4, while the involvement of other factors in this process is not well understood. This study identified a WRKY transcription factor, RsWRKY44, as a key player in anthocyanin biosynthesis regulation. The expression of RsWRKY44 showed a strong correlation with anthocyanin content across different radish cultivars. RsWRKY44 was found to be expressed in the nuclei and exhibit transactivation activity. It was observed that only when RsWRKY44 was co-expressed with RsMYB1a, anthocyanin accumulation was induced in tobacco leaves, while RsWRKY44 alone did not. Additionally, RsWRKY44, along with RsMYB1a, activated the expression of tobacco endogenous anthocyanin biosynthesis regulatory genes NtAN1a and NtAN1b, as well as the structural genes NtCHS, NtCHI, NtDFR, NtF3H, NtANS, NtUFGT in transgenic tobacco. BiFC, FLC, and DLA assays confirmed the interaction between RsWRKY44 and RsMYB1a leading to the activation of radish genes RsCHI and RsUFGT, promoting anthocyanin biosynthesis. This study sheds light on a new molecular mechanism of RsWRKY44 involved in anthocyanin biosynthesis regulation in radish.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"99"},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The light-responsive transcription factor SlBBX20 improves low-temperature resistance of Solanum lycopersicum by affecting photosynthetic capacity, antioxidant capacity, and osmotic adjustment.","authors":"Yongbo Ma, Manling Chen, Yingjin Xing, Zhenglun Li, Ting Zhou, Haijing Yan, Jiahui Yan, Songshen Hu, Guobin Li, Xiaohui Hu, Xiaojing Li","doi":"10.1007/s00299-025-03480-3","DOIUrl":"https://doi.org/10.1007/s00299-025-03480-3","url":null,"abstract":"<p><strong>Key message: </strong>Expression of SlBBX20 in tomato was significantly induced by low-temperature stress. SlBBX20 plays a positive role for tomato to resistance low-temperature stress. SIBBX20 gene plays an important role in enhancing low-temperature resistance in tomato seedlings. Under normal conditions, overexpression of SIBBX20 increased the net photosynthetic rate and leaf pigment content (chlorophyll a, chlorophyll b, and carotenoids), the expression of the anthocyanin synthase gene (SIDFR), and the content of anthocyanins in tomato, whereas the photosynthetic capacity, the expression of SIDFR, and the content of anthocyanins in VIGS-mediated SlBBX20-silenced materials did not differ significantly from those in the control. Under low-temperature stress, overexpression of SlBBX20 significantly decreased REC, MDA content, ROS level, and starch accumulation, and increased RWC, photosynthetic capacity, antioxidant enzyme activity, anthocyanin content, and soluble sugar content in tomato leaves. In addition, overexpression of SlBBX20 increased the expression of SlDFR, antioxidant enzymes (SlSOD, SlPOD, and SICAT), low-temperature resistance-related genes (SICBF2, SICBF3, and SlWHYI), starch degradation genes (SIAMY), and decrease the expression of starch synthesis genes (SISA2, SIUGD), which improved the antioxidant capacity, reduced the accumulation of starch, and improved the low-temperature tolerance of tomato seedlings. In conclusion, SlBBX20 enhanced tomato low-temperature tolerance by maintaining the photosynthetic capacity of leaves, reducing ROS and starch accumulation, and increasing antioxidant enzymes and soluble sugar content.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"97"},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A 294 kb deletion causes reduced leaflet size and biomass in pigeonpea.","authors":"Xipeng Ding, Shangzhi Wang, Jiajia Luo, Pandao Liu, Yongwei He, Xinyong Li, Xiaoyan Luo, Wei Hu","doi":"10.1007/s00299-025-03488-9","DOIUrl":"https://doi.org/10.1007/s00299-025-03488-9","url":null,"abstract":"<p><strong>Key message: </strong>BSA-seq and fine mapping revealed a 294 kb deletion on chromosome 9 regulating leaflet size and biomass in pigeonpea. Leaf size critically influences photosynthetic capacity, impacting organic matter production and biomass yield. This study reports the identification and characterization of a small leaflet mutant (sl1) in pigeonpea (Cajanus cajan) generated via aerial mutagenesis. Compared to the wild-type Qiongzhong, sl1 displayed significantly reduced leaf area, plant height, stem diameter, and biomass, characteristic of a dwarf phenotype. Genetic analysis confirmed a single recessive locus controlling the sl1 phenotype. Bulked segregant analysis sequencing (BSA-seq) and fine mapping identified the causal mutation as a 294 kb deletion encompassing 21 genes on chromosome 9. Transcriptomic analysis identified 1,039 differentially expressed genes (DEGs), indicating disruptions in, among others, plant hormone signaling pathways. Analysis of 28 target plant hormone metabolites revealed significant shifts in sl1 mutant compared to wild-type, including increased levels of strigolactone, methyl indole-3-acetate, and trans-zeatin-riboside, and decreases in gibberellin A3, N6-isopentenyladenine, and methyl jasmonate. Cytological analysis revealed a decreased cell number in sl1 leaves, contributing to the reduced leaflet size. Three candidate genes, CC09g01700, CC09g01705, and CC09g01707, within the deleted region were prioritized based on their altered expression patterns and their putative roles in leaf development. These findings elucidate the genetic regulation of leaf morphology and biomass in pigeonpea, offering potential targets for marker-assisted selection to enhance pigeonpea yield.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"98"},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mi- 1 gene is a key regulator of defence mechanisms and cellular gene dynamics in response to root-knot nematodes.","authors":"Treesa Thomas, Amar A Sakure, Sushil Kumar, Ankita Mishra, Suhail Ahmad, Yogesh M Rojasara, Mahesh B Vaja, Dipak A Patel","doi":"10.1007/s00299-025-03484-z","DOIUrl":"https://doi.org/10.1007/s00299-025-03484-z","url":null,"abstract":"<p><strong>Key message: </strong>Root-knot nematodes threaten tomato cultivation worldwide. This study confirms Mi- 1 gene's role in defence by inducing programmed cell death, lignin accumulation, and cellular remodelling in resistant plants. Root-knot nematodes, particularly Meloidogyne species, pose a significant economic threat to tomato cultivation globally. Despite the identification of various Mi genes, their mechanisms of action are not fully understood. This study aimed to elucidate the role of the Mi- 1 gene by analysing transcriptomic data from both resistant and susceptible tomato genotypes and by constitutively expressing Mi- 1 in a susceptible cultivar. Transcriptome analysis of leaf and root samples upon RKN infection showed that the Mi- 1 gene was exclusively present in the roots of resistant plants. Further to investigate its function Mi- 1 was constitutively expressed under the CaMV 35S promoter in a susceptible tomato cultivar. Structural analysis confirmed that the Mi- 1 protein primarily localized in the cytoplasm and lacked a transmembrane motif. Following transformation, a comparative gene expression of wild-type and transformed tomato plants with genes obtained from transcriptome, revealing significant up-regulation of cellular, plant defence, and programmed cell death (PCD) related genes in the transgenic lines. Notably, the roots of the transformed plants exhibited thickened root morphology and high lignin accumulation, correlating with the expression of lignin biosynthesis genes. These findings suggested that the Mi- 1 gene is not only involved in PCD but also activates various defence-related and cellular remodelling genes by depositing the lignin in the root cell to combat against nematode attack.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"96"},"PeriodicalIF":5.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-truncated version of OsGADs leads to higher GABA accumulation and stronger stress tolerance in Oryza sativa L. var. japonica.","authors":"Ummey Kulsum, Nadia Akter, Kazuhito Akama","doi":"10.1007/s00299-025-03477-y","DOIUrl":"10.1007/s00299-025-03477-y","url":null,"abstract":"<p><strong>Key message: </strong>Calmodulin binding domain truncation from OsGAD1 and OsGAD3 resulted in enhanced GABA accumulation, upregulated stress related genes, and improved tolerance to multiple abiotic stresses. Rice (Oryza sativa L.), a critical crop for global food security, faces significant challenges from abiotic stresses. Gamma-aminobutyric acid (GABA), synthesized by glutamate decarboxylase (GAD), plays a vital role in stress tolerance. Truncating the calmodulin-binding domain (CaMBD) in GAD enzymes enhances GAD activity and GABA production. In this study, we developed a hybrid line, Hybrid #78, by crossing two genome-edited lines, OsGAD1ΔC #5 and OsGAD3ΔC #8, with truncated CaMBD in OsGAD1 and OsGAD3, respectively. Hybrid #78 demonstrated significantly improved survival rates in cold (25%), salinity (33%), flooding (83%), and drought (83%) stress conditions, compared with wild-type Nipponbare (0-33%), OsGAD1∆C #5 (0-66%), and OsGAD3∆C #8 (0-50%). Hybrid #78 showed the highest GABA levels during stress, with increases of 3.5-fold (cold), 3.9-fold (salinity), 5-fold (flooding), and 5-fold (drought) relative to wild-type Nipponbare and up to 2-fold higher than that of the parent lines. RNA-seq analysis from shoot tissues in control conditions identified 975 differentially expressed genes between Hybrid #78 and wild-type Nipponbare, with 450 genes uniquely expressed in the hybrid. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that upregulation in nitrogen metabolism pathways likely contributes to enhanced GABA synthesis via increased glutamate production. Hybrid #78 also showed broader gene expression variability, suggesting enhanced adaptability to stress, especially upregulation of stress-related genes, such as OsDREB, OsHSP70, and OsNAC3. These findings highlight the potential of CaMBD truncation in OsGAD1 and OsGAD3 to develop rice lines with increased GABA accumulation and resilience to multiple abiotic stresses.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"95"},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought-induced 19 gene FvDi19-3 from woodland strawberry enhances drought and salt tolerance in transgenic Arabidopsis.","authors":"Jingjing Kong, Keli Qiu, Junyong Zhou, Debao Li, Lijuan Lu, Mao Liu, Shufang Zhu, Zhiyuan Ning, Qibao Sun","doi":"10.1007/s00299-025-03481-2","DOIUrl":"10.1007/s00299-025-03481-2","url":null,"abstract":"<p><strong>Key message: </strong>FvDi19-3 enhances drought and salt tolerance in Arabidopsis by promoting stomatal closure, improving the ability to scavenge reactive oxygen species, and increasing the expression of drought- or salt-responsive genes. Di19 (drought-induced 19) proteins play a crucial role in regulating plant development and various stress responses. However, a systematic identification and functional analysis of the Di19 gene family members in woodland strawberry has not yet been conducted. In this study, we identified four Di19 genes in woodland strawberry, and analyzed the phylogenetic tree, conserved protein domains, and gene structure. Cis-elements suggested that FvDi19 genes may be involved in plant development and stress responses. Gene expression analysis revealed diverse expression patterns of FvDi19 genes under different stress conditions, and overexpression of FvDi19 genes enhanced drought and salt tolerance in yeast. Transgenic and stress tolerance assays indicated that FvDi19-3 overexpression in Arabidopsis enhanced plant drought and salt tolerance by promoting stomatal closure, improving the plant's ability to scavenge reactive oxygen species and the expression of drought or salt-responsive genes. Further experiments indicated that FvWRKY42 and FvMYB114 can activate the expression of FvDi19-3, and expression of these three genes is dependent on the ABA signaling pathway. In conclusion, our study characterized the Di19 gene family in woodland strawberry and investigated the biological functions of FvDi19-3 in drought and salt tolerance, providing a basis for further functional studies of FvDi19 genes in responses to abiotic stress.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"94"},"PeriodicalIF":5.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}