Transcriptomics-proteomics analysis reveals StCOMT1 regulates drought, alkali and combined stresses in potato.

IF 5.3 2区生物学 Q1 PLANT SCIENCES

Plant Cell Reports Pub Date : 2025-04-29 DOI:10.1007/s00299-025-03496-9

Ruyan Zhang, Yong Wang, Yichen Kang, Yunyun Du, Xingxing Wang, Shujuan Jiao, Xinyu Yang, Yuhui Liu, Shuhao Qin, Weina Zhang

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引用次数: 0

Abstract

Key message: Transcriptome proteome association analysis screened candidate DEGs, DEPs, and DEGs/DEPs associated with potato response to drought, alkali, and combined stresses. Overexpression of StCOMT1 enhances potato drought and alkali tolerance. Drought and salinity have severely impeded potato (Solanum tuberosum L.) growth and development, significantly reducing global potato production. However, the molecular mechanisms regulating the combined drought and alkali stress process are not fully understood. This study compared the mRNA and protein expression profiles of potato under drought (PEG-6000), alkali (NaHCO₃), and combined (PEG-6000 + NaHCO₃) stresses by transcriptome and TMT proteomics sequencing to investigate the common or specific responses of 'Atlantic' potato to single and combined stresses of drought and alkali were preliminarily explored. It was found that 2215 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were jointly identified under drought, alkali, and combined stresses. Under drought, alkali, and combined stresses, 234, 185, and 246 DEGs/DEPs were identified, respectively. These DEGs, DEPs, and DEGs/DEPs identified revealed the potential roles of several signaling and metabolic pathways in mediating drought and alkali stress tolerance, including plant hormone signaling, MAPK signaling pathway, phenylpropanoid biosynthesis, and glutathione metabolism. Caffeic acid-O-methyltransferase (COMT) is an essential methylating enzyme in the phenylpropane biosynthetic pathway, which is involved in lignin synthesis and plays an important role in protecting plants from abiotic stresses. In this study, we investigated the changes in physiologic characteristics, such as growth, antioxidant defense, osmotic regulation and lignin accumulation, in overexpressing StCOMT1 (PT0001512/M0ZIL7) transgenic potato after stress. It proved that the gene has the function of adapting to drought and alkali stress, and provided a theoretical basis for further research on the resistance mechanism of the gene in drought and alkali tolerance in potato.

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转录组学-蛋白质组学分析显示StCOMT1调控马铃薯干旱、碱和复合胁迫。

转录组蛋白组关联分析筛选了候选DEPs、DEPs和与马铃薯对干旱、碱和综合胁迫反应相关的DEPs /DEPs。StCOMT1过表达增强了马铃薯的耐旱性和耐碱性。干旱和盐碱化严重阻碍了马铃薯（Solanum tuberosum L.）的生长发育，导致全球马铃薯产量大幅下降。然而，调控旱碱联合胁迫过程的分子机制尚不完全清楚。本研究通过转录组和TMT蛋白质组学测序，比较了干旱（PEG-6000）、碱（NaHCO3）和复合（PEG-6000 + NaHCO3）胁迫下马铃薯的mRNA和蛋白表达谱，初步探讨了“大西洋”马铃薯对干旱和碱单一胁迫和复合胁迫的共同或特异性响应。结果发现，在干旱、碱和复合胁迫下，共鉴定出2215个差异表达基因（deg）和450个差异表达蛋白（dep）。在干旱、强碱和复合胁迫下，分别鉴定出234、185和246个deg / dep。这些DEGs、DEPs和DEGs/DEPs揭示了植物激素信号通路、MAPK信号通路、苯丙素生物合成和谷胱甘肽代谢等多种信号和代谢途径在介导干旱和碱胁迫中的潜在作用。咖啡酸o -甲基转移酶（coffic acid-O-methyltransferase， COMT）是苯丙烷生物合成途径中必需的甲基化酶，参与木质素的合成，在保护植物免受非生物胁迫中发挥重要作用。本研究研究了StCOMT1 （PT0001512/M0ZIL7）转基因马铃薯过表达胁迫后生长、抗氧化防御、渗透调节和木质素积累等生理特性的变化。证明该基因具有适应干旱和碱胁迫的功能，为进一步研究该基因在马铃薯抗旱耐碱中的抗性机制提供了理论依据。

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来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.