Plant Cell Reports最新文献

{"title":"Production of human papillomavirus type 16 virus-like particles in Physcomitrella photobioreactors.","authors":"Paul Alexander Niederau, Maria Caroline Weilguny, Sarah Chamas, Caitlin Elizabeth Turney, Juliana Parsons, Marta Rodríguez-Franco, Sebastian N W Hoernstein, Eva L Decker, Henrik Toft Simonsen, Ralf Reski","doi":"10.1007/s00299-025-03602-x","DOIUrl":"10.1007/s00299-025-03602-x","url":null,"abstract":"<p><strong>Key message: </strong>First production of virus-like particles as a vaccine candidate in a non-vascular plant. Virus-like particles (VLPs) are self-assembling nanoparticles composed of viral structural proteins which mimic native virions but lack viral DNA and infectivity. VLPs are a resourceful class of biopharmaceuticals applied as subunit vaccines or as delivery vehicles for drugs and nucleic acids. Similar to viruses, VLPs are diverse in structure, composition, and assembly, requiring a tailored production platform aligned with the intended application. The moss plant Physcomitrella (Physcomitrium patens) is an emerging expression system offering humanized N-glycosylation, scalability, and adaptability to existing industry settings. Here, we used Physcomitrella to produce human papillomavirus (HPV) 16 VLPs. HPV VLPs are composed of the major structural protein L1 and are used as vaccines against HPV infections which are the main causal agent of cervical and other anogenital cancers. We characterized Physcomitrella chloroplast transit peptides, which we used for targeting of moss-produced L1 to chloroplasts, leading to higher recombinant protein yield compared to nuclear or cytoplasmic localization. We confirmed subcellular localization with confocal laser scanning microscopy and found L1 to accumulate within the chloroplast stroma. Production in 5-L photobioreactors yielded over 0.3 mg L1 per gram fresh weight. We established a purification protocol for moss-produced L1 using a combination of ammonium sulphate precipitation and cation exchange chromatography. Purified samples were subjected to a controlled dis- and reassembly, yielding fully assembled HPV-16 L1 VLPs. This is the first report of production, purification, and assembly of VLPs in a non-vascular plant.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"216"},"PeriodicalIF":4.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional roles of miR862b in regulating soybean resistance to Heterodera glycines.","authors":"Hui Wang, Wanqing Bai, Xiaoquan Zhan, Shumei Liu, Fengjiao Fan, Jie Luo, Zhuolu Wang, Qiumin Chen, Yuxi Duan, Chen Liu","doi":"10.1007/s00299-025-03603-w","DOIUrl":"10.1007/s00299-025-03603-w","url":null,"abstract":"<p><strong>Key message: </strong>The legume-specific miR862b, acting via targets such as GmGRX1, is associated with soybean responses to Heterodera glycines. Exogenous ds-miR862b reduces SCN root infection while moderately and transiently suppressing seed germination and early seedling growth. Soybean cyst nematode (SCN) disease has emerged as a significant threat to soybean production globally, causing substantial yield losses. Identifying key resistance molecular determinants is essential for enhancing soybean resistance to SCN. Previous studies have shown that miR862b exhibits a strong response in the early stages of SCN infection across different soybean varieties; however, its role in the mechanism and function of SCN tolerance remains unclear. This study utilized bioinformatics prediction and qRT-PCR validation to elucidate the initial response of miR862b to SCN and screen its target genes, including GmGRX1 (Glyma.14G057300). Functional validation was conducted using K599-induced transgenic soybean roots, further indicating that overexpressing pre-miR862b positively regulates soybean resistance to SCN while significantly suppressing the expression of its target gene GmGRX1. Conversely, transgenic lines with tandem repeats silencing miR862b exhibited reduced SCN resistance, accompanied by upregulated expression levels of the target gene GmGRX1. In addition, treatment with artificially synthesized ds-miR862b in soybeans inhibited SCN infection in roots while also moderately suppressing seed germination and seedling development. In summary, these findings provide a theoretical basis for elucidating the function of miR862b and its target gene GmGRX1 in regulating soybean resistance to SCN.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"213"},"PeriodicalIF":4.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid assessment of CRISPR gRNAs with optimized protoplast transformation in Maize.","authors":"Lauren Higa, Max Blank, Ella Hampson, Jenna Matsuyama, Keilah Wilkes, Ashlyn Uehara, Taren Bouwman, Keunsub Lee, Kan Wang, Michael Muszynski, Zhi-Yan Du","doi":"10.1007/s00299-025-03599-3","DOIUrl":"10.1007/s00299-025-03599-3","url":null,"abstract":"<p><p>We developed an optimized CRISPR/Cas9 gene editing system using maize mesophyll protoplasts to enable rapid evaluation of guide RNA (gRNA) activity. Using the tropical inbred line Tzi8, we improved protoplast isolation and transfection protocols, achieving high yields of 17.88 × 10⁶ viable protoplasts per gram fresh weight while extending post-transfection viability. Etiolated seedlings and vertical leaf cutting significantly enhanced protoplast recovery and viability. A transfection efficiency of ~ 50% was achieved using 10 µg of plasmid DNA; higher DNA inputs did not result in significant gains, resulting in a more resource-efficient approach. Protoplast viability was maintained for up to seven days post-transfection, allowing for downstream applications that require extended incubation. This optimized system was used to assess the editing efficiency of nine gRNAs targeting three key floral repressors (ZmCCT9, ZmCCT10, and ZmRap2.7) across four maize genotypes (Tzi8, CML277, B73, and B104). These floral repressor genes are involved in the photoperiod sensitivity of tropical maize, a major challenge in the effort to introduce tropical maize germplasm into temperate breeding programs. Editing efficiencies ranged from 0.4% to 23.7%, with some variation observed between gRNAs and genotypes. Although protoplast-based assays do not currently enable plant regeneration, this platform offers a rapid method for in vivo gRNA validation, reducing assay time from months to days. This work expands the gene editing toolkit for tropical maize, supporting efforts to overcome breeding barriers through gene editing.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"212"},"PeriodicalIF":4.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-integrated metabolomics maps functional divergence of microbial consortia in field-grown maize.","authors":"Musiwalo Samuel Mulaudzi, Lerato Pertunia Nephali, Fidele Tugizimana","doi":"10.1007/s00299-025-03600-z","DOIUrl":"10.1007/s00299-025-03600-z","url":null,"abstract":"<p><strong>Key message: </strong>The study provides actionable insights into modes of action of the three microbial biostimulants on maize plants under field conditions. The postulated framework indicates a divergence model involving (i) photoprotection, structural reinforcement, and defense priming, (ii) systemic metabolic reprograming for growth and defense, and (iii) hormonal signalling modulation for stress response. These insights offer a data-driven blueprint for the next generation of sustainable, effective, and field-ready bioformulations. Microbial consortia are currently recognized as a promising strategy for sustainable agriculture due to their ability to enhance plant growth, improve soil health, and mitigate environmental stresses. However, the biochemical and molecular mechanisms governing these beneficial effects on crops under field conditions remain poorly understood, and differential effects due to different microbial formulations are enigmatic. This study, therefore, aims to unravel the metabolic alterations, in maize plants, induced by three microbial biostimulants, under field conditions at different growth stages. Leaves from biostimulant-treated and untreated control maize plants were harvested at different time points. Metabolites were extracted using methanol. The extracts were analyzed on LC-MS/MS system. Computational metabolomics workflows and AI-driven strategies such as molecular networking and machine learning methods (PCA and OPLS-DA) were applied to mine and interpret spectral data. Machine learning models revealed the common and unique significant metabolites among the consortia at the vegetative stage. One of the key findings was that hydroxycinnamic acid (HCA) derivatives are the discriminatory metabolites differentiating the effects of the three microbial consortia on maize plants. Moreover, the results showed that consortia application significantly impacted primary and secondary maize metabolism, reprogramming biological pathways such as phenylalanine, tyrosine, and tryptophan biosynthesis, tyrosine metabolism, the citrate cycle (TCA cycle), flavone and flavonol biosynthesis, and flavonoid biosynthesis. These pathways are associated with plant defense, priming and development. Thus, this study sheds light on the complex molecular interactions between maize and microbial biostimulants under real-world conditions. It reveals that distinct microbial formulations differentially influence plant metabolism by reprogramming defense- and growth-related pathways. These actionable insights establish a foundational framework for functionally characterizing microbial consortia and pave the way for the rational design of next generation biostimulants tailored to specific crop needs and growth stages.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"211"},"PeriodicalIF":4.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma membrane maize Gγ protein MGG4 positively regulates seed size mainly through influencing kernel width.","authors":"Huiyu Zhao, Yudong Chen, Wei Li, Yuhang Ding, Yijun Wang","doi":"10.1007/s00299-025-03594-8","DOIUrl":"10.1007/s00299-025-03594-8","url":null,"abstract":"<p><strong>Key message: </strong>Plasma membrane Gγ protein MGG4, the candidate for maize yield QTL, positively regulates seed size mainly through affecting kernel width.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"210"},"PeriodicalIF":4.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome duplication drives transcriptome reprogramming in response to drought in alfalfa.","authors":"D F Santoro, A W Anderson, S N Alavi, V A Malatesta Pierleoni, D Rosellini","doi":"10.1007/s00299-025-03593-9","DOIUrl":"10.1007/s00299-025-03593-9","url":null,"abstract":"<p><strong>Key message: </strong>Genome doubling did not enhance drought tolerance in alfalfa, but may set the stage for long-term adaptation to drought through a novel transcriptional landscape. Whole genome duplication (WGD) has been shown to enhance stress tolerance in plants. Cultivated alfalfa is autotetraploid, but diploid wild relatives are important sources of genetic variation for breeding. Investigating how WGD affects gene expression in stress conditions could provide better understanding for use of diploid genetic resources. In this work, we compared the drought response of neotetraploid plants obtained by bilateral sexual polyploidization with diploid full sibs, by measuring physiological and biochemical traits and RNA-seq. Without drought, 4x plants had lower photosynthetic potential than 2x plants per unit leaf area, but larger leaves allowed them to outperform the per leaf photosynthetic potential of 2x plants. Physiological and biochemical traits were significantly affected by drought in both 2x and 4x plants, but the differences between ploidies were small and nonsignificant. Proline levels were higher in 4x than 2x plants, both in control and drought conditions, indicating that larger cells with higher volume-to-surface ratio of 4x  plants require a higher osmolyte concentration. RNA-seq and gene network analyses showed that more genes were affected by drought at 4x than at 2x level, with downregulation of hundreds of genes involved in photosynthesis and stomatal movement at 4x level, suggesting that WGD made the 4x plants more responsive to drought. Genes involved in proline, phytormone and cell wall functions were also transcriptionally affected by drought in 4x plants. We conclude that WGD did not immediately enhance drought tolerance in alfalfa, but may set the stage for long-term adaptation to drought through a novel transcriptional landscape.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"209"},"PeriodicalIF":4.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics analysis of gibberellin involved in far-red light-regulated internode elongation in cucumber seedlings.","authors":"Shuhao Li, Yang Xu, Jun Tian, Hanbing Zheng, Ji Sun, Haitao Wu, Fenglin Zhong","doi":"10.1007/s00299-025-03598-4","DOIUrl":"10.1007/s00299-025-03598-4","url":null,"abstract":"<p><strong>Key message: </strong>GA participates in FR light-induced internode elongation of cucumber by regulating the expression of genes/proteins related to aquaporins, expansins, cell wall biosynthesis, hormone metabolism, and signal transduction. This study investigated the effects of the interaction between far-red (FR) light and gibberellin (GA) on the internode elongation of cucumber (Cucumis sativus L. 'Zhongnong No. 26') seedlings through combined physiological, biochemical, transcriptomic, and proteomic analyses. The results revealed that FR light and GA significantly promoted internode elongation in cucumber seedlings, whereas a GA biosynthesis inhibitor (PAC) inhibited the promoting effect of FR light. Hormone content determination revealed that FR light and GA decreased the contents of abscisic acid (ABA), indole-3-acetic acid (IAA), cytokinin (CTK), and jasmonate (JA) in cucumber seedling internodes. Bioinformatics analysis revealed that the expression patterns of the Co-DEGs and Co-DEPs were consistent in the FR (WL combined with FR light) and WLG (WL, in which plants were sprayed GA) groups, as well as in the FRP (FR, in which plants were sprayed PAC) and WL (full-spectrum LED white light) groups, suggesting that the mechanisms of FR and GA were similar in these Co-DEGs and Co-DEPs. Further analysis of these Co-DEGs and Co-DEPs revealed that they were involved mainly in cell wall biosynthesis and modification, lignin synthesis, hormone metabolism, and signal transduction pathways. In conclusion, this study revealed the important role of GA in FR light-induced internode elongation in cucumber seedlings, and this promoting effect was achieved mainly through the regulation of aquaporins, expansins, hormone metabolism, and signal transduction-related genes/proteins. This study provides new insights into the molecular mechanism of FR light-induced internode elongation in cucumber seedlings.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 10","pages":"208"},"PeriodicalIF":4.5,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZmDof08, a zinc finger transcription factor, plays critical roles in photosynthesis in maize.","authors":"Ting Li, Jinjie Guo, Jiahao Zhang, Ping Deng, Yuanyuan Xu, Xinjia Li, Yu He, Lian Zhou, Chaofeng Li, Chaoxian Liu","doi":"10.1007/s00299-025-03591-x","DOIUrl":"10.1007/s00299-025-03591-x","url":null,"abstract":"<p><strong>Key message: </strong>The gene ZmDof08, which underlies the yellow-green leaf mutant phenotype in maize, enhances the activity of key enzymes involved in C₄ photosynthesis, leading to a significant improvement in photosynthetic efficiency. Improving the photosynthetic efficiency of maize to increase its yield has long been a key focus in global agricultural research. Maize possesses a rich resource of leaf color mutants, which serve as valuable materials for studying leaf photosynthesis. The ygl4 mutant, characterized by yellow-green leaves and controlled by a recessive single gene, exhibits significant reductions in pigment content, net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr), along with abnormal chloroplast development. Through map-based cloning and gene editing techniques, we confirmed that the impaired function of the ZmDof08 gene is the fundamental cause of the ygl4 phenotype. ZmDof08 encodes a transcription factor located in the nucleus and is highly expressed in maize leaves, with its expression being light-induced. In the overexpression transgenic lines OE-15 and OE-23, the fresh weight of the aboveground parts of the seedlings significantly increased, the pigment content in the leaves was notably elevated, and the activities of key photosynthesis-related enzymes phosphoenolpyruvate carboxylase (PEPC), pyruvate orthophosphate dikinase (PPDK), NADP-dependent malic enzyme (NADP-ME), and NADP-dependent malate dehydrogenase (NADP-MDH) were also significantly enhanced. RNA-seq analysis revealed that ZmDof08 is implicated in the formation of the light-harvesting complex. This study provides a feasible path for the molecular design breeding of high photosynthetic efficiency maize varieties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 9","pages":"207"},"PeriodicalIF":4.5,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key candidate genes regulating hundred-grain weight in the maize inbred line Ye107 from Southern China.","authors":"Xiaoli Hong, Ranjan K Shaw, Fuyan Jiang, Jiachen Sun, Yaqi Bi, Xiao Wu, Tao Dai, Fengli Ye, Jiaguo Zhu, Tingting Guan, Chunxia Xu, Xingming Fan","doi":"10.1007/s00299-025-03595-7","DOIUrl":"10.1007/s00299-025-03595-7","url":null,"abstract":"<p><strong>Key message: </strong>A potential candidate gene regulating maize hundred-grain weight was identified in the Ye107 inbred line through a genome-wide association study and QTL mapping, providing insights for high-yield maize breeding. Hundred-grain weight (HGW) is a key agronomic trait that significantly affects maize yield. Identifying HGW-related candidate genes is crucial for genetic improvement of maize. To overcome the limitations of traditional association panels in dissecting complex traits and to improve detection power and accuracy, this study constructed a multi-parent population (MPP). The MPP, consisting of 482 F₇ recombinant inbred lines (RILs), was developed by crossing three elite maize inbred lines (D39, R-2-1-1 and YML1218) with a backbone inbred line, Ye107. The study integrated two complementary approaches: genome-wide association study (GWAS) for fine-mapping and quantitative trait loci (QTL) mapping, which is effective at detecting major-effect loci. Using these methods, 42 SNPs and 17 QTLs significantly associated with HGW were identified. Through phenotypic variation analysis, functional annotation, and qRT-PCR validation, five candidate genes, including Zm00001eb225640, Zm00001eb225650, Zm00002eb228310, Zm00004eb228320, and Zm00001eb378820 were identified. Among them, Zm00001eb378820, which encodes the transcription factor GRF13 (GRFTF13), was identified as a key candidate gene regulating HGW in maize. Genomic estimated breeding values (GEBVs) indicated that population pop3 (YML1218 × Ye107) exhibited higher values than other subpopulations, suggesting its potential for high-yield breeding. These findings offer critical genetic targets for increasing maize yield and highlight the advantage of integrating MPPs with multiple genetic analysis strategies to dissect complex quantitative traits.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 9","pages":"206"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12402048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144966114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analyses highlight wheat TaSPL4-D as a promising candidate gene to optimize plant architecture traits in rice.","authors":"Li Li, Fu Shi, Lizhi Yin, Junli Chang, Mingjie Chen, Yuesheng Wang, Guangxiao Yang, Guangyuan He, Yin Li","doi":"10.1007/s00299-025-03597-5","DOIUrl":"10.1007/s00299-025-03597-5","url":null,"abstract":"<p><strong>Key message: </strong>Comparative expression analyses between wheat and rice highlight that TaSPL4 is involved in plant architecture traits, and heterologous expression of TaSPL4-D in rice reduces plant height and increases tiller number.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 9","pages":"205"},"PeriodicalIF":4.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}