IF 1 1区生物学

Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koaf048

Srinivas Kunta, Yardena Dahan, Shai Torgeman, Joanne Chory, Yogev Burko

{"title":"Species-specific PHYTOCHROME-INTERACTING FACTOR utilization in the plant morphogenetic response to environmental stimuli.","authors":"Srinivas Kunta, Yardena Dahan, Shai Torgeman, Joanne Chory, Yogev Burko","doi":"10.1093/plcell/koaf048","DOIUrl":"10.1093/plcell/koaf048","url":null,"abstract":"PHYTOCHROME-INTERACTING FACTORs (PIFs) regulate growth-related gene expression in response to environmental conditions. Among their diverse functions in regulating signal responses, PIFs play an important role in thermomorphogenesis (the response to increased ambient temperature) and in the shade avoidance response. While numerous studies have examined the varied roles of PIFs in Arabidopsis (Arabidopsis thaliana), their roles in crop plants remain poorly investigated. This study delves into the conservation of PIFs activity among species by examining their functions in tomato (Solanum lycopersicum) and comparing them to known PIF functions in Arabidopsis using single and higher-order mutants of tomato PIF genes (SlPIFs). We demonstrate that, in contrast to Arabidopsis, PIFs are not required for thermomorphogenesis-induced stem elongation in tomato. In addition, whereas Arabidopsis PIF8 has a minor effect on plant growth, tomato SlPIF8a plays a key role in the low red/far-red (R/FR) response. In contrast, SlPIF4 and SlPIF7s play minor roles in this process. We also investigated the tissue-specific low R/FR response in tomato seedlings and demonstrate that the aboveground organs exhibit a conserved response to low R/FR, which is regulated by SlPIFs. Our findings provide insights into PIF-mediated responses in crop plants, which may guide future breeding strategies to enhance yield under high planting densities.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges of translating Arabidopsis insights into crops. 将拟南芥研究成果转化为农作物所面临的挑战。

IF 1 1区生物学

Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koaf059

Cristóbal Uauy, Hilde Nelissen, Raquel Lía Chan, Johnathan A Napier, David Seung, Linsan Liu, Sarah M McKim

{"title":"Challenges of translating Arabidopsis insights into crops.","authors":"Cristóbal Uauy, Hilde Nelissen, Raquel Lía Chan, Johnathan A Napier, David Seung, Linsan Liu, Sarah M McKim","doi":"10.1093/plcell/koaf059","DOIUrl":"10.1093/plcell/koaf059","url":null,"abstract":"The significance of research conducted on Arabidopsis thaliana cannot be overstated. This focus issue showcases how insights from Arabidopsis have opened new areas of biology and directly advanced our understanding of crops. Here, experts intimately involved in bridging between Arabidopsis and crops share their perspectives on the challenges and opportunities for translation. First, we examine the translatability of genetic modules from Arabidopsis into maize, emphasizing the need to publish well-executed translational experiments, regardless of outcome. Second, we highlight the landmark success of HB4, the first GM wheat cultivar on the market, whose abiotic tolerance is borne from direct translation and based on strategies first outlined in Arabidopsis. Third, we discuss the decades-long journey to engineer oilseed crops capable of producing omega-3 fish oils, with Arabidopsis serving as a critical intermediary. Fourth, we explore how direct translation of starch synthesizing proteins characterized in Arabidopsis helped uncover novel mechanisms and functions in crops, with potential valuable applications. Finally, we illustrate how shared molecular factors between Arabidopsis and barley exhibit distinct molecular wiring as exemplified in cuticular and stomatal development. Together, these vignettes underscore the pivotal role of Arabidopsis as a foundational model plant while highlighting the challenges of translating discoveries into field-ready, commercial cultivars with enhanced knowledge-based traits.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis as a model for translational research. 拟南芥作为转化研究的模型。

IF 1 1区生物学

Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koae065

Anna E Yaschenko, Jose M Alonso, Anna N Stepanova

{"title":"Arabidopsis as a model for translational research.","authors":"Anna E Yaschenko, Jose M Alonso, Anna N Stepanova","doi":"10.1093/plcell/koae065","DOIUrl":"10.1093/plcell/koae065","url":null,"abstract":"Arabidopsis thaliana is currently the most-studied plant species on earth, with an unprecedented number of genetic, genomic, and molecular resources having been generated in this plant model. In the era of translating foundational discoveries to crops and beyond, we aimed to highlight the utility and challenges of using Arabidopsis as a reference for applied plant biology research, agricultural innovation, biotechnology, and medicine. We hope that this review will inspire the next generation of plant biologists to continue leveraging Arabidopsis as a robust and convenient experimental system to address fundamental and applied questions in biology. We aim to encourage laboratory and field scientists alike to take advantage of the vast Arabidopsis datasets, annotations, germplasm, constructs, methods, and molecular and computational tools in our pursuit to advance understanding of plant biology and help feed the world's growing population. We envision that the power of Arabidopsis-inspired biotechnologies and foundational discoveries will continue to fuel the development of resilient, high-yielding, nutritious plants for the betterment of plant and animal health and greater environmental sustainability.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raffinose family oligosaccharide hydrolysis by alkaline α-galactosidase CsAGA2 controls seed development in cucumber. 碱性α-半乳糖苷酶CsAGA2水解棉子糖家族寡糖控制黄瓜种子发育。

IF 1 1区生物学

Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koaf061

Huan Liu, Yuzi Shi, Yalong Zhao, Xuehui Yao, Jing Nie, Hujian Li, Yicong Guo, Dandan Yang, Qian Zhang, Zhen Yang, Xiaolei Sui

{"title":"Raffinose family oligosaccharide hydrolysis by alkaline α-galactosidase CsAGA2 controls seed development in cucumber.","authors":"Huan Liu, Yuzi Shi, Yalong Zhao, Xuehui Yao, Jing Nie, Hujian Li, Yicong Guo, Dandan Yang, Qian Zhang, Zhen Yang, Xiaolei Sui","doi":"10.1093/plcell/koaf061","DOIUrl":"10.1093/plcell/koaf061","url":null,"abstract":"Seed size and weight are pivotal agronomic traits that link plant sexual reproduction to subsequent seedling establishment. These 2 seed characteristics are affected by embryo development and sugar filling. However, the molecular mechanisms controlling sugar translocation and the timing between early embryo development and subsequent seed filling in cucumber (Cucumis sativus L.) remain poorly understood. Here, we report that alkaline α-galactosidase 2 (CsAGA2) is expressed in the placental vascular bundles and funicular phloem, and its encoded protein is responsible for the hydrolysis of raffinose family oligosaccharides (RFOs). We demonstrate that FUSCA3 (CsFUS3) activates, while Auxin Response Factor 9 (CsARF9) represses, CsAGA2 expression. The CsFUS3-Sucrose Non-fermenting-1 (Snf1)-Related Protein Kinase 1α1 (CsSnRK1α1) interaction further enhances CsAGA2 expression, while CsARF9 recruits the TOPLESS (CsTPL) corepressor and further weakens CsAGA2 expression. Transgenic CsAGA2-RNAi (RNA interference), CsFUS3-RNAi, csfus3-crispr, and CsARF9-OE (overexpression) lines showed severe seed abortion rates, likely caused by reduced sugar supply during embryo development and seed filling. These results demonstrate the critical collaborative roles of these proteins in delivering sugars for seed development. Our findings reveal that CsAGA2 is an essential enzyme that is switched on by CsFUS3 to precisely regulate embryo development and provide the vast quantities of sugars needed for seed filling in cucumber. As the seeds mature, CsARF9 dampens CsAGA2 expression to gradually reduce the sugar supply to seeds. Therefore, our data suggest that the CsFUS3 and CsARF9 sequentially regulate embryo development and seed filling via CsAGA2-mediated RFO catabolism in cucumber. Our findings provide a new strategy for manipulating seed filling to increase yield in the breeding process of seed crops.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense. 环状RNA circANK通过抑制microrna398b介导的防御来抑制水稻对细菌性枯萎病的抗性。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-09 DOI: 10.1093/plcell/koaf082

Xiaohui Liu, Peihong Wang, Sai Wang, Weixue Liao, Mingyan Ouyang, Sisi Lin, Rongpeng Lin, Panagiotis F Sarris, Vasiliki Michalopoulou, Xurui Feng, Zinan Zhang, Zhengyin Xu, Gongyou Chen, Bo Zhu

{"title":"The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense.","authors":"Xiaohui Liu, Peihong Wang, Sai Wang, Weixue Liao, Mingyan Ouyang, Sisi Lin, Rongpeng Lin, Panagiotis F Sarris, Vasiliki Michalopoulou, Xurui Feng, Zinan Zhang, Zhengyin Xu, Gongyou Chen, Bo Zhu","doi":"10.1093/plcell/koaf082","DOIUrl":"https://doi.org/10.1093/plcell/koaf082","url":null,"abstract":"Circular RNAs (circRNAs) are prevalent in eukaryotic cells and have been linked to disease progressions. Their unique circular structure and stability make them potential biomarkers and therapeutic targets. Compared to animal models, plant circRNA research is still in its infancy. The lack of effective tools to specifically knock down circRNAs without affecting host gene expression has slowed the progress of plant circRNA research. Here, we have developed a CRISPR-Cas13d tool that can specifically knock down circRNAs in plant systems, successfully achieving the targeted knockdown of circRNAs in rice (Oryza sativa). We further focused on Os-circANK (a circRNA derived from Ankyrin repeat-containing protein), a circRNA differentially expressed in rice upon pathogen infection. Physiological and biochemical experiments revealed that Os-circANK functions as a sponge for miR398b, suppressing the cleavage of Cu/Zn-Superoxidase Dismutase (CSD)1/CSD2/Copper Chaperone for Superoxide Dismutase (CCSD)/Superoxidase Dismutase (SODX) through ceRNA (competing endogenous RNA), leading to reduced ROS levels following Xanthomonas oryzae pv. oryzae (Xoo) infection and a negative regulation of rice resistance to bacterial blight. Our findings indicate Os-circANK inhibits rice resistance to bacterial blight via the microRNA398b(miR398b)/CSD/SOD pathway.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate. 重新排列的红苋菜染色体外环状DNA赋予对草甘膦和草铵膦的抗性。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf069

Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson

{"title":"A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.","authors":"Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson","doi":"10.1093/plcell/koaf069","DOIUrl":"10.1093/plcell/koaf069","url":null,"abstract":"Some herbicide-resistant weeds become resistant by generating additional copies of specific loci. For example, amplification of the locus encoding chloroplastic glutamine synthetase (GS2) produces herbicide resistance in the glufosinate-resistant Palmer amaranth (Amaranthus palmeri) accession MSR2. Previously, overamplification of the glyphosate-resistant gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Palmer amaranth was determined to be driven by an extrachromosomal circular DNA (eccDNA). Here, we describe a rearranged eccDNA that confers resistance to both glyphosate and glufosinate ammonium due to the coduplication of the native chromosomal regions that contain the genes that encode for these herbicides target proteins. In addition to EPSPS, the replicon carries 2 GS2 isoforms (GS2.1 and GS2.2) and other genes. MSR2 samples harbored eccDNA carrying only EPSPS coexisting with eccDNAs harboring both EPSPS and GS2. A second glufosinate-resistant Palmer amaranth accession (MSR1) showed distinct GS2.1 and GS2.2 amplification patterns from MSR2, suggesting the existence of diverse replicons in Palmer amaranth. EPSPS copy number was correlated with both GS2 isoforms copy number in MSR2, further supporting the coexistence of these genes in the same replicon. These findings shed light on the complexity of eccDNA formation in plant systems, with the collection and accumulation of extra pieces of DNA.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embracing the challenges ahead: a new chapter for the plant cell leadership. 迎接未来的挑战：植物细胞领导的新篇章。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf043

Pablo A Manavella

引用次数: 0

The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner. ATM激酶通过磷酸化依赖性物质稳定磷酸酶MKP2延缓拟南芥叶片衰老。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf066

Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li

{"title":"The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner.","authors":"Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li","doi":"10.1093/plcell/koaf066","DOIUrl":"10.1093/plcell/koaf066","url":null,"abstract":"Arabidopsis thaliana (Arabidopsis) Ataxia Telangiectasia Mutated (ATM) kinase plays a vital role in orchestrating leaf senescence; however, the precise mechanisms remain elusive. Here, our study demonstrates that ATM kinase activity is essential for mitigating age- and reactive oxygen species-induced senescence, as restoration of wild-type ATM reverses premature senescence in the atm mutant, while a kinase-dead ATM variant is ineffective. ATM physically interacts with and phosphorylates Mitogen-Activated Protein Kinase Phosphatase 2 (MKP2) to enhance stability under oxidative stress. Mutations in putative phosphorylation sites S15/154 on MKP2 disrupt its phosphorylation, stability, and senescence-delaying function. Moreover, mutation of mitogen-activated protein kinase 6, a downstream target of MKP2, alleviates the premature senescence phenotype of the atm mutant. Notably, the dual-specificity protein phosphatase 19 (HsDUSP19), a predicted human counter protein of MPK2, interacts with both ATM and HsATM and extends leaf longevity in Arabidopsis when overexpressed. These findings elucidate the molecular mechanisms underlying the role of ATM in leaf senescence and suggest that the ATM-MKP2 module is likely evolutionarily conserved in regulating the aging process across eukaryotes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content. 基因型环境互作对甘蓝型油菜种子含油量影响的遗传和转录组分析。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf062

Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo

{"title":"Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.","authors":"Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo","doi":"10.1093/plcell/koaf062","DOIUrl":"10.1093/plcell/koaf062","url":null,"abstract":"The molecular basis underlying crop traits is complex, with gene-by-environment interactions (GEIs) affecting phenotypes. However, quantitative trait nucleotide (QTN)-by-environment interactions (QEIs) and GEIs for seed oil content (SOC) in oil crops are rare. Here, we detected 11 environmentally specific and 10 stable additive QTNs and 11 QEIs for SOC in rapeseed (Brassica napus) using genome-wide association studies. Weighted gene co-expression network analysis identified 8 Environmental-Developmental Gene co-expression Modules for which the eigengenes correlated with SOC and the environment explained a large proportion of the variance in gene expression. By incorporating information from the multi-omics dataset, 17 candidate genes and 11 candidate GEIs for SOC were predicted. We mined 1 GEI candidate, LIGHT-DEPENDENT SHORT HYPOCOTYLS5 (LSH5), around the environmentally specific QTN qspOC.A02.1 and QEI qeOC.A02.1 detected by climatic indices as covariates. BnaA02.LSH5 was highly expressed in early seed development, and its expression varied significantly across planting sites, with a trend opposite to light-related climatic indices. The BnaA02.lsh5 and BnaC02.lsh5 double mutants had lower SOC, hypocotyl length, photosynthesis, and carbon- and energy-related metabolites compared with wild type. Moreover, BnaA02.LSH5 transcriptionally directly repressed BnaA02.pMDH2 in fatty acid β-oxidation and photosynthetic electron transport. We propose that BnaLSH5 affects seed oil accumulation in response to light intensity. This study provides a basis for creating high-oil germplasm that is adapted to specific environments.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis. 非传统的TPX2家族蛋白TPXL3调节α极光激酶在拟南芥纺锤体形态发生中的功能。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf065

Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu

{"title":"The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis.","authors":"Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu","doi":"10.1093/plcell/koaf065","DOIUrl":"10.1093/plcell/koaf065","url":null,"abstract":"Spindle assembly in vertebrates requires the Aurora kinase, which is targeted to microtubules and activated by TPX2 (Targeting Protein of XKLP2). In Arabidopsis (Arabidopsis thaliana), TPX2-LIKE 3 (TPXL3), but not the highly conserved TPX2, is essential. To test the hypothesis that TPXL3 regulates the function of α Aurora kinase in spindle assembly, we generated transgenic Arabidopsis lines expressing an artificial microRNA targeting TPXL3 mRNA (amiR-TPXL3). The resulting mutants exhibited growth retardation, which was linked to compromised TPXL3 expression. In the mutant cells, α Aurora was delocalized from spindle microtubules to the cytoplasm, and spindles were assembled without recognizable poles. A functional TPXL3-GFP fusion protein first prominently appeared on the prophase nuclear envelope. Then, TPXL3-GFP localized to spindle microtubules (primarily toward the spindle poles, like γ-tubulin), and finally to the re-forming nuclear envelope during telophase and cytokinesis. However, TPXL3 was absent from phragmoplast microtubules. In addition, we found that the TPXL3 N-terminal Aurora-binding motif, microtubule-binding domain, and importin-binding motif, but not the C-terminal segment, were required for its mitotic function. Expression of truncated TPXL3 variants enhanced the defects in spindle assembly and seedling growth of amiR-TPXL3 plants. Taken together, our findings uncovered the essential function of TPXL3, but not TPX2, in targeting and activating α Aurora kinase for spindle apparatus assembly in Arabidopsis.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant Cell最新文献