Exploring the differences in traits and genes between brown cotton and white cotton hybrid offspring (Gossypium hirsutum L.).

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-01-14 DOI:10.1007/s00425-024-04601-0
Long Chen, Yujiang Zhang, Qinghua Li, Xu Sun, Junshan Gao, Dahui Li, Ning Guo
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Abstract

Brown cotton and white cotton are two important raw materials used in the cotton fiber industry. Clarifying the differences in morphology, agronomic traits, and fiber pigments between these varieties can facilitate the implementation of corresponding cultivation and breeding techniques. Therefore, we obtained F2 generation brown cotton plants through hybridization and compared them with their parents. In terms of agronomic traits, plant morphology and leaf shape were similar, but brown cotton presented more villi on the main stem. The first fruiting branch node was within the range of 4-6 cm, and the first fruiting branch node height was greater than that of TM-1, i.e., between 13.25 cm and 22.79 cm, with no difference compared with that of P26. The plant height was greater than that of the parents, and the number of bolls was essentially the same as that in TM-1 and greater than that in P26. The lint percentage and average fiber length were lower in TM-1 than in P26, and the seed index was greater than that in TM-1 and P26. Pigment measurements revealed that the chlorophyll a content in brown cotton during the boll stage was lower than that in white cotton, and the content of proanthocyanidin in the cotton fibers was greater in brown cotton than in white cotton. At 15 days after pollination, the highest content was 159.8 mg/g. To determine the differences in gene expression levels, we conducted transcriptome sequencing. Gene Ontology (GO) analysis revealed that the differentially expressed genes (DEGs) were enriched in pathways related to the cell wall and enzyme activity, whereas Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEGs were enriched in flavonoid synthesis pathways. Transcription factor analysis revealed that the expression of the MYB3 transcription factor (Ghir_D07G002110) was higher in brown cotton, and bioinformatics analysis revealed that this gene has regulatory effects on the CHS, CHI1, and F3H genes.

探讨褐棉与白棉杂交后代性状及基因的差异。
棕棉和白棉是棉纤维工业中使用的两种重要原料。弄清这些品种在形态、农艺性状和纤维色素方面的差异,有助于实施相应的栽培育种技术。因此,我们通过杂交获得了F2代褐棉植株,并与亲本进行了比较。在农艺性状上,棕棉的植株形态和叶形相似,但主茎上的绒毛较多。第一结果枝节高度在4 ~ 6 cm范围内,第一结果枝节高度大于TM-1,在13.25 ~ 22.79 cm之间,与P26无显著差异。株高大于亲本,铃数与TM-1基本相同,大于P26。TM-1的衣分和平均纤维长度低于P26,种子指数高于TM-1和P26。色素测定结果表明,铃期褐棉叶绿素a含量低于白棉,棉纤维中原花青素含量褐棉高于白棉。授粉后15 d含量最高,为159.8 mg/g。为了确定基因表达水平的差异,我们进行了转录组测序。基因本体(GO)分析显示,差异表达基因(DEGs)富集于细胞壁和酶活性相关的途径,而京都基因与基因组百科全书(KEGG)分析显示,差异表达基因(DEGs)富集于类黄酮合成途径。转录因子分析显示MYB3转录因子Ghir_D07G002110在褐棉中表达较高,生物信息学分析显示该基因对CHS、CHI1和F3H基因具有调控作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
文献相关原料
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索莱宝
95% ethanol
索莱宝
calcium carbonate
索莱宝
4-(Dimethylamino acid) cinnamaldehyde (DMACA)
索莱宝
6 mol/L hydrochloric acid solution
索莱宝
proanthocyanidin standard
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80% methanol
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