Plant Cell Reports最新文献

{"title":"DcNCED2 promotes ABA synthesis via carotenoid degradation and enhances drought resistance in carrot.","authors":"Rong-Rong Zhang, Ya-Hui Wang, Xue-Feng Peng, Yu-Jie Sun, Nan Zhang, Ai-Sheng Xiong","doi":"10.1007/s00299-025-03467-0","DOIUrl":"https://doi.org/10.1007/s00299-025-03467-0","url":null,"abstract":"<p><strong>Key message: </strong>Carrot DcNCED2 gene can improve the activity of antioxidant substances, enhance the drought tolerance of plant, and play regulatory roles in the degradation of carotenoids and the synthesis of ABA. Carrot (Daucus carota L.) is a biennial root vegetable crop of Apiaceae. In the process of growth and development, carrot is always subjected to drought stress, resulting in the decline of yield and quality. 9-cis-epoxycarotenoid dioxygenase (NCED) is an important rate-limiting enzyme in the pathway of carotenoid degradation and ABA synthesis, which can directly affect the drought resistance of plants. It is scientifically important to study the molecular mechanism of carrot DcNCED gene in response to drought stress. In this study, expression specificity analysis of DcNCED2 gene showed that the expression level of DcNCED2 gene reached the highest value at 60-75 d after sowing. DcNCED2 gene was transferred into Arabidopsis thaliana and carrot by constructing plant overexpression vector. The transgenic A. thaliana was found to exhibit a drought-tolerant phenotype with longer root length, higher SOD and POD activities, lower MDA content, higher ABA content and related gene expression, and lower lutein and β-carotene content. The results indicated that DcNCED2 gene could improve the drought tolerance of the seedling. The ABA content in leaf of overexpressed DcNCED2 carrot was significantly increased, while the contents of lutein, α-carotene and β-carotene were decreased compared with the wild carrot. The changes of expression levels of most related genes were consistent with the above results. These results indicated that DcNCED2 gene could promote the degradation of carotenoids and the synthesis of ABA in carrot leaves and thus achieve the regulation of abiotic stress in carrot plants.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"75"},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic proteome and acetylome profiling reveals key regulators of sucrose accumulation in sugarcane.","authors":"Miao Wang, Ao-Mei Li, Zhong-Liang Chen, Cui-Xian Qin, Fen Liao, You-Qiang Pan, Prakash Lakshmanan, Xiao-Feng Li, Dong-Liang Huang","doi":"10.1007/s00299-025-03449-2","DOIUrl":"https://doi.org/10.1007/s00299-025-03449-2","url":null,"abstract":"<p><strong>Key message: </strong>Lysine acetylation and protein abundance both play crucial roles in regulating sucrose accumulation in sugarcane, with 73 dual-function proteins identified as potential targets for molecular breeding to enhance sucrose levels. Lysine acetylation plays a crucial role in regulating various biological processes in plants, but its role in sucrose accumulation in sugarcane remains unexplored In this study, we conducted a comprehensive quantitative proteome and acetylated proteome analysis on the leaves of two sugarcane genotypes with high and low sucrose levels at early, middle, and late stages of sucrose accumulation. Quantitative proteome analysis identified 2363 differentially abundant proteins (DAPs), of which 165 were associated with sugar metabolism pathways, providing more targets for improving sucrose content in sugarcane. The acetylated proteome analysis identified 1397 differentially acetylated proteins (DAcPs) with 2377 acetylation sites. Many DAcPs were also involved in sugar metabolism, demonstrating that lysine acetylation is associated with sucrose accumulation. A comparison of the DAPs and DAcPs identified 650 overlapping proteins, with 73 of them related to sugar metabolism, confirming dual regulatory roles of protein abundance and acetylation in sucrose accumulation in sugarcane. These 73 proteins serve as targets for sucrose improvement with dual regulatory effects. Our data also suggest that histone acetylation and nitrogen metabolism may be related to sucrose accumulation. This work enhances our understanding of the mechanisms regulating sucrose accumulation and proposes targets for improving sucrose content in sugarcane through molecular breeding.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"74"},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of the SmPHR gene family in Salvia miltiorrhiza and SmPHR7-mediated response to phosphate starvation in Arabidopsis thaliana.","authors":"Xinxin Wang, Renjun Qu, Shiwei Wang, Jiaming Peng, Juan Guo, Guanghong Cui, Tong Chen, Meilan Chen, Ye Shen","doi":"10.1007/s00299-025-03461-6","DOIUrl":"https://doi.org/10.1007/s00299-025-03461-6","url":null,"abstract":"<p><strong>Key message: </strong>This study reveals the transcripts of S. miltiorrhiza in response to phosphate deficiency, identifies 18 SmPHRs in the genome, and tentatively establishes a role for SmPHR7 in regulating phosphate starvation. Phosphorus is essential for plant growth and development, and phosphate deficiency is a common nutritional stress. Salvia miltiorrhiza (Danshen) is a traditional Chinese herb whose main active medicinal secondary metabolite is used in the treatment of heart disease. However, the physiological and molecular effects of phosphate starvation in S. miltiorrhiza have not been well studied. Here, we first investigated the effect of phosphate starvation on the growth and major medicinal compounds. Biomass decreased with lower phosphate concentrations, while the accumulation of compounds varied in S. miltiorrhiza. Transcriptome analysis showed that phosphate starvation affected the expression of genes involved in processes such as glycolysis/gluconeogenesis, glycerolipid metabolism, and phenylpropanoid biosynthesis. Phosphate starvation response (PHR) transcription factors play an important role in the phosphate starvation response, and we identified 18 PHR family genes in S. miltiorrhiza, distributed across 8 chromosomes. The expression levels of different SmPHR family members in roots and shoots differ in response to phosphate starvation. SmPHR7, which is highly expressed in response to phosphate starvations, was selected for further functional characterization. SmPHR7 has transcriptional activation activity and is localized in the nucleus. Furthermore, the expression of SmPHR7 in the Arabidopsis thaliana mutant phr (SmPHR7-OX) is shown to partially rescue the phosphate starvation phenotype. The expression of the Pi starvation-induced (PSI) gene in SmPHR7-OX showed a significant induction compared to the phr mutant under phosphate starvation. The identification of the SmPHR gene family significantly contributes to a broader understanding of phosphate starvation signaling in S. miltiorrhiza.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"73"},"PeriodicalIF":5.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel toolbox of GATEWAY-compatible vectors for rapid functional gene analysis in soybean composite plants.","authors":"Joffrey Mejias, Alexandra Margets, Melissa Bredow, Jessica Foster, Ekkachai Khwanbua, Jackson Goshon, Thomas R Maier, Steven A Whitham, Roger W Innes, Thomas J Baum","doi":"10.1007/s00299-025-03458-1","DOIUrl":"https://doi.org/10.1007/s00299-025-03458-1","url":null,"abstract":"<p><strong>Key message: </strong>We developed a set of GATEWAY vectors to accelerate gene function analysis in soybean composite plants to rapidly screen transgenic roots and investigate subcellular localization, protein-protein interactions, and root-pathogen interactions. The generation of transgenic plants is essential for plant biology research to investigate plant physiology, pathogen interactions, and gene function. However, producing stable transgenic plants for plants such as soybean is a laborious and time-consuming process, which can impede research progress. Composite plants consisting of wild-type shoots and transgenic roots are an alternative method for generating transgenic plant tissues that can facilitate functional analysis of genes-of-interest involved in root development or root-microbe interactions. In this report, we introduce a novel set of GATEWAY-compatible vectors that enable a wide range of molecular biology uses in roots of soybean composite plants. These vectors incorporate in-frame epitope fusions of green fluorescent protein, 3x-HA, or miniTurbo-ID, which can be easily fused to a gene-of-interest using the GATEWAY cloning system. Moreover, these vectors allow for the identification of transgenic roots using either mCherry fluorescence or the RUBY marker. We demonstrate the functionality of these vectors by expressing subcellular markers in soybean, providing evidence of their effectiveness in generating protein fusions in composite soybean plants. Furthermore, we show how these vectors can be used for gene function analysis by expressing the bacterial effector, AvrPphB in composite roots, enabling the identification of soybean targets via immunoprecipitation followed by mass spectrometry. Additionally, we demonstrate the successful expression of stable miniTurbo-ID fusion proteins in composite roots. Overall, this new set of vectors is a powerful tool that can be used to assess subcellular localization and perform gene function analyses in soybean roots without the need to generate stable transgenic plants.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"72"},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification unravels the role of the arabinogalactan peptide (AGP) gene family in cotton plant architecture.","authors":"Jungfeng Tang, Teame Gereziher Mehari, Dongmei Qian, Ruochen Li, Zhengyang Chen, Zitong Zhou, Yuchun Yan, Haodong Chen, Wei Wang, Baohua Wang","doi":"10.1007/s00299-025-03460-7","DOIUrl":"10.1007/s00299-025-03460-7","url":null,"abstract":"<p><strong>Key message: </strong>In our study, we identified the gene Gohir.A08G240900 as a potential target for regulating cotton plant height, providing a genetic basis for enhancing cotton morphology. Arabinogalactan peptides are a class of hydroxyproline-rich proteins widely distributed in plants that participate in many life processes, including growth and development, cell division and even plant reproductive development. In this study, we identified 122 members of the AGP gene family via genome-wide identification in six cotton species. Through phylogenetic tree analysis, the AGP family was divided into six different subgroups. A core yet variable region composed of proline, hydroxyproline, serine, threonine, and alanine (PAST) was identified among these members. Furthermore, Ka/Ks analysis revealed that the AGP gene family underwent multiple fragment duplication events. Additionally, we analyzed the 1.5 kb upstream cis-acting elements of all upland cotton family members and identified numerous functional elements associated with growth and development, suggesting a close relationship among the family members. The results of RT‒qPCR analysis revealed that the expression level of Gohir.A08G240900 was significantly different among the four upland cotton varieties, with significant differences in plant height. Virus-induced gene silencing (VIGS) experiments revealed that the height of Gohir.A08G240900 gene-silenced plants significantly decreased. The results revealed that Gohir.A08G240900 may affect plant growth and development and may be a potential functional gene regulating cotton plant height.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"71"},"PeriodicalIF":5.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of SlIQMs and the regulatory effect of calcium on tomato seedlings under drought stress and phytohormone treatment.","authors":"Jing Cui, Junrong Xu, Jin Qi, Xuefang Lu, Yunzhi Liu, Jingli Xiong, Wenjin Yu, Changxia Li","doi":"10.1007/s00299-025-03459-0","DOIUrl":"https://doi.org/10.1007/s00299-025-03459-0","url":null,"abstract":"<p><strong>Key message: </strong>SlIQMs were identified, exogenous calcium and phytohormones induced their expression. SlIQMs's function were verified by VIGS. Calcium synergistically promoted seedling growth with ABA, IAA, MeJA and antagonized growth inhibition with GA₃ or SA. The IQM genes, are crucial members of the calmodulin-binding protein family, play pivotal roles in plant growth and stress response. However, the existence and impact of IQM in tomato remain unclear. This study demonstrates that the SlIQMs are randomly distributed across the 4 chromosomes of tomato and exclusively located within the nucleus. Phylogenetic analysis classifies the SlIQMs into 3 distinct subclasses. Analysis of cis-acting elements reveals that SlIQMs may function in stress or hormone process. Quantitative reverse-transcriptase PCR analysis further testified that polyethylene glycol (PEG), abscisic acid (ABA), indole acetic acid (IAA), gibberellin (GA₃), methyl jasmonate (MeJA), and salicylic acid (SA) induce expression levels of SlIQM1/2/3/5/6/7. Furthermore, exogenous calcium significantly alleviates detrimental effects on seedlings growth leaded by drought stress. Moreover, the relationships between hormones and calcium were explored. The results showed that calcium synergistically promoted the seedlings growth with ABA, IAA and MeJA, however antagonistic effects on inhibiting growth are observed between calcium and GA₃ or SA. The virus-induced silencing of 6 candidate genes caused growth inhibition of tomato seedlings under drought stress and phytohormone treatment. These findings lay the foundation for a comprehensive study of the structure and biological function of SlIQM genes and the interaction between calcium and different plant hormones on plant growth.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 4","pages":"70"},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interacting MeZFP29 and MebZIPW regulates MeNRT2.2 from cassava responding to nitrate signaling.","authors":"Wenbin Li, Xiaoling Yu, Pingjuan Zhao, Shuxia Li, Liangping Zou, Xiuchun Zhang, Jiuhui Li, Mengbin Ruan","doi":"10.1007/s00299-025-03455-4","DOIUrl":"10.1007/s00299-025-03455-4","url":null,"abstract":"<p><strong>Key message: </strong>Cassava is a significant tropical cash crop. MeZFP29 interacting with MebZIPW improves MeNRT2.2, encoding a high-affinity nitrate transporter, through binding to NREs under low nitrate and shows a nitrate-signaling-triggered regulation. Cassava (Manihot esculenta) is a globally significant tropical root crop and exhibits exceptional adaptability to native soil fertility. MeNRT2.2 encodes a high-affinity nitrate transporter in cassava and heterologous overexpression of MeNRT2.2 in Arabidopsis increases nitrate transportation and utilization under nitrogen scarcity. However, the responsive mechanism of MeNRT2.2 to nitrate remains unclear. In this study, we identified a nitrate-responsive fragment of 450 bp located upstream of the start codon of MeNRT2.2, and two potential regulators, MeZFP29 and MebZIPW, of MeNRT2.2. Two regulators specifically bound to nitrate-responsive cis-element (NRE), i.e., TGCATT and CAGATG, in the 450 bp fragment and together significantly stimulated promoter activity. Furthermore, we confirmed the interaction between two regulators in vivo and in vitro via Y2H, BiFC, Co-IP, and GST-pull-down assays. In addition, the distribution of MeZFP29 and its heterodimers (MeZFP&MebZIPW) are determined by nitrate signaling, i.e., in the cytoplasm and nucleus under nitrogen limitation, and predominantly in nucleus under sufficient nitrate. In contrast, MebZIPW consistently localizes to the cytoplasm and nucleus regardless of nitrate conditions. Moreover, overexpression of MeZFP29 in Arabidopsis enhanced growth and chlorophyll content, particularly, under low nitrate conditions, while MebZIPW did not. These findings not only confirm the regulation of MeZFP29 and MebZIPW on MeNRT2.2, but also illustrate the nitrate signaling-triggered promotion and feedback on MeNRT2.2. Our study provides a novel approach to enhancing nitrogen-use efficiency in cassava by modulating the regulators under moderate nitrogen levels as low as possible.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"69"},"PeriodicalIF":5.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of CBL and CIPK gene families in bermudagrass reveals the CdCIPK29-A1 as a stem growth angle regulator.","authors":"Bing Zhang, Jianxiu Liu","doi":"10.1007/s00299-025-03457-2","DOIUrl":"10.1007/s00299-025-03457-2","url":null,"abstract":"<p><strong>Key message: </strong>Genome-wide analysis of CBL and CIPK gene family was conducted in bermudagrass while a functional role in stem growth angle regulation was established for CdCIPK29-A1 via the generation of molecularly modified Arabidopsis plants. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) are plant-specific Ca²⁺ sensors and effectors which mediate diverse Ca²⁺ signaling transduction pathways in plant growth, development, and stress responses. However, the functions of CBLs and CIPKs in bermudagrass (Cynodon dactylon L.), a widely planted warm-season turfgrass species with great economic value, remain poorly understood. In this study, a total of 33 CdCBL and 81 CdCIPK genes were identified in the bermudagrass genome, and were clustered in three and five groups according to their phylogenetic relationships, respectively. In line with their sequence divergence, different groups of CdCBL and CdCIPK genes exhibited different gene structures and expression patterns. Systematic yeast two-hybrid screening indicated that 27 CdCBL-CdCIPK complexes could be formed from 290 putative CdCBL and CdCIPK protein pairs. Among the CdCIPK proteins, CdCIPK29-A1 was found to interact with up to four CdCBL proteins. The CdCIPK29-A1 gene was preferentially expressed in the stolon internode of bermudagrass plants and the CdCIPK29-A1 protein was located to the cytoplasm. The expression of CdCIPK29-A1 in molecularly modified Arabidopsis thaliana (Arabidopsis) plants further indicated that CdCIPK29-A1 could regulate the stem growth angle and gravitropism possibly through modulating the starch metabolism in stem endodermal cells. These results not only established a solid foundation to explore the Ca²⁺ signaling transduction pathways in bermudagrass but also provided new insight into the function of CBL-CIPK complex in plant gravitropic response and stem growth angle regulation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"68"},"PeriodicalIF":5.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bol-miR168a is a key regulator of defense responses to Sclerotinia sclerotiorum in Brassica oleracea.","authors":"Dandan Zha, Muhammad Adnan Raza, Xiaomeng Ye, Jianghua Song","doi":"10.1007/s00299-025-03451-8","DOIUrl":"10.1007/s00299-025-03451-8","url":null,"abstract":"<p><strong>Key message: </strong>Overexpressing Bol-miR168a in cabbage reduces resistance to Sclerotinia sclerotiorum, highlighting its role in disease susceptibility regulation. miRNA-mediated post-transcriptional regulation plays a central role in regulating physiological processes, including plant growth and development, hormone signal transduction, and stress response. However, the underlying mechanism and function of microRNA-mediated repression, particularly in relation to conferring resistance to S. sclerotiorum, has not been extensively explored. In this study, Bol-miR168a was isolated from cabbage, and its function was identified in transgenic Arabidopsis and cabbage under S. sclerotiorum infection. The miR168a gene promoter contains multiple hormone response and stress response elements, which can be bound and activated by corresponding transcription factors. Expression of the Bol-miR168a was strongly induced during the first 48 h post-inoculation. The overexpression of Bol-miR168a in transgenic cabbage resulted in enhanced sensitivity to S. sclerotiorum infection, as evidenced by decreased antioxidant enzyme activities, reduced phenylalanine ammonia-lyase (PAL) content, and elevated malondialdehyde (MDA) levels. Consistent with this, Bol-miR168a overexpression in Arabidopsis (Arabidopsis thaliana) promoted plant susceptibility to S. sclerotiorum and led to increased their capacity to detoxify reactive oxygen species (ROS) in the leaves. Furthermore, in Bol-miR168a-overexpressing plants, disease-related genes displayed distinct expression patterns in transgenic Arabidopsis and cabbage, highlighting differential regulatory responses to pathogen infection. Taken together, our results suggest that Bol-miR168a played a negative role in regulating the resistance of cabbage to S. sclerotiorum, providing a basis for further investigating how Bol-miR168a regulates resistance to S. sclerotiorum.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"67"},"PeriodicalIF":5.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NIN-like proteins NLP8 negatively regulate drought resistance in Arabidopsis by regulating the expression level of PUB23.","authors":"QiJun Ma, Yu Sun, Shuo Xu, KaiJing Zuo","doi":"10.1007/s00299-025-03447-4","DOIUrl":"10.1007/s00299-025-03447-4","url":null,"abstract":"<p><strong>Key message: </strong>NLP8-mediated transcriptional activation of PUB23 expression negatively regulates drought resistance. Drought severely affects crop yields, and nitrate is essential for plant growth and development. However, the precise mechanisms by which nitrate influences drought stress responses are not fully understood. In the study, we investigated the role of the nitrate-responsive transcription factor NLP8 in drought stress. Our results showed that the nlp8 mutant exhibited enhanced drought tolerance. Transcriptome analysis revealed that NLP8 primarily regulates sugar metabolism, lipid metabolism, and nitrogen metabolism under drought conditions. Furthermore, EMSA, yeast one-hybrid, and LUC assays confirmed that NLP8 binds to the PUB23 promoter and activates its expression, thereby participating in drought resistance. The study defines the relationship between nitrate signalling and drought stress and provides insights into how plants regulate nutrient allocation during drought. These findings not only uncover the mechanisms by which plants modulate nutrient utilization under adverse conditions but also provide important insights into the physiological processes involved in plant stress responses.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 3","pages":"66"},"PeriodicalIF":5.3,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}