Targeted metabolic engineering of key biosynthetic genes improves triptolide production in Tripterygium wilfordii hairy roots.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Xiaomin Zhao, Li Chen, Yuan Huang, Junjie Hu, Jing Zhang, Bin Zhang, Zhiqing Ma
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引用次数: 0

Abstract

Key message: The overexpression of key biosynthetic genes involved in triptolide production through a metabolic engineering strategy significantly enhanced triptolide accumulation in Tripterygium wilfordii hairy roots. Triptolide, the representative bioactive compound in Tripterygium wilfordii, is renowned for its potent insecticidal and pharmacological properties. In order to increase the production of triptolide, this study overexpressed several key enzyme genes related to its biosynthesis in T. wilfordii hairy roots. Specifically, the content of triptolide in hairy roots overexpressing TwTPS9 and TwTPS27 individually was found to be 1.60-fold and 1.42-fold that of the control, respectively. Co-expression of both TwTPS9 and TwTPS27 resulted in significant increase in triptolide levels, reaching approximately 2.72 times that of the control. Furthermore, overexpressing TwGGPPS and TwDXS on the basis of the double gene overexpression led to the highest triptolide production, with a yield of 12.83 mg/L, increasing to 3.18-fold compared to the control. This study offers valuable examples into the efficient biosynthesis of triptolide and is expected to lay a foundation for future industrial-scale production by mitigating its resource constraints through metabolic engineering.

关键生物合成基因的靶向代谢工程提高雷公藤毛状根雷公藤甲素的产量。
关键信息:通过代谢工程策略过度表达参与雷公藤甲素生产的关键生物合成基因,显著增强了雷公藤毛状根中雷公藤甲素的积累。雷公藤甲素是雷公藤中具有代表性的生物活性化合物,以其有效的杀虫和药理特性而闻名。为了提高雷公藤甲素的产量,本研究在雷公藤毛状根中过表达了与雷公藤甲素生物合成相关的几个关键酶基因。其中,分别过表达TwTPS9和TwTPS27的毛状根中雷公藤甲素含量分别是对照的1.60倍和1.42倍。TwTPS9和TwTPS27的共表达导致雷公藤甲素水平显著升高,约为对照组的2.72倍。此外,在双基因过表达的基础上,过表达TwGGPPS和TwDXS的雷公藤甲素产量最高,为12.83 mg/L,比对照提高了3.18倍。该研究为雷公藤甲素的高效生物合成提供了有价值的范例,并有望通过代谢工程减轻其资源限制,为未来的工业规模生产奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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