Photochemistry and Photobiology最新文献

{"title":"UV-A photodegradation of vanillin in aqueous solutions: Direct photolysis and photosensitized degradation.","authors":"Heryerli Fernandez, Leo Niederst, Cécile Joyeux, Xavier Allonas, Carolina Lorente","doi":"10.1111/php.14051","DOIUrl":"10.1111/php.14051","url":null,"abstract":"<p><p>Phototoxicity of skin due to the application of skincare products or fragrances is increasing from day to day, since a lot of natural extracts, with low or no toxicity in the dark, are being used in the skin exposed to solar radiation. In particular, the use of 4-hydroxy-3-methoxy benzaldehyde, known as vanillin, in the pharmaceutical, food, and cosmetic industries has increased in the last years. This compound is generally considered as safe for humans, and it has been used in a wide range of applications. Significant beneficial properties, such as antioxidant, anticancer, antimicrobial, among others, have been described for vanillin, along with low toxicity. However, although vanillin is used as an ingredient in cosmetic formulations that are applied to the skin, there are few studies on the photochemistry of vanillin degradation. The degradation of vanillin in aqueous solutions exposed to UV-A radiation (365 nm), both by direct absorption and by means of a photosensitized mechanism, was evaluated in different experimental conditions. On the bases of the experimental results, direct photodegradation of vanillin seems unlikely; however, photosensitized degradation of vanillin was observed in the presence of pterin, and vanillin dimers (6,6-dihydroxy-5,5- dimethoxy-[1,1-biphenyl]-3,3-dicarboxaldehyde) were detected as products. The mechanistic analysis indicates that the long-lived pterin triplet excited state is responsible of vanillin degradation, while the contribution of reactive oxygen species, such as superoxide anion, hydrogen peroxide, or singlet oxygen, is negligible. The thermodynamic feasibility of the potential reactions involved in the degradation mechanism was evaluated with the Rehm-Weller equation. Considering both the thermodynamic and kinetic evidence, we proposed a mechanism for the photosensitized degradation of vanillin.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1049-1060"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The photochemical inheritance of Eduardo Lissi and Juan Grotewold and the intersystem crossings with other inheritances.","authors":"Silvia E Braslavsky, Carlos M Previtali","doi":"10.1111/php.14044","DOIUrl":"10.1111/php.14044","url":null,"abstract":"<p><p>In 1963, Eduardo Lissi and Juan Grotewold started a chemical kinetics and photochemistry group at the School of Sciences at the University of Buenos Aires (Facultad de Ciencias Exactas y Naturales, FCEN, UBA). Political circumstances in Argentina and in Chile were a great determinant of the evolution, dispersion, and re-encounters of the group members. The initial graduate students in the group developed their own research groups working in various Countries and on a variety of projects. We relate the story of the strong interactions with each other of the original group as well as the cooperations and synergy (Intersystem Crossings) of Lissi and Grotewold and of their descendants with other research groups, mainly (but not only) in Latin America. A strong network of partnerships, friendships, and fruitful collaborations between the kineticists, photochemists, and photobiologists in Argentina, Chile and Brazil has evolved from the initial steps in the 1960s.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"790-832"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From femtoseconds to minutes: Spectroscopic study of optically induced thermal diffusion in aqueous solution of rhodamine B.","authors":"René A Nome, Alexandre H Oliveira","doi":"10.1111/php.14042","DOIUrl":"10.1111/php.14042","url":null,"abstract":"<p><p>Given that non-equilibrium molecular motion in thermal gradients is influenced by both solute and solvent, the application of spectroscopic methods that probe each component in a binary mixture can provide insights into the molecular mechanisms of thermal diffusion for a large class of systems. In the present work, we use an all-optical setup whereby near-infrared excitation of the solvent leads to a steady-state thermal gradient in solution, followed by characterization of the non-equilibrium system with electronic spectroscopy, imaging, and intensity. Using rhodamine B in water as a case study, we perform measurements as a function of solute concentration, temperature, wavelength, time, near-infrared laser power, visible excitation wavelength, and isotope effect. We find that spectroscopic excitation of solute and solvent as a function of temperature can be used to understand the thermal diffusion signal. Non-equilibrium molecular dynamics simulations and analysis of infrared spectra and heat diffusion, and stochastic dynamics simulations of the coupled Brownian/spectroscopic non-equilibrium dynamics. The stochastic/spectroscopic model shows how near-infrared excitation of the solvent influences lifetime dynamics on the picosecond timescale as well as Brownian dynamics in real time. Overall, the results presented here exemplify how spectroscopic probing of solute and solvent can be useful for understanding molecular mechanisms of optically induced thermal diffusion in aqueous solution of rhodamine B.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1013-1026"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GOUTERMAN - A module for simulations of tetrapyrrole optical spectra.","authors":"Hai Du, Masahiko Taniguchi, James R Diers, Christine Kirmaier, David F Bocian, Jonathan S Lindsey, Dewey Holten","doi":"10.1111/php.14057","DOIUrl":"10.1111/php.14057","url":null,"abstract":"<p><p>Tetrapyrrole macrocycles comprise the pigments of life (heme, chlorophylls, etc.) and together with synthetic analogues also find wide use in materials chemistry and the life sciences. The distinct colors of the various macrocycles are essential features. A time-honored approach for understanding the electronic spectra entails analyzing the impact of molecular composition on the relative energies of the four frontier molecular orbitals (MOs) of these 18-π annulenes. While first introduced by Prof. Martin Gouterman in 1959, use of the four-orbital model has been largely conceptual. Here, a computational module (GOUTERMAN) is introduced that takes as input the energies of the four MOs, provides user control over key parameters (configuration-interaction energies, dipole strengths, spectral widths), and outputs simulated absorption spectra. The energies of the MOs can be calculated independently (e.g., from density functional theory) or drawn from a small database that is provided. The MO energies and key parameters can be toggled and spectral changes viewed. The GOUTERMAN module is available in PhotochemCAD as a tool for both specialists and non-specialists to visualize, via hands-on experience, the connections between the structural and spectral properties of the tetrapyrrole chromophores found ubiquitously in Nature and used widely in research.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"869-885"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy as a potential approach for preventing fungal spread associated with the use of endotracheal tubes.","authors":"Gabriel Grube Dos Santos, Amanda Cristina Zangirolami, Maria Luiza Ferreira Vicente, Vanderlei Salvador Bagnato, Kate Cristina Blanco","doi":"10.1111/php.14054","DOIUrl":"10.1111/php.14054","url":null,"abstract":"<p><p>Fungal infections related to biofilm formation on medical devices, such as endotracheal tubes (ETTs), pose significant health risks, especially during intubation procedures where fungi like Candida spp. can migrate into the lower respiratory tract. This study explores the use of Photodynamic Therapy (PDT) to prevent fungal cell migration from ETT surfaces to lungs, focusing on the role of curcumin as a photosensitizer. ETTs were coated with varying concentrations of curcumin, and biofilm formation was measured after applying PDT with a 50 J/cm² irradiation dose. The study found that ETTs functionalized with a one-third concentration of CUR reduced biofilm formation by 1.78 Log, significantly lowering microbial load and potentially decreasing hospital-acquired infections. Confocal fluorescence microscopy confirmed that PDT damaged the biofilm's extracellular matrix and caused detachment of dead fungal cells. Moreover, the fluorescence analysis reveals the photodegradation behavior of the photosensitizer within the tube, providing critical insights into its stability and durability, which are essential for evaluating the long-term applicability of these tubes in clinical settings. These results suggest PDT as a promising strategy to reduce fungal infections in high-risk patients, offering potential for future clinical application in preventing device-associated infections.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"858-868"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing bone remodeling and accelerating tooth movement with photobiomodulation in middle-aged adults.","authors":"Selly S Suzuki, Amanda M Ribeiro, Marcella U R Fernandes, Chooryung Chung, Aguinaldo S Garcez","doi":"10.1111/php.14089","DOIUrl":"10.1111/php.14089","url":null,"abstract":"<p><p>Although age is not a contraindication for orthodontic treatment, it can be considered more challenging in mature adult patients, mainly due to a less responsive bone metabolism and reduced osteoblastic activity compared to young patients. In orthodontics, photobiomodulation (PBM) has shown positive results in bone remodeling during tooth movement. This study seeks to assess the effect of PBM can have a positive effect on bone remodeling, stimulating bone formation in middle-aged individuals and allowing for a better periodontal condition. Bone structure and the length of the roots were evaluated using cone beam computed tomography images before and after tooth movement and PBM in patients aged between 40 and 60 years. In addition, we aimed to evaluate the expression of the markers receptor activator of NFκB (RANK), osteoprotegerin (OPG), and osteopontin (OPN) presented in the crevicular fluid using the enzyme-linked immunosorbent assay test. Tooth movement was performed by intruding upper molars with two mini-implants as anchorage bilaterally. One side received PBM with light-emitting diode (LED) light and the contralateral side was used as control (no irradiation). The PBM equipment specially designed for this study consisted of six LEDs of 5 mW each, for 7 minutes, totaling 2 J, 3 times a week, for 5 months. The PMB group showed greater tooth movement (p < 0.05), an increase of 74% on average for bone height. PBM induced a significant increase in RANK expression at month 3 (p < 0.05), and the expression of OPG and OPN showed a significant increase in the fifth month of movement (p < 0.05) in the PBM group. PBM may represent an adjunct therapeutic approach during tooth movement in more mature patients, leading to a more responsive bone metabolism and favoring more comprehensive orthodontic treatment.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"904-912"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation therapy in diabetes: Benefits for pain relief, quality of life, and wound healing.","authors":"Victória Regina da Silva Oliveira, Inaeh de Paula Oliveira, Heloísa Alonso-Matielo, Victhor Teixeira Oliveira, Jean Lucas Kremer, Milena Cristina Dias Casalverini, Fernando Quadros Ribeiro, Silvya Stuchi Maria-Engler, Silvia Romano Assis, Manoel Jacobsen Teixeira, Claudimara Ferini Pacicco Lotfi, José Pinhata Otoch, Camila Squarzoni Dale","doi":"10.1111/php.14053","DOIUrl":"10.1111/php.14053","url":null,"abstract":"<p><p>Globally, 537 million people suffer from diabetes mellitus (DM), a condition often associated with sensory disturbances, wound development, and chronic pain, which significantly affects the quality of life and imposes a substantial economic burden. This study evaluated the effects of photobiomodulation (PBM) therapy on nociceptive and sensory changes in diabetic patients to understand pain manifestations and explore PBM's molecular mechanisms on wound healing. Twenty patients with type 2 DM underwent clinical assessments, completed pain and quality of life questionnaires, and had their pain sensitivity evaluated using the quantitative sensory test (QST). A 5 mm skin biopsy was taken for fibroblast culture. PBM therapy, using 660 nm red light, was administered twice weekly for 7 weeks on lower limb wounds. Results indicated that DM patients faced significant sensory impairments, impacting their quality of life. PBM therapy improved pain scores, alleviated neuropathic pain, and enhanced sensory function, leading to better quality of life and reduced anxiety and depression. It also accelerated wound healing, enhancing mobility and autonomy. In vitro studies showed PBM therapy increased cell proliferation through the ERK signaling pathway and modulation of matrix metalloproteinases (MMP-1/8 and 2) and tissue inhibitors of metalloproteinases (TIMP).</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"913-924"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of the fluorescence of graphene oxide micro-flakes with addition of peripheral benzimidazole group.","authors":"Henrique Luiz Berthault Moura, Marcelo Henrique Gehlen","doi":"10.1111/php.14087","DOIUrl":"10.1111/php.14087","url":null,"abstract":"<p><p>Graphene oxide (GO) is peripherally modified with the addition of 4-(1H-benzimidazol-2-yl) benzaldehyde using a Debus-Radziszewski reaction with its ortho-quinone groups. The GO content of ortho-quinone is enhanced by the oxidation of catechol groups using molybdic acid in a Froehde reaction. Following a Knoevenagel condensation of the peripherally substituted benzimidazole with methyl cyanoacetate, a fluorescent intramolecular charge-transfer (ICT) chromophore is introduced in the GO structure, forming a new fluorescent material called GO-BI (graphene oxide with benzimidazole). Its micro-flake particle has emission concentrated in its border, as revealed by confocal and total internal reflection fluorescence microscopy (TIRFM) images. The emission spectrum of the GO-BI micro-particle is centered on 525 nm and the fluorescence decay is biexponential, with two main components of 1.6 and 7.0 ns when excited at 473 nm.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"979-986"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversal of allodynia and enhanced tendon repair in rats via 660 and 808 nm photobiomodulation.","authors":"Elia Francesco Vedda, Heloísa Alonso Matielo, Mariana Corrêa Sousa, Victhor Teixeira de Oliveira, Victória Regina da Silva Oliveira, Adilson Silva Alves, Luiz Roberto Giorgetti Britto, Camila Squarzoni Dale","doi":"10.1111/php.14082","DOIUrl":"10.1111/php.14082","url":null,"abstract":"<p><p>Our study aimed to explore the potential of Photobiomodulation (PBM) as a treatment for Tendinopathy (TP), a chronic condition characterized by tendon degeneration, inflammation, and pain, for which current treatment options often yield unsatisfactory outcomes. PBM, utilizing low-level laser, has shown promise in reducing inflammatory markers, stimulating cell proliferation, and alleviating pain, thus presenting itself as a promising alternative therapy for TP. Using an experimental rat model of tendinopathy, male Wistar rats weighing 250-370 g were induced with TP through the injection of type I collagenase into the calcaneal tendon. Animals were divided into four equal groups (control, TP, TP + 660 nm, TP + 808 nm), with 10 animals in each group for the nociceptive behavioral tests and 5 in each for the biomolecular analysis. Fifteen days later, PBM was administered to the tendons once daily for seven consecutive sessions using two wavelengths (660 and 808 nm), with 3 J for 30 s, irradiating an area of 0.028 cm² totaling 107.14 J/cm². Mechanical nociception and thermal cold sensitivity were assessed, and tendon samples were collected for analysis of collagen types and total cell count. Spinal cord samples were also collected and immunostained to evaluate specific markers. Our results demonstrated that collagenase injection induced mechanical allodynia and cold hypersensitivity, both of which were reversed by PBM at both 660 and 808 nm wavelengths (**p < 0.005 and *p < 0.05). Additionally, TP led to an increase in collagen type III and a decrease in collagen type I expression, with these effects being reversed by PBM treatment (*p < 0.05). Moreover, TP increased astrocyte immunoreactivity, while PBM at both wavelengths led to a decrease in astrocyte and IL-1β expression. In conclusion, PBM emerged as an effective treatment for TP, as it not only alleviated pain symptoms but also modulated collagen expression and reduced astrocyte and IL-1β expression (**p < 0.01). These findings suggest that PBM could serve as a beneficial adjunctive therapy for analgesia and regeneration in TP management.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"925-935"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemometric modeling of spectroscopic data for characterizing the visible-light-driven photocatalytic N-dealkylation of rhodamine B on a TiO₂ film.","authors":"Jesús M N Morales, Mirta R Alcaraz, Alba Loto, Eduardo A Parellada, Fiorella Tulli, Faustino E Morán Vieyra, Claudio D Borsarelli","doi":"10.1111/php.14043","DOIUrl":"10.1111/php.14043","url":null,"abstract":"<p><p>The green-light driven photocatalytic N-deethylation reaction of Rhodamine B (RhB) on a TiO₂ film was investigated by UV-vis absorption and fluorescence emission spectroscopies, in addition to HPLC and HR-MS, to ascertain the nature of the reaction products. The evolution of the photocatalytic reaction was chemometrically analyzed using Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) of the spectroscopic data to obtain the kinetic and spectral decomposition of the RhB derivatives involved in the reaction. This was then compared with the results obtained by standard HPLC analysis. The MCR-ALS analysis yielded satisfactory spectral and kinetic profiles for RhB and the fully deethylated product, Rhodamine 110. However, the spectral profiles for the N-triethyl (3EtRh) and the mixture of the two isomeric N-diethyl (2EtRh) derivatives exhibited some spectral distortions due to significant spectral overlap between these compounds. In contrast to the HPLC analysis, the MCR-ALS could not resolve the N-ethylrhodamine (EtRh) derivative. The deethylation reactions occurred via independent zero-order steps at the surface of TiO₂, indicating that the RhB degradation reaction is governed by the adsorption-desorption equilibrium of the dye and derivatives on the photocatalyst surface, thereby enhancing the diffusion of compounds on the surface.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1000-1012"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}