Plant Cell最新文献_第2页

Living off the fat of the plant: Powdery mildew fungi thrive on host thylakoid lipids.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf044

Jan Wilhelm Hübbers

引用次数: 0

Flipping the sex switch: Genetic insights into sex determination factor in Ceratopteris richardii.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf079

Sonhita Chakraborty

引用次数: 0

How UV-B-activated UVR8 triggers LOX1 to close stomata: Unveiling a mechanism of photoreceptor-regulated enzyme activity in the cytoplasm.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf076

Jiajun Wang

引用次数: 0

Seeing is believing: Whole-cell electron tomography models of vacuole morphology and formation in the early-stage root cortex of Arabidopsis.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf057

Yong Cui, Jiayang Gao, Yanbin Li, Hai Zhang, Xiaohui Zheng, Qing Qi, Shengqi Zhang, Byung-Ho Kang, Liwen Jiang

引用次数: 0

Like Alice in Wonderland, ROOT-ExM enlarges root tips for a closer look.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf078

Laura Arribas-Hernández

引用次数: 0

LTD coordinates chlorophyll biosynthesis and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN transport.

IF 1 1区生物学

Plant Cell Pub Date : 2025-04-02 DOI: 10.1093/plcell/koaf068

Liwei Rong, Junhang An, Xinyue Chen, Chao Wang, Jianghao Wu, Peng Wang, Yongxing Zheng, Xin Wang, Xin Chai, Wei Li, Zhubing Hu, Dandan Lu, Guangyu E Chen, Min Ouyang, Bernhard Grimm, Lixin Zhang, Xiumei Xu

{"title":"LTD coordinates chlorophyll biosynthesis and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN transport.","authors":"Liwei Rong, Junhang An, Xinyue Chen, Chao Wang, Jianghao Wu, Peng Wang, Yongxing Zheng, Xin Wang, Xin Chai, Wei Li, Zhubing Hu, Dandan Lu, Guangyu E Chen, Min Ouyang, Bernhard Grimm, Lixin Zhang, Xiumei Xu","doi":"10.1093/plcell/koaf068","DOIUrl":"10.1093/plcell/koaf068","url":null,"abstract":"Chlorophyll biosynthesis must be tightly coupled to light-harvesting chlorophyll a/b-binding protein (LHCP) biogenesis, as free chlorophyll and its precursors are phototoxic. However, precisely how these 2 processes are coordinated in Arabidopsis (Arabidopsis thaliana) remains elusive. Our previous studies demonstrated the role of LHCP TRANSLOCATION DEFECT (LTD) in delivering LHCPs to the chloroplast via the signal recognition particle-dependent pathway. Here, we show that LTD interacts with and stabilizes the chlorophyll biosynthesis enzymes Mg-protoporphyrin methyltransferase and Mg-protoporphyrin monomethylester (MgPME) cyclase, maintaining their activity. We also demonstrate the direct binding of LTD to MgPME, and through crystal structure analysis, we show that the groove of the LTD dimer is critical for MgPME binding. Thus, we propose that LTD transfers MgPME from Mg-protoporphyrin methyltransferase to the MgPME cyclase. These results elucidate a role for LTD in synchronizing chlorophyll biosynthesis with LHCP transport to ensure the correct insertion of chlorophylls into LHCPs.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.

IF 1 1区生物学

Plant Cell Pub Date : 2025-03-27 DOI: 10.1093/plcell/koaf069

Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson

{"title":"A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.","authors":"Pâmela Carvalho-Moore, Ednaldo A Borgato, Luan Cutti, Aimone Porri, Ingo Meiners, Jens Lerchl, Jason K Norsworthy, Eric L Patterson","doi":"10.1093/plcell/koaf069","DOIUrl":"https://doi.org/10.1093/plcell/koaf069","url":null,"abstract":"Some herbicide-resistant weeds become resistant by generating additional copies of specific loci. For example, amplification of the locus encoding chloroplastic glutamine synthetase (GS2) produces herbicide resistance in the glufosinate-resistant Palmer amaranth (Amaranthus palmeri) accession MSR2. Previously, overamplification of the glyphosate resistance gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Palmer amaranth was determined to be driven by an extrachromosomal circular DNA (eccDNA). Here, we describe a rearranged eccDNA that confers resistance to both glyphosate and glufosinate-ammonium due to the co-duplication of the native chromosomal regions that contain the genes that encode for these herbicides target proteins. In addition to EPSPS, the replicon carries two GS2 isoforms (GS2.1 and GS2.2) and other genes. MSR2 samples harbored eccDNA carrying only EPSPS co-existing with eccDNAs harboring both EPSPS and GS2. A second glufosinate-resistant Palmer amaranth accession (MSR1) showed distinct GS2.1 and GS2.2 amplification patterns from MSR2, suggesting the existence of diverse replicons in Palmer amaranth. EPSPS copy number was correlated with both GS2 isoforms copy number in MSR2, further supporting the co-existence of these genes in the same replicon. These findings shed light on the complexity of eccDNA formation in plant systems, with the collection and accumulation of extra pieces of DNA.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The G protein β subunit SlGB1 regulates tyramine-derived phenolamide metabolism for shoot apex growth and development in tomato.

IF 1 1区生物学

Plant Cell Pub Date : 2025-03-27 DOI: 10.1093/plcell/koaf070

Jiao Wang, Qian Luo, Jingjing Deng, Xiao Liang, Yimei Li, Anran Wang, Teng Lin, Hua Liu, Xuanbo Zhang, Zhaoyu Liu, Zhangjian Hu, Shuting Ding, Changtian Pan, Jingquan Yu, Qifei Gao, Christine H Foyer, Kai Shi

{"title":"The G protein β subunit SlGB1 regulates tyramine-derived phenolamide metabolism for shoot apex growth and development in tomato.","authors":"Jiao Wang, Qian Luo, Jingjing Deng, Xiao Liang, Yimei Li, Anran Wang, Teng Lin, Hua Liu, Xuanbo Zhang, Zhaoyu Liu, Zhangjian Hu, Shuting Ding, Changtian Pan, Jingquan Yu, Qifei Gao, Christine H Foyer, Kai Shi","doi":"10.1093/plcell/koaf070","DOIUrl":"https://doi.org/10.1093/plcell/koaf070","url":null,"abstract":"The shoot apex is a critical determinant of plant growth, development, morphology, and yield. The G protein β subunit (Gβ) is an essential regulator of apical meristem dynamics, yet its precise mechanism of action remains unclear, with notable interspecific variation. This study reveals that in the dicot tomato (Solanum lycopersicum), Gβ subunit mutants (Slgb1) display abnormal shoot morphogenesis and, in severe cases, shoot apex death. Such a phenotype has also been observed in monocot species like maize (Zea mays) and rice (Oryza sativa), but not in the model dicot Arabidopsis (Arabidopsis thaliana). Using integrated multi-omics and liquid chromatography-mass spectrometry, we identified a significant upregulation in tyramine-derived phenolamides in Slgb1 mutants, particularly N-p-trans-coumaroyltyramine (N-P-CT) and N-trans-feruloyltyramine (N-FT). Biochemical and genetic assays pinpointed tyramine hydroxycinnamoyl transferases (THTs) as the enzymes catalyzing N-P-CT and N-FT biosynthesis, with THT8 overexpression inducing shoot apex death. Comparative genomic analysis revealed the presence of a THT-mediated tyramine-derived phenolamide metabolic pathway in species exhibiting gb1 mutant-associated apex death, which is notably absent in Arabidopsis. Protein interaction assays showed that SlGB1 interacts with bHLH79 at the cell membrane and cytoplasm, thereby attenuating the bHLH79-MYB10 interaction within the nucleus, leading to altered THT expression and phenolamide biosynthesis. This study unravels the molecular mechanisms by which SlGB1 governs tomato shoot apex growth and development, highlighting interspecific differences critical for developing breeding strategies aimed at optimizing shoot apex architecture.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143731311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis.

IF 1 1区生物学

Plant Cell Pub Date : 2025-03-26 DOI: 10.1093/plcell/koaf065

Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu

{"title":"The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis.","authors":"Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu","doi":"10.1093/plcell/koaf065","DOIUrl":"https://doi.org/10.1093/plcell/koaf065","url":null,"abstract":"Spindle assembly in vertebrates requires the Aurora kinase, which is targeted to microtubules and activated by TPX2 (Targeting Protein of XKLP2). In Arabidopsis (Arabidopsis thaliana), TPX2-LIKE 3 (TPXL3), but not the highly conserved TPX2, is essential. To test the hypothesis that TPXL3 regulates the function of α Aurora kinase in spindle assembly, we generated transgenic Arabidopsis lines expressing an artificial microRNA targeting TPXL3 mRNA (amiR-TPXL3). The resulting mutants exhibited growth retardation, which was linked to compromised TPXL3 expression. In the mutant cells, α Aurora was delocalized from spindle microtubules to the cytoplasm, and spindles were assembled without recognizable poles. A functional TPXL3-GFP fusion protein first prominently appeared on the prophase nuclear envelope. Then, TPXL3-GFP localized to spindle microtubules (primarily towards the spindle poles, like γ-tubulin), and finally to the re-forming nuclear envelope during telophase and cytokinesis. However, TPXL3 was absent from phragmoplast microtubules. In addition, we found that the TPXL3 N-terminal Aurora-binding motif, microtubule-binding domain, and importin-binding motif, but not the C-terminal segment, were required for its mitotic function. Expression of truncated TPXL3 variants enhanced the defects in spindle assembly and seedling growth of amiR-TPXL3 plants. Taken together, our findings uncovered the essential function of TPXL3, but not TPX2, in targeting and activating α Aurora kinase for spindle apparatus assembly in Arabidopsis.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deficient CP24 allele defines variation for dynamic non-photochemical quenching and photosystem II efficiency in maize.

IF 1 1区生物学

Plant Cell Pub Date : 2025-03-25 DOI: 10.1093/plcell/koaf063

John N Ferguson, Leonardo Caproni, Julia Walter, Katie Shaw, Lucia Arce-Cubas, Alice Baines, Min Soe Thein, Svenja Mager, Georgia Taylor, Lee Cackett, Jyotirmaya Mathan, Richard L Vath, Leo Martin, Bernard Genty, Mario Enrico Pè, Tracy Lawson, Matteo Dell'Acqua, Johannes Kromdijk

{"title":"A deficient CP24 allele defines variation for dynamic non-photochemical quenching and photosystem II efficiency in maize.","authors":"John N Ferguson, Leonardo Caproni, Julia Walter, Katie Shaw, Lucia Arce-Cubas, Alice Baines, Min Soe Thein, Svenja Mager, Georgia Taylor, Lee Cackett, Jyotirmaya Mathan, Richard L Vath, Leo Martin, Bernard Genty, Mario Enrico Pè, Tracy Lawson, Matteo Dell'Acqua, Johannes Kromdijk","doi":"10.1093/plcell/koaf063","DOIUrl":"https://doi.org/10.1093/plcell/koaf063","url":null,"abstract":"Maize (Zea mays L.) is a global crop species in which CO2 assimilation occurs via the C4 pathway. C4 photosynthesis is typically more efficient than C3 photosynthesis under warm and dry conditions; however, despite this inherent advantage, considerable variation remains in photosynthetic efficiency for C4 species that could be leveraged to benefit crop performance. Here, we investigate the genetic architecture of NPQ and photosystem II (PSII) efficiency using a combination of high-throughput phenotyping and quantitative trait loci (QTL) mapping in a field-grown Multi-parent Advanced Generation Inter-Cross (MAGIC) mapping population. QTL mapping was followed by identification of putative candidate genes using a combination of genomics, transcriptomics, protein biochemistry, and targeted physiological phenotyping. We identified four genes with a putative causal role in the observed QTL effects. The highest confidence causal gene was found for a large effect QTL for photosynthetic efficiency on chromosome 10, which was underpinned by allelic variation in the expression of the minor PSII antenna protein light harvesting complex photosystem II subunit (LHCB6 or CP24), mainly driven by poor expression associated with the haplotype of the F7 founder line. The historical role of this line in breeding for early flowering time may suggest that the presence of this deficient allele could be enriched in temperate maize germplasm. These findings advance our understanding of the genetic basis of NPQ and PSII efficiency in C4 plants and highlight the potential for breeding strategies aimed at optimizing photosynthetic efficiency in maize.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0