De novo root regeneration from leaf explant: a mechanistic review of key factors behind cell fate transition.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-01-14 DOI:10.1007/s00425-025-04616-1
Sumeera Asghar, Faisal Hayat, Zimo Zhao, Zhu Zheng, Nida Ghori, Zhang Lu, Yan Li, Chunli Chen
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引用次数: 0

Abstract

Main conclusion: De novo root regeneration (DNRR) involves activation of special cells after wounding, along with the converter cells, reactive oxygen species, ethylene, and jasmonic acid, also playing key roles. An updated DNRR model is presented here with gene regulatory networks. Root formation after tissue injury is a type of plant regeneration known as de novo root regeneration (DNRR). DNRR system has wide applications in agriculture and tissue culture biotechnology. This review summarizes the recent advancements in the DNRR model for the cellular and molecular framework, targeting leaf explant of Arabidopsis and highlighting differences among direct and indirect pathways. Key findings highlight the presence of special cells in leaf explants after wounding, under different time lapses, through single-cell sequencing of the transcriptional landscape. The possible roles of reactive oxygen species (ROS), ethylene, and jasmonic acid are explored in the early establishment of wounding signals (short/long) for auxin biosynthesis, ultimately leading to adventitious root formation. The synergistic manner of 3rd type of special cells along converter and regeneration-competent cells automatically leads towards cell fate transition for auxin flux in regeneration-competent cells. The signaling mechanisms of these suggested special cells need to be further investigated to understand the DNRR mechanistic story entirely, in addition to root-to-root regeneration and stem-to-root regeneration. Meta-analysis of DNRR is also presented for past and future reference.

叶片外植体的新生根再生:细胞命运转变背后关键因素的机制综述。
主要结论:根再生涉及损伤后特殊细胞的激活,转化细胞、活性氧、乙烯和茉莉酸也起着关键作用。一个更新的DNRR模型在这里提出了基因调控网络。组织损伤后的根形成是植物再生的一种类型,称为新生根再生(DNRR)。DNRR系统在农业和组织培养生物技术中有着广泛的应用。本文综述了以拟南芥叶片外植体为靶点的DNRR模型在细胞和分子框架方面的最新进展,并重点介绍了直接途径和间接途径的差异。通过对单细胞转录图谱的测序,主要研究结果强调了叶片外植体在受伤后,在不同的时间间隔内,特殊细胞的存在。探讨了活性氧(ROS)、乙烯和茉莉酸在生长素生物合成的早期损伤信号(短/长)建立过程中可能的作用,最终导致不定根的形成。第三类特殊细胞沿着转化器和再生能力细胞的协同方式自动导致再生能力细胞生长素通量的细胞命运转变。除了根到根再生和茎到根再生外,这些特殊细胞的信号机制还需要进一步研究,以全面了解DNRR的机制。本文还对DNRR进行了meta分析,以供过去和未来参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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