Plant Cell Reports最新文献

{"title":"Genome-wide analysis of NAC gene family in Brassica campestris ssp. chinensis and characterization of BcNAC134.","authors":"Shuilin Yuan, YingXin Lin, Yuan Wang, Changwei Zhang, Dong Xiao, Xilin Hou, Ying Li","doi":"10.1007/s00299-025-03611-w","DOIUrl":"10.1007/s00299-025-03611-w","url":null,"abstract":"<p><strong>Key message: </strong>204 BcNAC genes were identified and 98 BcNACs were differentially expressed in leaves at four developmental stages. BcNAC134 directly activates BcSAG12 expression and silencing of BcNAC134 delayed leaf senescence. Leaf senescence is a key factor restricting the development of non-heading Chinese cabbage (NHCC, Brassica campestris (syn. Brassica rapa) ssp. chinensis), which resulted in decreased product quality and shortened harvest period. The NAC (NAM, ATAF1/2, and CUC2) family is plant-specific and involved in multiple developmental processes. In this study, a total of 204 BcNACs were identified based on the NHCC genome. These genes were unevenly distributed on 10 chromosomes and classified into 13 groups. A total of 10 conserved motifs were identified by gene structure and motif analysis, among which motifs 1-6 were highly conserved, while motifs 7-10 existed in only a few groups. RNA-seq and RT-qPCR results both indicated that 10 BcNACs were influenced by age-dependent leaf senescence. Further validation showed that silencing of BcNAC134 in NHCC resulted in delayed leaf senescence and was accompanied by down-regulation of senescence-associated genes (SAGs). BcNAC134 localized in the nucleus and had transcription activation activity. BcNAC134 could directly bind to and activate BcSAG12, indicating that BcNAC134 promotes leaf senescence by activating BcSAG12. These results provide a foundation for further understanding the role of BcNACs in leaf senescence.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"224"},"PeriodicalIF":4.5,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aluminum-mediated alleviation of manganese toxicity in Malus robusta Rehd.: insights into mechanisms and implications.","authors":"Xintong Su, Wanying Xie, Jie Shen, Yu Tian, Zhiyuan Li, Xuqiang Qiao","doi":"10.1007/s00299-025-03610-x","DOIUrl":"10.1007/s00299-025-03610-x","url":null,"abstract":"<p><strong>Key message: </strong>This study elucidates the Al-mediated Mn detoxification mechanism in M. robusta, incorporating organ-specific localization, subcellular distribution, transporter regulation, and ion competition. Manganese (Mn) and Aluminum (Al) toxicity are major stressors that limit crop productivity in acidic soils. While studies mainly focus on the individual effects of Al or Mn, the interaction between them, especially how Al modulates Mn uptake, distribution, and homeostasis, remains understudied. This research investigates the synergistic effects of Al and Mn on Malus robusta Rehd., analyzing Mn content, subcellular distribution, Mn transporter expression, reactive oxygen species (ROS) homeostasis, and nutrient variations. We find that Al reduces Mn uptake, alleviating Mn-induced growth inhibition, evidenced by increased plant height, root elongation, and chlorophyll retention. Al also promotes antioxidant enzyme activity, including superoxide dismutase, glutathione, non-protein thiols, and proline, which are suppressed under Mn stress. Organ-specific analysis shows reduced Mn accumulation in roots, stems, and leaves, with slight increases in Mn in the cytoplasm and organelles of leaves. Gene expression analysis reveals changes in key Mn transporters (MTP, VIT, ZIP, NRAMP, YSL, CAX) under Al treatment, suggesting a coordinated regulatory mechanism. Phosphorus (P) and molybdenum (Mo) contents increase under Mn stress and are further enhanced by Al, while calcium (Ca), magnesium (Mg), and potassium (K) levels decrease, but Al alleviates this effect. This study provides insights into plant stress physiology, supporting future resistance breeding in fruit trees.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"223"},"PeriodicalIF":4.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The negative regulated immune mechanism of Skp1-Cullin1-F-box (SCF) E3 ubiquitin ligase-mediated HOS15-dependent responses in banana.","authors":"Xinyi Chen, Xingchen Jia, Ruizhou Fu, Maoli Wang, Hang Rong, Jinyi Wei, Zishu Wang, Mingyuan Wang","doi":"10.1007/s00299-025-03614-7","DOIUrl":"10.1007/s00299-025-03614-7","url":null,"abstract":"<p><strong>Key message: </strong>Under Foc TR4 stress, MaSKP1-1, MaCULI1, and MaHOS15 form SCF^HOS15-type E3 ubiquitin ligase, which inhibits the expression level of SA signal-related factors to regulate the immune process of bananas. Banana wilt disease, caused by Fusarium oxysporum f. sp. cubense, tropical race 4 (Foc TR4), poses a significant threat to global banana production. In Musa spp., HOS15 is believed to play a role in enhancing the response to Foc TR4 stress by the salicylic acid signaling pathway. Previous studies have indicated that HOS15 functions as an F-box protein within the SCF ubiquitin ligase complex, contributing to plant biotic stress responses. However, the regulatory mechanisms of SCF in bananas remain poorly understood. In this study, we identified MaSkp1-1 and MaCUL1 as key components in bananas. Under Foc TR4 stress conditions, expression levels of MaSkp1-1 and MaCUL1 increased in the roots of the susceptible variety Williams, whereas their expression decreased in the resistant variety 'Nantianhuang'. The involvement of the MaSkp1-1-MaCUL1-HOS15 module in banana wilt disease was confirmed through yeast two-hybrid assays and bimolecular fluorescence complementation experiments. Arabidopsis thaliana lines overexpressing MaSkp1-1 (MaSkp1 OE) and MaCUL1 (MaCUL1 OE) exhibited reduced resistance to Foc TR4 infection. Furthermore, both MaSkp1-1 and MaCUL1 were found to mediate Arabidopsis's response to Foc TR4 stress by influencing the expression of salicylic acid-related genes. In conclusion, these findings provide new insights into the molecular mechanisms underlying the function of the MaSkp1-1-MaCUL1-HOS15 module in plant responses to Foc TR4 stress.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"222"},"PeriodicalIF":4.5,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsR126 IncRNA integrates AtWRKY46 and AtEM1 into ABA signaling in Arabidopsis.","authors":"Lingyun Chang, Shengyi Liu, Nan Zhang, Dongxu Yang, Ziguang Liu, Hongli Yuan, Rong Yan, Xingguo Lan, Yasushi Yukawa, Juan Wu","doi":"10.1007/s00299-025-03607-6","DOIUrl":"10.1007/s00299-025-03607-6","url":null,"abstract":"<p><strong>Key message: </strong>Pol III-dependent rice OsR126 lncRNA affects the salt tolerance of Arabidopsis by regulating OsR126/AtWRKY46/AtEM1 module, this discovery further elucidates the ncRNA-mediated molecular regulatory network of plant salt stress. Rice is an important food crop that is frequently affected by various abiotic stresses during growth. In particular, salt stress leads to ion toxicity and oxidation, severely restricting rice yield and quality. Abscisic acid (ABA) is an important anti-stress hormone in plants. Stress can lead to ABA accumulation and signaling that mediates plant stress adaptation in various ways. However, there are many unsolved mysteries regarding the mechanism of ABA action related to stress. Stress-related long non-coding RNAs (lncRNAs) transcribed by RNA polymerase II (Pol II) play crucial roles in stress response by influencing flanking gene expression, epigenetic regulation, endogenous antisense mechanisms, and other aspects in plants. This study discovered that OsR126 lncRNA responded to salt and ABA in rice, and the OsR126 lncRNA overexpression significantly reduced the tolerance of germinating seeds and root growth to saline stress in an ABA-dependent manner in Arabidopsis. In OsR126-AtOXs, AtEM1 expression significantly increased, while AtWRKY46 expression was inhibited under ABA. Seed germination and root growth of AtEM1-OXs and wrky46 showed strong ABA sensitivity. Meanwhile, AtWRKY46 directly bound to the W-box in the AtEM1 promoter and inhibited its expression. This suggests that OsR126 lncRNA affects the salt tolerance of Arabidopsis by integrating AtWRKY46 into ABA signaling to mediate AtEM1 transcription. Discovery of the OsR126/AtWRKY46/AtEM1 regulatory module helps to further elucidate the non-coding RNA (ncRNA)-mediated molecular regulatory network of plant salt stress, provides a new gene resource to breed stress-resistant varieties, and has important agricultural application value.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"221"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of PtrCWINV2 accelerates shoot growth and wood development in hybrid poplar (Populus davidiana × Populus bolleana).","authors":"Ruichao Liu, Hongying Wang, Junjie Zhang, Jiangting Yang, Pan Guo, Yueting Qi, Xiaotong Guo, Chaoxia Lu, Chunyan Yu, Hongxia Zhang","doi":"10.1007/s00299-025-03609-4","DOIUrl":"10.1007/s00299-025-03609-4","url":null,"abstract":"<p><strong>Key message: </strong>PtrCWINV2 accelerates shoot growth and wood development in poplar via increased cellulose/lignin content, promoted sucrose allocation, enhanced hexose accumulation, and improved photosynthetic capacity. The transport of carbohydrates from source to sink organs is one of the major determinants affecting plant growth and development. Cell wall invertase (CWINV) of the invertase family catalyzes the decomposition of sucrose into hexoses, and regulates the loading and unloading of sugar in phloem. However, its functions in trees remain poorly understood. In this study, the poplar PtrCWINV2 gene was overexpressed in the hybrid clone Shanxin Yang (Populus davidiana × Populus bolleana), and its biological function was characterized. Overexpression of PtrCWINV2 promoted shoot growth and wood formation in transgenic poplar, as evidenced by the increased plant height, leaf number and size, stem diameter, and stem and leaf fresh weight. An increased xylem cell number and size, as well as phloem thickness, was also observed with microscopy. Further physiological analyses indicated that PtrCWINV2 overexpression modulated sucrose allocation and enhanced hexose accumulation along with photosynthetic carbon assimilation. Cellulose and lignin content, cellulose/lignin-related gene expression, soluble sugar accumulation, CWINV activity and photosynthetic rate were all improved in transgenic plants. Transcriptomic analysis revealed that the enriched pathways among the differentially expressed genes were well aligned with the physiological changes, corroborating the observed phenotypic correlation in transgenic plants. This study concludes that PtrCWINV2 represents a candidate gene for promoting tree growth and provides valuable insights for its application in the genetic improvement of wooden plants.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"219"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpSnRK2.7 interacts with CpPP2Cs to enhance salt tolerance via modulating oxidative stress in Cucurbita pepo.","authors":"Ke Xu, Ping Wang","doi":"10.1007/s00299-025-03613-8","DOIUrl":"10.1007/s00299-025-03613-8","url":null,"abstract":"<p><strong>Key message: </strong>CpSnRK2.7 interacts with CpPP2Cs and enhances salt stress tolerance in Cucurbita pepo by reducing oxidative damage, promoting proline accumulation, and maintaining photosynthetic efficiency. Sucrose non-fermenting-1-related protein kinase 2 (SnRK2) serves as a pivotal regulator of the ABA signaling cascade and plays an essential role in plant adaptation to abiotic stress. This study elucidated the role of the CpSnRK2.7 gene in Cucurbita pepo under salt stress conditions. Overexpression of CpSnRK2.7 significantly enhanced salt tolerance by alleviating cellular oxidative damage, as demonstrated by reduced accumulation of MDA, O₂^-, and H₂O₂, while concurrently increasing antioxidant enzyme activities and proline accumulation. Importantly, CpSnRK2.7-overexpressing plants displayed faster stomatal closure and maintained higher photosynthetic efficiency in response to salt stress and ABA treatment. Given that CpSnRK2.7 expression is induced by ABA, it is postulated to function within the canonical CpPYL/PYRs-CpPP2Cs-CpSnRK2.7 signaling module. Protein-protein interaction analysis via the STRING database and yeast two-hybrid assays revealed direct interactions between CpSnRK2.7 and CpPP2C1 as well as CpPP2C3. Notably, the interaction between CpPP2C1 and multiple CpPYL/PYRs was significantly enhanced following ABA treatment. Taken together, these findings indicate that CpSnRK2.7 plays a pivotal role in salt stress adaptation through ABA-mediated signaling and identifies potential targets for enhancing crop tolerance to salinity.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"220"},"PeriodicalIF":4.5,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering UV-C-induced transcriptomic shifts in tomato: defence mechanisms, stress responses, and photoreceptor regulation.","authors":"Gwo Rong Wong, Kah Ooi Chua, Aida Nabila Rahim, Kausalyaa Kaliapan, Jennifer Ann Harikrishna, Narayanan Ramakrishnan, Purabi Mazumdar","doi":"10.1007/s00299-025-03604-9","DOIUrl":"10.1007/s00299-025-03604-9","url":null,"abstract":"<p><strong>Key message: </strong>This study reveals transcriptomic reprogramming in tomato under UV-C, highlighting enriched defence pathways, calcium signalling, enhanced pathogen tolerance, increased defence enzyme activity, and photoreceptor-mediated UV-C perception, linking light signalling to stress adaptation. UV-C radiation, when applied at hormetic dosage, acts as a mild abiotic stressor in plants, triggering beneficial responses that enhance yield and bioactive compound production. However, transcriptomic responses to UV-C remain underexplored. This study investigates UV-C-induced transcriptomic changes in two tomato cultivars with differing UV-C susceptibility; Red Rock (susceptible) and Super Star (comparatively tolerant). RNA-seq analysis revealed 3540 DEGs in Red Rock and 2678 in Super Star, with a higher proportion of up-regulated genes in both cultivars after UV-C exposure. GO analysis indicated a conserved response, with enriched pathways associated with defence and stress adaptation. KEGG and PGSEA analyses highlighted significant enrichment in phenylpropanoid biosynthesis, plant-pathogen interaction, and fatty acid degradation pathways. Calcium-binding and signalling genes were predominantly up-regulated, suggesting a key role in UV-C-induced defence responses. RT-qPCR analysis of 17 selected genes, in both cultivars, showed the highest change in expression for Solyc06g069740.1, encoding a calcium-binding EF-hand family protein involved in signal transduction and Solyc05g050350.2, a Cyclic nucleotide-gated ion channel 1 involved in ion transport across the membrane during defence response. Detached leaf assays with Botrytis cinerea and Sclerotinia sclerotiorum demonstrated enhanced pathogen tolerance in UV-C-treated plants, with reduced lesion sizes and increased defence enzyme activity, including chitinase, phenylalanine ammonia-lyase, β-1,3-glucanase, and polyphenol oxidase, compared to fungal-treated controls. Further analysis of photoreceptors revealed upregulation of UV Resistance Locus 8 and Cryptochromes, linking UV-C perception to downstream signalling. These findings provide insights into UV-C-induced defence mechanisms and potential applications for improving crop resilience and productivity.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"217"},"PeriodicalIF":4.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulled GA signal orchestration proteome reconfiguration and redundancy cycling via PtGA2ox10-mediated metabolic gatekeeping in Chinese pine seeds.","authors":"Xinxiu Zuo, Shuai Liu, Yanjun Li, Yanyan Zhang, Duxian Lu, Shihui Niu, Xiaojuan Li, Jinxing Lin, Yaning Cui","doi":"10.1007/s00299-025-03596-6","DOIUrl":"10.1007/s00299-025-03596-6","url":null,"abstract":"<p><strong>Key message: </strong>This study reveals the molecular mechanisms of seed dormancy and germination in Chinese pine, highlighting the key roles of GA and ABA pathways and specific genes in regulating germination, growth, and stress resistance. Chinese pine (Pinus tabuliformis) is an ecologically and economically vital conifer species in China, serving critical functions in forest regeneration, soil conservation, and carbon sequestration. Despite its ecological importance, the molecular mechanisms governing seed dormancy release and germination in this species remain poorly understood. Using an integrative approach that combines advanced microscopy, hormone profiling, metabolomics, and whole transcriptome sequencing, we uncovered novel molecular insights into these crucial developmental processes. Optical and transmission electron microscopy revealed that protein bodies aggregated in aleurone cells during dormancy release. High-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis revealed distinct hormonal antagonism: gibberellin (GA) levels peaked during dormancy release (S2), with GA3 increased by 8.1-fold (from 41.37 ± 8.68 ng/g to 336.97 ± 106.14 ng/g), whereas abscisic acid (ABA) decreased by 63.6% (from 54.39 ± 1.83 ng/g at S1 to 19.80 ± 0.73 ng/g at S3), which negatively correlated with seed germination. Moreover, these hormonal changes were accompanied by profound metabolic reprogramming, which included a 6.0-fold increase in fructose content and a 12.3-fold increase in glucose content, whereas sucrose levels decreased by 48.1%, indicating enhanced carbohydrate mobilization during the germination phase (S3). Importantly, our transcriptomic analyses identified three novel regulatory genes (PtGA2ox10, PtRGA1, and PtABI2) with distinct expression patterns and functional roles during dormancy-germination transitions. Most significantly, we provide the first experimental evidence that PtGA2ox10 plays a dual regulatory role in both germination control and stress response pathways, a previously unrecognized function in conifers. These findings fundamentally advance our understanding of pine seed biology and establish new research directions in conifer molecular physiology.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"218"},"PeriodicalIF":4.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145125866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Volatile organic compounds from Bacillus velezensis FZB42 remodel Arabidopsis root architecture by an auxin-dependent mechanism.","authors":"Ailing Ye, Liang Yue, Andéole Niyongabo Turatsinze, Xiaofan Xie, Zongyu Zhang, Gaofeng Chen, Lingling Wu, Qin Zhou, Yun Wang, Meilan Zhang, Yubao Zhang, Jiecai Zhao, Ruoyu Wang","doi":"10.1007/s00299-025-03601-y","DOIUrl":"10.1007/s00299-025-03601-y","url":null,"abstract":"<p><strong>Key message: </strong>Volatile organic compounds produced by plant growth-promoting rhizobacteria promote lateral root development by modulating the auxin signaling pathway in Arabidopsis thaliana, thereby remodeling root architecture. Volatile organic compounds (VOCs) emitted by plant growth-promoting rhizobacteria (PGPR) have been shown to promote both shoot and root growth in plants. While VOCs are known to modulate root architecture, the underlying mechanisms remain poorly understood. In this study, we demonstrate that VOCs released by Bacillus velezensis FZB42 significantly promote primary root elongation and increase lateral root (LR) development in Arabidopsis thaliana, thereby altering root architecture. This study indicates that VOC-mediated modulation of root architecture is closely associated with auxin signaling, particularly its polar transport. Notably, the promotive effects of VOCs on lateral root formation were nearly abolished in auxin signaling mutants, including pin2 and axr1-12. Our results further demonstrate that treatment with FZB42-VOCs does not rescue the lateral root deficiency phenotype in the auxin core components arf7 arf19 double mutants. VOCs were found to stimulate the emergence of lateral root primordia (LRP) and to induce the expression of the auxin-responsive marker DR5:GFP in pre-existing LRPs. Additionally, VOCs were found to modulate the expression of auxin efflux carriers, such as PIN1 and PIN2, and to induce DR5:GFP expression in both primary and lateral roots. Treatment with NPA, an auxin transport inhibitor, further confirmed that VOC-mediated remodeling of root architecture is dependent on auxin polar transport. In conclusion, these findings suggest that VOCs enhance auxin response during early lateral root development by modulating auxin distribution and downstream signaling, thereby stimulating lateral root formation. These findings provide valuable insights into microbe-mediated root development and could inform sustainable agricultural practices involving PGPR.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"215"},"PeriodicalIF":4.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unresolved ER stress restricts in vitro plant cell totipotency.","authors":"Patricia Corral Martinez, Charlotte Siemons, Michael Schon, Marije Vos, Anneke Horstman, Ruud de Maagd, Jose María Seguí-Simarro, Kim Boutilier","doi":"10.1007/s00299-025-03586-8","DOIUrl":"10.1007/s00299-025-03586-8","url":null,"abstract":"<p><strong>Key message: </strong>Many plant cells can be induced to regenerate in vitro. We show that successful regeneration during microspore-derived embryo culture relies in part on the ability of embryogenic cells to resolve tissue culture-induced ER stress. During Brassica napus microspore embryogenesis, the immature male gametophyte is induced by a heat stress treatment to develop into a haploid embryo. Different multicellular embryogenic structures develop in response to heat stress, each with a different potential to complete embryo development. The underlying factors that determine the ability of these initially embryogenic structures to successfully complete embryo development are not known. We show that all embryogenic structures exhibit elements of endoplasmic reticulum (ER) stress, like ER expansion and protein-filled ER cisternae, but that the ER stress response is amplified in embryogenic structures with a low potential to complete embryo development. ER stress was amplified even further by treating heat-stressed cultures with trichostatin A, a histone deacetylase inhibitor epidrug that promotes embryogenic cell formation. Pharmacological treatment of microspore-derived embryo cultures with small molecule modulators of ER stress provided further evidence for the role of ER stress in microspore embryo development. Our results suggest that (1) the inability of certain embryogenic structures to resolve their ER stress responses restricts their ability to complete embryo development, and (2) histone deacetylation enhances microspore embryogenesis in B. napus, in part through its activity as an abiotic stress inducer.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"214"},"PeriodicalIF":4.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}