Plant Cell最新文献_第9页

Lack of GDSL motif-containing proteins increases drought tolerance by altering the stomatal cuticle in Arabidopsis. 缺乏含有GDSL基序的蛋白通过改变拟南芥的气孔角质层来增加耐旱性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag150

Khushbu Kumari, Ritwika Kar, Viktoria Zeisler-Diehl, Jana Leide, Kerstin Kalkreuter, Minou Nowrousian, Klaus Harter, Lukas Schreiber, Arun Sampathkumar, Magdiel S S Lim, Lea Hembach, Till Ischebeck, Alexis Paucelle, Sandra Richter, Christopher Grefen

{"title":"Lack of GDSL motif-containing proteins increases drought tolerance by altering the stomatal cuticle in Arabidopsis.","authors":"Khushbu Kumari, Ritwika Kar, Viktoria Zeisler-Diehl, Jana Leide, Kerstin Kalkreuter, Minou Nowrousian, Klaus Harter, Lukas Schreiber, Arun Sampathkumar, Magdiel S S Lim, Lea Hembach, Till Ischebeck, Alexis Paucelle, Sandra Richter, Christopher Grefen","doi":"10.1093/plcell/koag150","DOIUrl":"10.1093/plcell/koag150","url":null,"abstract":"Stomata facilitate gas exchange across the otherwise virtually impermeable cuticle of the plant surface. To control this gas exchange, guard cells undergo reversible changes in their shape and volume to regulate the stomatal aperture. The ability of guard cells to undergo these changes likely requires flexibility of the guard cell wall. Here, we functionally characterized GELP80, a meristemoid-expressed Gly-Asp-Ser-Leu (GDSL) lipase that is a target of SPEECHLESS, a key transcription factor regulating stomatal development. Double T-DNA insertion mutants of GELP80 and its closest homolog GELP100 exhibit aberrant stomatal pore morphology. These defects were associated with a significant reduction in stomatal aperture index independent of abscisic acid, decreased transpiration, reduced cuticle permeability, and enhanced drought tolerance. In addition, alterations in wax and cutin composition were detected at the whole-rosette level, based on analyses of entire leaves rather than guard cells. The gelp80 gelp100 guard cells have an altered wax and cell wall composition. GELP80 acts as fatty acyl-ester hydrolase leading to increased degradation of very-long-chain fatty acid (VLCF-C22:0 and C30 acyl-CoA) to free fatty acids (FAs; C22, C30). We hypothesize that these alterations decrease the flexibility of the stomata cell walls, thus impairing guard cell function.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148034141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photocopying the genetic lottery: a 2-gene shortcut to clonal seeds in rapeseed. 复制遗传彩票：油菜克隆种子的双基因捷径。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag174

Yu-Hung Hung

引用次数: 0

Correction to: Multiomics analysis reveals the molecular mechanisms underlying virulence in Rhizoctonia and jasmonic acid-mediated resistance in Tartary buckwheat (Fagopyrum tataricum). 更正：多组学分析揭示了苦荞麦（Fagopyrum tataricum）根核菌毒力和茉莉酸介导的抗性的分子机制。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag171

引用次数: 0

Dynamic epigenetic and transcriptional regulatory network in pepper fruit development and ripening. 辣椒果实发育和成熟的动态表观遗传和转录调控网络。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag211

Qian Liu, Javier Jingheng Tan, Rui Yang, Janelle Shi Min Sim, Xiaoyi Li, Xinran Zhang, Danhua Jiang

{"title":"Dynamic epigenetic and transcriptional regulatory network in pepper fruit development and ripening.","authors":"Qian Liu, Javier Jingheng Tan, Rui Yang, Janelle Shi Min Sim, Xiaoyi Li, Xinran Zhang, Danhua Jiang","doi":"10.1093/plcell/koag211","DOIUrl":"10.1093/plcell/koag211","url":null,"abstract":"Pepper (Capsicum) is among the most widely cultivated and consumed vegetable crops worldwide. Although studies in model fruit crops such as tomato have provided insights into the genetic and epigenetic regulation of fruit development and ripening, comparable knowledge in pepper remains limited. Here, we employ a multiomics approach to investigate transcriptome and epigenome dynamics during pepper fruit pericarp development and ripening. Our analyses reveal coordinated changes in epigenetic modifications accompanying transcriptome reprogramming. Changes in H3K27ac are closely associated with chromatin accessibility dynamics, and H3K27me3 dynamics may contribute to the transition from fruit growth to ripening. Construction of a transcriptional regulatory network suggests that several ripening regulators identified in climacteric tomato may also play critical roles in governing ripening in nonclimacteric pepper. During ripening, DNA methylation, particularly in the CG and CHG contexts, undergoes global demethylation, especially at promoter regions, accompanied by increased chromatin accessibility and likely enhances transcription factor binding. Such global demethylation resembles that observed in tomato, suggesting a potentially conserved regulatory mechanism during fruit development within the Solanaceae family. Furthermore, comparative analyses between pepper and tomato reveal conserved epigenetic regulation of key ripening-associated transcription factors, alongside distinct regulation of ethylene biosynthesis. We also demonstrate transcriptional and epigenetic regulation of carotenoid and vitamin C biosynthesis pathways in pepper. Collectively, this study provides a comprehensive resource for mechanistic dissection and comparative analysis of pepper fruit development and ripening, with potential applications for crop improvement. To facilitate data exploration, the datasets have been integrated into an online browser at http://pepperfruitatlas.org.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baiting blast fungi: an engineered NLR domain improves effector recognition. 诱饵爆炸真菌：一个工程NLR结构域提高效应识别。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag189

Jan W Huebbers

引用次数: 0

A viral protein-driven ubiquitination switch fine-tunes chloroplast outer envelope protein homeostasis. 病毒蛋白驱动的泛素化开关微调叶绿体外包膜蛋白稳态。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag179

Yanlin Chen, Guanhua Yan, Xinchen Wang, Haorui Liu, Wenli Li, Qinglin Pi, Xiaofei Zhao, Liangsheng Wang, Yongliang Zhang, Xian-Bing Wang, Dawei Li, Meng Yang

{"title":"A viral protein-driven ubiquitination switch fine-tunes chloroplast outer envelope protein homeostasis.","authors":"Yanlin Chen, Guanhua Yan, Xinchen Wang, Haorui Liu, Wenli Li, Qinglin Pi, Xiaofei Zhao, Liangsheng Wang, Yongliang Zhang, Xian-Bing Wang, Dawei Li, Meng Yang","doi":"10.1093/plcell/koag179","DOIUrl":"10.1093/plcell/koag179","url":null,"abstract":"Chloroplast integrity is essential for plant growth, development, and stress resilience; however, viral infection frequently induces chloroplast dysfunction. The mechanism by which viruses perturb and subsequently reprogram chloroplast homeostasis remains poorly understood. Here, we demonstrate that host pheophytinase (PPH) and the barley stripe mosaic virus (BSMV) γb protein engage in an antagonistic interaction to govern the stability of the chloroplast outer-envelope translocon protein Toc34 in Nicotiana benthamiana. Outer envelope-localized PPH recruits the E3 ubiquitin ligase PUB4 to Toc34, driving ubiquitin-dependent degradation of Toc34 and thereby compromising envelope integrity and conferring broad-spectrum antiviral defenses. Upon infection, the viral γb protein directly interacts with PPH and redirects PUB4-mediated ubiquitination toward PPH itself, accelerating PPH turnover and restoring Toc34 levels. This γb-mediated ubiquitination switch preserves envelope integrity and creates a cellular environment that favors viral replication. Our findings reveal a dynamic tripartite PPH-PUB4-γb regulatory circuit that fine-tunes chloroplast homeostasis during infection, illustrating how viruses exploit host ubiquitin machinery to reconcile organelle stability with pathogen fitness.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148227047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compartmental control of UDP-Araf supply: Golgi lumen UDP-Arabinopyranose mutase depletes plant cell wall Araf linkages. UDP-Araf供应的区隔控制：高尔基腔内的udp -阿拉伯吡喃糖突变酶耗尽植物细胞壁Araf键。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag154

Asier Largo-Gosens, Juan P Parra-Rojas, Dayan Sanhueza, Ricardo Yusta, Rachel A Mertz, Christopher K Dugard, Nicholas C Carpita, Susana Saez-Aguayo, Ariel Orellana

{"title":"Compartmental control of UDP-Araf supply: Golgi lumen UDP-Arabinopyranose mutase depletes plant cell wall Araf linkages.","authors":"Asier Largo-Gosens, Juan P Parra-Rojas, Dayan Sanhueza, Ricardo Yusta, Rachel A Mertz, Christopher K Dugard, Nicholas C Carpita, Susana Saez-Aguayo, Ariel Orellana","doi":"10.1093/plcell/koag154","DOIUrl":"10.1093/plcell/koag154","url":null,"abstract":"L-Arabinofuranose (Araf) is a major constituent of plant cell wall polysaccharides. UDP-Araf is generated from UDP-L-arabinopyranose (UDP-Arap) by a UDP-Arap mutase (UAM) on the cytosolic face of the Golgi and then imported into the Golgi lumen for utilization by arabinosyltransferases (AraTs). Yet, a substantial fraction of UDP-Arap is synthesized in the Golgi lumen by the UDP-xylose 4-epimerase (UXE), creating a topological paradox where luminal UDP-Arap must be exported to the cytosol for conversion to UDP-Araf by UAM and then re-imported into the Golgi lumen. To test the functional significance of this compartmentation, we mislocalized Arabidopsis (Arabidopsis thaliana) UAM1 to the Golgi lumen by fusing it to the UXE1 transmembrane domain. The fusion protein was present in the Golgi, and protease-protection assays on microsomes supported its luminal orientation. In wild-type and uam1 plants, Golgi-targeted UAM1 reduced total cell wall Arabinose without consistent changes in other monosaccharides. Linkage analyses showed that this decrease reflects selective loss of Araf residues, whereas Arap remain largely unchanged, consistent with reduced UDP-Araf availability for luminal AraTs. Under salt stress, expression of Golgi-targeted UAM1 in uam1 caused root-growth inhibition and maturation-zone swelling that exogenous L-Ara failed to rescue. These results support a hypothesis that the strong thermodynamic bias of the UAM reaction toward UDP-Arap requires spatial separation of UDP-Arap production from UDP-Araf formation to sustain UDP-Araf flux into Golgi arabinosylation and avoid futile intraluminal back-conversion.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148056242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution. 在单细胞分辨率下，野生草短柄草发育中的叶子。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag172

Lea S Berg, Paola Ruiz Duarte, Inés Hidalgo Prados, Nathan T Lacombe, Rashmi Tandon, Isaia Vardanega, Jan E Maika, Roxane P Spiegelhalder, Ambuj Gore, Heike Lindner, Rüdiger Simon, Michael T Raissig

{"title":"The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution.","authors":"Lea S Berg, Paola Ruiz Duarte, Inés Hidalgo Prados, Nathan T Lacombe, Rashmi Tandon, Isaia Vardanega, Jan E Maika, Roxane P Spiegelhalder, Ambuj Gore, Heike Lindner, Rüdiger Simon, Michael T Raissig","doi":"10.1093/plcell/koag172","DOIUrl":"10.1093/plcell/koag172","url":null,"abstract":"Leaves are the plant's main photosynthetic organs and drive Earth's primary production. Grasses form longitudinal leaves with parallel venation and graminoid stomata. Yet, how distinct leaf tissues coordinately develop to build functional grass leaf anatomy is not well understood. Here, we decoded the developing grass leaf from vegetative meristems to mature tissues using single-cell RNA-sequencing in the wild grass Brachypodium distachyon. In-depth analysis of epidermal clusters and multiplexed whole-mount RNA-fluorescence in situ hybridization resolved most epidermal lineages and confirmed them in planta. Gene regulatory network analysis distinguished the targetomes of the 2 co-expressed, yet functionally divergent stomatal transcription factors BdMUTE and BdFAMA. Finally, we used our dataset to identify and functionally describe the role of the transcription factor gene BdGRAS32 in inhibiting cell division and a stomata-specific role for the putative cell wall-modifying enzyme BdPME53-like. Our single-cell grass leaf atlas enables the dissection of developmental processes that make the grass leaf, which sustains global food production.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13291820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148219402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Teamwork makes the dream work: IPA1 and OsNPR1 work together to enhance immunity in rice. 团队合作让梦想成真：IPA1和OsNPR1共同作用，增强水稻的免疫力。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag153

Annabelle Campbell

引用次数: 0

Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance. 设计大麦NLR的Exo70集成结构域以提高抗稻瘟病能力。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-06-02 DOI: 10.1093/plcell/koag168

Indira Saado, Helen J Brabham, Josh W Bennett, Anson Ho Ching Lam, Inmaculada Hernández-Pinzón, Matthew J Moscou, Juan Carlos De la Concepcion, Mark J Banfield

{"title":"Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.","authors":"Indira Saado, Helen J Brabham, Josh W Bennett, Anson Ho Ching Lam, Inmaculada Hernández-Pinzón, Matthew J Moscou, Juan Carlos De la Concepcion, Mark J Banfield","doi":"10.1093/plcell/koag168","DOIUrl":"10.1093/plcell/koag168","url":null,"abstract":"Intracellular immune receptors protect plants from microbial invasion by detecting and responding to pathogen-derived effector molecules, often triggering cell death responses. However, pathogen effectors can evolve to avoid immune recognition, resulting in devastating diseases that threaten global agriculture. Here, we show that an integrated Exo70 domain from the barley intracellular nucleotide-binding leucine-rich repeat (NLR) RGH2 can interact with both the rice blast pathogen effector AVR-Pii and a closely related wheat blast causing variant. We used structure-led engineering to develop RGH2+ that shows increased binding affinity toward AVR-Pii variants and increased cell death responses on heterologous expression in Nicotiana benthamiana. Infection assays in transgenic barley lines carrying RGH2+ with the paired NLR RGH3 indicate a reduced susceptibility to blast strains expressing AVR-Pii variants. These results demonstrate the potential of engineering NLR receptors as an effective strategy for improving resistance toward one of the most destructive diseases affecting cereal production.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13291817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148212442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0