IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-06 DOI: 10.1093/plcell/koag131

Xin Jin, Wen Li, Xinyue Zhang, Fu-Qing Wu, Yupeng Wang, Yimin Ling, Jie Wang, Zhiwei Li, Lizhuo Ma, Xin Liu, Minxi Wu, Limin Zhang, Xudong Zhu, Ming Yu, Qiyu Yang, Yulong Ren, Cailin Lei, Qibin Lin, Zhijun Cheng, Zhichao Zhao, Xiuping Guo, Xin Wang, Shirong Zhou, Shanshan Zhu, Jianmin Wan

{"title":"The ELD4-OsPRR95 module represses OsMADS51 in regulating rice heading date.","authors":"Xin Jin, Wen Li, Xinyue Zhang, Fu-Qing Wu, Yupeng Wang, Yimin Ling, Jie Wang, Zhiwei Li, Lizhuo Ma, Xin Liu, Minxi Wu, Limin Zhang, Xudong Zhu, Ming Yu, Qiyu Yang, Yulong Ren, Cailin Lei, Qibin Lin, Zhijun Cheng, Zhichao Zhao, Xiuping Guo, Xin Wang, Shirong Zhou, Shanshan Zhu, Jianmin Wan","doi":"10.1093/plcell/koag131","DOIUrl":"https://doi.org/10.1093/plcell/koag131","url":null,"abstract":"Heading date is a key agronomic trait that affects crop yield and regional adaptability. In this study, we identified a rice (Oryza sativa) early heading mutant and cloned the causal heading inhibitor gene EARLY HEADING AT LONG DAY 4 (ELD4) using the MutMap method. The eld4 CRISPR mutants and ELD4 RNAi plants flowered earlier than the wild type under natural long-day (NLD) conditions. ELD4 is a zinc finger transcription factor that localizes in the nucleus. Biochemical and genetic evidence suggests that ELD4 physically interacts with the pseudo-response regulator (PRR) protein OsPRR95. The Osprr95 mutant also exhibits an earlier heading phenotype under NLD. ELD4 and OsPRR95 co-regulate heading date by directly binding to the promoter and the first intron of OsMADS51, which promotes heading date. Moreover, electrophoretic mobility shift and luciferase complementation assays showed that ELD4 enhances OsPRR95 binding to the first intron of OsMADS51. These results suggest that the ELD4-OsPRR95 module directly represses OsMADS51 in regulating flowering time. Haplotype analysis reveals that OsPRR95 haplotype 3, which is geographically distributed in the north, exhibits a shorter heading date than haplotype 1 found in the south. This indicates that selection at the OsPRR95 locus has enhanced the regional adaptability of rice.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Gα protein FveGPA1 regulates inflorescence architecture via the FveBRI1-mediated brassinosteroid pathway in strawberry. 草莓中Gα蛋白fvepa1通过fvebri1介导的油菜素内酯途径调控花序结构。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-06 DOI: 10.1093/plcell/koag134

Zhenying Zhu, Yunming Zhang, Yafan Han, Shaoqiang Hu, Guanghui Zheng, Yating Wu, Pengbo Xu, Hongli Lian, Chunying Kang

{"title":"The Gα protein FveGPA1 regulates inflorescence architecture via the FveBRI1-mediated brassinosteroid pathway in strawberry.","authors":"Zhenying Zhu, Yunming Zhang, Yafan Han, Shaoqiang Hu, Guanghui Zheng, Yating Wu, Pengbo Xu, Hongli Lian, Chunying Kang","doi":"10.1093/plcell/koag134","DOIUrl":"https://doi.org/10.1093/plcell/koag134","url":null,"abstract":"Inflorescence architecture determines fruit number and size, making it a critical agricultural trait. In strawberry, inflorescences display distinct branching patterns that correlate with fruit size uniformity, but the underlying genetic mechanisms remain poorly understood. Here, we identified four basal-branching inflorescence mutants (bbi1-1, bbi1-2, bbi2-1, and bbi2-2) in woodland strawberry (Fragaria vesca), whose inflorescences are partially basal-branching compared to the upper-branching inflorescences of the wild type. The heterotrimeric G-protein α-subunit gene FveGPA1 is responsible for the defects in bbi1-1 and bbi1-2, whose phenotypes are further enhanced by a mutation in the γ-subunit gene FveAGG3. The brassinosteroid (BR) receptor gene FveBRI1 is the causal gene for the bbi2-1 and bbi2-2 mutants. Notably, bbi1 bbi2 double mutants produce exclusively basal-branching inflorescences, a more severe phenotype than single mutants, suggesting a synergistic interaction between FveGPA1 and FveBRI1. Gene expression analysis, BR sensitivity assays, and BR metabolite quantification, together with the observed rescue of the bbi1 phenotype through loss-of-function of the BR metabolic enzyme CYP734A129, collectively indicate that FveGPA1 regulates inflorescence architecture via the BR signaling pathway. This phenotypic change is driven by differential elongation of the peduncle and pedicel due to cell division and expansion. Our findings reveal a molecular framework for inflorescence internode elongation mediated by the heterotrimeric G-protein and BR pathways and provide a theoretical basis for optimizing strawberry inflorescence architecture.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A wheat MYB transcription factor activates defense by repressing TaSIZ1 in response to branched-chain amino acid accumulation. 小麦MYB转录因子通过抑制TaSIZ1激活防御，以响应支链氨基酸积累。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-06 DOI: 10.1093/plcell/koag135

Swathy Puthanvila Surendrababu, Loizos Savva, Andrey V Korolev, Lionel Hill, Gerhard Saalbach, Diane G O Saunders

{"title":"A wheat MYB transcription factor activates defense by repressing TaSIZ1 in response to branched-chain amino acid accumulation.","authors":"Swathy Puthanvila Surendrababu, Loizos Savva, Andrey V Korolev, Lionel Hill, Gerhard Saalbach, Diane G O Saunders","doi":"10.1093/plcell/koag135","DOIUrl":"https://doi.org/10.1093/plcell/koag135","url":null,"abstract":"Branched-chain amino acid (BCAA) accumulation has been linked to induction of salicylic acid (SA)-related defense responses in wheat (Triticum aestivum). Here, we explored whether the underlying mechanism might involve a wheat ortholog of SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1), a critical SA regulator in other systems. We found that TaSIZ1 was significantly repressed in mutant plants disrupted in the BCAA aminotransferase gene TaBCAT1; these plants accumulate enhanced levels of BCAAs and SA. Overexpressing TaSIZ1 suppressed SA-hyperaccumulation in TaBCAT1 mutants and treating wild-type plants with the BCAA Leu repressed TaSIZ1 expression. Thus, TaSIZ1 is responsive to BCAA levels and influences SA accumulation, consistent with a role linking BCAA and SA in defense. Nuclear proteomic analysis of the TaBCAT1 mutant identified transcriptional regulators that could be modifying BCAA-responsive TaSIZ1 expression. This included an ortholog of the Arabidopsis thaliana trihelix transcription repressor 6b-INTERACTING PROTEIN-LIKE1 (ASIL1) with a potential binding site in the TaSIZ1 promoter. Further work showed that TaASIL1 bound to the TaSIZ1 promoter. Also, disrupting TaASIL1 function inhibited Leu-dependent TaSIZ1 repression. Based on these data, we propose that elevated BCAA levels such as those arising during pathogen attack activate TaASIL1, which represses TaSIZ1, thereby promoting SA accumulation and SA-mediated defense in wheat.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Every angle covered: OsWRKY36 regulates leaf inclination in rice. 覆盖的每个角度：OsWRKY36调节水稻叶片倾角。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-06 DOI: 10.1093/plcell/koag129

Nataliia Konstantinova

引用次数: 0

Alternative polyadenylation dynamics shape pollen development at single-cell resolution. 在单细胞分辨率下，可选择的聚腺苷化动力学影响花粉的发育。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-05 DOI: 10.1093/plcell/koag128

Ziwei Zhao, Jiawen Zhou, Danhui Zhao, Xiaojuan Lu, Qingshun Q Li

{"title":"Alternative polyadenylation dynamics shape pollen development at single-cell resolution.","authors":"Ziwei Zhao, Jiawen Zhou, Danhui Zhao, Xiaojuan Lu, Qingshun Q Li","doi":"10.1093/plcell/koag128","DOIUrl":"https://doi.org/10.1093/plcell/koag128","url":null,"abstract":"Alternative polyadenylation (APA) is a widespread co-transcriptional mechanism that regulates gene expression in growth, development and environmental responses. Pollen development is essential for the reproductive success of flowering plants, yet the contribution of APA to this process remains poorly understood. Here, we combine bulk RNA-seq in multiple tissues with single-nucleus transcriptomics across pollen developmental stages to systematically characterize APA dynamics during Arabidopsis thaliana pollen development. We show that mature pollen exhibits the most tissue-specific APA profile among the examined tissues, characterized by widespread 3' untranslated region (3' UTR) shortening. At single-nucleus resolution, APA patterns display pronounced temporal and cell-type specificity, particularly during the transition from bicellular to tricellular pollen and during vegetative nucleus maturation. Sperm nuclei exhibit the most distinct poly(A) site usage patterns. Moreover, genetic analyses of representative genes showed that altered poly(A) site usage is associated with changes in transcript abundance and pollen development phenotypes. Consistent with these observations, in vivo reporter assays showed that 3' UTR configurations are sufficient to modulate gene expression at the transcript level. Together, our study establishes APA as a structured co-transcriptional regulatory layer during pollen development and provides a framework for understanding 3' end-mediated gene regulation in male gametophytes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147841454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The heat is on: HSFA1 drives regeneration after wounding. 热的是：HSFA1驱动损伤后的再生。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-04 DOI: 10.1093/plcell/koag132

Luciano M Di Fino

引用次数: 0

Build me up to break me down: Mapping grain development and germination in barley over time and space. 建立我，摧毁我：绘制大麦在时间和空间上的谷物发育和发芽图。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-05-04 DOI: 10.1093/plcell/koag130

Pablo González-Suárez, Alexa-Maria Wangler

引用次数: 0

A GA-independent, membrane-associated pathway regulates DELLA degradation during carbon/nitrogen stress. 在碳/氮胁迫下，一种不依赖ga的膜相关途径调节DELLA降解。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-04-03 DOI: 10.1093/plcell/koag037

Ju-Chen Chia

引用次数: 0

Better together: the transcription factor MUTE aggregates into nuclear condensates to regulate stomatal development in maize. 更好地在一起：转录因子MUTE聚集成核凝聚体，调节玉米气孔发育。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-04-03 DOI: 10.1093/plcell/koag050

Pablo González-Suárez

引用次数: 0

DNA repair under heat: DNA polymerase λ modulates heat stress-induced mutagenesis in plants. 高温下的DNA修复：DNA聚合酶λ调节植物热胁迫诱导的诱变。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-04-03 DOI: 10.1093/plcell/koag090

Clair M Wootan, John Lutterman, Nathan Springer, Xiaosa Xu, Feng Zhang

{"title":"DNA repair under heat: DNA polymerase λ modulates heat stress-induced mutagenesis in plants.","authors":"Clair M Wootan, John Lutterman, Nathan Springer, Xiaosa Xu, Feng Zhang","doi":"10.1093/plcell/koag090","DOIUrl":"10.1093/plcell/koag090","url":null,"abstract":"Mutation rates often rise under environmental stress, a process known as stress-induced mutagenesis. Among abiotic factors, heat stress is a potent driver that elevates mutation rates and enhances genetic variation, yet the underlying mechanisms in eukaryotes remain unclear. Here, we investigated how heat stress influences DNA repair and mutagenesis both locally and globally in Arabidopsis thaliana using CRISPR-Cas9-induced DNA breaks, whole genome sequencing, and single-cell transcriptomic analysis. Heat stress greatly enhanced CRISPR editing efficiency across different chromatin contexts, reaching up to a 29.9-fold increase in heterochromatic regions. Moreover, heat stress consistently shifted repair outcomes toward one base pair insertions, independent of chromatin state. We identified a heat-inducible, error-prone DNA polymerase, DNA polymerase λ (DNA Polλ), as the key mediator of these repair profile changes. Genome-wide analyses of somatic mutations further revealed that the heat-induced increase in mutagenesis also depends on DNA Polλ. Single-cell transcriptomic profiling showed that DNA Polλ expression is tightly regulated and enriched in the central zone of the shoot apical meristem. Such tissue-specific patterns suggest that DNA Polλ serves dual functions: maintaining genome integrity in essential stem cell populations while also enabling stress-induced mutagenesis that can be transmitted to progeny.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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