Plant Cell Reports最新文献

{"title":"Enhancing phytoextraction efficiency of king grass through foliar application of plant growth regulators under cadmium and chromium stress.","authors":"Muhammad Mohsin Altaf, Xuezhi Dong, Meng Wang, Xiaoyan Sun, Dong Li, Zhiqiang Zhu","doi":"10.1007/s00299-026-03834-5","DOIUrl":"https://doi.org/10.1007/s00299-026-03834-5","url":null,"abstract":"<p><strong>Key message: </strong>Plant growth regulators (IAA and MT) enhance phytoextraction of cadmium and chromium in king grass by boosting plant growth, photosynthesis, antioxidant activity, and upregulating key enzymes, leading to improved metal accumulation and soil remediation efficiency. Heavy metal contamination, particularly cadmium (Cd) and chromium (Cr), threatens global soil health and food security. This study evaluated the role of eight plant growth regulators (PGRs) including indole-3-acetic acid (IAA), melatonin (MT), gibberellin (GA3), abscisic acid (ABA), cytokinin (CKS), ethylene (ETH), strigolactones (SL), and salicylic acid (SA) in enhancing phytoextraction by king grass (Pennisetum purpureum × P. americanum) across low (LP) and high-pollution (HP) soils. PGRs (0.2 mg L^-1) were foliar-sprayed (5 mL pot^-1) on king grass planted in LP and HP soil after every 10 days starting 40 days post-transplantation in the greenhouse. The plants were harvested after 90 days of transplanting to assess the plant growth, photosynthesis, antioxidant enzyme activities and phytoremediation efficiency of king grass. Foliar PGR application increased biomass (IAA: 23.49% DW in LP soil; MT: 41% DW in HP soil) and photosynthetic pigments while reducing oxidative stress markers (MDA: 36.20%, H₂O₂: 30.92% with IAA). Antioxidant enzymes (SOD, CAT, APX) were upregulated by 20-40%, correlating with enhanced Cd/Cr accumulation (IAA: 121% Cd, 82.08% Cr in shoots; MT: 49.72% Cd in HP soil). Remediation efficiency peaked with IAA (10.30% Cd-RE, 17.49% Cr-RE in LP) and ABA (7.20% Cd-RE in HP), demonstrating soil-specific PGR optimization. These findings establish MT and IAA as keystones for sustainable phytoremediation, aligning with global soil health initiatives.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From haploid inducer to CMS donor: repurposing of CENH3 to create a CMS line in a single step in Brassica napus.","authors":"Mengyu Lei, Muhammad Zeeshan Mola Bakhsh, Xiaoyu Zhang, Dongli He, Cheng Dai, Chaozhi Ma, Jingxin Tu, Jinxiong Shen, Jing Wen, Tingdong Fu, Bin Yi","doi":"10.1007/s00299-026-03804-x","DOIUrl":"https://doi.org/10.1007/s00299-026-03804-x","url":null,"abstract":"<p><strong>Key message: </strong>Mutation in centromere histone H3 (CENH3) protein could induce a paternal haploid with maternal cytoplasm in rapeseed. By paternal haploid induction, a cytoplasmic male sterile line can be created in any genetic background within one breeding cycle. Hybrid development in rapeseed relies primarily on the three-line system, which includes a cytoplasmic male sterile (CMS) line. Conventionally, these CMS lines are developed through backcrossing, a process that requires several breeding cycles to complete. More recently, the doubled haploid technique has been employed in various crops to generate homozygous lines within a single breeding cycle. In the present study, we utilized a haploid induction (HI) strategy to produce fertile homozygous lines and CMS lines via paternal haploid induction. We have created single homozygous and double heterozygous mutants of the BnaCENH3 gene in the rapeseed cultivar ganA (hau-CMS) using CRISPR/Cas9 technique. Upon hybridization of CMS-HI line with wild type can successfully induced paternal haploids with maternal sterile cytoplasm. This system offers the ability to introduce sterile cytoplasm into any genetic background within a single generation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ubiquitination as a multi-layer regulatory network in legume-rhizobium symbiosis.","authors":"Ping Wu, Zhongming Zou, Zhen Wu, Yong Feng","doi":"10.1007/s00299-026-03840-7","DOIUrl":"https://doi.org/10.1007/s00299-026-03840-7","url":null,"abstract":"<p><p>Symbiotic nitrogen fixation (SNF) by legumes is essential for sustainable agriculture, providing plant-available nitrogen while reducing reliance on synthetic fertilizers. The establishment of legume-rhizobium symbiosis requires tightly regulated host signaling to coordinate rhizobia infection, nodule development, and nitrogen fixation, while preventing excessive colonization or immune activation. Accumulating evidence indicates that ubiquitination, mediated by E1, E2, E3 ubiquitin ligases and deubiquitinating enzymes, plays a central role in controlling multiple stages of this process. In this review, we summarize current knowledge on ubiquitination-mediated regulation of symbiotic nitrogen fixation, with a focus on early symbiotic signaling and nodule development. We highlight key E3 ligases that modulate Nod factor receptor homeostasis, receptor-associated kinases, transcription factors, and infection thread growth, and discuss how ubiquitination interfaces with nutrient and stress signaling pathways. Finally, we outline key knowledge gaps and discuss the potential of manipulating ubiquitination pathways to improve nodulation efficiency and nitrogen use efficiency in crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Over-expression transcription factor ERF1 promotes seed germination by enhancing mitochondrial function.","authors":"Changliang Chen, Yupeng Cao, Wenbo Kai, Yuexin Wu, Yanchun Yan, Wei Wu","doi":"10.1007/s00299-026-03838-1","DOIUrl":"https://doi.org/10.1007/s00299-026-03838-1","url":null,"abstract":"<p><strong>Key message: </strong>ERF1 modulates mitochondrial function to fine-tune ROS homeostasis and suppress ABA signaling, thereby facilitating seed germination. Seed germination represents a critical phase in the plant life cycle, and its proper regulation is crucial for optimizing crop productivity. Mitochondria are essential organelles that provide energy for seed germination in plants. However, how mitochondria fine-tune seed germination remains poorly understood. In this study, we demonstrated that ETHYLENE RESPONSE FACTOR 1 (ERF1), a key transcription factor in the canonical ethylene signaling pathway, promotes seed germination by enhancing mitochondrial function. Through the integration of gene expression, physiological and biochemical characterization, and germination analyses, we revealed that overexpression of ERF1 strengthened mitochondrial function, thereby reducing reactive oxygen species (ROS) accumulation and facilitating seed germination. Moreover, we demonstrated that overexpression of ERF1 repressed the ABA signaling pathway by inhibiting the mitochondrial retrograde signaling (MRS) pathway, thereby reducing ROS levels via downregulation of RBOHD expression and activity and enhancing sugar supply, which finally promoted seed germination. These results provide new insights into how ERF1 modulates mitochondrial function to fine-tune ROS homeostasis and suppress ABA signaling during seed germination. This work provides valuable mechanistic insights and lays the groundwork for future strategies aiming at improving seed germination in agricultural crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combatting vanadium toxicity in rapeseed (Brassica napus L.) via soil amendment and foliar application of calcium oxide nanoparticles: insights into physio-biochemical and antioxidant defense mechanisms.","authors":"Muhammad Mohsin Altaf, Afrah E Mohammed, Modhi O Alotaibi, Motirh Al-Mutairi, Jun Wang","doi":"10.1007/s00299-026-03835-4","DOIUrl":"https://doi.org/10.1007/s00299-026-03835-4","url":null,"abstract":"<p><strong>Key message: </strong>Calcium oxide nanoparticles (CaONPs) effectively mitigate vanadium-induced toxicity in rapeseed by enhancing plant growth, photosynthesis, antioxidant defense, and nutrient uptake while reducing vanadium accumulation. Among variety of heavy metals (HMs), vanadium (V) is an important and most ruinous metal found in the earth crust. Use of nanoparticles is emerged as an important strategy with promising discretions for the solution of HMs induced drastic effects on plants. This study intended to determine how calcium oxide nanoparticles (CaONPs) could alleviate V stress in rapeseed (Brassica napus L.) plants through modulation of physiological parameters, activation of antioxidant defense systems, and regulation of stress-responsive gene expression. A greenhouse experiment was performed and utilized spherical CaONPs and size 8-20 nm as foliar and soil application to mitigate the V toxicity in rapeseed plants. The three levels of CaONPs (10,20, and 30 mg L ^-1) were applied via foliar and soil application to the rapeseed plants grown under V polluted soil (40 mg kg ^-1 soil). The application of CaONPs (S-30/40 and F-30/40) significantly improved plant physiological attributes such as plant fresh weight (75.16% and 134.86%), dry weight (183.88% and 284.36%), and root length (49.64% and 101.59%) as compared to V-treated plants. Photosynthetic pigments and gas exchange attributes showed a notable improvement with the application of CaONPs (S-30/40 and F-30/40). Besides this, antioxidant enzymes activities such as SOD (68.62 - 95.43%), CAT (92.67 - 161.48%), POD (35.50 - 49.04%) and APX (52.08 - 91.14%) were increased with CaONPs (S-30/40-F-30/40) while decreased the reactive oxygen production in rapeseed plant grown in V-stressed plants. The CaONPs application also effectively improved nutrient uptake like N, P, K, Mn, Mg, and Ca and inhibited the V accumulation in root and shoot under V-polluted soil. The results imply that CaONPs use might play a major role in sustainable farming in promoting crop tolerance to HM conditions.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin enhances the cold tolerance of tomatoes by promoting ABA accumulation.","authors":"Guangzheng Wang, Qing Yang, Jianhua Dou, Junwen Wang, Zhongqi Tang, Jihua Yu","doi":"10.1007/s00299-026-03825-6","DOIUrl":"https://doi.org/10.1007/s00299-026-03825-6","url":null,"abstract":"<p><strong>Key message: </strong>SlSNAT1-dependent melatonin synthesis enhances tomato cold tolerance by promoting SlNCED1-mediated ABA accumulation, thereby strengthening antioxidant capacity, maintaining photosynthesis, and reducing ROS-induced membrane damage under cold stress. Cold stress limits greenhouse tomato growth by suppressing photosynthesis, promoting reactive oxygen species (ROS) accumulation, and disrupting membrane integrity, which ultimately reduces yield. Melatonin (MT) enhances plant stress tolerance, but its role in regulating cold adaptation in tomato remains unclear. Here, we used CRISPR/Cas9-generated SlSNAT1 mutants and VIGS-mediated SlNCED1-silenced plants to test how MT influences abscisic acid (ABA) biosynthesis and cold tolerance. The SlSNAT1 mutation markedly reduced endogenous MT, decreased the expression of ABA biosynthetic genes (SlNCED1/2), and increased the expression of ABA catabolic genes (SlCYP707A1/2), thereby weakening cold-induced ABA accumulation. Accordingly, the mutants showed higher membrane permeability and ROS levels, together with lower photosynthetic efficiency and reduced antioxidant enzyme activity under cold stress. SlNCED1 silencing further reduced ABA accumulation and antioxidant capacity. By contrast, exogenous MT partly restored ABA content, antioxidant enzyme activity, and photosynthetic performance, thereby alleviating cold injury. Correlation analysis showed that ABA content was positively associated with antioxidant activity, photosynthetic traits, and osmotic regulators, but negatively associated with ROS levels and membrane damage. MT synthesis mediated by SlSNAT1 promotes ABA accumulation, at least in part, by enhancing SlNCED1 expression. These findings clarify a mechanism underlying MT-ABA cross talk during cold stress and suggest a potential strategy to enhance cold tolerance in greenhouse tomato.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DcMYB5 transcription factor regulates the biosynthesis of diosgenin by directly repressing CYP90B gene expression in Dioscorea composita.","authors":"Yinxing Cao, Jiani Zhang, Shangjie Yu, Chunmei Zhong, Jun Xie","doi":"10.1007/s00299-026-03830-9","DOIUrl":"https://doi.org/10.1007/s00299-026-03830-9","url":null,"abstract":"<p><strong>Key message: </strong>DcMYB5 regulates diosgenin biosynthesis in Dioscorea composita by binding to the DcCYP90B promoter and repressing its transcription. Diosgenin is an important bioactive metabolite in Dioscorea with significant pharmaceutical and ecological value. However, the transcriptional regulatory mechanisms of its biosynthesis remain poorly understood. Here, we identified and characterized an SG5 subgroup R2R3-MYB transcription factor (TF) from Dioscorea composita, designated DcMYB5. Sequence and phylogenetic analyses identified DcMYB5 as a MYB5-type SG5 MYB TF with typical R2R3 conserved domains but lacking the C3 motif at the C-terminus. Functional gene silencing and overexpression experiments validated that DcMYB5 modulates the biosynthesis of diosgenin and its derivatives, along with tuber growth. Dual-luciferase and yeast one-hybrid assays further indicated that DcMYB5 binds to the DcCYP90B promoter and represses its transcription, thereby regulating diosgenin accumulation. Overall, this study provides new insights into how R2R3-MYB TFs coordinate steroidal metabolism with growth in D. composita and delivers an actionable molecular target for agricultural biotechnology to enhance high-value steroidal saponin production.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conserved germanicol synthase lineage and a single-residue switch controlling triterpene scaffold divergence in Panax.","authors":"Xue Wang, Jingyang Ding, Shiyan Yuan, Haiyan Li, Yongkang Zhang, Guisheng Xiang, Xianbin Deng, Zihan Yang, Shengchao Yang, Xiaobo Li, Guanghui Zhang","doi":"10.1007/s00299-026-03810-z","DOIUrl":"https://doi.org/10.1007/s00299-026-03810-z","url":null,"abstract":"<p><strong>Key message: </strong>A conserved germanicol synthase (GNS) lineage exists in Panax; a single methionine-to-asparagine switch at residue 728 controls triterpene scaffold divergence from β-amyrin to germanicol. Triterpenoid scaffold diversification in Panax is governed by oxidosqualene cyclases (OSCs); however, it remains unclear whether this genus has the ability to produce noncanonical pentacyclic skeletons. In this study, we functionally characterized a previously unrecognized germanicol synthase (PvOSC9) alongside its paralog, β-amyrin synthase (βAS), known as PvOSC8, from Panax vietnamensis var. fuscidiscus. Structural comparisons reveal a single residue 728 (Asn ↔ Met) that alters carbocation folding trajectories, thereby establishing a minimal molecular switch for scaffold identity. Notably, PvOSC9 is enriched in flowers and responds to jasmonate, suggesting a possible context-dependent role of PvOSC9 in floral tissues. Collectively, these findings broaden the triterpene scaffold repertoire in Panax and offer a mechanistically grounded framework for programmable triterpenoid biosynthesis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rice cysteine protease OsEP3A promotes seedling growth and seed development and contains a nitrogen-starvation-responsive sequence.","authors":"Yong Chou Lin, Shinn Jia Tzeng, Shin Lon Ho","doi":"10.1007/s00299-026-03826-5","DOIUrl":"https://doi.org/10.1007/s00299-026-03826-5","url":null,"abstract":"<p><strong>Key message: </strong>The cysteine protease OsEP3A plays a positive role in rice seedling growth and seed development. Its expression isinduced by nitrogen starvation in suspension-cultured cells. Promoter activity assays using GUS reporter constructs identified a 43-bp nitrogen-starvation-responsive sequence, providing a novel cis-regulatory target for enhancingnitrogen use efficiency. Nitrogen (N) is a critical macronutrient that influences plant growth, development, and productivity. This study characterizes the rice cysteine protease gene OsEP3A and its promoter to elucidate its role in N-mediated developmental and transcriptional regulation. Transgenic rice lines overexpressing (OsEP3A-Ox) or silenced (OsEP3A-Ri) for OsEP3A were generated to assess its physiological functions. Overexpression of OsEP3A significantly enhanced shoot and root growth, whereas RNAi-silenced plants exhibited reduced height, shorter roots, and smaller seeds compared to wild type, indicating that OsEP3A positively regulates seedling and seed development. Expression analyses revealed that OsEP3A transcription was strongly induced under N-deficient conditions and repressed by both inorganic (NH₄NO₃) and organic (glutamine, asparagine) N sources. Under N-limited hydroponic culture, OsEP3A-RNAi seedlings showed severely impaired growth, underscoring the gene's essential role in internal N remobilization during deficiency. Promoter-reporter analyses using OsEP3A::GUS lines demonstrated strong activation of the OsEP3A promoter under N starvation and repression upon N resupply, suggesting N-dependent transcriptional control. Deletion and insertion analyses of the OsEP3A promoter identified a 43-bp N-starvation-responsive sequence (NSRS; - 278 to - 236 bp) as necessary and sufficient for starvation-induced transcriptional activation. This NSRS represents a novel cis-regulatory element responsive to N deprivation. Overall, OsEP3A acts as an N-starvation-activated cysteine protease that facilitates N recycling and seedling vigor, providing new insight into N-responsive regulatory mechanisms in rice and offering a potential molecular target for improving N-use efficiency in cereal crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytomelatonin receptor PMTR1 is crucial for melatonin-mediated plant broad-spectrum disease resistance.","authors":"Xiao Cheng, Yinghao Liu, Yu Zheng, Guoyin Liu, H Shi","doi":"10.1007/s00299-026-03817-6","DOIUrl":"https://doi.org/10.1007/s00299-026-03817-6","url":null,"abstract":"<p><strong>Key message: </strong>PMTR1 is essential for melatonin-improved plant broad-spectrum disease resistance to bacterial pathogens and viruses. Melatonin plays vital roles in various plant stress responses, and the identification of phytomelatonin receptor 1 (PMTR1) in recent decades further extends the phytomelatonin signaling pathway. However, whether and how PMTR1 participates in plant broad-spectrum disease resistance has remained unclear. Here, we confirmed that exogenous melatonin enhanced disease resistance to bacterial blight and mosaic disease, indicating the crucial role of melatonin in cassava broad-spectrum disease resistance. In addition, the transcription of MePMTR1 was induced by Xanthomonas axonopodis pv. manihotis (Xam), common mosaic virus (CMV) and exogenous melatonin, suggesting that MePMTR1 may be involved in cassava immunity. Further investigation illustrated that MePMTR1 was a positive regulator in disease resistance to Xam and CMV, and it was necessary for melatonin-improved broad-spectrum disease resistance to the bacterial pathogen and the virus. Moreover, RNA-sequencing identified many differentially expressed genes (DEGs) in melatonin-improved disease resistance through MePMTR1, including calmodulin-like proteins (CMLs), calmodulin-binding proteins (CaMBPs), transcription factors WRKYs and MYBs, respiratory burst oxidase homolog protein C (RBOHC), heat shock protein 20, pathogenesis-related protein (PR10), etc. In total, this study provides new light on the theoretical foundation of melatonin in modulating broad-spectrum disease resistance in cassava, extending the understanding of the melatonin signaling pathway in plant immunity.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"45 5","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147779168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}