Plant Cell最新文献_第4页

OsGRP3 deacetylation drives nuclear condensate assembly and transcriptional reprogramming under salt stress. 盐胁迫下OsGRP3去乙酰化驱动核凝聚物组装和转录重编程。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag254

Weiyu Xiao, Naibin Zhang, Huiping Chen, Junru Gao, Wenyao Zhang, Muhammad Qadir, Feng Yu, Long Wang

{"title":"OsGRP3 deacetylation drives nuclear condensate assembly and transcriptional reprogramming under salt stress.","authors":"Weiyu Xiao, Naibin Zhang, Huiping Chen, Junru Gao, Wenyao Zhang, Muhammad Qadir, Feng Yu, Long Wang","doi":"10.1093/plcell/koag254","DOIUrl":"10.1093/plcell/koag254","url":null,"abstract":"High soil salinity poses a major threat to global crop yields. Although cytoplasmic stress granules are known to contribute to plant responses to salt stress, the function and regulatory mechanisms of nuclear condensates in rice during stress response have remained elusive. Here, we demonstrate that the RNA-binding protein OsGRP3 assembles into nuclear condensates in response to salt stress. OsGRP3 undergoes deacetylation, which promotes its liquid-liquid phase separation and the formation of nuclear condensates. These condensates specifically recruit the OsZHD1.1 splice variant of the transcription factor OsZHD1 but not OsZHD1.2. Mechanistically, OsGRP3 enhances the transcriptional activity of OsZHD1.1, leading to the upregulation of key salt stress-responsive genes, including OsHKT1;4 and OsSOS1, ultimately limiting shoot sodium accumulation and enhancing root sodium extrusion. Our findings identify deacetylation-driven OsGRP3 condensation as a mechanism that promotes OsZHD1.1 recruitment and transcriptional activation of salt-responsive genes, thereby contributing to Na+ homeostasis and salt tolerance in rice. This study provides a mechanistic framework for understanding how stress-responsive nuclear condensates coordinate transcriptional reprogramming in plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Junction, what's your function? Mapping the exon junction complex in Arabidopsis. 结点，你的函数是什么？拟南芥外显子连接复合体的定位。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag258

Neeta Lohani, Meenu Singla-Rastogi

引用次数: 0

Specific light-harvesting complexes mediate grana stacking and prevent energy spillover between photosystems in plant chloroplasts. 在植物叶绿体中，特定的光收集复合物调节颗粒堆积并防止光系统之间的能量溢出。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag256

Zeno Guardini, Rodrigo L Gomez, Johannes Stuttmann, Parveen Akhtar, Petar H Lambrev, Luca Dall'Osto, Roberto Bassi

{"title":"Specific light-harvesting complexes mediate grana stacking and prevent energy spillover between photosystems in plant chloroplasts.","authors":"Zeno Guardini, Rodrigo L Gomez, Johannes Stuttmann, Parveen Akhtar, Petar H Lambrev, Luca Dall'Osto, Roberto Bassi","doi":"10.1093/plcell/koag256","DOIUrl":"10.1093/plcell/koag256","url":null,"abstract":"Land plant chloroplasts are characterized by a prominent morphological feature: the stacking of thylakoid membranes into grana, ie defining a continuous membranous structure surrounded by the stroma and folded into appressed grana and non-appressed domains, enclosing the thylakoid lumen. Photosystem II and its antenna system are concentrated, whereas Photosystem I and ATPase are located in the grana end membranes and, in the stroma, membranes connecting grana. Thylakoid stacking promotes the physical separation of Photosystems I and II, thereby ensuring a balanced flow of excitation energy between them. Through a genetic dissection of the light-harvesting complex subfamily Lhcb, we showed that removal of the antenna system serving Photosystem II completely abolished grana formation, whereas deletion of specific Lhcb subgroups reduced stacking by up to 40%. Lhcb5 alone was sufficient to partially restore stacking, whereas exclusive expression of Lhcb2 resulted in grana with an unusually large size, which disassembled upon illumination. Time-resolved fluorescence measurements revealed that the extent of excitation energy spillover from PSII to Photosystem I correlated with the level of Lhcb depletion, rather than with granal architecture. These findings provide genetic evidence for a functional link between Photosystem II antenna composition, lateral heterogeneity, and excitation energy partition between photosystems.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13564224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding the right balance: ASY1 recruits opposing regulators to finetune DMC1 function during meiosis. 找到正确的平衡：ASY1招募对立的调节因子来微调DMC1在减数分裂期间的功能。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag248

Julie Robinson

引用次数: 0

Natural variation in OsbHLH091 fine-tunes rice floret opening time in a JAZ-mediated dance. OsbHLH091的自然变异在jazz介导的舞蹈中微调水稻小花开放时间。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag259

Ju-Chen Chia

引用次数: 0

Best practices for biotin ligase-based proximity labeling proteomics in plant systems. 植物系统中基于生物素连接酶的接近标记蛋白质组学的最佳实践。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag257

Yiling Fang, Jacob Moe-Lange, Shou-Ling Xu, Nitzan Shabek, Yangnan Gu

{"title":"Best practices for biotin ligase-based proximity labeling proteomics in plant systems.","authors":"Yiling Fang, Jacob Moe-Lange, Shou-Ling Xu, Nitzan Shabek, Yangnan Gu","doi":"10.1093/plcell/koag257","DOIUrl":"10.1093/plcell/koag257","url":null,"abstract":"Proximity labeling (PL) proteomics, primarily powered by engineered biotin ligases such as BioID and TurboID, has emerged as a transformative approach for mapping protein association networks and subcellular proteomes in living cells. By covalently biotinylating proteins within a nanometer-scale radius of a bait protein, PL captures transient, weak, and spatially restricted associations that often escape conventional affinity-based methods. However, applying PL in plants introduces distinctive challenges, including rigid cell walls that can limit biotin penetration, tissue-specific variation in substrate accessibility, enzyme temperature sensitivity, and the difficulty of removing excess free biotin after labeling. Here, we outline best practices for designing and implementing biotin ligase-based PL experiments in plant systems, drawing on experience across multiple species. We discuss key considerations, including enzyme selection, expression system design, fusion protein validation, biotin delivery strategies, protein extraction and enrichment, and mass spectrometry-based analysis. We also highlight emerging quantitative and conditional PL approaches that enable dynamic comparison of proxiomes across developmental stages, environmental conditions, and genetic backgrounds. Throughout, we emphasize the importance of rigorous controls, careful terminology, and orthogonal validation to ensure biologically meaningful interpretation of PL datasets. These guidelines aim to standardize experimental design and interpretation, facilitating reproducible and biologically meaningful PL studies in plant systems.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the repertoire of microRNA target mimicry in plants. 扩大植物中microRNA靶标模仿的范围。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag265

Youhong Fan, Guodong Ren

{"title":"Expanding the repertoire of microRNA target mimicry in plants.","authors":"Youhong Fan, Guodong Ren","doi":"10.1093/plcell/koag265","DOIUrl":"10.1093/plcell/koag265","url":null,"abstract":"microRNAs (miRNAs) are master regulators of gene expression, guiding ARGONAUTE proteins to bind to and repress target RNAs. Interestingly, a special class of target RNAs, termed target mimics (TMs), can in turn trigger miRNA degradation in plants-a process genetically dependent on the F-box protein HAWAIIAN SKIRT (HWS). However, the pairing rules governing effective TM sites remain unclear. Here, we systematically investigate the pairing architectures that enable plant TM-directed miRNA degradation. Using transient expression in Nicotiana benthamiana leaves and validation in stable Arabidopsis thaliana transgenic lines, we demonstrate that effective TM sites contain a central or near-central unpaired region-classified as insertions (I-type), mismatches (X-type), or deletions (D-type)-flanked by complementary segments. I-type sites tolerate considerable variation in bulge size and position, whereas X- and D-types are more constrained. By incorporating these features into a predictive pipeline, we identified endogenous TMs with alternative pairing architectures, including EARLY NODULIN-LIKE PROTEIN5 (ENODL5), which fine-tunes miR159 levels during Arabidopsis floral development in an HWS-dependent manner. Duplex stability and non-coding context also contribute to TM efficacy. Together, our findings establish a robust empirical framework for understanding, designing, and predicting miRNA TMs in plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoperiod and UV-B orchestrate ICR1 to control seed isoflavonoid accumulation in soybean. 光周期和UV-B调控ICR1调控大豆种子中异黄酮的积累。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag268

Mingyang Jian, Huan Wu, Cuirong Tan, Yushuang Yao, Shu Liu, Yilong Hu, Xingliang Hou, Chunyu Zhang

{"title":"Photoperiod and UV-B orchestrate ICR1 to control seed isoflavonoid accumulation in soybean.","authors":"Mingyang Jian, Huan Wu, Cuirong Tan, Yushuang Yao, Shu Liu, Yilong Hu, Xingliang Hou, Chunyu Zhang","doi":"10.1093/plcell/koag268","DOIUrl":"10.1093/plcell/koag268","url":null,"abstract":"Being sessile, plants optimize their physiological and metabolic processes in response to ambient environmental cues, such as light. However, the molecular mechanisms underlying environmental regulation of seed isoflavonoid biosynthesis in soybean remain largely elusive. Here, our genome-wide association study identifies isoflavone content regulator 1 (ICR1), encoding a regulator of chromosome condensation (RCC1) family protein, as a positive regulator of seed isoflavonoid accumulation in soybean. The CONSTANS (CO) homolog GmCOL2b directly suppresses ICR1 transcription by binding to a CORE cis-element. Notably, natural variations flanking the CORE sequence influence GmCOL2b binding affinity, thereby modulating ICR1 transcription and seed isoflavone content. We further demonstrate that photoperiod significantly affects seed isoflavone content, with short-day conditions promoting isoflavonoid accumulation by relieving GmCOL2b-mediated repression of ICR1, while UV-B radiation facilitates ICR1 protein accumulation. Subsequently, ICR1 interacts with GmMYB12B2 to enhance seed isoflavonoid biosynthesis. Our findings elucidate how soybean integrates environmental light signals, including photoperiod and UV-B signaling that vary across seasons or latitudes, to coordinate seed isoflavonoid biosynthesis, providing a valuable genetic resource for improving soybean nutritional quality.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148907091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsWOX13: a molecular bridge linking Ehd1 to florigen Hd3a in regulating rice heading date. OsWOX13：连接Ehd1和花原Hd3a调控水稻抽穗期的分子桥。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag270

Hongwei Jing

引用次数: 0

A cryptic START domain regulates deeply conserved transcription factors. 一个神秘的START结构域调节深度保守的转录因子。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-09-01 DOI: 10.1093/plcell/koag267

Courtney E Dresden, Ekaterina P Andrianova, Brian J Smith, Nicole I Callery, Dominic Kolonay, Ashton S Holub, Ricardo A Urquidi Camacho, Sarah G Choudury, Isabella J Higgins, Igor B Zhulin, Aman Y Husbands

{"title":"A cryptic START domain regulates deeply conserved transcription factors.","authors":"Courtney E Dresden, Ekaterina P Andrianova, Brian J Smith, Nicole I Callery, Dominic Kolonay, Ashton S Holub, Ricardo A Urquidi Camacho, Sarah G Choudury, Isabella J Higgins, Igor B Zhulin, Aman Y Husbands","doi":"10.1093/plcell/koag267","DOIUrl":"10.1093/plcell/koag267","url":null,"abstract":"Transcription factors (TFs) integrate a diverse array of inputs to achieve the exquisite control of gene expression necessary for life. In plants, this is exemplified by the deeply conserved CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-Zip III) family of TFs. HD-Zip III activity is controlled by inputs at transcriptional, post-transcriptional, and post-translational levels. As part of their multidomain architecture, HD-Zip III TFs contain a StAR-related lipid transfer (START) domain, a ubiquitously distributed evolutionary module that binds various types of lipophilic ligands. Here, we show that HD-Zip III and HD-Zip IV proteins contain an additional cryptic, deeply conserved START domain which we term the disorder-containing START domain (dSTART). The dSTART domain is required for HD-Zip III developmental function, promoting homodimerization and controlling subcellular localization and DNA-binding competence. The dSTART domain also helps discriminate responsive from nonresponsive binding sites across the HD-Zip III shared genetic network. Finally, we identify candidate ligands of the dSTART domain including several species of phosphatidylglycerol. The identification and functional characterization of a cryptic START domain provides new mechanistic insights into a deeply conserved family of TFs with roles in nearly all aspects of plant development.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148901039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0