Plant Cell Reports最新文献

{"title":"Functional divergence of CAD-like family genes in Saccharum complex under biotic and abiotic stress.","authors":"Zhu-Qing Wang, Sheng-Ren Sun, Hao-Yu Wang, Zheng-Wang Bi, Hai-Long Chang, Shuo-Hang Zhang, Jun-Lv Chen, Yuan-Xia Qin, Jian-Tao Wu, Wei Zhang, Huan-Ying Xu, San-Ji Gao, Qin-Nan Wang","doi":"10.1007/s00299-025-03472-3","DOIUrl":"https://doi.org/10.1007/s00299-025-03472-3","url":null,"abstract":"<p><strong>Key message: </strong>A total of 54 genes of membrane attack complex/perforin (MACPF) superfamily were identified in Saccharum complex and function divergence among SsaCAD-like genes were present in plant against stressors. The membrane attack complex/perforin (MACPF) superfamily belongs to pore-forming proteins involving in innate and adaptive immunity in eukaryotes. The constitutively activated cell death (CAD) proteins contained the MACPF domain participate in plant defense responses under adverse conditions. However, the characteristics and functions of CAD-like genes in sugarcane are still poorly understood. In this study, 54 CAD-like genes were identified in three genomes from Saccharum complex including two clones (Np-X and AP85-441) of Saccharum spontaneum and a clone (Yunnan2009-3) of Erianthus rufipilus. All CAD-like genes were categorized into five phylogenetic groups (I-V). Various cis-acting elements related to stress responses, such as phytohormone response elements, were found in promoter regions. Transcriptome and RT-qPCR analysis demonstrated these genes possessing diverse expression profiles. The SsaCAD1-like1 gene was upregulated in sugarcane cultivars after cold treatment and infection by Xanthomonas albilineans (Xa) causing leaf scald. Meanwhile, this gene was downregulated under drought and ABA treatments but was upregulated and then downregulated across time-points of SA treatment. The SsaCAD4-like1 gene was downregulated under five abiotic stressors. Expression levels of two alleles (SsaCAD2-like1/2) were significantly decreased under all abiotic stressors except for salinity treatment. Similar expression patterns of three alleles (SsaCAD3-like1/2/4) were found under abiotic stress. The SsaCAD1-like1 exhibited a negative role but SsaCAD3-like2/4 acted as positive roles in transgenic Arabidopsis lines against bacterial pathogen infection. Our results provide novel gene resources for developing disease-resistant cultivars in sugarcane.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"85"},"PeriodicalIF":5.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid induced by Bacillus megaterium causing systemic resistance against collar rot in Capsicum chinense.","authors":"Priyanka Gogoi, Bhaben Sharmah, Prasenjit Manna, Pinku Gogoi, Gakul Baishya, Ratul Saikia","doi":"10.1007/s00299-025-03470-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03470-5","url":null,"abstract":"<p><strong>Key message: </strong>Our findings suggest that the phytohormone salicylic acid, stimulated by Bacillus megaterium JPR68, plays a role in mitigating collar rot disease of Bhut Jolokia (Capsicum chinense Jacq.) Salicylic acid (SA) is a phytohormone that stimulates the plants immune response against various diseases. However, its function as a signaling molecule, particularly in relation to systemic acquired resistance (SAR) and induced systemic resistance (ISR), is still unclear. In this study, Bacillus megaterium JPR68 (BmJPR68) enhances the ISR of Capsicum chinense Jacq., resulting in elevated levels of SA within the plants. SA effectively inhibited the mycelial growth of Rhizoctonia solani and significantly reduced the necrosis, chlorosis, and collar rot in plants. The in vitro investigation revealed that the mycelial growth declined with increasing concentrations of SA and was completely inhibited at a concentration of 15 mM. The pathogenicity assay showed that leaves and fruits treated with SA impeded hyphal development and significantly retarded the growth of R. solani. In split root techniques, more SA was accumulated in the root tissues at the bacterized site compared to the non-bacterized side, although this accumulation reduced after 45 days. Additionally, the production of reactive oxygen species (ROS) was significantly diminished in plants treated with BmJPR68. SA production was assessed in both BmJPR68 and induced plants, indicating that the bacterial strain produced more SA compared to the induced plants. Fourier-transform infrared (FT-IR) analysis confirmed the presence of functional groups like O-H, N-H, S = O, C = C, C-N, and carboxylic/amine. The isoform of pathogenesis-related (PR) proteins was detected in the induced plants. This study provided valuable insights into SA induction using BmJPR68 to manage fungal disease in Capsicum chinense Jacq. during induced systemic resistance.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"86"},"PeriodicalIF":5.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of ascorbate peroxidase and functional characterization of SpAPX249b and SpAPX285c for salt tolerance in Sesuvium portulacastrum L.","authors":"Houli Zhou, Yuxin Li, Boxuan Yuan, Qinqin Nie, Zhaozhen Xiang, Lixia He, Yongfei Wang, Zhanchao Yang, Juanying Wang, Shugang Hui, Xuchu Wang","doi":"10.1007/s00299-025-03466-1","DOIUrl":"https://doi.org/10.1007/s00299-025-03466-1","url":null,"abstract":"<p><strong>Key message: </strong>We have identified 33 SpAPXs from S. portulacastrum genome and found SpAPX249b and SpAPX285c are important for halophyte salt tolerance. Ascorbate peroxidase (APX) is a vital antioxidant enzyme, involved in plant development and stress response by scavenging excessive reactive oxygen species (ROS). APX genes have been characterized in many plant species. However, their role in Sesuvium portulacastrum L. has not yet to be fully investigated. Here, we identified 33 SpAPXs from its genome and divided them into five subgroups across the 16 chromosomes. Cis-element analysis of their promoters indicated that all the detected SpAPXs showed potential roles in response to biotic and abiotic stresses as well as phytohormone effects on the plant growth and development. Transcriptomic data of the different tissues revealed that 9 SpAPX genes were specifically expressed in root and 13 ones were specifically expressed in leaves, with SpAPX249b prominently expressed in root and SpAPX285c in leaves. Moreover, quantitative real-time PCR analysis revealed that both SpAPX249b and SpAPX285c genes expressed only after NaCl application and were sharply induced in the high concentration of NaCl treatments. Our findings suggested that SpAPX249b and SpAPX285c may associate with plant salt tolerance and can serve as valuable genes for enhancing salt tolerance in other plants. By introducing these genes into other plants, it is possible to develop new varieties of salt-tolerant crops, thereby expanding the utilization of saline-alkali land and increasing agricultural productivity. In coastal saline-alkali wetlands, this halophyte can thrive in large numbers due to its inherent salt-tolerant genes, contributing to the restoration of polluted or ecologically degraded coastal saline-alkali wetlands.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"83"},"PeriodicalIF":5.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of regulating the growth and development of tomato seedlings by the crosstalk between JA and TOR signaling.","authors":"Hongyun Xing, Yipeng Bai, Qi Ding, Haoran Wang, Guorui Gao, Ziqiang Hu, Yang Yu, Haiyan Fan, Xiangnan Meng, Na Cui","doi":"10.1007/s00299-025-03476-z","DOIUrl":"https://doi.org/10.1007/s00299-025-03476-z","url":null,"abstract":"<p><strong>Key message: </strong>Transcription factors MYB, WRKY, bHLH, bZIP and NAC were identified as key candidate genes for JA and TOR regulation of tomato seedling growth and development. Jasmonic acid (JA) and Target of Rapamycin (TOR) signaling pathways interact to regulate plant growth, development, and stress responses. In this study, transcriptomic and weighted gene co-expression network analysis (WGCNA) were conducted on tomato wild-type (WT) and spr2 mutant lines treated with the TOR inhibitor RAP and activator MHY1485. We identified key roles of MAPK kinase and ethylene signaling in mediating JA-TOR interaction. Core transcription factors, including MYB, WRKY, bHLH, bZIP, and NAC, were highlighted as central regulators within the interaction between JA and TOR signaling network. These findings advance our understanding of how JA and TOR signaling coordinate plant growth and stress adaptation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"82"},"PeriodicalIF":5.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PpBOR1 is critical for the excess borate tolerance of Physcomitrium patens.","authors":"Ishfaq Ahmad, Xuejia Sun, Yangyang Yu, Fangni Jia, Yizuo Li, Qiang Lv, Yong Hu, Fang Bao, Yikun He","doi":"10.1007/s00299-025-03473-2","DOIUrl":"https://doi.org/10.1007/s00299-025-03473-2","url":null,"abstract":"<p><strong>Key message: </strong>Functional analysis of BORs in Physcomitrium patens indicates that both PpBOR1 and PpBOR2 possess boron efflux transporter activity, and PpBOR1 is essential for the plant's tolerance to excessive boron stress. Boron (B), an essential plant micronutrient, is crucial for achieving optimal agricultural yield. Although the function of the BOR family proteins as borate efflux transporters has been established in tracheophytes, the role of their counterparts in non-vascular plants has not been thoroughly investigated. Our phylogenetic analysis reveals that bryophyte BOR proteins originated from the basal bryophytes Takakia and Sphagnum, and can be classified into two subclasses. There are two BOR homologs in P. patens: PpBOR1 and PpBOR2, which belong to different subclades. The PpBOR1 and PpBOR2 genes are predominantly expressed in gametophores, with PpBOR1 exhibiting significantly higher expression levels than PpBOR2. Both proteins localize at the plasma membrane and can export borate from yeast cells. Disruption of PpBOR2 expression does not affect plant growth under normal conditions. However, PpBOR1-knockout gametophores exhibit stunted growth under excess boron conditions, whereas PpBOR1-overexpressing plants show enhanced tolerance compared to wild-type plants. In summary, our research suggests that BOR homologous proteins in P. patens have borate efflux activities similar to those of the BOR family members in angiosperms. PpBOR1 is critical in conferring tolerance to excessive boron stress in P. patens.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"81"},"PeriodicalIF":5.3,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An engineered PD1-Fc fusion produced in N. benthamiana plants efficiently blocks PD1/PDL1 interaction.","authors":"Shiva Izadi, Rafaela Abrantes, Simon Gumpelmair, Vinny Kunnummel, Henrique O Duarte, Peter Steinberger, Celso A Reis, Alexandra Castilho","doi":"10.1007/s00299-025-03475-0","DOIUrl":"10.1007/s00299-025-03475-0","url":null,"abstract":"<p><strong>Key message: </strong>Plant-made PD1-Fc fusions engineered for optimized glycosylation and Fc-receptor engagement are highly efficient in blocking PD1/PDL1 interactions and can be cost-effective alternatives to antibody-based immune checkpoint inhibitors. Immune checkpoint inhibitors (ICIs) are antibodies to receptors that have pivotal roles during T-cell activation processes. The programmed cell death 1 (PD1) can be regarded as the primary immune checkpoint and antibodies targeting PD1 or its ligand PDL1 have revolutionized immunotherapy of cancer. However, the majority of patients fail to respond, and treatment resistance as well as immune-related adverse events are commonly associated with this therapy. Alternatives to antibody-based ICIs targeting the PD1 pathway may bear the potential to overcome some of these shortcomings. Here, we have used a plant expression platform based on the tobacco relative Nicotiana benthamiana to generate immunoglobulin fusion proteins harboring the wild type or an affinity-enhanced PD1 ectodomain. We have exploited the versatility of our system to generate variants that differed regarding their glycosylation profile as well as their capability to engage Fc-receptors. Unlike its wild-type counterpart, the affinity-enhanced versions showed strongly augmented capabilities to engage PDL1 in both protein- and cell-based assays. Moreover, in contrast with clinical antibodies, their binding is not affected by the glycosylation status of PDL1. Importantly, we could demonstrate that the plant-made PD1 fusion proteins are highly efficient in blocking inhibitory PD1 signaling in a T cell reporter assay. Taken together, our study highlights the utility of our plant-based protein expression platform to generate biologics with therapeutic potential. Targeting PDL1 with plant derived affinity-enhanced PD1 immunoglobulin fusion proteins may reduce overstimulation associated with antibody-based therapies while retaining favorable features of ICIs such as long serum half-life.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"80"},"PeriodicalIF":5.3,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering scutellarin biosynthesis in Artemisia annua.","authors":"Dan Li, Xingyue Wu, Xinyu Qi, Zeying Zhang, Lingjiang Zeng, Xiaoqiang Liu, Fangyuan Zhang, Xiaozhong Lan, Min Chen, Mohammad Mahmoud Nagdy, Zhihua Liao","doi":"10.1007/s00299-025-03471-4","DOIUrl":"https://doi.org/10.1007/s00299-025-03471-4","url":null,"abstract":"<p><strong>Key message: </strong>Heterologous synthesis of scutellarin was successfully achieved in Artemisia annua by supplementing missing enzymes and optimizing flavone 6 hydroxylase in the biosynthetic pathway after identifying two crucial precursors in wild type plants. Artemisia annua, a plant renowned for its antimalarial properties, harbors a diverse array of terpenoids, phenols and other natural products along with their respective precursors. Engineering A. annua plants through synthetic biology holds significant promise to produce drugs in scarcity. Herein, we identified two essential precursors of scutellarin, an ingredient known for its remarkable therapeutic efficacy in treating cerebrovascular and cardiovascular diseases, within wild-type A. annua plants. To facilitate the heterologous synthesis of this bioactive compound in A. annua, we co-expressed three key genes derived from the original host, Erigeron breviscapus: the flavone synthase II gene (EbFSII), the flavonoid-7-O-glucuronosyltransferase gene (EbF7GAT), and the flavone-6-hydroxylase gene (EbF6H). These engineered plants successfully synthesized scutellarin at levels ranging from 0.18 to 0.24 mg/g DW. Furthermore, the introduction of the flavone-6-hydroxylase gene from Scutellaria baicalensis (SbF6H), which demonstrated superior catalytic activity, significantly increased scutellarin generation, achieving concentrations of up to 0.64 mg/g DW. Notably, the insertion of these exogenous genes did not negatively affect the synthesis of artemisinin and its derivatives in A. annua. These findings suggest that A. annua offers a formidable foundation for the biosynthesis of scutellarin. Additionally, the results imply that enhancing the activity of critical enzymes boosts the yield of the valuable terminal products.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"79"},"PeriodicalIF":5.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotype and transcriptome analysis identify the key genes controlling seed size and oil accumulation in oil-Camellia.","authors":"Linxiu Liu, Yu Sheng, Yunbin Zhang, Xinru Xie, Juanjuan Chen, Jingfei Wang, Huanhuan Pan, Hu Huang, Xun Cao, Jing Xu, Renying Zhuo, Xiaohua Yao","doi":"10.1007/s00299-025-03454-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03454-5","url":null,"abstract":"<p><strong>Key message: </strong>Phenotypic analysis of an F1 oil-Camellia population, combined with transcriptome sequencing of its parental lines, identifies pivotal genes controlling seed size and oil accumulation. Oil - Camellia is a major oil-producing tree, known for its high nutritional value and health benefits. Improving seed size and oil content is the key breeding objective, governed by intricate genetic networks. However, the molecular mechanism underlying these traits in oil-Camellia has been rarely reported. In this study, an F₁ population was developed from two parental genotypes, CL4 (hexaploid with high oil content and large seed size) and XG (tetraploid with low oil content and small seed size), which exhibited significant variation in yield-related traits. Ploidy analysis of progenies of F₁ population showed that most individuals were tetraploids and hexaploids, with a smaller number of diploids and octoploids present. Additionally, the analysis identified progeny individuals exhibiting both large seed and high oil content although no clear correlation with ploidy was observed. Comparative RNA sequencing (RNA seq) of developing seeds at four developmental stages revealed dynamic expression patterns, associated with seed size and oil content in the two parents. Co-expression regulatory network and differentially expressed genes of fatty acid biosynthesis pathway indicated that genes, such as Oleosin5 and ACCase α-subunit, displayed central roles in controlling seed size and oil content with notable expression peaks in S3 and S4. Additionally, the ABA signaling pathway, along with expansin proteins and transcription factors, showed co-expression with these genes, suggesting a regulatory pathway centered around Oleosin5 and ACCase α-subunit. This study identifies essential genes linked to oil seed yield traits, enhancing the understanding of oil-Camellia's molecular breeding targets.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"78"},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the regulation mechanism of drought tolerance in wheat.","authors":"Tengteng Zhang, Ying Zhang, Yi Ding, Yufeng Yang, Dan Zhao, Huiqiang Wang, Yifan Ye, Haojia Shi, Bowen Yuan, Zizheng Liang, Yulu Guo, Yue Cui, Xigang Liu, Hao Zhang","doi":"10.1007/s00299-025-03465-2","DOIUrl":"https://doi.org/10.1007/s00299-025-03465-2","url":null,"abstract":"<p><p>Wheat (Triticum aestivum L.) is one of the most important crops in arid and semi-arid areas of the world, and its sustainable and efficient production is essential for ensuring food security in China and globally. However, with the global climate change, wheat production is increasingly endangered by abiotic stress, and drought stress has become the main abiotic stress factor restricting wheat production efficiently. Therefore, investigating drought resistance genes and elucidating the mechanisms underlying drought resistance regulation is crucial for the genetic enhancement of drought resistance and the development of new drought-resistant wheat varieties. This paper reviews the majority of research conducted on wheat drought resistance over the past five years, focusing on aspects, such as transcriptional regulation, protein post-translational modifications, and other regulatory mechanisms related to drought resistance in wheat. Additionally, this paper discusses future directions for the genetic improvement of drought resistance and the breeding of new drought-resistant wheat varieties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"77"},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome reveals Gafmt-1 and Gadlc-1-5 play positive roles in cotton resistance to Verticillium wilt.","authors":"Jiale Chen, Susu Liu, Guoli Feng, Jianbo Gao, Ningshan Wang, Nijiang Ai, Baoliang Zhou","doi":"10.1007/s00299-025-03462-5","DOIUrl":"https://doi.org/10.1007/s00299-025-03462-5","url":null,"abstract":"<p><strong>Key message: </strong>Both Gafmt-1 and Gadlc-1-5 from Gossypium arboreum respond to Verticillium dahilae infection in Gossypium hirsutum and may play positive roles in Verticillium wilt resistance via the salicylic acid pathway. Verticillium wilt (VW) caused by Verticillium dahliae is one of the most destructive diseases affecting cotton production and quality worldwide. Numerous resistance genes against the disease from tetraploid cultivated cotton (2n = 4x = AADD = 52) have been cloned and functionally analyzed to attempt to develop resistant varieties. However, VW continues to pose a significant threat to global cotton production due to the lack of cost-effective resistance genes to balance resistance and yield. Resistance genes from diploid cotton species such as Gossypium arboreum (2n = 2x = AA = 26) remain largely untapped, and their functions are unknown. Here, a resistant G. hirsutum-G. arboreum introgression line, DM10781, was employed to mine new resistance genes against V. dahliae from the diploid cotton species. We performed time-course transcriptome analysis on the RNA-seq data at 0, 4, 12, 24, 48, and 96 h post-inoculation. Weighted gene co-expression network analysis showed that nine differentially expressed genes (DEGs) caused by disease resistance have been identified. Among them, seven genes were found on the introgression segments from G. arboreum and suffered from virus-induced gene silencing in DM10781. Out of them, two genes were further overexpressing in Arabidopsis. The results indicated the two genes of Gafmt-1 and Gadlc-1-5 played positive roles in both cotton and Arabidopsis. Our study demonstrates that G. arboreum has the resistance genes to VW and can be used in future disease-resistance breeding, providing insights into the resistance of Gafmt-1 and Gadlc-1-5 against VW in cotton.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"76"},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}