Plant Cell最新文献_第6页

Single-cell multiomic characterization of the soybean root response to cyst nematode infection. 大豆根系对包囊线虫感染反应的单细胞多组学特征。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag232

Xuan Zhang, Vinavi A Gamage, Xunliang Liu, Ziliang Luo, Mark A A Minow, Hao Zhang, Melissa G Mitchum, Robert J Schmitz

{"title":"Single-cell multiomic characterization of the soybean root response to cyst nematode infection.","authors":"Xuan Zhang, Vinavi A Gamage, Xunliang Liu, Ziliang Luo, Mark A A Minow, Hao Zhang, Melissa G Mitchum, Robert J Schmitz","doi":"10.1093/plcell/koag232","DOIUrl":"10.1093/plcell/koag232","url":null,"abstract":"Soybean cyst nematodes parasitize soybean roots by inducing the formation of multinucleate syncytia to feed and complete their life cycle. However, the molecular basis of syncytia initiation and development remains limited. Here, we generated an integrated single-nucleus RNA and chromatin accessibility profile of soybean roots from infected and uninfected plants. We profiled 56,448 high-quality nuclei and identified all major root cell types, including distinct subpopulations likely enriched with syncytial nuclei. Transcriptomic and chromatin accessibility analyses support their procambium cell signature that progresses through distinct stages associated with immune suppression, cell fusion, and endoreduplication. Integrative gene expression and transcription factor motif chromatin accessibility analyses predicted Calmodulin-binding transcription activator 1 as a potential transcriptional repressor of defense-related genes, including receptor-like kinases, whereas MYB, MYB-related, and E2F likely act as potential transcriptional activators that coordinate cell wall remodeling, chromatin modification, and DNA replication, respectively, across developmental trajectories. These findings provide areas to pursue in the future to understand mechanistic insights into host reprogramming during nematode parasitism and serve as a foundational resource for engineering nematode-resistant soybean.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148654202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sub-resolution imaging and Bayesian analysis of exocytosis events reveals nanoscale patterning by cortical microtubule arrays. 胞吐事件的亚分辨率成像和贝叶斯分析揭示了皮层微管阵列的纳米级模式。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag222

Jelmer J Lindeboom, Ryan Gutierrez, Viktor Kirik, David W Ehrhardt

{"title":"Sub-resolution imaging and Bayesian analysis of exocytosis events reveals nanoscale patterning by cortical microtubule arrays.","authors":"Jelmer J Lindeboom, Ryan Gutierrez, Viktor Kirik, David W Ehrhardt","doi":"10.1093/plcell/koag222","DOIUrl":"10.1093/plcell/koag222","url":null,"abstract":"The microtubule cytoskeleton organizes exocytosis to enable cellular morphogenesis, but how non-centrosomal arrays control exocytic site positioning remains poorly understood. Using elongating Arabidopsis thaliana cells as a model, we developed quantitative methods to move beyond coarse correlation and reveal the precise spatial relationship between cortical microtubules and secretion. We identify KEULE, an essential SEC/MUNC protein, as a dynamic exocytosis marker that forms clusters with stereotyped assembly and disassembly kinetics at discrete secretion sites. Combining confocal microscopy with super-resolution analysis and Bayesian inference, we quantified microtubule-exocytosis positioning at nanometer precision. This analysis revealed that microtubules create ∼180-nm enrichment zones flanked by ∼520-nm depletion zones, generating a spatial pattern that replicates the cortical array structure. Unexpectedly, Bayesian inference showed strong evidence for a flat enrichment profile within these zones rather than peaked distributions. This flat profile, combined with the ∼180-nm width, challenges a direct vesicle capture mechanism, suggesting consideration of alternative models such as a mechanism where microtubules organize the local membrane environment to create preferred exocytosis territories. These findings establish quantitative spatial rules for how non-centrosomal microtubule arrays organize secretion.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 8","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13594665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148819261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auxin and ABA join forces through bZIP23 to promote rice seed vigor. 生长素和ABA通过bZIP23共同促进水稻种子活力。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag240

Jiajun Wang, Wenjing Liu

引用次数: 0

Metabolic control of cellular reprogramming: phospholipid remodeling tunes auxin signaling. 细胞重编程的代谢控制：磷脂重塑调节生长素信号。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag239

Jiajun Wang, Dandan Hu

引用次数: 0

Breaking down the wall: a noncoding RNA network regulates pectin remodeling during tomato ripening. 破壁：一个非编码RNA网络调节番茄成熟过程中的果胶重塑。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag230

Soledad Traubenik

引用次数: 0

The miPEP171b-miR171b-SCL15/HDA19 module regulates histone deacetylation to confer cold tolerance in grapevine. miPEP171b-miR171b-SCL15/HDA19模块调节组蛋白去乙酰化，赋予葡萄的耐寒性。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag236

Dongying Fan, Junpeng Li, Lujia Wang, Zhen Zhang, Yuanyuan Xu, Yue Song, Yuanxu Teng, Chenlu Du, Lipeng Zhang, Juan He, Yi Ren, Chao Ma

{"title":"The miPEP171b-miR171b-SCL15/HDA19 module regulates histone deacetylation to confer cold tolerance in grapevine.","authors":"Dongying Fan, Junpeng Li, Lujia Wang, Zhen Zhang, Yuanyuan Xu, Yue Song, Yuanxu Teng, Chenlu Du, Lipeng Zhang, Juan He, Yi Ren, Chao Ma","doi":"10.1093/plcell/koag236","DOIUrl":"10.1093/plcell/koag236","url":null,"abstract":"Cold stress is a major environmental factor limiting the cultivation and productivity of grapevine (Vitis vinifera L.). While microRNAs (miRNAs) are well-established post-transcriptional regulators, the functional roles of microRNA-encoded peptides (miPEPs) in cold adaptation remain poorly understood. Through quantitative proteomics, we identified miPEP171b, encoded within the pri-miR171b transcript, as a key regulator of the grapevine cold stress response. Cold stress induces pri-miR171b expression, significantly upregulating both miPEP171b and pre-miR171b. As a small peptide, miPEP171b further promotes the accumulation of vvi-miR171b. Subsequent investigations reveal that miPEP171b and vvi-miR171b enhance cold tolerance by suppressing their target gene, VvSCL15, which acts as a negative regulator of the cold stress response. Under normal conditions, VvSCL15 recruits the histone deacetylase VvHDA19 to form a regulatory complex, which maintains low histone H3 acetylation levels and suppresses the expression of key cold-responsive genes, such as VvCOR27, VvCAM-1, and VvCML46. Upon cold exposure, the accumulation of miPEP171b and vvi-miR171b suppresses the expression of VvSCL15, dismantling this repressor complex and initiating extensive transcriptional reprogramming. Metabolomic analysis further demonstrates that miPEP171b orchestrates adaptive shifts in amino acid and sugar metabolism. Collectively, our findings define a novel miPEP171b-miR171b-SCL15/HDA19 module that integrates peptide signaling with chromatin remodeling to confer cold tolerance in grapevine. This study uncovers a previously unrecognized epigenetic switch for cold adaptation and provides a strategic target for enhancing environmental resilience in horticultural crops.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148689487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of progenitor genomes offers insights to reduce deleterious burden in hybrid potato breeding. 祖先基因组的分析为减轻杂交马铃薯育种中的有害负担提供了新的思路。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag227

Weizhi Liu, Yong Hu, Zihui Ding, Qing Yu, Haixu Peng, Xianyi Zhang, Xinyao Zhao, Yao Zhou, Jingjing Zhai, Yongfeng Zhou, Guillaume P Ramstein, Sanwen Huang, Yaoyao Wu

{"title":"Analysis of progenitor genomes offers insights to reduce deleterious burden in hybrid potato breeding.","authors":"Weizhi Liu, Yong Hu, Zihui Ding, Qing Yu, Haixu Peng, Xianyi Zhang, Xinyao Zhao, Yao Zhou, Jingjing Zhai, Yongfeng Zhou, Guillaume P Ramstein, Sanwen Huang, Yaoyao Wu","doi":"10.1093/plcell/koag227","DOIUrl":"10.1093/plcell/koag227","url":null,"abstract":"Hybrid potato breeding aims to transition potato from clonal tetraploid to seed-propagated diploid. However, this process is hindered by historically accumulated deleterious variants, resulting in weak field performance of existing homozygous inbreds and compromised heterosis in hybrids. To accelerate genome design for hybrid potato breeding, it is essential to understand how deleterious variation was reshaped during domestication and whether this knowledge can guide purging of deleterious variants. To address this, we constructed a genome-wide deleterious variant map spanning 502 accessions, including both progenitor and landrace materials. Compared with the landrace panel, the progenitor panel displayed higher genetic diversity, longer genetic distance, and a lower frequency of deleterious variants, properties expected to enhance heterosis and guide the purging of deleterious variants. Moreover, deleterious variants, including those subject to turnover accumulation and even fixation during domestication, were preferentially enriched in selective sweeps in the landrace panel. All these illustrated a clear \"hidden cost\" of domestication. Consistent with this, deleterious variants in potato hybrid and their landrace-derived inbred parents increased about 2-fold in allele frequency during domestication, pointing to the potential of Solanum candolleanum accessions as donors for inbreds improvement. We predicted a progenitor-assisted genome with about half the deleterious burden of inbreds by leveraging the deleterious map of the Solanum candolleanum progenitor, providing a predictive genomic framework for guiding future purging of deleterious variants during inbred improvement. Our work bridges domestication genomics with genome-design breeding and shifts the use of wild progenitors from single-gene introgression toward genome-wide optimization.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HHO5 balances the plant nitrogen diet. HHO5平衡植物的氮饮食。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag241

Luciano M Di Fino

引用次数: 0

Transient abRNA and steady-state mRNA levels set the thresholds for sense PTGS initiation and amplification. 瞬时abRNA和稳态mRNA水平设置了Sense PTGS启动和扩增的阈值。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag245

Hongwei Jing

引用次数: 0

Green flower coloration through AT-hook protein-mediated histone H3K27ac deacetylation in chrysanthemum. 通过AT-hook蛋白介导的组蛋白H3K27ac去乙酰化使菊花呈现绿色。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag225

Hongfeng Huang, Jiaqi Wen, Ying Zheng, Dan Li, Mengru Li, Yuan Liu, Ning Zhang, Wenyuan Wang, Yanjie Xu, Xin Zhao, Junping Gao, Bo Hong, Zhaoyu Gu

{"title":"Green flower coloration through AT-hook protein-mediated histone H3K27ac deacetylation in chrysanthemum.","authors":"Hongfeng Huang, Jiaqi Wen, Ying Zheng, Dan Li, Mengru Li, Yuan Liu, Ning Zhang, Wenyuan Wang, Yanjie Xu, Xin Zhao, Junping Gao, Bo Hong, Zhaoyu Gu","doi":"10.1093/plcell/koag225","DOIUrl":"10.1093/plcell/koag225","url":null,"abstract":"Chloroplast development and photosynthetic pigment synthesis in leaves are governed by a highly conserved Golden2-like (GLK) transcription factor network. However, the mechanisms sustaining green pigmentation in non-foliar organs such as flowers remain unclear. Here, we show that floral pigmentation is controlled by a GLK-independent pathway, identifying the AT-hook protein CmAHL8L as a central regulator of green coloration in chrysanthemum. Silencing CmAHL8L reduced green pigmentation in ray florets, accompanied by decreased chlorophyll and carotenoid levels. CmAHL8L promotes chlorophyll accumulation by repressing the transcriptional repressor CmAHL23, thereby derepressing GLUTAMYL-tRNA REDUCTASE 1 (CmHEMA1), while concurrently preventing carotenoid degradation through direct inhibition of CAROTENOID CLEAVAGE DIOXYGENASE (CmCCD4a). Mechanistically, CmAHL8L recruits the histone deacetylase CmHDT1 to remove H3K27ac marks at the CmAHL23 and CmCCD4a promoters, suppressing their transcription. We further demonstrate that this AHL8L-HDT1 complex is conserved across diverse species. Collectively, our work defines a predominant regulatory mechanism in photosynthetic pigments of the flower, distinct from leaf mechanisms, providing key genetic insights into the regulation and evolution of flower coloration.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148621174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0