Plant Cell最新文献_第2页

Cryo-EM structures of Oryza sativa MRP5 reveal a phytate accumulation mechanism in plant vacuoles. 水稻MRP5基因的低温电镜结构揭示了植物液泡中植酸积累的机制。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-04-03 DOI: 10.1093/plcell/koag088

Jiaqi Zuo, Jie Zhang, Ying Tang, Lihuan Jiang, Shuo Cao, Yan Liu, Cuicui Shen, Chuang Wang, Hao Chen, Lizhong Xiong, Ping Yin, Zhou Gong, Zhu Liu

{"title":"Cryo-EM structures of Oryza sativa MRP5 reveal a phytate accumulation mechanism in plant vacuoles.","authors":"Jiaqi Zuo, Jie Zhang, Ying Tang, Lihuan Jiang, Shuo Cao, Yan Liu, Cuicui Shen, Chuang Wang, Hao Chen, Lizhong Xiong, Ping Yin, Zhou Gong, Zhu Liu","doi":"10.1093/plcell/koag088","DOIUrl":"10.1093/plcell/koag088","url":null,"abstract":"Phytate (phytic acid, or InsP6), the primary phosphorus storage compound in plants, plays essential roles in nutrient homeostasis and cellular signaling. However, its strong metal-chelating properties make cytosolic accumulation cytotoxic, necessitating its sequestration into vacuoles for safe storage. Here, we present the cryo-EM structures of the rice vacuolar phytate transporter, OsMRP5, captured in distinct functional states. These structures reveal the molecular basis of OsMRP5 function as an ATP-binding cassette (ABC) transporter. OsMRP5 employs a specialized substrate-recognition mechanism, uniquely adapted to bind the fully hydrophilic InsP6 through extensive electrostatic and hydrogen-bonding interactions within two distinct, highly polar binding sites in its central cavity. A distinctive electropositive tunnel, positioned above the central cavity, forms a continuous pathway connecting the InsP6-binding pocket to the vacuolar export site. This tunnel likely generates an electrostatic attraction that facilitates the movement of the highly anionic InsP6 through the transporter. By mapping mutations from low-phytic acid (lpa) crop variants onto the OsMRP5 structures, we pinpoint their conserved locations critical for transporter function and validate their impact experimentally. These results reveal how OsMRP5 recognizes and transports the highly charged InsP6 molecules into vacuoles, providing a molecular framework for targeted manipulation of this agriculturally important transporter.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RSL4-regulated transcription and ROP signaling coordinate root hair growth via BOUNDARY OF ROP DOMAIN proteins. rsl4调控的转录和ROP信号通过ROP域蛋白的边界协调根毛生长。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-25 DOI: 10.1093/plcell/koag073

Meng Xu, Yu-Qing Li, Xue-Lian Shi, Lu-Shen Li, Guang-Jiu Hao, En Li, Yan Zhang, Sha Li

{"title":"RSL4-regulated transcription and ROP signaling coordinate root hair growth via BOUNDARY OF ROP DOMAIN proteins.","authors":"Meng Xu, Yu-Qing Li, Xue-Lian Shi, Lu-Shen Li, Guang-Jiu Hao, En Li, Yan Zhang, Sha Li","doi":"10.1093/plcell/koag073","DOIUrl":"https://doi.org/10.1093/plcell/koag073","url":null,"abstract":"In vascular plants, root hairs (RH) increase plant adaptability by facilitating the uptake of water and nutrients. RH initiation and elongation require the establishment and maintenance of cellular polarity. The ROOT HAIR DEFECTIVE6-LIKE (RSL) transcription factor RSL4 controls RH-specific gene expression and ROP GTPases direct polar growth, but how these pathways integrate to spatiotemporally orchestrate RH initiation and elongation remains unknown. We identify Arabidopsis BOUNDARY OF ROP DOMAIN (BDR) protein BDR6/7 as positive regulators of RH initiation and growth. BDR6/7 are targeted both to the plasma membrane (PM) and in the nucleus of RHs. By genetic approaches, we demonstrate that ROP signaling is essential for BDR6/7-promoted RH growth whereas ROP2-driven RH growth also requires BDR6/7. BDR6/7 associate and co-localize with RSL2/4 to nuclei in heterologous plant cells. Genetic dissections uncover an interdependency between BDR6/7 and RSL2/4. Indeed, BDR6/7 enhance RSL4-associated RHE reporter activity in protoplasts and support RSL4 binding at target promoters whereas RSL4 binds BDR6/7 promoters and is required for their expression, creating a positive feedback loop that may integrate transcriptional and signaling dynamics. Genetic evidence also supports the dependence of ROP signaling on RSL2/4. The dual-targeted BDR6/7, RSL2/4-mediated transcription, and apical PM-associated ROP signaling, may form a tripartite module, scaling developmental outputs for spatiotemporal coordination of plant cell morphogenesis.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reply: Neutral transcriptome rewiring promotes quantitative disease resistance evolvability at the species level. 回复：中性转录组重接线促进物种水平上的定量抗病进化。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-16 DOI: 10.1093/plcell/koag068

Florent Delplace, Mehdi Khafif, Adelin Barbacci, Remco Stam, Sylvain Raffaele

引用次数: 0

Substrate specificity and transport mechanism of the chloroplast dicarboxylate transporters DiT1 and DiT2. 叶绿体二羧酸转运体DiT1和DiT2的底物特异性和转运机制。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag041

Zhao Yang, Xue Zhang, Jingtao Zheng, Shunhao Zhou, Ming-Ju Amy Lyu, Miaolian Ma, Xin-Guang Zhu, Fang Yu, Peng Zhang

{"title":"Substrate specificity and transport mechanism of the chloroplast dicarboxylate transporters DiT1 and DiT2.","authors":"Zhao Yang, Xue Zhang, Jingtao Zheng, Shunhao Zhou, Ming-Ju Amy Lyu, Miaolian Ma, Xin-Guang Zhu, Fang Yu, Peng Zhang","doi":"10.1093/plcell/koag041","DOIUrl":"10.1093/plcell/koag041","url":null,"abstract":"Dicarboxylate transporters (DiTs) mediate the exchange of dicarboxylates across the chloroplast inner membrane, playing critical roles in C/N coupling, photorespiration, chloroplast redox homeostasis, and C4 photosynthesis. DiT1 and DiT2 are Na⁺-independent exchangers of the solute carrier 13 (SLC13) family, and exhibit overlapping yet distinct substrate specificities: DiT1 transports 2-oxoglutarate, malate, and oxaloacetate, while DiT2 additionally transports glutamate and aspartate. However, the structural determinants of their substrate specificity and transport mechanism remain unclear. Here, we determined cryo-electron microscopy structures of Arabidopsis thaliana DiT1 and DiT2.1 bound to diverse substrates in dual conformational states. Structural analyses revealed that AtDiT1 possesses a singular dicarboxylate-binding site that is electrostatically incompatible with amino acid substrates, whereas AtDiT2.1 has 2 distinct sites to accommodate C4- and C5-dicarboxylates, thus allowing amino acids to bind without electrostatic repulsion. Phylogenetic analysis identified an A226S substitution in the substrate-binding site of DiT1, emerging during evolution in the charophyte ancestor of land plants. This substitution enhances oxaloacetate binding affinity in DiT1, which may have improved adaptation to terrestrial environments. Additionally, 2 conserved positively charged residues in DiTs functionally mimic Na⁺ used by SLC13 co-transporters, thereby enabling a Na⁺-independent elevator-type transport mechanism. These findings provide critical structural and mechanistic insights into the functional divergence of plant DiTs.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Organization, genomic targeting, and assembly of three distinct SWI/SNF chromatin remodeling complexes in Arabidopsis. 修正：拟南芥中三种不同SWI/SNF染色质重塑复合物的组织、基因组靶向和组装。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag079

引用次数: 0

Replication timing uncovers a two-compartment nuclear architecture of interphase euchromatin. 复制时间揭示了间期常染色质的双室核结构。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag042

H S Akram, E E Wear, L Mickelson-Young, Z M Turpin, L Hanley-Bowdoin, W F Thompson, L Concia, H W Bass

{"title":"Replication timing uncovers a two-compartment nuclear architecture of interphase euchromatin.","authors":"H S Akram, E E Wear, L Mickelson-Young, Z M Turpin, L Hanley-Bowdoin, W F Thompson, L Concia, H W Bass","doi":"10.1093/plcell/koag042","DOIUrl":"10.1093/plcell/koag042","url":null,"abstract":"Genome replication is temporally regulated during S phase, with specific genomic regions replicating at defined times in a process that is known as replication timing (RT). Based on 3D cytology in replicating nuclei, we previously proposed a model in which maize euchromatin is subdivided into subcompartments distinguished by chromatin condensation and RT. However, whether this compartmentalization reflects a general nuclear architecture that persists throughout the cell cycle was unclear. To test this model, we conducted two orthogonal assays-Hi-C for genome-wide interaction data and 3D FISH for direct visualization of chromatin organization in maize (Zea mays L.). Hi-C analyses revealed distinct patterns of early-S regions exhibited negative insulation scores with long-range contacts, whereas middle-S regions showed the opposite. Early-S regions showed the strongest correlation with epigenomic signatures of open, transcriptionally active chromatin. 3D oligo FISH painting confirmed that early-S and middle-S replicating regions occupy adjacent but largely non-overlapping nucleoplasmic sub-territories throughout interphase stages, including G1. Together, our findings redefine the maize euchromatin \"A\" compartment as two spatially distinct subcompartments derived from high-frequency RT transitions between early and middle S along the linear genome. These findings have implications for chromatin-templated processes and underscore the importance of RT as a defining feature of genome organization.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13017713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Domain architecture of plant eukaryotic translation initiation factor 3 subunit E governs interaction with translational cis-elements to regulate pollen tube growth. 修正：植物真核翻译起始因子3亚基E的结构域结构控制着与翻译顺式元件的相互作用以调节花粉管生长。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag084

引用次数: 0

Straight outta clusters: differing gene duplication patterns for alkaloid biosynthesis and floral traits in California poppy. 直接出簇：加利福尼亚罂粟生物碱生物合成和花性状的不同基因复制模式。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag051

Linhan Sun

引用次数: 0

CONSTANS-LIKE 5: a key regulator of flower opening and scent emission in Nicotiana attenuata and Petunia axillaris. CONSTANS-LIKE 5：凋零烟草和矮牵牛花开放和香味释放的关键调控因子。

IF 11.6 1区生物学