Plant Cell最新文献_第7页

Conserved cis-elements enable NODULES WITH ACTIVATED DEFENSE1 regulation by NODULE INCEPTION during nodulation. 保守的顺式元件使 NODULES WITH ACTIVATED DEFENSE1 能够在拔节过程中通过 NODULE INCEPTION 进行调控。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae229

Haixiang Yu, Aifang Xiao, Zhongmin Zou, Qiujin Wu, Lin Chen, Dandan Zhang, Yuzhang Sun, Chao Wang, Jianbo Cao, Hui Zhu, Zhongming Zhang, Yangrong Cao

{"title":"Conserved cis-elements enable NODULES WITH ACTIVATED DEFENSE1 regulation by NODULE INCEPTION during nodulation.","authors":"Haixiang Yu, Aifang Xiao, Zhongmin Zou, Qiujin Wu, Lin Chen, Dandan Zhang, Yuzhang Sun, Chao Wang, Jianbo Cao, Hui Zhu, Zhongming Zhang, Yangrong Cao","doi":"10.1093/plcell/koae229","DOIUrl":"10.1093/plcell/koae229","url":null,"abstract":"Symbiotic nitrogen fixation within nitrogen-fixing clade (NFC) plants is thought to have arisen from a single gain followed by massive losses in the genomes of ancestral non-nodulating plants. However, molecular evidence supporting this model is limited. Here, we confirm through bioinformatic analysis that NODULES WITH ACTIVATED DEFENSE1 (NAD1) is present only in NFC plants and is thus an NFC-specific gene. Moreover, NAD1 was specifically expressed in nodules. We identified three conserved nodulation-associated cis-regulatory elements (NACE1-3) in the promoter of LjNAD1 from Lotus japonicus that are required for its nodule specific expression. A survey of NFC plants revealed that NACE1 and NACE2 are specific to the Fabales and Papilionoideae, respectively, while NACE3 is present in all NFC plants. Moreover, we found that nodule inception (NIN) directly binds to all three NACEs to activate NAD1 expression. Mutation of L. japonicus LjNAD1 resulted in the formation of abnormal symbiosomes with enlarged symbiosome space and frequent breakdown of bacteroids in nodules, resembling phenotypes reported for Medicago truncatula Mtnad1 and Mtnin mutants. These data point to NIN-NAD1 as an important module regulating rhizobial accommodation in nodules. The regulation of NAD1 by NIN in the NFC ancestor represent an important evolutionary adaptation for nodulation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11448908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits. SEPALLATA 2 中的单核苷酸多态性是葫芦果实长度变异的基础。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae228

Weiyuan Song, Yang Xie, Bin Liu, Yuxiang Huang, Zhihua Cheng, Zilong Zhao, Di Tian, Yan Geng, Jingyu Guo, Chuang Li, Daixi She, Yanting Zhong, Min Li, Liu Liu, Jiacai Chen, Chengzhen Sun, Xuejun Zhang, Zhaoyang Zhou, Jinsheng Lai, Ming Xin, Liying Yan, Jianyu Zhao, Xiaolan Zhang

{"title":"Single nucleotide polymorphisms in SEPALLATA 2 underlie fruit length variation in cucurbits.","authors":"Weiyuan Song, Yang Xie, Bin Liu, Yuxiang Huang, Zhihua Cheng, Zilong Zhao, Di Tian, Yan Geng, Jingyu Guo, Chuang Li, Daixi She, Yanting Zhong, Min Li, Liu Liu, Jiacai Chen, Chengzhen Sun, Xuejun Zhang, Zhaoyang Zhou, Jinsheng Lai, Ming Xin, Liying Yan, Jianyu Zhao, Xiaolan Zhang","doi":"10.1093/plcell/koae228","DOIUrl":"10.1093/plcell/koae228","url":null,"abstract":"Complete disruption of critical genes is generally accompanied by severe growth and developmental defects, which dramatically hinder its utilization in crop breeding. Identifying subtle changes, such as single-nucleotide polymorphisms (SNPs), in critical genes that specifically modulate a favorable trait is a prerequisite to fulfill breeding potential. Here, we found 2 SNPs in the E-class floral organ identity gene cucumber (Cucumis sativus) SEPALLATA2 (CsSEP2) that specifically regulate fruit length. Haplotype (HAP) 1 (8G2667A) and HAP2 (8G2667T) exist in natural populations, whereas HAP3 (8A2667T) is induced by ethyl methanesulfonate mutagenesis. Phenotypic characterization of 4 near-isogenic lines and a mutant line showed that HAP2 fruits are significantly longer than those of HAP1, and those of HAP3 are 37.8% longer than HAP2 fruit. The increasing fruit length in HAP1-3 was caused by a decreasing inhibitory effect on CRABS CLAW (CsCRC) transcription (a reported positive regulator of fruit length), resulting in enhanced cell expansion. Moreover, a 7638G/A-SNP in melon (Cucumis melo) CmSEP2 modulates fruit length in a natural melon population via the conserved SEP2-CRC module. Our findings provide a strategy for utilizing essential regulators with pleiotropic effects during crop breeding.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11448892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the role of flavonoids in ROS management during heat stress in tomato pollen. 解码类黄酮在番茄花粉热应激过程中的 ROS 管理中的作用。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae234

Nitin Uttam Kamble

引用次数: 0

New insights into alternative splicing in rice using population-level transcriptomics. 利用群体水平的转录组学深入了解水稻的替代剪接。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae214

Nicolas M Doll

引用次数: 0

Seeing hormones in action: High-resolution gibberellin dynamics in nodules. 观察激素的作用：结核中赤霉素的高分辨率动态变化。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae216

Min-Yao Jhu, Thomas B Irving

引用次数: 0

The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage. 转录因子ZAT5和BLH2/4调控拟南芥种皮粘液中的高半乳糖醛酸脱甲酯化。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae209

Minmin Xie, Anming Ding, Yongfeng Guo, Jinhao Sun, Wanya Qiu, Mingli Chen, Zhiyuan Li, Shanshan Li, Gongke Zhou, Yan Xu, Meng Wang, Aurore Richel, Daping Gong, Yingzhen Kong

{"title":"The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage.","authors":"Minmin Xie, Anming Ding, Yongfeng Guo, Jinhao Sun, Wanya Qiu, Mingli Chen, Zhiyuan Li, Shanshan Li, Gongke Zhou, Yan Xu, Meng Wang, Aurore Richel, Daping Gong, Yingzhen Kong","doi":"10.1093/plcell/koae209","DOIUrl":"10.1093/plcell/koae209","url":null,"abstract":"The level of methylesterification alters the functional properties of pectin, which is believed to influence plant growth and development. However, the mechanisms that regulate demethylesterification remain largely unexplored. Pectin with a high degree of methylesterification is produced in the Golgi apparatus and then transferred to the primary cell wall where it is partially demethylesterified by pectin methylesterases (PMEs). Here, we show that in Arabidopsis (Arabidopsis thaliana) seed mucilage, pectin demethylesterification is negatively regulated by the transcription factor ZINC FINGER FAMILY PROTEIN5 (ZAT5). Plants carrying null mutations in ZAT5 had increased PME activity, decreased pectin methylesterification, and produced seeds with a thinner mucilage layer. We provide evidence that ZAT5 binds to a TGATCA motif and thereby negatively regulates methylesterification by reducing the expression of PME5, HIGHLY METHYL ESTERIFIED SEEDS (HMS)/PME6, PME12, and PME16. We also demonstrate that ZAT5 physically interacts with BEL1-LIKE HOMEODOMAIN2 (BLH2) and BLH4 transcription factors. BLH2 and BLH4 are known to modulate pectin demethylesterification by directly regulating PME58 expression. The ZAT5-BLH2/4 interaction provides a mechanism to control the degree of pectin methylesterification in seed coat mucilage by modifying each transcription factor's ability to regulate the expression of target genes encoding PMEs. Taken together, these findings reveal a transcriptional regulatory module comprising ZAT5, BLH2, and BLH4, that functions in modulating the demethylesterification of homogalacturonan in seed coat mucilage.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the archives: A plant immune hub before, after, and way after its discovery. 来自档案：植物免疫中枢发现前、发现后和发现后的漫长岁月

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae205

Bradley Laflamme

引用次数: 0

Dual roles of the MPK3 and MPK6 mitogen-activated protein kinases in regulating Arabidopsis stomatal development. MPK3和MPK6丝裂原活化蛋白激酶在调节拟南芥气孔发育中的双重作用

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae225

Mengyun Wu, Shiyuan Wang, Panpan Ma, Bixin Li, Huiqing Hu, Ziling Wang, Qin Qiu, Yujie Qiao, Dongdong Niu, Wolfgang Lukowitz, Shuqun Zhang, Mengmeng Zhang

{"title":"Dual roles of the MPK3 and MPK6 mitogen-activated protein kinases in regulating Arabidopsis stomatal development.","authors":"Mengyun Wu, Shiyuan Wang, Panpan Ma, Bixin Li, Huiqing Hu, Ziling Wang, Qin Qiu, Yujie Qiao, Dongdong Niu, Wolfgang Lukowitz, Shuqun Zhang, Mengmeng Zhang","doi":"10.1093/plcell/koae225","DOIUrl":"10.1093/plcell/koae225","url":null,"abstract":"An Arabidopsis (Arabidopsis thaliana) mitogen-activated protein kinase (MAPK) cascade composed of YODA (YDA)-MKK4/MKK5-MPK3/MPK6 plays an essential role downstream of the ERECTA (ER)/ER-LIKE (ERL) receptor complex in regulating stomatal development in the leaf epidermis. STOMAGEN (STO), a peptide ligand produced in mesophyll cells, competes with EPIDERMAL PATTERNING FACTOR2 (EPF2) for binding ER/ERL receptors to promote stomatal formation. In this study, we found that activation of MPK3/MPK6 suppresses STO expression. Using MUTE and STO promoters that confer epidermis- and mesophyll-specific expression, respectively, we generated lines with cell-specific activation and suppression of MPK3/MPK6. The activation or suppression of MPK3/MPK6 in either epidermis or mesophyll cells is sufficient to alter stomatal differentiation. Epistatic analyses demonstrated that STO overexpression can rescue the suppression of stomatal formation conferred by the mesophyll-specific expression of the constitutively active MKK4DD or MKK5DD, but not by the epidermis-specific expression of these constitutively active MKKs. These data suggest that STO is downstream of MPK3/MPK6 in mesophyll cells, but upstream of MPK3/MPK6 in epidermal cells in stomatal development signaling. This function of the MPK3/MPK6 cascade allows it to coordinate plant epidermis development based on its activity in mesophyll cells during leaf development.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonols improve tomato pollen thermotolerance during germination and tube elongation by maintaining reactive oxygen species homeostasis. 黄酮醇通过维持 ROS 的平衡来提高番茄花粉在萌芽和花管伸长过程中的耐热性。

IF 1 1区生物学

Plant Cell Pub Date : 2024-10-03 DOI: 10.1093/plcell/koae222

Anthony E Postiglione, Allison M Delange, Mohammad Foteh Ali, Eric Y Wang, Maarten Houben, Stacy L Hahn, Maleana G Khoury, Colleen M Roark, Molly Davis, Robert W Reid, James B Pease, Ann E Loraine, Gloria K Muday

{"title":"Flavonols improve tomato pollen thermotolerance during germination and tube elongation by maintaining reactive oxygen species homeostasis.","authors":"Anthony E Postiglione, Allison M Delange, Mohammad Foteh Ali, Eric Y Wang, Maarten Houben, Stacy L Hahn, Maleana G Khoury, Colleen M Roark, Molly Davis, Robert W Reid, James B Pease, Ann E Loraine, Gloria K Muday","doi":"10.1093/plcell/koae222","DOIUrl":"10.1093/plcell/koae222","url":null,"abstract":"Elevated temperatures impair pollen performance and reproductive success, resulting in lower crop yields. The tomato (Solanum lycopersicum) anthocyanin reduced (are) mutant harbors a mutation in FLAVANONE 3-HYDROXYLASE (F3H), resulting in impaired flavonol antioxidant biosynthesis. The are mutant has reduced pollen performance and seed set relative to the VF36 parental line, phenotypes that are accentuated at elevated temperatures. Transformation of are with the wild-type F3H gene, or chemical complementation with flavonols, prevented temperature-dependent reactive oxygen species (ROS) accumulation in pollen and restored the reduced viability, germination, and tube elongation of are to VF36 levels. Overexpression of F3H in VF36 prevented temperature-driven ROS increases and impaired pollen performance, revealing that flavonol biosynthesis promotes thermotolerance. Although stigmas of are had reduced flavonol and elevated ROS levels, the growth of are pollen tubes was similarly impaired in both are and VF36 pistils. RNA-seq was performed at optimal and stress temperatures in are, VF36, and the F3H overexpression line at multiple timepoints across pollen tube elongation. The number of differentially expressed genes increased over time under elevated temperatures in all genotypes, with the greatest number in are. These findings suggest potential agricultural interventions to combat the negative effects of heat-induced ROS in pollen that lead to reproductive failure.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-volume fully automated cell reconstruction generates a cell atlas of plant tissues. 大容量全自动细胞重建生成植物组织细胞图谱。

IF 1 1区生物学

Plant Cell Pub Date : 2024-09-16 DOI: 10.1093/plcell/koae250

Zijian Hu, Jiazheng Liu, Shiya Shen, Weiqian Wu, Jingbin Yuan, Weiwei Shen, Lingyu Ma, Guangchao Wang, Shunyao Yang, Xiuping Xu, Yaning Cui, Zhenchen Li, Lijun Shen, Linlin Li, Jiahui Bian, Xi Zhang, Hua Han, Jinxing Lin

{"title":"Large-volume fully automated cell reconstruction generates a cell atlas of plant tissues.","authors":"Zijian Hu, Jiazheng Liu, Shiya Shen, Weiqian Wu, Jingbin Yuan, Weiwei Shen, Lingyu Ma, Guangchao Wang, Shunyao Yang, Xiuping Xu, Yaning Cui, Zhenchen Li, Lijun Shen, Linlin Li, Jiahui Bian, Xi Zhang, Hua Han, Jinxing Lin","doi":"10.1093/plcell/koae250","DOIUrl":"https://doi.org/10.1093/plcell/koae250","url":null,"abstract":"The geometric shape and arrangement of individual cells play a role in shaping organ functions. However, analyzing multicellular features and exploring their connectomes in centimeter-scale plant organs remain challenging. Here, we established a set of frameworks named Large-Volume Fully Automated Cell Reconstruction (LVACR), enabling the exploration of three-dimensional (3D) cytological features and cellular connectivity in plant tissues. Through benchmark testing, our framework demonstrated superior efficiency in cell segmentation and aggregation, successfully addressing the inherent challenges posed by light sheet fluorescence microscopy (LSFM) imaging. Using LVACR, we successfully established a cell atlas of different plant tissues. Cellular morphology analysis revealed differences of cell clusters and shapes in between different poplar (P. simonii Carr. and P. canadensis Moench.) seeds, whereas topological analysis revealed that they maintained conserved cellular connectivity. Furthermore, LVACR spatiotemporally demonstrated an initial burst of cell proliferation, accompanied by morphological transformations at an early stage in developing the shoot apical meristem. During subsequent development, cell differentiation produced anisotropic features, thereby resulting in various cell shapes. Overall, our findings provided valuable insights into the precise spatial arrangement and cellular behavior of multicellular organisms, thus enhancing our understanding of the complex processes underlying plant growth and differentiation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":null,"pages":null},"PeriodicalIF":10.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0