Photochemistry and Photobiology最新文献_第9页

Trials and errors in the realm of photodynamic therapy: Viability and ROS detection. 光动力疗法领域的试验与失误：活力与 ROS 检测

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-08-27 DOI: 10.1111/php.14020

David Kessel

引用次数: 0

Effect of treatment frequency on the efficacy of superhydrophobic antimicrobial photodynamic therapy of periodontitis in a wistar rat model. 超疏水抗菌光动力疗法对wistar大鼠牙周炎模型疗效的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-10-10 DOI: 10.1111/php.14021

Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli, Chathuna Bodahandi, Shoaib Ashraf, Tayyaba Hasan, QianFeng Xu, Alexander Greer, Alan M Lyons

{"title":"Effect of treatment frequency on the efficacy of superhydrophobic antimicrobial photodynamic therapy of periodontitis in a wistar rat model.","authors":"Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli, Chathuna Bodahandi, Shoaib Ashraf, Tayyaba Hasan, QianFeng Xu, Alexander Greer, Alan M Lyons","doi":"10.1111/php.14021","DOIUrl":"10.1111/php.14021","url":null,"abstract":"Superhydrophobic antimicrobial photodynamic therapy (SH-aPDT) is advantageous wherein airborne singlet oxygen (1O2) is delivered from a device tip to kill a biofilm with no photosensitizer exposure and no bacterial selectivity (Gram + or Gram -). For effective treatment of periodontitis, the frequency of treatment as well as the optical light fluence required is not known. Thus, we sought to determine whether single or repeated SH-aPDT treatments would work best in vivo using two fluence values: 60 and 125 J/cm2. We assessed the efficacy of three protocols: single treatment; interval treatments (days 0, 2, and 7); and consecutive treatments (days 0, 1, and 2). After 30 days of evaluation, we found that, SH-aPDT in 3 consecutive treatments significantly decreased Porphyromonas gingivalis levels compared to single and interval SH-aPDT treatments, as well as SRP-chlorhexidine (CHX) controls (p < 0.05). Notably, clinical parameters also improved (p < 0.05), and histological and stereometric analyses revealed that consecutive SH-aPDT treatments were the most effective for promoting healing and reducing inflammation. Our study shows what works best for SH-aPDT, while also demonstrating SH-aPDT advantages to treatment of periodontitis including no bacterial selectivity (Gram + or Gram -) and preventing the development of bacterial resistance.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"592-608"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraviolet radiation inhibits mitochondrial bioenergetics activity. 紫外线辐射会抑制线粒体的生物能活动。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-10-16 DOI: 10.1111/php.14034

Aline S Perez, Natalia M Inada, Natasha F Mezzacappo, Jose D Vollet-Filho, Vanderlei S Bagnato

{"title":"Ultraviolet radiation inhibits mitochondrial bioenergetics activity.","authors":"Aline S Perez, Natalia M Inada, Natasha F Mezzacappo, Jose D Vollet-Filho, Vanderlei S Bagnato","doi":"10.1111/php.14034","DOIUrl":"10.1111/php.14034","url":null,"abstract":"Mitochondria play an important role in cellular function, not only as a major site of adenosine triphosphate (ATP) production but also by regulating energy expenditure, apoptosis signaling, control of the cell cycle, cellular growth, cell differentiation, transportation of metabolites, and production of reactive oxygen species. Interaction with electromagnetic waves can lead to dysregulation or alterations in the patterns of energy activities in the mitochondria. Ultraviolet light (UV) can be found in sunlight and artificial sources, such as lamps. UV radiation can cause damage to DNA, proteins, and lipids. Besides that, UV radiation is largely used in microorganism disinfection. To establish possible alterations in mitochondrial bioenergetics, this study proposes to investigate the UV (at two distinct intervals) effects on isolated mitochondria from mice liver to obtain direct responses and selective permeability of the internal membrane information. UVA-371 and UVC-255 nm lamps were used to irradiate, at different doses varying from 22.5 to 756 mJ/cm2, isolated mitochondria samples. Mitochondrial respiration pathways were investigated by high-resolution respirometry, and possible mitochondrial membrane damages were evaluated by mitochondrial swelling by spectrophotometer analysis. UVC irradiation results (in the higher dose) indicate decrease in 75% of mitochondrial bioenergetics capacity, such as limitation of oxidative phosphorylation in 60% and increased energy dissipation in 30%. Mitochondrial swelling experiments (spectrophotometer) indicated inner membrane damage, and consequently a loss of selective permeability. Direct correlation between irradiation and effect responses was observed, mitochondrial bioenergetics is severely affected by UVC radiation, but (UVA) radiation did not present bioenergetic alterations. These alterations can contribute to improving the knowledge behind the cell death mechanism in disinfection UV light and UV therapy such as phototherapy.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"697-708"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142472454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The (un)known issue with using rose bengal as a standard of singlet oxygen photoproduction. 使用玫瑰红作为单线态氧光生成标准的（未知）问题。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-10-08 DOI: 10.1111/php.14030

Krystian Mokrzyński, Grzegorz Szewczyk

引用次数: 0

Ultraviolet radiation thin film dosimetry: A review of properties and applications. 紫外线辐射薄膜剂量测定：特性和应用综述。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-10-07 DOI: 10.1111/php.14022

Alfio V Parisi, Nathan J Downs, Peter Schouten, Damien P Igoe, Joanna Turner, Abdurazaq Amar, Lisa Wainwright, Adrian Dawes, Harry Butler, Stijn Dekeyser

{"title":"Ultraviolet radiation thin film dosimetry: A review of properties and applications.","authors":"Alfio V Parisi, Nathan J Downs, Peter Schouten, Damien P Igoe, Joanna Turner, Abdurazaq Amar, Lisa Wainwright, Adrian Dawes, Harry Butler, Stijn Dekeyser","doi":"10.1111/php.14022","DOIUrl":"10.1111/php.14022","url":null,"abstract":"Spectroradiometry, radiometry, and dosimetry are employed for the measurement of ultraviolet radiation (UVR) irradiance and non-ionizing exposure. Different types of UVR dosimeter have been developed for measuring personal and environmental UVR exposures since film dosimetry was pioneered in the 1970s. An important type of dosimeter is the thin film variant, which contains materials that undergo changes in optical absorbance when exposed to UVR. These changes can be measured at a specific wavelength using a spectrophotometer. Thin film dosimeters allow UVR exposure measurements on humans at various body sites during daily activities, as well as on plants, animals, and any sites of interest when utilized in a field environment. This review examines the properties and applications of five types of thin film UVR dosimeter that have different dynamic exposure limits and spectral responses. Polysulphone, with a spectral response approximating the human erythema action spectrum, was one of the first materials employed in thin film form for the measurement of UVR exposures up to 1 day, and up to 6 days with an extended dynamic range filter. Polyphenylene oxide has been characterized and employed for personal UVR exposure measurements up to approximately four summer days and has also been used for long-term underwater UVR exposures. Phenothiazine and 8-methoxypsoralen have been reported as suitable for the measurement of longer wavelength UVA exposures. Finally, polyvinyl chloride with an extended dynamic exposure range of over 3 weeks has been shown to have predominantly a spectral response in the UVB and extending up to 340 nm.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"532-545"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12080880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light responses during early day phases of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR (PRR) homologous genes in the moss Physcomitrium patens. 苔藓中昼夜节律时钟相关1 （CCA1）和伪反应调节因子（PRR）同源基因在早期阶段的光响应

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-12-27 DOI: 10.1111/php.14047

Katsuhiro Chiso, Takafumi Yamashino, Ryo Suzuki, Tanja Gans, Silvia Trogu, Jon Hughes, Setsuyuki Aoki

{"title":"Light responses during early day phases of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR (PRR) homologous genes in the moss Physcomitrium patens.","authors":"Katsuhiro Chiso, Takafumi Yamashino, Ryo Suzuki, Tanja Gans, Silvia Trogu, Jon Hughes, Setsuyuki Aoki","doi":"10.1111/php.14047","DOIUrl":"10.1111/php.14047","url":null,"abstract":"Circadian clocks facilitate organisms' adaptation to the day-night environmental cycle. Some of the component genes of the clocks (\"clock genes\") respond directly to changes in ambient light, supposedly allowing the clocks to synchronize to and/or oscillate robustly in the environmental cycle. In the dicotyledonous model plant Arabidopsis thaliana, the clock genes CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO-RESPONSE REGULATOR 9 (PRR9) show transient expression in response to the morning light. Here we studied light responses of CCA1a/CCA1b and PRR2, homologous genes to CCA1/LHY and PRR9, respectively, in the moss Physcomitrium patens. We found that light of different wavelengths induced PRR2 while they repressed CCA1a/CCA1b. A disruption strain lacking all phytochrome genes lost PRR2 induction, but still maintained CCA1a/CCA1b repression. The remaining light repression of CCA1a/CCA1b was impaired by the photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Probably therefore, a phytochrome signaling induces PRR2, whereas a photosynthesis-mediated signaling represses CCA1a/CCA1b. Conservation and divergence in the clock gene responses between P. patens and A. thaliana are discussed.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"762-770"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caviar extract inhibits skin photoaging by activating skin stem cells through NF-κB/MMPs/COL17A1 axis. 鱼子酱提取物通过NF-κB/MMPs/COL17A1轴激活皮肤干细胞抑制皮肤光老化。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-12-03 DOI: 10.1111/php.14039

Younan Kou, Wuyan Guo, Yun Wang, Changhua Kou, Bo Zhang

{"title":"Caviar extract inhibits skin photoaging by activating skin stem cells through NF-κB/MMPs/COL17A1 axis.","authors":"Younan Kou, Wuyan Guo, Yun Wang, Changhua Kou, Bo Zhang","doi":"10.1111/php.14039","DOIUrl":"10.1111/php.14039","url":null,"abstract":"Ultraviolet radiations (UVR) produce harmful entities and reactive oxygen species (ROS) in skin cells, leading to skin photoaging. Caviar extract (CE) showed outstanding effects in delaying skin aging, but the underlying mechanism remains largely unknown. In this study, we prepared CE with acid protease and examined the anti-skin photoaging effects. The results showed that CE performed no cytotoxicity to HaCaT cells. For antioxidant properties, the EC50 values of DPPH and ABTS radical scavenging activity for CE were 1.27 and 5.20 mg/mL, respectively. It significantly reduced NF-κB, MMP-3 and MMP-9 protein expression levels, and increased IκB and TIMP-1 expression level in UVA-irradiated HaCaT cells. In the skin aging mice model, CE reduced the degree of UV-induced skin photoaging. Histological study confirmed that CE can ameliorate the adverse effects of UV exposure on the skin. Moreover, we found that CE could enhance the activities of Superoxide dismutase (SOD), and increased the contents of hydroxyproline (HYP) in photoaged mice skin. And CE elevated the protein expression level of COL17A1, KRT10, and KRT14 in mice skin. Taken together, our results bright systemic and new insights of CE into preventing UV-induced skin photoaging.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"683-696"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142771411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of a solar simulator device to standardize microbiological decontamination of contaminated water by solar disinfection by the SODIS and MB/SODIS protocols. 使用太阳能模拟装置，按照 SODIS 和 MB/SODIS 协议，通过太阳能消毒对受污染水体进行标准化微生物净化。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-09-23 DOI: 10.1111/php.14023

Janine Simas Cardoso Rurr, Juliana Patrão de Paiva, Raiane Rosales Diniz, Alvaro Augusto da Costa Leitao, Bryan Hudson Hossy, Nádia Campos de Oliveira Miguel, Claudia de Alencar Santos Lage

{"title":"The use of a solar simulator device to standardize microbiological decontamination of contaminated water by solar disinfection by the SODIS and MB/SODIS protocols.","authors":"Janine Simas Cardoso Rurr, Juliana Patrão de Paiva, Raiane Rosales Diniz, Alvaro Augusto da Costa Leitao, Bryan Hudson Hossy, Nádia Campos de Oliveira Miguel, Claudia de Alencar Santos Lage","doi":"10.1111/php.14023","DOIUrl":"10.1111/php.14023","url":null,"abstract":"Consuming microbiologically-contaminated water is the primary cause of many water-borne diseases and deaths worldwide. Governments aim at providing drinking water for vulnerable populations, especially through low-cost interventions. Therefore, the solar disinfection (SODIS) of such pathogens provides a simple and cost-effective way to obtain good quality water. In this procedure, PET bottles are filled with contaminated water and exposed to sunlight for 1-2 days. To accelerate decontamination, methylene blue (MB) dye added as a photocatalyst, boosts singlet oxygen generation upon absorbing red-band sunlight. This study explores the use of a Sunlight Simulator (SSL) device to research and standardize the SODIS method with a vital dye as MB. PET bottles were filled with artificially-contaminated water with Streptococcus epidermidis and Deinococcus radiodurans Gram-positive bacteria, Escherichia coli and Salmonella typhimurium Gram-negative bacteria, or bacteriophage λ as well. In all experiments, 50 ng/mL MB ensued a synergistic lethal effect after SSL exposure. The results indicate that bacterial and bacteriophage inactivation can be achieved in shorter times with MB-SSL treatment compared to SSL without MB. In this sense, when compared to previous sunlight-SODIS results, the SSL source is a reliable tool to study the parameters of both SODIS and MB-SODIS protocols, and also a feasible tool to afford assays whenever there are unfavorable climate conditions.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"609-618"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142293220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photobiological response of mast cells to green and red light-emitting diodes (LEDs) in cutaneous burns. 皮肤烧伤中肥大细胞对绿色和红色发光二极管（LED）的光生物反应。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 Epub Date: 2024-09-25 DOI: 10.1111/php.14026

Rhuan Isllan Dos Santos Gonçalves, José de Alencar Fernandes Neto, Joabe Dos Santos Pereira, Cassiano Francisco Weege Nonaka, Maria Helena Chaves de Vasconcelos Catão

{"title":"Photobiological response of mast cells to green and red light-emitting diodes (LEDs) in cutaneous burns.","authors":"Rhuan Isllan Dos Santos Gonçalves, José de Alencar Fernandes Neto, Joabe Dos Santos Pereira, Cassiano Francisco Weege Nonaka, Maria Helena Chaves de Vasconcelos Catão","doi":"10.1111/php.14026","DOIUrl":"10.1111/php.14026","url":null,"abstract":"This study assessed the effects of red and green LEDs on mast cells (MCs) in third-degree burns in 75 Wistar rats, divided into control, red LED (RED), and green LED (GREEN) groups. Animals were irradiated daily with RED (630 nm, 300 mW, 0.779 W/cm2, 9 J/cm2, 30 s) and GREEN (520 nm, 180 mW, 0.467 W/cm2, 60 J/cm2, 30 s). Histological sections stained with toluidine blue were analyzed for total and subtype MCs. Standardized MC counting was performed across the viable lesion area, considering lesion margins, through intact connective tissue and the integrity of skin appendages. No statistically significant differences in MCs 2 (with released granules and intact cell border) were found between groups. Irradiated groups showed increased total MCs at 7, 14, and 21 days (p < 0.05), with a decrease in MCs 1 (intact MCs) at all time points compared to control (p < 0.05). Significant changes in MCs 3 (with massive degranulation and partial or complete disintegration of the cell border) degranulation were noted in RED at 7, 14, and 21 days (p < 0.009) and in GREEN at 14 (p < 0.009) and 32 days (p < 0.028). Results suggest red and green LEDs modulate MC recruitment and degranulation in third-degree burns.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"627-635"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142352040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial (2025, issue 3, May/June). 社论（2025年5 / 6月第3期）。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-05-01 DOI: 10.1111/php.14112

Alexander Greer

引用次数: 0