Exploring the plant-growth promoting bacterium Herbaspirillum seropedicae as catalyst of microbiome remodeling and metabolic changes in wheat plants.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-01-14 DOI:10.1007/s00425-025-04609-0
Pablo Carril, Carlos Cordeiro, Marta Sousa Silva, Ephrem Ngendahimana, Rogério Tenreiro, Cristina Cruz
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Abstract

Main conclusion: Inoculation with the PGPB Herbaspirillum seropedicae shapes both the structure and putative functions of the wheat microbiome and causes changes in the levels of various plant metabolites described to be involved in plant growth and health. Plant growth promoting bacteria (PGPB) can establish metabolic imprints in their hosts, contributing to the improvement of plant health in different ways. However, while PGPB imprints on plant metabolism have been extensively characterized, much less is known regarding those affecting plant indigenous microbiomes, and hence it remains unknown whether both processes occur simultaneously. In this study, both 16S amplicon and ITS sequencing analyses were carried out to study both the structural as well as the putative functional changes in the seed-borne endophytic microbiome of wheat plants inoculated with the PGPB Herbaspirillum seropedicae strain RAM10. Concomitantly, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) analyses were used to investigate the alterations in the root metabolome of PGPB-inoculated plants. PGPB inoculation led to marked differences in the composition of the root microbiome, accompanied by the differential enrichment of microorganisms with putative roles in both plant energy and nitrogen metabolism. In addition, metabolome analyses showed that the levels of 16 metabolites belonging to the phenylpropanoid, terpenoid, and unsaturated fatty acid families were significantly altered in PGPB-inoculated plants. These findings shed light on the interplay between PGPB, the plant and its associated microbiome, indicating that PGPB can act as the driving force mediating long-lasting changes in both the plant metabolome and the plant microbiome.

探索促进植物生长的 Herbaspirillum seropedicae 细菌作为小麦植物微生物组重塑和代谢变化的催化剂。
主要结论:接种ppgpb可改变小麦微生物群的结构和功能,并引起与植物生长和健康有关的各种植物代谢物水平的变化。植物生长促进菌(Plant growth promoting bacteria, PGPB)可以在寄主体内建立代谢印记,以不同的方式促进植物健康。然而,虽然ppgpb对植物代谢的印迹已经被广泛表征,但对影响植物本地微生物组的印迹知之甚少,因此仍然不清楚这两个过程是否同时发生。本研究通过16S扩增子分析和ITS测序分析,研究了接种PGPB seropedicae Herbaspirillum RAM10后小麦种传内生微生物群的结构变化和功能变化。同时,利用傅里叶变换离子回旋共振质谱(FT-ICR-MS)分析研究了pgpb接种植株根系代谢组的变化。接种PGPB导致根系微生物组组成的显著差异,并伴有可能在植物能量和氮代谢中发挥作用的微生物的富集差异。此外,代谢组学分析表明,pgpb接种植株的16种代谢产物(苯丙酸、萜类和不饱和脂肪酸家族)的水平发生了显著变化。这些发现揭示了PGPB与植物及其相关微生物组之间的相互作用,表明PGPB可以作为介导植物代谢组和植物微生物组长期变化的驱动力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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