Photochemistry and Photobiology最新文献

{"title":"Nanopore sequencing of DNA photoproducts.","authors":"John-Stephen Taylor","doi":"10.1111/php.70052","DOIUrl":"https://doi.org/10.1111/php.70052","url":null,"abstract":"<p><p>Cyclobutane pyrimidine dimers (CPDs) are the major photoproducts of DNA produced by direct absorption of UV light but can also be produced indirectly by photosensitization and chemiexcitation. Deamination of C-containing CPDs is responsible for the majority of C to T mutations caused by UV, which have been linked to skin cancer. Another frequent mutagenic photoproduct of DNA is the (6-4) photoproduct (64PP). Because of their roles in causing mutations, NextGen sequencing methods have been developed to determine the location and frequency of these photoproducts in chromosomal DNA. All these methods, however, rely on enzyme-coupled methods that can only detect one photoproduct at a time. There is evidence, however, that the 64PP and certain oxidized bases can photosensitize CPD formation to produce compound lesions. We propose that such rare but possibly important compound lesions can be detected by single-molecule sequencing using nanopores. Herein, we show that site-specific TT CPD and 64PP photoproducts cause a large current drop when sequenced by an Oxford Nanotechnologies R10-based sequencing device. Furthermore, we demonstrate that both single and multiple photoproducts can be detected in UVB-irradiated DNA substrates containing T₁₁- and (PuTT)₄Pu-tracts. We also provide a simple 9mer kmer model that can simulate the nanopore current data.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145506346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic priming (PDP) targets platinum resistance from chronic perfluoroalkyl substances (PFAS) exposure in ovarian cancer cells.","authors":"Brittany P Rickard, Meghan M Dillon, Lauren A Sapienza-Lundie, Suzanne E Fenton, Imran Rizvi","doi":"10.1111/php.70048","DOIUrl":"10.1111/php.70048","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) is a photochemistry-based treatment modality that synergizes with traditional agents and can overcome chemoresistance. Eighty percent of ovarian cancer patients develop chemoresistant disease, highlighting the need to identify sources of treatment failure and develop rational combinations. Studies have shown that perfluoroalkyl substances (PFAS) induce chemoresistance in a duration-dependent manner in OVCAR-3 cells. PFAS are widespread drinking water contaminants present in the blood of nearly all Americans. The present study evaluated the ability of photodynamic priming (PDP), a sub-cytotoxic variant of PDT, in combination with chemotherapy to overcome chemoresistance in two OVCAR-3 cell cohorts: PFAS chronically-exposed and outgrown (allowed to \"recover\" from chronic PFAS exposure). Effectiveness of benzoporphyrin derivative- (BPD-) or aminolevulinic acid-induced protoporphyrin IX-PDP (ALA-PpIX-PDP) was assessed in combination with carboplatin and doxorubicin. In PFAS chronically-exposed cells, BPD-PDP + carboplatin reduced survival fraction compared to carboplatin alone. Mitochondrial membrane potential also decreased significantly in both cohorts following ALA-PpIX-PDP-based combinations. PDP + doxorubicin also successfully overcame chemoresistance arising from chronic PFAS exposure but was less effective than PDP + carboplatin. Together, these findings demonstrate the efficacy of PDP-based combinations in overcoming chronic PFAS exposure-induced chemoresistance and should be explored in pre-clinical models of ovarian cancer.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of photobiomodulation therapy for spinal cord injury: A review.","authors":"Isabella K M Drew, Alan R Harvey, Vincent P Wallace, Stuart I Hodgetts","doi":"10.1111/php.70041","DOIUrl":"https://doi.org/10.1111/php.70041","url":null,"abstract":"<p><p>Interrelated secondary events occur within days and weeks following a spinal cord injury (SCI), constituting a major hurdle in providing both an effective and affordable treatment for spinal cord repair in that it requires a multifaceted approach. Photobiomodulation (PBM) therapy in the red/near-infrared spectrum holds promising reparative potential; however, there are no consistent or defined parameters for PBM delivery, which may explain the limited number of ongoing clinical trials and less-than-optimal reported outcomes. This review outlines the associated complexities of the secondary cascade after SCI, with insights on how and when red/near-infrared irradiation may alleviate these issues. The primary focus is to discuss limitations within the field that may be inhibiting our ability to characterize optimal guidelines and specifications. Ultimately, this review provides a call for action, as there is an urgent need for consensus and standardization of therapeutic preclinical methodologies if we hope to develop treatment protocols that provide a first-line minimally invasive therapy to (i) minimize injury sequelae and (ii) facilitate spinal cord repair. We recommend establishing a universal method to measure the therapeutic dose of light delivered to an injury site and employing standardized methodologies across all studies to assess the benefits of PBM therapy.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking acetylcholinesterase inhibitor pesticides in the environment using the rapid transient of chlorophyll fluorescence.","authors":"Ivana López Valiño, Gonzalo Dieguez Gaviola, Virginia Emilse Diz, Graciela Alicia González, María Gabriela Lagorio, Gabriela Beatriz Cordon","doi":"10.1111/php.14080","DOIUrl":"10.1111/php.14080","url":null,"abstract":"<p><p>Several home pesticides are organophosphorus compounds. These compounds inhibit the enzyme acetylcholinesterase, causing harmful effects on the health of biota. Through this research, the usefulness of Glycine max (soybean) and Cichorium intybus (chicory) plants as sentinels of organophosphorus compounds in the environment was successfully tested. Different concentrations of the insecticide chlorpyrifos were tried out. Damage to plants at the photosynthetic apparatus level was evaluated by measuring the high temporal resolution variable chlorophyll fluorescence (OJIP test). Several parameters derived from this test indicated a high level of damage in both species even at the mean dose recommended for use in the field. However, a few parameters did not consistently reflect damage in leaves. A drop in the values of the maximum fluorescence (F_M), the quantum yield of electron transport flux, transport between quinones A and B (ET₀/ABS) and the maximal quantum yield of PSII (TR₀/ABS) could alert us about the presence of organophosphates in the environment. An increase in the dissipated energy flux per reaction center (DI₀/RC) values was also observed. The species showed different sensitivities, with soybean plants being the most sensitive. The OJIP transient thus becomes a valuable rapid, non-destructive tool for biomonitoring this class of pesticides in the environment.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1516-1528"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming CO₂ into formic acid by integrated solar-driven catalyst-enzyme coupled artificial photosynthetic system.","authors":"Ankita Singh, Rajesh K Yadav, Abhishek Kumar Gupta, Chandani Singh, Kanchan Sharma, Shaifali Mishra, Rehana Shahin, Atul P Singh, Krishna Kumar Yadav, Jin-Ook Baeg","doi":"10.1111/php.14069","DOIUrl":"10.1111/php.14069","url":null,"abstract":"<p><p>Photo-biocatalyst coupled systems offer a promising approach for converting solar energy into valuable fuels. The bio-integrated photocatalytic system sets a research benchmark by utilizing green energy for formic acid production, reducing CO₂ emissions, and enhancing selectivity through bio-enzyme incorporation. This bio-photocatalytic are promising solutions for environmental remediation and energy production. This research reports the synthesis and application of a novel metal-free, nitrogen-enriched graphene composite photocatalyst (N_enGCTPP) for artificial photosynthesis. N_enGCTPP was synthesized by covalently coupling tetraphenyl porphyrin tetracarboxylic acid (TPP) with N-doped graphene via a polycondensation pathway. The photogenerated charge separation then facilitates the regeneration of enzymatically active coenzymes (NADH) for formic acid production catalyzed by formate dehydrogenase. The photocatalyst exhibited remarkable performance in photocatalytic regeneration of the coenzyme NADH from NAD⁺ with a high yield of 41.80%, as well as photocatalytic production of formic acid (HCO₂H) as a solar fuel from CO₂ with a yield of 99.12 μM. This innovative artificial photosynthetic system demonstrates an affordable, highly efficient, and selective approach for converting carbon dioxide into valuable solar fuels and regenerating NADH, addressing environmental concerns and contributing to sustainable energy solutions.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1480-1490"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous photosensitivity of phototheranostic porphyrin-lipid nanoparticles.","authors":"Michael S Valic, Mark Zheng, Theo Husby, Keegan Guidolin, Axel Sahovaler, Sharon Tzelnick, Wenlei Jiang, Michael Halim, Pamela Schimmer, Abdullah El-Sayes, Chris J Zhang, Tina Ye, Ivan Kosik, Jason L Townson, Kenneth K Ng, Lili Ding, Juan Chen, Jonathan C Irish, Robert Weersink, Brian C Wilson, Gang Zheng","doi":"10.1111/php.14088","DOIUrl":"10.1111/php.14088","url":null,"abstract":"<p><p>Skin photosensitization is a common challenge following intravenous administration of many photodynamic therapy (PDT) drugs, typically lasting days, weeks, or months in laboratory animals and patients. Symptoms of photosensitivity manifest as erythema and edema on skin exposed to sunlight or bright artificial lighting. Recent efforts using nanocarriers to increase photosensitizer accumulation in tumors have also been shown to reduce skin photosensitivity. We previously developed phototheranostic PORPHYSOME (PS) nanoparticles self-assembled from porphyrin-lipid conjugates and capable of potent anti-tumor PDT. Here, we demonstrate in a nonpigmented rat skin model that PS exhibit less severe and shorter-lasting skin photosensitivity compared with an equivalent drug dose of porfimer sodium (PHO), the canonical first-generation PDT drug. At 2, 4, 8, and 12 days post intravenous injection, depilated skin was exposed to escalating doses of simulated solar light. Light exposure 4 days post-injection showed markedly reduced symptoms of skin photosensitivity with PS than PHO. By Day 8, the minimal dose of light eliciting any kind of skin reaction was significantly higher with PS than PHO, and by Day 12, there was no detectable skin response with PS. These differences were attributed to altered intradermal distribution and faster clearance of PS vs. PHO in rat skin.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1541-1558"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12621080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo protective effects of a phytochemical mixture (Posoqueria latifolia extract + Kaempferol) against UVB-induced radiation damage.","authors":"Silvia Ximena Barrios Martínez, Diego Armando Villamizar Mantilla, Nathaly Hernández-Díaz, Lady Johanna Sierra, Raquel Elvira Ocazionez, Elena E Stashenko, María Pilar Vinardell, Jorge Luis Fuentes","doi":"10.1111/php.14086","DOIUrl":"10.1111/php.14086","url":null,"abstract":"<p><p>Plants may be a source of protective compounds that inhibit injuries caused by overexposure to solar radiation. Present work evaluated the protective effects of a phytochemical mixture based on Posoqueria latifolia extract + Kaempferol against ultraviolet-induced radiation DNA damage in human fibroblasts and mice skin. Cytotoxicity, genotoxicity, and antigenotoxicity of P. latifolia flower extract, Kaempferol, and the mixture of both were studied in MRC5 human fibroblasts using Trypan blue exclusion and Comet assays. Kinetics of DNA damage repair post irradiation, in the presence and absence of the phytochemicals, were also evaluated. The inhibition of UVB-induced erythema and cyclobutene pyrimidine dimers (CPD) by the phytochemicals and the mixture were also investigated in mice skin. We showed that the P. latifolia flower extract, Kaempferol, and the mixture inhibited UVB-induced DNA damage and increased DNA repair mechanisms in human fibroblasts. In addition, these phytochemicals inhibited the UVB-induced erythema and CPD in mice skin. The potential of the studied phytochemicals in human photoprotection was discussed.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1454-1467"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the protective effect of Rosa damascena nanogel on collagenase and elastase activity and UVB-induced dermal damage: A rodent model approach to skin photoaging.","authors":"Negar Ranjbar, Mahmoud Osanloo, Mahdi Nasiri-Ghiri, Ali Zarenezhad, Roghayeh Heiran, Ali Ghanbariasad, Najmeh Namdar, Hiva Alipanah","doi":"10.1111/php.14072","DOIUrl":"10.1111/php.14072","url":null,"abstract":"<p><p>While aging inevitably changes our skin, this complex biological process involves much more than just getting older. As the body's largest organ, the skin constantly safeguards us from harmful environmental pathogens and plays a key role in overall well-being. This study investigated the development and evaluation of a nanogel containing Rosa damascena essential oil for its potential anti-aging properties. The nanogel was prepared from a primary nanoemulsion with a particle size of 86 ± 4 nm. Antioxidant activity, measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, was found to be 76% ± 3%. Furthermore, the nanogel demonstrated superior anti-collagenase and anti-elastase activities (60% ± 2% and 51% ± 0.1%, respectively) compared to the essential oil alone. The antibacterial efficacy of the nanogel was tested against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, revealing potent inhibitory effects. To simulate the aging process, rats were subjected to UVB irradiation on both legs 4 days a week before or after treatment. In vivo studies conducted on mice showed that the nanogel effectively reduced the formation of deep wrinkles in treated groups compared to pretreated ones. These findings suggest that the introduced nanogel, with its antioxidant and antimicrobial properties, holds promise as a natural therapeutic approach for wrinkle treatment.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1443-1453"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143067324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of inactivation efficacy of far-UVC (222 nm) and germicidal UVC (254 nm) radiation against virus-laden aerosols of artificial human saliva.","authors":"Monika, Eeshan Ajay Damle, Kiran Kondabagil, Ambarish Kunwar","doi":"10.1111/php.14062","DOIUrl":"10.1111/php.14062","url":null,"abstract":"<p><p>Virus-laden aerosols play a substantial role in the spread of numerous infectious diseases, particularly in enclosed indoor settings. Ultraviolet-C (UVC) disinfection is known to be a highly efficient method for disinfecting pathogenic airborne viruses. Recent recommendations suggest using far-UVC radiation (222 nm) emitted by KrCl* (krypton-chloride) excimer lamps to disinfect high-risk public spaces due to lower exposure risks than low-pressure (LP) mercury lamps (254 nm). This study experimentally explored the comparative effectiveness of far-UVC (222 nm) and germicidal UVC (254 nm) in inactivating virus-laden aerosols of different protective vector media in an air disinfection chamber. The UVC inactivation performances of individual filtered KrCl* excimer lamp and LP mercury lamp were determined for inactivating the bacteriophages, MS2 (icosahedral and non-enveloped ssRNA virus) and Phi6 (spherical and enveloped dsRNA virus) aerosolized from artificial human saliva or sodium chloride and magnesium sulfate (SM) buffer as a vector media. Disinfection efficacy of filtered KrCl* excimer lamp (222 nm) and LP mercury lamp (254 nm) were evaluated for highly concentrated viral aerosols, which replicate those exhaled from infected individuals and remain suspended in air or deposited on surfaces as fomites. Our results show that using individual filtered KrCl* excimer lamp (222 nm) and LP mercury lamp (254 nm) could greatly accelerate the inactivation of the viral bioaerosols formed from artificial human saliva and SM buffer. In the case of 222 nm exposure, Phi6 exhibited significantly more susceptibility in artificial human saliva than in SM buffer whereas MS2 showed comparable vulnerability in both artificial human saliva and SM buffer. However, in the case of 254 nm exposure, both Phi6 and MS2 demonstrated significantly greater susceptibility in artificial human saliva than in SM buffer. This study offers valuable insights and improves our understanding of the influence of different vector media on UVC disinfection of exhaled virus-laden aerosols in indoor environments. These findings can guide the deployment of UVC devices which could greatly contribute to mitigating the transmission of exhaled bioaerosols in public settings.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1415-1428"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-modified titanium dioxide nanoparticles as a highly efficient photocatalyst for the synthesis of furan-3-carboxylate derivatives.","authors":"Muhammad Saleh Faryabi, Ali Akbari, Mahboobeh Zahedifar","doi":"10.1111/php.14111","DOIUrl":"10.1111/php.14111","url":null,"abstract":"<p><p>A heterogeneous photocatalyst based on copper-modified titanium dioxide nanoparticles has been prepared and characterized. Copper-modified nano-titanium dioxide (Nano-TiO₂@CuO) is an efficient photocatalyst for the synthesis of furan-3-carboxylate derivatives through a radical addition-polar cyclization of β-ketoester derivatives and allyl chloride under the irradiation of blue light. Copper-modified titanium dioxide nanoparticles 20% which show titanium dioxide-copper cooperativity show a significantly better performance with respect to turnover numbers (number of product molecules per catalyst molecule = efficiency). The Nano-TiO₂@CuO catalyst is stable and can be reused multiple times without a significant change in activity. The particle sizes varied in the range of 25-80 nm. High yields of product, environmentally friendly, cost-effective method, and easy work-up are advantages of the present method. All synthesized compounds were characterized by NMR and FT-IR spectroscopy.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1577-1591"},"PeriodicalIF":2.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}