Photochemistry and Photobiology最新文献_第7页

Effect of photodynamic therapy on the expression of toll-like receptor 2, -3, -4, and -8 in the cervical lesions. 光动力治疗对宫颈病变中toll样受体2、-3、-4和-8表达的影响。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-03-26 DOI: 10.1111/php.14093

Alexander Dushkin, Afanasiev Maxim, Svitich Oxana, Afanasiev Stanislav, Grishacheva Tatyana, Kukina Polina, Asmik Avagyan, Irina Dushkina, Elena Biryukova, Akmaral Khangeldi, Alexander Karaulov

{"title":"Effect of photodynamic therapy on the expression of toll-like receptor 2, -3, -4, and -8 in the cervical lesions.","authors":"Alexander Dushkin, Afanasiev Maxim, Svitich Oxana, Afanasiev Stanislav, Grishacheva Tatyana, Kukina Polina, Asmik Avagyan, Irina Dushkina, Elena Biryukova, Akmaral Khangeldi, Alexander Karaulov","doi":"10.1111/php.14093","DOIUrl":"10.1111/php.14093","url":null,"abstract":"Photodynamic therapy (PDT) is a minimally invasive treatment with low systemic toxicity and immunomodulatory effects, increasingly applied in managing HPV-associated cervical lesions. Toll-like receptors (TLRs) are critical in regulating immune responses in cervical pathology, yet their dynamics under PDT remain underexplored. This study investigates the effect of PDT on TLR2, TLR3, TLR4, and TLR8 expression in cervical epithelial cells ex vivo, considering lesion severity. The results reveal that TLR8 exhibited the most significant reduction across all groups 2 h after PDT, with the strongest suppression in patients with invasive cervical cancer. TLR4 expression decreased by 24% in HPV-infected patients and by 71% in those with cervical cancer, highlighting its potential role in modulating the inflammatory microenvironment post-PDT. TLR3 showed hyperexpression in LSIL and suppression in HSIL/CIN III, although changes were not statistically significant (p > 0.05). TLR2 expression remained unchanged, likely due to HPV type variability. These findings demonstrate that PDT effectively reduces hyperexpression of TLR8, TLR4, and TLR3 in early-stage cervical cancer, suggesting their potential as biomarkers for treatment efficacy. Further research incorporating HPV typing and advanced techniques like liquid biopsy is essential to refine our understanding of TLRs in PDT for HPV-associated cervical lesions.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"141-153"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosensitizers as potential culprits for compensatory benefits of aPDT in the treatment of peri-implantitis: Unraveling the fundamental knowledge and bounded clinical applications of the aPDT. 光敏剂作为aPDT治疗种植体周围炎代偿益处的潜在罪魁祸首：揭示aPDT的基础知识和有限的临床应用。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-06-02 DOI: 10.1111/php.14120

Amanda Paino Santana, Gabriel Mulinari-Santos, Marta Maria Alves Pereira, Daniela Moreira Cunha, Mariana Martins Guerreiro, Mark Wainwright, Leticia Helena Theodoro, Erica Dorigatti de Avila, Wirley Gonçalves Assunção

{"title":"Photosensitizers as potential culprits for compensatory benefits of aPDT in the treatment of peri-implantitis: Unraveling the fundamental knowledge and bounded clinical applications of the aPDT.","authors":"Amanda Paino Santana, Gabriel Mulinari-Santos, Marta Maria Alves Pereira, Daniela Moreira Cunha, Mariana Martins Guerreiro, Mark Wainwright, Leticia Helena Theodoro, Erica Dorigatti de Avila, Wirley Gonçalves Assunção","doi":"10.1111/php.14120","DOIUrl":"10.1111/php.14120","url":null,"abstract":"Peri-implantitis is an inflammatory disease triggered by a dysbiotic biofilm on dental implants, leading to bone loss and potential implant failure. Although nonsurgical and surgical treatments can reduce the inflammatory process, the high prevalence of peri-implantitis suggests that these interventions are not always effective in arresting disease progression. This has prompted increased interest in antimicrobial photodynamic therapy (aPDT), which utilizes photosensitizers (PSs) activated by light to target bacterial infections. Despite promising benefits, aPDT has not yet achieved consistently favorable clinical outcomes. It may be due to the limitations of current PSs, including restricted light activation, insufficient tissue penetration, and variable antimicrobial efficacy. Additionally, the stability and selectivity of PSs are crucial for their effectiveness against pathogenic bacteria. Variations in light delivery systems and treatment protocols may also contribute to inconsistent results across studies. This review will address the clinical challenges of aPDT for peri-implantitis, critically analyzing the shortcomings outlined in existing literature. It will further explore the chemical and biological mechanisms of PSs, providing insight into the underlying causes of clinical limitations. Finally, we will discuss potential improvements in PS compounds and treatment protocols that could enhance the therapeutic potential of aPDT, offering new perspectives on its role in managing peri-implantitis.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"4-26"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From molecular mechanisms to clinical applications: A comprehensive review of photobiomodulation in cancer treatment. 从分子机制到临床应用：光生物调节在癌症治疗中的综合综述。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-04-21 DOI: 10.1111/php.14107

Qi Wang, Phil-Sun Oh, Hwan-Jeong Jeong

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Erythemal UV radiation exposure during jogging. 慢跑时暴露在紫外线下的红斑。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-05-06 DOI: 10.1111/php.14114

Alois W Schmalwieser, Hannah Danhel, Susanne S Schmalwieser, Sonja Reimoser, Hanna Rummel, Adrian Loy, Jakob Heydenreich, Philipp Weihs

{"title":"Erythemal UV radiation exposure during jogging.","authors":"Alois W Schmalwieser, Hannah Danhel, Susanne S Schmalwieser, Sonja Reimoser, Hanna Rummel, Adrian Loy, Jakob Heydenreich, Philipp Weihs","doi":"10.1111/php.14114","DOIUrl":"10.1111/php.14114","url":null,"abstract":"Jogging is one of the most popular recreational sport activities over four decades and is done at almost all ages to keep fitness and health. Joggers are exposed to solar UV radiation (UVR) and, due to enhanced heat production by physical activity, body coverage by clothes is reduced. This may imply a health risk due to overexposure. However, little research has been undertaken so far to estimate UVR exposure during jogging. Therefore, UVR exposure was measured at seven body sites during jogging under cloud-free conditions for solar elevations between 20° and 60°. Results show that the top of the shoulder is the most exposed body site by receiving 80% of ambient UVR on average and up to 110% under certain conditions. All other body parts receive up to 55% on average and up to 85% in special cases. This indicates further that monotonous body alignment to the sun holds a higher risk than a frequently alternating alignment. Assuming the longest recommended duration for cardiovascular beneficial jogging of 50 min, photosensitive persons need protection of the shoulders from a UV index of 2 onward on an unvaried path and from a UV index of 3 on an all-directional path. Further, results show that measurements of UVR exposure possess an uncertainty of ±15% including mounting.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"57-66"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Absorption and fluorescence properties and assignments of firefly bioluminescence substrate analog: seMpai. 萤火虫生物发光底物类似物的吸收和荧光性质及分配：seMpai。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-05-26 DOI: 10.1111/php.14121

Masahiro Harada, Tomoya Nakano, Ryohei Ono, Tamotsu Uchiyama, Yoshifumi Noguchi, Ryohei Saito-Moriya, Nobuo Kitada, Shojiro A Maki, Takashi Hirano, Hiroshi Aoyama, Masataka Kobayashi, Hidefumi Akiyama, Ken-Ichiro Kanno, Hideyuki Itabashi, Miyabi Hiyama

{"title":"Absorption and fluorescence properties and assignments of firefly bioluminescence substrate analog: seMpai.","authors":"Masahiro Harada, Tomoya Nakano, Ryohei Ono, Tamotsu Uchiyama, Yoshifumi Noguchi, Ryohei Saito-Moriya, Nobuo Kitada, Shojiro A Maki, Takashi Hirano, Hiroshi Aoyama, Masataka Kobayashi, Hidefumi Akiyama, Ken-Ichiro Kanno, Hideyuki Itabashi, Miyabi Hiyama","doi":"10.1111/php.14121","DOIUrl":"10.1111/php.14121","url":null,"abstract":"Electronic absorption and fluorescence properties of a red-emission firefly luciferin analog, seMpai, which is water-soluble and shows a neutral pH, were revealed by quantitative spectroscopic measurements in aqueous solutions of pH 2-10. They were analyzed by density functional theory (DFT) calculations, time-dependent DFT calculations, and vibrational analyses using the neutral form of seMpai and its conjugate acids and bases. In a pH 8 solution, which is commonly used for firefly bioluminescence, seMpai showed an absorption maximum at 380 nm. From the theoretical absorption spectra and pH dependence of the normalized concentrations of the neutral form of seMpai and its conjugate acids and bases, it was found that the most abundant chemical species at pH 8 is the carboxylate anion, and the 380-nm absorption band was assigned as the <math> <semantics><mrow><mi>π</mi></mrow> </semantics> </math> - <math> <semantics><mrow><mi>π</mi></mrow> </semantics> </math> * transition of this anion. The fluorescence spectrum of seMpai in the pH 8 aqueous solution showed an emission maximum at 534 nm for 380-nm excitation. According to the theoretical pH dependence of the normalized concentrations and theoretical absorption and fluorescence energies of the chemical forms of seMpai, the most reasonable fluorescence pathway at pH 8 is the emission from the first excited singlet state S1 of the carboxylate anion through its excitation.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"237-247"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of phenanthridine-based highly selective "Turn-ON" fluorescence chemosensor for the detection of Ru³⁺ in real-time. 基于非苯胺的高选择性“开启”荧光化学传感器的研制及实时检测Ru3+。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-06-05 DOI: 10.1111/php.14122

Nandhini Karthikeyan, Sathiyanarayanan Kulathu Iyer

{"title":"Development of phenanthridine-based highly selective \"Turn-ON\" fluorescence chemosensor for the detection of Ru3+ in real-time.","authors":"Nandhini Karthikeyan, Sathiyanarayanan Kulathu Iyer","doi":"10.1111/php.14122","DOIUrl":"10.1111/php.14122","url":null,"abstract":"A phenanthridine-based fluorogenic probe 5-(2-(prop-2-yn-1-yloxy)phenyl)-7,8,13,14-tetrahydrodibenzo[a,i]phenanthridine (SAAPH) has been successfully synthesized and characterized through nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) spectral analyses. A low detection limit was attained for identifying Ru3+ at a concentration of 151pM with a minimal reaction time of 10 s. Furthermore, incorporating ethylenediaminetetraacetic acid (EDTA) into SAAPH + Ru3+ rendered SAAPH a reversible sensor. Fascinatingly, the fluorescence signal of SAAPH was Turn-ON by the interaction with Ru3+ resulting in the noticeable emission change from colorless to blue with an emission enhancement fold of 12. The detection mechanism of SAAPH was initiated by an Intramolecular Charge Transfer (ICT), wherein electrons were transferred from the electron-rich phenanthridine moiety containing the propargylic group to the electron-deficient Ru3+. Additionally, paper strips were used for fluorescence detection of Ru3+. Further, Ru3+ was detected in environmental water samples and HeLa cells. Consequently, SAAPH demonstrates a lower detection limit than other fluorescent probes and exhibits strong selectivity and sensitivity toward Ru3+.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"114-126"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototherapies used for treating peri-implant diseases (PIDs). 用于治疗种植体周围疾病（pid）的光疗法。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-09-15 DOI: 10.1111/php.70037

Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli

{"title":"Phototherapies used for treating peri-implant diseases (PIDs).","authors":"Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli","doi":"10.1111/php.70037","DOIUrl":"10.1111/php.70037","url":null,"abstract":"This paper is a highlight of the paper by Shahbazi et al. in this issue of Photochemistry and Photobiology. In that systematic review with meta-analysis study, the authors selected a total of 47 papers for the systematic review, and from those, 14 papers were eligible for the meta-analysis. The papers included were based on the adjunctive treatment of peri-implant diseases (PIDs) by the use of photodynamic therapy (PDT), photobiomodulation (LLLT), hyperlight, and high-power lasers in combination with mechanical debridement (MD) used as routine treatment. The clinical findings promoted by the adjunctive treatment based on the phototherapies over the peri-implant diseases were investigated and demonstrated through the literature retrieved and discussed. Photodynamic therapy, photobiomodulation, hyperlight, and high-power lasers used as adjunctive treatments to the mechanical debridement rendered significant impact on different clinical outcomes for both mucositis and peri-implantitis diseases. Photodynamic therapy was the most common phototherapy used in the different studies, and it was capable of contributing to the improvement of important clinical parameters such as: BL (Bone Loss), BoP (Bleeding on Probing), CAL (Clinical Attachment Loss), and PD (Probing Depth); however, these improvements seem to be restricted to short-term evaluation. Yet, after long-term follow-ups, the combined use of phototherapies seems to be similar to those from the conventional treatment, especially MD alone. Among the adjunctive phototherapies discussed by Shahbazi et al., PDT and LLLT seem to be more realistic to be used in the daily clinical setting, especially because they can be more accessible to the clinicians.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"262-270"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking the Ultraviolet Index to the "shadow rule" for sun protection: A theoretical model applied with a southern hemisphere example. 将紫外线指数与防晒的“阴影规则”联系起来：一个以南半球为例的理论模型。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-05-11 DOI: 10.1111/php.14116

Caradee Y Wright, Nathan J Downs, Thandi Kapwata, David Jean du Preez, Bianca Tod

{"title":"Linking the Ultraviolet Index to the \"shadow rule\" for sun protection: A theoretical model applied with a southern hemisphere example.","authors":"Caradee Y Wright, Nathan J Downs, Thandi Kapwata, David Jean du Preez, Bianca Tod","doi":"10.1111/php.14116","DOIUrl":"10.1111/php.14116","url":null,"abstract":"Personal solar ultraviolet radiation (UVR) exposure has positive and negative impacts on human health. Excess solar UVR exposure can be avoided through safe sun practices such as using sun protection and avoiding unprotected outdoor exposure when solar UVR levels are high. The shadow rule indirectly determines the sun's altitude by observing the length of a person's shadow during the course of the day. When the shadow cast by the sun on a horizontal surface is shorter than the height of the person casting the shadow, the solar UVR intensity is high and is deemed to have more risk. The magnitude of this risk depends on factors such a skin type, sun protection used etc. The UV Index is a standardized measure to describe the intensity of solar UVR with respect to the human action spectrum for sunburn. It is frequently reported in weather forecasts aimed at the public. Here, we demonstrate the potential utility of the shadow rule and how it may be understood in relation to the UV Index in a subtropical southern hemisphere setting. Its use as a simple awareness tool for sun protection in locations where the UV Index is not made public has value in promoting sun exposure awareness and reducing personal exposure risk.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"67-78"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143976305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjunctive photochemical therapy for management of inflammatory peri-implant diseases: A systematic review and meta-analysis. 辅助光化学疗法治疗炎性种植体周围疾病：系统回顾和荟萃分析。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-04-18 DOI: 10.1111/php.14098

Soheil Shahbazi, Saharnaz Esmaeili, Anahita Moscowchi, Reza Amid, Georgios Romanos, Mahdi Kadkhodazadeh

{"title":"Adjunctive photochemical therapy for management of inflammatory peri-implant diseases: A systematic review and meta-analysis.","authors":"Soheil Shahbazi, Saharnaz Esmaeili, Anahita Moscowchi, Reza Amid, Georgios Romanos, Mahdi Kadkhodazadeh","doi":"10.1111/php.14098","DOIUrl":"10.1111/php.14098","url":null,"abstract":"This study aimed to systematically review the data on interventions involving adjunctive photochemical therapy in treating peri-implant mucositis (PIM) and peri-implantitis (PI). The electronic search was conducted through six databases in October 2023, seeking studies relying on any kind of adjunctive photochemical therapy in treating inflammatory peri-implant diseases (PIDs). To showcase the effect size, mean differences along with 95% confidence intervals were utilized. Forty-seven studies were deemed eligible for qualitative synthesis, 14 of which were included in the meta-analysis. Low-level light therapy and photodynamic therapy led to enhanced improvements in clinical and radiographic indices, such as bone level (BL), bleeding on probing (BoP), clinical attachment level (CAL), and probing depth (PD), during the 3-month follow-up. However, the differences in efficacy between treatments incorporating adjunctive photochemical therapy and those relying solely on mechanical debridement, considered the gold standard, lessened over extended follow-up periods of 6, 9, and 12 months. Significant differences were observed when comparing the treatment outcomes following adjunctive photochemical therapy between obese and smoker patients with healthy subjects. Incorporating adjunctive photochemical therapy for PID treatment might improve clinical and radiographic parameters in a short-term follow-up of 3 months, but longer-term benefits appear comparable to routine treatments.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"163-197"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

660-808 nm simultaneous irradiation enhance keratinocyte migration and change the keratin expression: An in vitro study. 660-808 nm同时照射增强角质细胞迁移和改变角蛋白表达：一项体外研究。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2026-01-01 Epub Date: 2025-05-27 DOI: 10.1111/php.14124

Renan Carrasco Zuffo, Anaeliza Figueiredo Dos Santos, Luciana Corrêa

{"title":"660-808 nm simultaneous irradiation enhance keratinocyte migration and change the keratin expression: An in vitro study.","authors":"Renan Carrasco Zuffo, Anaeliza Figueiredo Dos Santos, Luciana Corrêa","doi":"10.1111/php.14124","DOIUrl":"10.1111/php.14124","url":null,"abstract":"This in vitro study aimed to determine if simultaneous irradiation with 660 and 808 nm wavelengths enhances keratinocyte migration and alters keratin expression. Keratinocytes were exposed to either 660 nm or 808 nm irradiation alone, as well as to both wavelengths simultaneously using a laser device with the same energy parameters (2 J, 22.22 J/cm2). Cell viability, migration, and keratin expression (K1, K10, K5, and K14) were assessed in a scratch model assay. After 24 h of PBM, the simultaneous group showed higher cell viability compared with the control and the irradiated groups with one wavelength (660 and 808 nm groups). Simultaneous irradiation also resulted in a smaller scratch area compared with the control and 660 nm groups. The frequency of cells positive for K1/K10 at the wound border was lower after dual irradiation, while cells positive for K5/K14 at the wound periphery were more frequent after simultaneous irradiation. These results suggest a potential increase in the population of less-differentiated keratinocytes following 660-808 nm dual emission. In conclusion, combined irradiation improved cell viability and migration, potentially leading to a shift in keratinocyte differentiation. This dual-wavelength effect may help stimulate the reepithelization process in the tissue repair.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"154-162"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144161351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0