Plant Cell Reports最新文献

{"title":"Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.","authors":"Hiroki Asa, Chikako Kuwabara, Kenji Matsumoto, Ryo Shigeta, Takaaki Yamamoto, Yu Masuda, Tetsuya Yamada","doi":"10.1007/s00299-025-03433-w","DOIUrl":"10.1007/s00299-025-03433-w","url":null,"abstract":"<p><strong>Key message: </strong>We generated soybean mutants related to two ß-amyrin synthase genes using DNA-free site-directed mutagenesis system. Our results suggested that one of the genes is predominant in the soyasaponin biosynthesis. Soyasaponins, which are triterpenoid saponins contained in soybean [Glycine max (L.) Merril], are responsible for the astringent aftertaste of soyfood, and their complete elimination from soybean seeds is a key challenge in the development of cultivars with improved taste. While the loss of function in the ß-amyrin synthase genes (GmBAS1 and GmBAS2) has proven effective in reducing soyasaponin content in soybean seeds, the specific functional roles of these genes remain unclear. In this study, site-directed mutagenesis was performed on two GmBAS loci using a DNA-free clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system. A complex of sgRNA targeting sequences conserved in the two loci and Cas9 protein was introduced into the shoot apical meristems of soybean embryonic axes via bombardment. Cleaved amplified polymorphic sequences (CAPS) analysis conducted 1 month post-bombardment revealed that 138 seedlings out of 1,467 screened exhibited mutations at one or both GmBAS loci. CAPS and sequencing analysis in the subsequent generation identified a total of 16 plants with inheritable mutations ranging from one to ten nucleotides. High-performance liquid chromatography (HPLC) analysis showed that site-directed mutagenesis in the GmBAS1 locus resulted in the absence of soyasaponins in mature seeds, as well as in young roots, stems, and leaves. These findings demonstrate that GmBAS1 is the predominant ß-amyrin synthase gene in soybean plants. In addition, the DNA-free CRISPR/Cas9 system was shown to be highly efficient in inducing simultaneous mutagenesis at two target loci using a single gRNA.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"40"},"PeriodicalIF":5.3,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokinin negatively regulates tomato fruit ripening by influencing the ethylene pathway.","authors":"Mingjia Chen, Hui Zhang, Shanqi Cao, Mengying Song, Dexing Yin, Xi Wang, Manman Wei, Changhua Zhu, Na Yang, Lijun Gan","doi":"10.1007/s00299-025-03430-z","DOIUrl":"https://doi.org/10.1007/s00299-025-03430-z","url":null,"abstract":"<p><strong>Key message: </strong>Reducing endogenous CK levels accelerates fruit ripening in tomato by regulating ethylene biosynthesis and signalling pathway. Tomato is a typical climacteric fruit and is recognized as one of the most important horticultural crops globally. The ripening of tomato fruits is a complex process, highly regulated by phytohormones. Cytokinin (CK) is a hormone that primarily impacts the early development of fruit, however its influence on fruit ripening has not been thoroughly investigated. In this study, we used both wild-type Micro-Tom and transgenic tomato plants that overexpress AtCKX2, a CK degradation gene driven by the fruit-specific promoter Tfm7, to investigate the effect of CK on tomato fruit ripening. Our findings revealed that reducing endogenous CK levels in transgenic plants can accelerate the ripening process of tomato fruits. Premature activation of ethylene biosynthetic genes and ripening regulator genes was upregulated in CK-deficient fruits. Moreover, the application of exogenous ethylene inhibitors resulted in delayed fruit ripening in CK-deficient fruits. These results together suggest that CK plays a negative role in tomato fruit ripening by affecting the ethylene pathway.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"41"},"PeriodicalIF":5.3,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin improves the lead tolerance in Plantago ovata by modulating ROS homeostasis, phytohormone status and expression of stress-responsive genes.","authors":"Shreosi Chakraborty, Sarmistha Sen Raychaudhuri","doi":"10.1007/s00299-025-03424-x","DOIUrl":"https://doi.org/10.1007/s00299-025-03424-x","url":null,"abstract":"<p><strong>Key message: </strong>Melatonin increases Pb tolerance in P. ovata seedlings via the regulation of growth and stress-related phytohormones, ROS scavenging and genes responsible for melatonin synthesis, metal chelation, and stress defense. Lead (Pb) is a highly toxic heavy metal that accumulates in plants through soil and air contamination and impairs its plant growth and development. Because of its pharmaceutical importance, improvements in Plantago ovata yield against abiotic stresses are necessary. Melatonin (MEL) is a stress-alleviating biostimulator and our results showed a reduction in Pb induced phytotoxicity by enhancing plant growth attributes and balancing protective osmolytes. Pb-induced reactive oxygen species accumulation, including superoxide and peroxide free radicals and their mitigation through enzymatic antioxidants, was demonstrated in presence of MEL. Cell viability and Pb bioaccumulation were determined to understand the extent of cellular damage. Moreover, MEL increased secondary metabolite (flavonoids and anthocyanins) contents by 2-3-fold at the lowest Pb concentrations. Similar increases in the relative expression of genes (PoPAL and PoPPO), which are responsible for the production of non-enzymatic antioxidants, were observed. Notably, the upregulation of the PoCOMT gene up to 4-fold indicates increased melatonin production, as manifested in the phytomelatonin level. MEL supplementation also increased the auxin (IAA) level by 3-fold in the 100 µM Pb treatment group, while the abscisic acid (ABA) level decreased (1.4-fold) and the expression of PoMYB (a stress-related transcription factor) increased (up to 2.66-fold). Additionally, we found extreme downregulation (up to 18-fold) in the relative expression of PoMT 2 (a metal binding thiol compound) with melatonin treatment, which is otherwise upregulated (by 6-fold) during Pb stress. In the current study, these effects collectively revealed that MEL contribute to enhanced plant growth and Pb stress tolerance.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"39"},"PeriodicalIF":5.3,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the dual roles of sec-dependent effectors from Candidatus Liberibacter asiaticus in immunity of citrus plants.","authors":"Xue Li, Yue Zhou, Hang Chen, Zetian Guo, Jinlian Zhang, Wenli Chen","doi":"10.1007/s00299-024-03397-3","DOIUrl":"https://doi.org/10.1007/s00299-024-03397-3","url":null,"abstract":"<p><strong>Key message: </strong>The three SDEs of CLas were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of CLas and inhibited citrus immunity. Huanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus (CLas). CLas suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies. In this study, we focused on the roles of SDE4310, SDE4435 and SDE4955 in citrus. We found that the expression of SDE4310, SDE4435 and SDE4955 to increase with increasing citrus immune genes CsPR1, CsPR2, CsPR5, CsNPR1, CsRBOHD, CsMAP3K and CsBIK1, suggesting that the level of citrus immunity could be judged by the expression of SDE. To further explore the relationship between these three SDEs and citrus immunity, we established a transient expression system in citrus leaves, using gold nanoparticle-polyethyleneimine (AuNPs-PEI) to deliver recombinant plasmid containing SDE4310, SDE4435 or SDE4955 respectively into citrus leaves. Results showed that SDE4310, SDE4435 and SDE4955 were successfully expressed in citrus leaves using this transient expression system, and found that SDE4310, SDE4435 and SDE4955 could promote the CLas proliferation by decreasing the immune gene expression of the citrus. Additionally, we used AuNPs-PEI to deliver siRNA₄₃₁₀ to citrus cells, significantly reducing the expression of SDE4310 within 3 days. Although the suppression of SDE4310 expression did not inhibit the CLas proliferation, it increased the expression level of CsPR1, CsNPR1 and CsBIK1. This is also the first time that AuNPs-PEI has been found to be able to deliver exogenous plasmids into citrus cells and express the target protein, providing a new method for future studies on citrus HLB.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"38"},"PeriodicalIF":5.3,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An involvement of a new zinc finger protein PbrZFP719 into pear self-incompatibility reaction.","authors":"Ying Xu, Zhi-Heng Sui, Yi-Peng Ye, Lei Wu, Kai-Jie Qi, Min He, Lin Guo, Chao Gu, Shao-Ling Zhang","doi":"10.1007/s00299-024-03418-1","DOIUrl":"https://doi.org/10.1007/s00299-024-03418-1","url":null,"abstract":"<p><strong>Key message: </strong>This study indicated that the CCHC-type zinc finger protein PbrZFP719 involves into self-incompatibility by affecting the levels of reactive oxygen species and cellulose content at the tips of pollen tubes. S-RNase-based self-incompatibility (SI) facilitates cross-pollination and prevents self-pollination, which in turn increases the costs associated with artificial pollination in fruit crops. Self S-RNase exerts its inhibitory effects on pollen tube growth by altering cell structures and components, including reactive oxygen species (ROS) level and cellulose content. Presently, only a limited number of genes have been implicated in the gametophytic SI. In this study, the CCHC-type zinc finger proteins (ZFP), PbrZFP719, was found to be more highly expressed in pollen grains and pollen tubes than other ZFPs. Experimental over-expression of PbrZFP719 via pollen magnetofection and its knockdown using antisense oligonucleotides demonstrated that PbrZFP719 positively mediates pollen tube growth in pear. Further analyses revealed that variations in PbrZFP719 expression correlate with the changes in ROS levels and cellulose content at the tips of pollen tubes. Notably, PbrZFP719 expression was reduced in pollen tubes treated with self S-RNase. These results suggest that self S-RNase can inhibit pollen tube growth by decreasing ROS levels and cellulose content through the downregulation of PbrZFP719 expression. The information provide insights into a novel mechanism by which self S-RNase inhibits pollen tube growth during SI reaction and offers a refined approach for gene over-expression in pollen tube.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"37"},"PeriodicalIF":5.3,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining resistance indicators, metabolomes and transcriptomes to reveal correlations in disease and cold resistance in tea plant and analyze the key domain NB-ARC.","authors":"Min Li, Wenting Wang, Xiaodan Chen, Xiumei Lu, Yahui Huang","doi":"10.1007/s00299-024-03384-8","DOIUrl":"https://doi.org/10.1007/s00299-024-03384-8","url":null,"abstract":"<p><strong>Key message: </strong>Integration of resistance indicators, metabolomes, and transcriptomes to elucidate that there is a positive correlation between disease susceptibility and cold tolerance in tea plants. The flavonoid pathway was found to be the major metabolic and transcriptional enrichment pathway. A key domain NB-ARC was identified through joint analysis, along with analysis of key domains within the NB-ARC protein. Tea is a healthy beverage and the tea plant is a woody plant rich in secondary metabolites. In the face of abnormal climate change year by year, it is important to investigate the mechanisms by which tea plants resist both biotic and abiotic stresses. In this study, we found different tea plant cultivars were evaluated for cold and disease resistance have highly correlated. Subsequently, two cold and fungal resistant cultivars were screened from a Shuixian population that had been cold domesticated for 50 years, and transcriptome and metabolome assays were performed on the two materials under cold and anthracnose stresses, using Baiye Dancong as a control. The analyses found that differential metabolites were most enriched in the flavonoid pathway and differentially expressed genes were most enriched in the pathway related to disease course after pathogen stress and cold stress. Combined metabolome and transcriptome analyses identified 30 genes that were positively correlated with flavonoid content after pathogen stress and cold stress, of which the number of genes with NB-ARC structural domains was 11, which accounted for the largest proportion. These 11 genes with NB-ARC structural domains were analyzed by family analysis and found to be highly involved in different tissues transcriptomes of tea plants, indicating the importance of the NB-ARC structural domains in biotic and abiotic stresses, and providing a theoretical basis of analysis for the subsequent related studies. In this study, through the identification of resistance in different varieties of tea plant and the multi-omics approach, we found the genes related to the key structural domain NB-ARC, which lays the foundation for the study of biologically and abiologically important mechanisms in response to the disease in tea plant.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"34"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naa50 regulates ovule and embryo sac development in Arabidopsis.","authors":"Jinlin Feng, Jiachuan Tian, Weihong Cao","doi":"10.1007/s00299-025-03431-y","DOIUrl":"https://doi.org/10.1007/s00299-025-03431-y","url":null,"abstract":"<p><strong>Key message: </strong>N-terminal acetyltransferase Naa50 plays an important regulatory role in ovule development by indirectly promoting cell wall invertase 2/4 expression.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"35"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genomic analysis of SmbHLH genes and the role of SmbHLH93 in eggplant anthocyanin biosynthesis.","authors":"XinJin Yu, SiYu Cao, JinDi Wang, DaLu Li, YongJun He","doi":"10.1007/s00299-025-03429-6","DOIUrl":"https://doi.org/10.1007/s00299-025-03429-6","url":null,"abstract":"<p><strong>Key message: </strong>SmbHLH93can activate the expression of SmCHS, SmANS, SmDFR and SmF3H.Overexpression of SmbHLH93promotes anthocyanin biosynthesis. SmbHLH93can interact with SmMYB1 to promote anthocyanin accumulation. As an outstanding source of anthocyanins, eggplant (Solanum melongena L.) is extremely beneficial for human health. In the process of anthocyanin biosynthesis in eggplant, the basic helix-loop-helix (bHLH) transcription factor family plays a crucial role. However, the bHLH gene family is extensive, making it difficult to systematically screen and analyze their functions using conventional methods. We studied the phylogeny, gene structure, conserved motifs, promoter element, and chromosomal location of the 166 SmbHLH genes in the recently released eggplant genome. Through the analysis of transcriptomic data of eggplant peel treated with light, it was found that SmbHLH93 was the most responsive to light among those of unknown function. Additionally, it was discovered that SmbHLH93 plays a positive regulatory role in anthocyanin synthesis through dual-luciferase reporter assay(dual-LUC) and genetic transformation in Arabidopsis (Arabidopsis thaliana). Furthermore, experiments involving yeast two-hybrid (Y2H), luciferase complementation assay (Split-LUC), and tobacco transient transformation demonstrated that SmbHLH93 has the ability to interact with SmMYB1 in order to enhance anthocyanin accumulation. This study will serve as a foundation for exploring the role of SmbHLH transcription factors in anthocyanin biosynthesis in the future.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"36"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and functional characterization of CLG family genes reveal likely roles in epidermal development in Arabidopsis.","authors":"Qiuli Zhang, Tao Hong, Tian Su, Qiaolong Li, Aoni Xiang, Xinlong Chen, Guanghua He, Fangming Zhao, Renhong Wu","doi":"10.1007/s00299-024-03421-6","DOIUrl":"https://doi.org/10.1007/s00299-024-03421-6","url":null,"abstract":"<p><strong>Key message: </strong>We identified a CX₂CX₁₂CPXC motif and 11 CLG genes that regulate epidermal development by interacting with homeodomain leucine-zipper IV family proteins in Arabidopsis. Zinc finger proteins (ZFPs), the key regulators of plant growth and development, can be categorized based on the sequence patterns of zinc finger motifs. Here, by aligning the amino acid sequences of CFL1, AtCFL1, AtCFL2, GIRl, and GIR2, we identified the CX₂CX₁₂CPXC motif in their C-terminus, which differs from all the previously characterized canonical zinc finger motifs. A total of 11 non-canonical ZFPs were identified in Arabidopsis based on the pattern and we named these genes as the CLG family genes (CFL1-like genes). We further established that the family genes are randomly distributed on five chromosomes, containing two conserved motifs and possess various cis-acting elements associated with plant hormones, stress responses and tissue regulation. Moreover, the family genes exhibit a wide range of expression profiles, and all of these proteins have nuclear localization signals. They may affect epidermal development through interactions with homeodomain leucine-zipper IV (HD-ZIP IV) family proteins. Overall, these findings comprehensively characterize CLG family genes and lay a foundation for further elaborating their biological functions in plant growth and development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"32"},"PeriodicalIF":5.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.","authors":"Abhishek Bohra, Abha Tiwari, Shalini Pareek, Rohit Joshi, S J Satheesh Naik, Khushbu Kumari, Ram Lakhan Verma, Ashok K Parihar, Prakash G Patil, Girish P Dixit","doi":"10.1007/s00299-024-03414-5","DOIUrl":"https://doi.org/10.1007/s00299-024-03414-5","url":null,"abstract":"<p><p>Plant breeding needs to embrace genetic innovations to ensure stability in crop yields under fluctuating climatic conditions. Development of commercial hybrid varieties has proven to be a sustainable and economical alternative to deliver superior yield, quality and resistance with uniformity in a number of food crops. Cytoplasmic male sterility (CMS), a maternally inherited inability to produce functional pollen, facilitates a three-line system for efficient hybrid seed production strategies in crops. The CMS system has illustrated its potential as a robust pollination control mechanism to support the billion-dollar seed industry. In plants, CMS arises due to a genomic conflict between mitochondrial open reading frames (orfs) and nuclear-encoding restoration-of-fertility (Rf) genes, leading to floral abnormalities and pollen sterility. Research on pollen sterility and fertility restoration provides deeper insights into cytoplasmic-nuclear interplay in plants and elucidates key molecular targets for hybrid breeding in crops. More recently, programmable gene editing (e.g., TALEN, CRISPR-Cas) has emerged as a promising tool to functionally validate CMS and Rf genes and obviate the need for pollen donors or Rf-genes for hybrid breeding. Modern genomic prediction models have allowed establishment of high-performing heterotic groups and patterns for sustaining long-term gain in hybrid breeding. This article reviews latest discoveries elucidating the molecular mechanisms behind CMS and fertility restoration in plants. We then present our perspective on how evolving genetic technologies are contributing to advance fundamental knowledge of the CMS-Rf genetic system for producing crop hybrids with high heterosis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"33"},"PeriodicalIF":5.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}