Plant Cell最新文献_第8页

Vascular-specific genome editing enhances low-phosphate tolerance in rice. 维管特异性基因组编辑增强水稻低磷耐受性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag203

Ju-Chen Chia

引用次数: 0

Ubiquitination of the ribosomal-like domain of UBIQUITIN6 contributes to cell wall stress responses in Arabidopsis. UBIQUITIN6核糖体样结构域的泛素化参与拟南芥细胞壁应激反应。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag187

Yu Zhu, Eduardo Antonio Ramírez Rodríguez, Fabrizio Chow, Fayeza Tabassum Azad, Una McNally, Hee Sung Kang, Berit Ebert, Heather E McFarlane

{"title":"Ubiquitination of the ribosomal-like domain of UBIQUITIN6 contributes to cell wall stress responses in Arabidopsis.","authors":"Yu Zhu, Eduardo Antonio Ramírez Rodríguez, Fabrizio Chow, Fayeza Tabassum Azad, Una McNally, Hee Sung Kang, Berit Ebert, Heather E McFarlane","doi":"10.1093/plcell/koag187","DOIUrl":"10.1093/plcell/koag187","url":null,"abstract":"Plants must constantly sense the status of their cell walls and respond by modifying cell walls to maintain growth and respond to developmental and external stimuli. This capacity to sense cell walls and mount appropriate responses are collectively called cell wall signaling. Reversible post-translational modifications, such as phosphorylation or ubiquitination, are often an important part of signal transduction since they can rapidly and reversibly modify protein function and/or localization. To uncover differential post-translational modifications during cell wall stress, we analyzed the ubiquitinated proteome of Arabidopsis seedlings treated with the cell wall synthesis inhibitor, isoxaben. We identified 49 proteins that are significantly differentially ubiquitinated under short-term isoxaben treatment. We examined the phenotypes of loss-of-function mutants affecting one of these candidates, UBQ6, and found that ubq6 mutants are relatively resistant to isoxaben. This resistance is partially conveyed by sustained cellulose synthesis during isoxaben treatment. We further found that UBQ6 is a ubiquitin extension protein family member and that it is cleaved into two domains: the ubiquitin-like domain and the ribosomal-like domain. We confirmed that the site that is differentially ubiquitinated under cell wall stress is within the ribosomal-like domain of UBQ6 and found that the ribosomal-like domain is required for wild-type cell wall stress responses. We propose a model in which ubiquitination of the ribosomal-like domain of a UBQ6 has a specific role in cell wall signaling and cell wall stress responses, independent of the ubiquitin molecule created by UBQ6 protein cleavage.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13411792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allelic variation in LcARF10 toggles an auxin-responsive MYB70-ARF10 repressor switch governing litchi fruit acidity. LcARF10的等位基因变异会改变生长素响应MYB70-ARF10抑制因子开关，控制荔枝果实的酸度。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag200

Bo Zhang, Jian Zheng, Jinchang Yu, Huiyun Zhang, Jiaxin Fu, Jietang Zhao, Zhike Zhang, Yonghua Qin, Xinping Luo, Xiaofeng Huang, Huicong Wang, Guibing Hu

{"title":"Allelic variation in LcARF10 toggles an auxin-responsive MYB70-ARF10 repressor switch governing litchi fruit acidity.","authors":"Bo Zhang, Jian Zheng, Jinchang Yu, Huiyun Zhang, Jiaxin Fu, Jietang Zhao, Zhike Zhang, Yonghua Qin, Xinping Luo, Xiaofeng Huang, Huicong Wang, Guibing Hu","doi":"10.1093/plcell/koag200","DOIUrl":"10.1093/plcell/koag200","url":null,"abstract":"Fruit acidity, a key quality trait, is primarily determined by malic acid in many fruit crops. However, the molecular machinery governing its accumulation, particularly in response to hormonal cues like auxin, remains a \"black box.\" Here, we systematically deconstruct the regulatory pathway controlling malic acid in litchi (Litchi chinensis). We first identify the tonoplast-localized transporter LcALMT1 as the principal channel for malate sequestration into the vacuole. We then uncover its upstream regulatory module, revealing that the R2R3-MYB transcription factor LcMYB70 directly binds the LcALMT1 promoter but lacks intrinsic transcriptional activity. Instead, LcMYB70 functions as a molecular scaffold to recruit the Auxin Response Factor LcARF10, which confers transcriptional repression upon the complex. This LcMYB70-LcARF10 repressor module is negatively regulated by auxin signaling, providing a direct molecular link between the decline in endogenous auxin during fruit maturation and the attenuation of malate accumulation. Critically, we identified a single-nucleotide polymorphism in the LcARF10 gene that governs the divergence between high- and low-acid cultivars. The functional C allele in low-acid cultivars enables the formation of the repressor complex. In contrast, the T allele in high-acid cultivars contains a premature stop codon that results in a non-functional, truncated protein, preventing complex assembly and causing the high-acid phenotype. Our study defines a complete pathway from hormone to trait and its genetic basis, revealing a novel MYB-ARF scaffold module; a definitive molecular link between auxin withdrawal and metabolic reprogramming; and the functional integrity of a corepressor as a new paradigm for trait diversification.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 7","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148606420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses. STA1-DOT2相互作用促进生长和热应激反应中的核斑形成和剪接稳健性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag207

Heejin Kim, Kyoung-Jae Yu, So Young Park, Dong Hye Seo, Woo Taek Kim, Dae-Jin Yun, Jae-Hoon Jung, Dong-Hoon Jeong, Byeong-Ha Lee

{"title":"The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses.","authors":"Heejin Kim, Kyoung-Jae Yu, So Young Park, Dong Hye Seo, Woo Taek Kim, Dae-Jin Yun, Jae-Hoon Jung, Dong-Hoon Jeong, Byeong-Ha Lee","doi":"10.1093/plcell/koag207","DOIUrl":"10.1093/plcell/koag207","url":null,"abstract":"Pre-mRNA splicing is carried out by the spliceosome, a large and dynamic ribonucleoprotein complex. The spliceosome is known to be stored in nuclear speckles (NS), which are recognized as active subnuclear organelles for splicing. However, it remains poorly understood how spliceosomal protein-protein interactions are functionally coupled to NS organization to maintain splicing robustness in plants. Here, we report the functional significance of a specific interaction between 2 U4/U6·U5 tri-snRNP components of the spliceosome, STA1 and DOT2, in regulating NS organization, pre-mRNA splicing, and heat stress responses in Arabidopsis. We identified a missense mutation in DOT2 (a Snu66/SART1 homolog) from a genetic suppressor of the PRP6 homolog mutant sta1-1 (named S307). This mutation restored the weakened interaction between STA1 and DOT2 in the sta1-1 mutant background. Genetic, biochemical, and cell biological analyses showed that variation in the strength of the STA1-DOT2 interaction was closely associated with changes in NS formation, splicing efficiency, transcript abundance, as well as growth and heat tolerance. Pharmacological inhibition of STA1-associated NS formation by tubercidin recapitulated sta1-1-like phenotypes and splicing defects, supporting a functional link between NS organization and splicing outcomes. In addition, heat-induced weakening of the STA1-DOT2 interaction was accompanied by reduced NS formation and increased intron retention at the transcriptome-wide level, including key heat-responsive transcripts. Based on these observations, we propose that the STA1-DOT2 interaction, likely reflecting the assembly state of the U4/U6·U5 tri-snRNP, may represent one of the heat-sensitive interaction nodes that couple spliceosome assembly to NS organization and splicing robustness under stress conditions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dehydration-induced condensation of AGO1 modulates miRNA functionality. 脱水诱导的ago2缩合可调节miRNA功能。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag155

Hyun Ju Jung, Tae Rin Oh, Woorim Yang, Jee-Eun Kim, Christian Møller, Moon Young Ryu, Bora Lee, Mi Young Byun, Hari Chandana Yadavalli, Suk Won Choi, Min Kyun Han, Young Kyoung Oh, Donghyuk Kim, Jin Young Shin, Tae-Hwan Kim, Jong Hum Kim, Taijoon Chung, Jae-Hoon Jung, Phil Hyu Lee, Woo Taek Kim, Seok Keun Cho, Seong Wook Yang

{"title":"Dehydration-induced condensation of AGO1 modulates miRNA functionality.","authors":"Hyun Ju Jung, Tae Rin Oh, Woorim Yang, Jee-Eun Kim, Christian Møller, Moon Young Ryu, Bora Lee, Mi Young Byun, Hari Chandana Yadavalli, Suk Won Choi, Min Kyun Han, Young Kyoung Oh, Donghyuk Kim, Jin Young Shin, Tae-Hwan Kim, Jong Hum Kim, Taijoon Chung, Jae-Hoon Jung, Phil Hyu Lee, Woo Taek Kim, Seok Keun Cho, Seong Wook Yang","doi":"10.1093/plcell/koag155","DOIUrl":"10.1093/plcell/koag155","url":null,"abstract":"Recent studies have shown that microRNA (miRNA) functions are associated with plant responses to water deficiency. Mature miRNAs are loaded onto a complex that includes the ARGONAUTE 1 (AGO1) protein; this complex then cleaves mRNAs or inhibits their translation. Here, we demonstrate that the prion-like domain of AGO1 is responsible for topological changes in AGO1 under dehydration in Arabidopsis thaliana. AGO1 undergoes liquid-liquid phase separation (LLPS), which is driven by intrinsically disordered protein domains and plays diverse roles in cellular processes. LLPS of AGO in the cytoplasm influences miRNA regulatory activity, a process related to cytoplasmic calcium levels. We found that dehydration-induced AGO1 condensation influences AGO1 activity, while contributing to dehydration tolerance in plants. Upon rehydration, the condensation-driven accumulation of AGO1 is resolved, restoring its protein levels to normal. Overall, we propose that AGO1 phase separation acts as an emergent property in response to dehydration, attenuating the energy-consuming miRNA regulatory pathway in young seedlings.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148056281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Landscape of plastid DNA breaks in Arabidopsis during development and environmental stimulus. 拟南芥发育过程中质体DNA断裂的景观和环境刺激。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag186

Wenjie Wang, Chengxia Zheng, Kuan Li, Qianwen Sun

{"title":"Landscape of plastid DNA breaks in Arabidopsis during development and environmental stimulus.","authors":"Wenjie Wang, Chengxia Zheng, Kuan Li, Qianwen Sun","doi":"10.1093/plcell/koag186","DOIUrl":"10.1093/plcell/koag186","url":null,"abstract":"External and internal environmental factors can lead to DNA breaks. DNA breaks in eukaryotic nuclear genomes have been extensively studied, but the landscape of DNA breaks in endosymbiotic organelles remains poorly understood. Here, we employed DNA end tailing and sequencing to profile DNA breaks in the Arabidopsis (Arabidopsis thaliana) plastid genome. We find that plastid DNA in cotyledons and true leaves remains relatively stable during the juvenile stage but undergoes extensive breaks in older tissues. Seeds had significantly lower break levels than leaves. Notably, ribosomal DNA regions experience more breaks, with a preference for the template strand. We further investigated plastid DNA break profiles in wild-type (Col-0) plants vs mutants defective in DNA repair, replication, and transcription under varying light, temperature, and photoperiod conditions. Wild-type plants maintained genome integrity under most tested conditions, but the plastid DNA damage repair and replication mutants why1/3/reca1 (lacking Whirly1 (WHY1), WHY3, and RECA1), why1/3/polIb (lacking WHY1/3 and DNA polymerase IB), and atrnh1c (lacking RNase H1C), did not. Additionally, the accumulation of R-loops and reactive oxygen species (ROS) potently induces plastid DNA breaks that are likely mediated by 8-oxoG modifications. Shortening the photoperiod alleviates plastid DNA breaks in Col-0, with an even more pronounced effect in atrnh1c. This study provides a genome-wide view of plastid DNA break dynamics and advances our understanding of damage and repair mechanisms in the organellar genome.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412044/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiomics studies reveal how ambient temperature changes govern cellular responses of Chlamydomonas. 多组学研究揭示了环境温度变化如何控制衣藻的细胞反应。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag136

Prateek Shetty, Trang Vuong, Chulin Li, Volker Wagner, Dinara Myrzakhmetova, Chia-Chi Peng, Wenshuang Li, Joel Ching, Ariane Zander, Sophie Weiser, Miriam A Rosenbaum, Rosalind J Allen, Markus Lakemeyer, Maria Mittag

{"title":"Multiomics studies reveal how ambient temperature changes govern cellular responses of Chlamydomonas.","authors":"Prateek Shetty, Trang Vuong, Chulin Li, Volker Wagner, Dinara Myrzakhmetova, Chia-Chi Peng, Wenshuang Li, Joel Ching, Ariane Zander, Sophie Weiser, Miriam A Rosenbaum, Rosalind J Allen, Markus Lakemeyer, Maria Mittag","doi":"10.1093/plcell/koag136","DOIUrl":"10.1093/plcell/koag136","url":null,"abstract":"Photosynthetic protists, known as microalgae, face increasing temperatures due to climate change. The green biflagellate alga Chlamydomonas reinhardtii (Chlamydomonas) serves as a model for thermoregulation. While responses to thermal stress are well characterized, much less is known about the impact of ambient temperature shifts. Understanding microalgal responses to environmental temperature changes is critical, as these primary producers drive ecosystem productivity and food web dynamics. Here, Chlamydomonas grew mixotrophically at ambient temperatures from 18 °C to 33 °C. Transcriptomic profiling revealed extensive reorganization, with over 5,000 transcripts significantly affected, including those involved in algal-bacterial interactions, photoreception, lipid metabolism, photosynthesis, cilia formation, and the secretome. CO2 transfer rates and acetate levels measured at 18 °C and 28 °C suggest decreased photoautotrophic algal growth at 28 °C at first. Antagonistic bacterial activity was sustained longer at lower temperatures. Proteomic analyses of isolated cilia and secreted proteins corroborate major abundance changes within these sub-proteomes, particularly in ciliary intraflagellar transport complexes and mating-related proteins in the secretome. Together, these molecular alterations resulted in pronounced changes in growth, the lengths of cells and cilia swimming behavior, mating ability, and bacterial antagonism. These data reveal major cellular responses caused by ambient, even short-term temperature shifts.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13457920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GhPsbO breaks the growth-immunity tradeoff by simultaneously promoting growth and defense in cotton. GhPsbO通过同时促进棉花的生长和防御，打破了生长与免疫之间的平衡。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag190

Jianing Li, Qiankun Wang, Hang Zhao, Ye Wang, Yanli Chen, Haodan Zhou, Hongying Duan, Lisen Liu, Fuguang Li, Xiaoyang Ge

{"title":"GhPsbO breaks the growth-immunity tradeoff by simultaneously promoting growth and defense in cotton.","authors":"Jianing Li, Qiankun Wang, Hang Zhao, Ye Wang, Yanli Chen, Haodan Zhou, Hongying Duan, Lisen Liu, Fuguang Li, Xiaoyang Ge","doi":"10.1093/plcell/koag190","DOIUrl":"10.1093/plcell/koag190","url":null,"abstract":"The evolutionarily conserved tradeoff between pathogen resistance and crop yield remains a major bottleneck in agricultural breeding. Although numerous genes governing disease resistance or growth productivity have been identified, the pleiotropic regulatory factors that harmonize these 2 agronomic traits remain largely elusive. Here, we report that the cotton (Gossypium hirsutum) chloroplast oxygen-evolving complex protein GhPsbO is dually modulated at both translational and transcriptional levels by the Verticillium dahliae effector Vd10375. Overexpression of GhPsbO enhances photosynthesis and accelerates lignin deposition to establish structural defense, thereby simultaneously improving crop yield and disease resistance in cotton. Unexpectedly, knockout of GhPsbO enhanced resistance to V. dahliae by elevating chloroplast-derived reactive oxygen accumulation and result in plant sacrifices. Consistently, transcriptional repression of GhPsbO by overexpression of the transcription factor GhMYB44 significantly improved cotton resistance, mirroring the GhPsbO knockout disease-resistant phenotype. Collectively, our findings uncover a regulatory module balancing plant growth and immunity, and provide a promising molecular strategy for breaking the growth-defense tradeoff to achieve sustainable crop production.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRP8 regulates chromatin organization to enforce transcriptional gene silencing independent of DNA methylation. PRP8调节染色质组织，使转录基因沉默独立于DNA甲基化。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag167

Lijuan Qi, Wanqing Zhuo, Guanyu Chen, Shaofeng Qu, Bo Wang, Yingjie Mi, Tonghui Li, Lingjiao Fan, Zhi Zhou, Liu-Min Fan, Xing-Wang Deng, Linhua Sun, Yan Xue, Weiqiang Qian

{"title":"PRP8 regulates chromatin organization to enforce transcriptional gene silencing independent of DNA methylation.","authors":"Lijuan Qi, Wanqing Zhuo, Guanyu Chen, Shaofeng Qu, Bo Wang, Yingjie Mi, Tonghui Li, Lingjiao Fan, Zhi Zhou, Liu-Min Fan, Xing-Wang Deng, Linhua Sun, Yan Xue, Weiqiang Qian","doi":"10.1093/plcell/koag167","DOIUrl":"10.1093/plcell/koag167","url":null,"abstract":"Transcriptional gene silencing (TGS) is critical in maintaining genome integrity in plants, during which DNA methylation plays a fundamental role. The establishment and maintenance of DNA methylation are well characterized, while the downstream events mediating transcriptional repression remain poorly understood. In this study, we performed a forward genetic screen for suppressors of hdp1-induced report gene silencing to identify components involved in TGS downstream of DNA methylation and identified Pre-mRNA processing factor 8 (PRP8), a well-known component of the spliceosome complex. Through comprehensive genetic analysis coupled with RNA sequencing and whole-genome bisulfite sequencing, we demonstrated that PRP8-mediated TGS of the transgenes along with a subset of endogenous genes and transposable elements in a DNA methylation-independent manner. Through high-throughput chromosome conformation capture (Hi-C) analysis, we demonstrated that PRP8 is required for maintaining pericentromeric heterochromatin interactions and higher-order chromatin organization. Further genetic analysis revealed that PRP8 acts synergistically with Arabidopsis MICRORCHIDIA (MORC) protein MORC6, HISTONE DEACETYLASE 6 (HDA6), MORPHEUS MOLECULE 1 (MOM1), and NUCLEAR RNA POLYMERASE D1B (NRPE1), suggesting its involvement in a previously uncharacterized TGS pathway. In summary, our findings establish PRP8 as a dual-function factor involved in both splicing and DNA methylation-independent TGS.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tyrosine-sulfated peptide-induced flavonol biosynthesis controls elongation and differentiation in Arabidopsis primary root. 酪氨酸-巯基肽诱导的黄酮醇生物合成控制拟南芥初生根的伸长和分化。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag180

Maria Florencia Ercoli, Alexandra M Shigenaga, Artur Teixeira de Araujo, Katherine B Louie, Benjamin P Bowen, Tracy S Weitz, Sangeetha Ramesh, Rashmi Jain, Trent R Northen, Pamela C Ronald

{"title":"Tyrosine-sulfated peptide-induced flavonol biosynthesis controls elongation and differentiation in Arabidopsis primary root.","authors":"Maria Florencia Ercoli, Alexandra M Shigenaga, Artur Teixeira de Araujo, Katherine B Louie, Benjamin P Bowen, Tracy S Weitz, Sangeetha Ramesh, Rashmi Jain, Trent R Northen, Pamela C Ronald","doi":"10.1093/plcell/koag180","DOIUrl":"10.1093/plcell/koag180","url":null,"abstract":"In Arabidopsis (Arabidopsis thaliana) roots, growth initiation and cessation are organized into distinct zones. How regulatory mechanisms are integrated to coordinate these processes and maintain proper growth progression over time remains poorly understood. Here, we demonstrate that the peptide hormone PLANT PEPTIDE CONTAINING SULFATED TYROSINE 1 (PSY1) promotes root growth by controlling cell elongation. Higher levels of PSY1 lead to longer differentiated cells with a shootward displacement of characteristics common to mature cells. PSY1 activates genes involved in the biosynthesis of flavonols, a group of plant-specific specialized metabolites. Consistent with these transcriptional changes, metabolomic analysis reveals an enrichment of diverse flavonol glycosides upon PSY1 treatment. Using genetic and chemical approaches, we show that PSY1-mediated flavonol accumulation is localized to the differentiation zone and is required for PSY1 function. PSY1 signaling in this zone is associated with a reduction of hydrogen peroxide accumulation and a decrease in auxin-induced gene expression. These findings support a model where PSY1 signals the developmental-specific accumulation of specialized metabolites to regulate the extent of cell elongation and progression to maturation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13354515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148265676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0