Plant Cell最新文献_第9页

The E3 ligase OsPUB33 controls rice grain size and weight by regulating the OsNAC120-BG1 module. E3连接酶OsPUB33通过调控OsNAC120-BG1模块来控制水稻谷粒的大小和重量。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae297

Zizhao Xie, Ying Sun, Chenghang Zhan, Chengfeng Qu, Ning Jin, Xinyue Gu, Junli Huang

{"title":"The E3 ligase OsPUB33 controls rice grain size and weight by regulating the OsNAC120-BG1 module.","authors":"Zizhao Xie, Ying Sun, Chenghang Zhan, Chengfeng Qu, Ning Jin, Xinyue Gu, Junli Huang","doi":"10.1093/plcell/koae297","DOIUrl":"10.1093/plcell/koae297","url":null,"abstract":"Grain size and weight are important determinants of crop yield. Although the ubiquitin pathway has been implicated in the grain development in rice (Oryza sativa), the underlying genetic and molecular mechanisms remain largely unknown. Here, we report that the plant U-box E3 ubiquitin ligase OsPUB33 interferes with the OsNAC120-BG1 module to control rice grain development. Functional loss of OsPUB33 triggers elevated photosynthetic rates and greater sugar translocation, leading to enhanced cell proliferation and accelerated grain filling. These changes cause enlarged spikelet hulls, thereby increasing final grain size and weight. OsPUB33 interacts with transcription factor OsNAC120, resulting in its ubiquitination and degradation. Unlike OsPUB33, OsNAC120 promotes grain size and weight: OsNAC120-overexpression plants harbor large and heavy grains, whereas osnac120 loss-of-function mutants produce small grains. Genetic interaction analysis supports that OsPUB33 and OsNAC120 function at least partially in a common pathway to control grain development, but have opposite functions. Additionally, OsNAC120 transcriptionally activates BIG GRAIN1 (BG1), a prominent modulator of grain size, whereas OsPUB33 impairs the OsNAC120-mediated regulation of BG1. Collectively, our findings uncover an important molecular framework for the control of grain size and weight by the OsPUB33-OsNAC120-BG1 regulatory module and provide promising targets for improving crop yield.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternally expressed FERTILIZATION-INDEPENDENT ENDOSPERM1 regulates seed dormancy and aleurone development in rice. 母源表达的 OsFERTILIZATION INDEPENDENT ENDOSPERM1 调控水稻的种子休眠和胚乳发育。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae304

Xiaojun Cheng, Su Zhang, Zhiguo E, Zongju Yang, Sijia Cao, Rui Zhang, Baixiao Niu, Qian-Feng Li, Yong Zhou, Xin-Yuan Huang, Qiao-Quan Liu, Chen Chen

{"title":"Maternally expressed FERTILIZATION-INDEPENDENT ENDOSPERM1 regulates seed dormancy and aleurone development in rice.","authors":"Xiaojun Cheng, Su Zhang, Zhiguo E, Zongju Yang, Sijia Cao, Rui Zhang, Baixiao Niu, Qian-Feng Li, Yong Zhou, Xin-Yuan Huang, Qiao-Quan Liu, Chen Chen","doi":"10.1093/plcell/koae304","DOIUrl":"10.1093/plcell/koae304","url":null,"abstract":"Seed dormancy, an essential trait for plant adaptation, is determined by the embryo itself and the surrounding tissues. Here, we found that rice (Oryza sativa) FERTILIZATION-INDEPENDENT ENDOSPERM1 (OsFIE1) regulates endosperm-imposed dormancy and the dorsal aleurone thickness in a manner dependent on the parent of origin. Maternally expressed OsFIE1 suppresses gibberellin (GA) biosynthesis in the endosperm by depositing trimethylation of lysine 27 on histone H3 (H3K27me3) marks on GA biosynthesis-related genes, thus inhibiting germination and aleurone differentiation. Knockout of rice GA 20-oxidase1 (OsGA20ox1) alleviated the phenotypic defects in osfie1. The aleurone-positive determinant Crinkly 4 (OsCR4) is another target of the OsFIE1-containing Polycomb repressive complex 2 (PRC2). We found that OsFIE1 plays an important role in genomic imprinting in the endosperm of germinating seeds, particularly for paternally expressed genes associated with H3K27me3. The increased aleurone thickness of osfie1 substantially improved grain nutritional quality, indicating that the osfie1 gene may be utilized for breeding nutrient-enriched rice. The findings provide insights into the essential roles of PRC2-mediated H3K27me3 methylation in the acquisition of seed dormancy and endosperm cell differentiation in rice.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A natural variant of NON-RIPENING promotes fruit ripening in watermelon. NON-RIPENING 天然变体可促进西瓜果实成熟。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae313

Jinfang Wang, Yongtao Yu, Shaogui Guo, Jie Zhang, Yi Ren, Shouwei Tian, Maoying Li, Shengjin Liao, Guoyi Gong, Haiying Zhang, Yong Xu

{"title":"A natural variant of NON-RIPENING promotes fruit ripening in watermelon.","authors":"Jinfang Wang, Yongtao Yu, Shaogui Guo, Jie Zhang, Yi Ren, Shouwei Tian, Maoying Li, Shengjin Liao, Guoyi Gong, Haiying Zhang, Yong Xu","doi":"10.1093/plcell/koae313","DOIUrl":"10.1093/plcell/koae313","url":null,"abstract":"The regulation of non-climacteric fruit ripening by the transcription factor NON-RIPENING (NOR) is poorly understood. Here, we identified that the NOR homolog in the non-climacteric fruit watermelon (Citrullus lanatus) was located within the selective sweep and sweetness quantitative trait locus that was selected during domestication from landraces to cultivars. ClNOR knockout substantially delayed fruit ripening, and the fruits of the knockout plants had lower abscisic acid (ABA) levels, lighter colored flesh, and were less sweet compared to wild type. Transcriptome analysis and DNA affinity purification sequencing revealed that ClNOR targeted the Basic Leucine Zipper gene ClbZIP1, which links ClNOR to genes that do not have a ClNOR-binding motif in their promoters, such as the ABA biosynthesis gene, 9-cis-epoxycarotenoid dioxygenase ClNCED1 and the chromoplast phosphate transporter gene ClPHT4;2. The double mutant Clnor Clbzip1 exhibited delayed fruit ripening, lower ABA level, and lighter colored flesh. Its delayed ripening phenotype was stronger than that of the Clbzip1 single mutant. Additionally, the ClNORT,T haplotype in cultivated watermelon resulted in higher ClbZIP1 expression, but ClNORC,T from landraces and ClNORC,G from ancestral watermelon did not. Heterologous ClNORT,T expression rescued the delayed ripening phenotype of the Slnor knockout in tomato (Solanum lycopersicum). This natural variant (564T/C) of ClNOR promoted fruit ripening by enhancing target genes transcription. Overall, these findings will help elucidate the evolutionary mechanisms of nonclimacteric fruit ripening.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11702300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142740153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice. 木聚糖o -乙酰转移酶6通过与IRX10形成复合物促进木聚糖合成，并控制水稻细胞壁的形成。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae322

Zhao Wen, Zuopeng Xu, Lanjun Zhang, Yi Xue, Hang Wang, Lin Jian, Jianing Ma, Zhuolin Liu, Hanlei Yang, Shaohui Huang, Xue Kang, Yihua Zhou, Baocai Zhang

{"title":"XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice.","authors":"Zhao Wen, Zuopeng Xu, Lanjun Zhang, Yi Xue, Hang Wang, Lin Jian, Jianing Ma, Zhuolin Liu, Hanlei Yang, Shaohui Huang, Xue Kang, Yihua Zhou, Baocai Zhang","doi":"10.1093/plcell/koae322","DOIUrl":"10.1093/plcell/koae322","url":null,"abstract":"Xylan, a pivotal polymer with diversified structures, is indispensable for cell wall integrity and contributes to plant growth and biomass recalcitrance. Xylan is synthesized by multienzyme complexes named xylan synthase complexes (XSCs). However, the biochemical mechanism of XSCs and the functions of core components within XSC remain unclear. Here, we report that rice (Oryza sativa) XYLAN O-ACETYLTRANSFERASE 6 (XOAT6) and the xylan synthase IRREGULAR XYLEM10 (IRX10) represent core components of the XSC, acting together to biosynthesize acetyl-xylans. Co-fractionation mass spectrometry and protein-protein interaction analyses revealed that IRX10 and XOAT6 physically interact within XSC, corroborated by similar xylan defects in xoat6 and irx10 mutants. Biochemical assays showed that XOAT6 is an O-acetyltransferase of the xylan backbone and facilitates chain polymerization catalyzed by IRX10. Fluorescence correlation spectroscopy further visualized the xylooligomer polymerization process at a single-molecule level. Solid-state NMR analysis, electron microscopy observations, and nanoindentation examinations identified the altered xylan conformation, disorganized cellulosic structure, and increased wall rigidity and cellulose accessibility in the mutants, leading to brittleness and improved saccharification efficiency. Our findings provide insights into the assembly of XSCs and xylan biosynthesis and offer a framework for tailoring xylans to improve crop traits and biomass.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-driven changes in membrane fluidity differentially impact FILAMENTATION TEMPERATURE-SENSITIVE H2-mediated photosystem II repair. 温度驱动的膜流动性变化对成丝温度敏感的h2介导的光系统II修复有不同的影响。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae323

Jingzhi Zhang, Keun Pyo Lee, Yanling Liu, Chanhong Kim

{"title":"Temperature-driven changes in membrane fluidity differentially impact FILAMENTATION TEMPERATURE-SENSITIVE H2-mediated photosystem II repair.","authors":"Jingzhi Zhang, Keun Pyo Lee, Yanling Liu, Chanhong Kim","doi":"10.1093/plcell/koae323","DOIUrl":"10.1093/plcell/koae323","url":null,"abstract":"The Arabidopsis (Arabidopsis thaliana) yellow variegated2 (var2) mutant, lacking functional FILAMENTATION TEMPERATURE-SENSITIVE H2 (FtsH2), an ATP-dependent zinc metalloprotease, is a powerful tool for studying the photosystem II (PSII) repair process in plants. FtsH2, forming hetero-hexamers with FtsH1, FtsH5, and FtsH8, plays an indispensable role in PSII proteostasis. Although abiotic stresses like cold and heat increase chloroplast reactive oxygen species (ROS) and PSII damage, var2 mutants behave like wild-type plants under heat stress but collapse under cold stress. Our study on transgenic var2 lines expressing FtsH2 variants, defective in either substrate extraction or proteolysis, reveals that cold stress causes an increase in membrane viscosity, demanding more substrate extraction power than proteolysis by FtsH2. Overexpression of FtsH2 lacking substrate extraction activity does not rescue the cold-sensitive phenotype, while overexpression of FtsH2 lacking protease activity does in var2, with other FtsH isomers present. This indicates that FtsH2's substrate extraction activity is indispensable under cold stress when membranes become more viscous. As temperatures rise and membrane fluidity increases, substrate extraction activity from other isomers suffices, explaining the var2 mutant's heat stress resilience. These findings underscore the direct effect of membrane fluidity on the functionality of the thylakoid FtsH complex under stress. Future research should explore how membrane fluidity impacts proteostasis, potentially uncovering strategies to modulate thermosensitivity.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11684078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The MaNAP1-MaMADS1 transcription factor module mediates ethylene-regulated peel softening and ripening in banana. 修正：MaNAP1-MaMADS1转录因子模块介导乙烯调控的香蕉果皮软化和成熟。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae319

引用次数: 0

A new light on the UFO mystery: Zmufo1 encodes a nuclear protein that modulates redox levels and epigenetic status during basal endosperm differentiation in maize. UFO之谜的新发现Zmufo1 编码一种核蛋白，它能在玉米基部胚乳分化过程中调节氧化还原水平和表观遗传状态。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae307

Nicolas M Doll

引用次数: 0

From the archives: Roles of microtubule-associated proteins in organelle movement, tip growth, and phragmoplast architecture. 来自档案：微管相关蛋白在细胞器运动、尖端生长和膜质体结构中的作用。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae331

Renuka Kolli

引用次数: 0

EXECUTER1 and singlet oxygen signaling: A reassessment of nuclear activity. EXECUTER1 和单线态氧信号：核活动的重新评估

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae296

Kaiwei Liu, Huan Zhao, Keun Pyo Lee, Qing Yu, Minghui Di, Liangsheng Wang, Chanhong Kim

{"title":"EXECUTER1 and singlet oxygen signaling: A reassessment of nuclear activity.","authors":"Kaiwei Liu, Huan Zhao, Keun Pyo Lee, Qing Yu, Minghui Di, Liangsheng Wang, Chanhong Kim","doi":"10.1093/plcell/koae296","DOIUrl":"10.1093/plcell/koae296","url":null,"abstract":"Chloroplasts are recognized as environmental sensors, capable of translating environmental fluctuations into diverse signals to communicate with the nucleus. Among the reactive oxygen species produced in chloroplasts, singlet oxygen (1O2) has been extensively studied due to its dual roles, encompassing both damage and signaling activities, and the availability of conditional mutants overproducing 1O2 in chloroplasts. In particular, investigating the Arabidopsis (Arabidopsis thaliana) mutant known as fluorescent (flu) has led to the discovery of EXECUTER1 (EX1), a plastid 1O2 sensor residing in the grana margin of the thylakoid membrane. 1O2-triggered EX1 degradation is critical for the induction of 1O2-responsive nuclear genes (SOrNGs). However, a recent study showed that EX1 relocates from chloroplasts to the nucleus upon 1O2 release, where it interacts with WRKY18 and WRKY40 (WRKY18/40) transcription factors to regulate SOrNG expression. In this study, we challenge this assertion. Our confocal microscopy analysis and subcellular fractionation assays demonstrate that EX1 does not accumulate in the nucleus. While EX1 appears in nuclear fractions, subsequent thermolysin treatment assays indicate that it adheres to the outer nuclear region rather than localizing inside the nucleus. Furthermore, luciferase complementation imaging and yeast 2-hybrid assays reveal that EX1 does not interact with nuclear WRKY18/40. Consequently, our study refines the current model of 1O2 signaling by ruling out the nuclear relocation of intact EX1 as a means of communication between the chloroplast and nucleus.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142582762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasmonate induces translation of the Arabidopsis transfer RNA-binding protein YUELAO1, which activates MYC2 in jasmonate signaling. 茉莉酸诱导拟南芥 tRNA 结合蛋白 YUELAO1 翻译，从而激活茉莉酸信号转导中的 MYC2。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae294

Jiahui Wang, Yuanyuan Li, Yanru Hu, Sirui Zhu

{"title":"Jasmonate induces translation of the Arabidopsis transfer RNA-binding protein YUELAO1, which activates MYC2 in jasmonate signaling.","authors":"Jiahui Wang, Yuanyuan Li, Yanru Hu, Sirui Zhu","doi":"10.1093/plcell/koae294","DOIUrl":"10.1093/plcell/koae294","url":null,"abstract":"Jasmonate is ubiquitous in the plant kingdom and regulates multiple physiological processes. Although jasmonate signaling has been thoroughly investigated in Arabidopsis thaliana, most studies have focused on the transcriptional mechanisms underlying various jasmonate responses. It remains unclear whether (and how) translation-related pathways help improve transcription efficiency to modulate jasmonate signaling, which may enable plants to respond to stressful conditions effectively. Here, we demonstrate that jasmonate induces translation of the transfer RNA (tRNA)-binding protein YUELAO 1 (YL1) via a specific region in its 3' untranslated region (3' UTR). YL1 and its homolog YL2 redundantly stimulate jasmonate responses such as anthocyanin accumulation and root growth inhibition, with the YL1 3' UTR being critical for YL1-promoted jasmonate responses. Once translated, YL1 acts as an activator of the MYC2 transcription factor through direct interaction, and disrupting YL1 3' UTR impairs the YL1-mediated transcriptional activation of MYC2. YL1 enhances jasmonate responses mainly in a MYC2-dependent manner. Together, these findings reveal a translational mechanism involved in jasmonate signaling and advance our understanding of the transcriptional regulation of jasmonate signaling. The YL1 3' UTR acts as a crucial signal transducer that integrates translational and transcriptional regulation, allowing plants to respond to jasmonate in a timely fashion.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0