IF 1 1区生物学

Plant Cell Pub Date : 2025-04-03 DOI: 10.1093/plcell/koaf059

Cristóbal Uauy, Hilde Nelissen, Raquel Lía Chan, Johnathan A Napier, David Seung, Linsan Liu, Sarah M McKim

{"title":"Challenges of translating Arabidopsis insights into crops.","authors":"Cristóbal Uauy, Hilde Nelissen, Raquel Lía Chan, Johnathan A Napier, David Seung, Linsan Liu, Sarah M McKim","doi":"10.1093/plcell/koaf059","DOIUrl":"https://doi.org/10.1093/plcell/koaf059","url":null,"abstract":"The significance of research conducted on Arabidopsis thaliana cannot be overstated. This focus issue showcases how insights from Arabidopsis have opened new areas of biology and directly advanced our understanding of crops. Here, experts intimately involved in bridging between Arabidopsis and crops share their perspectives on the challenges and opportunities for translation. First, we examine the translatability of genetic modules from Arabidopsis into maize, emphasizing the need to publish well-executed translational experiments, regardless of outcome. Second, we highlight the landmark success of HB4, the first GM wheat cultivar on the market, whose abiotic tolerance is borne from direct translation and based on strategies first outlined in Arabidopsis. Third, we discuss the decades-long journey to engineer oilseed crops capable of producing omega-3 fish oils, with Arabidopsis serving as a critical intermediary. Fourth, we explore how direct translation of starch synthesizing proteins characterised in Arabidopsis helped uncover novel mechanisms and functions in crops, with potential valuable applications. Finally, we illustrate how shared molecular factors between Arabidopsis and barley exhibit distinct molecular wiring as exemplified in cuticular and stomatal development. Together, these vignettes underscore the pivotal role of Arabidopsis as a foundational model plant while highlighting the challenges of translating discoveries into field-ready, commercial cultivars with enhanced knowledge-based traits.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using chimeric rice proteins to make heads or tails of the function of repetitive elements in Gγ subunits. 利用嵌合水稻蛋白来了解 Gγ 亚基中重复元件的功能。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-03 DOI: 10.1093/plcell/koaf080

Julie Robinson

引用次数: 0

The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf066

Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li

{"title":"The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner.","authors":"Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li","doi":"10.1093/plcell/koaf066","DOIUrl":"10.1093/plcell/koaf066","url":null,"abstract":"Arabidopsis thaliana (Arabidopsis) Ataxia Telangiectasia Mutated (ATM) kinase plays a vital role in orchestrating leaf senescence; however, the precise mechanisms remain elusive. Here, our study demonstrates that ATM kinase activity is essential for mitigating age- and reactive oxygen species-induced senescence, as restoration of wild-type ATM reverses premature senescence in the atm mutant, while a kinase-dead ATM variant is ineffective. ATM physically interacts with and phosphorylates Mitogen-Activated Protein Kinase Phosphatase 2 (MKP2) to enhance stability under oxidative stress. Mutations in putative phosphorylation sites S15/154 on MKP2 disrupt its phosphorylation, stability, and senescence-delaying function. Moreover, mutation of mitogen-activated protein kinase 6, a downstream target of MKP2, alleviates the premature senescence phenotype of the atm mutant. Notably, the dual-specificity protein phosphatase 19 (HsDUSP19), a predicted human counter protein of MPK2, interacts with both ATM and HsATM and extends leaf longevity in Arabidopsis when overexpressed. These findings elucidate the molecular mechanisms underlying the role of ATM in leaf senescence and suggest that the ATM-MKP2 module is likely evolutionarily conserved in regulating the aging process across eukaryotes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf062

Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo

{"title":"Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.","authors":"Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo","doi":"10.1093/plcell/koaf062","DOIUrl":"10.1093/plcell/koaf062","url":null,"abstract":"The molecular basis underlying crop traits is complex, with gene-by-environment interactions (GEIs) affecting phenotypes. However, quantitative trait nucleotide (QTN)-by-environment interactions (QEIs) and GEIs for seed oil content (SOC) in oil crops are rare. Here, we detected 11 environmentally specific and 10 stable additive QTNs and 11 QEIs for SOC in rapeseed (Brassica napus) using genome-wide association studies. Weighted gene co-expression network analysis identified 8 Environmental-Developmental Gene co-expression Modules for which the eigengenes correlated with SOC and the environment explained a large proportion of the variance in gene expression. By incorporating information from the multi-omics dataset, 17 candidate genes and 11 candidate GEIs for SOC were predicted. We mined 1 GEI candidate, LIGHT-DEPENDENT SHORT HYPOCOTYLS5 (LSH5), around the environmentally specific QTN qspOC.A02.1 and QEI qeOC.A02.1 detected by climatic indices as covariates. BnaA02.LSH5 was highly expressed in early seed development, and its expression varied significantly across planting sites, with a trend opposite to light-related climatic indices. The BnaA02.lsh5 and BnaC02.lsh5 double mutants had lower SOC, hypocotyl length, photosynthesis, and carbon- and energy-related metabolites compared with wild type. Moreover, BnaA02.LSH5 transcriptionally directly repressed BnaA02.pMDH2 in fatty acid β-oxidation and photosynthetic electron transport. We propose that BnaLSH5 affects seed oil accumulation in response to light intensity. This study provides a basis for creating high-oil germplasm that is adapted to specific environments.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bending over backwards for soil emergence: KIPK and KIPK-LIKE1 regulate hypocotyl gravitropic growth.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf073

Gwendolyn K Kirschner

引用次数: 0

Dawning aurora: TPXL3 activates α-Aurora kinase and regulates mitotic spindle morphogenesis.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf074

Renuka Kolli

引用次数: 0

A CRY1-HY5-MYB signaling cascade fine-tunes guard cell reactive oxygen species levels and triggers stomatal opening.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf064

Yuankai Chang, Mianmian Shi, Xiao Wang, Hui Cheng, Junli Zhang, Hongrui Liu, Huiruo Wu, Xiaobin Ou, Ke Yu, Xuebin Zhang, Brad Day, Chen Miao, Yi Zhao, Kun Jiang

{"title":"A CRY1-HY5-MYB signaling cascade fine-tunes guard cell reactive oxygen species levels and triggers stomatal opening.","authors":"Yuankai Chang, Mianmian Shi, Xiao Wang, Hui Cheng, Junli Zhang, Hongrui Liu, Huiruo Wu, Xiaobin Ou, Ke Yu, Xuebin Zhang, Brad Day, Chen Miao, Yi Zhao, Kun Jiang","doi":"10.1093/plcell/koaf064","DOIUrl":"10.1093/plcell/koaf064","url":null,"abstract":"Stomatal opening facilitates CO2 uptake and causes water loss via transpiration. Compared with the considerable progress made toward understanding phototropin-mediated blue light (BL) signaling in guard cells, the significance of cryptochromes (CRYs) in stomatal opening and their downstream elements remain largely unknown. Here, we show that 3 homologous MYB transcription factor genes, namely MYB11, MYB12, and MYB111, are rapidly transactivated in guard cells during the dark-to-light transition in Arabidopsis (Arabidopsis thaliana). Genetic characterization of myb mutants demonstrates that these proteins specifically mediate light-induced stomatal opening by promoting local flavonol accumulation, thereby controlling reactive oxygen species homeostasis in guard cells. In response to light, activation of the plasma membrane H+-ATPase is inhibited in the myb11 myb12 myb111 triple mutant, compromising transmembrane K+ influx in the mutant guard cells. Furthermore, we demonstrate that MYB11/12/111 expression in guard cells upon illumination is induced by a CRY1-specific signaling cascade involving ELONGATED HYPOCOTYL 5 (HY5), a direct transcriptional activator of these MYBs. Overall, our work reveals a mechanism by which the CRY1-HY5-MYB module facilitates light-induced stomatal opening, providing evidence that flavonoid metabolism in guard cells is crucial for plant stress tolerance.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Palmer amaranth's arsenal: Rearrangement of eccDNA provides dual herbicide resistance in Amaranthus palmeri.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf077

Yu-Hung Hung

引用次数: 0

The UV-B photoreceptor UVR8 interacts with the LOX1 enzyme to promote stomatal closure through the LOX-derived oxylipin pathway.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf060

Yan Liu, Jue Wang, Xiaotian Liu, Ting Liao, Hui Ren, Liang Liu, Xi Huang

{"title":"The UV-B photoreceptor UVR8 interacts with the LOX1 enzyme to promote stomatal closure through the LOX-derived oxylipin pathway.","authors":"Yan Liu, Jue Wang, Xiaotian Liu, Ting Liao, Hui Ren, Liang Liu, Xi Huang","doi":"10.1093/plcell/koaf060","DOIUrl":"10.1093/plcell/koaf060","url":null,"abstract":"Ultraviolet-B (UV-B) light-induced stomatal closure requires the photoreceptor UV RESISTANCE LOCUS 8 (UVR8) and nitric oxide (NO). However, the signaling pathways by which UV-B light regulates stomatal closure remain elusive. Here, we reveal that UVR8 signaling in the epidermis mediates stomatal closure in a tissue-specific manner in Arabidopsis (Arabidopsis thaliana). UV-B light promotes PHOSPHOLIPASE 1 (PLIP1)/PLIP3-mediated linoleic acid and α-linolenic acid accumulation and induces LIPOXYGENASE 1 (LOX1) expression. LOX1, which catabolizes linoleic acid and α-linolenic acid to produce oxylipin derivatives, acts downstream of UVR8 and upstream of the salicylic acid (SA) pathway associated with stomatal defense. Photoactivated UVR8 interacts with LOX1 and enhances its activity. Protein crystallography demonstrates that A. thaliana LOX1 and its ortholog in soybean (Glycine max) share overall structural similarity and conserved residues in the oxygen cavity, substrate cavity, and metal-binding site that are required for 9-LOX activity. The disruption of UVR8-LOX1 contact sites near the LOX1 oxygen and substrate cavities prevents UVR8-enhanced LOX1 activity and compromises stomatal closure upon UV-B exposure. Overall, our study uncovers a noncanonical UV-B signaling module, consisting of the UVR8 photoreceptor and the cytoplasmic lipoxygenase, that mediates stomatal responses to UV-B light.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complex immune puzzle: A deeper dive into the MORC1-mediated broad-spectrum defense signaling pathway.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf075

Meenu Singla-Rastogi

引用次数: 0

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