Photochemistry and Photobiology最新文献

{"title":"The photobiomodulation effects of continuous and pulsed blue diode laser on proliferation and osteogenic differentiation of periodontal ligament stem cells.","authors":"Mohammadreza Karimi, Mahsa Sheibani Pour, Ardavan Etemadi, Mohammad Amin Karimi, Mahshid Hodjat, Nasim Chiniforush","doi":"10.1111/php.14104","DOIUrl":"https://doi.org/10.1111/php.14104","url":null,"abstract":"<p><p>This study investigated the photobiomodulation effect of pulsed and continuous blue diode laser on osteogenic differentiation and proliferation of periodontal ligament mesenchymal stem cells. Periodontal Ligament Stem cells were seeded in 96-well plates, and 450 nm blue laser irradiation procedure was performed a day after cell seeding. Each experimental group was divided into two subgroups according to their energy density and irradiation duration: Continuous wave (100 mW, 10s, 2 J/cm² and 100 mW, 20 s, 4 J/cm²) and pulse wave (200 mW, 10 s, 2 J/cm² and 200 mW, 20 s, 4 J/cm² and duty cycle 50% for both). Then, all groups were evaluated with a cell viability test (MTT), cell apoptosis (Annexin V) on the second and fourth days after irradiation, Alizarin Red staining on the 14th day after irradiation based on genes. Real-time PCR was conducted 7 and 14 days after irradiation. GAPD gene primers were used as internal control, and OPN, OCN, ALP, and RUNX2 gene primers were used as tests. The one-way ANOVA statistical analysis revealed that cell proliferation in the continuous-irradiated groups was significantly higher than in pulsed groups. However, there is no significant difference in comparison with the control group. Also, pulsed-irradiated groups demonstrated a higher rate of necrosis. The osteogenic differentiation in the continuous groups was more substantial than in the pulsed and the control groups. In comparison to all other study groups, the group that received continuous mode irradiation at an energy density of 2 J/cm², power of 100 mW, and a radiation time of 10 s exhibited significantly higher numbers of calcified nodules and increased expression of OPN, OCN, and ALP genes (p < 0.05). Overall, treating periodontal ligament stem cells with a continuous blue diode laser and appropriate parameters can enhance their osteogenic differentiation and proliferation, accelerating the regeneration of periodontal tissues.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adjunctive photochemical therapy for management of inflammatory peri-implant diseases: A systematic review and meta-analysis.","authors":"Soheil Shahbazi, Saharnaz Esmaeili, Anahita Moscowchi, Reza Amid, Georgios Romanos, Mahdi Kadkhodazadeh","doi":"10.1111/php.14098","DOIUrl":"https://doi.org/10.1111/php.14098","url":null,"abstract":"<p><p>This study aimed to systematically review the data on interventions involving adjunctive photochemical therapy in treating peri-implant mucositis (PIM) and peri-implantitis (PI). The electronic search was conducted through six databases in October 2023, seeking studies relying on any kind of adjunctive photochemical therapy in treating inflammatory peri-implant diseases (PIDs). To showcase the effect size, mean differences along with 95% confidence intervals were utilized. Forty-seven studies were deemed eligible for qualitative synthesis, 14 of which were included in the meta-analysis. Low-level light therapy and photodynamic therapy led to enhanced improvements in clinical and radiographic indices, such as bone level (BL), bleeding on probing (BoP), clinical attachment level (CAL), and probing depth (PD), during the 3-month follow-up. However, the differences in efficacy between treatments incorporating adjunctive photochemical therapy and those relying solely on mechanical debridement, considered the gold standard, lessened over extended follow-up periods of 6, 9, and 12 months. Significant differences were observed when comparing the treatment outcomes following adjunctive photochemical therapy between obese and smoker patients with healthy subjects. Incorporating adjunctive photochemical therapy for PID treatment might improve clinical and radiographic parameters in a short-term follow-up of 3 months, but longer-term benefits appear comparable to routine treatments.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid metal nanoparticles for enhanced delivery of benzoporphyrin derivative in photodynamic cancer therapy.","authors":"Sumeyra Gokalp, Marvin Xavierselvan, Mohammad Forhad Khan, Ronak Shethia, Sima Khani, Ryan H Riddell, Anna V Krasnoslobodtseva, Srivalleesha Mallidi, Michelle Foster","doi":"10.1111/php.14106","DOIUrl":"https://doi.org/10.1111/php.14106","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) is a targeted cancer treatment offering precise tumor ablation with minimal systemic toxicity. However, its clinical application is constrained by poor solubility, rapid clearance, and inadequate tumor accumulation of photosensitizers (PS). This study presents an innovative liquid metal nanoparticle (LMNP) platform, composed of gallium-indium eutectic alloy (EGaIn), engineered to address these drug delivery challenges in PDT. Using a one-step sonication process, EGaIn nanoparticles are synthesized and functionalized with folic acid (FA) for tumor-specific targeting, beta cyclodextrin (β-CD) for enhanced drug encapsulation, and benzoporphyrin derivative (BPD) as a PS. The inclusion of β-CD significantly improves the BPD loading capacity, achieving a three-fold enhancement (52% vs. 18%) while ensuring nanoparticle stability and sustained drug release. Covalent binding of FA and β-CD to the gallium oxide surface enables effective targeting and biocompatibility. In vitro analyses demonstrate potent PDT efficacy, even with reduced cellular uptake, underscoring the platform's ability to overcome intracellular delivery barriers. This LMNP-based nanoplatform addresses critical PDT limitations, such as suboptimal drug delivery and systemic toxicity, leveraging the unique chemical and physical properties of EGaIn nanoparticles. Its multifunctional design integrates targeted delivery, controlled release, and precise therapeutic activation, representing a promising advancement in the development of effective, personalized cancer treatment strategies.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential cell surface targets in patient-derived cultures toward photoimmunotherapy of high-grade serous ovarian cancer.","authors":"Sudip Timilsina, Anish Raju Amara, Rafay Abu, Bryan Q Spring","doi":"10.1111/php.14091","DOIUrl":"10.1111/php.14091","url":null,"abstract":"<p><p>Tumor-targeted, activatable photoimmunotherapy (taPIT) has shown promise in preclinical models to selectively eliminate drug-resistant micrometastases that evade standard treatments. Moreover, taPIT has the potential to resensitize chemo-resistant tumor cells to chemotherapy, making it a complementary modality for treating recurrent high-grade serous ovarian cancer (HGSOC). However, the established implementation of taPIT relies on the overexpression of EGFR in tumor cells, which is not universally observed in HGSOCs. Motivated by the need to expand taPIT applications beyond EGFR, we conducted mRNA-sequencing and proteomics to identify alternative cell surface targets for taPIT in patient-derived HGSOC cell cultures with weak EGFR expression and lacking expression of other cell surface proteins commonly reported in the literature as overexpressed in ovarian cancers, such as FOLR1 and EpCAM. Our findings highlight TFRC and LRP1 as promising alternative targets. Notably, TFRC was overexpressed in 100% (N = 5) of the patient-derived HGSOC models tested, whereas only 60% of models had high EpCAM expression, suggesting that future larger cohort studies should include TFRC. While this study focuses on target identification, future work will expand the approaches developed here to larger HGSOC biopsy repositories and will also develop and evaluate antibody-photosensitizer conjugates targeting these proteins for taPIT applications.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12258538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous photosensitivity of phototheranostic porphyrin-lipid nanoparticles.","authors":"Michael S Valic, Mark Zheng, Theo Husby, Keegan Guidolin, Axel Sahovaler, Sharon Tzelnick, Wenlei Jiang, Michael Halim, Pamela Schimmer, Abdullah El-Sayes, Chris J Zhang, Tina Ye, Ivan Kosik, Jason L Townson, Kenneth K Ng, Lili Ding, Juan Chen, Jonathan C Irish, Robert Weersink, Brian C Wilson, Gang Zheng","doi":"10.1111/php.14088","DOIUrl":"https://doi.org/10.1111/php.14088","url":null,"abstract":"<p><p>Skin photosensitization is a common challenge following intravenous administration of many photodynamic therapy (PDT) drugs, typically lasting days, weeks, or months in laboratory animals and patients. Symptoms of photosensitivity manifest as erythema and edema on skin exposed to sunlight or bright artificial lighting. Recent efforts using nanocarriers to increase photosensitizer accumulation in tumors have also been shown to reduce skin photosensitivity. We previously developed phototheranostic PORPHYSOME (PS) nanoparticles self-assembled from porphyrin-lipid conjugates and capable of potent anti-tumor PDT. Here, we demonstrate in a nonpigmented rat skin model that PS exhibit less severe and shorter-lasting skin photosensitivity compared with an equivalent drug dose of porfimer sodium (PHO), the canonical first-generation PDT drug. At 2, 4, 8, and 12 days post intravenous injection, depilated skin was exposed to escalating doses of simulated solar light. Light exposure 4 days post-injection showed markedly reduced symptoms of skin photosensitivity with PS than PHO. By Day 8, the minimal dose of light eliciting any kind of skin reaction was significantly higher with PS than PHO, and by Day 12, there was no detectable skin response with PS. These differences were attributed to altered intradermal distribution and faster clearance of PS vs. PHO in rat skin.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stage-specific phenotypic and transcriptional alterations in HaCaT keratinocytes exposed to acute and chronic blue light.","authors":"Paulo Newton Tonolli, Suely Kazue Nagahashi Marie, Sueli Mieko Oba-Shinjo, Leonardo Vinicius Monteiro de Assis, Maurício S Baptista","doi":"10.1111/php.14095","DOIUrl":"https://doi.org/10.1111/php.14095","url":null,"abstract":"<p><p>Despite evidence that visible light (VL) has similar effects on human skin as those of UVA, VL is often viewed as harmless. High SPF sunscreen prevents erythema but can lead to overexposure to UVA and VL, with unknown consequences. To explore the impact of chronic blue light exposure, we irradiated (50 J/cm², λ = 408 nm, three times a week) human immortalized keratinocytes under acute (3 irradiations), intermediate (14 irradiations), and chronic (42 irradiations) blue light exposure, monitoring phenotypic and gene expression changes. Chronically exposed keratinocytes exhibit increased nuclei area, chromatin alterations, higher proliferation, and apoptosis resistance, mirroring the consequences of chronic UVA exposure. While acute exposure upregulated keratinization and downregulated tissue repair and apoptosis genes, chronically exposed cells had upregulated genes involved in energy metabolism and oxidative phosphorylation, and downregulated genes were enriched for immune and inflammatory responses. Specific transcription factors were identified in both the acute and chronic stages, some of which have been associated with UVB exposure. IRF1, EGR1, ELF3, FOSL1, and CENPX, SRF, CEBPB, KLF4 were identified in the acute and chronic stages, respectively. We identified some changes in chronically irradiated keratinocytes similar to malignant transformation, emphasizing the need for further research on the long-term impacts of blue light exposure on human skin.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of TNBC metastatic potential following synergistic photodynamic therapy and chemotherapy.","authors":"Jeanne Duong, Rachel A McGinn, Nashielli Diaz, Rebecca Crawley, Deepti H Srinivasan, Jasmine Kwan, Marvin Xavierselvan, Madeleine J Oudin, Srivalleesha Mallidi","doi":"10.1111/php.14100","DOIUrl":"10.1111/php.14100","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer due to a lack of targetable receptors and a high rate of metastasis. The current standard of care for TNBC involves neoadjuvant chemotherapy, such as doxorubicin (DOX), a systemic intervention that affects both cancerous and healthy growing cells throughout the body. In contrast, photodynamic therapy (PDT) offers a minimally invasive alternative, utilizing photosensitizers like benzoporphyrin derivative (BPD) activated by a specific wavelength of light to induce localized cell death. While PDT, both as a standalone treatment and in combination with chemotherapy, has been shown to enhance treatment efficacy in TNBC, its effects on metastatic potential remain poorly understood. To address this knowledge gap, we utilize in vitro assays characterizing cell morphology and invasion as predictors of in vivo metastasis of human TNBC cell lines (BT-20, MDA-MB-231, LM2) following treatments with BPD-PDT alone and in combination with DOX. In this study, we observed that both treatments administered individually demonstrated high sensitivity in BT-20 cells, moderate sensitivity in LM2 cells, and the lowest sensitivity in MDA-MB-231 cells. Comparatively, we found that the combination treatment is most synergistic in LM2 cells and least in BT-20 cells. To assess metastatic potential, we characterized changes in in vitro cell morphology after administering BPD-PDT alone. We discovered that sublethal doses of BPD-PDT alone reduce 3D single-cell and spheroid invasion in LM2 and BT-20 cells, suggesting a decrease in metastatic potential. In contrast, the combination treatment reduced spheroid invasion and led to a more significant increase in pro-apoptotic factors in LM2 and MDA-MB-231 cells compared to BT-20 cells. Overall, our findings highlight the distinct impacts of BPD-PDT alone and the synergistic effects of combination treatment on metastatic potential in TNBC.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrin targeted photodynamic therapy in patient-derived glioblastoma spheroids.","authors":"Miriam Roberto, Meedie Ali, Ivo Que, Rachele Stefania, Henriette S de Bruijn, Dominic J Robinson, Francesco Blasi, Luca D D'Andrea, Enzo Terreno, Laura Mezzanotte","doi":"10.1111/php.14097","DOIUrl":"https://doi.org/10.1111/php.14097","url":null,"abstract":"<p><p>Glioblastoma multiforme (GBM) is the most aggressive primary brain tumor, with a median overall survival of 14.6 months. GBM is incurable because of its invasive growth. These local invasive cells, most significantly glioblastoma stem cells (GSCs), when left behind, resist standard treatment, and cause almost all recurrences. However, the treatment of these infiltrative margins remains a significant challenge, as there are currently no options to reach these margins safely. Photodynamic therapy (PDT) shows promise as localized treatment option using light-activated compounds that target tumor cells and that generate reactive oxygen species (ROS) to destroy them. Far red light, combined with silicon phthalocyanines, could penetrate deeper making it more effective for reaching cancer cells in the tumor margin without compromise of healthy brain. In this study, we used patient-derived GBM spheroids in vitro as a preclinical model to evaluate a new dual-cRGDfK-silicon phthalocyanine conjugate targeting integrin αvβ3, a protein expressed by GBM cells and vasculature. Targeted PDT was efficient in killing GSC spheroids, showing that the combination of far-red light with more precise targeting can reach the type of cells found in the invasive margin, using silicon phthalocyanine as the photosensitizer.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redefining cancer photodynamic therapy with gold nanoparticles.","authors":"Zoey A Lockwood, Michael R Jirousek, James P Basilion, Clemens Burda","doi":"10.1111/php.14099","DOIUrl":"https://doi.org/10.1111/php.14099","url":null,"abstract":"<p><p>Despite advancements made in treatment options, cancer continues to be one of the leading causes of death worldwide. Photodynamic therapy (PDT) has gained attention as a minimally invasive and highly selective treatment option for cancer. However, challenges due to the hydrophobicity of photosensitizers and their poor tumor selectivity have limited their use in cancer therapy. Recent developments in nanotechnology, particularly the use of gold nanoparticles (AuNPs), help overcome these challenges. AuNPs provide a stable and biocompatible platform to deliver photosensitizers, improving their solubility, stability, and ability to target tumors while reducing side effects. Functionalized AuNPs take advantage of mechanisms like the enhanced permeability and retention (EPR) effect and active targeting, improving reactive oxygen species (ROS) production and overall therapeutic efficacy. This review explores innovations in AuNP-based PDT systems, including ligand-functionalized nanoparticles, bioresponsive coatings, and theranostic approaches that combine imaging with therapy. By delving into important aspects of synthesis, characterization, and functionalization, we show how AuNPs improve the delivery and performance of photosensitizers. For instance, systems functionalized with prostate-specific membrane antigen (PSMA) have shown increased therapeutic precision and efficacy in vivo. These advancements are paving the way for more targeted and safer cancer treatments, establishing AuNP-based PDT as a promising approach for developing highly effective oncological therapies with greater precision and fewer side effects.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced antibacterial effect of blue light in combination with an Amazonian tree sap (Croton lechleri).","authors":"Dieter Manstein, Vanessa Silebi","doi":"10.1111/php.14061","DOIUrl":"https://doi.org/10.1111/php.14061","url":null,"abstract":"<p><p>In the United States, 8.2 million patients suffer from non-healing wounds which are often infected with antibiotic-resistant bacteria. Blue light (BL) and Sangre de Drago (Croton lechleri, SD) have potent mechanisms of antibacterial action through free radical formation and anti-biofilm effect, respectively. The aim of this pilot study was to evaluate the enhanced antibacterial effect of a novel combination treatment consisting of blue light and Sangre de Drago. Preliminary dosimetry measurements for effective SD concentration (5%) and 415-nm blue LED light fluence (125.3 J/cm² with a standard variation of 5 mW) were performed. E. coli K-12 (volume 0.1-mL, concentration 2 × 10⁵CFU/mL) was applied to each of 32 tryptic soy agar (TSA) plates. Inoculated TSA plates were separated into four groups: (1) no treatment (Control), (2) treatment with SD only, (3) treatment with blue light (BL) only, and (4) treatment with both SD and BL. Plates were incubated for 12 h at 37°C. Colony-forming units (CFUs) were analyzed using Image J software and count, size and overal TSA plate coverage were quantified. The median CFU count was highest in the Control group (157.9, interquartile range [IQR]: 112.0-157.9), followed by SD-only (60.5, IQR: 51.6-93.6), BL-only (33.7, IQR: 23.6-45.2), while no bacterial growth was observed in the combination treatment group (0, IQR: 0-0). The median CFU size was largest for control (0.44 mm², IQR: 0.35-0.59 mm²), followed by BL-only (0.28 mm², IQR: 0.19-0.43 mm²) and SD-only (0.16 mm², IQR: 0.11-0.23 mm²). BL-only caused a marked reduction in total CFU count, while the median CFU size was only moderately decreased compared to Control. The significant reduction in CFU count may be due to the bactericidal action of BL on bacteria. Conversely, SD-only caused just a moderate decrease in CFU count but had the largest decrease in median CFU size, indicating a possible strong bacteriostatic mechanism of action by SD. The combination of BL and SD resulted in no bacterial growth. The Bliss independence model demonstrated a Bliss synergy value of 0.04 indicating low synergy between the two treatments, even though its presence was significant (p = 0.001). This initial investigation on the combination treatment using 5% SD and 415-nm BL demonstrates synergy resulting in an enhanced antibacterial effect compared to each treatment alone. Further investigation and validation of these results is required. If validated, this novel combination approach may be translated to clinical practice to help treat chronic wounds infected with antimicrobial-resistant bacteria, using non-traditional antimicrobial agents that bypass the most common bacterial mechanisms of antibiotic resistance.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}