Plant Cell Reports最新文献

{"title":"Combining resistance indicators, metabolomes and transcriptomes to reveal correlations in disease and cold resistance in tea plant and analyze the key domain NB-ARC.","authors":"Min Li, Wenting Wang, Xiaodan Chen, Xiumei Lu, Yahui Huang","doi":"10.1007/s00299-024-03384-8","DOIUrl":"10.1007/s00299-024-03384-8","url":null,"abstract":"<p><strong>Key message: </strong>Integration of resistance indicators, metabolomes, and transcriptomes to elucidate that there is a positive correlation between disease susceptibility and cold tolerance in tea plants. The flavonoid pathway was found to be the major metabolic and transcriptional enrichment pathway. A key domain NB-ARC was identified through joint analysis, along with analysis of key domains within the NB-ARC protein. Tea is a healthy beverage and the tea plant is a woody plant rich in secondary metabolites. In the face of abnormal climate change year by year, it is important to investigate the mechanisms by which tea plants resist both biotic and abiotic stresses. In this study, we found different tea plant cultivars were evaluated for cold and disease resistance have highly correlated. Subsequently, two cold and fungal resistant cultivars were screened from a Shuixian population that had been cold domesticated for 50 years, and transcriptome and metabolome assays were performed on the two materials under cold and anthracnose stresses, using Baiye Dancong as a control. The analyses found that differential metabolites were most enriched in the flavonoid pathway and differentially expressed genes were most enriched in the pathway related to disease course after pathogen stress and cold stress. Combined metabolome and transcriptome analyses identified 30 genes that were positively correlated with flavonoid content after pathogen stress and cold stress, of which the number of genes with NB-ARC structural domains was 11, which accounted for the largest proportion. These 11 genes with NB-ARC structural domains were analyzed by family analysis and found to be highly involved in different tissues transcriptomes of tea plants, indicating the importance of the NB-ARC structural domains in biotic and abiotic stresses, and providing a theoretical basis of analysis for the subsequent related studies. In this study, through the identification of resistance in different varieties of tea plant and the multi-omics approach, we found the genes related to the key structural domain NB-ARC, which lays the foundation for the study of biologically and abiologically important mechanisms in response to the disease in tea plant.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"34"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genomic analysis of SmbHLH genes and the role of SmbHLH93 in eggplant anthocyanin biosynthesis.","authors":"XinJin Yu, SiYu Cao, JinDi Wang, DaLu Li, YongJun He","doi":"10.1007/s00299-025-03429-6","DOIUrl":"10.1007/s00299-025-03429-6","url":null,"abstract":"<p><strong>Key message: </strong>SmbHLH93can activate the expression of SmCHS, SmANS, SmDFR and SmF3H.Overexpression of SmbHLH93promotes anthocyanin biosynthesis. SmbHLH93can interact with SmMYB1 to promote anthocyanin accumulation. As an outstanding source of anthocyanins, eggplant (Solanum melongena L.) is extremely beneficial for human health. In the process of anthocyanin biosynthesis in eggplant, the basic helix-loop-helix (bHLH) transcription factor family plays a crucial role. However, the bHLH gene family is extensive, making it difficult to systematically screen and analyze their functions using conventional methods. We studied the phylogeny, gene structure, conserved motifs, promoter element, and chromosomal location of the 166 SmbHLH genes in the recently released eggplant genome. Through the analysis of transcriptomic data of eggplant peel treated with light, it was found that SmbHLH93 was the most responsive to light among those of unknown function. Additionally, it was discovered that SmbHLH93 plays a positive regulatory role in anthocyanin synthesis through dual-luciferase reporter assay(dual-LUC) and genetic transformation in Arabidopsis (Arabidopsis thaliana). Furthermore, experiments involving yeast two-hybrid (Y2H), luciferase complementation assay (Split-LUC), and tobacco transient transformation demonstrated that SmbHLH93 has the ability to interact with SmMYB1 in order to enhance anthocyanin accumulation. This study will serve as a foundation for exploring the role of SmbHLH transcription factors in anthocyanin biosynthesis in the future.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"36"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.","authors":"Abhishek Bohra, Abha Tiwari, Shalini Pareek, Rohit Joshi, S J Satheesh Naik, Khushbu Kumari, Ram Lakhan Verma, Ashok K Parihar, Prakash G Patil, Girish P Dixit","doi":"10.1007/s00299-024-03414-5","DOIUrl":"10.1007/s00299-024-03414-5","url":null,"abstract":"<p><p>Plant breeding needs to embrace genetic innovations to ensure stability in crop yields under fluctuating climatic conditions. Development of commercial hybrid varieties has proven to be a sustainable and economical alternative to deliver superior yield, quality and resistance with uniformity in a number of food crops. Cytoplasmic male sterility (CMS), a maternally inherited inability to produce functional pollen, facilitates a three-line system for efficient hybrid seed production strategies in crops. The CMS system has illustrated its potential as a robust pollination control mechanism to support the billion-dollar seed industry. In plants, CMS arises due to a genomic conflict between mitochondrial open reading frames (orfs) and nuclear-encoding restoration-of-fertility (Rf) genes, leading to floral abnormalities and pollen sterility. Research on pollen sterility and fertility restoration provides deeper insights into cytoplasmic-nuclear interplay in plants and elucidates key molecular targets for hybrid breeding in crops. More recently, programmable gene editing (e.g., TALEN, CRISPR-Cas) has emerged as a promising tool to functionally validate CMS and Rf genes and obviate the need for pollen donors or Rf-genes for hybrid breeding. Modern genomic prediction models have allowed establishment of high-performing heterotic groups and patterns for sustaining long-term gain in hybrid breeding. This article reviews latest discoveries elucidating the molecular mechanisms behind CMS and fertility restoration in plants. We then present our perspective on how evolving genetic technologies are contributing to advance fundamental knowledge of the CMS-Rf genetic system for producing crop hybrids with high heterosis.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"33"},"PeriodicalIF":5.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and functional characterization of CLG family genes reveal likely roles in epidermal development in Arabidopsis.","authors":"Qiuli Zhang, Tao Hong, Tian Su, Qiaolong Li, Aoni Xiang, Xinlong Chen, Guanghua He, Fangming Zhao, Renhong Wu","doi":"10.1007/s00299-024-03421-6","DOIUrl":"10.1007/s00299-024-03421-6","url":null,"abstract":"<p><strong>Key message: </strong>We identified a CX₂CX₁₂CPXC motif and 11 CLG genes that regulate epidermal development by interacting with homeodomain leucine-zipper IV family proteins in Arabidopsis. Zinc finger proteins (ZFPs), the key regulators of plant growth and development, can be categorized based on the sequence patterns of zinc finger motifs. Here, by aligning the amino acid sequences of CFL1, AtCFL1, AtCFL2, GIRl, and GIR2, we identified the CX₂CX₁₂CPXC motif in their C-terminus, which differs from all the previously characterized canonical zinc finger motifs. A total of 11 non-canonical ZFPs were identified in Arabidopsis based on the pattern and we named these genes as the CLG family genes (CFL1-like genes). We further established that the family genes are randomly distributed on five chromosomes, containing two conserved motifs and possess various cis-acting elements associated with plant hormones, stress responses and tissue regulation. Moreover, the family genes exhibit a wide range of expression profiles, and all of these proteins have nuclear localization signals. They may affect epidermal development through interactions with homeodomain leucine-zipper IV (HD-ZIP IV) family proteins. Overall, these findings comprehensively characterize CLG family genes and lay a foundation for further elaborating their biological functions in plant growth and development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"32"},"PeriodicalIF":5.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen availability modulates carotene biosynthesis, chromoplast biogenesis, and cell wall composition in carrot callus.","authors":"Tomasz Oleszkiewicz, Katarzyna Sala-Cholewa, Kamila Godel-Jędrychowska, Ewa Kurczynska, Anna Kostecka-Gugała, Przemysław Petryszak, Rafal Baranski","doi":"10.1007/s00299-024-03420-7","DOIUrl":"10.1007/s00299-024-03420-7","url":null,"abstract":"<p><strong>Key message: </strong>Carrot callus grown on a medium with increased nitrogen have reduced carotenoid accumulation, changed gene expression, high amount of vesicular plastids and altered cell wall composition. Carotenoid biosynthesis is vital for plant development and quality, yet its regulation under varying nutrient conditions remains unclear. To explore the effects of nitrogen (N) availability, we used carrot (Daucus carota L.) model callus cultures in vitro as a controlled system for studying nutrient-regulated metabolic processes. Two mineral media differing in N content and NO₃⁻/NH₄⁺ ratios were used. Comprehensive analyses, HPLC, transmission electron microscopy, immunochemistry, and RNA sequencing, revealed notable cellular and molecular responses to N treatments. The results demonstrated that N supplementation reduced carotenoid content by 50%, particularly β-carotene and α-carotene. The composition of chromoplast types shifted, with vesicular chromoplasts dominating (55%), followed by a globular type (23%), while in the control callus, globular and crystalline types predominated (57% and 33%, respectively). Immunohistochemistry showed increased presence of high-esterified pectins and arabinogalactan proteins in N-treated cells. Transcriptomic analysis identified 1704 differentially expressed genes (DEGs), including only two in the carotenoid biosynthesis pathway: phytoene synthase 2 (PSY2) and zeaxanthin epoxidase (ZEP). PSY2, which encodes the carotenoid rate-limiting enzyme, showed expression levels that corresponded with reduced carotene content. Other DEGs included 15 involved in nitrogen transport, 1 in nitrogen assimilation, 40 in cell wall biosynthesis and modification, and 9 in phenylpropanoid/flavonoid pathways. N-treated callus exhibited altered expression of MADS-box, NLP, bZIP, and ethylene-responsive transcription factors. These findings reveal how nitrogen availability disrupts carotenoid biosynthesis and triggers extensive chromoplast and cell wall remodeling, providing a cellular framework for understanding nutrient-regulated metabolic shifts.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"31"},"PeriodicalIF":5.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143010218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The petunia heavy metal P-type ATPase PhHMA5II1 interacts with copper chaperons and regulate Cu detoxification.","authors":"Liru Pan, Ruiling Li, Jinglei Wu, Yanbang Li","doi":"10.1007/s00299-024-03387-5","DOIUrl":"10.1007/s00299-024-03387-5","url":null,"abstract":"<p><strong>Key message: </strong>An endoplasmic reticulum-localized Cu transporter, PhHMA5II1, interacts with copper chaperones and plays an important role in Cu detoxification in petunia. Copper (Cu) is an essential element for plant growth but toxic when present in excess. In this study we present the functional characterization of a petunia (Petunia hybrida) P_1B-type heavy-metal ATPases (HMAs), PhHMA5II1. Heterologous expression of PhHMA5II1 in yeast (Saccharomyces cerevisiae) showed Cu transport activity. The expression of PhHMA5II1 in roots and shoots was unaffected by excess Cu. CRISPR/Cas9-edited mutant lines and PhHMA5II1 overexpressing transgenic plants were generated to investigate the functions of PhHMA5II1 in petunia. The PhHMA5II1 knockout mutant was hypersensitive to excess Cu and accumulated more Cu in roots compared to wild-type petunia. Overexpression of PhHMA5II1 enhanced Cu tolerance and reduced Cu accumulation in roots. Furthermore, PhHMA5II1 localized in endoplasmic reticulum, and the localization was unaffected by excess Cu. Yeast two-hybrid experiments and bimolecular fluorescence complementation assays demonstrate that PhHMA5II1 interact with petunia copper chaperons, PhATX1 and PhCCH. Finally, RNA-sequencing revealed that knockout PhHMA5II1 affected the expression of genes involved in cell-wall organization, copper ion homeostasis, and response to oxidative stress. Taken together, PhHMA5II1 plays an important role in Cu detoxification in petunia.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"29"},"PeriodicalIF":5.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of Cannabis glandular trichome development reveals distinct features of cannabinoid biosynthesis.","authors":"Matthew Nolan, Qi Guo, Lei Liu, Nicolas Dimopoulos, Lennard Garcia-de Heer, Bronwyn J Barkla, Tobias Kretzschmar","doi":"10.1007/s00299-024-03410-9","DOIUrl":"10.1007/s00299-024-03410-9","url":null,"abstract":"<p><strong>Key message: </strong>Cannabis trichome development progresses in distinct phases that underpin the dynamic biosynthesis of cannabinoids and terpenes. This study investigates the molecular mechanisms underlying cannabinoid and terpenoid biosynthesis in glandular trichomes of Cannabis sativa (CsGTs) throughout their development. Female Cannabis sativa c. Hindu Kush were cultivated under controlled conditions, and trichome development was analysed from week 3 to week 8 of the flowering period. We employed light microscopy, quantitative metabolomics and proteomics to analyse morphological changes in trichome secretory cell development, and temporal changes in metabolite accumulation and protein abundance. Our findings identified three distinct developmental phases: pre-secretory (T3), secretory (T6), and post-secretory (T8), the first time the three phases of trichome development have been identified and investigated in CsGTs. The pre-secretory phase was characterized by smaller secretory cells, limited metabolite accumulation and elevated levels of proteins involved in protein biosynthesis and cellular development. The secretory phase exhibited the highest biosynthetic activity, marked by larger secretory cells, increased plastidal activity, central carbon metabolism, and significant accumulation of cannabinoids and terpenoids. The post-secretory phase showed a decrease in secretory cell size, reduced metabolic activity, and a decrease in the abundance of primary and secondary metabolism enzymes, although THCA continued to accumulate. Key enzymes showed dynamic changes correlating with the stages of trichome development. This study provides a comprehensive understanding of the molecular mechanisms regulating cannabinoid and terpenoid biosynthesis in CsGTs, offering insights for enhancing the production of these valuable compounds through targeted breeding and biotechnological approaches.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"30"},"PeriodicalIF":5.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Cas9-targeted mutagenesis mini-library in the upland cotton genetic standard line (TM-1).","authors":"Yunfei Hao, Jiajia Feng, Yuanyuan Li, Zhangcheng Yuan, Pengfei Miao, Hailiang Cheng, Qiaolian Wang, Youping Zhang, Limin Lv, Dongyun Zuo, Ji Liu, Guoli Song","doi":"10.1007/s00299-024-03416-3","DOIUrl":"10.1007/s00299-024-03416-3","url":null,"abstract":"","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"28"},"PeriodicalIF":5.3,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cotton GhMAX2 promotes single-celled fiber elongation by releasing the GhS1FA-mediated inhibition of fatty acid biosynthesis.","authors":"Yaru Sun, Shuangxia Jin, Guoli Song","doi":"10.1007/s00299-024-03422-5","DOIUrl":"10.1007/s00299-024-03422-5","url":null,"abstract":"<p><strong>Key message: </strong>Cotton GhMAX2 positively regulates fiber elongation by mediating the degradation of GhS1FA, which transcriptionally represses GhKCS9 expression. Strigolactones (SLs) are known to promote cotton fiber development. However, the precise molecular relationship between SL signaling and fiber cell elongation remains unclear. In this study, we investigate the role of F-box E3 ligase MORE AXILLARY GROWTH2 (MAX2) in upland cotton in relation to the regulation of fiber development. GhMAX2b and GhMAX2f act as key components for SL signal transduction, with their loss-of-function leading to a notable reduction in fiber length. Biochemical analysis showed that GhMAX2b/f trigger the ubiquitination and subsequent degradation of the transcription repressor strigolactone-1-factor-At (GhS1FA), which function as a substrate for these E3 ligases. Furthermore, GhS1FA inhibits fatty acids biosynthesis by directly binding to the W-box element within the promoter of 3-ketoacyl-CoA synthases 9 (GhKCS9) and repressing its expression. In summary, we propose that GhMAX2b/f promote fiber elongation, potentially operating partially independently of GhD53 degradation.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"26"},"PeriodicalIF":5.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142953356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of the general transcription factor OsTFIIB5 alters rice development and seed quality.","authors":"Shivam Sharma, Ankita Prusty, Prasant Kumar Dansana, Sanjay Kapoor, Akhilesh Kumar Tyagi","doi":"10.1007/s00299-025-03423-y","DOIUrl":"10.1007/s00299-025-03423-y","url":null,"abstract":"<p><strong>Key message: </strong>Overexpression of general transcription factor OsTFIIB5 in rice affects seedling growth, plant height, flowering time, panicle architecture, and seed protein/starch levels and involves modulation of expression of associated genes. TFIIB, a key general transcription factor (GTF), plays a critical role in pre-initiation complex (PIC) formation and facilitates RNA polymerase II-mediated transcription. In humans and yeast, TFIIB is encoded by a single gene; however, in plants it is encoded by a multigene family whose products may perform specialized transcriptional functions. The role of plant TFIIBs, particularly in monocots, remains largely unexplored. This study presents the first functional characterization of the rice TFIIB gene, OsTFIIB5 (LOC_Os09g36440), during development. Expression profiling of OsTFIIB5 revealed differential patterns across various developmental stages, with pronounced transcript accumulation during seed development. Overexpression of OsTFIIB5 impacted multiple stages of plant growth and development, leading to phenotypic changes such as altered seedling growth, reduced plant height, early heading, altered panicle architecture, decreased yield, and changes in seed storage substances. Notably, there were no effects on seed germination, pollen development, and grain size. Reduction in shoot length and plant height was linked to altered expression of genes involved in gibberellin (GA) biosynthesis, signalling, and deactivation. Overexpression of OsTFIIB5 enhanced the expression of genes involved in the photoperiodic flowering pathway, resulting in early panicle emergence. Higher expression levels of OsTFIIB5 also induced the accumulation of seed storage proteins (SSPs), while reducing starch content and altering the proportions of amylose and amylopectin in seeds. These findings suggest that OsTFIIB5 functions as a transcriptional regulator, governing multiple aspects of rice growth and development.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"44 2","pages":"27"},"PeriodicalIF":5.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142966338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}