Photochemistry and Photobiology最新文献_第7页

Prolonged exposure to artificial light and carcinogenesis: A systematic review of oncostatic mechanisms associated with melatonin pathways. 长期暴露于人造光和致癌：与褪黑激素途径相关的肿瘤稳态机制的系统综述。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-10-14 DOI: 10.1111/php.70040

Gabriel Barboza, Jêmina Oliveira, Antônio Ferreira, Renan Lopes, Marli Cupertino

{"title":"Prolonged exposure to artificial light and carcinogenesis: A systematic review of oncostatic mechanisms associated with melatonin pathways.","authors":"Gabriel Barboza, Jêmina Oliveira, Antônio Ferreira, Renan Lopes, Marli Cupertino","doi":"10.1111/php.70040","DOIUrl":"10.1111/php.70040","url":null,"abstract":"Light pollution from widespread artificial illumination affects photosensitive organisms, including humans. Prolonged exposure to artificial light at night (ALAN), particularly blue light, is associated with melatonin suppression and circadian disruption, both implicated in carcinogenesis. This systematic review investigated the relationship between extended ALAN exposure and malignant neoplasms, identifying associated cancer types and biological mechanisms. A search was conducted in PubMed/Medline and Scopus using PRISMA guidelines. Original studies evaluating associations between ALAN, light pollution, or blue light and cancer in humans were included. Eighteen studies demonstrated a positive link between ALAN and breast cancer, with mechanisms involving interference in the cell cycle, DNA repair, oxidative stress, and activation of oncogenic pathways. Night-shift work correlated with increased breast cancer risk, reduced melatonin levels, and hormonal dysregulation. Exogenous melatonin showed oncostatic potential, reversing epigenetic changes induced by ALAN and reducing tumor burden. Melatonin suppression may promote tumor progression through circadian gene disruption and hormonal imbalance. While findings support a consistent link between ALAN exposure and oncogenesis-especially breast and prostate cancers-methodological variability and confounding factors, such as genetic predisposition and lifestyle, limit generalization. Further studies are needed to clarify mechanisms and explore preventive strategies, including light pollution control and melatonin-based interventions.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"780-796"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviation of photoaging-associated MMP upregulation, prostanoid biosynthesis, and cell cycle arrest with titanium dioxide, zinc oxide, and inorganic-only (ZnO + TiO₂) sunscreens. 二氧化钛、氧化锌和无机（ZnO + TiO2）防晒霜减轻光老化相关的MMP上调、前列腺素生物合成和细胞周期阻滞。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-10-22 DOI: 10.1111/php.70043

Neil Dominic T Pangilinan, Mohammad Shalbaf, Aline Souza, Bhaven Chavan, Mark A Birch-Machin

{"title":"Alleviation of photoaging-associated MMP upregulation, prostanoid biosynthesis, and cell cycle arrest with titanium dioxide, zinc oxide, and inorganic-only (ZnO + TiO2) sunscreens.","authors":"Neil Dominic T Pangilinan, Mohammad Shalbaf, Aline Souza, Bhaven Chavan, Mark A Birch-Machin","doi":"10.1111/php.70043","DOIUrl":"10.1111/php.70043","url":null,"abstract":"Exposure to UVR is well understood to accelerate symptoms of photoaging such as wrinkling and loss of skin elasticity. Sunscreen formulations containing titanium dioxide (TiO2) and zinc oxide (ZnO) UV filters can therefore be used as an effective photoprotective measure to prevent the induction of signaling pathways in skin that contribute to photoaging. The aim of this study is to provide a broad investigation on the photoprotective impact of TiO2, ZnO, and inorganic-only (ZnO + TiO2) sunscreen formulations in human dermal fibroblasts at a gene and protein level. The study focused on genes involved in UV-only and complete solar light-induced MMP production, prostanoid biosynthesis for inflammation, and cell cycle arrest, as previously identified through RNA-seq analysis. Three inorganic formulations were prepared at commercially applicable active levels and varying particle sizes: (1) F(TiO2 179nm), (2) F(ZnO57nm), and (3) an inorganic-only (ZnO + TiO2) formulation F(ZnO57nm/TiO2 47nm). The three formulations significantly alleviated the irradiation-induced expression of MMP1, MMP3, PTGS1, PTGES, MDM2, CDKN1A, and CCNE2, with the latter most alleviated by up to 77% (p ≤ 0.05). The inorganic-only (ZnO + TiO2) formulation, containing both inorganic UV filters, exhibited the greatest mean or maximum alleviation in 75% of the genes investigated. Protein analyses of MMP1, PTGES, and p21, by immunocytochemistry and Western blot, also showed positive translation of alleviation at a protein level. The study provides further academic and commercial insights on the photoprotective impact of inorganic particles in sunscreens, based on relevant signaling pathways, genes, and proteins that are induced by UV to accelerate photoaging.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"889-913"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145346397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creation of nature-inspired PVDF-HFP@SGCN film catalysts for selective solar formic acid production from CO₂ under solar light. 受自然启发的PVDF-HFP@SGCN薄膜催化剂的创造，用于在太阳光下从二氧化碳中选择性地生产太阳甲酸。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-09-17 DOI: 10.1111/php.70033

Pooja Singh, Sandeep Kumar, Rehana Shahin, Satyam Singh, Shaifali Mishra, Atresh Singh, Alok Kumar Singh, Dilip Kumar Dwivedi, Navneet Kumar Gupta, Dushyant Singh Raghuvanshi, Rajesh K Yadav, Jin OoK Baeg, Ravindra Vikram Singh

{"title":"Creation of nature-inspired PVDF-HFP@SGCN film catalysts for selective solar formic acid production from CO2 under solar light.","authors":"Pooja Singh, Sandeep Kumar, Rehana Shahin, Satyam Singh, Shaifali Mishra, Atresh Singh, Alok Kumar Singh, Dilip Kumar Dwivedi, Navneet Kumar Gupta, Dushyant Singh Raghuvanshi, Rajesh K Yadav, Jin OoK Baeg, Ravindra Vikram Singh","doi":"10.1111/php.70033","DOIUrl":"10.1111/php.70033","url":null,"abstract":"Inspired by natural photosynthesis, the study article offers a novel solution to the problem of creating cost-effective and efficient photocatalysts for CO2 fixation. The subject of study is a sulfur-doped graphitic carbon nitride (SGCN) photocatalyst that is metal-free and has drawn a lot of interest because of its possible uses in energy storage, biomaterials, and photocatalysis. The work presents PVDF-HFP@SGCN film, a novel photocatalyst that is created by grafting poly (vinylidene fluoride-co-hexafluoropropylene) polymer brushes onto SGCN under the influence of visible light. The remarkable properties of film photocatalysts, including their capacity to effectively harvest solar light, their compatibility with the optical bandgap, and their well-structured π-electron channels, which enable efficient charge movement, are leveraged in this method. As a result, the recently developed film photocatalyst shows exceptional effectiveness in fixing CO2, resulting in the generation of formic acid as a solar chemical with green energy. This result emphasizes how crucial the study is to developing renewable energy sources and lowering carbon emissions. Ultimately, the work makes a substantial contribution to the development of photocatalytic materials and emphasizes the possibility of PVDF-HFP@SGCN as a very efficient and adaptable catalyst for solar-driven chemical reactions, especially those involving the fixation of CO2 and the generation of renewable energy.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1034-1043"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photophobotaxis of single-celled and filamentous cyanobacteria. 单细胞和丝状蓝藻的趋光性。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-11-13 DOI: 10.1111/php.70051

Maria Sinetova, Nina Spohrer, David Gabrielyan, Luke Lehmann, Julian Breinich, Dmitry Los, Tilman Lamparter

{"title":"Photophobotaxis of single-celled and filamentous cyanobacteria.","authors":"Maria Sinetova, Nina Spohrer, David Gabrielyan, Luke Lehmann, Julian Breinich, Dmitry Los, Tilman Lamparter","doi":"10.1111/php.70051","DOIUrl":"10.1111/php.70051","url":null,"abstract":"Phototaxis refers to an organism's movement toward a light source, while photophobotaxis involves movement into illuminated regions. Although phototaxis in cyanobacteria has been widely studied, photophobotaxis has been investigated in only a few species. In this study, we examined photophobotaxis of 7 single-celled and 11 filamentous cyanobacterial species, among them 3 Nostocales (filaments with heterocysts) and 5 Oscillatoriales and 1 Desertifiliales member. All single-celled species and all Oscillatoriales/Desertifiliales exhibited photophobotaxis, whereas no evidence of photophobotaxis was found for the Nostocales and two other species. A pilus-free mutant of Synechocystis sp. PCC 6803 did not display this behavior. The photosystem II inhibitor DCMU disrupted photophobotaxis in single-celled and filamentous cyanobacteria at a concentration of 10 μM; only the filamentous Phormidium lacuna (P. lacuna) required 100 μM DCMU for inhibition. This points to PS II as a sensor of photophobotaxis. The widespread occurrence of photophobotaxis aligns with the universality of photosystems. Previous studies on spectral sensitivity and the cyanobacteriochrome PixJ in P. lacuna identified PixJ as a negative regulator of photophobotaxis. In pixJ mutants, light sensitivity was increased compared with the wild-type. Dual-wavelength experiments confirmed that yellow light induces PixJ to downregulate photophobotaxis. Our experiments also show that P. lacuna moves faster in darkness than in light and that a temporal change of light intensity from light to dark can induce a change of movement direction. Both findings support the light trap model which is based on random movement and a change of movement direction at the light-dark border.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1021-1033"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145506280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hardening-off under fluctuating light enhances the photosystem I resistance to excessive fluctuating light in Enhalus acoroides. 波动光淬硬增强了光系统I对过度波动光的抗性。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-08-29 DOI: 10.1111/php.70028

Xingkai Che, Yuchen Ye, Bin Zhou, Haixing Wu, Shuchen Liu, Hu Li, Jianguo Liu, Zhendong Li, Yongfu Li

{"title":"Hardening-off under fluctuating light enhances the photosystem I resistance to excessive fluctuating light in Enhalus acoroides.","authors":"Xingkai Che, Yuchen Ye, Bin Zhou, Haixing Wu, Shuchen Liu, Hu Li, Jianguo Liu, Zhendong Li, Yongfu Li","doi":"10.1111/php.70028","DOIUrl":"10.1111/php.70028","url":null,"abstract":"Seagrass meadows of Enhalus acoroides are rapidly declining. Transplanting artificially cultured seedlings is considered an efficient method of seagrass restoration. However, excessive fluctuating light (eFL) in the wild is highly likely to be a major threat to the survival of transplanted seedlings of E. acoroides. This study demonstrated that eFL in the wild inhibited the development of PSI activity in mature E. acoroides plants but had little effect on PSII activity. To verify the effect of FL on artificially cultured seedlings of E. acoroides, we cultured such seedlings under FL and constant light (CL) indoors. The results showed that the PSI activity of FL-cultured seedlings was lower than that of CL, while the resistance of PSI to eFL was significantly enhanced in FL-cultured seedlings. The increase in PSI resistance of FL-cultured seedlings was attributed to the decrease in active PSII reaction centers, which protected PSI from excessive electrons at the moment when low light turns to high light. This study demonstrated that using FL to cultivate E. acoroides seedlings can improve their adaptability to eFL in the wild, as a possible way to increase the survival rate of transplanted seedlings.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1010-1020"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of 222-nm ultraviolet C irradiation bactericidal effect on the surgical field in a rabbit model. 222nm紫外线C照射对兔手术野杀菌作用的研究。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-09-02 DOI: 10.1111/php.70031

Tomoaki Fukui, Yuya Yamamoto, Kyohei Takase, Keisuke Oe, Kenichi Sawauchi, Ryota Nishida, Takahiro Niikura, Rena Kaigome, Masahiro Sasaki, Toru Koi, Hiroyuki Ohashi, Ryosuke Kuroda

{"title":"Investigation of 222-nm ultraviolet C irradiation bactericidal effect on the surgical field in a rabbit model.","authors":"Tomoaki Fukui, Yuya Yamamoto, Kyohei Takase, Keisuke Oe, Kenichi Sawauchi, Ryota Nishida, Takahiro Niikura, Rena Kaigome, Masahiro Sasaki, Toru Koi, Hiroyuki Ohashi, Ryosuke Kuroda","doi":"10.1111/php.70031","DOIUrl":"10.1111/php.70031","url":null,"abstract":"Ultraviolet C (UV-C) not only has a bactericidal effect, but is also cytotoxic; however, UV-C at a wavelength of 222 nm with a high absorption coefficient for proteins is considered safe. We have previously reported the safety of 222-nm UV-C irradiation in humans and rabbits. This study evaluated the bactericidal effect of 222-nm UV-C irradiation on exposed surgical fields. Sixteen-week-old female rabbits were used, and the exposed area on their backs was sprayed with a bacterial solution from swabs collected from their soles. Three groups were formed based on UV-C irradiation: 500 mJ/cm2 of 222-nm UV-C, 200 mJ/cm2 of 254-nm UV-C, which is commonly used in germicidal lamps, and non-UV-C irradiation. The bacterial colonies were counted after irradiation. Both UV-C groups showed a significant reduction in bacterial colonies compared to the nonirradiated group, with no significant difference between the two UV-C groups. Microbiota analysis identified species that could cause surgical site infections. The results of the study suggest that 500 mJ/cm2 of 222-nm UV-C irradiation effectively reduces bacterial load, with a bactericidal effect comparable to 254-nm UV-C; hence, 222-nm UV-C irradiation is a promising and safe tool for minimizing the risk of surgical site infections.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"845-853"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of photobiomodulation therapy for spinal cord injury: A review. 脊髓损伤光生物调节疗法的优化研究进展。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-11-05 DOI: 10.1111/php.70041

Isabella K M Drew, Alan R Harvey, Vincent P Wallace, Stuart I Hodgetts

{"title":"Optimization of photobiomodulation therapy for spinal cord injury: A review.","authors":"Isabella K M Drew, Alan R Harvey, Vincent P Wallace, Stuart I Hodgetts","doi":"10.1111/php.70041","DOIUrl":"10.1111/php.70041","url":null,"abstract":"Interrelated secondary events occur within days and weeks following a spinal cord injury (SCI), constituting a major hurdle in providing both an effective and affordable treatment for spinal cord repair in that it requires a multifaceted approach. Photobiomodulation (PBM) therapy in the red/near-infrared spectrum holds promising reparative potential; however, there are no consistent or defined parameters for PBM delivery, which may explain the limited number of ongoing clinical trials and less-than-optimal reported outcomes. This review outlines the associated complexities of the secondary cascade after SCI, with insights on how and when red/near-infrared irradiation may alleviate these issues. The primary focus is to discuss limitations within the field that may be inhibiting our ability to characterize optimal guidelines and specifications. Ultimately, this review provides a call for action, as there is an urgent need for consensus and standardization of therapeutic preclinical methodologies if we hope to develop treatment protocols that provide a first-line minimally invasive therapy to (i) minimize injury sequelae and (ii) facilitate spinal cord repair. We recommend establishing a universal method to measure the therapeutic dose of light delivered to an injury site and employing standardized methodologies across all studies to assess the benefits of PBM therapy.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"797-831"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of the larvae of the sea urchin Centrostephanus rodgersii under light and polarization conditions. 光和偏振条件下海胆幼体的响应。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-11-16 DOI: 10.1111/php.70055

Ben Li, Matthew Clements, Paulina Selvakumaraswamy, Zhenxu Yang, Ken-Tye Yong, Maria Byrne, Céline Bœhm

{"title":"Response of the larvae of the sea urchin Centrostephanus rodgersii under light and polarization conditions.","authors":"Ben Li, Matthew Clements, Paulina Selvakumaraswamy, Zhenxu Yang, Ken-Tye Yong, Maria Byrne, Céline Bœhm","doi":"10.1111/php.70055","DOIUrl":"10.1111/php.70055","url":null,"abstract":"In this paper, we investigated the response of the larvae of the sea urchin Centrostephanus rodgersii to unpolarized halogen light, darkness as well as polarized (linear, circular and elliptical) halogen light presented in side view to observation chambers. The larvae exhibited positive phototaxis when exposed to unpolarized, horizontally polarized, elliptically polarized, and circularly polarized light. However, they did not respond to vertically polarized light. In fact, larval swimming behavior after exposure to vertically polarized light was the same as keeping them in the dark. These findings indicate that not only may the larvae of C. rodgersii have photoreceptors capable of detecting light in the visible spectrum but they may also possess the capacity to differentiate between horizontally and vertically polarized light, which might help them navigate. These findings suggest the possibility of aligned photoreceptors in these larvae. In addition, we found that the larvae respond to circularly polarized light. This result is notable as there are few documented cases of circular polarization sensitivity in animals. As they did not appear to have a preference between the left- and right-handed polarizations, one would need to study their photoreceptor cells to determine the mechanism by which they can detect circular polarization. This could pave the way to the development of new polarization detectors based on biological mechanisms.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"961-975"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of preirradiation time of erythrosine + potassium iodide-mediated photodynamic therapy on candidal inhibition and singlet oxygen formation. 红红素+碘化钾介导的光动力治疗辐照前时间对念珠菌抑制和单线态氧形成的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-10-31 DOI: 10.1111/php.70053

Teerasak Damrongrungruang, Natnicha Jariamphan, Nitichaya Pratumtip, Sarosha Boonsriram, Prapassara Sirikarn

{"title":"Effect of preirradiation time of erythrosine + potassium iodide-mediated photodynamic therapy on candidal inhibition and singlet oxygen formation.","authors":"Teerasak Damrongrungruang, Natnicha Jariamphan, Nitichaya Pratumtip, Sarosha Boonsriram, Prapassara Sirikarn","doi":"10.1111/php.70053","DOIUrl":"10.1111/php.70053","url":null,"abstract":"We aimed to compare the anticandidal effect among different preirradiation times, measure singlet oxygen levels, and investigate the correlation between candidal reduction and singlet oxygen formation in erythrosine + KI photodynamic therapy. Candida albicans (ATCC10231) biofilms were treated with 100/200 μM erythrosine + 100 mM KI for 1, 3, or 5 min before light-emitting diode (520 ± 10 nm, 250 mW/cm2, 20 J/cm2) irradiation. Phosphate-buffered saline, and nystatin were negative and positive controls, respectively. Candidal cells were quantified using a drop plate assay. Singlet oxygen was measured using a 9,10-dimethylanthacene probe at 375/436 nm emission/excitation wavelengths. Median candidal counts and singlet oxygen formation were compared using Kruskal-Wallis with Dunn test. A p-value <0.05 was considered significant. Spearman's correlation was used to correlate candidal reduction and singlet oxygen formation. All preirradiation times for 200 μM erythrosine + 100 mM KI decreased candidal cells by 7.59 (IQR = 0.33) log10CFU/mL. At a 1-min preirradiation, this group significantly generated higher singlet oxygen than the positive control (p < 0.001). Singlet oxygen levels correlated mildly with candidal reduction in the lower erythrosine group but not the higher group, likely due to singlet oxygen saturation. A 1-min preirradiation with 200 μM erythrosine + 100 mM KI generates singlet oxygen to inhibit C. albicans biofilms and shows potential for clinical oral candidiasis treatment.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"949-960"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13382920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145422196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic inactivation increases cell death rate on persistent Staphylococcus aureus. 光动力失活增加持久性金黄色葡萄球菌的细胞死亡率。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-07-01 Epub Date: 2025-09-15 DOI: 10.1111/php.70036

Maria Vitória Silva Pereira, Bruna Carolina Corrêa, Vanderlei Salvador Bagnato, Kate Cristina Blanco

{"title":"Photodynamic inactivation increases cell death rate on persistent Staphylococcus aureus.","authors":"Maria Vitória Silva Pereira, Bruna Carolina Corrêa, Vanderlei Salvador Bagnato, Kate Cristina Blanco","doi":"10.1111/php.70036","DOIUrl":"10.1111/php.70036","url":null,"abstract":"Bacterial persistence is characterized by a subpopulation of metabolically dormant cells that exhibit transient tolerance to antibiotics, contributing to chronic and recurrent infections, particularly in Staphylococcus aureus, a pathogen responsible for severe infections. This phenomenon is evidenced by a biphasic killing curve, where an initial rapid decline is followed by a slowed death phase. Photodynamic inactivation (PDI) represents a promising strategy for microbial eradication through the generation of reactive oxygen species (ROS). This study investigated persistence formation in two S. aureus strains and evaluated the effects of PDI using curcumin. Time-kill assays with oxacillin revealed biphasic killing curves, indicative of persistence. Heritability testing confirmed that persistence was not passed on to progeny, supporting its phenotypic nature. PDI was performed using curcumin and blue light (450 nm), resulting in a dose-dependent reduction in bacterial viability. However, populations that survived PDI exhibited tolerance-like behavior, with unchanged MIC values, suggesting that ROS generated during PDI may induce a transient dormant state. Notably, post-PDI time-kill assays conducted after metabolic recovery showed a higher rate of bacterial death, indicating enhanced antibiotic susceptibility. In contrast, methicillin-resistant strains (MRSA) showed limited persistence induction, likely due to enhanced oxidative stress defenses. These are important to the understanding of bacterial physiological states when designing therapeutic strategies. The timing of antibiotic administration relative to PDI treatment plays a crucial role in treatment efficacy, which may be either enhanced or compromised depending on bacterial adaptation and recovery dynamics.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"924-933"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13383041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0