Plant Cell最新文献_第2页

The Plant Cell welcomes 2025 Assistant Features Editors. 植物细胞欢迎2025助理功能编辑。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koaf001

Nancy A Eckardt, Blake C Meyers, Pablo A Manavella

引用次数: 0

You can have it all: How the interplay between SnRK1 and RBOH1 promotes nitrate uptake in tomato. 你可以拥有一切：SnRK1和RBOH1之间的相互作用如何促进番茄的硝酸盐吸收。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae325

Margot Raffeiner

引用次数: 0

Noncanonical transcription initiation is primarily tissue specific and epigenetically tuned in paleopolyploid plants. 在古多倍体植物中，非规范转录起始主要具有组织特异性，并受到表观遗传学的调整。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae288

Xutong Wang, Jingbo Duan, Chancelor B Clark, Wanjie Feng, Jianxin Ma

{"title":"Noncanonical transcription initiation is primarily tissue specific and epigenetically tuned in paleopolyploid plants.","authors":"Xutong Wang, Jingbo Duan, Chancelor B Clark, Wanjie Feng, Jianxin Ma","doi":"10.1093/plcell/koae288","DOIUrl":"10.1093/plcell/koae288","url":null,"abstract":"Alternative transcription initiation (ATI) appears to be a ubiquitous regulatory mechanism of gene expression in eukaryotes. However, the extent to which it affects the products of gene expression and how it evolves and is regulated remain unknown. Here, we report genome-wide identification and analysis of transcription start sites (TSSs) in various soybean (Glycine max) tissues using a survey of transcription initiation at promoter elements with high-throughput sequencing (STRIPE-seq). We defined 193,579 TSS clusters/regions (TSRs) in 37,911 annotated genes, with 56.5% located in canonical regulatory regions and 43.5% from start codons to 3' untranslated regions, which were responsible for changes in open reading frames of 24,131 genes. Strikingly, 6,845 genes underwent ATI within coding sequences (CDSs). These CDS-TSRs were tissue-specific, did not have TATA-boxes typical of canonical promoters, and were embedded in nucleosome-free regions flanked by nucleosomes with enhanced levels of histone marks potentially associated with intragenic transcriptional initiation, suggesting that ATI within CDSs was epigenetically tuned and associated with tissue-specific functions. Overall, duplicated genes possessed more TSRs, exhibited lower degrees of tissue specificity, and underwent stronger purifying selection than singletons. This study highlights the significance of ATI and the genomic and epigenomic factors shaping the distribution of ATI in CDSs in a paleopolyploid eukaryote.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142625404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multitasker Argonaute leaves no stone unturned. 多任务处理机 Argonaute 不遗余力。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae306

Laura Arribas-Hernández

引用次数: 0

Metabolic modeling reveals distinct roles of sugars and carboxylic acids in stomatal opening as well as unexpected carbon fluxes. 代谢模型揭示了糖和羧酸在气孔开放中的不同作用，并发现了意想不到的碳通量。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae252

Noah Sprent, C Y Maurice Cheung, Sanu Shameer, R George Ratcliffe, Lee J Sweetlove, Nadine Töpfer

{"title":"Metabolic modeling reveals distinct roles of sugars and carboxylic acids in stomatal opening as well as unexpected carbon fluxes.","authors":"Noah Sprent, C Y Maurice Cheung, Sanu Shameer, R George Ratcliffe, Lee J Sweetlove, Nadine Töpfer","doi":"10.1093/plcell/koae252","DOIUrl":"10.1093/plcell/koae252","url":null,"abstract":"Guard cell metabolism is crucial for stomatal dynamics, but a full understanding of its role is hampered by experimental limitations and the flexible nature of the metabolic network. To tackle this challenge, we constructed a time-resolved stoichiometric model of guard cell metabolism that accounts for energy and osmolyte requirements and which is integrated with the mesophyll. The model resolved distinct roles for starch, sugars, and malate in guard cell metabolism and revealed several unexpected flux patterns in central metabolism. During blue light-mediated stomatal opening, starch breakdown was the most efficient way to generate osmolytes with downregulation of glycolysis allowing starch-derived glucose to accumulate as a cytosolic osmolyte. Maltose could also accumulate as a cytosolic osmoticum, although this made the metabolic system marginally less efficient. The metabolic energy for stomatal opening was predicted to be derived independently of starch, using nocturnally accumulated citrate which was metabolized in the tricarboxylic acid cycle to malate to provide mitochondrial reducing power for ATP synthesis. In white light-mediated stomatal opening, malate transferred reducing equivalents from guard cell photosynthesis to mitochondria for ATP production. Depending on the capacity for guard cell photosynthesis, glycolysis showed little flux during the day but was crucial for energy metabolism at night. In summary, our analyses have corroborated recent findings in Arabidopsis guard cell research, resolved conflicting observations by highlighting the flexibility of guard cell metabolism, and proposed new metabolic flux modes for further experimental testing.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The older the wiser, unless you are a banana: The NAP1-MADS1 network in the regulation of banana ripening. 越老越聪明，除非你是香蕉：香蕉成熟过程中的 NAP1-MADS1 网络调控。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae305

Raul Sanchez-Muñoz

引用次数: 0

Comparative RNA profiling identifies stage-specific phasiRNAs and coexpressed Argonaute genes in Bambusoideae and Pooideae species. 比较 RNA 分析确定了 Bambusoideae 和 Pooideae 物种中特定阶段的 phasiRNA 和共表达 Argonaute 基因。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae308

Sébastien Bélanger, Junpeng Zhan, Yunqing Yu, Blake C Meyers

{"title":"Comparative RNA profiling identifies stage-specific phasiRNAs and coexpressed Argonaute genes in Bambusoideae and Pooideae species.","authors":"Sébastien Bélanger, Junpeng Zhan, Yunqing Yu, Blake C Meyers","doi":"10.1093/plcell/koae308","DOIUrl":"10.1093/plcell/koae308","url":null,"abstract":"Phased, small interfering RNAs (PhasiRNAs) play a crucial role in supporting male fertility in grasses. Earlier work in maize (Zea mays) and rice (Oryza sativa)-and subsequently many other plant species-identified premeiotic 21-nucleotide (nt) and meiotic 24-nt phasiRNAs. More recently, a group of premeiotic 24-nt phasiRNAs was discovered in the anthers of 2 Pooideae species, barley (Hordeum vulgare) and bread wheat (Triticum aestivum). Whether premeiotic 24-nt phasiRNAs and other classes of reproductive phasiRNAs are conserved across Pooideae species remains unclear. We conducted comparative RNA profiling of 3 anther stages in 6 Pooideae species and 1 Bambusoideae species. We observed complex temporal accumulation patterns of 21-nt and 24-nt phasiRNAs in Pooideae and Bambusoideae grasses. In Bambusoideae, 21-nt phasiRNAs accumulated during meiosis, whereas 24-nt phasiRNAs were present in both premeiotic and postmeiotic stages. We identified premeiotic 24-nt phasiRNAs in all 7 species examined. These phasiRNAs exhibit distinct biogenesis mechanisms and potential Argonaute effectors compared to meiotic 24-nt phasiRNAs. We show that specific Argonaute genes coexpressed with stage-specific phasiRNAs are conserved across Bambusoideae and Pooideae species. Our degradome analysis identified a set of conserved miRNA target genes across species, while 21-nt phasiRNA targets were species-specific. Cleavage of few targets was observed for 24-nt phasiRNAs. In summary, this study demonstrates that premeiotic 24-nt phasiRNAs are present across Bambusoideae and Pooideae families, and the temporal accumulation of other classes of 21-nt and 24-nt phasiRNA differs between bamboo and Pooideae species. Furthermore, targets of the 3 classes of phasiRNAs may be rapidly evolving or undetectable.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KNUCKLES regulates floral meristem termination by controlling auxin distribution and cytokinin activity. KNUCKLES 通过控制叶黄素的分布和细胞分裂素的活性来调节花分生组织的终止。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae312

Guangling Wang, Zhiyue Wu, Bo Sun

{"title":"KNUCKLES regulates floral meristem termination by controlling auxin distribution and cytokinin activity.","authors":"Guangling Wang, Zhiyue Wu, Bo Sun","doi":"10.1093/plcell/koae312","DOIUrl":"10.1093/plcell/koae312","url":null,"abstract":"The termination of floral meristem (FM) activity is essential for the normal development of reproductive floral organs. During this process, KNUCKLES (KNU), a C2H2-type zinc finger protein, crucially regulates FM termination by directly repressing the expression of both the stem cell identity gene WUSCHEL (WUS) and the stem cell marker gene CLAVATA3 (CLV3) to abolish the WUS-CLV3 feedback loop required for FM maintenance. In addition, phytohormones auxin and cytokinin are involved in FM regulation. However, whether KNU modulates auxin and cytokinin activities for FM determinacy control remains unclear. Here, we show that the auxin distribution and the cytokinin activity mediated by KNU in Arabidopsis (Arabidopsis thaliana) promote the termination of FM during stage 6 of flower development. Mutation of KNU leads to altered distribution of auxin and cytokinin in the FM of a stage 6 floral bud. Moreover, KNU directly represses the auxin transporter gene PIN-FORMED1 (PIN1) and the cytokinin biosynthesis gene ISOPENTENYLTRANSFERASE7 (IPT7) via mediating H3K27me3 deposition on these 2 loci to regulate auxin and cytokinin activities. Our study presents a molecular regulatory network that elucidates how the transcriptional repressor KNU integrates and modulates the activities of auxin and cytokinin, thus securing the timed FM termination.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Setting the record straight: Loss of wall-associated kinases does not affect plant perception of pectin fragments. 澄清事实：壁相关激酶的丧失并不影响植物对果胶片段的感知。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae318

Leonard Blaschek

引用次数: 0

Correction to: The DYRKP1 kinase regulates cell wall degradation in Chlamydomonas by inducing matrix metalloproteinase expression. 更正：DYRKP1激酶通过诱导基质金属蛋白酶的表达来调节衣藻细胞壁降解。

IF 1 1区生物学

Plant Cell Pub Date : 2024-12-23 DOI: 10.1093/plcell/koae334

引用次数: 0