Planta最新文献

{"title":"Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora.","authors":"Takuya Teshima, Keiichirou Nemoto, Motoki Shimizu, Chiharu Yoshida, Akiko Hirabuchi, Fumina Goto, Takashi Nakasato, Zenbi Naito, Masahiro Nishihara","doi":"10.1007/s00425-025-04772-4","DOIUrl":"10.1007/s00425-025-04772-4","url":null,"abstract":"<p><strong>Main conclusion: </strong>This study identified GeBCAT2 as a key gene in catalyzing the first step of branched chain amino acid biosynthesis in Gentiana triflora, thereby contributing to unpleasant floral odor emission. Gentians, widely cultivated as ornamental flowers in Japan, primarily originate from the endemic gentian species Gentiana triflora and G. scabra. This study analyzed volatile compounds in Japanese gentians using gas chromatography-mass spectrometry. Results showed that G. triflora flowers consistently emitted 3-methylbutanoic acid, 2-methylbutanoic acid, and isobutyric acid, which are volatile organic compounds derived from branched-chain amino acids (BCAAs) and associated with unpleasant odors. In contrast, G. scabra flowers did not emit these compounds. Although the BCAA metabolism has been widely studied, its catabolic pathways in gentians remain unclear. Therefore, we performed precursor feeding experiments to quantitatively verify the role of BCAAs and their corresponding keto acids in producing odorous volatiles. We also cloned and functionally analyzed two Gentiana BCAAs transferase genes (GeBCAT1 and GeBCAT2). Both genes were more highly expressed in flowers than in leaves, with expression levels higher in G. triflora than in G. scabra. Enzymatic assays with recombinant proteins demonstrated that GeBCAT1 and GeBCAT2 participate in BCAA-related catabolic reactions. Notably, GeBCAT2's substrate specificity for BCAAs correlated with unpleasant odor intensity in G. triflora, suggesting that it serves as the primary enzyme initiating unpleasant odor biosynthesis in gentians. These findings provide valuable insights into volatile biosynthesis in gentians and offer a foundation for breeding cultivars with reduced unpleasant odors.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"62"},"PeriodicalIF":3.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitinase-activity-independent anti-insect effect of class V chitinase from fig trees (Ficus carica).","authors":"Sakihito Kitajima, Toshiharu Akino, Hideki Yoshida, Kenji Miura, Toki Taira, Kazufumi Yazaki, Ryosuke Munakata, Yutori Murata, Miku Sugimori, Naoki Tani, Eric Hyrmeya Savadogo","doi":"10.1007/s00425-025-04782-2","DOIUrl":"10.1007/s00425-025-04782-2","url":null,"abstract":"<p><strong>Main conclusion: </strong>The feeding assay revealed growth-retardation effects of class V chitinase of Ficus carica on Spodoptera litura larvae and these effects were retained in its chitinase-inactive mutants. This study investigated the anti-insect activity of the class V chitinase (FcChiC) from latex of the fig tree (Ficus carica, Moraceae). The feeding of radish cotyledons, transiently producing FcChiC, to larvae of Spodoptera litura (Lepidoptera), resulted in significant growth reduction. A close homolog from Cannabis sativa (Cannabaceae) also demonstrated similar effects, while six other class V chitinases from plants, including Arabidopsis thaliana ChiC, did not. Notably, the anti-insect activity of FcChiC persisted in its chitinase-inactive mutants, which maintained chitin-binding ability. These results suggested that FcChiC and its C. sativa homolog are promising candidates for further research in pest control strategies.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"61"},"PeriodicalIF":3.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of cytokinin receptors in Arabidopsis thaliana seed development and how they affect the metabolomic profile.","authors":"Domenico Loperfido, Cecilia Zumajo-Cardona, Mauro Commisso, Flavia Guzzo, Barbara Ambrose, Lucia Colombo, Ignacio Ezquer","doi":"10.1007/s00425-025-04745-7","DOIUrl":"10.1007/s00425-025-04745-7","url":null,"abstract":"<p><strong>Main conclusion: </strong>Based on expression, functional, and metabolomic analyses in the seeds of the single-receptor mutants, each receptor has a specific function during seed development. Their redundant roles during this process are difficult to assess; moreover, the impact they have on plant development must also be taken into account. In this study, we investigated the role of cytokinin receptors in Arabidopsis thaliana seed development and their impact on the metabolomic profile. Our findings reveal distinct expression patterns among them in the seed: AHK2 expression is not detected in seed tissues, AHK3 is expressed in embryo, endosperm, and peripheral endosperm, while AHK4/CRE1 expression is restricted to a few embryo cells. These patterns are consistent with the observed phenotypes where ahk3 exhibits more severe seed phenotypes such as delayed embryo development and increased seed and endosperm size. Metabolomic analyses showed that the receptors impact the abundance of metabolites, with a remarkably high concentration of tannins in ahk2 with respect to wild type seeds, while ahk3 mutant seeds have a very low amount of tannins but elevated levels of other compounds such as sinapoylated glucosinolates (GSLs), important for plant defense. The metabolic profile performed further supports a link between cytokinin and the regulation of secondary metabolites such as flavonoids and glucosinolates. Our results suggest that each cytokinin receptor independently contributes to this regulation, reflected in the distinct metabolic profiles of each mutant.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"59"},"PeriodicalIF":3.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies unveil candidate genes linked with fruit fiber traits in Calotropis procera.","authors":"Hari Shankar Gadri, Vikas Sharma, Mohammed Asif Chowdhary, Sarbani Roy, Rohini Dwivedi, Pankaj Bhardwaj","doi":"10.1007/s00425-025-04781-3","DOIUrl":"10.1007/s00425-025-04781-3","url":null,"abstract":"<p><p>Calotropis procera is a vital emerging natural fiber crop exhibiting unique domestication traits, making it an ideal candidate for widespread cultivation. Fiber phenotypes across 32 populations from arid and barren environments were assessed to determine their quality. The soil physicochemical analysis of both ecological conditions revealed that fibers were more pronounced in the arid zone than in the barren zone, with nitrogen, phosphorus, and potassium having a negative impact. At the same time, total organic carbon reflected a positive correlation with its phenotypes. Genetic variations were investigated through GWAS to identify key alleles and genomic regions underlying potential fiber quality traits. The study uncovered 11,002 high-quality SNPs and 11 significant markers across the genome associated with fiber trait variations. The genomic region (Chr4:14050001_15050000) from cross populations of distinct ecologies was identified under selection, having candidate loci (Chr4:14,869,706) associated with fiber length. The Tajima's D values of these loci were estimated at 0.94 and 0.69 for the arid and barren zones, respectively, indicating differing selection pressures. The region harbors markers potentially shaped by environmental adaptation, offering insights into the genetic basis of fiber trait variation across dynamic ecology. This comprehensive study in C. procera set a new direction for domestication and molecular breeding efforts to enhance desirable fiber traits in arid ecology.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"60"},"PeriodicalIF":3.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of the transcriptomic and metabolic responses of grapevines to arbuscular mycorrhizal fungi.","authors":"Alexis Velásquez, Pablo Cornejo, Marcela Carvajal, Claudio D'Onofrio, Michael Seeger, Italo F Cuneo","doi":"10.1007/s00425-025-04771-5","DOIUrl":"10.1007/s00425-025-04771-5","url":null,"abstract":"<p><strong>Main conclusion: </strong>This review discusses the molecular modifications of grapevines by arbuscular mycorrhizal fungi, increasing anthocyanins and other phenolic molecules, potentially improving wine quality and plant stress tolerance. Grapevines are naturally associated with arbuscular mycorrhizal fungi (AMF). These fungi, as obligate symbionts, are capable of influencing molecular, biochemical, and metabolic pathways, leading to alterations in the concentrations of various molecules within the host plant. Recent studies have addressed the transcriptomic and metabolic modifications triggered by AMF in grapevines. These AMF-induced alterations are involved in cell transport, sugar metabolism, plant defense mechanisms, and increased tolerance to both biotic and abiotic stressors. Notably, the shikimate pathway exhibits heightened activity following AMF inoculation in grapevines, resulting in the accumulation of anthocyanins, flavonols, phenolic acids, and stilbenes. Phenolic compounds are the main metabolites influencing grape and wine quality attributes, such as color, flavor, and potential health benefits. This review aims to provide an updated overview of current research on the transcriptomic and metabolic aspects of AMF-grapevine interactions, focusing on their impact on plant performance and quality traits.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"58"},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt stress-responsive transcription factors provide insights to enhance barley improvement: a review.","authors":"Tayachew Admas, Jiao Shu, Abdullah Shalmani, Rui Pan, Wenying Zhang","doi":"10.1007/s00425-025-04760-8","DOIUrl":"10.1007/s00425-025-04760-8","url":null,"abstract":"<p><strong>Main conclusion: </strong>Salt stress is limiting barley growth, development, and production. Transcription factors (TFs) play a critical role in plant responses to salt stress by modulating gene expression Salinity stress increases over time due to climate change. It represents a major constraint to barley growth, development, and yield. Enhancing salt tolerance to withstand salt stress is crucial for ensuring global food security. We discussed transcription factors (TFs) that play a key role in responding to salt stress by modulating downstream genes and facilitating alterations in physiological and biochemical pathways. TF families strongly associated with the bZIP, DREB, NAC, bHLH, MYB, ERF, and WRKY are particularly involved in regulating ion homeostasis, osmotic adjustment, and signaling under stressful conditions. These discoveries establish a platform for generating salt-tolerant barley genotypes utilizing modern biotechnological methods. CRISPR/Cas and virus-induced gene silencing (VIGS) are broadly used tools to investigate gene function by knocking out or silencing target genes under salt stress. Furthermore, integrating the existing knowledge and foundations of TFs could yield sustainable, salt-resistant barley genotypes for agriculture. Multi-omics and bioinformatics have accelerated the identification of salt-responsive genes and TFs. The review recorded the recent progress in the molecular mechanisms of salinity tolerance in barley and indicates the potential of biotechnology for improving salt tolerance in barley varieties.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"56"},"PeriodicalIF":3.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyssopus officinalis: MicroRNA-mediated regulation of terpenoid biosynthesis and defense metabolites.","authors":"Mojgan Gholami Malekroudi, Mohammad Reza Naghavi","doi":"10.1007/s00425-025-04776-0","DOIUrl":"10.1007/s00425-025-04776-0","url":null,"abstract":"<p><strong>Main conclusion: </strong>This study provides an experimental identification of miRNAs in Hyssop and a prediction of mRNA targets with an eye toward miRNA-mediated regulation of terpenoid biosynthesis. This can help to design future metabolic engineering attempts to improve the yield and quality of source species and unravel its therapeutic potential. MicroRNAs (miRNAs) are important post-transcriptional regulators of plant and animal gene expression. They bind to ARGONAUTE (AGO) proteins to form RNA induced silencing complexes (RISCs) and use base pairing to guide RISC to complementary mRNA for repression. The Lamiaceae family is a rich resource of terpenoids with important commercial and pharmaceutical uses, but little is known about miRNA-mediated control of terpenoid biosynthesis in these species. Here, we applied Trans-kingdom, rapid, affordable purification of RISCs coupled to small RNA sequencing (TraPR-seq) to identify AGO-bound small RNAs in Hyssop (Hyssopus officinalis L.), an important medicinal herb. We identify 15 conserved and 42 novel miRNA families, of which 8 were predicted to be of potential relevance to terpenoid biosynthesis. We also uncover several plausible miRNA targets encoding innate immunity regulators and identify one miRNA as a possible master regulator of defense with targets not only in terpenoid biosynthesis, but also including typical defense regulators such as receptor-like kinases and WRKY transcription factors, and its role in defense responses was boosted by its identification as a confident trigger of phasiRNA production. Our study provides the basis for future exploitation of miRNA regulatory networks for production of valuable terpenoids in Hyssop and gives insight into how miRNAs may coordinately control the expression of defense regulators and defense metabolite production.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"57"},"PeriodicalIF":3.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arabidopsis phytyl ester synthases PES1 and PES2 moonlighted as xanthophyll acyltransferases in plants.","authors":"Jingwei Fu, Katie D Heiden, Laura S Bailey, Kari B Basso, Bala Rathinasabapathi","doi":"10.1007/s00425-025-04765-3","DOIUrl":"10.1007/s00425-025-04765-3","url":null,"abstract":"<p><strong>Main conclusion: </strong>Proteins coded by At1g54570 and At3g25840 previously characterized in the synthesis of phytyl acyl esters also catalyze xanthophyll acyl esterification under tissue-culture-induced stress and when expressed in transgenic tomato. Fatty acyl esters of xanthophylls accumulate in flower petals and ripening fruit of many plant taxa. The genes involved in xanthophyll esterification are, therefore, of interest as xanthophyll esters are known in determining flower and fruit coloration and the accumulation and stability of nutritionally valuable carotenoids. Arabidopsis thaliana proteins homologous to tomato xanthophyll acyltransferases PYP1 and PYP1-like coded by At1g54570 (AtPES1) and At3g26840 (AtPES2), respectively, were previously implicated in the synthesis of fatty acid phytyl esters and triacylglycerol. Here, we tested the hypothesis that AtPES1 and AtPES2 proteins could have roles in xanthophyll esterification also. Chromatographic analyses quantifying carotenoids from tissue-cultured callus of WT, pes1, pes2, and the double mutant pes1/pes2 revealed that AtPES1 and AtPES2 have overlapping functions in the synthesis of xanthophyll mono-, di-, and tri-esters. The xanthophyll esters from Arabidopsis callus were further confirmed and identified by LC-MS. Given AtPES1 and AtPES2's homology to PYP1 and PYP1-like in tomato, complementation tests were performed in the tomato mutant, pyp1-1(H7L) impaired for xanthophyll esterification, in which AtPES1 and AtPES2 were overexpressed. Transgenic expression of PES1 restored the dull yellow petal phenotype of pyp1-1(H7L) to bright yellow like that of the wild-type and xanthophyll esters could be identified in their petal extracts. Transgenic expression of AtPES2 had similar, but less effective impact on restoring petal coloration and xanthophyll ester profiles than AtPES1. Together, our results indicate that Arabidopsis AtPES1 and AtPES2 proteins have overlapping functions in the synthesis of xanthophyll acyl esters.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"51"},"PeriodicalIF":3.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resilience of Portulaca plants to environmental stresses and the economic potential of their bioactive compounds.","authors":"Yong Chie Liew, Lucas Wei Tze Lim, Su-Ee Lau, Boon Chin Tan","doi":"10.1007/s00425-025-04767-1","DOIUrl":"10.1007/s00425-025-04767-1","url":null,"abstract":"<p><strong>Main conclusion: </strong>This review highlights the health benefits and environmental potential of Portulaca species, particularly Portulaca oleracea L., and emphasizes addressing challenges in bioactive compound yields and scalability for broader applications. Portulaca species (Portulaca spp.) are globally distributed and rich in bioactive compounds, including alkaloids, flavonoids, betalains, and fatty acids. These compounds exhibit antioxidant, antibacterial, anti-inflammatory, and anticancer activities. Among these species, Portulaca oleracea L. or purslane is notable for its long history in the traditional medicine. Its aqueous extracts have demonstrated anti-inflammatory, antidiabetic, and antioxidant properties, particularly in mitigating oxidative stress and gastrointestinal disorders. In addition, its nutritional profile, rich in omega-3 fatty acids, vitamins, and minerals, is higher than many leafy vegetables. As a genus of succulents, Portulaca is highly adaptable to abiotic stresses like drought, heat, and salinity due to unique physiological mechanisms, such as C₄ and Crassulacean acid metabolism (CAM) photosynthesis. Its versatility extends to various applications, including soil conditioning, livestock feed, phytoremediation, and pest management. Furthermore, Portulaca's ability to hyperaccumulate heavy metals underscores its potential in environmental cleanup. However, challenges, such as low bioactive compound yields, scalability issues, and regulatory considerations, hinder its broader applications. Advances in metabolomics, genomics, and sustainable cultivation practices are pivotal to unlocking the full potential of Portulaca in agriculture, medicine, and environmental sustainability. This review highlights the untapped potential of Portulaca in agricultural applications, emphasizing its role in developing climate-resilient crops and innovative therapeutic solutions while also exploring the chemical diversity and biological activities of its bioactive compounds.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"53"},"PeriodicalIF":3.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive genomic and transcriptomic analysis of inositol phosphate-metabolizing enzymes in Euglena gracilis.","authors":"Guangjie Xu, Chengfu Sun","doi":"10.1007/s00425-025-04770-6","DOIUrl":"10.1007/s00425-025-04770-6","url":null,"abstract":"<p><strong>Main conclusion: </strong>Euglena gracilis possesses a dynamic inositol metabolic network, with enzyme expression varying under different growth conditions and stressors, enabling future synthetic engineering. Inositol and its phosphate derivatives play pivotal roles in energy metabolism and cellular signaling. Although inositol was detected in Euglena gracilis (E. gracilis) decades ago, the enzymatic machinery governing its metabolic conversion remains poorly characterized. In this study, we conducted a comprehensive bioinformatic analysis of inositol phosphate-metabolizing enzymes and identified 25 enzyme entries (encompassing 35 genes) in this protist. KEGG pathway mapping revealed an active inositol metabolic network in E. gracilis. Genomic structure analysis demonstrated that most of these genes are present in multiple copies across the genome. By constructing a miniaturized genomic representation of these enzymes, we investigated their transcriptional profiles under various conditions, including photo-, hetero-, and mixotrophic growth, aerobic and anaerobic environments, light and dark exposure, and treatment with ethanol, glucose, or other chemical stressors. Our findings indicate that inositol phosphate-metabolizing enzymes exhibit differential expression and dynamic regulation depending on physiological growth conditions and external stimuli. This study establishes a foundation for future catalytic characterization and synthetic engineering of inositol-related enzymes in E. gracilis.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"262 3","pages":"52"},"PeriodicalIF":3.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}