Planta最新文献

{"title":"Cytoplasmic male sterility-based hybrids: mechanistic insights.","authors":"Joorie Bhattacharya, Rahul B Nitnavare, Pooja Bhatnagar-Mathur, Palakolanu Sudhakar Reddy","doi":"10.1007/s00425-024-04532-w","DOIUrl":"10.1007/s00425-024-04532-w","url":null,"abstract":"<p><strong>Main conclusion: </strong>A comprehensive understanding of the nucleocytoplasmic interactions that occur between genes related to the restoration of fertility and cytoplasmic male sterility (CMS) provides insight into the development of hybrids of important crop species. Modern biotechnological techniques allow this to be achieved in an efficient and quick manner. Heterosis is paramount for increasing the yield and quality of a crop. The development of hybrids for achieving heterosis has been well-studied and proven to be robust and efficient. Cytoplasmic male sterility (CMS) has been explored extensively in the production of hybrids. The underlying mechanisms of CMS include the role of cytotoxic proteins, PCD of tapetal cells, and improper RNA editing of restoration factors. On the other hand, the restoration of fertility is caused by the presence of restorer-of-fertility (Rf) genes or restorer genes, which inhibit the effects of sterility-causing genes. The interaction between mitochondria and the nuclear genome is crucial for several regulatory pathways, as observed in the CMS-Rf system and occurs at the genomic, transcriptional, post-transcriptional, translational, and post-translational levels. These CMS-Rf mechanisms have been validated in several crop systems. This review aims to summarize the nucleo-mitochondrial interaction mechanism of the CMS-Rf system. It also sheds light on biotechnological interventions, such as genetic engineering and genome editing, to achieve CMS-based hybrids.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 4","pages":"100"},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CsPPR gene with RNA-editing function involved in leaf color asymmetry of the reciprocal hybrids derived from Cucumis sativus and C. hystrix.","authors":"Lei Xia, Han Wang, Xiaokun Zhao, Qinzheng Zhao, Xiaqing Yu, Ji Li, Qunfeng Lou, Jinfeng Chen, Chunyan Cheng","doi":"10.1007/s00425-024-04513-z","DOIUrl":"10.1007/s00425-024-04513-z","url":null,"abstract":"<p><strong>Main conclusion: </strong>The leaf color asymmetry found in the reciprocal hybrids C. hystrix × C. sativus (HC) and C. sativus × C. hystrix (CH) could be influenced by the CsPPR gene (CsaV3_1G038250.1). Most angiosperm organelles are maternally inherited; thus, the reciprocal hybrids usually exhibit asymmetric phenotypes that are associated with the maternal parent. However, there are two sets of organelle genomes in the plant cytoplasm, and the mechanism of reciprocal differences are more complex and largely unknown, because the chloroplast genes are involved besides mitochondrial genes. Cucumis spp. contains the species, i.e., cucumber and melon, which chloroplasts and mitochondria are maternally inherited and paternally inherited, respectively, serving as good materials for the study of reciprocal differences. In this study, leaf color asymmetry was observed in the reciprocal hybrids (HC and CH) derived from C. sativus (2n = 14, CC) and C. hystrix (2n = 24, HH), where the leaves of HC were found to have reduced chlorophyll content, abnormal chloroplast structure and lower photosynthetic capacity. Transcriptomic analysis revealed that the chloroplast development-related genes were differentially expressed in leaf color asymmetry. Genetic analysis showed that leaf color asymmetry was caused by the maternal chloroplast genome. Comparative analysis of chloroplast genomes revealed that there was no mutation in the chloroplast genome during interspecific hybridization. Moreover, a PPR gene (CsaV3_1G038250.1) with RNA-editing function was found to be involved in the regulation of leaf color asymmetry. These findings provide new insights into the regulatory mechanisms of asymmetric phenotypes in plant reciprocal crosses.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 4","pages":"102"},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological approaches to reduce the phytic acid content in millets to improve nutritional quality","authors":"Bhuvnesh Sareen, Ramesh Namdeo Pudake, Amitha Mithra Sevanthi, Amolkumar U. Solanke","doi":"10.1007/s00425-024-04525-9","DOIUrl":"https://doi.org/10.1007/s00425-024-04525-9","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>The review article summarizes the approaches and potential targets to address the challenges of anti-nutrient like phytic acid in millet grains for nutritional improvement.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Millets are a diverse group of minor cereal grains that are agriculturally important, nutritionally rich, and the oldest cereals in the human diet. The grains are important for protein, vitamins, macro and micronutrients, fibre, and energy sources. Despite a high amount of nutrients, millet grains also contain anti-nutrients that limit the proper utilization of nutrients and finally affect their dietary quality. Our study aims to outline the genomic information to identify the target areas of research for the exploration of candidate genes for nutritional importance and show the possibilities to address the presence of anti-nutrient (phytic acid) in millets. So, the physicochemical accessibility of micronutrients increases and the agronomic traits can do better. Several strategies have been adopted to minimize the phytic acid, a predominant anti-nutrient in cereal grains. In the present review, we highlight the potential of biotechnological tools and genome editing approaches to address phytic acid in millets. It also highlights the biosynthetic pathway of phytic acid and potential targets for knockout or silencing to achieve low phytic acid content in millets.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"3 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the evolutionary development of the “Chinese lantern” within Solanaceae","authors":"Lanfeng Wu, Qianqian Liu, Wei Gou, Jun Li, Qianhui Cao, Chaoying He","doi":"10.1007/s00425-024-04535-7","DOIUrl":"https://doi.org/10.1007/s00425-024-04535-7","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>The key genetic variation underlying the evo-devo of ICS in Solanaceae may be further pinpointed using an integrated strategy of forward and reverse genetics studies under the framework of phylogeny.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The calyx of <i>Physalis</i> remains persistent throughout fruit development. Post-flowering, the fruiting calyx is inflated rapidly to encapsulate the berry, giving rise to a “Chinese lantern” structure called inflated calyx syndrome (ICS). It is unclear how this novelty arises. Over the past 2 decades, the role of MADS-box genes in the evolutionary development (evo-devo) of ICS has mainly been investigated within Solanaceae. In this review, we analyze the main achievements, challenges, and new progress. ICS acts as a source for fruit development, provides a microenvironment to protect fruit development, and assists in long-distance fruit dispersal. ICS is a typical post-floral trait, and the onset of its development is triggered by specific developmental signals that coincide with fertilization. These signals can be replaced by exogenous gibberellin and cytokinin application. <i>MPF2-like</i> heterotopic expression and <i>MBP21-like</i> loss have been proposed to be two essential evolutionary events for ICS origin, and manipulating the related MADS-box genes has been shown to affect the ICS size, sepal organ identity, and/or male fertility, but not completely disrupt ICS. Therefore, the core genes or key links in the ICS biosynthesis pathways may have undergone secondary mutations during evolution, or they have not yet been pinpointed. Recently, we have made some encouraging progress in acquiring lantern mutants in <i>Physalis floridana</i>. In addition to technological innovation, we propose an integrated strategy to further analyze the evo-devo mechanisms of ICS in Solanaceae using forward and reverse genetics studies under the framework of phylogeny.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"119 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dominant white color trait of the melon fruit rind is associated with epicuticular wax accumulation","authors":"Ran Ezer, Ekaterina Manasherova, Amit Gur, Arthur A. Schaffer, Yaakov Tadmor, Hagai Cohen","doi":"10.1007/s00425-024-04527-7","DOIUrl":"https://doi.org/10.1007/s00425-024-04527-7","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main Conclusion</h3><p>Microscopic analyses and chemical profiling demonstrate that the white rind phenotype in melon fruit is associated with the accumulation of n-alkanes, fatty alcohols, aldehydes and wax esters.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Serving as an indicator of quality, the rind (or external) color of fruit directly affects consumer choice. A fruit’s color is influenced by factors such as the levels of pigments and deposited epicuticular waxes. The latter produces a white-grayish coating often referred to as “wax bloom”. Previous reports have suggested that some melon (<i>Cucumis melo</i> L.) accessions may produce wax blooms, where a dominant white rind color trait was genetically mapped to a major locus on chromosome 7 and suggested to be inherited as a single gene named <i>Wi</i>. We here provide the first direct evidence of the contribution of epicuticular waxes to the dominant white rind trait in melon fruit. Our light and electron microscopy and gas chromatography-mass spectrometry (GC–MS) comparative analysis of melon accessions with white or green rinds reveals that the rind of melon fruit is rich in epicuticular waxes. These waxes are composed of various biochemical classes, including fatty acids, fatty alcohols, aldehydes, fatty amides, <i>n</i>-alkanes, tocopherols, triterpenoids, and wax esters. We show that the dominant white rind phenotype in melon fruit is associated with increased accumulation of <i>n</i>-alkanes, fatty alcohols, aldehydes and wax esters, which are linked with the deposition of crystal-like wax platelets on their surfaces. Together, this study broadens the understanding of natural variation in an important quality trait of melon fruit and promotes the future identification of the causative gene for the dominant white rind trait.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":20177,"journal":{"name":"Planta","volume":"197 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142265914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of calcium ions and cell wall deposition on the pollen viability of Paeonia lactiflora after cryopreservation","authors":"Shangqian Liu, Mengting Zhu, Wenjie Ma, Yingling Wan, Yan Liu","doi":"10.1007/s00425-024-04530-y","DOIUrl":"https://doi.org/10.1007/s00425-024-04530-y","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>Four cultivars of <i>Paeonia lactiflora</i> pollen have a different viability after cryopreservation, and that the difference of pollen viability is related to calcium ions and cell wall deposition.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Cryopreservation is a vital technique for preserving germplasm resources, offering extensive application prospects. Understanding the factors influencing pollen viability after cryopreservation is crucial for the permanent preservation and exchange of pollen resources. This study investigated pollen from four <i>Paeonia lactiflora</i> cultivars with varying viability after cryopreservation, aiming to determine whether calcium ions (Ca²⁺) and cell wall deposition affect these viability changes. The results showed that Ca²⁺-ATPase activity and cytoplasmic Ca²⁺ of all four cultivars exhibited an increasing trend after cryopreservation; the calmodulin (CaM) content varied with cultivars. Correlation analysis showed that fresh pollen viability was significantly negatively correlated with cytoplasmic Ca²⁺ content and positively correlated with Ca²⁺-ATPase activity, while pollen viability after cryopreservation exhibited a significantly negative correlation with cytoplasmic Ca²⁺ content and a positive correlation with CaM content. The pollen cell wall of the cultivar ‘Zi Feng Chao Yang’ (ZFCY), which showed increased viability after cryopreservation, contained significantly higher levels of low-temperature tolerance-related phospholipids and proteins compared to other cultivars. Additionally, all cultivars maintained a clear Ca²⁺ gradient at the tips of pollen tubes after cryopreservation, without significant callose accumulation. These findings suggest that differences in Ca²⁺ signaling and cell wall components deposition influence changes in pollen viability after cryopreservation, and the Ca²⁺ gradient and callose at the tip of pollen tubes are not responsible for preventing pollen tube growth.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"21 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-type specific localization and biological activity of the volatiles from the endemic species Chaerophyllum coloratum L.","authors":"Elma Vuko, Sanja Radman, Ivana Bočina, Juraj Kamenjarin, Ivana Bezmalinović, Željana Fredotović","doi":"10.1007/s00425-024-04531-x","DOIUrl":"https://doi.org/10.1007/s00425-024-04531-x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>New findings are presented for <i>Chaerophyllum coloratum</i> L. on the volatile composition of the essential oil, based on data of hydrosol and fresh plant material, light and electron microscopy of leaves, and cytotoxic and antiviral activity.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The widespread Apiaceae family includes many well-known and economically important plants that are cultivated as food or spices. Many produce essential oils and are generally a source of secondary metabolites and compounds that have numerous applications in daily life. In this study, the chemical composition of volatile organic compounds (VOCs), ultrastructure and biological activity of the Mediterranean endemic species <i>Cheaerophyllum coloratum</i> L. are investigated, as literature data for this plant species are generally very scarce. The essential oil and hydrosol were extracted from the air-dried leaves by hydrodistillation and the chemical composition of both extracts was analysed by GC–MS in conjunction with headspace solid-phase microextraction (HS-SPME) of VOCs from the hydrosol and the fresh plant material. In the composition of the essential oil, the oxygenated sesquiterpenes spathulenol and caryophyllene oxide were the most abundant components. In the fresh plant material, non-oxygenated sesquiterpenes dominated, with β-caryophyllene and germacrene D being the main components. The hydrosol was dominated by monoterpenes, with the oxygenated monoterpene <i>p</i>-cymen-8-ol being the most abundant. Light and electron micrographs of the leaf of <i>C. coloratum</i> show secretory structures, and we hypothesize that glandular leaf trichomes, secretory epidermal cells and secretory canals are involved in the production of volatiles and their secretion on the leaf surface. Since the biological potential of <i>C. coloratum</i> is poorly investigated, we tested its cytotoxic activity on cancer and healthy cell lines and its antiviral activity on plants infected with tobacco mosiac virus (TMV). Our results dealing with the composition, ultrastructure and biological activity show that <i>C. coloratum</i> represent a hidden valuable plant species with a potential for future research.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"41 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142269831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-control of soil-borne virus infection by seed application of Glycyrrhiza glabra extract and the rhamnolipid Rhapynal","authors":"Viktoria Fomitcheva, Claudia J. Strauch, Sabine Bonse, Petra Bauer, Thomas Kühne, Annette Niehl","doi":"10.1007/s00425-024-04529-5","DOIUrl":"https://doi.org/10.1007/s00425-024-04529-5","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>Seed-application of the natural products protects sugar beet and wheat plants against infection with plasmodiophorid-transmitted viruses and thus may represent an efficient, environmentally friendly, easy and cost effective biocontrol strategy.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In times of intensive agriculture, resource shortening and climate change, alternative, more sustainable and eco-friendly plant protection strategies are required. Here, we tested the potential of the natural plant substances <i>Glycyrrhiza glabra</i> leaf extract (GE) and the rhamnolipid Rhapynal (Rha) applied to seeds to protect against infection of sugar beet and wheat with soil-borne plant viruses. The soil-borne <i>Polymyxa betae-</i> and <i>Polymyxa graminis</i>-transmitted viruses cause extensive crop losses in agriculture and efficient control strategies are missing. We show that GE and Rha both efficiently protect plants against infection with soil-borne viruses in sugar beet and wheat when applied to seeds. Moreover, the antiviral protection effect is independent of the cultivar used. No protection against <i>Polymyxa sp.</i> was observed after seed treatment with the bio-substances at our analysis time points<i>.</i> However, when we applied the bio-substances directly to soil a significant anti-<i>Polymyxa graminis</i> effect was obtained in roots of barley plants grown in the soil as well as in the treated soil. Despite germination can be affected by high concentrations of the substances, a range of antiviral protection conditions with no effect on germination were identified. Seed-treatment with the bio-substances did not negatively affect plant growth and development in virus-containing soil, but was rather beneficial for plant growth. We conclude that seed treatment with GE and Rha may represent an efficient, ecologically friendly, non-toxic, easy to apply and cost efficient biocontrol measure against soil-borne virus infection in plants.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"12 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142184267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in understanding the roles of plant HAT and HDAC in non-histone protein acetylation and deacetylation","authors":"Zihan Zhang, Yan Zeng, Jiaqi Hou, Lijia Li","doi":"10.1007/s00425-024-04518-8","DOIUrl":"https://doi.org/10.1007/s00425-024-04518-8","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>This review focuses on HATs and HDACs that modify non-histone proteins, summarizes functional mechanisms of non-histone acetylation as well as the roles of HATs and HDACs in rice and Arabidopsis.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The growth and development of plants, as well as their responses to biotic and abiotic stresses, are governed by intricate gene and protein regulatory networks, in which epigenetic modifying enzymes play a crucial role. Histone lysine acetylation levels, modulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs), are well-studied in the realm of transcriptional regulation. However, the advent of advanced proteomics has unveiled that non-histone proteins also undergo acetylation, with its underlying mechanisms now being clarified. Indeed, non-histone acetylation influences protein functionality through diverse pathways, such as modulating protein stability, adjusting enzymatic activity, steering subcellular localization, influencing interactions with other post-translational modifications, and managing protein–protein and protein–DNA interactions. This review delves into the recent insights into the functional mechanisms of non-histone acetylation in plants. We also provide a summary of the roles of HATs and HDACs in rice and Arabidopsis, and explore their potential involvement in the regulation of non-histone proteins.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"157 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142184269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential salt stress resistance in male and female Salix linearistipularis plants: insights from transcriptome profiling and the identification of the 4-hydroxy-tetrahydrodipicolinate synthase gene","authors":"Delong Fan, Weichao Fu, Lixin Li, Shenkui Liu, Yuanyuan Bu","doi":"10.1007/s00425-024-04528-6","DOIUrl":"https://doi.org/10.1007/s00425-024-04528-6","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Main conclusion</h3><p>Lysine plays an essential role in the growth differences between male and female <i>S. linearistipularis</i> plants under salt stress. Furthermore, <i>SlDHDPS</i> is identified as a vital gene contributing to the differences in saline-alkali tolerance between male and female plants of <i>S. linearistipularis</i>.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Soil salinization is a significant problem that severely restricts agricultural production worldwide. High salinity and low nutrient concentrations consequently prevent the growth of most plant species. <i>Salix linearistipularis</i> is the only woody plant (shrub) naturally distributed in the saline-alkali lands of the Songnen Plain in Northeast China, and it is one of the few plants capable of thriving in soils with extremely high salt and alkaline pH (&gt;9.0) levels. However, insufficient attention has been given to the interplay between salt and nitrogen in the growth and development of <i>S. linearistipularis</i>. Here, the male and female plants of <i>S. linearistipularis</i> were subjected to salt stress with nitrogen-starvation or nitrogen-supplement treatments, and it was found that nitrogen significantly affects the difference in salt tolerance between male and female plants, with nitrogen-starvation significantly enhancing the salt stress tolerance of female plants compared to male plants. Transcriptional analyses showed 66 differentially expressed nitrogen-responsive genes in female and male roots, with most of them showing sexual differences in expression patterns under salinity stress. RNA-seq and RT-qPCR analysis demonstrated that six genes had an opposite salt-induced expression pattern in female and male roots. The expression of the 4-hydroxy-tetrahydrodipicolinate synthase encoding gene (<i>SlDHDPS</i>) in female roots was higher than that in male roots. Further treatment with exogenous lysine could significantly alleviate the inhibitory effect of salt stress on the growth of female and male plants. These results indicate that the <i>SlDHDPS</i> in the nitrogen metabolism pathway is involved in the resistance of <i>S. linearistipularis</i> to salt stress, which lays a foundation for further exploring the mechanism of nitrogen on salt tolerance of <i>S. linearistipularis</i>, and has a significant reference value for saline-alkali land management and sustainable agricultural development.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"24 1","pages":""},"PeriodicalIF":4.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142184270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}