Xiaomin Zhao, Li Chen, Yuan Huang, Junjie Hu, Jing Zhang, Bin Zhang, Zhiqing Ma
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引用次数: 0
Abstract
Key message: The overexpression of key biosynthetic genes involved in triptolide production through a metabolic engineering strategy significantly enhanced triptolide accumulation in Tripterygium wilfordii hairy roots. Triptolide, the representative bioactive compound in Tripterygium wilfordii, is renowned for its potent insecticidal and pharmacological properties. In order to increase the production of triptolide, this study overexpressed several key enzyme genes related to its biosynthesis in T. wilfordii hairy roots. Specifically, the content of triptolide in hairy roots overexpressing TwTPS9 and TwTPS27 individually was found to be 1.60-fold and 1.42-fold that of the control, respectively. Co-expression of both TwTPS9 and TwTPS27 resulted in significant increase in triptolide levels, reaching approximately 2.72 times that of the control. Furthermore, overexpressing TwGGPPS and TwDXS on the basis of the double gene overexpression led to the highest triptolide production, with a yield of 12.83 mg/L, increasing to 3.18-fold compared to the control. This study offers valuable examples into the efficient biosynthesis of triptolide and is expected to lay a foundation for future industrial-scale production by mitigating its resource constraints through metabolic engineering.
期刊介绍:
Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as:
- genomics and genetics
- metabolism
- cell biology
- abiotic and biotic stress
- phytopathology
- gene transfer and expression
- molecular pharming
- systems biology
- nanobiotechnology
- genome editing
- phenomics and synthetic biology
The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.