Plant Cell最新文献_第5页

UnERFing auxin-mediated degradation in the emerging lateral root. 在萌发的侧根中解除辅助素介导的降解。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae079

Rory Osborne

引用次数: 0

Reply: Does the polyubiquitination pathway operate inside intact chloroplasts to remove proteins? 回复：多泛素化途径是否在完整叶绿体内运行以清除蛋白质？

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae105

R Paul Jarvis, Jialong Li, Rongcheng Lin, Qihua Ling, Yuping Lyu, Yi Sun, Zujie Yao

引用次数: 0

Does the polyubiquitination pathway operate inside intact chloroplasts to remove proteins? 多泛素化途径是否在完整叶绿体内运行以清除蛋白质？

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae104

Klaas J van Wijk, Zach Adam

引用次数: 0

The brassinosteroid receptor StBRI1 promotes tuber development by enhancing plasma membrane H+-ATPase activity in potato. 芸苔素类固醇受体 StBRI1 通过增强马铃薯质膜 H+-ATP 酶活性促进块茎发育

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae163

Rui Deng, Shuhua Huang, Jia Du, Dan Luo, Jianwei Liu, Yan Zhao, Chongyang Zheng, Tiantian Lei, Qi Li, Siwei Zhang, Meng Jiang, Tong Jin, Dehai Liu, Shufen Wang, Yanfeng Zhang, Xiaofeng Wang

{"title":"The brassinosteroid receptor StBRI1 promotes tuber development by enhancing plasma membrane H+-ATPase activity in potato.","authors":"Rui Deng, Shuhua Huang, Jia Du, Dan Luo, Jianwei Liu, Yan Zhao, Chongyang Zheng, Tiantian Lei, Qi Li, Siwei Zhang, Meng Jiang, Tong Jin, Dehai Liu, Shufen Wang, Yanfeng Zhang, Xiaofeng Wang","doi":"10.1093/plcell/koae163","DOIUrl":"10.1093/plcell/koae163","url":null,"abstract":"The brassinosteroid (BR) receptor BRASSINOSTEROID-INSENSITIVE 1 (BRI1) plays a critical role in plant growth and development. Although much is known about how BR signaling regulates growth and development in many crop species, the role of StBRI1 in regulating potato (Solanum tuberosum) tuber development is not well understood. To address this question, a series of comprehensive genetic and biochemical methods were applied in this investigation. It was determined that StBRI1 and Solanum tuberosum PLASMA MEMBRANE (PM) PROTON ATPASE2 (PHA2), a PM-localized proton ATPase, play important roles in potato tuber development. The individual overexpression of StBRI1 and PHA2 led to a 22% and 25% increase in tuber yield per plant, respectively. Consistent with the genetic evidence, in vivo interaction analysis using double transgenic lines and PM H+-ATPase activity assays indicated that StBRI1 interacts with the C-terminus of PHA2, which restrains the intramolecular interaction of the PHA2 C-terminus with the PHA2 central loop to attenuate autoinhibition of PM H+-ATPase activity, resulting in increased PHA2 activity. Furthermore, the extent of PM H+-ATPase autoinhibition involving phosphorylation-dependent mechanisms corresponds to phosphorylation of the penultimate Thr residue (Thr-951) in PHA2. These results suggest that StBRI1 phosphorylates PHA2 and enhances its activity, which subsequently promotes tuber development. Altogether, our results uncover a BR-StBRI1-PHA2 module that regulates tuber development and suggest a prospective strategy for improving tuberous crop growth and increasing yield via the cell surface-based BR signaling pathway.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141180554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A viral small interfering RNA-host plant mRNA pathway modulates virus-induced drought tolerance by enhancing autophagy. 病毒小干扰 RNA-寄主植物 mRNA 通路通过增强自噬调节病毒诱导的耐旱性。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae158

Xinyang Wu, Shuting Chen, Zixin Zhang, Weixin Zhou, Ting Sun, Kang Ning, Min Xu, Xubo Ke, Pei Xu

{"title":"A viral small interfering RNA-host plant mRNA pathway modulates virus-induced drought tolerance by enhancing autophagy.","authors":"Xinyang Wu, Shuting Chen, Zixin Zhang, Weixin Zhou, Ting Sun, Kang Ning, Min Xu, Xubo Ke, Pei Xu","doi":"10.1093/plcell/koae158","DOIUrl":"10.1093/plcell/koae158","url":null,"abstract":"Virus-induced drought tolerance presents a fascinating facet of biotic-abiotic interaction in plants, yet its molecular intricacies remain unclear. Our study shows that cowpea mild mottle virus (CPMMV) infection enhances drought tolerance in common bean (Phaseolus vulgaris) plants through a virus-derived small interfering RNA (vsiRNA)-activated autophagy pathway. Specifically, a 21 nt vsiRNA originating from the CPMMV Triple Gene Block1 (TGB1) gene targeted the 5' untranslated region (UTR) of the host Teosinte branched 1, Cycloidea, Proliferating Cell Factor (TCP) transcription factor gene PvTCP2, independent of the known role of TGB1 as an RNA silencing suppressor. This targeting attenuated the expression of PvTCP2, which encodes a transcriptional repressor, and in turn upregulated the core autophagy-related gene (ATG) PvATG8c, leading to activated autophagy activity surpassing the level induced by drought or CPMMV infection alone. The downstream EARLY RESPONSIVE TO DEHYDRATION (ERD) effector PvERD15 is a homologue of Arabidopsis thaliana AtERD15, which positively regulates stomatal aperture. PvERD15 was degraded in PvATG8c-mediated autophagy. Therefore, we establish a TGB1-PvTCP2-PvATG8c-PvERD15 module as a trans-kingdom fine-tuning mechanism that contributes to virus-induced drought tolerance in plant-drought-virus interactions.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors. 生物工程分泌蛋白酶将不同的 Rcr3 直向同源物和旁系同源物转化为细胞外免疫共受体。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae183

Jiorgos Kourelis, Mariana Schuster, Fatih Demir, Oliver Mattinson, Sonja Krauter, Parvinderdeep S Kahlon, Ruby O'Grady, Samantha Royston, Ana Lucía Bravo-Cazar, Brian C Mooney, Pitter F Huesgen, Sophien Kamoun, Renier A L van der Hoorn

{"title":"Bioengineering secreted proteases converts divergent Rcr3 orthologs and paralogs into extracellular immune co-receptors.","authors":"Jiorgos Kourelis, Mariana Schuster, Fatih Demir, Oliver Mattinson, Sonja Krauter, Parvinderdeep S Kahlon, Ruby O'Grady, Samantha Royston, Ana Lucía Bravo-Cazar, Brian C Mooney, Pitter F Huesgen, Sophien Kamoun, Renier A L van der Hoorn","doi":"10.1093/plcell/koae183","DOIUrl":"10.1093/plcell/koae183","url":null,"abstract":"Secreted immune proteases \"Required for Cladosporium resistance-3\" (Rcr3) and \"Phytophthora-inhibited protease-1\" (Pip1) of tomato (Solanum lycopersicum) are both inhibited by Avirulence-2 (Avr2) from the fungal plant pathogen Cladosporium fulvum. However, only Rcr3 acts as a decoy co-receptor that detects Avr2 in the presence of the Cf-2 immune receptor. Here, we identified crucial residues in tomato Rcr3 that are required for Cf-2-mediated signaling and bioengineered various proteases to trigger Avr2/Cf-2-dependent immunity. Despite substantial divergence in Rcr3 orthologs from eggplant (Solanum melongena) and tobacco (Nicotiana spp.), minimal alterations were sufficient to trigger Avr2/Cf-2-mediated immune signaling. By contrast, tomato Pip1 was bioengineered with 16 Rcr3-specific residues to initiate Avr2/Cf-2-triggered immune signaling. These residues cluster on one side of the protein next to the substrate-binding groove, indicating a potential Cf-2 interaction site. Our findings also revealed that Rcr3 and Pip1 have distinct substrate preferences determined by two variant residues and that both are suboptimal for binding Avr2. This study advances our understanding of Avr2 perception and opens avenues to bioengineer proteases to broaden pathogen recognition in other crops.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation. 绘制植物 SUMO 修饰系统的进化路径图，揭示发育对胁迫适应的分子硬连接。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae192

Srayan Ghosh, Macarena Mellado Sanchez, Kawinnat Sue-Ob, Dipan Roy, Andrew Jones, Miguel A Blazquez, Ari Sadanandom

{"title":"Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation.","authors":"Srayan Ghosh, Macarena Mellado Sanchez, Kawinnat Sue-Ob, Dipan Roy, Andrew Jones, Miguel A Blazquez, Ari Sadanandom","doi":"10.1093/plcell/koae192","DOIUrl":"10.1093/plcell/koae192","url":null,"abstract":"SUMO modification is part of the spectrum of Ubiquitin-like (UBL) systems that give rise to proteoform complexity through post-translational modifications (PTMs). Proteoforms are essential modifiers of cell signaling for plant adaptation to changing environments. Exploration of the evolutionary emergence of Ubiquitin-like (UBL) systems unveils their origin from prokaryotes, where it is linked to the mechanisms that enable sulfur uptake into biomolecules. We explore the emergence of the SUMO machinery across the plant lineage from single-cell to land plants. We reveal the evolutionary point at which plants acquired the ability to form SUMO chains through the emergence of SUMO E4 ligases, hinting at its role in facilitating multicellularity. Additionally, we explore the possible mechanism for the neofunctionalization of SUMO proteases through the fusion of conserved catalytic domains with divergent sequences. We highlight the pivotal role of SUMO proteases in plant development and adaptation, offering new insights into target specificity mechanisms of SUMO modification during plant evolution. Correlating the emergence of adaptive traits in the plant lineage with established experimental evidence for SUMO in developmental processes, we propose that SUMO modification has evolved to link developmental processes to adaptive functions in land plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What doesn't kill you makes you stronger: Multi-omics analysis of jasmonate responses in rice. 杀不死你的东西会让你更强大：水稻茉莉酸反应的多组学分析。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae188

Raul Sanchez-Muñoz

引用次数: 0

Are two stresses better than one? In-depth analysis of 5 Arabidopsis accessions under water and nitrogen stress. 两种胁迫优于一种胁迫吗？深入分析拟南芥五个品种在水和氮胁迫下的表现。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae174

Shanice S Webster

引用次数: 0

Focus on proteolysis. 关注蛋白质分解。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-03 DOI: 10.1093/plcell/koae182

Nancy A Eckardt, Pascal Genschik, Liwen Jiang, Xin Li, Marisa S Otegui, Ari Sadanandom, Steven H Spoel, Klaas J van Wijk, Dolf Weijers

引用次数: 0