内含子分裂microRNA介导茄科植物低磷根编码mRNA的裂解。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-01-07 DOI:10.1007/s00425-024-04596-8
Zahara Medina-Calzada, Runchun Jing, Simon Moxon, Hong Zhu, Ping Xu, Tamas Dalmay
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引用次数: 0

摘要

主要结论:一个具有非规范前体结构的microRNA在其miRNA-5p和miRNA-3p之间含有一个内含子,与其生物发生相关,在茄科植物中是保守的,并且靶向低磷根的mRNA。在植物中已鉴定出数百种miRNA,对植物miRNA的生物发生、机制和功能的认识取得了很大进展。尽管如此,许多mirna,特别是那些不太传统的特征,仍有待发现。同样,从miRNA产生到作用和转换的其他调控层仍在揭示中。目前的研究描述了一种以前未被发现的microRNA,因为它具有不寻常的内含子分裂茎环结构,以前只在单株特异性miRNA444家族中观察到。它显示了它在茄属植物的一个分支中的保守性,包括与农业相关的茄科,其中它的转录本已经在序列数据库中的几个物种中被预测。该miRNA在拟南芥中不存在,但在番茄茄、本烟、矮牵牛花和薄荷中存在。这证明了该miRNA基因至少产生了两种不同的pri-miRNA变体,一种是剪接的,另一种保留内含子。它证明了内含子在miRNA生物发生中的双重功能。一方面,它在pri-miRNA中的存在对成熟miRNA的积累有积极的影响,但另一方面,它需要从pri-miRNA中去除,才能有效地产生成熟miRNA。最后,它将低磷根作为其靶标之一,这是一种已知参与其他植物在磷酸盐饥饿下根系生长调节的蛋白质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An intron-split microRNA mediates cleavage of the mRNA encoded by low phosphate root in Solanaceae.

Main conclusion: A microRNA with a non-canonical precursor structure harbours an intron in between its miRNA-5p and miRNA-3p relevant for its biogenesis, is conserved across Solanaceae, and targets the mRNA of low phosphate root. Hundreds of miRNAs have been identified in plants and great advances have been accomplished in the understanding of plant miRNA biogenesis, mechanisms and functions. Still, many miRNAs, particularly those with less conventional features, remain to be discovered. Likewise, additional layers of regulation from miRNA generation to action and turnover are still being revealed. The current study describes a microRNA not previously identified given its unusual intron-split stem-loop structure, that has been previously observed only within the monocot-specific miRNA444 family. It shows its conservation across a branch of Solanales including agriculturally relevant Solanaceae family, where its transcripts had already been predicted in several species within sequence databases. The miRNA is absent in Arabidopsis thaliana but present in Solanum lycopersicum, Nicotiana benthamiana, Petunia axillaris, and Ipomoea nil. It proves that at least two different pri-miRNA variants are produced from this miRNA gene, one spliced and the other one retaining the intron. It demonstrates the dual function of its intron in the miRNA biogenesis. On the one hand, its presence in the pri-miRNA positively influences mature miRNA accumulation, but on the other hand, it needs to be removed from the pri-miRNA for efficient mature miRNA production. Finally, it sets low phosphate root as one of its targets, a protein known to be involved in root growth regulation under phosphate starvation in other plant species.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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