Plant Cell最新文献_第9页

The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense. 环状RNA circANK通过抑制microrna398b介导的防御来抑制水稻对细菌性枯萎病的抗性。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-09 DOI: 10.1093/plcell/koaf082

Xiaohui Liu, Peihong Wang, Sai Wang, Weixue Liao, Mingyan Ouyang, Sisi Lin, Rongpeng Lin, Panagiotis F Sarris, Vasiliki Michalopoulou, Xurui Feng, Zinan Zhang, Zhengyin Xu, Gongyou Chen, Bo Zhu

{"title":"The circular RNA circANK suppresses rice resistance to bacterial blight by inhibiting microRNA398b-mediated defense.","authors":"Xiaohui Liu, Peihong Wang, Sai Wang, Weixue Liao, Mingyan Ouyang, Sisi Lin, Rongpeng Lin, Panagiotis F Sarris, Vasiliki Michalopoulou, Xurui Feng, Zinan Zhang, Zhengyin Xu, Gongyou Chen, Bo Zhu","doi":"10.1093/plcell/koaf082","DOIUrl":"https://doi.org/10.1093/plcell/koaf082","url":null,"abstract":"Circular RNAs (circRNAs) are prevalent in eukaryotic cells and have been linked to disease progressions. Their unique circular structure and stability make them potential biomarkers and therapeutic targets. Compared to animal models, plant circRNA research is still in its infancy. The lack of effective tools to specifically knock down circRNAs without affecting host gene expression has slowed the progress of plant circRNA research. Here, we have developed a CRISPR-Cas13d tool that can specifically knock down circRNAs in plant systems, successfully achieving the targeted knockdown of circRNAs in rice (Oryza sativa). We further focused on Os-circANK (a circRNA derived from Ankyrin repeat-containing protein), a circRNA differentially expressed in rice upon pathogen infection. Physiological and biochemical experiments revealed that Os-circANK functions as a sponge for miR398b, suppressing the cleavage of Cu/Zn-Superoxidase Dismutase (CSD)1/CSD2/Copper Chaperone for Superoxide Dismutase (CCSD)/Superoxidase Dismutase (SODX) through ceRNA (competing endogenous RNA), leading to reduced ROS levels following Xanthomonas oryzae pv. oryzae (Xoo) infection and a negative regulation of rice resistance to bacterial blight. Our findings indicate Os-circANK inhibits rice resistance to bacterial blight via the microRNA398b(miR398b)/CSD/SOD pathway.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate. 重新排列的红苋菜染色体外环状DNA赋予对草甘膦和草铵膦的抗性。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf069

Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson

{"title":"A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.","authors":"Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson","doi":"10.1093/plcell/koaf069","DOIUrl":"10.1093/plcell/koaf069","url":null,"abstract":"Some herbicide-resistant weeds become resistant by generating additional copies of specific loci. For example, amplification of the locus encoding chloroplastic glutamine synthetase (GS2) produces herbicide resistance in the glufosinate-resistant Palmer amaranth (Amaranthus palmeri) accession MSR2. Previously, overamplification of the glyphosate-resistant gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Palmer amaranth was determined to be driven by an extrachromosomal circular DNA (eccDNA). Here, we describe a rearranged eccDNA that confers resistance to both glyphosate and glufosinate ammonium due to the coduplication of the native chromosomal regions that contain the genes that encode for these herbicides target proteins. In addition to EPSPS, the replicon carries 2 GS2 isoforms (GS2.1 and GS2.2) and other genes. MSR2 samples harbored eccDNA carrying only EPSPS coexisting with eccDNAs harboring both EPSPS and GS2. A second glufosinate-resistant Palmer amaranth accession (MSR1) showed distinct GS2.1 and GS2.2 amplification patterns from MSR2, suggesting the existence of diverse replicons in Palmer amaranth. EPSPS copy number was correlated with both GS2 isoforms copy number in MSR2, further supporting the coexistence of these genes in the same replicon. These findings shed light on the complexity of eccDNA formation in plant systems, with the collection and accumulation of extra pieces of DNA.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11985328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embracing the challenges ahead: a new chapter for the plant cell leadership. 迎接未来的挑战：植物细胞领导的新篇章。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf043

Pablo A Manavella

引用次数: 0

The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner. ATM激酶通过磷酸化依赖性物质稳定磷酸酶MKP2延缓拟南芥叶片衰老。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf066

Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li

{"title":"The kinase ATM delays Arabidopsis leaf senescence by stabilizing the phosphatase MKP2 in a phosphorylation-dependent manner.","authors":"Yi Zhang, Shuya Tan, Jin Hee Kim, Jie Cao, Yaning Zhao, Zhenpei Pang, Junjie Liu, Yonglun Lv, Feng Ding, Jeongsik Kim, Hye Ryun Woo, Xinli Xia, Hongwei Guo, Zhonghai Li","doi":"10.1093/plcell/koaf066","DOIUrl":"10.1093/plcell/koaf066","url":null,"abstract":"Arabidopsis thaliana (Arabidopsis) Ataxia Telangiectasia Mutated (ATM) kinase plays a vital role in orchestrating leaf senescence; however, the precise mechanisms remain elusive. Here, our study demonstrates that ATM kinase activity is essential for mitigating age- and reactive oxygen species-induced senescence, as restoration of wild-type ATM reverses premature senescence in the atm mutant, while a kinase-dead ATM variant is ineffective. ATM physically interacts with and phosphorylates Mitogen-Activated Protein Kinase Phosphatase 2 (MKP2) to enhance stability under oxidative stress. Mutations in putative phosphorylation sites S15/154 on MKP2 disrupt its phosphorylation, stability, and senescence-delaying function. Moreover, mutation of mitogen-activated protein kinase 6, a downstream target of MKP2, alleviates the premature senescence phenotype of the atm mutant. Notably, the dual-specificity protein phosphatase 19 (HsDUSP19), a predicted human counter protein of MPK2, interacts with both ATM and HsATM and extends leaf longevity in Arabidopsis when overexpressed. These findings elucidate the molecular mechanisms underlying the role of ATM in leaf senescence and suggest that the ATM-MKP2 module is likely evolutionarily conserved in regulating the aging process across eukaryotes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis. 非传统的TPX2家族蛋白TPXL3调节α极光激酶在拟南芥纺锤体形态发生中的功能。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf065

Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu

{"title":"The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis.","authors":"Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu","doi":"10.1093/plcell/koaf065","DOIUrl":"10.1093/plcell/koaf065","url":null,"abstract":"Spindle assembly in vertebrates requires the Aurora kinase, which is targeted to microtubules and activated by TPX2 (Targeting Protein of XKLP2). In Arabidopsis (Arabidopsis thaliana), TPX2-LIKE 3 (TPXL3), but not the highly conserved TPX2, is essential. To test the hypothesis that TPXL3 regulates the function of α Aurora kinase in spindle assembly, we generated transgenic Arabidopsis lines expressing an artificial microRNA targeting TPXL3 mRNA (amiR-TPXL3). The resulting mutants exhibited growth retardation, which was linked to compromised TPXL3 expression. In the mutant cells, α Aurora was delocalized from spindle microtubules to the cytoplasm, and spindles were assembled without recognizable poles. A functional TPXL3-GFP fusion protein first prominently appeared on the prophase nuclear envelope. Then, TPXL3-GFP localized to spindle microtubules (primarily toward the spindle poles, like γ-tubulin), and finally to the re-forming nuclear envelope during telophase and cytokinesis. However, TPXL3 was absent from phragmoplast microtubules. In addition, we found that the TPXL3 N-terminal Aurora-binding motif, microtubule-binding domain, and importin-binding motif, but not the C-terminal segment, were required for its mitotic function. Expression of truncated TPXL3 variants enhanced the defects in spindle assembly and seedling growth of amiR-TPXL3 plants. Taken together, our findings uncovered the essential function of TPXL3, but not TPX2, in targeting and activating α Aurora kinase for spindle apparatus assembly in Arabidopsis.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content. 基因型环境互作对甘蓝型油菜种子含油量影响的遗传和转录组分析。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf062

Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo

{"title":"Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.","authors":"Xu Han, Xiaowei Wu, Yawen Zhang, Qingqing Tang, Lingju Zeng, Yunhao Liu, Yuyan Xiang, Keqin Hou, Shuai Fang, Weixia Lei, Haojie Li, Shan Tang, Hu Zhao, Yan Peng, Xuan Yao, Tingting Guo, Yuan-Ming Zhang, Liang Guo","doi":"10.1093/plcell/koaf062","DOIUrl":"10.1093/plcell/koaf062","url":null,"abstract":"The molecular basis underlying crop traits is complex, with gene-by-environment interactions (GEIs) affecting phenotypes. However, quantitative trait nucleotide (QTN)-by-environment interactions (QEIs) and GEIs for seed oil content (SOC) in oil crops are rare. Here, we detected 11 environmentally specific and 10 stable additive QTNs and 11 QEIs for SOC in rapeseed (Brassica napus) using genome-wide association studies. Weighted gene co-expression network analysis identified 8 Environmental-Developmental Gene co-expression Modules for which the eigengenes correlated with SOC and the environment explained a large proportion of the variance in gene expression. By incorporating information from the multi-omics dataset, 17 candidate genes and 11 candidate GEIs for SOC were predicted. We mined 1 GEI candidate, LIGHT-DEPENDENT SHORT HYPOCOTYLS5 (LSH5), around the environmentally specific QTN qspOC.A02.1 and QEI qeOC.A02.1 detected by climatic indices as covariates. BnaA02.LSH5 was highly expressed in early seed development, and its expression varied significantly across planting sites, with a trend opposite to light-related climatic indices. The BnaA02.lsh5 and BnaC02.lsh5 double mutants had lower SOC, hypocotyl length, photosynthesis, and carbon- and energy-related metabolites compared with wild type. Moreover, BnaA02.LSH5 transcriptionally directly repressed BnaA02.pMDH2 in fatty acid β-oxidation and photosynthetic electron transport. We propose that BnaLSH5 affects seed oil accumulation in response to light intensity. This study provides a basis for creating high-oil germplasm that is adapted to specific environments.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bending over backwards for soil emergence: KIPK and KIPK-LIKE1 regulate hypocotyl gravitropic growth. 后弯出土：KIPK和KIPK- like1调节下胚轴向地生长。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf073

Gwendolyn K Kirschner

引用次数: 0

Dawning aurora: TPXL3 activates α-Aurora kinase and regulates mitotic spindle morphogenesis. 曙光：TPXL3激活α-极光激酶，调节有丝分裂纺锤体形态发生。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf074

Renuka Kolli

引用次数: 0

A CRY1-HY5-MYB signaling cascade fine-tunes guard cell reactive oxygen species levels and triggers stomatal opening. CRY1-HY5-MYB信号级联微调保护细胞活性氧水平并触发气孔开放。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf064

Yuankai Chang, Mianmian Shi, Xiao Wang, Hui Cheng, Junli Zhang, Hongrui Liu, Huiruo Wu, Xiaobin Ou, Ke Yu, Xuebin Zhang, Brad Day, Chen Miao, Yi Zhao, Kun Jiang

{"title":"A CRY1-HY5-MYB signaling cascade fine-tunes guard cell reactive oxygen species levels and triggers stomatal opening.","authors":"Yuankai Chang, Mianmian Shi, Xiao Wang, Hui Cheng, Junli Zhang, Hongrui Liu, Huiruo Wu, Xiaobin Ou, Ke Yu, Xuebin Zhang, Brad Day, Chen Miao, Yi Zhao, Kun Jiang","doi":"10.1093/plcell/koaf064","DOIUrl":"10.1093/plcell/koaf064","url":null,"abstract":"Stomatal opening facilitates CO2 uptake and causes water loss via transpiration. Compared with the considerable progress made toward understanding phototropin-mediated blue light (BL) signaling in guard cells, the significance of cryptochromes (CRYs) in stomatal opening and their downstream elements remain largely unknown. Here, we show that 3 homologous MYB transcription factor genes, namely MYB11, MYB12, and MYB111, are rapidly transactivated in guard cells during the dark-to-light transition in Arabidopsis (Arabidopsis thaliana). Genetic characterization of myb mutants demonstrates that these proteins specifically mediate light-induced stomatal opening by promoting local flavonol accumulation, thereby controlling reactive oxygen species homeostasis in guard cells. In response to light, activation of the plasma membrane H+-ATPase is inhibited in the myb11 myb12 myb111 triple mutant, compromising transmembrane K+ influx in the mutant guard cells. Furthermore, we demonstrate that MYB11/12/111 expression in guard cells upon illumination is induced by a CRY1-specific signaling cascade involving ELONGATED HYPOCOTYL 5 (HY5), a direct transcriptional activator of these MYBs. Overall, our work reveals a mechanism by which the CRY1-HY5-MYB module facilitates light-induced stomatal opening, providing evidence that flavonoid metabolism in guard cells is crucial for plant stress tolerance.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The UV-B photoreceptor UVR8 interacts with the LOX1 enzyme to promote stomatal closure through the LOX-derived oxylipin pathway. UV-B光感受器UVR8通过lox衍生的氧脂素途径与LOX1酶相互作用，促进气孔关闭。

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf060

Yan Liu, Jue Wang, Xiaotian Liu, Ting Liao, Hui Ren, Liang Liu, Xi Huang

{"title":"The UV-B photoreceptor UVR8 interacts with the LOX1 enzyme to promote stomatal closure through the LOX-derived oxylipin pathway.","authors":"Yan Liu, Jue Wang, Xiaotian Liu, Ting Liao, Hui Ren, Liang Liu, Xi Huang","doi":"10.1093/plcell/koaf060","DOIUrl":"10.1093/plcell/koaf060","url":null,"abstract":"Ultraviolet-B (UV-B) light-induced stomatal closure requires the photoreceptor UV RESISTANCE LOCUS 8 (UVR8) and nitric oxide (NO). However, the signaling pathways by which UV-B light regulates stomatal closure remain elusive. Here, we reveal that UVR8 signaling in the epidermis mediates stomatal closure in a tissue-specific manner in Arabidopsis (Arabidopsis thaliana). UV-B light promotes PHOSPHOLIPASE 1 (PLIP1)/PLIP3-mediated linoleic acid and α-linolenic acid accumulation and induces LIPOXYGENASE 1 (LOX1) expression. LOX1, which catabolizes linoleic acid and α-linolenic acid to produce oxylipin derivatives, acts downstream of UVR8 and upstream of the salicylic acid (SA) pathway associated with stomatal defense. Photoactivated UVR8 interacts with LOX1 and enhances its activity. Protein crystallography demonstrates that A. thaliana LOX1 and its ortholog in soybean (Glycine max) share overall structural similarity and conserved residues in the oxygen cavity, substrate cavity, and metal-binding site that are required for 9-LOX activity. The disruption of UVR8-LOX1 contact sites near the LOX1 oxygen and substrate cavities prevents UVR8-enhanced LOX1 activity and compromises stomatal closure upon UV-B exposure. Overall, our study uncovers a noncanonical UV-B signaling module, consisting of the UVR8 photoreceptor and the cytoplasmic lipoxygenase, that mediates stomatal responses to UV-B light.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0