Plant Cell Reports最新文献

{"title":"Mapping stress memory: genetic and epigenetic insights into combined drought and heat tolerance in barley.","authors":"Amr Elkelish, Ahmad M Alqudah, Abdulrahman M Alhudhaibi, Hussain Alqahtani, Essa M Saied, Andreas Börner, Samar G Thabet","doi":"10.1007/s00299-025-03501-1","DOIUrl":"10.1007/s00299-025-03501-1","url":null,"abstract":"<p><strong>Key message: </strong>Unveiling genetic and epigenetic mechanisms in barley, this study maps stress memory under combined drought and heat, advancing resilience breeding for climate-adaptive crop improvement. Barley is one of the world's most important cereal crops and is increasingly threatened by concurrent drought and heat stress, two major environmental factors intensified by climate change. In our study, we employed a genome-wide association scan (GWAS) to investigate the concept of \"stress memory,\" wherein barley plants exposed to previous stress events exhibit enhanced responses to subsequent ones. We evaluated key agronomic traits, such as plant height, spike length, grain number, and thousand kernel weight along with biochemical markers such as chlorophyll content, proline, and soluble proteins across three generations under combined drought and heat stress. This approach encompassed transgenerational and intergenerational stress memory and a third generation that could reveal the potential cumulative effects of combined drought and heat stress. Our findings demonstrated a significant increase in metabolites specifically proline and soluble proteins in third-generation barley plants compared to those exposed to stress for only one or two generations. Through GWAS analysis, we identified 332 highly significant SNP markers clustered within 14 genomic regions on chromosomes 2H, 3H, 4H, 5H, and 7H. These regions are associated with all evaluated physiological and morphological traits under stress that harbor several potential candidate genes implicated in regulating complex signaling pathways, reactive oxygen species scavenging, and energy metabolism processes essential for mitigating the impacts of drought and heat. These results underscore the intricate nature of barley's stress tolerance mechanisms and highlight the potential for integrating genomics, epigenomics, and advanced phenotyping approaches into breeding programs.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"120"},"PeriodicalIF":5.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates plant stem cell maintenance under oxidative stress.","authors":"Jiaqi Qiu, Minghuang Chen, Zheqi Cai, Xiaofen Chen, Zelong Pang, Hao Chen, Tao Huang","doi":"10.1007/s00299-025-03507-9","DOIUrl":"10.1007/s00299-025-03507-9","url":null,"abstract":"<p><strong>Key message: </strong>GAPDH regulates plant stem cell maintenance. WUSCHEL (WUS) and WUSCHEL-RELATED HOMEOBOX (WOX) family proteins are vital for maintaining the homeostasis of stem cells, which is necessary for the continuous growth and the development of plants. Plants frequently encounter environmental stress that can lead to an increase in reactive oxygen species, such as hydrogen peroxide (H₂O₂). However, the exact ways in which plant stem cells sense and respond to H₂O₂ signals remain unclear. This research indicates that cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) helps regulate stem cell maintenance in Arabidopsis in response to H₂O₂. Hydrogen peroxide causes the relocation of two cytosolic GAPDH proteins, GAPC1 and GAPC2, from the cytoplasm to the nucleus. These isoforms interact with WUS/WOX proteins and modulate the expression of the WUS/WOX gene by binding to its promoter. When the expression of GAPC1 and GAPC2 is decreased, stem cell homeostasis and overall plant growth become more sensitive to H₂O₂. Thus, cytosolic GAPDH may serve as a sensor for H₂O₂, influencing the maintenance of plant stem cells under oxidative stress.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"121"},"PeriodicalIF":5.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-based modulation of V-PPase expression in rice improves grain quality and yield under high nighttime temperature.","authors":"Flávia Barbosa Silva Botelho, Soumen Nandy, Vibha Srivastava","doi":"10.1007/s00299-025-03504-y","DOIUrl":"10.1007/s00299-025-03504-y","url":null,"abstract":"<p><strong>Key message: </strong>Transcriptional modulation of the vacuolar H⁺ translocating pyrophosphatase expressed specifically in the endosperm and reproductive tissue of rice improves its spikelet fertility and reduces grain chalkiness under high nighttime temperature.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"119"},"PeriodicalIF":5.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SlDCD and SlLCD increased the salt tolerance in tomato seedlings by enhancing antioxidant and photosynthesis capacity.","authors":"Xinfang Chen, Dengjing Huang, Xiaoling Man, Ailing Li, Hua Fang, Siting Lu, Di Yang, Weibiao Liao","doi":"10.1007/s00299-025-03509-7","DOIUrl":"10.1007/s00299-025-03509-7","url":null,"abstract":"<p><strong>Key message: </strong>Using gene silence and heterologously overexpression, hydrogen sulfide synthesis-related genes l-cysteine desulfhydrase and d-cysteine desulfhydrase have been shown to enhance salt tolerance in tomato seedlings. Hydrogen sulfide (H₂S) plays an important role in alleviating abiotic stress. L-Cysteine desulfhydrase (LCD) and D-cysteine desulfhydrase (DCD) are two important H₂S synthesis enzymes. Until now, whether and how SlDCD and SlLCD increase salt tolerance in plant are still unknown. Here, we explored the effects of SlDCD and SlLCD on salt tolerance in tomato seedlings by silencing SlDCD and SlLCD and heterologously overexpressing SlDCD and SlLCD. In tomato seedlings, exogenous sodium hydrosulfide (NaHS, a H₂S donor) increased salt tolerance while decreasing H₂S synthesis-related enzyme activity, endogenous H₂S levels, and H₂S synthesis-related gene expression. Silencing SlDCD and SlLCD inhibited tomato seedling growth under salt stress, increased relative conductivity, MDA, H₂O₂, O₂^-, Pro, and carotenoid content, Ci and NPQ. In contrast, it decreased the activity of antioxidant enzymes (POD, SOD, CAT and APX) and the expression of related genes (POD, SOD, CAT and APX), chlorophyll content, photosynthetic parameters (Pn, Gs and Tr) and fluorescence parameters (Fv/Fm, φPSII and qP), while exogenous NaHS considerably mitigated the adverse impacts of salt stress in SlDCD and SlLCD silenced-tomato seedlings. Overexpression of SlDCD and SlLCD in Arabidopsis significantly enhanced plant salt tolerance. Taken together, our results indicate that SlDCD and SlLCD could enhance the antioxidant activity and photosynthesis capacity under salt stress, which results improving salt tolerance in tomato seedlings.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"117"},"PeriodicalIF":5.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for maintaining the release of co-suppression and maximally restoring the RDR6 expression.","authors":"Na Li, Hailan Wang, Jiayin Wen, Xiangling Liu, Meng Zhang","doi":"10.1007/s00299-025-03508-8","DOIUrl":"10.1007/s00299-025-03508-8","url":null,"abstract":"<p><strong>Key message: </strong>Tissue-specific RDR6 compensation rescues Arabidopsis defects while maintaining seed polyunsaturated fatty acid accumulation, balancing co-suppression relief and key trait retention for modular engineering. The transgene-induced co-suppression of fatty acid desaturase 2 (FAD2) can be effectively released in rdr6 mutant, enabling a significant increase in polyunsaturated fatty acid (PUFA) content in seeds. However, the global suppression of RNA-dependent RNA polymerase 6 (RDR6) compromises plant growth and disease resistance. To address this limitation, we developed a spatiotemporal compensation strategy by restoring RDR6 expression in non-seed tissues using tissue-specific promoters while maintaining its low expression during seed maturation. To implement this goal, we identified P_BnTC06, a Brassica napus promoter, through transcriptomic data mining and functional characterization. GUS staining revealed that the P_BnTC06 promoter drives strong gene expression in vegetative tissues (e.g., leaves, stems, and flowers) but exhibits negligible activity in mid- to late-stage-developing seeds. We introduced P_BnTC06::RDR6 into Pha::AtFAD2/rdr6-11, the previously established high- PUFA Arabidopsis line. This intervention rescued the rdr6 mutant phenotype (characterized by gracile, downward-curling leaves) to wild-type morphology and restored RDR6 expression across non-seed tissues, while maintaining minimal expression in middle and late developing seeds. Crucially, FAD2 transcript levels remained at a high level during late seed development, resulting in sustained high PUFA accumulation in mature seeds. This strategy establishes a practical strategy to circumvent transgene co-suppression and proposes a modular framework for precision breeding of complex traits in crops.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"118"},"PeriodicalIF":5.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144042013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidates for regulating cytosolic Ca²⁺ influx during inducible aerenchyma formation under low-oxygen conditions.","authors":"Li Jingxia, Mikio Nakazono, Takaki Yamauchi","doi":"10.1007/s00299-025-03497-8","DOIUrl":"10.1007/s00299-025-03497-8","url":null,"abstract":"<p><strong>Key message: </strong>The conformational change of CDPKs stimulated by cytosolic Ca²⁺ influx is essential for activating RBOH-mediated ROS production. CNGCs are promising candidates for regulating cytosolic Ca²⁺ influx during ROS-dependent aerenchyma formation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"116"},"PeriodicalIF":5.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.","authors":"Suzuka Kikuchi, Takumi Kotaka, Yuga Hanaki, Minako Ueda, Takumi Higaki","doi":"10.1007/s00299-025-03498-7","DOIUrl":"10.1007/s00299-025-03498-7","url":null,"abstract":"<p><strong>Key message: </strong>Using deep learning-based image restoration, we achieved high-resolution 4D imaging with minimal photodamage, revealing distinct localization and suggesting Lifeact-RFP-labeled actin microfilaments play a role in initiating cell plate formation. Phragmoplasts are plant-specific intracellular structures composed of microtubules, actin microfilaments (AFs), membranes, and associated proteins. Importantly, they are involved in the formation and the expansion of cell plates that partition daughter cells during cell division. While previous studies have revealed the important role of cytoskeletal dynamics in the proper functioning of the phragmoplast, the localization and the role of AFs in the initial phase of cell plate formation remain controversial. Here, we used deep learning-based image restoration to achieve high-resolution 4D imaging with minimal laser-induced damage, enabling us to investigate the dynamics of AFs during the initial phase of cell plate formation in transgenic tobacco BY-2 cells labeled with Lifeact-RFP or RFP-ABD2 (actin-binding domain 2). This computational approach overcame the limitation of conventional imaging, namely laser-induced photobleaching and phototoxicity. The restored images indicated that RFP-ABD2-labeled AFs were predominantly localized near the daughter nucleus, whereas Lifeact-RFP-labeled AFs were found not only near the daughter nucleus but also around the initial cell plate. These findings, validated by imaging with a long exposure time, highlight distinct localization patterns between the two AF probes and suggest that Lifeact-RFP-labeled AFs play a role in initiating cell plate formation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"115"},"PeriodicalIF":5.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative multi-transcriptomic analysis uncovers core genes and potential defense mechanisms in rice-Magnoporthe oryzae interaction.","authors":"Fatma Salem, Ahmed ElGamal, Zujian Zhang, Weiwen Kong","doi":"10.1007/s00299-025-03490-1","DOIUrl":"10.1007/s00299-025-03490-1","url":null,"abstract":"<p><strong>Key message: </strong>Multiple transcriptomic comprehensive analyses highlight key genes and cast new light on multifaceted pathways that may be important arenas in rice innate immunity against Magnoporthe oryzae blast disease. Magnaporthe oryzae (MOR) poses a significant threat to rice production worldwide. However, defense mechanisms in rice against MOR remain inadequately defined. In this study, a multi-transcriptomic integrative analysis on 441 samples from diverse microarrays and RNA-seq sets was conducted to reveal critical factors in rice defense against MOR infection. A robust pattern of 3534 upregulated genes and 2920 repressed genes was commonly identified across all MOR-infected arrays and RNA-seq profiles. Interestingly, enrichment analysis revealed a consistent triggering of endoplasmic reticulum (ER)-related mechanisms and citric acid cycle (TCA) influx in rice response to MOR infection across all the transcriptome profiles, suggesting their critical role in modulating rice immunity against the pathogen. By contrast, chloroplast and photosynthesis pathways were frequently repressed across all the profiles. Among ER-related mechanisms, the phagosome pathway involved in the activation of NADPH oxidase was highly triggered in early response to MOR infection. Moreover, WGCNA analysis highlighted four key co-expressed gene modules and 80 significant hub genes associated with MOR infection. Among the core genes, Sec61 gene involved in the ER-translocation process was identified along with OsMFP (peroxisomal oxidation gene) and OSAHH gene (involved in cyclic-trans-methylation). Furthermore, MPK6, WRKY24, NUP35, and NPR1 genes were observed as core co-expressed genes, suggesting their significance in regulating rice immunity against MOR. Our findings elucidate key genes and multifaceted mechanisms in rice-MOR interaction, proposing new informative clues that can be exploited to improve rice resistance against blast disease.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"114"},"PeriodicalIF":5.3,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blooming resilience: transcriptomic insights into cotton flower responses to boll weevil infestation.","authors":"Sarah Muniz Nardeli, Ana Luiza Atella de Freitas, Luis Willian Pacheco Arge, Leonardo Lima Pepino Macedo, Marcelo Ribeiro-Alves, Régis Lopes Corrêa, Maria Fatima Grossi-de-Sa, Marcio Alves-Ferreira","doi":"10.1007/s00299-025-03503-z","DOIUrl":"10.1007/s00299-025-03503-z","url":null,"abstract":"<p><strong>Key message: </strong>Cotton plants undergo a drastic transcriptional reprogramming after cotton boll weevil infestation, modulating several defense pathways to cope with the damage. The global demand for cotton fiber continues to rise, but pests and pathogens significantly hinder cotton production, causing substantial losses. Among these, the cotton boll weevil (Anthonomus grandis) is one of the most destructive pests. To investigate the molecular responses of cotton (Gossypium hirsutum) to boll weevil infestation, we evaluated the global gene expression of floral buds using mRNA-seq. Additionally, we analyzed the expression of non-coding RNAs, including microRNAs (miRNAs) and long intergenic non-coding RNAs (lincRNAs). Infestation by cotton boll weevil larvae triggered a rapid and drastic transcriptional reprogramming, with 1,656 and 1.698 genes modulated after two and twelve hours, respectively. Gene ontology enrichment analysis revealed significant regulation of defense-related and developmental processes, including photosynthesis, primary metabolism, and cell organization. Transcription factor families such as ERF, WRKY, GRAS, and NAC were strongly affected, highlighting their roles in coordinating defense responses. The jasmonate pathway showed intensive modulation, alongside secondary metabolite pathways like terpenoids and phenylpropanoids, which contribute to plant defense mechanisms. Non-coding RNAs also played a critical role in the response. We identified 921 unique known and novel miRNAs, with 36 modulated by the infestation, and predicted 98,850 putative lincRNAs, several of which were differentially expressed. Understanding the genetic and molecular mechanisms underlying cotton's defense against boll weevil, particularly during early infestation stages, is vital for developing biotechnological strategies to reduce pest damage. Our findings provide critical insights to enhance cotton resilience against herbivores.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 6","pages":"113"},"PeriodicalIF":5.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional analysis of the woody oil crop Plukenetia volubilis L. LEC2 homolog PvoB3-69 in promoting regeneration.","authors":"Jing-Jing Yu, Shiling Deng, Jinhui Mo, Han Huang, Zeng-Fu Xu, Yi Wang","doi":"10.1007/s00299-025-03493-y","DOIUrl":"https://doi.org/10.1007/s00299-025-03493-y","url":null,"abstract":"<p><strong>Key message: </strong>Identified a robust regeneration-related genomic sequence of PvoB3-69 from the B3 superfamily in Plukenetia volubilis. gPvoB3-69-assisted transformation of P. volubilis obtained transgenic shoots for the first time. The regenerative capacity of host cells is critical for the genetic transformation efficiency of woody plants. The B3 superfamily  is particularly involved in early embryo morphogenesis and late-stage embryo maturation. In this study, 74 PvoB3 members were identified in the genome of P. volubilis, and classified into four subfamilies: LAV, RAV, ARF, and REM. RNA-seq and RT-qPCR analyses revealed that PvoB3-69, a member of the LAV subfamily, has specific expression patterns similar with LEC2. Overexpression of gPvoB3-69 enhanced the bud regeneration capacity of transgenic Nicotiana benthamiana organs during in vitro culture. Additionally, overexpression of gPvoB3-69 significantly improved somatic embryogenesis in transgenic Arabidopsis plants, especially with the aid of 2,4-D. Transcriptome analysis in Arabidopsis thaliana revealed that PvoB3-69 may enhance somatic embryo induction efficiency by activating developmental regulators such as WUS and LEC1, while also modulating salicylic acid, ABA, and ethylene metabolism. Furthermore, overexpression of gPvoB3-69 in the cotyledons of P. volubilis increased the regeneration ability of host cells, and broke the genetic transformation barrier through the Agrobacterium-mediated method, allowing the regenerated transgenic shoots obtained for the first time. This study provides the first systematic analysis of the B3 superfamily in P. volubilis, identifying PvoB3-69 as a key regulator of regeneration. These findings establish a foundation for further comprehensive studies of PvoB3 genes and deepen our understanding of the regulatory mechanism of shoot regeneration in P. volubilis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 5","pages":"112"},"PeriodicalIF":5.3,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}