Plant Cell最新文献_第10页

Repression of ABA-responsive alternative splicing by an Arabidopsis SR protein relieves ABA inhibition of early plant growth. 拟南芥SR蛋白对ABA响应的选择性剪接的抑制减轻了ABA对植物早期生长的抑制。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag127

Tom Laloum, Guiomar Martín, Martin Lewinski, Romana J R Yanez, Tino Köster, Tiago Paixão, Dorothee Staiger, Paula Duque

{"title":"Repression of ABA-responsive alternative splicing by an Arabidopsis SR protein relieves ABA inhibition of early plant growth.","authors":"Tom Laloum, Guiomar Martín, Martin Lewinski, Romana J R Yanez, Tino Köster, Tiago Paixão, Dorothee Staiger, Paula Duque","doi":"10.1093/plcell/koag127","DOIUrl":"https://doi.org/10.1093/plcell/koag127","url":null,"abstract":"The phytohormone abscisic acid (ABA) inhibits postgermination growth under unfavorable conditions to delay the seedling's transition to the autotrophic stage and promote plant survival. Stress-induced ABA accumulation is well established to trigger extensive transcriptional changes, but it is becoming clear that ABA also relies on alternative splicing to enhance stress tolerance. However, the molecular components modulating the posttranscriptional regulation of ABA control of early plant growth remain largely unknown. Here, we show that Arabidopsis thaliana SR34a, a member of the highly conserved serine/arginine-rich (SR) protein family of RNA-binding proteins, reduces sensitivity to ABA, promoting seedling establishment under stress. We found that ABA concentrations that allow greening of wild-type seedlings arrest the growth of a loss-of-function mutant for SR34a upon seed germination. Accordingly, the mutant displays hypersensitivity to drought and salt stress during postgermination growth. Using individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) combined with transcriptome analysis of the mutant, we reveal that SR34a regulates alternative splicing and binds predominantly to GCU-rich exonic sequences near splice sites. SR34a targets all types of alternative splicing events, including in RNAs encoding key ABA pathway regulators, to inhibit ABA-responsive splicing in germinated seeds. Our study establishes SR34a as a splicing regulator, shedding mechanistic light on how SR proteins regulate alternative splicing and counteract ABA inhibition of early plant growth.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 6","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CITF1 fine-tunes FIT activity to regulate Fe uptake. CITF1微调FIT活性以调节铁摄取。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag184

Yuerong Cai, Rihua Lei, Junhui Zhao, Huaqian Ping, Dan Wang, Yang Li, Gang Liang

{"title":"CITF1 fine-tunes FIT activity to regulate Fe uptake.","authors":"Yuerong Cai, Rihua Lei, Junhui Zhao, Huaqian Ping, Dan Wang, Yang Li, Gang Liang","doi":"10.1093/plcell/koag184","DOIUrl":"10.1093/plcell/koag184","url":null,"abstract":"Iron (Fe) is a vital micronutrient essential for plant growth and development. Under Fe-deficient conditions, plants activate their Fe uptake system to ensure adequate Fe supply. The FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT) serves as a central regulator of the Fe uptake system, existing in both inactive and active states in Fe signaling regulation. Plants have evolved intricate mechanisms to modulate FIT activity in order to maintain Fe homeostasis. In this study, we uncover that the CU-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1 (CITF1) regulates Fe uptake by modulating FIT activity. Functional and genetic analyses demonstrate that CITF1 regulates Fe uptake in a FIT-dependent manner. Mechanistic investigations reveal that CITF1 modulates FIT activity through 3 distinct mechanisms: (i) interfering with BTSL1/2 interacting with FIT; (ii) hijacking FIT protein from the nucleus to the cytoplasm; and (iii) maintaining FIT in the FIT-CITF1 dimer to prevent FIT from activating Fe uptake genes. Collectively, our findings suggest that CITF1 regulates Fe uptake by fine-tuning FIT activity, thereby providing novel insights into the regulatory mechanisms of FIT.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving low-phosphate tolerance via tissue-specific CRISPR/Cas9 knockout to balance growth and stress responses in rice. 通过组织特异性CRISPR/Cas9敲除提高水稻低磷酸盐耐受性以平衡生长和胁迫反应

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag170

Guangbo Wei, Zhengwei Huang, Shuman Wang, Fangzhou Zheng, Jiakai Yao, Qiongli Jin, Youchao Zhang, Hongli Luo, Xinlian Zhang, Yichen He, Mingchuan Zhou, Mian Gu, Chuanzao Mao, Zhiye Wang

{"title":"Improving low-phosphate tolerance via tissue-specific CRISPR/Cas9 knockout to balance growth and stress responses in rice.","authors":"Guangbo Wei, Zhengwei Huang, Shuman Wang, Fangzhou Zheng, Jiakai Yao, Qiongli Jin, Youchao Zhang, Hongli Luo, Xinlian Zhang, Yichen He, Mingchuan Zhou, Mian Gu, Chuanzao Mao, Zhiye Wang","doi":"10.1093/plcell/koag170","DOIUrl":"10.1093/plcell/koag170","url":null,"abstract":"Balancing growth and stress responses is critical for improving crop stress tolerance. Inorganic phosphate (Pi) deficiency reduces agricultural yields. Plants have evolved a Pi-starvation response (PSR) network that coordinates growth and responds to fluctuating environmental Pi levels. Null mutations or whole-plant knockdown of PSR repressor genes, such as PHOSPHATE2 (OsPHO2) and OsSPX (Syg1, Pho81, XPR1) family genes, enhance Pi absorption and transfer but disrupt Pi homeostasis, inhibiting growth and reducing yields. To overcome this, we developed a CRISPR/Cas9 tissue-specific knockout (TSKO) system for efficient, vascular-specific somatic knockout of OsPHO2 in rice (Oryza sativa) cv. \"Nipponbare\" across several generations. The plants showed moderately increased Pi concentrations, maintained Pi homeostasis in hydroponic culture, and increased effective tiller number and grain yield in a Pi-deficient paddy. Vascular-specific OsPHO2 knockout moderately increased OsPHO2-repressed, vascular-expressed, Pi-starvation-induced signaling in roots and alleviated disordered PSR in roots and leaves. Vascular-specific knockout of OsPHO2 or OsSPX1/2 in the Zhonghua 11 background gave similar results. Field trials confirmed the enhanced low-Pi tolerance of TSKO plants in a Pi-deficient paddy and these plants showed normal growth in a Pi-sufficient paddy. This highlights the utility of improving rice low-Pi tolerance via a tissue-specific CRISPR/Cas9 knockout, provides insights into the role of vascular tissues in PSR, and offers a promising spatial-targeting strategy for crop improvement.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148205046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal dynamics of ethylene biosynthesis shape infection and nodule initiation in Medicago truncatula. 龙头草乙烯生物合成、形态感染及根瘤形成的时空动态。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag173

Sophia Müller, Thijs Stegmann, Kelvin Adema, Rens Holmer, Amber van Seters, Robin van Velzen, Olga Kulikova, Tristan Wijsman, Joel Klein, Josefina-Patricia Fernandez-Moreno, Anna N Stepanova, Jose M Alonso, Henk Franssen, Estibaliz Larrainzar, Arjan van Zeijl, Wouter Kohlen

{"title":"Spatiotemporal dynamics of ethylene biosynthesis shape infection and nodule initiation in Medicago truncatula.","authors":"Sophia Müller, Thijs Stegmann, Kelvin Adema, Rens Holmer, Amber van Seters, Robin van Velzen, Olga Kulikova, Tristan Wijsman, Joel Klein, Josefina-Patricia Fernandez-Moreno, Anna N Stepanova, Jose M Alonso, Henk Franssen, Estibaliz Larrainzar, Arjan van Zeijl, Wouter Kohlen","doi":"10.1093/plcell/koag173","DOIUrl":"10.1093/plcell/koag173","url":null,"abstract":"Ethylene is a well-established negative regulator of nodulation, yet how ethylene biosynthesis and perception are spatially coordinated during early symbiotic signaling remains unresolved. Here, we investigate the dynamics of ethylene responses in Medicago (Medicago truncatula) using transcriptomics, promoter-reporter analyses, loss-of-function approaches and a synthetic reporter. We show that the activity of the ethylene-responsive EBSn reporter shifts from inner root tissues under nonsymbiotic conditions to the outer cortex and epidermis following rhizobial inoculation, revealing a spatial reprogramming of ethylene signaling. Among the 8 Medicago 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID SYNTHASE (ACS) genes, MtACS3 is induced in outer root cell layers upon rhizobia application, while MtACS10 is repressed in the inner cortex and pericycle, mirroring the shift in ethylene perception. Functional analysis demonstrates that MtACS10 restricts nodule initiation, whereas MtACS3 modulates the number of infection threads, prevents nodule clustering, and contributes to the radial positioning of nodule primordia. Rhizobial induced ectopic ACS expression in the root interior counteracts MtACS10 repression and blocks nodulation, highlighting the requirement for spatially confined downregulation of ethylene biosynthesis. Together, these findings establish a framework in which localized shifts in ethylene biosynthesis, mediated by distinct Medicago ACS genes, balance infection and organogenesis while co-defining the spatial limits of the root susceptible zone.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13291814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal regulation of arbuscular mycorrhizal symbiosis at cellular resolution. 细胞分辨率下丛枝菌根共生的时空调控。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag133

Tania Chancellor, Gabriel Ferreras-Garrucho, Garo Z Akmakjian, Héctor Montero, Sarah Bowden, Matthew S Hope, Emma Wallington, Samik Bhattacharya, Christian Korfhage, Julia Bailey-Serres, Uta Paszkowski

{"title":"Spatiotemporal regulation of arbuscular mycorrhizal symbiosis at cellular resolution.","authors":"Tania Chancellor, Gabriel Ferreras-Garrucho, Garo Z Akmakjian, Héctor Montero, Sarah Bowden, Matthew S Hope, Emma Wallington, Samik Bhattacharya, Christian Korfhage, Julia Bailey-Serres, Uta Paszkowski","doi":"10.1093/plcell/koag133","DOIUrl":"10.1093/plcell/koag133","url":null,"abstract":"Arbuscular mycorrhizal (AM) symbiosis develops through fungal colonization of root epidermal and cortical cells, culminating in the formation of arbuscules, transient, tree-like intracellular hyphal structures for nutrient exchange. To dissect the complexity of AM establishment in rice (Oryza sativa) roots colonized by Rhizophagus irregularis, we conducted spatial transcriptomics of plant and fungal genes at single-cell resolution. This revealed differences in transcriptional activity between fungal structures and reprogramming of plant cell-identity markers upon colonization. Furthermore, cells hosting similarly developed arbuscules showed striking transcriptional heterogeneity, suggesting hidden functional diversity at the individual cell level. For stage-resolved profiling of translation, we used AM-stage specific Translating Ribosome Affinity Purification RNA sequencing (TRAP-seq) with promoters active at discrete stages of symbiosis or arbuscule development. This revealed extensive spatiotemporal changes in the ribosome-bound transcript population, including sets of phosphate, nitrogen, and carbon transporters and regulators with specific enrichment and depletion patterns at different stages of arbuscule development. Rice transcripts encoding cell wall biosynthesis genes and defense markers were present in low abundance at early stages but highly abundant at late stages of the arbuscule lifespan, supporting a host-driven shift toward arbuscule termination. Together, these findings highlight the nuanced dynamic regulation of AM symbiosis at the cellular level, refining our understanding of how nutrient exchange and fungal development are coordinated in space and time.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13237562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin gatekeeping at cooler temperature: VIL1 modulates CBF-driven growth in Arabidopsis. 低温下的染色质守门人：VIL1调节CBF驱动的拟南芥生长。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag044

Crispus M Mbaluto

引用次数: 0

SlWRKY2 orchestrates cold tolerance via phytochromes interaction and SlPIF4 stabilization in tomato. SlWRKY2通过光光色素相互作用和SlPIF4稳定调控番茄的抗寒性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag159

Jiachen Hong, Xiujuan Wang, Xin Liu, Xinjie Sun, Hannah Rae Thomas, Jingquan Yu, Huijia Kang, Yanhong Zhou

{"title":"SlWRKY2 orchestrates cold tolerance via phytochromes interaction and SlPIF4 stabilization in tomato.","authors":"Jiachen Hong, Xiujuan Wang, Xin Liu, Xinjie Sun, Hannah Rae Thomas, Jingquan Yu, Huijia Kang, Yanhong Zhou","doi":"10.1093/plcell/koag159","DOIUrl":"10.1093/plcell/koag159","url":null,"abstract":"Plants orchestrate stress responses through the integration of light and temperature signals, yet the molecular mechanisms involved in this crosstalk are incompletely elucidated. Here, we identify the transcription factor SlWRKY2 as a critical hub linking phytochrome-mediated light perception with cold stress adaptation in tomato (Solanum lycopersicum). Under cold stress, low red/far-red light ratios significantly induce SlWRKY2 expression and protein accumulation, with phytochrome B (SlphyB) repressing and SlphyA promoting this process. Genetic analyses confirm that SlWRKY2 functions downstream of SlphyB and SlphyA, physically interacts with both SlphyB1 and SlphyA, and acts in a regulatory module where SlphyB negatively regulates cold tolerance while SlphyA exerts a positive effect. Moreover, both cold stress and light signals promote the formation of SlWRKY2 condensates consistent with liquid-liquid phase separation. Functionally, SlWRKY2 directly interacts with and stabilizes the light-signaling regulator PHYTOCHROME-INTERACTING TRANSCRIPTION 4 (SlPIF4). Furthermore, SlWRKY2 directly activates SlPIF4 transcription, and the 2 factors function synergistically to enhance the expression of SlCBFs, as evidenced by genetic data indicating that SlPIF4 is required for SlWRKY2-dependent cold tolerance. Mechanistically, SlWRKY2 competes with SlphyB1 for interaction with SlPIF4. Our findings reveal the SlphyB1/A-SlWRKY2-SlPIF4 signaling module as a pivotal mechanism underlying light quality-dependent cold adaptation, advancing our understanding of how plants integrate environmental cues to fine-tune cold adaptation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148157666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural basis of NSP1-NSP2 heterodimerization and its regulatory mechanism in legume nodulation. 豆科植物结瘤中NSP1-NSP2异源二聚化的结构基础及其调控机制

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag161

Lihao Wan, Chunmei He, Chongyu Xue, Huangqi Chen, Xiakun Mao, Yaqi Peng, Xingming Lian, Xuelu Wang, Shutong Xu

{"title":"Structural basis of NSP1-NSP2 heterodimerization and its regulatory mechanism in legume nodulation.","authors":"Lihao Wan, Chunmei He, Chongyu Xue, Huangqi Chen, Xiakun Mao, Yaqi Peng, Xingming Lian, Xuelu Wang, Shutong Xu","doi":"10.1093/plcell/koag161","DOIUrl":"10.1093/plcell/koag161","url":null,"abstract":"Legumes establish symbiotic relationships with rhizobia, leading to the development of nitrogen-fixing root nodules. Two GRAS transcription factors, nodulation signaling pathway (NSP) 1 and NSP2, are essential for Nod factor-induced transcription and subsequent nodulation in legumes. However, the structural basis of their interaction and functional mechanism remains poorly understood. Here, we report the crystal structure of the Medicago truncatula NSP1-NSP2 complex at 2.4 Å resolution. The structure reveals that NSP1 and NSP2 assemble into a heterodimer with a small, triangular interface exclusively composed of their leucine heptad repeat I motifs. This direct interaction is essential for nodulation, as NSP2 facilitates NSP1-DNA binding. Furthermore, we identified an HCCC-type zinc finger in NSP1 that modulates nodulation by influencing its DNA-binding activity. Together, our findings provide structural insights into NSP1-NSP2 heterodimerization and elucidate the regulatory mechanism underlying legume nodulation, offering a theoretical foundation for rationally engineering NSP1 and NSP2 to optimize plant-microbe relationships for agricultural applications.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities. 揭示植物蛋白水解：基因编码荧光传感器作为分析蛋白酶活性的工具。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag162

Álvaro Daniel Fernández-Fernández, Simon Tack, Matthias Van Durme, Eugenia Pitsili, Jingjing Huang, Jonah Nolf, Rosa López García, Robin Pottie, Moritz K Nowack, Jens Staal, Simon Stael, Frank Van Breusegem

{"title":"Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities.","authors":"Álvaro Daniel Fernández-Fernández, Simon Tack, Matthias Van Durme, Eugenia Pitsili, Jingjing Huang, Jonah Nolf, Rosa López García, Robin Pottie, Moritz K Nowack, Jens Staal, Simon Stael, Frank Van Breusegem","doi":"10.1093/plcell/koag162","DOIUrl":"10.1093/plcell/koag162","url":null,"abstract":"Proteolysis is a universal process, as proteases play a pivotal role in modulating numerous signaling pathways. Proteases control the fate and function of their target proteins by hydrolyzing peptide bonds within these proteins. Understanding the temporal and spatial dynamics of proteolytic events, including the proteases that execute them, is crucial for elucidating their particular roles across diverse biological processes. In this study, we developed and characterized a set of genetically encoded Förster resonance energy transfer (FRET)-based reporters for the detection of various proteolytic activities in plants. Our sensors reliably reported the activity of specific proteases, exhibiting a performance comparable to previously established detection systems. In addition, we engineered variants capable of detecting the spatial dynamics of metacaspase-triggered proteolysis after wounding and during programmed cell death in roots. We demonstrated the feasibility of these FRET-based sensors for detecting various activities in vivo with high spatiotemporal resolution. The implementation of these tools in plant research opens opportunities to explore proteolytic mechanisms with enhanced precision. Overall, these biosensors constitute a versatile toolbox for probing protease function within its native cellular context, paving the way for deeper insights into plant biology and signaling.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

StNF-YC9 and StWRKY75 synergistically regulate StPAP10b-mediated root phosphatase activity to drive soil organic phosphorus mobilization in potato. StNF-YC9和StWRKY75协同调节stpap10b介导的根系磷酸酶活性，驱动马铃薯土壤有机磷动员。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag183

Xiaocheng Tian, Peng Zheng, Hanyi Wang, Markus Wirtz, Wenming Zhao, Xiaojia Huang, Cuiyue Liang, Mingjun Li, Shilong Fan, Shaoqun Liu, Zhonghua Liu, Hongbo Zhao

{"title":"StNF-YC9 and StWRKY75 synergistically regulate StPAP10b-mediated root phosphatase activity to drive soil organic phosphorus mobilization in potato.","authors":"Xiaocheng Tian, Peng Zheng, Hanyi Wang, Markus Wirtz, Wenming Zhao, Xiaojia Huang, Cuiyue Liang, Mingjun Li, Shilong Fan, Shaoqun Liu, Zhonghua Liu, Hongbo Zhao","doi":"10.1093/plcell/koag183","DOIUrl":"10.1093/plcell/koag183","url":null,"abstract":"Phosphate limitation represents a major constraint for crop production worldwide. Potato (Solanum tuberosum L.), a globally essential staple crop, is highly susceptible to low inorganic phosphate (LP) stress, yet the underlying molecular responses remain poorly understood. Here, we report that StPAP10b, a purple acid phosphatase gene, is strongly upregulated in roots under LP stress. Among 16 StPAPs analyzed, StPAP10b localizes to the cell wall and exhibits a broad substrate specificity, efficiently hydrolyzing organic phosphorus (Po) sources into bioavailable inorganic phosphorus (Pi). Functional studies confirm that the release of the StPAP10b protein into the rhizosphere enhances root acid phosphatases (APase) activity, facilitating Po utilization under LP. Furthermore, we identify a transcriptional regulatory module in which StWRKY75 directly activates StPAP10b expression by binding to its promoter. This regulation is potentiated by StNF-YC9, which interacts with StWRKY75 and synergistically amplifies StPAP10b transcription. Silencing StPAP10b in StWRKY75-overexpressing roots demonstrated that StWRKY75-mediated LP adaptation partially depends on StPAP10b. Together, these findings reveal the StNF-YC9-StWRKY75-StPAP10b axis as a key regulatory module that enhances extracellular APase activity and Po acquisition, providing strategic insights for improving phosphorus use efficiency in potato.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0