Plant Cell Reports最新文献

{"title":"Loss-of-function of VrMYB104 in Mungbean hampers growth and multiple adaptive responses under waterlogging stress.","authors":"Prashasti Pandey, Shivani Kashaudhan, Siddharth Maurya, Deepak Kumar","doi":"10.1007/s00299-025-03625-4","DOIUrl":"https://doi.org/10.1007/s00299-025-03625-4","url":null,"abstract":"<p><strong>Key message: </strong>VrMYB104, a R2R3 MYB transcription factor, plays a key role in mungbean's waterlogging stress tolerance by modulating oxidative, osmotic, and hypoxia responsive genes' expression. In tropical and subtropical areas, mungbean (Vigna radiata) is an essential legume crop; however, waterlogging stress poses a serious threat to its productivity. Plant stress responses are known to be regulated by R2R3 MYB transcription factors (TFs). Little is known about their functional roles in mungbean under waterlogging stress. In this study, we identified 70 R2R3 type MYB TFs, through a genome-wide identification in mungbean which were characterized in-silico using bioinformatic analyses, such as chromosomal distribution, promoter cis-element prediction, motif structure, and phylogeny. VrMYB104 was selected for functional validation under waterlogging stress based on promoter analysis and spatio-temporal studies. Loss-of-function analysis using virus-induced gene silencing (VIGS) revealed growth retardation and yield reduction in silenced plants. Biochemical assays under waterlogging stress showed decreased proline levels, reduced activities of antioxidant enzymes (SOD, APX, CAT), and elevated reactive oxygen species (ROS), indicating impaired oxidative and osmotic stress responses. Expression profiling further revealed upregulation of PDC and LDH and downregulation of ACS, suggesting that VrMYB104 coordinates fermentative metabolism and hormonal signaling under stress. Collectively, these results establish VrMYB104 as a critical regulator of multiple stress-response pathways and highlight its potential for developing waterlogging-tolerant mungbean cultivars.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 11","pages":"233"},"PeriodicalIF":4.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmbZIP38 interacts with ZmDnaJ to regulate maize seedling drought tolerance through modulating ABA signaling, stomatal closure and root growth under stress.","authors":"Anyi Dong, Nan Wang, Tinashe Zenda, Qian Yang, Yuan Zhong, Xiuzhen Zhai, Huijun Duan","doi":"10.1007/s00299-025-03612-9","DOIUrl":"https://doi.org/10.1007/s00299-025-03612-9","url":null,"abstract":"<p><strong>Key message: </strong>The drought stress-responsive transcription factor ZmbZIP38 interacts with ZmDnaJ to regulate maize drought tolerance through modulating ABA biosynthesis and signaling, stomatal closure and root growth under stress. Basic leucine zipper (bZIP) transcription factors (TFs) crucially regulate plant drought stress response. However, how bZIP TFs regulate maize drought tolerance remains elusive. Previously, we demonstrated that ZmDnaJ enhances maize drought tolerance by promoting ABA biosynthesis and stomatal closure. In this study, we have fished out ZmbZIP38 (by yeast one-hybrid analysis) as key interacting partner of ZmDnaJ, and elucidate its function in ZmDnaJ-mediated drought tolerance in maize. ZmDnaJ promoter analysis results showed that ZmbZIP38 directly targets ZmDnaJ by binding to ABRE motifs in the corresponding gene promoters. Overexpression of ZmbZIP38 significantly enhanced maize survival rate under drought stress, which was accompanied by expanded leaf area and higher stomatal closure. ZmbZIP38-overexpressing maize lines also showed enhanced ROS scavenging, reduced H₂O₂ and MDA accumulation, and up-regulated expression of antioxidant enzymes-associated genes. Moreover, ZmbZIP38-OE maize lines exhibited elevated ABA levels under drought stress, correlating with the up-regulated expression of ABA biosynthetic genes. Conversely, zmbzip38 knockdown mutants displayed reduced drought tolerance, evidenced by increased cell damage and decreased leaf area. Collectively, our findings demonstrate that ZmbZIP38 regulates maize seedling drought tolerance by modulating ABA biosynthesis and signaling, ROS scavenging and root growth, highlighting its potential role in abiotic stress response, and particularly enhancing maize drought tolerance.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 11","pages":"234"},"PeriodicalIF":4.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synchronization based on precise cell cycle progression identification to enhance tetraploid induction in '84 K' poplar.","authors":"Aoyu Ling, Yufei Xia, Yijia Jin, Shenxiu Jiang, Jianghai Shu, Kang Du, Pingdong Zhang, Xiangyang Kang","doi":"10.1007/s00299-025-03615-6","DOIUrl":"https://doi.org/10.1007/s00299-025-03615-6","url":null,"abstract":"<p><strong>Key message: </strong>This research develops a novel method for improving tetraploid induction efficiency in vitro by precisely identifying key cell cycle phases and optimizing synchronization treatments in '84 K' poplar. Cell cycle asynchrony is a critical bottleneck in tetraploid in vitro induction efficiency. 5-Aminouracil (5-AU), a known cell cycle inhibitor, provides a strategy to enhance tetraploid induction efficiency. However, as 5-AU primarily targets S-phase cells and demonstrates cytotoxicity, improving tetraploid induction efficiency requires first accurately identifying the peak window of S-phase cell accumulation in the cultured tissue. Additionally, it is crucial to determine the appropriate concentration range of 5-AU to minimize toxicity to the cultured tissue. In this study, we developed a cell cycle synchronization strategy and evaluated its effect on tetraploid induction efficiency. Flow cytometry and EdU fluorescence labeling were used to track the cell cycle progression of '84 K' poplar (Populus alba × P. glandulosa) leaves during in vitro culture. Our results showed that S-phase activity peaked between days 2 and 3 of differentiation, while the G2/M phase began on day 4. This defined the optimal synchronization window as days 2 to 3. Comet assays were conducted to evaluate DNA damage induced by 5-AU. The results showed that DNA fragmentation increased significantly at concentrations exceeding 1.7 mM. In contrast, at concentrations below 1.3 mM, comet assays indicated relatively low DNA damage, while flow cytometry analysis revealed suboptimal synchronization efficiency. Consequently, 1.5 mM was identified as the optimal concentration for synchronization induction. By applying this synchronization strategy, the tetraploid induction rate of '84 K' poplar was increased to an average of 40.90%. Our results demonstrate that precise synchronization of the cell cycle, with minimal cytotoxicity, can significantly improve tetraploid induction efficiency.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 11","pages":"232"},"PeriodicalIF":4.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metabolic landscape of tomato roots during arbuscular mycorrhizal symbiosis reveals lipid-related metabolic rewiring.","authors":"Qian Ding, Xiang-Yun Tian, Wen-Shen Wu, Feng-Jia Yu, Zhu-Qing Shao, Zhen Zeng","doi":"10.1007/s00299-025-03621-8","DOIUrl":"https://doi.org/10.1007/s00299-025-03621-8","url":null,"abstract":"<p><strong>Key message: </strong>This study reveals lipid-related metabolic rewiring in tomato roots during arbuscular mycorrhizal symbiosis, identifying potential candidate lipids for fungal carbon transfer and signaling. Arbuscular mycorrhizal (AM) symbiosis induces substantial metabolic rearrangement in host plants to facilitate nutrient exchange and symbiotic efficiency. While previous metabolomic studies have characterized metabolite shifts in AM symbiosis, the lipid-related metabolic rewiring underlying nutrient exchange in host plant roots remains poorly resolved. Here, we investigated the metabolic response in tomato roots colonized by AM fungi. A total of 219 differentially accumulated metabolites (DAMs) were identified by the ultra-high-performance liquid chromatography-tandem mass spectrometry analysis, with lipids and lipid-like molecules representing the predominant classes. The most significantly upregulated metabolite was 2-(14,15-epoxyeicosatrienoyl) glycerol, a 2-monoacylglycerols (2-MAGs) mapped to arachidonic acid metabolism. This compound represents a C20-based epoxy fatty acid-derived 2-MAG, distinct from the C16:0 2-MAG induced by AM symbiosis in legumes, thereby implying the possibility of transferring diverse lipid substrates from different host plants to AM fungi. Concurrently, enhanced accumulation of dihomo-γ-linolenic acid (DGLA) and arachidonic acid (ARA) in AM fungi colonized roots underscored alterations of arachidonic acid metabolism and unsaturated fatty acid pathway. Gene set enrichment analysis based on the transcriptome data revealed significant transition of the glycerophospholipid metabolism pathway, primarily driven by multiple lysophosphatidylcholine (LPC) species that showed significant upregulation. Integrated transcriptomic and metabolomic analysis identified 31 overlapping KEGG pathways, emphasizing the importance of lipid and amino acid metabolism. In summary, our integrated analysis demonstrates that lipid-related metabolic reprogramming, represented by the induction of 2-MAGs and LPCs, is a feature of AM symbiosis that enables cross-kingdom nutrient exchange and host metabolic adaptation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"230"},"PeriodicalIF":4.5,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmAP2 enhances plant tolerance to aluminum toxicity and phosphorus deficiency in Arabidopsis.","authors":"Cheng Li, Pengxin Du, Lidan Wang, Xin Xu, Hongying Zhong, Yanbo Cheng, Tengxiang Lian, Lu Li, Qibin Ma","doi":"10.1007/s00299-025-03622-7","DOIUrl":"10.1007/s00299-025-03622-7","url":null,"abstract":"<p><strong>Key message: </strong>Overexpression of the GmAP2 gene regulates several aspects of gene expression in Arabidopsis, including acid-aluminum stress, low-phosphorus stress, ERF family transcription factors, ABA signaling, and genes related to lateral root development. The overexpression of GmAP2 can help plants mitigate the effects of aluminum toxicity and increase their tolerance to low-phosphorus stress. Ethylene response factors (ERFs) represent one of the largest transcription factor families in plants and are involved in regulating biotic and abiotic stresses, as well as growth and development in higher plants. In this study, the soybean GmAP2 gene, encoding the AP2/ERF transcription factor, is a downstream gene of GsMYB7, which enhances soybean tolerance to aluminum toxicity under acidic conditions, referred to as acid-aluminum stress. The expression pattern of GmAP2, which is constitutive and rich in roots, was upregulated under acid-aluminum and low-phosphorus stress. The resistance phenotypes of the wild-type and GmAP2-overexpressing lines were investigated by treatment with acid-aluminum plates and low-phosphorus plates, respectively. The results revealed significant differences between the GmAP2 transgenic lines and the wild type, and compared with the wild-type plants, the genetically modified plants presented significantly better physiological indicators, such as fresh weight, root length, and number of lateral roots. Under acid-aluminum stress, changes in weight, MDA content, and proline content in Arabidopsis thaliana were evaluated. The genes associated with these pathways, including genes related to the acid-aluminum response (AtALMT, AtALS3, AtSTOP1, and AtMATE), ERF family (AtERF6, AtERF15, AtERF5, and AtERF109), and/or ABA response (AtABI5, AtRD29A, AtRAP2.6, AtABI1, and AtABI2), are significantly regulated. In the context of low-phosphorus stress, a comprehensive analysis of the regulatory landscape revealed significant alterations in genes involved in these pathways, including low phosphorus-responsive genes (AtPHT1; 1, AtPHO1, AtPHR1, and AtMGD2), ERF family genes (AtERF1, AtERF6, AtERF13, and AtERF109), and/or genes associated with lateral root development (AtKCS16). These regulatory changes occurred in response to low-phosphorus stress. These results provide a basis for breeding soybean varieties that can increase their tolerance to the stresses of aluminum toxicity and low phosphorus.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"231"},"PeriodicalIF":4.5,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12496291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145228606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evolution of the bZIP family in allotetraploid Gossypium and GhbZIP017 function under drought stress.","authors":"Fangting Ye, Jing He, Zhaowei Li, Huanyu Wu, Ziyou Zhang, Dan Fu, Yanchun Li, Jinwen Huang, Changlan Zhu, Kai Fan","doi":"10.1007/s00299-025-03605-8","DOIUrl":"https://doi.org/10.1007/s00299-025-03605-8","url":null,"abstract":"<p><strong>Key message: </strong>203 bZIP members were identified in each G. barbadence and G. hirsutum and GhbZIP017 from the A subfamily was a novel drought-related member in cotton. The basic leucine zipper (bZIP) transcription factors are widely distributed in plants, but the evolutionary history is still unclear in allotetraploid Gossypium barbadense and Gossypium hirsutum. In this study, 203 bZIP members were identified in each G. barbadense and G. hirsutum. The bZIP members could be further divided into 13 subfamilies. A total of 227 gene duplication events were discovered in two allotetraploid cotton species and mainly occurred in the cotton ancestor. Furthermore, the bZIP family had a conserved evolutionary history in two allotetraploid cotton species. Meanwhile, the bZIP members had tissue-specific expression levels. Moreover, through the RNA-seq analysis, GhbZIP017 from the A subfamily played an important role in drought stress response. GhbZIP017 was localized in the nucleus and acted as a transcriptional repressor. The expression levels of GhbZIP017 in leaves and roots could be highly induced by drought stress. GhbZIP017 overexpression in Arabidopsis could enhance drought tolerance with a higher survival rate, lower membrane ion leakage, higher SOD and POD activity, lower MDA content, and higher expression levels of some drought-related genes. Overall, these results could help us uncover the evolutionary history of the bZIP members in cotton and provide a candidate gene GhbZIP017 for drought breeding in G. hirsutum.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"229"},"PeriodicalIF":4.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the dual resistance pathways to mesosulfuron-methyl in Lolium multiflorum: target-site and non-target-site insights.","authors":"Pei Zhang, Xinyang Yuan, Yuqing Liu, Junchuan Song, Runqiang Liu, Hongliang Wang","doi":"10.1007/s00299-025-03620-9","DOIUrl":"https://doi.org/10.1007/s00299-025-03620-9","url":null,"abstract":"<p><strong>Key message: </strong>Lolium multiflorum exhibits resistance to mesosulfuron-methyl through ALS mutations; HZ2 population also shows metabolic resistance through P450 pathways. Lolium multiflorum L., is a weed that frequently appears in wheat fields and is recognized for its strong competitive nature, where it can cause significant damage to grain production. The weeds of L. multiflorum in the wheat fields may have developed resistance to mesosulfuron-methyl. This study explored the response of L. multiflorum populations in certain areas of Henan Province, China, to mesosulfuron-methyl. The study found that, compared to the HX1 sensitive population, the HZ1 and HZ2 populations showed resistance to mesosulfuron-methyl in the full dose-response test, with resistance ratios of 12.38- and 24.19-fold, respectively. Genetic sequencing revealed novel mutations at the Pro-197-Thr and Asp-376-Glu residues of the ALS gene in both resistant populations. A critical finding was the divergent resistance mechanisms between the geographically close populations, with HZ1 resistance solely conferred by target-site mutations and HZ2 exhibiting multiple resistance driven by both target-site mutations and enhanced metabolism mediated by cytochrome P450 monooxygenases. This was conclusively demonstrated by applying the P450 inhibitors malathion and PBO, which reversed resistance in HZ2 by 66.77% and 70.53%, respectively. Furthermore, both resistant populations showed heightened sensitivity to isoproturon, suggesting a potential management strategy. Molecular docking simulations corroborated that the identified mutations reduce herbicide binding affinity. Our findings provide the first evidence of concurrent target-site and non-target-site resistance to mesosulfuron-methyl in Chinese L. multiflorum, offering crucial insights for diagnosing and managing herbicide resistance.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"228"},"PeriodicalIF":4.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145200579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"G1-mediated OsMADS1 repression regulates sterile lemma development in rice.","authors":"Xuemei Qin, Jinliang Sun, Ru Li, Weijian Cen, Rongbai Li, Jijing Luo","doi":"10.1007/s00299-025-03619-2","DOIUrl":"https://doi.org/10.1007/s00299-025-03619-2","url":null,"abstract":"<p><strong>Key message: </strong>This study shows that transcription factor G1 represses the floral development regulator OsMADS1 by binding to the CArG-box and YACTGTW motifs in OsMADS1 promoter to specify sterile lemma development in rice. In addition, we first demonstrate that G1 physically interacted with the OsMADS1 K-box domain, which enhances G1-mediated repression of OsMADS1. Sterile lemma is a unique organ formation during the development of rice spikelet, and the regulatory mechanism underlying its development remains poorly understood. Previous studies showed that transcription factor G1 (Os07g0139300) plays a critical role in sterile lemma development. Here, we further reveal that G1 transcriptionally represses the expression of OsMADS1, a key gene regulating floral organ development in rice, by directly binding to the CArG box and YACTGTW motifs in its promoter. Importantly, we first demonstrate that G1 specifically targets the K-box domain of OsMADS1 to establish physical interaction, and the interaction significantly enhances G1-mediated transcriptional repression of OsMADS1 expression. The feedback regulatory loop formed between G1 and OsMADS1 provides a key clue for further elucidating the molecular mechanisms underlying sterile lemma development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"227"},"PeriodicalIF":4.5,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soybean GmWRKY44 transcription factor activates SOC1 and LFY to promote flowering in Arabidopsis thaliana.","authors":"Youju Huang, Cuijing Pang, Yongbo Yu, Shixu Sun, Musen Lin, Hongbing Yang, Yanchong Yu","doi":"10.1007/s00299-025-03616-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03616-5","url":null,"abstract":"<p><strong>Key message: </strong>Soybean GmWRKY44 can directly activate the expressions of SOC1 and LFY to promote flowering in Arabidopsis. WRKY transcription factors (TFs) play pivotal roles in regulating plant flowering; however, the molecular mechanism underlying flowering regulation by soybean (Glycine max) WRKY TFs remains elusive. In this study, we isolated GmWRKY44, a nuclear-localized Group IIc WRKY member exhibiting transcriptional activation capacity. GmWRKY44 displayed spatiotemporal specificity, with peak expression in 30 d post-germination stems. GUS staining showed that GmWRKY44 expressed in various tissues, such as roots, stems, leaves, sepals, stigmas, filaments, siliques and seedlings. GmWRKY44 promoter harbored 92 cis-elements associated with phytohormone responses, light signaling, abiotic stress adaptation and developmental regulation. Furthermore, the overexpression of GmWRKY44 in Arabidopsis led to an early-flowering phenotype, as evidenced by the significant upregulation of flowering activators SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), LEAFY (LFY), APETALA1 (AP1) and downregulation of the flowering repressor FLOWERING LOCUS C (FLC). Subsequent analyses, including Y1H, EMSA, and LUC assays, provided convincing evidences that GmWRKY44 directly bound to the promoters of SOC1 and LFY, thereby elevating their expression. Genetic complementation assays further revealed that OE44-1 soc1-2 and OE44-1 lfy-2 hybrids exhibited a later flowering time than OE44-1 plants, indicating that the loss of SOC1 or LFY genetically arrested the early-flowering of OE44-1. In summary, this study revealed that GmWRKY44 promoted flowering in Arabidopsis by directly upregulating SOC1 and LFY, thus addressing a critical knowledge gap in the molecular regulation of soybean WRKYs on flowering time and offering a novel candidate gene for optimizing flowering time to enhance soybean yield across diverse agroecological zones.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"226"},"PeriodicalIF":4.5,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145177861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial GPATs protect Arabidopsis from water stress by impacting cuticle and secondary cell wall development.","authors":"Qianru Jia, Yang Bai, Shujuan Zhang, Li Song, Hongmei Zhang, Wei Zhang, Qiong Wang, Xiaoqing Liu, Xin Chen, Qun Zhang, Huatao Chen","doi":"10.1007/s00299-025-03608-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03608-5","url":null,"abstract":"<p><strong>Key message: </strong>Mt-GPAT1/2 are involved in cutin biosynthesis and negatively regulate SCW formation by modulating the lignin and cellulose synthesis genes. Alterations in cuticle and SCW lead to plant water imbalance. Trichomes and cuticles are critical epidermal adaptations that serve protective roles in plants. The cuticle functions as a barrier, allowing for controlled interactions between the plant and its environment. Cutin synthesis is crucial for plants to withstand various external stresses. In this study, we report on the Arabidopsis mutant gpat1 gpat2, which exhibits a highly permeable cuticle and defects in trichome development. Mutation of GPAT1 and GPAT2 resulted in a reduction of cutin monomer. In gpat1 gpat2, the structure of the cuticular layer of the cell wall is notably altered. Additionally, GPAT1 and GPAT2 are found to downregulate the expression of genes involved in lignin and cellulose biosynthesis, which are related to secondary cell wall (SCW) formation. The dysfunction of GPAT1 and GPAT2 disrupted the water balance of the plant. Our findings reveal a network where mitochondrial GPAT1 and GPAT2 play roles in maintaining water balance by participating in both Arabidopsis cutin synthesis and SCW formation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"225"},"PeriodicalIF":4.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}