Plant Cell最新文献_第8页

VvFHY3 links auxin and endoplasmic reticulum stress to regulate grape anthocyanin biosynthesis at high temperatures. VvFHY3 将叶绿素和内质网应激联系起来，调节高温下葡萄花青素的生物合成。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae303

Yanzhao Sun, Yanyan Zheng, Wenyuan Wang, Heng Yao, Zain Ali, Mengwei Xiao, Zhaodong Ma, Jingjing Li, Wenfei Zhou, Jing Cui, Kun Yu, Yang Liu

{"title":"VvFHY3 links auxin and endoplasmic reticulum stress to regulate grape anthocyanin biosynthesis at high temperatures.","authors":"Yanzhao Sun, Yanyan Zheng, Wenyuan Wang, Heng Yao, Zain Ali, Mengwei Xiao, Zhaodong Ma, Jingjing Li, Wenfei Zhou, Jing Cui, Kun Yu, Yang Liu","doi":"10.1093/plcell/koae303","DOIUrl":"10.1093/plcell/koae303","url":null,"abstract":"Anthocyanins affect quality in fruits such as grape (Vitis vinifera). High temperatures reduce anthocyanin levels by suppressing the expression of anthocyanin biosynthesis genes and decreasing the biosynthetic rate. However, the regulatory mechanisms that coordinate these 2 processes remain largely unknown. In this study, we demonstrate that high-temperature-mediated inhibition of anthocyanin biosynthesis in grape berries depends on the auxin and endoplasmic reticulum (ER) stress pathways. Inactivation of these pathways restores anthocyanin accumulation under high temperatures. We identified and characterized FAR-RED ELONGATED HYPOCOTYL3 (FHY3), a high-temperature-modulated transcription factor that activates multiple anthocyanin biosynthesis genes by binding to their promoters. The auxin response factor VvARF3 interacts with VvFHY3 and represses its transactivation activity, antagonizing VvFHY3-induced anthocyanin biosynthesis. Additionally, we found that the ER stress sensor VvbZIP17 represses anthocyanin biosynthesis. VvFHY3 suppresses VvbZIP17 activity by directly binding to the VvbZIP17 promoter to repress its transcription and by physically interacting with VvbZIP17 to block its DNA binding ability. Furthermore, AUXIN RESPONSE FACTOR 3 (ARF3) interferes with the VvFHY3-VvbZIP17 interaction, releasing VvbZIP17 to activate the unfolded protein response and further suppress anthocyanin production. Our results unravel the VvARF3-VvFHY3-VvbZIP17 regulatory module, which links the auxin and ER stress pathways to coordinately repress anthocyanin structural gene expression and biosynthesis under high-temperature stress.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An InDel variant in the promoter of the NAC transcription factor MdNAC18.1 plays a major role in apple fruit ripening.

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koaf007

Qianyu Yue, Yinpeng Xie, Xinyue Yang, Yuxin Zhang, Zhongxing Li, Yunxiao Liu, Pengda Cheng, Ruiping Zhang, Yue Yu, Xiaofei Wang, Liao Liao, Yuepeng Han, Tao Zhao, Xuewei Li, Hengtao Zhang, Fengwang Ma, Qingmei Guan

{"title":"An InDel variant in the promoter of the NAC transcription factor MdNAC18.1 plays a major role in apple fruit ripening.","authors":"Qianyu Yue, Yinpeng Xie, Xinyue Yang, Yuxin Zhang, Zhongxing Li, Yunxiao Liu, Pengda Cheng, Ruiping Zhang, Yue Yu, Xiaofei Wang, Liao Liao, Yuepeng Han, Tao Zhao, Xuewei Li, Hengtao Zhang, Fengwang Ma, Qingmei Guan","doi":"10.1093/plcell/koaf007","DOIUrl":"10.1093/plcell/koaf007","url":null,"abstract":"A complex regulatory network governs fruit ripening, but natural variations and functional differentiation of fruit ripening genes remain largely unknown. Utilizing a genome-wide association study (GWAS), we identified the NAC family transcription factor MdNAC18.1, whose expression is closely associated with fruit ripening in apple (Malus × domestica Borkh.). MdNAC18.1 activated the transcription of genes related to fruit softening (Polygalacturonase, PG) and ethylene biosynthesis (1-aminocyclopropane-1-carboxylic acid synthase, ACS), thereby promoting fruit ripening of apple and tomato (Solanum lycopersicum). There were two single-nucleotide polymorphisms (SNP-1,545 and SNP-2,002) and a 58-bp insertion-deletion (InDel-58) in the promoter region of MdNAC18.1. Among these, InDel-58 serves as the main effector in activating the expression of MdNAC18.1 and driving fruit ripening. InDel-58 determines the binding affinity of the class D MADS-box protein AGAMOUS-LIKE 11 (MdAGL11), a negative regulator of fruit ripening. The InDel-58 deletion in the early-ripening genotype reduces the inhibitory effect of MdAGL11 on MdNAC18.1. Moreover, MdNAC18.1 and its homologous genes originated from a common ancestor across 61 angiosperms, with functional diversification attributed to tandem replications that occurred in basal angiosperms. In summary, our study revealed how a set of natural variations influence fruit ripening and explored the functional diversification of MdNAC18.1 during evolution.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"37 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143053179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tackling vascular wilt disease: A signaling cascade to strengthen the plant cell wall. 应对维管束枯萎病：强化植物细胞壁的信号级联

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae299

Shanice S Webster

引用次数: 0

The DENSE AND ERECT PANICLE1-GRAIN NUMBER ASSOCIATED module enhances rice yield by repressing CYTOKININ OXIDASE 2 expression. 结实直立的穗粒数相关模块通过抑制细胞分裂素氧化酶2的表达而提高水稻产量。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae309

Jinhui Zhang, Qibing Lin, Xin Wang, Jiale Shao, Yulong Ren, Xin Liu, Miao Feng, Shuai Li, Qi Sun, Sheng Luo, Bojuan Liu, Xinxin Xing, Yanqi Chang, Zhijun Cheng, Jianmin Wan

{"title":"The DENSE AND ERECT PANICLE1-GRAIN NUMBER ASSOCIATED module enhances rice yield by repressing CYTOKININ OXIDASE 2 expression.","authors":"Jinhui Zhang, Qibing Lin, Xin Wang, Jiale Shao, Yulong Ren, Xin Liu, Miao Feng, Shuai Li, Qi Sun, Sheng Luo, Bojuan Liu, Xinxin Xing, Yanqi Chang, Zhijun Cheng, Jianmin Wan","doi":"10.1093/plcell/koae309","DOIUrl":"10.1093/plcell/koae309","url":null,"abstract":"The phytohormone cytokinin (CK) positively regulates the activity of the inflorescence meristem (IM). Cytokinin oxidase 2/Grain number 1a (OsCKX2/Gn1a)-mediated degradation of CK in rice (Oryza sativa L.) negatively regulates panicle grain number, whereas DENSE AND ERECT PANICLE 1 (DEP1) positively regulates grain number per panicle (GNP). However, the detailed regulatory mechanism between DEP1 and OsCKX2 remains elusive. Here, we report the GRAS (GIBBERELLIN ACID INSENSITIVE, REPRESSOR OF GA1, and SCARECROW) transcription factor GRAIN NUMBER ASSOCIATED (GNA), previously thought to be involved in the Brassinosteroids (BRs) signaling pathway, directly inhibits OsCKX2 expression in the IM through a DEP1-GNA regulatory module. Overexpressing GNA leads to increased CK levels and consequently higher branch number, GNP, and yield. Both DEP1 and dep1 enhance the inhibitory effect of GNA on OsCKX2 expression through interacting with GNA. GNA promotes the translocation of DEP1 to the nucleus, while the gain-of-function mutant dep1 translocates into the nucleus in the absence of GNA. Our findings provide insight into the regulatory mechanism underlying OsCKX2 and a strategy to improve rice yield.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation of the transcription factor OsNAC29 by OsMAPK3 activates diterpenoid genes to promote rice immunity. OsMAPK3磷酸化转录因子OsNAC29激活二萜类基因，促进水稻免疫。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae320

Ling Lu, Jianbo Fang, Na Xia, Jing Zhang, Zhijuan Diao, Xun Wang, Yan Liu, Dingzhong Tang, Shengping Li

{"title":"Phosphorylation of the transcription factor OsNAC29 by OsMAPK3 activates diterpenoid genes to promote rice immunity.","authors":"Ling Lu, Jianbo Fang, Na Xia, Jing Zhang, Zhijuan Diao, Xun Wang, Yan Liu, Dingzhong Tang, Shengping Li","doi":"10.1093/plcell/koae320","DOIUrl":"10.1093/plcell/koae320","url":null,"abstract":"Well-conserved mitogen-activated protein kinase (MAPK) cascades are essential for orchestrating of a wide range of cellular processes in plants, including defense responses against pathogen attack. NAC transcription factors (TFs) play important roles in plant immunity, but their targets and how they are regulated remain largely unknown. Here, we identified the TF OsNAC29 as a key component of a MAPK signaling pathway involved in rice (Oryza sativa) disease resistance. OsNAC29 binds directly to CACGTG motifs in the promoters of OsTPS28 and OsCYP71Z2, which are crucial for the biosynthesis of the phytoalexin 5,10-diketo-casbene and consequently rice blast resistance. OsNAC29 positively regulates rice blast resistance by promoting the expression of of OsTPS28 and OsCYP71Z2, and the function of OsNAC29 is genetically dependent on OsCYP71Z2 and OsTPS28. Furthermore, OsNAC29 interacts with OsRACK1A and OsMAPK3/6 to form an immune complex; OsMAPK3 phosphorylates OsNAC29 at Thr304 to prevent its proteasome-mediated degradation and promote its function against rice blast fungus. Phosphorylation of OsNAC29 at Thr304 is induced upon Magnaporthe oryzae infection and chitin treatment. Our data demonstrate the positive role of the OsMAPK3-OsNAC29-OsTPS28/OsCYP71Z2 module in rice blast resistance, providing insights into the molecular regulatory network and fine-tuning of NAC TFs in rice immunity.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species- and organ-specific contribution of peroxisomal cinnamate:CoA ligases to benzoic and salicylic acid biosynthesis. 肉桂酸过氧化物酶体：辅酶a连接酶对苯甲酸和水杨酸生物合成的物种和器官特异性贡献。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae329

Yukang Wang, Huiying Miao, Jiehua Qiu, Menghui Liu, Gaochen Jin, Wenxuan Zhang, Shuyan Song, Pengxiang Fan, Xiufang Xin, Jianping Hu, Ran Li, Ronghui Pan

{"title":"Species- and organ-specific contribution of peroxisomal cinnamate:CoA ligases to benzoic and salicylic acid biosynthesis.","authors":"Yukang Wang, Huiying Miao, Jiehua Qiu, Menghui Liu, Gaochen Jin, Wenxuan Zhang, Shuyan Song, Pengxiang Fan, Xiufang Xin, Jianping Hu, Ran Li, Ronghui Pan","doi":"10.1093/plcell/koae329","DOIUrl":"10.1093/plcell/koae329","url":null,"abstract":"Salicylic acid (SA) is a prominent defense hormone whose basal level, organ-specific accumulation, and physiological role vary widely among plant species. Of the 2 known pathways of plant SA biosynthesis, the phenylalanine ammonia lyase (PAL) pathway is more ancient and universal but its biosynthetic and physiological roles in diverse plant species remain unclear. Studies in which the PAL pathway is specifically or completely inhibited, as well as a direct comparison of diverse species and different organs within the same species, are needed. To this end, we analyzed the PAL pathway in rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana), 2 distantly related model plants whose basal SA levels and distributions differ tremendously at the organism and tissue levels. Based on our recent identification of the rice peroxisomal cinnamate:CoA ligases (CNLs), we identified 2 peroxisomal CNLs from Arabidopsis and showed CNL as the most functionally specific enzyme among the known enzymes of the PAL pathway. We then revealed the species- and organ-specific contribution of the PAL pathway to benzoic and salicylic acid biosynthesis and clarified its physiological importance in rice and Arabidopsis. Our findings highlight the necessity to consider species and organ types in future SA-related studies and may help to breed new disease-resistant crops.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The time is ripe: Natural variability of MdNAC18.1 promoter plays a major role in fruit ripening.

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koaf004

Christian Damian Lorenzo

引用次数: 0

EARLY NODULIN93 acts via cytochrome c oxidase to alter respiratory ATP production and root growth in plants. 早期 NODULIN93 通过细胞色素 c 氧化酶改变植物呼吸 ATP 的产生和根的生长。

IF 1 1区生物学

Plant Cell Pub Date : 2024-11-02 DOI: 10.1093/plcell/koae242

Chun Pong Lee, Xuyen H Le, Ryan M R Gawryluk, José A Casaretto, Steven J Rothstein, A Harvey Millar

{"title":"EARLY NODULIN93 acts via cytochrome c oxidase to alter respiratory ATP production and root growth in plants.","authors":"Chun Pong Lee, Xuyen H Le, Ryan M R Gawryluk, José A Casaretto, Steven J Rothstein, A Harvey Millar","doi":"10.1093/plcell/koae242","DOIUrl":"10.1093/plcell/koae242","url":null,"abstract":"EARLY NODULIN 93 (ENOD93) has been genetically associated with biological nitrogen fixation in legumes and nitrogen use efficiency in cereals, but its precise function is unknown. We show that hidden Markov models define ENOD93 as a homolog of the N-terminal domain of RESPIRATORY SUPERCOMPLEX FACTOR 2 (RCF2). RCF2 regulates cytochrome oxidase (CIV), influencing the generation of a mitochondrial proton motive force in yeast (Saccharomyces cerevisiae). Knockout of ENOD93 in Arabidopsis (Arabidopsis thaliana) causes a short root phenotype and early flowering. ENOD93 is associated with a protein complex the size of CIV in mitochondria, but neither CIV abundance nor its activity changed in ruptured organelles of enod93. However, a progressive loss of ADP-dependent respiration rate was observed in intact enod93 mitochondria, which could be recovered in complemented lines. Mitochondrial membrane potential was higher in enod93 in a CIV-dependent manner, but ATP synthesis and ADP depletion rates progressively decreased. The respiration rate of whole enod93 seedlings was elevated, and root ADP content was nearly double that in wild type without a change in ATP content. We propose that ENOD93 and HYPOXIA-INDUCED GENE DOMAIN 2 (HIGD2) are the functional equivalent of yeast RCF2 but have remained undiscovered in many eukaryotic lineages because they are encoded by 2 distinct genes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":"4716-4731"},"PeriodicalIF":10.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Will the real Robert Hooke please stand up? 请真正的罗伯特-胡克站起来好吗？

IF 1 1区生物学

Plant Cell Pub Date : 2024-11-02 DOI: 10.1093/plcell/koae244

Winfried S Peters

引用次数: 0

The strigolactone receptor DWARF14 regulates flowering time in Arabidopsis. 绞股蓝内酯受体 DWARF14 调节拟南芥的开花时间。

IF 1 1区生物学

Plant Cell Pub Date : 2024-11-02 DOI: 10.1093/plcell/koae248

Jinrui Bai, Xi Lei, Jinlan Liu, Yi Huang, Lumei Bi, Yuehua Wang, Jindong Li, Haiyang Yu, Shixiang Yao, Li Chen, Bart J Janssen, Kimberley C Snowden, Meng Zhang, Ruifeng Yao

{"title":"The strigolactone receptor DWARF14 regulates flowering time in Arabidopsis.","authors":"Jinrui Bai, Xi Lei, Jinlan Liu, Yi Huang, Lumei Bi, Yuehua Wang, Jindong Li, Haiyang Yu, Shixiang Yao, Li Chen, Bart J Janssen, Kimberley C Snowden, Meng Zhang, Ruifeng Yao","doi":"10.1093/plcell/koae248","DOIUrl":"10.1093/plcell/koae248","url":null,"abstract":"Multiple plant hormones, including strigolactone (SL), play key roles in regulating flowering time. The Arabidopsis (Arabidopsis thaliana) DWARF14 (AtD14) receptor perceives SL and recruits F-box protein MORE AXILLARY GROWTH2 (MAX2) and the SUPPRESSOR OF MAX2-LIKE (SMXL) family proteins. These interactions lead to the degradation of the SMXL repressor proteins, thereby regulating shoot branching, leaf shape, and other developmental processes. However, the molecular mechanism by which SL regulates plant flowering remains elusive. Here, we demonstrate that intact strigolactone biosynthesis and signaling pathways are essential for normal flowering in Arabidopsis. Loss-of-function mutants in both SL biosynthesis (max3) and signaling (Atd14 and max2) pathways display earlier flowering, whereas the repressor triple mutant smxl6/7/8 (s678) exhibits the opposite phenotype. Retention of AtD14 in the cytoplasm leads to its inability to repress flowering. Moreover, we show that nuclear-localized AtD14 employs dual strategies to enhance the function of the AP2 transcription factor TARGET OF EAT1 (TOE1). AtD14 directly binds to TOE1 in an SL-dependent manner and stabilizes it. In addition, AtD14-mediated degradation of SMXL7 releases TOE1 from the repressor protein, allowing it to bind to and inhibit the FLOWERING LOCUS T (FT) promoter. This results in reduced FT transcription and delayed flowering. In summary, AtD14 perception of SL enables the transcription factor TOE1 to repress flowering, providing insights into hormonal control of plant flowering.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":"4752-4767"},"PeriodicalIF":10.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0