Photochemistry and Photobiology最新文献_第8页

The effect of 807-nm light-emitting diode photobiomodulation on peripheral nerve injury. 807纳米发光二极管光生物调节对周围神经损伤的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-11 DOI: 10.1111/php.14090

Biao Chang, Qi Quan, Xinyue Liang, Yanjun Guan, Xing Xiong, Haixia Qiu, Jing Zeng, Yidi Liu, Ying Wang, Jiang Peng, Ying Gu

{"title":"The effect of 807-nm light-emitting diode photobiomodulation on peripheral nerve injury.","authors":"Biao Chang, Qi Quan, Xinyue Liang, Yanjun Guan, Xing Xiong, Haixia Qiu, Jing Zeng, Yidi Liu, Ying Wang, Jiang Peng, Ying Gu","doi":"10.1111/php.14090","DOIUrl":"https://doi.org/10.1111/php.14090","url":null,"abstract":"Photobiomodulation (PBM) has demonstrated potential in promoting peripheral nerve regeneration. However, there is a limited and inconclusive study on the application of light-emitting diode (LED) for nerve injury repair. In this study, we designed an 807-nm LED device with high luminous uniformity to investigate the effects of LED-based PBM on peripheral nerve injury repair. RSC96 cells were utilized as the target cells and optimal light parameters were selected based on ATP concentration, CCK-8 assays, PCR, and immunofluorescent staining. One-way analysis of variance and Student's t-test was used for statistical tests. LED irradiation at 10 mW/cm2 for 30 min effectively enhanced Schwann cell proliferation, neurotrophic factor secretion, and MBP synthesis. To translate the cellular light parameters for animal studies, the transmittance through freshly obtained rat skin and biceps femoris muscle was assessed, and the transmittance was approximately 30%. After directly daily irradiating the sciatic nerve injury area in rats for 21 days at 33 mW/cm2 for 30 min, we used gait analysis, immunofluorescence staining, muscle wet weight ratio, Masson staining, and transmission electron microscopy to evaluate nerve recovery. LED irradiation significantly improved sciatic nerve index, increased MBP staining intensity, and enhanced myelin sheath thickness, nerve diameter, and axon diameter. These results indicated the feasibility of LED-based PBM as a treatment method for peripheral nerve injuries.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic priming overcomes platinum resistance from short-term exposure to select perfluoroalkyl substances in endometrial cancer cell lines. 光动力启动克服了短期暴露于子宫内膜癌细胞系中选择的全氟烷基物质的铂抗性。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-10 DOI: 10.1111/php.14073

Brittany P Rickard, Lauren A Sapienza-Lundie, Marta Overchuk, Xianming Tan, Victoria L Bae-Jump, Melinda S Yates, Suzanne E Fenton, Imran Rizvi

{"title":"Photodynamic priming overcomes platinum resistance from short-term exposure to select perfluoroalkyl substances in endometrial cancer cell lines.","authors":"Brittany P Rickard, Lauren A Sapienza-Lundie, Marta Overchuk, Xianming Tan, Victoria L Bae-Jump, Melinda S Yates, Suzanne E Fenton, Imran Rizvi","doi":"10.1111/php.14073","DOIUrl":"https://doi.org/10.1111/php.14073","url":null,"abstract":"First-line treatment for advanced-stage or recurrent endometrial cancer consists of platinum- and taxane-based chemotherapy, to which many patients will develop resistance. Determining the factors that contribute to platinum resistance and developing alternate treatment options for patients with advanced-stage gynecologic malignancies is critical to improving survival outcomes. Recently, we published the first study evaluating the contribution of perfluoroalkyl substances (PFAS) exposure to platinum resistance in endometrial cancer cell lines and found that select PFAS induce carboplatin resistance, potentially by dysregulating mitochondrial function. The present study expands upon those findings by examining the efficacy of photodynamic priming (PDP) in combination with carboplatin to overcome PFAS-induced platinum resistance. Due to the suspected role of mitochondrial dysfunction in platinum resistance, two clinically approved photosensitizers that, in part, localize to mitochondrial membranes or are synthesized in mitochondria were evaluated: benzoporphyrin derivative (BPD) and aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX), respectively. Combination of ALA-PpIX-mediated PDP + carboplatin resulted in a greater reduction in survival fraction than the same combination with BPD. While PDP with both photosensitizers reduced mitochondrial membrane potential, the reduction was greater with BPD-PDP than ALA-PpIX-PDP. These findings demonstrate that BPD-PDP and ALA-PpIX-PDP in combination with carboplatin can be used to overcome PFAS-induced platinum resistance in endometrial cancer cells.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selectivity and anti-tumor immune elevation by vascular-targeted photodynamic therapy of mouse orthotopic bladder cancer model. 血管靶向光动力治疗小鼠原位膀胱癌模型的选择性及抗肿瘤免疫提升。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-06 DOI: 10.1111/php.14048

Jie Chen, Natalia Kudinova, Rebecca Dubrovsky, Jasmine Thomas, Karan Nagar, Lucas Nogueira, Avigdor Scherz, Kwanghee Kim, Jonathan Coleman

{"title":"Selectivity and anti-tumor immune elevation by vascular-targeted photodynamic therapy of mouse orthotopic bladder cancer model.","authors":"Jie Chen, Natalia Kudinova, Rebecca Dubrovsky, Jasmine Thomas, Karan Nagar, Lucas Nogueira, Avigdor Scherz, Kwanghee Kim, Jonathan Coleman","doi":"10.1111/php.14048","DOIUrl":"https://doi.org/10.1111/php.14048","url":null,"abstract":"Vascular-targeted photodynamic therapy (VTP) with WST11 is a non-surgical tumor ablation approach that is currently being tested in a phase 3 clinical trial for the treatment of upper tract urothelial cancer. WST11-VTP utilizes illumination, leading to hypoxia, and production of free radicals followed by coagulative necrosis. Here, we tested the hypothesis that WST11-VTP can safely ablate muscle-invasive MB-49- luc bladder tumors in an orthotopic mouse model while sparing the surrounding normal tissue. For the safety study, normal mouse bladders were WST11-VTP treated. Fourteen days post-VTP granulomas in local areas around the ablation zone were noticed, which recovered after 44 days. MB49-luc orthotopic tumors at the muscle-invasive stage appeared to be effectively ablated by VTP 4-10 days post-treatment. The anti-tumor response was reflected in the increased invasion of CD4+, CD8+ T cells, myeloid CD11b+ cells, and NK cells in tumor tissue at 7 days post-therapy. Moreover, VTP therapy prolonged the survival of mice bearing orthotopic tumors compared with the untreated control. These results suggest that VTP can selectively ablate malignant tumors in the bladder and promote a robust anti-tumor response in a mouse model that can further augment the therapeutic outcome.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of antimicrobial photodynamic therapy for treatment of breast abscesses: Retrospective Monte Carlo simulation study. 抗菌光动力疗法治疗乳腺脓肿的可行性：回顾性蒙特卡罗模拟研究。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-04 DOI: 10.1111/php.14085

Timothy M Baran, Lam Nguyen, Sophia Palumbo, Anna Weiss, Nebojsa Duric

{"title":"Feasibility of antimicrobial photodynamic therapy for treatment of breast abscesses: Retrospective Monte Carlo simulation study.","authors":"Timothy M Baran, Lam Nguyen, Sophia Palumbo, Anna Weiss, Nebojsa Duric","doi":"10.1111/php.14085","DOIUrl":"10.1111/php.14085","url":null,"abstract":"Breast abscesses are a common issue that affects both breastfeeding and nonpuerperal patients. Even with image-guided drainage and antibiotics, these recur frequently due to unresolved infection. One potential adjunct treatment is photodynamic therapy (PDT), which uses photosensitive compounds to generate cytotoxic reactive oxygen species. Here, we investigate the feasibility of using antimicrobial PDT to treat breast abscesses through a retrospective Monte Carlo simulation study. This study included adult subjects that had ultrasound-guided breast abscess aspiration over a five-year period at our institution (n = 119). Pre-procedure ultrasound images were segmented and used to produce either idealized ellipsoidal volumes or extrapolated segmented volumes for simulated PDT treatment planning. We investigated the effects of abscess wall optical properties and the presence of intra-cavity scattering on our ability to achieve target fluence rates of 4 or 20 mW/cm2 in 95% of the abscess wall. Without intra-cavity scattering, eligibility at the 4 mW/cm2 fluence rate target ranged from 99.2%-100% for ellipsoidal volumes and 76.1%-80.7% for segmented volumes. With intra-cavity scattering added, eligibility at the 4 mW/cm2 target increased significantly to 93.4%-100% for segmented volumes (p < 0.0001). However, this required significantly higher optical power (p < 0.0001). PDT therefore appears to be feasible in this patient population and merits further investigation.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative evaluation of the effects of 635 nm laser on cell proliferation and osteogenic differentiation of buccal fat pad mesenchymal stem cells. 635 纳米激光对颊脂垫间充质干细胞增殖和成骨分化影响的比较评估

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 Epub Date: 2024-06-28 DOI: 10.1111/php.13992

Ardavan Etemadi, Mohammad Amin Karimi, Mohammadreza Karimi, Mahshid Hodjat, Mahsa Sheibani Pour, Alireza Karimi, Nasim Chiniforush

{"title":"A comparative evaluation of the effects of 635 nm laser on cell proliferation and osteogenic differentiation of buccal fat pad mesenchymal stem cells.","authors":"Ardavan Etemadi, Mohammad Amin Karimi, Mohammadreza Karimi, Mahshid Hodjat, Mahsa Sheibani Pour, Alireza Karimi, Nasim Chiniforush","doi":"10.1111/php.13992","DOIUrl":"10.1111/php.13992","url":null,"abstract":"The purpose of this study was to evaluate the effects of 635 nm diode laser with different powers on undifferentiated mesenchymal stem cells obtained from buccal fat pad. Human buccal fat stem cells were cultured in DMEM containing 10% FBS, penicillin, and streptomycin under 5% CO2 and 95% humidity. Cells were cultured in 96-well plate and 24 h later, laser irradiation with 635 nm diode laser was performed in four groups of 200, 300, 400, and 500 mW powers in addition to the control group with the same energy density of 4 J/cm2. MTT and flow cytometry assay was performed to evaluate cell proliferation and viability on 2 and 4 days after irradiation. Alizarin red assay and real-time PCR (OPN, OCN, ALP, and RUNX-2 genes) was performed to evaluate osteogenic differentiation. According to the MTT assay, none of the mentioned powers of 635 nm diode laser had significant effect on cell proliferation. Cells irradiated with power of 400 mW and 500 mW significantly showed a greater number of necrotic cells compared to the control group in Day 4. Cells irradiated with 300 mW power significantly exhibited a greater amount of nodule formation compared to all groups. Results of this study indicated that 635 nm diode laser with energy density of 4 J/cm2 has a positive effect inducing osteogenic differentiation when applying with a power of 300 mW in buccal fat pad mesenchymal stem cells.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"282-289"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling novel targets in melanoma under melanogenesis stimulation and photodynamic therapy by redox proteomics. 通过氧化还原蛋白质组学揭示黑色素瘤在黑色素生成刺激和光动力疗法下的新靶点。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 Epub Date: 2024-07-04 DOI: 10.1111/php.13994

Ester Mazepa, Elizabeth Sousa Cunha, Hellen Paula Valerio, Paolo Di Mascio, Michel Batista, Fabricio Klerynton Marchini, Willian Vanderlei Meira, Guilhermina Rodrigues Noleto, Sheila Maria Brochado Winnischofer, Glaucia Regina Martinez

{"title":"Unveiling novel targets in melanoma under melanogenesis stimulation and photodynamic therapy by redox proteomics.","authors":"Ester Mazepa, Elizabeth Sousa Cunha, Hellen Paula Valerio, Paolo Di Mascio, Michel Batista, Fabricio Klerynton Marchini, Willian Vanderlei Meira, Guilhermina Rodrigues Noleto, Sheila Maria Brochado Winnischofer, Glaucia Regina Martinez","doi":"10.1111/php.13994","DOIUrl":"10.1111/php.13994","url":null,"abstract":"Melanogenesis-stimulated B16-F10 cells enter in a quiescent state, present inhibited mitochondrial respiration and increased reactive oxygen species levels. These alterations suggest that these cells may be under redox signaling, allowing tumor survival. The aim of this study was to evaluate redox-modified proteins in B16-F10 cells after melanogenesis stimulation and rose bengal-photodynamic therapy (RB-PDT). A redox proteomics label-free approach based on the biotin switch assay technique with biotin-HPDP and N-ethylmaleimide was used to assess the thiol-oxidized protein profile. Aconitase was oxidized at Cys-448 and Cys-451, citrate synthase was oxidized at Cys-202 and aspartate aminotransferase (Got2) was oxidized at Cys-272 and Cys-274, exclusively after melanogenesis stimulation. After RB-PDT, only guanine nucleotide-binding protein subunit beta-2-like 1 (Gnb2l1) was oxidized (Cys-168). In contrast, melanogenesis stimulation followed by RB-PDT led to the oxidation of different cysteines in Gnb2l1 (Cys-153 and Cys-249). Besides that, glyceraldehyde-3-phosphate dehydrogenase (Gapdh) presented oxidation at Cys-245, peptidyl-prolyl cis-trans isomerase A (Ppia) was oxidized at Cys-161 and 5,6-dihydroxyindole-2-carboxylic acid oxidase (Tyrp1) was oxidized at Cys-65, Cys-30, and Cys-336 after melanogenesis stimulation followed by RB-PDT. The redox alterations observed in murine melanoma cells and identification of possible target proteins are of great importance to further understand tumor resistance mechanisms.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"302-317"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pyrobacteriopheophorbide-a derivatives possessing various hydrophilic esterifying groups at the C17-propionate residues for photodynamic therapy. 在 C17-丙酸酯残基上具有各种亲水性酯化基团的焦杆菌硫代佛尔酮-a 衍生物，可用于光动力疗法。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 Epub Date: 2024-07-02 DOI: 10.1111/php.13995

Shin-Ichi Sasaki, Yuto Morioka, Kohta Maegawa, Yuya Katsuragi, Takashi Nakamura, Kazuo Kamemura, Hitoshi Tamiaki

{"title":"Pyrobacteriopheophorbide-a derivatives possessing various hydrophilic esterifying groups at the C17-propionate residues for photodynamic therapy.","authors":"Shin-Ichi Sasaki, Yuto Morioka, Kohta Maegawa, Yuya Katsuragi, Takashi Nakamura, Kazuo Kamemura, Hitoshi Tamiaki","doi":"10.1111/php.13995","DOIUrl":"10.1111/php.13995","url":null,"abstract":"Aiming at the application to photodynamic therapy, natural bacteriochlorophyll-a was converted to chemically stable free-base derivatives possessing different kinds of hydrophilic C17-propionate residues. These semi-synthetic bacteriochlorins were found to have self-assembling ability in an aqueous environment and formed stable J-type aggregates in a cell culture medium containing 0.2% DMSO. The electronic absorption spectra of all the sensitizers showed Qy absorption maxima at 754 nm in DMSO as their monomeric states, while a drastic shift of the red-most bands to ca. 880 nm was observed in the aqueous medium. The circular dichroism spectra in the medium showed much intense signals compared to those measured in DMSO, supporting the formation of well-ordered supramolecular structures. By introducing hydrophilic side chains, the bacteriochlorin sensitizers could be dispersed in the aqueous medium as their J-aggregates without the use of any surfactants. Cellular uptake efficiencies as well as photodynamic activities were evaluated using human cervical adenocarcinoma HeLa cells. Among the 11 photosensitizers investigated, the best result was obtained for a charged derivative possessing trimethylammonium terminal (17-CH2CH2COOCH2CH2N+(CH3)3I-) and photocytotoxicity of EC50 = 0.09 μM was achieved by far-red light illumination of 35 J/cm2 from an LED panel (730 nm).","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"318-329"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial (2025, issue 2, March/April). 社论（2025年3 / 4月第2期）。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 DOI: 10.1111/php.14084

Alexander Greer

引用次数: 0

The effect of different parameters of low-level laser used in the treatment of oral mucositis, on the viability and apoptosis of oral squamous cell carcinoma cells: In vitro study. 用于治疗口腔黏膜炎的低强度激光的不同参数对口腔鳞状细胞癌细胞活力和凋亡的影响：体外研究。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 Epub Date: 2024-07-19 DOI: 10.1111/php.13997

Shiva Shirazian, Atieh Mohseni, Sara Pourshahidi, Mojgan Alaeddini, Shahroo Etemad-Moghadam, Mehdi Vatanpour

{"title":"The effect of different parameters of low-level laser used in the treatment of oral mucositis, on the viability and apoptosis of oral squamous cell carcinoma cells: In vitro study.","authors":"Shiva Shirazian, Atieh Mohseni, Sara Pourshahidi, Mojgan Alaeddini, Shahroo Etemad-Moghadam, Mehdi Vatanpour","doi":"10.1111/php.13997","DOIUrl":"10.1111/php.13997","url":null,"abstract":"Oral mucositis is a complication of chemo/radiotherapy. To assess the impact of various power levels of diode-laser on the survival and expression of apoptosis-related genes in oral cancer cells, it is crucial to consider the potential existence of malignant cells within the treatment region and the reliance of laser effectiveness on its specific characteristics. Cal-27 cells were cultivated and exposed to a 660 nm-diode-laser at power levels of 20, 40, and 80 mW, alongside non-irradiated control cells. Viability and expression of Bax and Bcl-2 mRNA were assessed with Methyl Thiazolyl Tetrazolium (MTT) and Real-time Polymerase Chain Reaction (RT-PCR), respectively. The results were analyzed using one-way ANOVA and Tukey post-hoc test (p < 0.05). A significant reduction in viability was found only in the 20 mW group compared to controls (p = 0.001). Cell survival was significantly lower in cells receiving 20 mW laser than those treated with 40 and 80 mW (p < 0.05). None of the laser groups showed significant changes in BcL-2, but Bax was significantly lower in cells receiving 40 and 80 mW (p < 0.05), compared to controls. Laser irradiation at 660 nm (2 J/cm2, 30 s) significantly reduced the viability of oral cancer cells when using 20 mW power. These specifications align with the recommendation that the lowest possible laser dose should be applied for treating cancer patients. The exact mechanism of cell death following laser therapy with these specifications requires further investigation.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"330-337"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interventional human ocular safety experiments for 222-nm far-ultraviolet-C lamp irradiation. 222 纳米远紫外-C 灯照射的介入性人体眼部安全实验。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-01 Epub Date: 2024-08-19 DOI: 10.1111/php.14016

Kazunobu Sugihara, Sachiko Kaidzu, Masahiro Sasaki, Masaki Tanito

{"title":"Interventional human ocular safety experiments for 222-nm far-ultraviolet-C lamp irradiation.","authors":"Kazunobu Sugihara, Sachiko Kaidzu, Masahiro Sasaki, Masaki Tanito","doi":"10.1111/php.14016","DOIUrl":"10.1111/php.14016","url":null,"abstract":"The study aimed to directly assess the ocular safety of 222-nm far-ultraviolet-C (UVC) irradiation in humans, given the limited clinical trials in this area. This wavelength offers the potential for safe and effective microbial inactivation in occupied spaces, but its safety profile for human eyes requires thorough investigation. This prospective, interventional study involved five subjects aged 29-47 years, who were exposed to 222-nm UVC at doses of 22, 50, and 75 mJ/cm2. The subjects were monitored using custom-made glasses with a UV-cut filter on one eye to serve as a control. UVC irradiation was conducted using a KrCl excimer lamp, and ocular examinations were performed prior to exposure, 24 h post-exposure, and at 1, 3, and 6 months. Parameters assessed included visual acuity, refractive error, corneal endothelial density, corneal erosion scores, and conjunctival hyperemia scores. The study found no clinically significant photokeratitis or long-term eye damage across the five subjects, even at the highest dose of 75 mJ/cm2. Temporary ocular discomfort, including sensations of dryness and epiphora, was reported, but these symptoms subsided within hours after irradiation. The findings indicate that 222-nm far-UVC irradiation up to 75 mJ/cm2 does not cause \"clinically significant photokeratitis\" or long-term ocular damage, though it may induce temporary discomfort. This supports the safe use of 222-nm UVC for germicidal applications in occupied environments, providing a basis for revised safety guidelines.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"517-526"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0