IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-17 DOI: 10.1093/plcell/koag289

Francisco J Escaray, Cristian J Antonelli, Begoña Balaguer, Carlos Vera-Antón, Joaquín Grau, José M Franco-Zorrilla, Pablo Vera

{"title":"A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris.","authors":"Francisco J Escaray, Cristian J Antonelli, Begoña Balaguer, Carlos Vera-Antón, Joaquín Grau, José M Franco-Zorrilla, Pablo Vera","doi":"10.1093/plcell/koag289","DOIUrl":"https://doi.org/10.1093/plcell/koag289","url":null,"abstract":"Laticifers, the coenocytic cells responsible for latex production, represent one of the most extreme examples of continuous and polarized growth in plants. Their unique architecture, extending over large distances, requires tight coordination between wall extensibility, cytoplasmic organization, and hormonal control. Here, we characterized the poor in latex 13 (pil13) mutant of Euphorbia lathyris which, similar to the previously described pil1 and pil6 mutants, is defective in laticifer expansion during post-embryonic phases of plant growth. Through comparative transcriptomic analyses of these three pil mutants, we identified a core set of 266 differentially expressed genes (DEGs) that define a transcriptomic signature associated with laticifers. We further identified a regulatory network linking two HD-Zip I transcription factors, HAT7 and ATHB13, with brassinosteroid (BR) signaling in the mediation of laticifer growth. This HAT7/ATHB13-BR module regulates the expression of laticifer DEGs involved in cell wall remodeling. Consequently, we found that laticifer cell walls are highly enriched in low-esterified homogalacturonan (HG) and branched rhamnogalacturonan-I (RG-I) pectins. Whole-mount immunolocalization revealed that laticifer cell walls exhibit a peculiar spatial segregation of pectic polysaccharides. These pectins follow a characteristic complementary, non-overlapping deposition pattern along the laticifer longitudinal axis, presumably conferring both elasticity and mechanical integrity. This modular pectin assembly appears compatible with the high flexibility and mechanical sinuosity required for growing of laticifers through host tissues, while accommodating internal turgor pressure changes derived from latex accumulation. Our findings define the laticifer as a cellular model in which wall remodeling, cell growth, turgor pressure, and hormonal signaling are mechanistically coupled.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149207650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RuBP availability contributes to the regulation of RUBISCO activity in Synechocystis. RuBP的有效性有助于调控RUBISCO的活性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-14 DOI: 10.1093/plcell/koag301

Luisa Wittemeier, Stéphanie Arrivault, Niels Neumann, Boris Maček, Nils Schmidt, Martin Hagemann, Joachim Kopka

{"title":"RuBP availability contributes to the regulation of RUBISCO activity in Synechocystis.","authors":"Luisa Wittemeier, Stéphanie Arrivault, Niels Neumann, Boris Maček, Nils Schmidt, Martin Hagemann, Joachim Kopka","doi":"10.1093/plcell/koag301","DOIUrl":"https://doi.org/10.1093/plcell/koag301","url":null,"abstract":"RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (RUBISCO) and the Calvin-Benson-Bassham (CBB) cycle assimilate most of the inorganic carbon on earth. Conserved CP12 proteins control diurnal C-assimilation of the CBB cycle. Their function originated in cyanobacteria that deactivate GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPDH) and PHOSPHORIBULOKINASE (PRK) during darkness by redox-regulated complex-formation with CP12. This mechanism indicates metabolic regulation of C-assimilation. We measured C-assimilation in vivo at the day/night transition by dynamic 13CO2 labeling and 1-C-positional monitoring of 13C-incorporation into 3-phosphoglycerate (3PGA) and aspartate of Synechocystis sp. PCC 6803 grown with high (5%) CO2 supply. Unlike wild type, a Δcp12 mutant accumulated ribulose-1,5-bisphosphate (RuBP) and assimilated 13CO2 in darkness. We validated these observations by proteome measurements and orthogonal quantifications of RUBISCO abundances and specific activities. Complementation of Δcp12 with native CP12 and point-mutants of its GAPDH- and PRK-binding domains demonstrated that defective complex formation is causal for aberrant C-assimilation during darkness and that RuBP accumulation correlates significantly with the time-shifted 13C-incorporation into 3PGA. RUBISCO carboxylase activity is present throughout the diurnal cycle and depends on CP12-controlled substrate availability. 13C-positional analyses suggest dysregulated RuBP regeneration in Δcp12 during darkness via futile metabolic cycling through gluconeogenesis and the oxidative pentose phosphate path. We propose that blocked regeneration and rapid depletion of RuBP contributes to the regulation of RUBISCO activity at day-night transitions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved DEAD-box RNA helicases regulate RNA silencing and plant immunity and are targeted by a powdery mildew effector. 保守的DEAD-box RNA解旋酶调节RNA沉默和植物免疫，是白粉病效应物的靶标。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-13 DOI: 10.1093/plcell/koag312

Hongcui Pei, Ziyu Chen, Jiali Li, Xinmeng Gui, Xue Li, Lei Feng, Tingting Du, Yaling Liu, Haibo Yang, Wenxi Huang, Juan Zhang, Dan Wang, Linqiu Yin, Yixuan Li, Ting Qi, Huaibing Jin, Xiaoming Zhang, Huishan Guo, Renchun Fan, Qian-Hua Shen, Yongli Qiao

{"title":"Conserved DEAD-box RNA helicases regulate RNA silencing and plant immunity and are targeted by a powdery mildew effector.","authors":"Hongcui Pei, Ziyu Chen, Jiali Li, Xinmeng Gui, Xue Li, Lei Feng, Tingting Du, Yaling Liu, Haibo Yang, Wenxi Huang, Juan Zhang, Dan Wang, Linqiu Yin, Yixuan Li, Ting Qi, Huaibing Jin, Xiaoming Zhang, Huishan Guo, Renchun Fan, Qian-Hua Shen, Yongli Qiao","doi":"10.1093/plcell/koag312","DOIUrl":"https://doi.org/10.1093/plcell/koag312","url":null,"abstract":"RNA silencing is a conserved defense mechanism that protects plants against viruses, bacteria, fungi, and oomycetes. Although diverse pathogens have evolved effectors that suppress host RNA silencing, whether powdery mildew fungi exploit this strategy and the underlying molecular mechanisms remain unknown. Here, we identify CSEP0004, a secreted effector from the barley powdery mildew fungus Blumeria hordei, as an RNA silencing suppressor that promotes fungal virulence. CSEP0004 physically interacts with the evolutionarily conserved DEAD-box RNA helicases Hordeum vulgare RNA helicase 37 (HvRH37) and HvRH52. Genetic analyses demonstrate that HvRH37 and HvRH52 positively regulate chitin-triggered reactive oxygen species production, resistance to B. hordei, and small RNA accumulation in barley, while their Arabidopsis homologs AtRH11/37/52 perform conserved functions in small RNA accumulation. Mechanistically, HvRH37 and HvRH52 associate with HvAGO1, a core component of the RNA-induced silencing complex, and function as biochemically active RNA helicases capable of binding and unwinding double-stranded RNA. CSEP0004 disrupts the HvRH37/52-AGO1 association and inhibits HvRH37/52 helicase activity in a dose-dependent manner. Together, our findings uncover a previously unrecognized mechanism by which a fungal effector suppresses host RNA silencing through targeting conserved DDX3-like DEAD-box RNA helicases, establishing these helicases as key regulators of RNAi-mediated immunity and identifying RNA helicase-dependent RNA remodeling as a critical regulatory node exploited by fungal pathogens.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LMBD2 interacts with components of the ECH/YIP complex and contributes to post-Golgi trafficking in Arabidopsis. LMBD2与ECH/YIP复合物的组分相互作用，并参与拟南芥的后高尔基转运。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-13 DOI: 10.1093/plcell/koag310

Meng Wang, Zongchang Xu, Natalie Hoffmann, Shuaiqiang Guo, Xuefeng Dou, Bin Wang, Yuyue Su, Yingyu Qu, Xinxin Song, Malcolm O'Neill, Peter Klemmed Bjørk, Staffan Persson, Yan Xu, Congpeng Wang, Dian Wang, Yingzhen Kong, Heather E McFarlane, Gongke Zhou

{"title":"LMBD2 interacts with components of the ECH/YIP complex and contributes to post-Golgi trafficking in Arabidopsis.","authors":"Meng Wang, Zongchang Xu, Natalie Hoffmann, Shuaiqiang Guo, Xuefeng Dou, Bin Wang, Yuyue Su, Yingyu Qu, Xinxin Song, Malcolm O'Neill, Peter Klemmed Bjørk, Staffan Persson, Yan Xu, Congpeng Wang, Dian Wang, Yingzhen Kong, Heather E McFarlane, Gongke Zhou","doi":"10.1093/plcell/koag310","DOIUrl":"https://doi.org/10.1093/plcell/koag310","url":null,"abstract":"The plant cell wall, composed primarily of polysaccharides, is essential for normal plant growth and development. Many of these polysaccharides are synthesized within and subsequently secreted via the endomembrane system. Mutations in genes including MURUS3 (MUR3), YPT/RAB GTPase-interacting protein 4 homologs (YIP4a/YIP4b), and ECHIDNA (ECH) result in dwarf plants, which is believed to be a consequence of impaired cell wall polysaccharide secretion. Here, we identify a LMBR1 (Limb Development Membrane Protein 1)-like membrane protein (LMBD2) and find that loss of LMBD2 suppresses the secretion and growth defects in the mur3, yip4a/yip4b, and ech mutants. LMBD2 localizes to the trans-Golgi apparatus and trans-Golgi network (TGN)/early endosome (EE), where it interacts with YIP4b and orchestrates TGN/EE-mediated trafficking. Furthermore, LMBD2 can competitively attenuate the interaction of YIP4b and ECH. Our findings establish LMBD2 as a component of endomembrane trafficking homeostasis in plant cells and offer new insights into endomembrane dynamics and their influence on plant development.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial and Single-Nucleus Transcriptomic Profiling Reveal Polarity and Intracellular Responses to Mitochondrial Dysfunction. 空间和单核转录组分析揭示极性和线粒体功能障碍的细胞内反应。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-08 DOI: 10.1093/plcell/koag308

Yanqiao Zhu, Minxuan Li, Jia Hao Li, Rijad Sarić, Feiran Han, Xinxin Zhou, Xiachao Wang, Yuhua Liu, Chi Zhang, Huixia Shou, Ghazanfar Abbas Khan, Mathew G Lewsey, James Whelan

{"title":"Spatial and Single-Nucleus Transcriptomic Profiling Reveal Polarity and Intracellular Responses to Mitochondrial Dysfunction.","authors":"Yanqiao Zhu, Minxuan Li, Jia Hao Li, Rijad Sarić, Feiran Han, Xinxin Zhou, Xiachao Wang, Yuhua Liu, Chi Zhang, Huixia Shou, Ghazanfar Abbas Khan, Mathew G Lewsey, James Whelan","doi":"10.1093/plcell/koag308","DOIUrl":"https://doi.org/10.1093/plcell/koag308","url":null,"abstract":"Organelle retrograde signaling studies have exclusively relied on bulk tissues, obscuring cell-type specificity, spatial organization, and heterogeneity within cell types. Here, we use single-nucleus and spatial RNA sequencing to define the cellular and spatial architecture of mitochondrial stress signaling in Arabidopsis thaliana. Inhibition of the mitochondrial electron transport chain by antimycin A and myxothiazol rapidly redistributed nuclei among transcriptional states within epidermal, leaf pavement, and mesophyll lineages, revealing that mitochondrial dysfunction reshapes cell identity trajectories rather than eliciting a uniform response. Stress-associated clusters were already present at low frequency in untreated samples, but the numbers of cells increased following stress treatments, suggesting that stress increases the proportion of nuclei occupying pre-existing transcriptional states. Gene-level analyses revealed distinct temporal dynamics and variable cell-state penetrance of mitochondrial stress markers and identified broadly responsive genes absent from earlier marker sets. Spatial transcriptomics resolved tissue-scale responses, spanning from pan-tissue induction to cell-type- and cluster-restricted activation, and uncovered pronounced adaxial-abaxial polarity in gene expression. Cell identity and spatial inferences were supported by extensive experimental validation using a custom 465-probe 10x Xenium panel and 52 promoter-GFP reporter lines. Together, these data provide a high-resolution framework for organelle-to-nucleus signaling and a resource of cell-type markers and spatial maps to dissect mitochondrial stress signaling and its integration with developmental and environmental pathways.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Turning off the antennae: NsrC suppresses phycobilisome biosynthesis during nitrogen starvation. 关闭触角：NsrC在氮饥饿期间抑制藻胆酶体的生物合成。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-07 DOI: 10.1093/plcell/koag309

Barbara Bourgade

引用次数: 0

A PSA4-Ycf4 complex promotes assembly of the photosystem I reaction center in Arabidopsis. PSA4-YCF4复合物促进拟南芥光系统I反应中心的组装。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag292

Fudan Gao, Qiang Xin, Lin Zhang, Lujuan Cai, Junxiang Ruan, Junhao Li, Tongtong Song, Weijie Chen, Jean-David Rochaix, Lianwei Peng

{"title":"A PSA4-Ycf4 complex promotes assembly of the photosystem I reaction center in Arabidopsis.","authors":"Fudan Gao, Qiang Xin, Lin Zhang, Lujuan Cai, Junxiang Ruan, Junhao Li, Tongtong Song, Weijie Chen, Jean-David Rochaix, Lianwei Peng","doi":"10.1093/plcell/koag292","DOIUrl":"10.1093/plcell/koag292","url":null,"abstract":"Photosystem I (PSI) is one of nature's most intricate and efficient nanomachines driving photosynthetic electron transfer. Its biogenesis requires a sophisticated, multi-step assembly pathway coordinated by a suite of nucleus- and plastid-encoded factors, yet the precise molecular mechanisms governing the earliest and most critical steps regarding the formation of its reaction center PsaA-PsaB remain poorly understood. Here, we have identified and characterized PSA4 (Photosystem I Assembly 4), a thylakoid membrane protein conserved in land plants and green algae. PSA4 physically interacts with the conserved assembly factor Ycf4 to form a functional complex. Loss of PSA4 drastically destabilizes Ycf4 and impairs the normal accumulation of PSI. In vivo pulse-labeling demonstrates that the PSA4-Ycf4 complex is specifically required for the efficient synthesis of the PSI reaction center subunits PsaA and PsaB, while their translation initiation appears unaffected. Topology analyses reveal that both the N- and C-termini of Ycf4 face the thylakoid lumen, where they directly interact with 4 proximal luminal loops of the PsaA/PsaB heterodimer. Based on these findings, we propose that PSA4 and Ycf4 form a dedicated complex that promotes the assembly of the PsaA/PsaB heterodimer during the early steps of PSI biogenesis in chloroplasts.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149001741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrate transiently rewires transcription in developing xylem of Populus wood to promote cell expansion via CRF4. 硝酸盐在杨木木质部发育过程中瞬时重组转录，通过CRF4促进细胞扩增。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag281

Shruti Choudhary, Anna Renström, Theerarat Kochakarn, Pal Miskolczi, Edoardo Piombo, Maxime Chantreau, Jeny Jose, Sandra Jämtgård, András Gorzsás, Johan Henriksson, Hannele Tuominen

{"title":"Nitrate transiently rewires transcription in developing xylem of Populus wood to promote cell expansion via CRF4.","authors":"Shruti Choudhary, Anna Renström, Theerarat Kochakarn, Pal Miskolczi, Edoardo Piombo, Maxime Chantreau, Jeny Jose, Sandra Jämtgård, András Gorzsás, Johan Henriksson, Hannele Tuominen","doi":"10.1093/plcell/koag281","DOIUrl":"10.1093/plcell/koag281","url":null,"abstract":"Nitrate functions both as a nutrient and a signaling molecule, but how nitrate is perceived and translated into developmental outputs within woody tissues is unknown. Here, we show that developing xylem in hybrid aspen (Populus tremula × P. tremuloides) mounts a rapid, cell-type-specific transcriptional response to nitrate that promotes xylem cell expansion. To isolate local nitrate signaling in wood-forming tissues, we delivered nitrate directly into the xylem sap and profiled transcriptomic responses from 2 to 48 h after treatment. Bulk RNA sequencing revealed a transient response peaking within 2 to 4 h. Single-cell RNA sequencing at the peak response resolved nitrate signaling across developing xylem cell types and showed that nitrate induced cell-type-specific programs linked to nitrogen transport, cell wall remodeling, and developmental progression, while repressing lignin-biosynthetic genes. These data indicate that nitrate acts transiently in wood-forming tissues to fine-tune early xylem development. Among nitrate-responsive transcription factors, CYTOKININ RESPONSE FACTOR 4 (CRF4) emerged as a candidate regulator of this response. CRF4 overexpression in hybrid aspen stimulated xylem cell expansion, whereas simultaneous knockout of CRF4 and its paralog, CRF3, significantly attenuated the nitrate-induced expansion. Together, our findings identify a local nitrate signaling module in developing wood and establish CRF4 as a component linking nitrate sensing to xylem cell expansion.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar coating the invasion: how smut fungi sweet-talk maize cell wall guards. 糖衣入侵：黑穗病真菌如何保护玉米细胞壁。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag271

Meenu Singla-Rastogi

引用次数: 0

The epigenetics of color swatches: histone deacetylation regulates green coloration in Chrysanthemum. 色板的表观遗传学：组蛋白去乙酰化调控菊花的绿色。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag297

Gunjan Sharma, Pei Qin Ng

引用次数: 0

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