Plant Cell Reports最新文献

{"title":"Genome-wide association study identified BnaPAP17 genes involved in exogenous ATP utilization and regulating phosphorous content in Brassica napus.","authors":"Hao Li, Haijiang Liu, Chuang Wang, Yang Zeng, Surya Kant, Xiaohua Wang, John P Hammond, Guangda Ding, Hongmei Cai, Sheliang Wang, Fangsen Xu, Ying Zhang, Lei Shi","doi":"10.1007/s00299-024-03373-x","DOIUrl":"10.1007/s00299-024-03373-x","url":null,"abstract":"<p><strong>Key message: </strong>BnaPAP17s associated with root-secreted APases activity were identified by genome-wide association study, and those were induced by Pi-deficiency. BnaPAP17s were involved in improving exogenous organophosphorus utilization as secreted APases. Deficiency of available phosphorus (P) in soil has become an important limiting factor for yield and quality in oilseed rape (Brassica napus). In many soils, organic P (Po) is the main component of the soil P pool. Po must be hydrolyzed to inorganic P (Pi) through acid Phosphatase (APases), and then taken up by plants. However, root-secreted APases (SAP) activity, as a quantitative trait, plays an important role in soil Po utilization; those genetic loci are not clear in B. napus. In this study, we performed a genome-wide association study for SAP activity under Pi-deficiency using a panel of 350 accessions of B. napus and more than 4.5 million polymorphic single nucleotide polymorphisms (SNPs). Thirty-five significant SNPs associated with SAP activity were identified. BnaA01.PAP17 (BnaA01g27810D) was a candidate gene underlying lead SNP (ChrA01_19576615). We experimentally verified that both BnaA01.PAP17 and its three homologous genes had similar expression pattern in response to Pi-deficiency. The dynamic changes in BnaPAP17s expression level were opposite to those of Pi concentration in both roots and leaves, suggesting their potential utility as Pi marker genes in B. napus. Transient expression of BnaPAP17s in tobacco leaves proved that BnaPAP17s were located in the apoplast as secreted APases. The overexpression of BnaPAP17s enhanced SAP activity in response to Pi-deficiency and resulting in increased P content in plants when ATP was supplied as the sole P resource. Taken together, these results suggest that BnaPAP17s contributed to SAP activity, thus having a function in extracellular Po utilization in B. napus.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"296"},"PeriodicalIF":5.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two genes encoding a bacterial-type ABC transporter function in aluminum tolerance in soybean.","authors":"Junjun Huang, Huanan Li, Yiwei Chen, Xiaoyu Li, Ziyu Jia, Kunxia Cheng, Luyu Wang, Huahua Wang","doi":"10.1007/s00299-024-03381-x","DOIUrl":"10.1007/s00299-024-03381-x","url":null,"abstract":"<p><strong>Key message: </strong>GmABCI5 and GmABCI13 enhance Al tolerance through regulating the composition of root cell wall, and in this process, GmABCI5 and GmABCI13 may act in the form of a complex. Aluminum (Al) toxicity is a major factor limiting plant growth in acidic soils. ATP-binding cassette (ABC) transporters are involved in plant tolerance to various environmental stresses. However, there are few reports on the ABC transporters implicated in soybean tolerance to Al toxicity. Here, we reported that two genes, GmABCI5 and GmABCI13, were involved in Al tolerance in soybean (Glycine max). GmABCI5 and GmABCI13 encode a nucleotide-binding domain and a transmembrane domain of a bacterial-type ABC transporter, respectively. The expression of both GmABCI5 and GmABCI13 was mainly induced by Al in the roots. GmABCI5 was localized at the plasma membrane and also in the cytoplasm and nucleus, while GmABCI13 was only localized at the plasma membrane. Furthermore, GmABCI5 could physically interact with GmABCI13. Overexpression of GmABCI5 or GmABCI13 in Arabidopsis reduced Al accumulation in roots and enhanced Al tolerance. However, expression of GmABCI5 and/or GmABCI13 in yeast cells did not affect Al uptake. Under Al stress, transgenic Arabidopsis lines expressing GmABCI5 or GmABCI13 had lower Al content in root cell walls than wild-type plants. Further analysis showed that Al content in cell wall fractions (pectin and hemicellulose 1) of transgenic lines was significantly lower than that of wild-type plants, which was coincident with the changes of pectin and hemicellulose 1 content under Al stress. These results indicate that GmABCI5 and GmABCI13 form an ABC transporter complex to regulate Al tolerance by affecting the modification of cell wall.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"295"},"PeriodicalIF":5.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell transcriptomics: a new frontier in plant biotechnology research.","authors":"Shilpy Singh, Afsana Praveen, Namrata Dudha, Varun Kumar Sharma, Pooja Bhadrecha","doi":"10.1007/s00299-024-03383-9","DOIUrl":"10.1007/s00299-024-03383-9","url":null,"abstract":"<p><p>Single-cell transcriptomic techniques have ushered in a new era in plant biology, enabling detailed analysis of gene expression at the resolution of individual cells. This review delves into the transformative impact of these technologies on our understanding of plant development and their far-reaching implications for plant biotechnology. We present a comprehensive overview of the latest advancements in single-cell transcriptomics, emphasizing their application in elucidating complex cellular processes and developmental pathways in plants. By dissecting the heterogeneity of cell populations, single-cell technologies offer unparalleled insights into the intricate regulatory networks governing plant growth, differentiation, and response to environmental stimuli. This review covers the spectrum of single-cell approaches, from pioneering techniques such as single-cell RNA sequencing (scRNA-seq) to emerging methodologies that enhance resolution and accuracy. In addition to showcasing the technological innovations, we address the challenges and limitations associated with single-cell transcriptomics in plants. These include issues related to sample preparation, cell isolation, data complexity, and computational analysis. We propose strategies to mitigate these challenges, such as optimizing protocols for protoplast isolation, improving computational tools for data integration, and developing robust pipelines for data interpretation. Furthermore, we explore the practical applications of single-cell transcriptomics in plant biotechnology. These applications span from improving crop traits through precise genetic modifications to enhancing our understanding of plant-microbe interactions. The review also touches on the potential for single-cell approaches to accelerate breeding programs and contribute to sustainable agriculture. This review concludes with a forward-looking perspective on the future impact of single-cell technologies in plant research. We foresee these tools becoming essential in plant biotechnology, spurring innovations that tackle global challenges in food security and environmental sustainability. This review serves as a valuable resource for researchers, providing a roadmap from sample preparation to data analysis and highlighting the transformative potential of single-cell transcriptomics in plant biotechnology.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"294"},"PeriodicalIF":5.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Touch, light, wounding: how anaesthetics affect plant sensing abilities.","authors":"Andrej Pavlovič","doi":"10.1007/s00299-024-03369-7","DOIUrl":"10.1007/s00299-024-03369-7","url":null,"abstract":"<p><strong>Key message: </strong>Anaesthetics affect not only humans and animals but also plants. Plants exposed to certain anaesthetics lose their ability to respond adequately to various stimuli such as touch, injury or light. Available results indicate that anaesthetics modulate ion channel activities in plants, e.g. Ca²⁺ influx. The word anaesthesia means loss of sensation. Plants, as all living creatures, can also sense their environment and they are susceptible to anaesthesia. Although some anaesthetics are often known as drugs with well-defined target to their animal/human receptors, some other are promiscuous in their binding. Both have effects on plants. Application of general volatile anaesthetics (GVAs) inhibits plant responses to different stimuli but also induces strong cellular response. Of particular interest is the ability of GVAs inhibit long-distance electrical and Ca²⁺ signalling probably through inhibition of GLUTAMATE RECEPTOR-LIKE proteins (GLRs), the effect which is surprisingly very similar to inhibition of nerve impulse transmission in animals or human. However, GVAs act also as a stressor for plants and can induce their own Ca²⁺ signature, which strongly reprograms gene expression . Down-regulation of genes encoding enzymes of chlorophyll biosynthesis and pigment-protein complexes are responsible for inhibited de-etiolation and photomorphogenesis. Vesicle trafficking, germination, and circumnutation movement of climbing plants are also strongly inhibited. On the other hand, other cellular processes can be upregulated, for example, heat shock response and generation of reactive oxygen species (ROS). Upregulation of stress response by GVAs results in preconditioning/priming and can be helpful to withstand abiotic stresses in plants. Thus, anaesthetic drugs may become a useful tool for scientists studying plant responses to environmental stimuli.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"293"},"PeriodicalIF":5.3,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A weak allele of AtECB2, a member of the pentatricopeptide repeat motif superfamily, causes leaf virescence in Arabidopsis.","authors":"Ya-Ping Liang, Xue-Wen Hou","doi":"10.1007/s00299-024-03376-8","DOIUrl":"10.1007/s00299-024-03376-8","url":null,"abstract":"<p><strong>Key message: </strong>Through the study of a point mutation of AtECB2, it is reconfirmed that AtECB2 plays an important role in the early development of chloroplast. AtECB2(EARLY CHLOROPLAST BIOGENESIS 2, At1g15510), a member of the pentatricopeptide repeat motif proteins (PPR) superfamily, and its loss of function mutation ecb2-1causes seedling lethal, while a point mutation ecb2-2 causes delayed chloroplast development. Finding more AtECB2 weak alleles helps to understand the molecular mechanisms of AtECB2. In this study, a leaf virescence mutant was identified from ethylmethane sulfonate (EMS) treated Arabidopsis Col-0 M2 mutants library. The mutation of this mutant was first confirmed as a recessive mutation of one gene through the phenotype of F1 and its F2 phenotype segregation of this mutant crossed with Col-0. The mutation of G1931A of AtECB2 is identified as the cause of this leaf virescence phenotype sequentially through positional cloning, whole genome resequencing, Sanger sequencing and complementation. Therefore, we named this weak allele of AtECB2 as ecb2-3. The chlorophyll content and photosystem II maximum photochemical efficiency of ecb2-3 are obviously lower than that of Col-0 and its complementation lines, respectively. The chloroplast development of ecb2-3 is also inferior to that of Col-0 and its complementation line at the observed time points using the transmission electron microscope. The RNA editing efficiency of three chloroplast gene sites (accD C794 and C1568, ndhF C290) was observed much lower compared with that of Col-0 and its complementation line. In summary, AtECB2 plays an important role in early chloroplast biogenesis through related chloroplast gene editing regulation, and this weak mutant ecb2-3 may be useful material in dissecting the function of AtECB2 in the near future.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"292"},"PeriodicalIF":5.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation of bacterial natural single guide RNA (tracr-L) for efficient plant genome editing.","authors":"Subhasis Karmakar, Debasmita Panda, Deeptirekha Behera, Romio Saha, Mirza J Baig, Kutubuddin Ali Molla","doi":"10.1007/s00299-024-03371-z","DOIUrl":"10.1007/s00299-024-03371-z","url":null,"abstract":"<p><strong>Key message: </strong>A long tracrRNA (tracr-L), which naturally act as single guide RNA, and its truncated version, Δtracr-L, from S. pyogenes, efficiently induce Cas9-mediated double-strand breaks (DSBs) in plant genomic loci, as demonstrated by in vitro cleavage assay and protoplast transfection. CRISPR-Cas system provides a form of immune memory in prokaryotes and archaea, protecting them against viruses and foreign genetic elements. In Streptococcus pyogenes, this system includes the pre-crRNA along with another non-coding RNA, tracrRNA, which aids in CRISPR-based immunity. In S. pyogenes, two distinct tracrRNAs are produced: a long form (tracr-L) and a short form (tracr-S). The tracr-S regulates crRNA biogenesis and Cas9 cleavage, while tracr-L suppresses CRISPR-Cas expression by targeting the Cas9 promoter to prevent autoimmunity. Deleting 79 nucleotides from tracr-L results in Δtracr-L, which retains similar functionality in gene repression. This study investigates, for the first time, the effectiveness of tracr-L, and Δtracr-L in genome editing within plant systems. In vitro cleavage assays using purified Cas9 and synthesized sgRNAs targeting the Cas9 gene, OsPDS, and the OsSWEET11 promoter revealed that across all target sites, tracr-S demonstrated the highest cleavage efficiency compared to tracr-L and Δtracr-L. For in vivo genome editing, we transfected rice protoplasts with tracr-L, Δtracr-L, and tracr-S, targeting three rice genes: OsPDS, OsSPL14, and the promoter of OsSWEET14. Amplicon deep sequencing revealed various types of indels at the target regions across all three tracrRNA versions, indicating comparable levels of efficiency. This study establishes the utility of both the long-form tracrRNA (tracr-L) and its truncated variant (Δtracr-L) in eukaryote genome editing. These two new forms of tracrRNA provide proof of concept and expand the CRISPR-Cas toolkit for plant genome editing applications, and for eukaryotes more broadly.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"291"},"PeriodicalIF":5.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of hub genes regulating nitrate and glucose response of nitrate response deficiency 1 (NRD1) mutant in foxtail millet.","authors":"Xin-Li Yao, Zi-Dong Li, Ming-Hua Zhang, Hui-Xin Meng, Yu-Ze Wang, Shuqi Dong, Xiangyang Yuan, Xiaorui Li, Lulu Gao, Guanghui Yang, Xiaoqian Chu, Jia-Gang Wang","doi":"10.1007/s00299-024-03379-5","DOIUrl":"10.1007/s00299-024-03379-5","url":null,"abstract":"","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"289"},"PeriodicalIF":5.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation marker-free transplastomic plants: engineering the chloroplast genome without integration of marker genes in Solanum tuberosum (potato).","authors":"Alessandro Occhialini, Andrew C Reed, Stacee A Harbison, Megan J Sichterman, Aaron Baumann, Alexander C Pfotenhauer, Li Li, Gabriella King, Aaron G Vincent, Ashley D Wise-Mitchell, C Neal Stewart, Scott C Lenaghan","doi":"10.1007/s00299-024-03375-9","DOIUrl":"10.1007/s00299-024-03375-9","url":null,"abstract":"<p><strong>Key message: </strong>This study describes an optimized plastid genetic engineering platform to produce full marker-free transplastomic plants with transgene integrated at homoplasmy in one step in tissue culture. Plastid engineering is attractive for both biotechnology and crop improvement due to natural bio-confinement from maternal inheritance, the absence of transgene positional effects and silencing, the ability to express transgenes in operons, and unparalleled production of heterologous proteins. While plastid engineering has had numerous successes in the production of high-value compounds, no transplastomic plants have been approved for use in agriculture. In order for transplastomic plants to be used in agriculture, the removal of antibiotic selection genes is required. In this work, we developed an optimized strategy to generate homoplasmic marker-free lines of potato (Solanum tuberosum) in a single transformation event. To achieve marker-free transplastomic lines, vectors were redesigned to enable integration of the transgene cassette into the plastid genome, while maintaining the selection cassette on the vector backbone. After an initial round of tissue culture with selection, the selective pressure was removed, leading to the elimination of the vector backbone, while retaining the integrated transgene cassette at homoplasmy. Marker-free transplastomic lines produced using this strategy had a normal phenotype, and transgene integration was stable across generations. The new vectors developed in this work for the generation of marker-free transplastomics will represent a valuable alternative platform for routine plastid genetic engineering in higher plants. It is also anticipated that this approach will contribute to speed the path to commercialization of these novel transplastomic plant varieties.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"290"},"PeriodicalIF":5.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constitutive expression of cucumber CsACS2 in Arabidopsis Thaliana disrupts anther dehiscence through ethylene signaling and DNA methylation pathways.","authors":"Zonghui Yang, Libin Li, Zhaojuan Meng, Mingqi Wang, Tian Gao, Jingjuan Li, Lixia Zhu, Qiwei Cao","doi":"10.1007/s00299-024-03374-w","DOIUrl":"10.1007/s00299-024-03374-w","url":null,"abstract":"<p><strong>Key message: </strong>Constitutive expression of cucumber CsACS2 in Arabidopsis disrupts anther dehiscence and male fertility via ethylene signaling and DNA methylation, revealing new avenues for enhancing crop reproductive traits. The cucumber gene CsACS2, encoding ACC (1-aminocyclopropane-1-carboxylic acid) synthase, plays a pivotal role in ethylene biosynthesis and sex determination. This study investigates the effects of constitutive CsACS2 expression in Arabidopsis thaliana on anther development and male fertility. Transgenic Arabidopsis plants overexpressing CsACS2 exhibited male sterility due to inhibited anther dehiscence, which was linked to suppressed secondary cell wall thickening. RNA-Seq analysis revealed upregulation of ethylene signaling pathway genes and downregulation of secondary cell wall biosynthesis genes, with gene set enrichment analysis indicating the involvement of DNA methylation. Rescue experiments demonstrated that silver nitrate (AgNO₃) effectively restored fertility, while 5-azacytidine (5-az) partially restored it, highlighting the roles of ethylene signaling and DNA methylation in this process. Constitutive CsACS2 expression in Arabidopsis disrupts anther development through ethylene signaling and DNA methylation pathways, providing new insights into the role of ethylene in plant reproductive development and potential applications in crop improvement.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"288"},"PeriodicalIF":5.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of ABA function in endosperm cellularization and storage product accumulation.","authors":"Qing Liu, Xin Ye, Zhiwen Zhao, Qian Li, Cunxu Wei, Juan Wang","doi":"10.1007/s00299-024-03378-6","DOIUrl":"10.1007/s00299-024-03378-6","url":null,"abstract":"<p><p>Seed development is a complex process and co-regulated by genetic and environmental factors, which significantly affects the seed vigor, yield and quality of crops, especially in cereal crops. Abscisic acid (ABA) regulates various biological processes in seed development, including endosperm and embryo development, accumulation of storage materials, achievement of desiccation tolerance and dormancy. Compared to the functional investigation of ABA in germination and stress response, the role of ABA in early seed development and storage product accumulation has not been collectively elucidated. Here, ABA origin in seed was concluded: both maternal source and de novo synthesis of ABA in seed play an important role in seed development. This review also provided an overview of the current knowledge on ABA in early seed development, mainly in endosperm cellularization. ABA promotes endosperm cellularization in Arabidopsis, but this notion has not been spread into cereal crops. Besides, the increasing importance of ABA in seed reserve accumulation was also emphatically described. In the last section, the key problems and challenges (e.g., where dose ABA come from at each stage of seed development? whether same regulators in response to ABA in Arabidopsis apply equally to cereal crops) were addressed.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":"43 12","pages":"287"},"PeriodicalIF":5.3,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}