Plant Cell Reports最新文献

{"title":"Harnessing the MYB51/SWEET20 module to increase soybean yield by facilitating sugar supply to sink organs.","authors":"Jiafang Shen, Dong Cao, Songli Yuan, Qingnan Hao, Hongli Yang, Yi Huang, Lihong He, Jialing Yuan, Zhonglu Yang, Shuilian Chen, Zhihui Shan, Wei Guo, Limiao Chen, Haifeng Chen, Xia Li, Chanjuan Zhang, Xinan Zhou","doi":"10.1007/s00299-025-03535-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03535-5","url":null,"abstract":"<p><strong>Key message: </strong>GmMYB51-GmSWEET20 plays an important role in soybean yield formation by promoting carbohydrates distribution and reducing flower and pod abscission. These insights provide a new molecular framework for soybean yield improvement. Enhancing the supply of carbohydrates into sink tissues is a promising strategy for improving soybean (Glycine max) yield. However, the underlying molecular mechanisms remain poorly understood in soybean. In this study, transcriptome comparison analysis of Zhongdou 29 (ZD29, a low-yielding variety) and Zhongdou 32 (ZD32, a high-yielding variety) identified a SWEET (Sugars Will Eventually be Exported Transporter) gene, GmSWEET20, which exhibited higher expression level in the petioles and stems of ZD32 and functioned as a sucrose and glucose transporter. Overexpression of GmSWEET20 resulted in increased pod number and higher yield by facilitating carbohydrate accumulation in sink tissues and reducing flower and pod abscission. While knock-down of GmSWEET20 and its three homologous genes with RNAi technology decreased pod number and yield. GmMYB51, which also exhibited higher expression level in ZD32 than in ZD29, could bind to the promoter of GmSWEET20 and activate its expression. We further confirmed overexpression of GmMYB51 also enhance efficient supply of carbohydrates to sink organs and increase pod number and yield through upregulation of GmSWEET20 expression. Taken together, our findings identified a novel regulatory module composed of GmMYB51 and GmSWEET20, which enhances carbohydrate supply in sink tissues, thereby leading to improved pod number and yield. These insights provide a molecular framework for yield improvement strategies of soybean.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"151"},"PeriodicalIF":5.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of PAMP-induced peptides and mechanistic insights into SlPIP2-mediated defense in tomato.","authors":"Ruirui Yang, Hongbo Wei, Jiaxuan Zhu, Zhiyuan Xue, Siya Zeng, Jun Meng, Yushi Luan","doi":"10.1007/s00299-025-03540-8","DOIUrl":"https://doi.org/10.1007/s00299-025-03540-8","url":null,"abstract":"<p><strong>Key message: </strong>SlPIP2 modulates the expression of PR genes, the activity of antioxidant enzymes, and the accumulation of defense-related metabolites in tomato, and concurrently contributes to enhanced resistance against Phytophthora infestans and Botrytis cinerea. Tomato (Solanum lycopersicum), as one of the most popular horticultural crops, is widely cultivated worldwide, however, its yield and quality is continually threatened by P. infestans. Plant peptides are engaged in the regulation of plant growth and immunity. PAMP-induced Peptides (PIPs) are new class of signaling peptides with diverse biologic roles in the regulation of plant defense responses. In this study, a total of seven SlPIP genes were identified in the tomato genome, and their expression profiles were analyzed under P. infestans infection. Among the SlPIP family members, SlPIP2 exhibited a significant response to pathogen infection. Through a combination of virus-induced gene silencing (VIGS) and gene overexpression, we demonstrated that SlPIP2 precursor (SlprePIP2) positively regulates tomato resistance. Notably, exogenous application of SlPIP2 enhanced plant defense responses, increasing resistance not only to P. infestans but also to B. cinerea, thereby highlighting its potential role in conferring broad-spectrum disease defense. To elucidate how SlPIP2 affected to tomato resistance, we performed transcriptomic analysis on tomato seedlings sprayed with H₂O and SlPIP2. GO and KEGG enrichment analyses revealed that SlPIP2 affects several key pathways including camalexin biosynthesis, plant-pathogen interactions, and MAPK signaling. Transcriptomic analysis further revealed that SlPIP2 regulates the expression of various transcription factors and hormone-related genes. In addition, SlPIP2 modulates the activity of antioxidant enzymes and accumulation of key defense-related metabolites. Collectively, our findings underscore the potential of SlPIP2 to enhance disease resistance in tomato, providing valuable insights and promising strategies for crop improvement and sustainable disease management.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"149"},"PeriodicalIF":5.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PgF3H gene enhances drought tolerance in transgenic Arabidopsis by regulating flavonoid biosynthesis and stress response.","authors":"Radha Shivhare, Priyamvada Mishra, Poorwa Kamal Badola, Puneet Singh Chauhan, Charu Lata","doi":"10.1007/s00299-025-03524-8","DOIUrl":"https://doi.org/10.1007/s00299-025-03524-8","url":null,"abstract":"<p><strong>Key message: </strong>Water stress stimulates plants to regulate flavonoid biosynthesis. Overexpression of the PgF3H gene increases flavonoid levels and drought tolerance in Arabidopsis, with stress-responsive elements in the PgF3H promoter indicating its role in drought response. Water stress significantly impairs plant growth and yield, but plants combat this through various strategies, including flavonoid biosynthesis regulation. Flavonoids, crucial secondary metabolites, aid in plant development and stress responses. Pearl millet, a drought-tolerant crop, produces high levels of secondary metabolites like flavonoids and anthocyanins via the phenylpropanoid pathway. Research indicates that flavonoid-encoding genes are prevalent in drought-tolerant pearl millet variants, hinting at their role in drought response, though their exact functions are not fully understood. This study highlights the essential role of pearl millet flavanone 3-hydroxylase (PgF3H) in flavonoid biosynthesis. To validate this function, PgF3H was expressed in flavonoid-deficient Arabidopsis mutant backgrounds: Atf3h (defective in flavanone 3-hydroxylase activity), Atans (mutated in anthocyanidin synthase, leading to impaired anthocyanin production), and Atanr (a regulatory mutant with altered anthocyanin accumulation). The PgF3H overexpression led to partial or complete restoration of flavonoid production in these mutants, reinforcing the gene's role in biosynthesis and drought resilience. In silico analysis of the PgF3H promoter revealed stress-responsive elements, and ProPgF3H::GUS expressing lines showed increased GUS expression with higher PEG concentrations. The in silico structure of PgF3H revealed a 2OG-Fe(II) oxygenase domain, crucial in the flavonoid biosynthetic pathway. In conclusion, PgF3H overexpression enhances drought tolerance in Arabidopsis, suggesting a potential strategy for improving crop drought resistance by manipulating flavonoid biosynthesis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"150"},"PeriodicalIF":5.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myosin XI coordinates ABA-induced stomatal closure via microtubule stability and ROS synthesis in drought-stressed Arabidopsis.","authors":"Haiyang Liu, Motoki Tominaga","doi":"10.1007/s00299-025-03538-2","DOIUrl":"10.1007/s00299-025-03538-2","url":null,"abstract":"<p><strong>Key message: </strong>Myosin XI contributes to ABA-triggered stomatal closure via reactive oxygen species signaling and microtubule remodeling, boosting drought tolerance in Arabidopsis.. ABA is a key hormone induced by drought stress, and it can regulate stomatal closure through the homeostasis of reactive oxygen species (ROS) and microtubule depolymerization in guard cells, which ultimately enhances plant drought resistance. In this study, we found that myosin XI double (2ko) and triple (3ko) mutants not only exhibited reduced drought resistance but also showed a weakened ABA response compared to the wild-type (WT). Through comprehensive phenotypic analysis and cellular observations, our experiments demonstrated that myosin XI plays a role in regulating ABA-induced ROS synthesis and microtubule depolymerization in guard cells, thereby facilitating stomatal closure, which minimizes leaf water loss while enhancing drought tolerance in Arabidopsis. This study provides novel insights into the role of myosin XI in abiotic stress responses by showing connections with fundamental ABA signaling pathways and broadens our understanding of myosin XI function in plants beyond its established roles in cytoplasmic streaming.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"147"},"PeriodicalIF":5.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12179000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional and phytohormonal responses to simulated animal grazing in Leymus chinensis.","authors":"Haiyan Li, Chunxu Zhou, Jiayuan Cheng, Han Wang, Xidan Zhang, Yingjie Yu, Lili Jiang","doi":"10.1007/s00299-025-03536-4","DOIUrl":"https://doi.org/10.1007/s00299-025-03536-4","url":null,"abstract":"<p><strong>Key message: </strong>Simulated animal feeding alters endogenous phytohormone levels and gene expression within related metabolic pathways, thereby regulating the growth, development, and defense mechanisms of Leymus chinensis. Leymus chinensis (Trin.) Tzvel. is a vital forage species in grassland animal husbandry, serving as the primary food source for grazing herbivores. Unraveling the mechanisms underlying herbage responses to grazing enhances our understanding of plant-animal interactions and their coevolution. In this study, we simulated grazing by applying animal saliva to L. chinensis post-clipping and conducted transcriptomic and phytohormonal analyses. Our findings revealed that differentially expressed genes (DEGs) responding to clipping but reverting to control levels after saliva treatment were enriched in a limited number of metabolic pathways. In contrast, DEGs specifically responsive to animal saliva were significantly enriched in numerous pathways related to plant growth, development, and defense. Quantitative real-time PCR (qRT-PCR) validated the expression patterns of representative DEGs, confirming the reliability of the transcriptome-based analyses. Five classes of DEGs were annotated in the plant hormone signal transduction pathway, including Auxin (IAA), Abscisic acid (ABA), Salicylic acid (SA), Cytokinin (CTK), and Jasmonic acid (JA). Phytohormone quantification revealed significant changes in hormone levels upon saliva treatment, peaking at 6 h post-treatment, which may account for the surge in DEGs observed at this time point. These results deepen our understanding of how animal saliva influences the growth and defense of L. chinensis and may inform more effective grassland management practices.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"148"},"PeriodicalIF":5.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.","authors":"Zhi Zou, Xiaowen Fu, Na Li, Panyan Zheng, Ruiling Wang, Hongyan Liu, Jiaquan Huang, Yongguo Zhao","doi":"10.1007/s00299-025-03534-6","DOIUrl":"https://doi.org/10.1007/s00299-025-03534-6","url":null,"abstract":"<p><strong>Key message: </strong>A WRI-like gene (CeWRI3) homologous to AtWRI3/4 was isolated from oil-rich Cyperus esculentus tubers, which was shown to function in TAG accumulation through activating CeOLE2. In oilseeds, WRI1 has proven to be a master regulator in the transcriptional control of genes involved in fatty acid biosynthesis. By contrast, no evidence is available for WRI1 and its homologs in regulating genes associated with triacylglycerol (TAG) biosynthesis. In this study, we present the characterization of a WRINKLED (WRI)-like gene from tigernut (Cyperus esculentus L., Cyperaceae), a rare example accumulating high levels of TAGs in underground tubers. This gene was named CeWRI3 for its homology to AtWRI3/-4 in Arabidopsis thaliana. Subcellular localization analysis in Nicotiana benthamiana and transactivation assay in yeast revealed that CeWRI3 indeed functions as a transcription factor with nuclear localization and transcriptional activation activity. Further qRT-PCR analysis showed that CeWRI3 exhibits a constitutive expression pattern and its transcripts are positively correlated with TAG accumulation during tuber development. Moreover, transient overexpression of CeWRI3 in N. benthamiana leaves could significantly enhance the TAG content, implying its contribution to TAG accumulation in tigernut tubers. Correspondingly, yeast one-hybrid and dual-luciferase reporter assays revealed that CeWRI3 could activate CeOLE2, but not CeOLE5 and CeDGAT2b, another two key genes related to TAG biosynthesis. Our findings emphasize the potential application of CeWRI3 in improving oil production of vegetative tissues.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"146"},"PeriodicalIF":5.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Sodium nitroprusside modulates oxidative and nitrosative processes in Lycopersicum esculentum L. under drought stress.","authors":"Cengiz Kaya, Ferhat Uğurlar, Chandra Shekhar Seth","doi":"10.1007/s00299-025-03529-3","DOIUrl":"10.1007/s00299-025-03529-3","url":null,"abstract":"","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"145"},"PeriodicalIF":5.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plantago species are emerging model organisms for functional genomics and stress biology.","authors":"Hannah Levengood, Lillian Smith, Shelby Gillis, Yun Zhou, Cankui Zhang","doi":"10.1007/s00299-025-03530-w","DOIUrl":"10.1007/s00299-025-03530-w","url":null,"abstract":"<p><strong>Key message: </strong>Species in the Plantago genus are emerging model organisms to multiple research disciplines. The genus Plantago has long been recognized for its significance in various research fields, yet it remains underutilized as a model organism in scientific studies. Several Plantago species possess unique traits, including easily accessible vascular tissues, medicinal properties, gynodieocity, and remarkable adaptability to diverse environmental conditions. These characteristics position Plantago as a promising model for research in areas such as plant vascular biology, stress physiology, reproductive biology, ecology, and medicinal biochemistry. Recent advancements, including the development of genetic transformation systems, the availability of sequenced genomes, and the application of CRISPR-Cas9 technology, have significantly enhanced the capability of using Plantago as a model system. This review discusses the research potential of Plantago species, highlighting key historical discoveries and recent breakthroughs that demonstrate their value across multiple scientific disciplines.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"142"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A loss-of-function of ZmWRKY125 induced by CRISPR/Cas9 improves resistance against Fusarium verticillioides in maize kernels.","authors":"Letizia Ottaviani, Rozenn Lefeuvre, Emilie Montes, Thomas Widiez, Paola Giorni, Axel Mithöfer, Adriano Marocco, Alessandra Lanubile","doi":"10.1007/s00299-025-03544-4","DOIUrl":"https://doi.org/10.1007/s00299-025-03544-4","url":null,"abstract":"<p><strong>Key message: </strong>ZmWRKY125 negatively regulates maize resistance to Fusarium verticillioides infection through modulating phytohormone, ROS scavenging and secondary metabolite gene expression as well as jasmonic and abscisic acid biosynthetic pathway activity. Fusarium verticillioides causes heavy damage to maize growth and yield and is responsible for mycotoxin contamination. Despite its widespread occurrence, few resistant genes have been identified and functionally validated for their role in the defense mechanisms against this fungus in maize. WRKY transcription factors are known to be crucial in regulating the expression of defense-responsive genes towards pathogen attack. In this context, in our previous genome-wide association study one SNP in the gene ZmWRKY125 was found significantly associated with the responses to F. verticillioides infection in maize seedlings. Here, loss-of-function mutant lines of ZmWRKY125 were obtained by the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system. The zmwrky125 edited lines were phenotypically evaluated showing a decrease by about 5 and 4 times of Fusarium ear rot (FER) severity and fumonisin contamination, respectively, compared to the wild-type genotype. The transient expression of ZmWRKY125 in maize protoplasts confirmed a nuclear localization as expected for a transcription factor. RNA-seq analysis comparison using two zmwrky125 edited lines and the wild-type genotype highlighted an enhanced modulation of the jasmonic acid (JA) and abscisic acid (ABA) hormones, redox state, cell wall modification, and secondary metabolism-associated genes after fungal infection. Moreover, the increased expression of JA- and ABA-related genes correlated with a wider accumulation of these two phytohormones in the mutant background in contrast to wild-type. This data provided new information for understanding the function of ZmWRKY125, despite further field evaluations will be required for validation of the resistance against FER.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"144"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression analyses of sweet cherry AUX/IAAs and involvements of PavAUX/IAA9/11 in regulating fruitlet abscission.","authors":"Qiandong Hou, Guang Qiao, Chunqiong Shang, Luonan Shen, Kiu Zhou, Yi Min, Xiaopeng Wen","doi":"10.1007/s00299-025-03539-1","DOIUrl":"https://doi.org/10.1007/s00299-025-03539-1","url":null,"abstract":"<p><strong>Key message: </strong>The sweet cherry genome contains 25 AUX/IAA members, of which PavAUX/IAA9 and PavAUX/IAA11 negatively and positively regulate fruitlet abscission, respectively. The auxin/indole-3-acetic acid (AUX/IAA) gene family is plant-specific and plays various roles in growth and development. However, its function in sweet cherry fruitlet abscission remains unclear. This study identified 25 AUX/IAA members in the sweet cherry (Prunus avium L.) genome, which exhibit conserved gene structures and are subjected to purifying selection. The promoter regions of these genes contain numerous auxin response-related cis-regulatory elements. RNA-seq and RT-qPCR analyses revealed that several genes, including PavAUX/IAA9, were down-regulated, whereas PavAUX/IAA11 was up-regulated during the fruitlet abscission. Both PavAUX/IAA9 and PavAUX/IAA11 are nuclear-localized proteins, and their overexpression in Arabidopsis thaliana alters growth-related phenotypes, and delays or promotes petal abscission, respectively. Yeast two-hybrid (Y2H) and luciferase complementation imaging (LCI) assays demonstrated that PavAUX/IAA9 interacts with itself and with PavTMP87. Yeast one-hybrid and dual-luciferase reporter assays demonstrated that PavARF4 and PavWRKY40 interact with the promoters of PavAUX/IAA9 and PavAUX/IAA11, respectively. PavARF4 and PavWRKY40 lack transcriptional activation activity in yeast systems, suggesting that they function as transcriptional repressors. The regulation of AUX/IAAs expression by sweet cherry ARF and WRKY transcription factors, coupled with the interaction between AUX/IAA and transmembrane proteins (such as PavTMP87), suggests a complex auxin signaling pathway involved in fruitlet abscission in sweet cherry. This highlights the critical regulatory function of AUX/IAA proteins in plant organ abscission.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"143"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}