Plant Cell最新文献_第3页

Timing it right: dephosphorylation of a key transcription factor constrains the tapetal transcriptional cascade for timely pollen development. 正确的时间：一个关键转录因子的去磷酸化限制了绒毡层转录级联，以及时花粉发育。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag294

Pablo González-Suárez, Cecilia Lara-Mondragón

引用次数: 0

Sorbitol dehydrogenase aids grain fill and integrates carbohydrate metabolism with redox in the hypoxic maize endosperm. 山梨糖醇脱氢酶有助于玉米籽粒灌浆，并在缺氧的玉米胚乳中整合碳水化合物代谢和氧化还原。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag293

Nadia Mourad, Hardy Rolletschek, Matthias Langer, Maria Angelica Sanclemente, Michelle Saint-Fleur, Lily Herndon, Shan Wu, Sylvia Morais de Sousa Tinôco, Jiahn-Chou Guan, Karen E Koch

{"title":"Sorbitol dehydrogenase aids grain fill and integrates carbohydrate metabolism with redox in the hypoxic maize endosperm.","authors":"Nadia Mourad, Hardy Rolletschek, Matthias Langer, Maria Angelica Sanclemente, Michelle Saint-Fleur, Lily Herndon, Shan Wu, Sylvia Morais de Sousa Tinôco, Jiahn-Chou Guan, Karen E Koch","doi":"10.1093/plcell/koag293","DOIUrl":"10.1093/plcell/koag293","url":null,"abstract":"Extreme low oxygen is typical of microenvironments inside developing grains, thus requiring metabolic adaptations to hypoxia. Potential contributions from sorbitol dehydrogenase (SDH) are tested here for maize (Zea mays) since the reversible reaction (fructose + NADH ↔ sorbitol + NAD+) can balance both redox state and metabolism during assimilate import into kernels. To do so, we developed and analyzed maize sdh1 mutants and overexpression lines. Dysfunction of Sdh1 decreased seed weight by 17%, impaired starch accumulation, and enhanced levels of glucose, fructose, and sucrose. Redox balance in the inner endosperm was also disrupted (NADH and NADPH levels rose), and adenylate energy charge decreased. Moreover, genes responsive to oxidative stress were upregulated in mutant kernels. In contrast, Sdh1-OE lines rescued the small-grain phenotype without exceeding wild-type kernel size. Metabolite profiles of mutant and Sdh1-OE kernels revealed reciprocal levels for sucrose, hexoses, and key points of redox sensitivity and C/N balance. Results are distinct from the classical involvement of sorbitol in long-distance transport (as in apples) and its contribution to desiccation tolerance in diverse species. Rather, our analyses support a 2-fold contribution by an SDH-modulated sorbitol reservoir that (i) aids redox balance in the hypoxic endosperm by storing excess reductant and (ii) minimizes fructose levels that can otherwise inhibit sucrose import, metabolism, and signaling pathways. Collective evidence reveals a previously unrecognized role for sorbitol and SDH in kernel-fill that integrates carbohydrate metabolism and redox balance in the low-oxygen endosperm of this developing grain.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species. 植物光感受器UVR8的磷酸化调节其二聚体/单体状态，并增强物种间的UV-B信号传导。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-10-05 DOI: 10.1093/plcell/koag285

Boqin Tong, MeLongying Wangzha, Jie Wang, Baoguo Wu, Chunli Zhang, András Viczián, Assaf Mosquna, Yizhuo Mu, Bingbing Wan, Li Lin, Ruohe Yin

{"title":"Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species.","authors":"Boqin Tong, MeLongying Wangzha, Jie Wang, Baoguo Wu, Chunli Zhang, András Viczián, Assaf Mosquna, Yizhuo Mu, Bingbing Wan, Li Lin, Ruohe Yin","doi":"10.1093/plcell/koag285","DOIUrl":"10.1093/plcell/koag285","url":null,"abstract":"Ultraviolet-B (UV-B) is an integral component of sunlight that profoundly shapes plant growth, development, and survival. Plant photoreceptor UV RESISTANCE LOCUS 8 (UVR8) homodimers perceive UV-B and dissociate into active monomers to initiate signaling, yet how UVR8 dimer stability and monomer activity are post-translationally regulated remains unclear. We identify UV-B-enhanced phosphorylation at threonine 356 of UVR8, which promotes UV-B-induced UVR8 dimer dissociation and delays redimerization, shifting dimer/monomer equilibrium towards active monomer in Arabidopsis. Importantly, the same regulatory mechanism is conserved in tomato, demonstrating that phosphorylation at this site represents a broadly applicable molecular switch across species. Moreover, this modification enhances the binding of UVR8 to CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), prolongs UVR8 nuclear residence, and amplifies UV-B signaling outputs. Together, these findings uncover phosphorylation at T356 as an evolutionarily conserved molecular switch that fine-tunes photoreceptor conformational change and signaling, highlighting phosphorylation as a critical posttranslational control of light perception in plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 10","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mobile lncRNA QingLattice mediates DML-IDN2 crosstalk via chromatin looping to reprogram DNA methylation and nutrient allocation. 一个可移动的lncRNA QingLattice通过染色质环介导DML-IDN2串扰，从而重编程DNA甲基化和营养分配。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-28 DOI: 10.1093/plcell/koag303

Ce Wang, Zhinxin Ren, Mengmeng Fu, Xiaoke Zhang, Zhen Xu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu

{"title":"A mobile lncRNA QingLattice mediates DML-IDN2 crosstalk via chromatin looping to reprogram DNA methylation and nutrient allocation.","authors":"Ce Wang, Zhinxin Ren, Mengmeng Fu, Xiaoke Zhang, Zhen Xu, Ji Tian, Yi Wang, Xinzhong Zhang, Zhenhai Han, Ting Wu","doi":"10.1093/plcell/koag303","DOIUrl":"https://doi.org/10.1093/plcell/koag303","url":null,"abstract":"Vascular-mobile lncRNAs can function as systemic signals in abiotic stress adaptation. However, their direct molecular mechanisms-particularly the ability to recruit chromatin remodelers for modulating chromatin architecture at target loci-remain largely unexplored in sink tissues, where chromatin accessibility and transcriptional reprogramming are critical for systemic adaptation. Here, we show that the lncRNA QingLattice acts as a mobile signal during iron (Fe) deficiency, moving from shoot to root tissues where it reprograms target gene expression through two synergistic epigenetic pathways. Directly, QingLattice binds the MdNRAMP3 locus and recruits the demethylase MdDML1, catalyzing active DNA demethylation to activate MdNRAMP3 transcription. Indirectly, QingLattice induces alteration of chromatin looping at the MdIDN2 locus, repressing this core RdDM component and thereby relieving RdDM-mediated methylation suppression on MdNRAMP3. These two pathways coordinately reduce MdNRAMP3 methylation and ensure its robust expression, leading to enhanced vacuolar Fe export and systemic Fe-deficiency tolerance. Grafting experiments with QingLattice-overexpressing scions and wild-type rootstocks confirm that its mobility is necessary and sufficient for these epigenetic regulation-induced adaptive phenotypes. Our findings reveal a paradigm in which a mobile lncRNA integrates direct demethylase recruitment with indirect RdDM suppression to fine-tune target gene methylation, offering insight into systemic epigenetic regulation of plant-environment interactions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRANSPORTIN1 coordinates plant immune activation by mediating nuclear import of ADR1 helper NLRs. TRANSPORTIN1通过介导ADR1辅助NLRs的核输入来协调植物免疫激活。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-19 DOI: 10.1093/plcell/koag290

Jianyu Li, Jianhua Huang, Yujie Jin, Jan Sklenar, Jonathan D G Jones, Hailong Guo

引用次数: 0

Somatic selection shapes mutation accumulation in Citrus sinensis. 体细胞选择影响柑桔突变积累。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-18 DOI: 10.1093/plcell/koag288

Taylor R Beaulieu, Emmanuel Ávila de Dios, Danelle K Seymour

{"title":"Somatic selection shapes mutation accumulation in Citrus sinensis.","authors":"Taylor R Beaulieu, Emmanuel Ávila de Dios, Danelle K Seymour","doi":"10.1093/plcell/koag288","DOIUrl":"https://doi.org/10.1093/plcell/koag288","url":null,"abstract":"An organism becomes genetically mosaic through the accumulation of somatic mutations. Genetic mosaicism is a commonality of multicellular life and has been studied extensively in humans due to its associations with aging and diseases. In humans, somatic selection shapes the accumulation of somatic mutations, with strong signatures of positive somatic selection in cancer cell lineages. So far, evidence for somatic selection in plants has been inconsistent. The evolutionary implications of genetic mosaicism in humans and other animals are limited by early specification of germline cells, preventing transmission of somatic mutations to progeny. In contrast, many plant lineages reproduce asexually with clonal progeny derived from vegetative tissues. We describe the patterns and processes shaping somatic mutation accumulation within a single, 149-year-old historic sweet orange (Citrus sinensis) tree and within a clonal lineage of sweet orange. More than 12,000 somatic mutations were identified in the historic tree and 28,000 somatic mutations were identified across 199 clonally related sweet orange accessions. Both the spatial and genomic distributions of somatic mutations are non-random. The spatial patterns of somatic mutations across the historic tree depend on tree growth and development and their accumulation across the tree canopy recapitulates branching topology. Analysis of the genomic distribution of somatic mutations revealed that the subtelomeres, which are large arrays of ∼180 bp repeats, are mutation hotspots. Finally, there was genomic evidence that somatic selection shapes the accumulation of somatic mutations both within the historic tree and also during clonal propagation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HYPK Interacts with COP1 and Enhances Its Activity to Repress Photomorphogenesis in Arabidopsis. 拟南芥中HYPK与COP1相互作用并增强其抑制光形态发生的活性

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-15 DOI: 10.1093/plcell/koag278

Yuan Gao, Tong Ling, Wenqiang Yang, Yanjun Jing, Rongcheng Lin

{"title":"HYPK Interacts with COP1 and Enhances Its Activity to Repress Photomorphogenesis in Arabidopsis.","authors":"Yuan Gao, Tong Ling, Wenqiang Yang, Yanjun Jing, Rongcheng Lin","doi":"10.1093/plcell/koag278","DOIUrl":"https://doi.org/10.1093/plcell/koag278","url":null,"abstract":"Plants sense light through photoreceptors to switch from skotomorphogenesis in darkness to photomorphogenesis upon light exposure. This transition is centrally governed by the CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1)/SUPPRESSOR OF PHYA-105 (SPA) E3 ubiquitin ligase complex, which targets photomorphogenesis-promoting factors like ELONGATED HYPOCOTYL5 (HY5) for degradation in darkness. This study identifies Huntingtin Yeast Partner K (HYPK) as a novel negative regulator of photomorphogenesis in Arabidopsis thaliana. HYPK functions in signaling pathways mediated by multiple photoreceptors, and its protein is stabilized by light. We demonstrate that HYPK physically interacts with the central repressor COP1 and enhances its E3 ubiquitin ligase activity towards the positive regulator HY5, thereby promoting HY5 degradation. Genetic analyses reveal that HYPK and COP1 synergistically repress photomorphogenesis largely through HY5. Furthermore, HYPK modulates the nuclear dynamics of COP1, facilitating the formation and maintenance of nuclear bodies in darkness. HYPK also regulates light-induced phytochrome B (phyB) nuclear localization and photobody organization. Moreover, HYPK differentially modulates the physical interactions of COP1 with its key binding partners phyB and SPA1. Our findings establish HYPK as a critical component that integrates photoreceptor signaling with COP1 activity by both enhancing its E3 ligase function and orchestrating the nuclear dynamics of core light signaling components.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grafting immunity: jasmonate and raffinose on the move. 嫁接免疫：茉莉酸和棉子糖在移动。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag252

Crispus M Mbaluto

引用次数: 0

Transcriptional and phytohormonal regulation of positional ear development reveals yield strategies in maize. 玉米位置穗发育的转录和植物激素调控揭示了产量策略。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag269

Lu Li, Xiaomeng Shen, Zhaobin Ren, Ziyi Yuan, Guorui Wang, Si Shen, Yuyi Zhou, Mingcai Zhang, Shuaisong Zhang, Zhaobin Dong, Feng Qin, Jinsheng Lai, Zhaohu Li, Fei Yi, Liusheng Duan

{"title":"Transcriptional and phytohormonal regulation of positional ear development reveals yield strategies in maize.","authors":"Lu Li, Xiaomeng Shen, Zhaobin Ren, Ziyi Yuan, Guorui Wang, Si Shen, Yuyi Zhou, Mingcai Zhang, Shuaisong Zhang, Zhaobin Dong, Feng Qin, Jinsheng Lai, Zhaohu Li, Fei Yi, Liusheng Duan","doi":"10.1093/plcell/koag269","DOIUrl":"10.1093/plcell/koag269","url":null,"abstract":"Maize (Zea mays L.) is a vital global crop, contributing approximately 37% of annual grain production. Enhancing yield per unit area is crucial for food security, yet research has primarily focused on single-ear traits, overlooking the regulation of double ears-a key determinant of prolificacy. While secondary ears drive yield variability under prolificacy-favoring conditions, the mechanisms governing ear formation across shoot positions remain poorly understood. Here, we performed high-resolution transcriptomic analysis of 66 samples from 3 ear types (primary, secondary, and tertiary) in maize inbred B73. We uncovered distinct hormonal developmental dynamics: strigolactone (SL) signaling genes, particularly SBP transcription factors, dominated in primary (I) ears, whereas ethylene-related genes (eg, ZmEREB131, ZmACCO35) were enriched in third (III) ears. Functional validation confirmed that knockout of ZmEREB131 and ZmACCO35 accelerated development and elongated ears compared with wild type, implicating ethylene (ETH) signaling in ear maturation arrest. Notably, SL inhibitor application synchronized primary and secondary ear development, boosting total yield by >20% without compromising primary ear performance. Our study elucidates the transcriptional networks underlying differential ear development and provides actionable strategies for yield improvement through targeted hormonal modulation. These findings advance the understanding of maize inflorescence biology and offer molecular tools for breeding high-yielding varieties.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A regulatory module integrates lipid metabolism and cell wall remodeling to establish the seed coat palisade barrier. 一个整合脂质代谢和细胞壁重塑的调节模块建立种皮栅栏屏障。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag255

Zhichao Lu, Lu Han, Zhiqun Gu, Annika Sonntag, Isabel Molina, Hongjiao Jiang, Min Wang, Xue Zhang, Hongfeng Wang, Yiteng Xu, Jing Zhang, Xu Zhang, Maofeng Chai, Zeng-Yu Wang, Kirankumar S Mysore, Jiangqi Wen, Feng Yuan, Yaling Liu, Chengchen Pan, Zhaoming Wang, Chuanen Zhou

{"title":"A regulatory module integrates lipid metabolism and cell wall remodeling to establish the seed coat palisade barrier.","authors":"Zhichao Lu, Lu Han, Zhiqun Gu, Annika Sonntag, Isabel Molina, Hongjiao Jiang, Min Wang, Xue Zhang, Hongfeng Wang, Yiteng Xu, Jing Zhang, Xu Zhang, Maofeng Chai, Zeng-Yu Wang, Kirankumar S Mysore, Jiangqi Wen, Feng Yuan, Yaling Liu, Chengchen Pan, Zhaoming Wang, Chuanen Zhou","doi":"10.1093/plcell/koag255","DOIUrl":"10.1093/plcell/koag255","url":null,"abstract":"Seed physical dormancy is a crucial adaptive trait conferred by the water-impermeable seed coat, particularly its specialized palisade layer. Although the structural components of this barrier, including the cuticle and cell wall polysaccharides, are well characterized, the molecular mechanisms coordinating their formation and remodeling remain poorly understood. Here, using single-nucleus RNA sequencing of Medicago truncatula, we identify a regulatory module essential for palisade layer formation and the establishment of physical dormancy. We show that MtKNOX4 interacts with cytoplasmic MtSAW1/2 proteins and facilitates their nuclear recruitment, thereby enabling the formation of functional nuclear MtKNOX4-MtSAW1/2 complexes. These complexes activate the expression of downstream target genes, including MtATT1 (encoding a fatty acid ω-hydroxylase belonging to the CYP86A subfamily of cytochrome P450 enzymes) and MtPAE8 (encoding a pectin acetylesterase). Thus, the MtKNOX4-MtSAW1/2 module coordinates two parallel barrier-forming pathways involving lipid polyester remodeling and pectin deacetylation, thereby reinforcing seed coat impermeability. Loss-of-function mutations in these regulators disrupt palisade layer integrity and impair physical dormancy. Collectively, our results reveal a regulatory module in which MtKNOX4-mediated nuclear recruitment of MtSAW1/2 links transcriptional regulation of lipid metabolism and cell wall remodeling to seed coat barrier formation, providing potential targets for tuning hard-seededness in legume crops.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0