Plant Cell Reports最新文献

{"title":"The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.","authors":"Zhi Zou, Xiaowen Fu, Na Li, Panyan Zheng, Ruiling Wang, Hongyan Liu, Jiaquan Huang, Yongguo Zhao","doi":"10.1007/s00299-025-03534-6","DOIUrl":"10.1007/s00299-025-03534-6","url":null,"abstract":"<p><strong>Key message: </strong>A WRI-like gene (CeWRI3) homologous to AtWRI3/4 was isolated from oil-rich Cyperus esculentus tubers, which was shown to function in TAG accumulation through activating CeOLE2. In oilseeds, WRI1 has proven to be a master regulator in the transcriptional control of genes involved in fatty acid biosynthesis. By contrast, no evidence is available for WRI1 and its homologs in regulating genes associated with triacylglycerol (TAG) biosynthesis. In this study, we present the characterization of a WRINKLED (WRI)-like gene from tigernut (Cyperus esculentus L., Cyperaceae), a rare example accumulating high levels of TAGs in underground tubers. This gene was named CeWRI3 for its homology to AtWRI3/-4 in Arabidopsis thaliana. Subcellular localization analysis in Nicotiana benthamiana and transactivation assay in yeast revealed that CeWRI3 indeed functions as a transcription factor with nuclear localization and transcriptional activation activity. Further qRT-PCR analysis showed that CeWRI3 exhibits a constitutive expression pattern and its transcripts are positively correlated with TAG accumulation during tuber development. Moreover, transient overexpression of CeWRI3 in N. benthamiana leaves could significantly enhance the TAG content, implying its contribution to TAG accumulation in tigernut tubers. Correspondingly, yeast one-hybrid and dual-luciferase reporter assays revealed that CeWRI3 could activate CeOLE2, but not CeOLE5 and CeDGAT2b, another two key genes related to TAG biosynthesis. Our findings emphasize the potential application of CeWRI3 in improving oil production of vegetative tissues.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"146"},"PeriodicalIF":5.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Sodium nitroprusside modulates oxidative and nitrosative processes in Lycopersicum esculentum L. under drought stress.","authors":"Cengiz Kaya, Ferhat Uğurlar, Chandra Shekhar Seth","doi":"10.1007/s00299-025-03529-3","DOIUrl":"10.1007/s00299-025-03529-3","url":null,"abstract":"","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"145"},"PeriodicalIF":5.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plantago species are emerging model organisms for functional genomics and stress biology.","authors":"Hannah Levengood, Lillian Smith, Shelby Gillis, Yun Zhou, Cankui Zhang","doi":"10.1007/s00299-025-03530-w","DOIUrl":"10.1007/s00299-025-03530-w","url":null,"abstract":"<p><strong>Key message: </strong>Species in the Plantago genus are emerging model organisms to multiple research disciplines. The genus Plantago has long been recognized for its significance in various research fields, yet it remains underutilized as a model organism in scientific studies. Several Plantago species possess unique traits, including easily accessible vascular tissues, medicinal properties, gynodieocity, and remarkable adaptability to diverse environmental conditions. These characteristics position Plantago as a promising model for research in areas such as plant vascular biology, stress physiology, reproductive biology, ecology, and medicinal biochemistry. Recent advancements, including the development of genetic transformation systems, the availability of sequenced genomes, and the application of CRISPR-Cas9 technology, have significantly enhanced the capability of using Plantago as a model system. This review discusses the research potential of Plantago species, highlighting key historical discoveries and recent breakthroughs that demonstrate their value across multiple scientific disciplines.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"142"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A loss-of-function of ZmWRKY125 induced by CRISPR/Cas9 improves resistance against Fusarium verticillioides in maize kernels.","authors":"Letizia Ottaviani, Rozenn Lefeuvre, Emilie Montes, Thomas Widiez, Paola Giorni, Axel Mithöfer, Adriano Marocco, Alessandra Lanubile","doi":"10.1007/s00299-025-03544-4","DOIUrl":"10.1007/s00299-025-03544-4","url":null,"abstract":"<p><strong>Key message: </strong>ZmWRKY125 negatively regulates maize resistance to Fusarium verticillioides infection through modulating phytohormone, ROS scavenging and secondary metabolite gene expression as well as jasmonic and abscisic acid biosynthetic pathway activity. Fusarium verticillioides causes heavy damage to maize growth and yield and is responsible for mycotoxin contamination. Despite its widespread occurrence, few resistant genes have been identified and functionally validated for their role in the defense mechanisms against this fungus in maize. WRKY transcription factors are known to be crucial in regulating the expression of defense-responsive genes towards pathogen attack. In this context, in our previous genome-wide association study one SNP in the gene ZmWRKY125 was found significantly associated with the responses to F. verticillioides infection in maize seedlings. Here, loss-of-function mutant lines of ZmWRKY125 were obtained by the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system. The zmwrky125 edited lines were phenotypically evaluated showing a decrease by about 5 and 4 times of Fusarium ear rot (FER) severity and fumonisin contamination, respectively, compared to the wild-type genotype. The transient expression of ZmWRKY125 in maize protoplasts confirmed a nuclear localization as expected for a transcription factor. RNA-seq analysis comparison using two zmwrky125 edited lines and the wild-type genotype highlighted an enhanced modulation of the jasmonic acid (JA) and abscisic acid (ABA) hormones, redox state, cell wall modification, and secondary metabolism-associated genes after fungal infection. Moreover, the increased expression of JA- and ABA-related genes correlated with a wider accumulation of these two phytohormones in the mutant background in contrast to wild-type. This data provided new information for understanding the function of ZmWRKY125, despite further field evaluations will be required for validation of the resistance against FER.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"144"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression analyses of sweet cherry AUX/IAAs and involvements of PavAUX/IAA9/11 in regulating fruitlet abscission.","authors":"Qiandong Hou, Guang Qiao, Chunqiong Shang, Luonan Shen, Kiu Zhou, Yi Min, Xiaopeng Wen","doi":"10.1007/s00299-025-03539-1","DOIUrl":"10.1007/s00299-025-03539-1","url":null,"abstract":"<p><strong>Key message: </strong>The sweet cherry genome contains 25 AUX/IAA members, of which PavAUX/IAA9 and PavAUX/IAA11 negatively and positively regulate fruitlet abscission, respectively. The auxin/indole-3-acetic acid (AUX/IAA) gene family is plant-specific and plays various roles in growth and development. However, its function in sweet cherry fruitlet abscission remains unclear. This study identified 25 AUX/IAA members in the sweet cherry (Prunus avium L.) genome, which exhibit conserved gene structures and are subjected to purifying selection. The promoter regions of these genes contain numerous auxin response-related cis-regulatory elements. RNA-seq and RT-qPCR analyses revealed that several genes, including PavAUX/IAA9, were down-regulated, whereas PavAUX/IAA11 was up-regulated during the fruitlet abscission. Both PavAUX/IAA9 and PavAUX/IAA11 are nuclear-localized proteins, and their overexpression in Arabidopsis thaliana alters growth-related phenotypes, and delays or promotes petal abscission, respectively. Yeast two-hybrid (Y2H) and luciferase complementation imaging (LCI) assays demonstrated that PavAUX/IAA9 interacts with itself and with PavTMP87. Yeast one-hybrid and dual-luciferase reporter assays demonstrated that PavARF4 and PavWRKY40 interact with the promoters of PavAUX/IAA9 and PavAUX/IAA11, respectively. PavARF4 and PavWRKY40 lack transcriptional activation activity in yeast systems, suggesting that they function as transcriptional repressors. The regulation of AUX/IAAs expression by sweet cherry ARF and WRKY transcription factors, coupled with the interaction between AUX/IAA and transmembrane proteins (such as PavTMP87), suggests a complex auxin signaling pathway involved in fruitlet abscission in sweet cherry. This highlights the critical regulatory function of AUX/IAA proteins in plant organ abscission.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"143"},"PeriodicalIF":5.3,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Type-A and -C response regulator genes positively impact rice plant height and panicle architecture.","authors":"Chenyu Rong, Renren Zhang, Jing Xie, Jieru Li, Tiantian Yan, Ziyu Liu, Yuexin Liu, Ruihan Xu, Xi'an Shi, Xuebin Zhao, Jiali Song, Yayi Meng, Zhongyuan Chang, Yanfeng Ding, Chengqiang Ding","doi":"10.1007/s00299-025-03531-9","DOIUrl":"10.1007/s00299-025-03531-9","url":null,"abstract":"<p><strong>Key message: </strong>Thirteen type -A and two type -C RR genes exhibited distinct expression patterns and unique functions. Notably, RR2 and RR4 had the most significant positive effects impact on panicle development. Cytokinin signal transduction occurs through a \"two-component system\". Type-A and -C response regulators (RRs) are groups of proteins of similar structures constituting significant components of cytokinin signal transduction. In rice, 13 (Type-A) and 2 (Type-C) RRs have been identified to date; however, their functions remain partially known. In this study, we examined the expression patterns of Type-A and Type-C RRs in rice using RNA-Seq and confirmed their functions by constructing mutants of the 15 genes with CRISPR/Cas9. Almost all Type-A RRs played positive roles in the development of secondary branches and secondary spikelets, particularly RR2 and RR4. Notably, rr1 rr2 and rr8 rr12 rr13 higher-order mutants displayed small panicle sizes and decreased plant height. Additionally, both Type-C RRs played positive roles in regulating heading date. RNA-seq revealed several genes with significantly altered expression in the rr2 and rr4 mutants, with almost half of the differentially expressed genes (DEGs) overlapping between the two mutants. Many of the DEGs were associated with the cytokinin and abscisic acid pathways. Our findings provide new insights into the functions of Type-A and -C RRs in rice growth and may serve as a foundation for future studies focusing on cytokinin signaling.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"141"},"PeriodicalIF":5.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering ABA/PYL gene family in flax: evolutionary analysis, and abiotic stress response.","authors":"Shashank Kumar Yadav, Ankit Saroha, Devender Singh, Pragya Yadav, Dhammaprakash Pandhari Wankhede, Gyanendra Pratap Singh, Viswanathan Chinnusamy, Vikender Kaur","doi":"10.1007/s00299-025-03517-7","DOIUrl":"10.1007/s00299-025-03517-7","url":null,"abstract":"<p><strong>Key message: </strong>The identified PYL/PYR/RCAR receptor gene family differentially modulates stress-responsive and developmental roles in linseed. This gene family is evolutionarily and functionally conserved in agronomically imperative oilseed crop species. Abscisic acid (ABA) functions as a key phytohormone, exerting significant regulation over diverse cellular activities, notably growth and responses to various environmental stressors. The protonated form of ABA (ABAH) is perceived by intracellular Pyrabactin resistance 1-like (PYR/PYL) proteins, constituting an integral component of the signaling cascade. The PYLs represent one of the largest phytohormone receptor families identified. The receptors have been extensively investigated in numerous plant species. However, the PYL family in oilseed crop species has not been systematically identified at the genome level. This study identified 15 members of the PYL gene family in the linseed, exhibiting a non-uniform distribution across the 15 chromosomes. Based on structural and functional similarities, the phylogenetic analysis divided linseed PYLs (LuPYLs) into three subfamilies. Gene and motif structure analysis of LuPYLs revealed that members of each subfamily share similar gene and motif structures. An evolutionary relationship with PYL members in other oilseed crop species has been established, wherein whole-genome duplication appears to be the primary driving force underlying the expansion of the PYLs. Collinearity segregated PYL genes from all selected species into five distinct clusters, each representing a conserved functional module. The regulation of PYL genes in linseed, including transcriptional analysis of CREs in promoter regions, identification of SNPs, and assessment of miRNA-mediated post-transcriptional regulation, was assessed. Tissue-specific qRT-PCR analysis revealed diverse expression patterns of LuPYL genes, highlighting their roles in plant development and abiotic stress responses. These findings provide valuable insights into how PYL genes contribute to abiotic stress tolerance in flax.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 7","pages":"140"},"PeriodicalIF":5.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sucrose transport gene FaSWEET9a regulated by FaDOF2 transcription factor promotes sucrose accumulation in strawberry.","authors":"Yan Xu, Shuang Liu, Hongying Sun, Jian Zang, Chao Zhang, Wei Guo, Zhihong Zhang","doi":"10.1007/s00299-025-03528-4","DOIUrl":"10.1007/s00299-025-03528-4","url":null,"abstract":"<p><strong>Key message: </strong>FaSWEET9a, an important sucrose transport gene regulated by transcription factor FaDOF2, regulates the accumulation of sucrose in strawberry fruits and affects the growth of strawberry plants.. This study identified and characterized 25 members of the SWEET gene family in the genome of cultivated strawberry (Fragaria × ananassa cv. 'Yanli'), focusing on their potential roles in fruit development. Notably, FaSWEET9a, a specific member of the SWEET family, was found to be uniquely expressed in 'Yanli' fruit. Functional analysis via heterologous expression in Saccharomyces cerevisiae confirmed that FaSWEET9a acts as a sucrose transporter. To further investigate its role, we generated FaSWEET9a overexpression lines and demonstrated that FaSWEET9a not only enhances sucrose accumulation in strawberry fruits but also influences plant growth and development. We identified FaDOF2 that could bind to the promoter of FaSWEET9a and enhance its transcription by conducting yeast one-hybrid assays, electrophoretic mobility shift assays, β-glucuronidase assays, and luciferase reporter gene assays. Moreover, transient transformation experiments revealed that FaDOF2 could elevate sucrose content in strawberry fruits by regulating FaSWEET9a. This research brings new viewpoints on the molecular mechanisms that govern sucrose regulation in strawberry fruits, spotlighting the functional significance of the FaSWEET9a-FaDOF2 regulatory module in the aspects of fruit quality and development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"138"},"PeriodicalIF":5.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced thermotolerance via overexpression of a stromal ascorbate peroxidase in Nannochloropsis oceanica.","authors":"Shan Wu, Yiting She, Xitao Chen, Xingwei Huang, Tao Xu, Congcong Miao, Qianyi Wu, Zheng Li, Chengcheng Li, Yuhui Cheng, Yi Xin","doi":"10.1007/s00299-025-03511-z","DOIUrl":"10.1007/s00299-025-03511-z","url":null,"abstract":"<p><strong>Key message: </strong>Overexpression of the heat-responsive ascorbate peroxidase gene NoAPX2430 improves thermotolerance and growth of Nannochloropsis oceanica, offering potential strategies for enhanced resilience in plant cells. Microalgae are promising for industrial lipid production but face challenges from high-temperature (HT) stress. This study focused on improving thermotolerance in Nannochloropsis oceanica by studying and modifying the heat-responsive ascorbate peroxidase gene, NoAPX2430. Functional and localization analyses confirmed NoAPX2430 as an active enzyme in the chloroplast stroma. Knockdown of NoAPX2430 resulted in reduced growth, photosynthetic efficiency, and reactive oxygen species (ROS) scavenging under HT (35 °C). In contrast, overexpression of NoAPX2430 significantly improved photosynthetic performance, lowered ROS levels, and boosted growth rates. Additionally, lipid and triacylglycerol contents increased significantly under combined nitrogen depletion and HT, with polyunsaturated fatty acids rising by up to 194.4%. These results highlight NoAPX2430's critical role in enhancing thermotolerance and lipid biosynthesis. This provides a foundation for genetic and environmental strategies to boost microalgal resilience and productivity for sustainable biofuel and nutraceutical development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"139"},"PeriodicalIF":5.3,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual regulation of seed coat structure and flavonoids biosynthesis on seed dormancy formation of alfalfa.","authors":"Shushuan Wang, Yutong Zhang, Ruru Shi, Wenqiang Fan, Fengling Shi","doi":"10.1007/s00299-025-03526-6","DOIUrl":"10.1007/s00299-025-03526-6","url":null,"abstract":"<p><strong>Key message: </strong>The metabolic regulation of flavonoids and GAs reinforces the defensive function of the seed coat, thereby establishing and maintaining dormancy. Medicago falcata L. is an important genetic resource for stress resistance in legumes with high seed value. However, its hard seed coat causes low germination rates, uneven seedling emergence, and prolonged dormancy. Moreover, the contributions of seed coat structure and secondary metabolites, including flavonoids and phytohormones, to dormancy formation in Medicago falcata L. seeds remain unknown. This study investigated structural changes in the seed coat surface and internal layers, along with accumulation patterns of flavonoids and phytohormone biosynthesis/catabolism. Results showed that hard seeds had extremely low germination rates, rough seed coat surfaces, reduced hilum aperture size, and densely arranged internal palisade layers with maximum thickness, confirming the decisive role of seed coat structure (especially the palisade layer) in dormancy. Flavonoids, such as flavonols and isoflavonoids (liquiritigenin, myricetin, daidzein, 3-O-methylquercetin, and kaempferol-3-O-galactoside), were significantly upregulated in hard seeds. Notably, anthocyanin and flavonol biosynthesis share the common precursor aromadendrin, which was preferentially allocated toward flavonol production, leading to the downregulation of anthocyanin precursors (dihydroquercetin, afzelechin, epiafzelechin). Gibberellins (GA4, GA29, GA34) were significantly downregulated in hard seeds, while elevated ABA/GAs and ABA/JA ratios promoted dormancy formation. This study reveals changes in seed coat texture, internal cell layer arrangement, and palisade layer thickness during hard seed development, while highlighting the core regulatory roles of flavonoids and GAs in dormancy establishment.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"137"},"PeriodicalIF":5.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}