A novel-miR-164 from Atractylodes lancea targets AlNAC1 regulating leaf senescence.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Juan Deng, Xiao Huang, Meng Wang, Qian Li, Ling Gong, Bisheng Huang, Kun Yu
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引用次数: 0

Abstract

Key message: A novel miR164-AlNAC1 module adjusting the expression of genes related to chlorophyll synthesis and ROS synthesis, and regulating leaf senescence in Atractylodes lancea. Leaf senescence is a critical developmental process regulated by complex genetic networks. In this study, a novel miRNA belonging to the MIR164 family was identified as a key regulator of leaf senescence in A. lancea by targeting the transcription factor AlNAC1. Bioinformatics analysis revealed that target gene AlNAC1 was closely related to the senescence-associated gene ANAC092 in Arabidopsis. The qRT-PCR and tobacco transient expression demonstrated nov-miR164 negatively regulated AlNAC1. Subcellular localization and transcriptional activity assays revealed that AlNAC1 localized to the nucleus and functioned as a transcriptional activator. Furthermore, EMSA experiments showed that AlNAC1 bound to the G-box motifs on the promoters of AlNYC, AlPAO, and AlRbohC. With the aging of A. lancea leaves, the expression of nov-miR164 decreased, while AlNAC1 and senescence-associated genes (AlSAG21, AlNYC, AlPAO, and AlRbohC) were significantly upregulated. Agrobacterium-mediated transient expression experiment results demonstrated that nov-miR164 negatively regulated AlNAC1 to suppress chlorophyll degradation and ROS accumulation, thereby delaying leaf senescence. Our findings provide new insights into the molecular mechanisms of leaf senescence in A. lancea and highlight the potential of nov-miR164 as a target for increasing crop yield and improving crop longevity.

来自苍术的新型mir -164靶向调节叶片衰老的AlNAC1。
关键信息:一个新的miR164-AlNAC1模块调节白术叶绿素合成和ROS合成相关基因的表达,并调节白术叶片衰老。叶片衰老是一个重要的发育过程,受复杂的遗传网络调控。在本研究中,通过靶向转录因子AlNAC1,发现了一个属于MIR164家族的新miRNA是杉木叶片衰老的关键调控因子。生物信息学分析表明,靶基因AlNAC1与拟南芥衰老相关基因ANAC092密切相关。qRT-PCR和烟草瞬时表达证实,novo - mir164负调控AlNAC1。亚细胞定位和转录活性分析表明,AlNAC1定位于细胞核,并作为转录激活因子发挥作用。此外,EMSA实验表明,AlNAC1与AlNYC、AlPAO和AlRbohC启动子上的G-box基序结合。随着杉木叶片的衰老,novo - mir164的表达量下降,而AlNAC1和衰老相关基因(AlSAG21、AlNYC、AlPAO和AlRbohC)的表达量显著上调。农杆菌介导的瞬时表达实验结果表明,novo - mir164负调控AlNAC1,抑制叶绿素降解和ROS积累,从而延缓叶片衰老。我们的研究结果为刺楠叶片衰老的分子机制提供了新的见解,并突出了novo - mir164作为提高作物产量和延长作物寿命的靶点的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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