Plant Cell最新文献_第2页

Expression of Concern: Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA. 表达关注：泛素连接酶RGLG1和RGLG5通过控制磷酸酶PP2CA的周转来调节脱落酸信号。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-08-04 DOI: 10.1093/plcell/koaf192

引用次数: 0

The Arabidopsis histone methylation reader MRG2 interacts with eIF4A3 to regulate alternative splicing and circadian rhythms. 拟南芥组蛋白甲基化解读器MRG2与eIF4A3相互作用，调节选择性剪接和昼夜节律。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-08-04 DOI: 10.1093/plcell/koaf209

Yaxue Huang, Jiabing Wu, Xiang Li, Jiachen Wang, Mengmeng Ma, Wen Jiang, Wen-Hui Shen, Yu Yu, Aiwu Dong

{"title":"The Arabidopsis histone methylation reader MRG2 interacts with eIF4A3 to regulate alternative splicing and circadian rhythms.","authors":"Yaxue Huang, Jiabing Wu, Xiang Li, Jiachen Wang, Mengmeng Ma, Wen Jiang, Wen-Hui Shen, Yu Yu, Aiwu Dong","doi":"10.1093/plcell/koaf209","DOIUrl":"10.1093/plcell/koaf209","url":null,"abstract":"Alternative splicing (AS) is an important regulatory mechanism for fine-tuning gene transcription in eukaryotes. H3K36me3 affects AS, but the underlying mechanisms remain obscure. In this study, we showed that the Arabidopsis thaliana H3K36me3 reader protein MORF-RELATED GENE 2 (MRG2) directly interacts with eIF4A3, a component of the exon junction complex within the spliceosome. The eif4a3 mutant displays a late-flowering phenotype similar to that of the mrg1 mrg2 double mutant under long-day, but not short-day, photoperiod conditions. Transcriptome analysis showed that deleting either eIF4A3 or MRG1/MRG2 causes similar changes in gene transcription and AS, which are involved in diverse processes including circadian rhythm regulation and responses to environmental stimuli. Both eIF4A3 and MRG1/MRG2 are required for the AS of key circadian clock genes and the maintenance of an appropriate circadian rhythm. RNA immunoprecipitation sequencing (RIP-seq) showed that MRG1/MRG2 promote eIF4A3 binding to the transcripts of a set of genes, including the key circadian clock genes PSEUDO-RESPONSE REGULATOR 7 (PRR7) and PRR9. Moreover, eIF4A3 and MRG2 directly target and enhance each other's binding to PRR7 and PRR9 chromatin. Collectively, our findings reveal that the reader protein MRG2 recognizes histone methylation signals and recruits eIF4A3 to regulate co-transcriptional AS events, establishing a direct link between histone modification and the splicing machinery in plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dark-responsive BGH2 and light-responsive BPG4: Taming the GLK1/2 master transcription factors for etioplast and chloroplast homeostasis. 暗适应BGH2和光响应BPG4：抑制GLK1/2主转录因子对病质体和叶绿体稳态的影响。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-08-04 DOI: 10.1093/plcell/koaf190

Jiajun Wang

引用次数: 0

The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets. 荷荷巴脂滴蛋白LDAP1促进蜡酯包装成脂滴。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-08-01 DOI: 10.1093/plcell/koaf115

Payton Whitehead, Saad Raza, Magdalena Miklaszewska, Ellen Hornung, Cornelia Herrfurth, Rohith Nadella, Alyssa Clews, Nathan M Doner, John M Dyer, Robert Mullen, Ivo Feussner, Josh V Vermaas, Kent D Chapman

{"title":"The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid droplets.","authors":"Payton Whitehead, Saad Raza, Magdalena Miklaszewska, Ellen Hornung, Cornelia Herrfurth, Rohith Nadella, Alyssa Clews, Nathan M Doner, John M Dyer, Robert Mullen, Ivo Feussner, Josh V Vermaas, Kent D Chapman","doi":"10.1093/plcell/koaf115","DOIUrl":"10.1093/plcell/koaf115","url":null,"abstract":"Jojoba (Simmondsia chinensis) is a desert shrub with an unusual capacity to store liquid wax esters (WEs) in its seeds instead of triacylglycerols (TAGs) like most oilseed crops. To examine the factors that are important for WE compartmentalization in jojoba, we reconstituted WE biosynthesis and packaging in the leaves of Nicotiana benthamiana. Using this system, we screened jojoba proteins for their ability to support lipid droplet (LD) formation. A specific LIPID DROPLET-ASSOCIATED PROTEIN (LDAP) isoform, ScLDAP1, was identified as a key factor in the efficient compartmentalization of WEs in plant cells. LDAP1 isoforms from other plants (e.g. Arabidopsis thaliana [AtLDAP1]) did not support WE partitioning from the endoplasmic reticulum into LDs, although both AtLDAP1 and ScLDAP1 were targeted specifically to LD monolayer surfaces. ScLDAP1-mediated selective, efficient WE partitioning was facilitated by an amphipathic α-helix near its C-terminus, and mutational analysis identified 1 amino acid residue within this helix that was both necessary and sufficient for proper WE packaging into cytoplasmic LDs. Taken together, our results provide a mechanistic link between the biosynthesis and storage of WEs in plant cells, and will inform future biotechnology strategies for the efficient packaging of various neutral lipid types as demonstrated here for WEs in transgenic seeds.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"37 8","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12341951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144837317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar, we're going down: PRC2-mediated epigenetic repression of sucrose metabolism promotes phototrophy during seedling establishment. 糖，我们正在下降：prc2介导的蔗糖代谢的表观遗传抑制在幼苗建立期间促进光养。

IF 1 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf155

Rory Osborne

引用次数: 0

Tiny seeds, big decisions: Jasmonate-mediated regulation of seed size in Arabidopsis via the SOD7-KLU module. 小种子，大决定：茉莉酸通过SOD7- KLU模块介导拟南芥种子大小的调节。

IF 1 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf160

Nitin Uttam Kamble

引用次数: 0

Best practices in plant fluorescence imaging and reporting: A primer. 植物荧光成像和报告的最佳实践：引物。

IF 11.6 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf143

Kirk J Czymmek, Yoselin Benitez-Alfonso, Tessa Burch-Smith, Luigi F Di Costanzo, Georgia Drakakaki, Michelle Facette, Daniel Kierzkowski, Anastasiya Klebanovych, Ivan Radin, Suruchi Roychoudhry, Heather E McFarlane

{"title":"Best practices in plant fluorescence imaging and reporting: A primer.","authors":"Kirk J Czymmek, Yoselin Benitez-Alfonso, Tessa Burch-Smith, Luigi F Di Costanzo, Georgia Drakakaki, Michelle Facette, Daniel Kierzkowski, Anastasiya Klebanovych, Ivan Radin, Suruchi Roychoudhry, Heather E McFarlane","doi":"10.1093/plcell/koaf143","DOIUrl":"10.1093/plcell/koaf143","url":null,"abstract":"Microscopy is a fundamental approach for plant cell and developmental biology as well as an essential tool for mechanistic studies in plant research. However, setting up a new microscopy-based experiment can be challenging, especially for beginner users, when implementing new imaging workflows or when working in an imaging facility where staff may not have extensive experience with plant samples. The basic principles of optics, chemistry, imaging, and data handling are shared among all cell types. However, unique challenges are faced when imaging plant specimens due to their waxy cuticles, strong/broad spectrum autofluorescence, recalcitrant cell walls, and air spaces that impede fixation or live imaging, impacting sample preparation and image quality. As expert plant microscopists, we share our collective experience on best practices to improve the quality of published microscopy results and promote transparency, reproducibility, and data reuse for meta-analyses. We offer plant-specific advice and examples for microscope users at all stages of fluorescence microscopy workflows, from experimental design through sample preparation, image acquisition, processing, and analyses, to image display and methods reporting in manuscripts. We also present standards for methods reporting that will be valuable to all users and offer tools to improve reproducibility and data sharing.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"37 7","pages":""},"PeriodicalIF":11.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence and evolution of canonical microRNAs: A case study in Arabidopsis halleri and A. lyrata. 标准microrna的出现和进化：以拟南芥和拟南芥为例。

IF 1 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf159

Pei Qin Ng

引用次数: 0

Chromatin insulators: Good fences that make good neighbors. 染色质绝缘体：形成好邻居的好篱笆。

IF 1 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf157

Laura Arribas-Hernández

引用次数: 0

The multifunctional ascorbate peroxidase MoApx1 secreted by Magnaporthe oryzae mediates the suppression of rice immunity. 水稻Magnaporthe oryzae分泌的多功能抗坏血酸过氧化物酶MoApx1介导水稻免疫抑制。

IF 1 1区生物学

Plant Cell Pub Date : 2025-07-01 DOI: 10.1093/plcell/koaf146

Muxing Liu, Ziqian Guo, Jiexiong Hu, Yuke Chen, Fang Chen, Weizhong Chen, Wenya Wang, Boyang Ye, Zhixiang Yang, Gang Li, Xinyu Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang

{"title":"The multifunctional ascorbate peroxidase MoApx1 secreted by Magnaporthe oryzae mediates the suppression of rice immunity.","authors":"Muxing Liu, Ziqian Guo, Jiexiong Hu, Yuke Chen, Fang Chen, Weizhong Chen, Wenya Wang, Boyang Ye, Zhixiang Yang, Gang Li, Xinyu Liu, Haifeng Zhang, Ping Wang, Zhengguang Zhang","doi":"10.1093/plcell/koaf146","DOIUrl":"10.1093/plcell/koaf146","url":null,"abstract":"Fungi secrete effector proteins, including extracellular redox enzymes, to inhibit host immunity. Redox enzymes have been hypothesized to inhibit host reactive oxygen species (ROS); however, how they suppress host immunity remains unknown. We characterized an extracellular ascorbate peroxidase (MoApx1) that is secreted into rice chloroplasts by the rice blast fungus Magnaporthe oryzae. MoApx1 displays multifunctional capabilities that significantly contribute to fungal virulence. Firstly, MoApx1 neutralizes host-derived H2O2 within the chloroplast through its peroxidase activity, thereby inhibiting chloroplast ROS (cROS)-mediated defense responses. Secondly, MoApx1 targets the photosystem I subunit OsPsaD, disrupting photosynthetic electron transport to further suppress cROS production. Most importantly, MoApx1 has evolved a fungal-specific starch-binding domain that binds host starch, inhibiting its degradation and disrupting the energy supply required for host resistance. Our findings underscore the importance of a novel multifaceted strategy, potentially widely employed by other fungal pathogens, in suppressing host immunity during host-microbe interactions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":10.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0