Plant Cell最新文献_第7页

Mitochondrial glutamine synthetase overexpression in tomato source leaves increases assimilate export and fruit yield. 线粒体谷氨酰胺合成酶在番茄源叶片中的过表达增加了同化物的输出和果实产量。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag181

José G Vallarino, Laise Rosado-Souza, Youjun Zhang, R George Ratcliffe, Lee J Sweetlove, Sanu Shameer, Alisdair R Fernie

{"title":"Mitochondrial glutamine synthetase overexpression in tomato source leaves increases assimilate export and fruit yield.","authors":"José G Vallarino, Laise Rosado-Souza, Youjun Zhang, R George Ratcliffe, Lee J Sweetlove, Sanu Shameer, Alisdair R Fernie","doi":"10.1093/plcell/koag181","DOIUrl":"10.1093/plcell/koag181","url":null,"abstract":"To identify potential strategies for increasing the efficiency of tomato leaf metabolism with a focus on the links between nitrogen/carbon metabolism, we explored a diel flux balance analysis (FBA) model of a source leaf in which the metabolic output was varied up to the theoretically achievable maximum. We noticed a potentially interesting switch in the use of glutamine synthetase (GS) isoforms-from the chloroplast isoform to the mitochondrial one-for nitrogen assimilation. To further explore this prediction, we characterized transgenic tomato plants as overexpressing 2 tomato GS genes, GS1 and GS2, targeted to mitochondria. Both sets of transgenic plants were characterized as displaying faster growth rate, early flowering, and increased fruit yield. In leaves, metabolomic profiling and enzyme activity analysis pointed out that GS activity in mitochondria plays a role in increasing the intracellular synthesis and subsequent export of sugar. Consistent with these changes, higher sucrose concentration in leaf exudates and reduced activities of enzymes involved in leaf starch synthesis were observed. Moreover, mitochondrial GS activity affected chloroplast redox status in a manner that modulated photorespiration and nitrogen metabolism. The combined data reveal the influence of mitochondrial GS activity on both foliar carbon/nitrogen balance and regulation of source-sink metabolism in tomato plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding plants cell by cell: opportunities and challenges in single-cell and spatial biology. 植物细胞解码：单细胞与空间生物学的机遇与挑战。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag223

Lieven De Veylder, Maite Saura-Sanchez, Bert De Rybel, Tatsuya Nobori, Klaas Vandepoele, Lorenzo Caputi, Sarah E O'Connor, Jian Xu

{"title":"Decoding plants cell by cell: opportunities and challenges in single-cell and spatial biology.","authors":"Lieven De Veylder, Maite Saura-Sanchez, Bert De Rybel, Tatsuya Nobori, Klaas Vandepoele, Lorenzo Caputi, Sarah E O'Connor, Jian Xu","doi":"10.1093/plcell/koag223","DOIUrl":"10.1093/plcell/koag223","url":null,"abstract":"Recent advances in single-cell and spatial omics technologies are transforming plant research by enabling cell-resolved analyses of gene expression, proteomics, chromatin organization, and metabolism at the level of individual cells and at spatial resolution. These approaches have revealed extensive cellular heterogeneity, rare and transient cell states, and previously unrecognized developmental and physiological programs. However, they also introduce conceptual, technical, and computational challenges related to data quality and integration, and biological interpretation. In this review, we summarize the emerging opportunities and key bottlenecks in plant single-cell and spatial biology. We discuss how embedding single-cell data within evolutionary developmental frameworks can accelerate the discovery of functionally relevant genes, and how spatial transcriptomics may enable comparative study designs, the study of cell-to-cell communication, and multi-organism spatial analyses. We also explore how integrating multiple datatypes and gene regulatory network (GRN) inference can move the field from descriptive atlases toward mechanistic insights. Finally, we highlight the importance of visualization strategies across spatial, temporal, environmental, and evolutionary scales. Overall, we argue that careful experimental design, robust data integration, and a clear understanding of cell identity are essential to obtain the full potential of plant single-cell and spatial omics in basic research and crop improvement.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148621231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The NPR7-TGA6-MYB1 module promotes anthocyanin biosynthesis and integrates salicylic acid and strigolactone signaling in apple. 修正：NPR7-TGA6-MYB1模块促进苹果花青素的生物合成，整合水杨酸和独角甾内酯信号。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-08-04 DOI: 10.1093/plcell/koag243

引用次数: 0

Plant Reductases for Benzyl Alcohol Production in the Phenylalanine-Derived Salicylic Acid Biosynthesis Pathway. 苯丙氨酸衍生水杨酸生物合成途径中产生苯甲醇的植物还原酶。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-30 DOI: 10.1093/plcell/koag228

Lu Xu, Mingsong Wu, Dan Qiu, Josh Li, Chenying Li, Yanan Liu, Xin Li, Yuelin Zhang

引用次数: 0

A trio of Arabidopsis proteins acts as the executor complex in the pollen aperture formation program. 拟南芥蛋白在花粉孔形成程序中起着执行复合物的作用。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag163

Yuan Zhou, Crystal Finzer, Prativa Amom, Michelle Tan, Emili Toppari, Mia Scott, Jay Huang, Sarah H Reeder, Anna A Dobritsa

{"title":"A trio of Arabidopsis proteins acts as the executor complex in the pollen aperture formation program.","authors":"Yuan Zhou, Crystal Finzer, Prativa Amom, Michelle Tan, Emili Toppari, Mia Scott, Jay Huang, Sarah H Reeder, Anna A Dobritsa","doi":"10.1093/plcell/koag163","DOIUrl":"10.1093/plcell/koag163","url":null,"abstract":"Pollen apertures are specialized sites on the pollen surface that have little to no pollen wall exine and their numbers, positions, and morphology are species specific. Apertures have become a useful model for studying how cells develop distinct plasma membrane (PM) domains. During pollen development, these sites recruit specific factors and become protected from exine deposition. However, the molecular mechanisms underlying this process and the identities of the associated aperture factors remain poorly understood. Previous studies identified 2 Arabidopsis (Arabidopsis thaliana) proteins, INAPERTURATE POLLEN 1 (INP1) and the non-paralogous INP2, as essential for aperture formation and showed that they form a species-specific protein complex proposed to localize to the extracellular surface of the aperture PM domains. In this study, we identified STRUBBELIG RECEPTOR FAMILY 2 (SRF2), a receptor-like pseudokinase that spans the PM, as another factor required for pollen aperture formation in Arabidopsis. SRF2 shares key features with INP1 and INP2, including mutant phenotype, expression pattern, localization to aperture PM domains, genetic interactions, and evolutionary trends. We present evidence that the extracellular domain of SRF2 interacts with INP2, and that these 3 proteins form a complex essential for executing the aperture formation program, with properties that differ among species.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A GRAS transcription factor regulates far-red light responses via gibberellin-related compounds in Marchantia polymorpha. GRAS转录因子通过赤霉素相关化合物调节多形地药的远红光响应。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag188

Eita Shimokawa, Maiko Okabe, Rui Sun, Shogo Kawamura, Yoshihiro Yoshitake, Yukiko Yasui, Shohei Yamaoka, Kiyoshi Mashiguchi, Shinjiro Yamaguchi, Takayuki Kohchi

{"title":"A GRAS transcription factor regulates far-red light responses via gibberellin-related compounds in Marchantia polymorpha.","authors":"Eita Shimokawa, Maiko Okabe, Rui Sun, Shogo Kawamura, Yoshihiro Yoshitake, Yukiko Yasui, Shohei Yamaoka, Kiyoshi Mashiguchi, Shinjiro Yamaguchi, Takayuki Kohchi","doi":"10.1093/plcell/koag188","DOIUrl":"10.1093/plcell/koag188","url":null,"abstract":"Gibberellin (GA)-dependent regulation of growth and development is a critical innovation for environmental adaptation that arose during the evolution of land plants. While bioactive GA molecules known in angiosperms have not been detected in bryophytes, intermediates in GA biosynthesis, such as ent-kaurenoic acid (KA), are required for bryophyte development. However, the absence of orthologs of the known GA receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1) in bryophytes suggests the existence of an alternative pathway for the action of GA-related compounds. In the liverwort Marchantia polymorpha, far-red light (FR) responses are mediated by a yet-unknown GA-related compound (termed GAMp). Here we show that MpGRAS10, a GRAS family transcription factor, is likely involved in GAMp signaling. Functional analysis revealed that MpGRAS10 is required for FR responses, including hyponastic thallus growth and gametangiophore formation. The insensitivity of Mpgras10 mutants to KA suggests that MpGRAS10 acts downstream of KA biosynthesis. Moreover, MpGRAS10 gain of function caused morphological and transcriptomic changes similar to FR responses under white-light conditions, as well as increased sensitivity to KA treatment. Phylogenetic analysis demonstrated that MpGRAS10 belongs to a GRAS clade containing orthologs in bryophytes, lycophytes and ferns, but not seed plants. These findings provide insights into the diversity and evolution of GA-related signaling pathways in land plants.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HHO5 orchestrates dose-dependent feedback regulation of organic versus inorganic nitrogen signaling in Arabidopsis. HHO5协调拟南芥有机与无机氮信号的剂量依赖反馈调节。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag201

Will E Hinckley, Joseph Swift, Francisco Romei, Jorge P Muschietti, Samantha Frangos, Shao-Shan Carol Huang, Gloria M Coruzzi, Mariana Obertello

{"title":"HHO5 orchestrates dose-dependent feedback regulation of organic versus inorganic nitrogen signaling in Arabidopsis.","authors":"Will E Hinckley, Joseph Swift, Francisco Romei, Jorge P Muschietti, Samantha Frangos, Shao-Shan Carol Huang, Gloria M Coruzzi, Mariana Obertello","doi":"10.1093/plcell/koag201","DOIUrl":"10.1093/plcell/koag201","url":null,"abstract":"A major goal in agriculture is to engineer crops to maintain yield with less nitrogen (N) fertilizer. Important regulators of plant N-responses include HRS1 HOMOLOG (HHO) transcription factors (TFs); yet, their redundant repressive mode of action hinders functional analyses. Here, we highlight HHO5 as a unique HHO TF based on its phylogenetic position, phloem specific expression, and dual role in regulating responses to inorganic and organic N-dose signals. Our results support a model whereby HHO5 mediates feedback repression of inorganic nitrate uptake under organic N satiety: (i) Meta-analyses revealed HHO5 expression is repressed by inorganic N, but induced by organic N treatments. (ii) HHO5 directly binds and represses nitrate response genes, but indirectly induces organic N-response genes. (iii) HHO5 indirect target gene induction occurs via WRKY partner TFs, validated using a cell-based DoubleTARGET TF co-perturbation assay. (iv) HHO5 initiates a validated gene regulatory network path which encompasses ∼12% of the N-dose response genes in planta. (v) Phenotypically, single hho5 T-DNA mutants show reduced N-dose dependent growth, and decreased seed N content. (vi) HHO5 represses nitrate uptake but induces root growth responses to Glu in planta. Overall, our findings support a model wherein HHO5 orchestrates dose dependent feedback regulation of organic versus inorganic N-signaling in Arabidopsis.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":"38 7","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13421923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148631279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quenching the thirst internally: separating miRNA from target mRNA by condensation of AGO1. 内部解渴：通过AGO1的缩合将miRNA与靶mRNA分离。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag175

Pei Qin Ng

引用次数: 0

The hidden life of unicellular red algae: discovery of a common sexual cycle unlocks a model lineage for eukaryotic evolution. 单细胞红藻的隐藏生命：发现一个共同的性循环，为真核生物进化打开了一个模式谱系。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag178

Matteo Pivato

引用次数: 0

Sexual life cycle establishes the unicellular red algae Cyanidiophyceae as a genetically tractable model for eukaryotic evolution. 有性生命周期建立了单细胞红藻蓝藻作为真核生物进化的遗传可处理模型。

IF 13.5 1区生物学

Plant Cell Pub Date : 2026-07-02 DOI: 10.1093/plcell/koag148

Shunsuke Hirooka, Takayuki Fujiwara, Mark Seger, Soichi Inagaki, Shota Yamashita, Dai Tsujino, Ryo Onuma, Yu Kanesaki, Satoru Watanabe, Yuu Hirose, Ryudo Ohbayashi, Mari Takusagawa, Baifeng Zhou, Reiko Tomita, Fumi Yagisawa, Peter Lammers, Atsuko H Iwane, Shin-Ya Miyagishima

{"title":"Sexual life cycle establishes the unicellular red algae Cyanidiophyceae as a genetically tractable model for eukaryotic evolution.","authors":"Shunsuke Hirooka, Takayuki Fujiwara, Mark Seger, Soichi Inagaki, Shota Yamashita, Dai Tsujino, Ryo Onuma, Yu Kanesaki, Satoru Watanabe, Yuu Hirose, Ryudo Ohbayashi, Mari Takusagawa, Baifeng Zhou, Reiko Tomita, Fumi Yagisawa, Peter Lammers, Atsuko H Iwane, Shin-Ya Miyagishima","doi":"10.1093/plcell/koag148","DOIUrl":"10.1093/plcell/koag148","url":null,"abstract":"The thermo-acidophilic unicellular algal class Cyanidiophyceae diverged from other red algae soon after primary chloroplast acquisition. Cyanidiophyceae possess extremely simple genomes (8.7 to 17.8 Mb; approximately 4,800 to 7,800 genes), and the cell wall-less, genetically tractable strain Cyanidioschyzon merolae 10D has served as a model organism. However, its unknown sexual life cycle has limited its utility in studies of evolution and genetics. Here, we show that in the genera Cyanidioschyzon, Cyanidiococcus, and Cyanidium, the cell-walled diploid form, exclusively observed in nature, produces a cell wall-less haploid form when the culture pH is lowered, and both proliferate asexually. Cz. merolae 10D is a haploid clone that forms a cell-walled diploid through mating with other haploid clones. In addition, we generated high-quality genomic resources with phase-specific transcriptomes, identified a compact candidate mating-type region, and developed genetic manipulation systems using the cell wall-less haploids of these genera. We further uncovered phase-specific distributions of histone H3 lysine 27 trimethylation linked to haploid- and diploid-specific gene expression, including transcription factors involved in differentiation associated with sexual reproduction in plants. Additionally, biparental inheritance of organelle DNA occurs following isogamous mating of haploid cells but resolves into uniparental inheritance during diploid proliferation. These advances position Cyanidiophyceae as a powerful model lineage for studying early Archaeplastida evolution, the shared mechanisms of photosynthetic eukaryotes, and environmental adaptation.","PeriodicalId":20186,"journal":{"name":"Plant Cell","volume":" ","pages":""},"PeriodicalIF":13.5,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13353863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0