IF 1 1区生物学

Plant Cell Pub Date : 2024-09-05 DOI: 10.1093/plcell/koae248

Jinrui Bai, Xi Lei, Jinlan Liu, Yi Huang, Lumei Bi, Yuehua Wang, Jindong Li, Haiyang Yu, Shixiang Yao, Li Chen, Bart J Janssen, Kimberley C Snowden, Meng Zhang, Ruifeng Yao

{"title":"The strigolactone receptor DWARF14 regulates flowering time in Arabidopsis.","authors":"Jinrui Bai, Xi Lei, Jinlan Liu, Yi Huang, Lumei Bi, Yuehua Wang, Jindong Li, Haiyang Yu, Shixiang Yao, Li Chen, Bart J Janssen, Kimberley C Snowden, Meng Zhang, Ruifeng Yao","doi":"10.1093/plcell/koae248","DOIUrl":"https://doi.org/10.1093/plcell/koae248","url":null,"abstract":"Multiple plant hormones, including strigolactone (SL), play key roles in regulating flowering time. The Arabidopsis (Arabidopsis thaliana) DWARF14 (AtD14) receptor perceives SL and recruits F-box protein MORE AXILLARY GROWTH2 (MAX2) and the SUPPRESSOR OF MAX2-LIKE (SMXL) family proteins. These interactions lead to the degradation of the SMXL repressor proteins, thereby regulating shoot branching, leaf shape, and other developmental processes. However, the molecular mechanism by which SL regulates plant flowering remains elusive. Here, we demonstrate that intact strigolactone biosynthesis and signaling pathways are essential for normal flowering in Arabidopsis. Loss-of-function mutants in both SL biosynthesis (max3) and signaling (Atd14 and max2) pathways display earlier flowering, whereas the repressor triple mutant smxl6/7/8 (s678) exhibits the opposite phenotype. Retention of AtD14 in the cytoplasm leads to its inability to repress flowering. Moreover, we show that nuclear-localized AtD14 employs dual strategies to enhance the function of the AP2 transcription factor TARGET OF EAT1 (TOE1). AtD14 directly binds to TOE1 in an SL-dependent manner and stabilizes it. In addition, AtD14-mediated degradation of SMXL7 releases TOE1 from the repressor protein, allowing it to bind to and inhibit the FLOWERING LOCUS T (FT) promoter. This results in reduced FT transcription and delayed flowering. In summary, AtD14 perception of SL enables the transcription factor TOE1 to repress flowering, providing insights into hormonal control of plant flowering.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small protein, big effects: ENOD93 alters mitochondrial ATP production to favor nitrogen assimilation in plants. 小蛋白，大作用：ENOD93 改变线粒体 ATP 的产生，有利于植物的氮同化。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-05 DOI: 10.1093/plcell/koae247

Renuka Kolli

引用次数: 0

Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light. 磷酸化转录因子 PuHB40 在暴露于强光的梨中介导 ROS 依赖性花青素生物合成。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae167

Lu Zhang, Lu Wang, Yongchen Fang, Yuhao Gao, Shulin Yang, Jun Su, Junbei Ni, Yuanwen Teng, Songling Bai

{"title":"Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light.","authors":"Lu Zhang, Lu Wang, Yongchen Fang, Yuhao Gao, Shulin Yang, Jun Su, Junbei Ni, Yuanwen Teng, Songling Bai","doi":"10.1093/plcell/koae167","DOIUrl":"10.1093/plcell/koae167","url":null,"abstract":"Plants are increasingly vulnerable to environmental stresses because of global warming and climate change. Stress-induced reactive oxygen species (ROS) accumulation results in plant cell damage, even cell death. Anthocyanins are important antioxidants that scavenge ROS to maintain ROS homeostasis. However, the mechanism underlying ROS-induced anthocyanin accumulation is unclear. In this study, we determined that the HD-Zip I family member transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis under high-light stress in pear (Pyrus ussuriensis). Specifically, PuHB40 induces the PuMYB123-like-PubHLH3 transcription factor complex for anthocyanin biosynthesis. The PuHB40-mediated transcriptional activation depends on its phosphorylation level, which is regulated by protein phosphatase PP2A. Elevated ROS content maintains high PuHB40 phosphorylation levels while also enhancing the PuHB40-induced PuMYB123-like transcription by decreasing the PuPP2AA2 expression, ultimately leading to increased anthocyanin biosynthesis. Our study reveals a pathway regulating the ROS-induced anthocyanin biosynthesis in pears, further clarifying the mechanism underlying the abiotic stress-induced anthocyanin biosynthesis, which may have implications for improving plant stress tolerance.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagosome biogenesis and organelle homeostasis in plant cells. 植物细胞中自噬体的生物生成和细胞器的平衡。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae099

Xiaohong Zhuang, Baiying Li, Liwen Jiang

引用次数: 0

ASYNAPSIS3 has diverse dosage-dependent effects on meiotic crossover formation in Brassica napus. ASYNAPSIS3 对甘蓝型油菜减数分裂交叉形成具有多种剂量依赖性影响。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae207

Lei Chu, Jixin Zhuang, Miaowei Geng, Yashi Zhang, Jing Zhu, Chunyu Zhang, Arp Schnittger, Bin Yi, Chao Yang

{"title":"ASYNAPSIS3 has diverse dosage-dependent effects on meiotic crossover formation in Brassica napus.","authors":"Lei Chu, Jixin Zhuang, Miaowei Geng, Yashi Zhang, Jing Zhu, Chunyu Zhang, Arp Schnittger, Bin Yi, Chao Yang","doi":"10.1093/plcell/koae207","DOIUrl":"10.1093/plcell/koae207","url":null,"abstract":"Crossovers create genetic diversity and are required for equal chromosome segregation during meiosis. Crossover number and distribution are highly regulated by different mechanisms that are not yet fully understood, including crossover interference. The chromosome axis is crucial for crossover formation. Here, we explore the function of the axis protein ASYNAPSIS3. To this end, we use the allotetraploid species Brassica napus; due to its polyploid nature, this system allows a fine-grained dissection of the dosage of meiotic regulators. The simultaneous mutation of all 4 ASY3 alleles results in defective synapsis and drastic reduction of crossovers, which is largely rescued by the presence of only one functional ASY3 allele. Crucially, while the number of class I crossovers in mutants with 2 functional ASY3 alleles is comparable to that in wild type, this number is significantly increased in mutants with only one functional ASY3 allele, indicating that reducing ASY3 dosage increases crossover formation. Moreover, the class I crossovers on each bivalent in mutants with 1 functional ASY3 allele follow a random distribution, indicating compromised crossover interference. These results reveal the distinct dosage-dependent effects of ASY3 on crossover formation and provide insights into the role of the chromosome axis in patterning recombination.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis. 叶绿体内蛋白酶：叶绿体蛋白水解的整体网络观点。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae178

Klaas J van Wijk

引用次数: 0

Transcriptomic response to nitrogen availability reveals signatures of adaptive plasticity during tetraploid wheat domestication. 转录组对氮可用性的响应揭示了四倍体小麦驯化过程中的适应可塑性特征。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae202

Alice Pieri, Romina Beleggia, Tania Gioia, Hao Tong, Valerio Di Vittori, Giulia Frascarelli, Elena Bitocchi, Laura Nanni, Elisa Bellucci, Fabio Fiorani, Nicola Pecchioni, Stefania Marzario, Concetta De Quattro, Antonina Rita Limongi, Pasquale De Vita, Marzia Rossato, Ulrich Schurr, Jacques L David, Zoran Nikoloski, Roberto Papa

{"title":"Transcriptomic response to nitrogen availability reveals signatures of adaptive plasticity during tetraploid wheat domestication.","authors":"Alice Pieri, Romina Beleggia, Tania Gioia, Hao Tong, Valerio Di Vittori, Giulia Frascarelli, Elena Bitocchi, Laura Nanni, Elisa Bellucci, Fabio Fiorani, Nicola Pecchioni, Stefania Marzario, Concetta De Quattro, Antonina Rita Limongi, Pasquale De Vita, Marzia Rossato, Ulrich Schurr, Jacques L David, Zoran Nikoloski, Roberto Papa","doi":"10.1093/plcell/koae202","DOIUrl":"10.1093/plcell/koae202","url":null,"abstract":"The domestication of crops, coupled with agroecosystem development, is associated with major environmental changes and provides an ideal model of phenotypic plasticity. Here, we examined 32 genotypes of three tetraploid wheat (Triticum turgidum L.) subspecies, wild emmer, emmer, and durum wheat, which are representative of the key stages in the domestication of tetraploid wheat. We developed a pipeline that integrates RNA-Seq data and population genomics to assess gene expression plasticity and identify selection signatures under diverse nitrogen availability conditions. Our analysis revealed differing gene expression responses to nitrogen availability across primary (wild emmer to emmer) and secondary (emmer to durum wheat) domestication. Notably, nitrogen triggered the expression of twice as many genes in durum wheat compared to that in emmer and wild emmer. Unique selection signatures were identified at each stage: primary domestication mainly influenced genes related to biotic interactions, whereas secondary domestication affected genes related to amino acid metabolism, in particular lysine. Selection signatures were found in differentially expressed genes (DEGs), notably those associated with nitrogen metabolism, such as the gene encoding glutamate dehydrogenase (GDH). Overall, our study highlights the pivotal role of nitrogen availability in the domestication and adaptive responses of a major food crop, with varying effects across different traits and growth conditions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNE1 scissorhands: How the power of omics sheds light on the control of mRNA decay. DNE1 Scissorhands：全息图学的力量如何揭示 mRNA 的衰变控制。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae219

Margot Raffeiner

引用次数: 0

A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway. 一种货物分拣受体通过分泌途径介导叶绿体蛋白质的运输。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae197

Jinling Liu, Hong Chen, Li Liu, Xiangzhao Meng, Qianwen Liu, Qinyi Ye, Jiangqi Wen, Tao Wang, Jiangli Dong

{"title":"A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway.","authors":"Jinling Liu, Hong Chen, Li Liu, Xiangzhao Meng, Qianwen Liu, Qinyi Ye, Jiangqi Wen, Tao Wang, Jiangli Dong","doi":"10.1093/plcell/koae197","DOIUrl":"10.1093/plcell/koae197","url":null,"abstract":"Nucleus-encoded chloroplast proteins can be transported via the secretory pathway. The molecular mechanisms underlying the trafficking of chloroplast proteins between the intracellular compartments are largely unclear, and a cargo sorting receptor has not previously been identified in the secretory pathway. Here, we report a cargo sorting receptor that is specifically present in Viridiplantae and mediates the transport of cargo proteins to the chloroplast. Using a forward genetic analysis, we identified a gene encoding a transmembrane protein (MtTP930) in barrel medic (Medicago truncatula). Mutation of MtTP930 resulted in impaired chloroplast function and a dwarf phenotype. MtTP930 is highly expressed in the aerial parts of the plant and is localized to the endoplasmic reticulum (ER) exit sites and Golgi. MtTP930 contains typical cargo sorting receptor motifs, interacts with Sar1, Sec12, and Sec24, and participates in coat protein complex II vesicular transport. Importantly, MtTP930 can recognize the cargo proteins plastidial N-glycosylated nucleotide pyrophosphatase/phosphodiesterase (MtNPP) and α-carbonic anhydrase (MtCAH) in the ER and then transport them to the chloroplast via the secretory pathway. Mutation of a homolog of MtTP930 in Arabidopsis (Arabidopsis thaliana) resulted in a similar dwarf phenotype. Furthermore, MtNPP-GFP failed to localize to chloroplasts when transgenically expressed in Attp930 protoplasts, implying that these cargo sorting receptors are conserved in plants. These findings fill a gap in our understanding of the mechanism by which chloroplast proteins are sorted and transported via the secretory pathway.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141535073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant rhabdovirus viroporin P9 facilitates insect-mediated virus transmission in barley. 植物横纹病毒病毒蛋白 P9 可促进昆虫介导的病毒在大麦中的传播。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae162

Qiang Gao, Ying Zang, Ji-Hui Qiao, Zong-Ying Zhang, Ying Wang, Cheng-Gui Han, Xian-Bing Wang

{"title":"The plant rhabdovirus viroporin P9 facilitates insect-mediated virus transmission in barley.","authors":"Qiang Gao, Ying Zang, Ji-Hui Qiao, Zong-Ying Zhang, Ying Wang, Cheng-Gui Han, Xian-Bing Wang","doi":"10.1093/plcell/koae162","DOIUrl":"10.1093/plcell/koae162","url":null,"abstract":"Potassium (K+) plays crucial roles in both plant development and immunity. However, the function of K+ in plant-virus interactions remains largely unknown. Here, we utilized Barley yellow striate mosaic virus (BYSMV), an insect-transmitted plant cytorhabdovirus, to investigate the interplay between viral infection and plant K+ homeostasis. The BYSMV accessory P9 protein exhibits viroporin activity by enhancing membrane permeability in Escherichia coli. Additionally, P9 increases K+ uptake in yeast (Saccharomyces cerevisiae) cells, which is disrupted by a point mutation of glycine 14 to threonine (P9G14T). Furthermore, BYSMV P9 forms oligomers and targets to both the viral envelope and the plant membrane. Based on the recombinant BYSMV-GFP (BYGFP) virus, a P9-deleted mutant (BYGFPΔP9) was rescued and demonstrated infectivity within individual plant cells of Nicotiana benthamiana and insect vectors. However, BYGFPΔP9 failed to infect barley plants after transmission by insect vectors. Furthermore, infection of barley plants was severely impaired for BYGFP-P9G14T lacking P9 K+ channel activity. In vitro assays demonstrate that K+ facilitates virion disassembly and the release of genome RNA for viral mRNA transcription. Altogether, our results show that the K+ channel activity of viroporins is conserved in plant cytorhabdoviruses and plays crucial roles in insect-mediated virus transmission.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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