Plant Cell最新文献_第3页

Straight outta clusters: differing gene duplication patterns for alkaloid biosynthesis and floral traits in California poppy. 直接出簇：加利福尼亚罂粟生物碱生物合成和花性状的不同基因复制模式。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag051

Linhan Sun

引用次数: 0

Dynamic chromatin regulatory programs of sucrose and citric acid metabolism during fruit ripening in Citrus. 柑橘果实成熟过程中蔗糖和柠檬酸代谢的动态染色质调控程序。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag060

Xin Song, Ting-Ting Wang, Peng Zhao, Li-Gang He, Yan-Jie Fan, Yu Zhang, Ting Liu, Chun-Mei Shi, Ying-Chun Jiang, Feng-Quan Tan, Abdelhafid Bendahmane, Chun-Long Li, Li-Ming Wu, Fang Song

{"title":"Dynamic chromatin regulatory programs of sucrose and citric acid metabolism during fruit ripening in Citrus.","authors":"Xin Song, Ting-Ting Wang, Peng Zhao, Li-Gang He, Yan-Jie Fan, Yu Zhang, Ting Liu, Chun-Mei Shi, Ying-Chun Jiang, Feng-Quan Tan, Abdelhafid Bendahmane, Chun-Long Li, Li-Ming Wu, Fang Song","doi":"10.1093/plcell/koag060","DOIUrl":"10.1093/plcell/koag060","url":null,"abstract":"Fruit ripening is a complex, tightly regulated process that affects fruit flavor, nutritional value, and shelf life. Citrus is a typical non-climacteric fruit, as citrus fruits ripen independently of ethylene. However, the precise regulatory mechanism underlying this process remains to be elucidated. To dissect the epigenetic and transcriptional basis of citrus ripening, we performed integrative analyses of genome-wide histone modifications (H3K27ac, H3K4me3, and H3K27me3), chromatin accessibility, and transcriptome profiles throughout fruit ripening in Citrus reticulata. We constructed a transcriptional regulatory network encompassing 52 key transcription factors that orchestrate citrus fruit ripening. Further, we identified the key role of abscisic acid (ABA) in modulating sucrose and citric acid metabolism and uncovered the underlying transcriptional and epigenetic regulatory network during fruit ripening in C. reticulata. The findings demonstrate that CrHSFA6B and CrbZIP5b interact with the promoters of CrACO1 (Aconitase 1) and CrACO3 and CrSPSF1 (Sucrose Phosphate Synthase F1), regulating their expression to affect the sucrose and citric acid metabolism and fruit ripening. This study provides insights into the epigenomic dynamics of citrus fruit ripening and identifies a putative regulatory cascade centered on ABA, CrbZIP5b, and CrHSFA6B that modulates key processes in citrus ripening.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 3","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13014036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147514262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-layered disruption: how calcium, acidification, and ROS signals converge to shut down energy metabolism in incompatible pollen. 多层破坏：钙、酸化和ROS信号如何汇聚以关闭不相容花粉的能量代谢。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag046

Ved Prakash

引用次数: 0

Chloroplast ionome characterization provides new avenues to shape organellar iron homeostasis. 叶绿体离子单体的表征为塑造细胞器铁稳态提供了新的途径。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-03-03 DOI: 10.1093/plcell/koag027

Matteo Pivato

引用次数: 0

Lights out: PP2C phosphatases reset phototropin signalling in darkness. 熄灯：PP2C磷酸酶在黑暗中重置促光素信号。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-26 DOI: 10.1093/plcell/koag049

Barbara Bourgade

引用次数: 0

Chromatin gatekeeping at cooler temperature: VIL1 modulates CBF- driven growth in Arabidopsis. 低温下的染色质守门人：VIL1调节CBF驱动的拟南芥生长。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-20 DOI: 10.1093/plcell/koag044

Crispus M Mbaluto

引用次数: 0

FeM-ID: a biotin labeling-based approach for the dissection of female meiotic chromosome behavior in Arabidopsis thaliana. FeM-ID：一种基于生物素标记的方法来解剖拟南芥雌性减数分裂染色体行为。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-16 DOI: 10.1093/plcell/koag024

Chao Feng, Baicui Wang, Maria Cuacos, Jana Lorenz, Franziska Hartmann, Stefan Heckmann

{"title":"FeM-ID: a biotin labeling-based approach for the dissection of female meiotic chromosome behavior in Arabidopsis thaliana.","authors":"Chao Feng, Baicui Wang, Maria Cuacos, Jana Lorenz, Franziska Hartmann, Stefan Heckmann","doi":"10.1093/plcell/koag024","DOIUrl":"10.1093/plcell/koag024","url":null,"abstract":"Meiosis assures formation of both male and female gametes. However, cytological studies of female meiotic chromosome behavior in plants are scarce, mainly due to the comparatively low number and inaccessibility of female meiotic cells. We present Female Meiotic cell IDentification (FeM-ID), an approach for cytological identification of female meiotic cells in Arabidopsis. By employing ASY1-eYFP-TurboID, female meiotic cells (chromatin and cytoplasm) are specifically labeled by biotin. This facilitates their rapid microscopic identification and detailed assessment of female meiotic chromosome behavior. ASY1-eYFP-TurboID can be transformed or introgressed into different genetic backgrounds without any adverse effects on meiosis in either sex. Our approach enabled us to analyze female meiotic chromosome behavior, including bivalent and univalent frequencies, minimum chiasma numbers and chromosome segregation, in various ecotypes (Col-0, Ler-0, and Ws-2), colchicine-induced tetraploids (Col-4x) and selected meiotic mutants (sporulation 11-1 (spo11-1), meiotic recombination 11 (mre11), and mutL homolog 3 (mlh3), facilitating cytological studies of heterochiasmy (sex-specific differences in recombination rates or patterns). FeM-ID is compatible with other cytological techniques, such as immunolocalization and fluorescence in situ hybridization, which allowed us, for instance, to assess homologous chromosome pairing or to track individual chromosomes in female meiotic cells. In conclusion, FeM-ID represents an approach that fills a long-standing gap in the dissection of female meiotic chromosome behavior in Arabidopsis and holds the potential for application in other plant species.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-guided discovery of protein functions in plants. 以结构为导向的植物蛋白质功能发现。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-16 DOI: 10.1093/plcell/koag022

Jiarong Chen, Yanlei Feng, Yuchan Zhang, Jucan Gao, Jinda Ou, Weiyin Wu, Can Li, Shuyan Song, Li Tai, Mahmudul Hasan Rifat, Delara Akhter, Jianping Hu, Peiqiang Feng, Xing-Xing Shen, Ronghui Pan

{"title":"Structure-guided discovery of protein functions in plants.","authors":"Jiarong Chen, Yanlei Feng, Yuchan Zhang, Jucan Gao, Jinda Ou, Weiyin Wu, Can Li, Shuyan Song, Li Tai, Mahmudul Hasan Rifat, Delara Akhter, Jianping Hu, Peiqiang Feng, Xing-Xing Shen, Ronghui Pan","doi":"10.1093/plcell/koag022","DOIUrl":"10.1093/plcell/koag022","url":null,"abstract":"Protein structure serves as a critical bridge between sequence and functional annotation, particularly in establishing functional links among distantly homologous proteins with low sequence similarities. However, systematic protein structure-based functional annotations have been lacking in plants, where functions for a significant portion of the proteomes are still elusive. In this study, we leveraged protein structural data from 17 angiosperms to uncover previously unannotated protein functions in plants. After structural clustering, we used the plant clusters to query the UniProtKB/Swiss-Prot database (the expertly curated component of UniProtKB), a repository of expertly curated and reliably annotated proteins, and identified structural matches for thousands of plant clusters that were undetectable by sequence-based BLAST searches. We further selected 120 clusters, which are highly reliable in structural quality and alignment and are well-conserved across plant species, and uncovered various protein functions that are potentially widely important in plants. Finally, we experimentally analyzed one plant cluster structurally resembling the yeast peroxisomal peroxin 8 (PEX8) protein and verified that plant PEX8-like proteins can functionally complement yeast pex8 mutants. Our findings highlight the power of structural comparison in uncovering protein functions in plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The rust effector PstCFEM2 manipulates TaHA2- and TaCIPK9-mediated apoplastic acidification to promote wheat susceptibility. 锈病效应因子PstCFEM2操纵TaHA2和tacipk9介导的外胞体酸化，促进小麦易感性。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-16 DOI: 10.1093/plcell/koag021

Yanqin Zhang, Shuangyuan Guo, Longhui Yu, Yi Lin, Haoshan Liu, Xueling Huang, Chunlei Tang, Xiaojie Wang, Zhensheng Kang, Xinmei Zhang

{"title":"The rust effector PstCFEM2 manipulates TaHA2- and TaCIPK9-mediated apoplastic acidification to promote wheat susceptibility.","authors":"Yanqin Zhang, Shuangyuan Guo, Longhui Yu, Yi Lin, Haoshan Liu, Xueling Huang, Chunlei Tang, Xiaojie Wang, Zhensheng Kang, Xinmei Zhang","doi":"10.1093/plcell/koag021","DOIUrl":"10.1093/plcell/koag021","url":null,"abstract":"Apoplastic acidification represents a pivotal mechanism in the co-evolutionary dynamics between plants and pathogens. However, the mechanisms underlying this process remain largely uncharacterized. In this study, we unveil a mechanism by which the stripe rust fungal (Puccinia striiformis f. sp. tritici; Pst) effector manipulates plasma membrane (PM) H+-ATPases to promote apoplastic acidification and attenuate host immune responses. We identified a wheat (Triticum aestivum) PM H+-ATPase (TaHA2) as a key regulator of apoplastic pH and defense responses to Pst infection. The overexpression of TaHA2 exacerbated apoplastic acidification and Pst susceptibility, whereas the CRISPR-Cas9-mediated inactivation of TaHA2 in wheat conferred broad-spectrum resistance against multiple rust pathogens without compromising agronomic traits. Mechanistically, we found that the wheat calcineurin B-like interacting protein kinase 9 (TaCIPK9) phosphorylates TaHA2 at Ser-933, triggering intramolecular interactions between its C-terminal autoinhibitory domain and the central loop, thereby suppressing TaHA2 activity. Conversely, the CFEM (common in fungal extracellular membrane)-containing Pst effector PstCFEM2 competitively binds to the C-terminus of TaHA2, disrupting TaCIPK9-mediated phosphorylation and relieving autoinhibition. This effector-driven activation of TaHA2 amplifies apoplastic acidification and stomatal opening, ultimately dampening plant immunity. Our findings reveal a mechanism by which pathogens promote infection by subverting host pH regulation and provide a theoretical framework for engineering disease resistance through the manipulation of susceptibility genes.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis transcription factor TCP4 controls seed size by repressing the MINI3-SHB1-IKU pathway. 拟南芥转录因子TCP4通过抑制MINI3-SHB1-IKU通路控制种子大小。

IF 11.6 1区生物学

Plant Cell Pub Date : 2026-02-16 DOI: 10.1093/plcell/koag020

Zeliang Zhang, Yiyi Li, Hongjun Long, Yu Cao, Xinxue Wang, Genji Qin

{"title":"The Arabidopsis transcription factor TCP4 controls seed size by repressing the MINI3-SHB1-IKU pathway.","authors":"Zeliang Zhang, Yiyi Li, Hongjun Long, Yu Cao, Xinxue Wang, Genji Qin","doi":"10.1093/plcell/koag020","DOIUrl":"10.1093/plcell/koag020","url":null,"abstract":"As an important agronomic trait that directly determines grain yield, seed size is tightly controlled by the timing of endosperm cellularization and seed coat proliferation. However, the precise regulatory mechanisms that coordinate the two processes remain elusive. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), CINCINNATA (CIN)-like TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors are crucial for seed size by controlling both endosperm cellularization and seed coat growth. Disruption of TCP function in the triple mutant tcp3/4/10 causes enlarged seeds due to the delayed endosperm cellularization and accelerated seed coat growth. TCP4 directly represses MINISEED3 (MINI3) and SHORT HYPOCOTYL UNDER BLUE1 (SHB1), key factors for endosperm cellularization, by recruiting the FERTILIZATION INDEPENDENT SEED-POLYCOMB REPRESSIVE COMPLEX 2 (FIS-PRC2) complex. TCP4 also interacts with MINI3 to repress MINI3-SHB1 complex activity, thereby suppressing the expression of MINI3 downstream genes. We further show that TCP4 represses the expression of AINTEGUMENTA (ANT), a key regulator of integument and seed coat growth, by directly binding to its promoter. Our findings demonstrate that CIN-like TCPs play critical roles in controlling seed size by promoting endosperm cellularization and concurrently inhibiting seed coat proliferation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":11.6,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0