Matías I Quindt, Gabriel F Gola, Javier A Ramirez, Sergio M Bonesi
{"title":"Photochemical construction of chromanone-fused estrones using a biphasic tandem rearrangement-cyclization approach.","authors":"Matías I Quindt, Gabriel F Gola, Javier A Ramirez, Sergio M Bonesi","doi":"10.1111/php.70017","DOIUrl":"https://doi.org/10.1111/php.70017","url":null,"abstract":"<p><p>A facile photochemical preparation of 4-chromanone fused to estrone has successfully been achieved upon direct irradiation with light of 254 nm under a nitrogen atmosphere employing 3-(2'-alkenoyl)estrone and 3-(2'-alkenoyl)-17-norestrone derivatives as optimal substrates. The two-phase acid- and base-catalyzed method relies upon two consecutive pathways in a one-pot fashion, involving the photo-Fries rearrangement reaction and a catalyzed intramolecular oxa-Michael addition to afford the desired 4-chromanone fused products in good yields.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Human adipose mesenchymal stem cell derived extracellular vesicles-delivered HSP27 alleviates UVB-induced photoaging.","authors":"Qianwen Zeng, Rentao Yu, Genlong Bai, Qian Wu, Bo Chen, Aijun Chen","doi":"10.1111/php.70015","DOIUrl":"https://doi.org/10.1111/php.70015","url":null,"abstract":"<p><p>Skin photoaging is a skin condition caused by long-term exposure to ultraviolet radiation, especially UVA and UVB, which leads to wrinkles, pigmentation, skin sagging, and telangiectasia. Histopathologically, it is characterized by a significant reduction in dermal collagen and abnormal accumulation of elastic fibers. Preventing or ameliorating photoaging may provide a promising therapeutic approach for these changes. In recent years, multiple studies have reported the potential of mesenchymal stem cells (MSCs) in treating various skin diseases. Given that extracellular vesicles (EVs) can deliver diverse substances to receptor cells and produce therapeutic effects similar to parental cells, we aim to explore whether adipose-derived mesenchymal stem cell-derived extracellular vesicles (AMSC-EVs) can improve skin photoaging by delivering heat shock protein 27 (HSP27). The specific effects of AMSC-EVs on the photoaging model of human dermal fibroblasts (HDFs) or human immortalized keratinocytes (HaCaTs) induced by UVB irradiation were investigated through CCK-8 experiments, cell migration experiments, flow cytometry, immunofluorescence, and Western blot. Our research found that AMSC-EVs improved the survival rate and migration ability of HDFs and HaCaTs after UVB irradiation, alleviated cell senescence, reduced DNA damage, inhibited the production of ROS, and promoted the remodeling of extracellular matrix (ECM). Further research showed that after knocking down HSP27, the anti-aging/light protection ability of AMSC-EVs was significantly weakened. Overall, our data suggest that we have revealed the anti-photoaging effect of AMSC-EVs on HDFs and HaCaTs, which may be mediated by the delivery of HSP27.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144744167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zheng Tang, David Welch, Manuela Buonanno, Mark Gerber, David J Brenner
{"title":"Germicidal potential and skin compatibility of an innovative UVC phototherapy device emitting at 234 nm.","authors":"Zheng Tang, David Welch, Manuela Buonanno, Mark Gerber, David J Brenner","doi":"10.1111/php.70016","DOIUrl":"https://doi.org/10.1111/php.70016","url":null,"abstract":"<p><p>Chronic wounds are a major healthcare issue affecting more than 10 million Americans each year, with a 5-year survival similar to cancer and costing the healthcare system billions of dollars annually. Current solutions, such as antiseptics and antibiotics, can be toxic to cells or contribute to the development of antibiotic-resistant strains of bacteria. Exposure to germicidal ultraviolet radiation (GUV) at 254 nm has been reported as an effective method for chronic wound management. However, concerns about the health hazards from exposure to 254 nm radiation have limited its use for wound management applications. In contrast, wavelengths of ultraviolet radiation in the range of 200-235 nm have exhibited similar germicidal ability but with a lower penetration range in tissue, potentially making those wavelengths better suited for chronic wound disinfection. In this study, a novel phototherapy device emitting principally at 234 nm was used to assess the killing efficacy against Methicillin-resistant Staphylococcus aureus (MRSA). Potential health hazards from exposure using the device were evaluated using a 3D human skin model. The ultraviolet exposure device tested in this study shows promise for effective decontamination of chronic nonhealing wounds without associated health hazards.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Targeting pain and inflammation: A comparative study of photobiomodulation with 532 and 660 nm lasers in rats.","authors":"Andréa Ribeiro Mattoso-Câmara, Juliana Zampoli Boava Papini, Marcos Aurélio Teixeira, Denise Nami Fujii, Giovana Radomille Tofoli, Aguinaldo Silva Garcez","doi":"10.1111/php.70013","DOIUrl":"https://doi.org/10.1111/php.70013","url":null,"abstract":"<p><p>This study evaluated the analgesic and anti-inflammatory effects of photobiomodulation (PBM) using 532 nm (green) and 660 nm (red) low-power lasers in an animal model of acute postoperative pain. Forty-five Wistar rats underwent a 1 cm surgical incision on the right hind paw and were randomly assigned to three groups: red laser (RL, 660 nm, 100 mW, 5 J, 167 J/cm<sup>2</sup>), green laser (GL, 532 nm, 70 mW, 4.97 J, 166 J/cm<sup>2</sup>), and control (LO, no irradiation). PBM was applied immediately and at 1-, 3-, 6-, and 24-h postsurgery, and pain levels were assessed using von Frey's electronic analgesimeter. Inflammatory cytokines (TNF-α, IL-1β, CGRP, and Substance P) were measured by ELISA. Results showed that both RL and GL were significantly more effective than the control group in reducing pain and inflammation. RL provided superior analgesia, increasing pain tolerance to 690.54 ± 50.20 g at 24 h, reaching levels comparable to the non-incised paw (p < 0.001). GL demonstrated greater anti-inflammatory effects, significantly reducing TNF-α levels at 1 h (p < 0.05) and 24 h and maintaining lower IL-1β and CGRP levels. RL also modulated Substance P levels, correlating with its stronger analgesic effect. These findings suggest that RL is preferable for direct pain relief, while GL is more effective in modulating inflammatory responses. Given the statistically significant improvement in pain control and inflammatory marker modulation, PBM using these wavelengths could be a valuable adjunct therapy for postoperative pain management and enhanced healing in surgical patients. Future studies should explore synergistic PBM protocols combining both wavelengths to optimize clinical outcomes.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bianca Menchicchi, Andre C Stiel, Mattia Nieddu, J P Fuenzalida-Werner
{"title":"Fluorescent proteins: A journey from the cell to extreme environments in material science.","authors":"Bianca Menchicchi, Andre C Stiel, Mattia Nieddu, J P Fuenzalida-Werner","doi":"10.1111/php.70011","DOIUrl":"https://doi.org/10.1111/php.70011","url":null,"abstract":"<p><p>This review presents the progression from the use of fluorescent proteins (FPs) and chromoproteins as bioimaging labels and sensors to the strategic engineering of their properties for robust functionality in synthetic and non-biological environments. Specifically, engineered variants of the small ultra-red fluorescent protein (smURFP) were developed and optimized for optoacoustic imaging through structure-guided mutagenesis. Reversibly switchable genetically encoded indicators were also created to enhance bioimaging capabilities. To extend the applicability of such proteins to material science and enable their function in everyday applications-such as environmental sensors, encoders, or color components in textiles and electronics-their inherent stability limitations were addressed. For this purpose, supramolecular stabilization strategies, including genetically encoded macro-oligomerization techniques, were explored. These methods effectively enhanced the resilience of FPs under chemically challenging conditions, without compromising their photophysical properties. Finally, the exploration of circularly polarized luminescence (CPL) from FPs is discussed, and their potential as CPL emitters suitable for sustainable photonic applications is identified. Overall, the transformative potential of engineered FPs as essential components for applications beyond bioimaging is emphasized.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nadezhda I Schastnaya, Tatyana E Kuznetsova, Hanna E Pyzh, Aliaksandr V Mikulich, Antonina I Tretyakova, Tatsiana S Ananich, Ludmila G Plavskaya, Vitaly Yu Plavskii
{"title":"Enhancement of pharmacotherapeutic activity of antibiotic \"Amphotericin B\" due to its photosensitizing properties.","authors":"Nadezhda I Schastnaya, Tatyana E Kuznetsova, Hanna E Pyzh, Aliaksandr V Mikulich, Antonina I Tretyakova, Tatsiana S Ananich, Ludmila G Plavskaya, Vitaly Yu Plavskii","doi":"10.1111/php.70014","DOIUrl":"https://doi.org/10.1111/php.70014","url":null,"abstract":"<p><p>The problem of increasing the efficacy of fungal infection treatment is a pressing issue in modern medicine due to increasing resistance of pathogenic microorganisms to antifungal drugs. At the same time, the development of new antifungal drugs is a complex, lengthy, and expensive task, since there is a close evolutionary relationship between fungal eukaryotes and human somatic cells. The aim of this work is to enhance the pharmacotherapeutic activity of the widely used antifungal polyene antibiotic \"Amphotericin B\". As a result of in vitro studies using somatic cells, the ability of Amphotericin B to act as a photosensitizer when exposed to light in the blue spectral region, corresponding to the absorption band of the drug, as well as to sensitize the Type II photochemical reactions (generation of singlet oxygen) and radical processes (Type I reactions) was demonstrated. When modeling contact dermatitis on depilated areas of rat skin, it was found that the combined use of Amphotericin B and radiation from super-bright LEDs with a wavelength of λ<sub>max</sub> = 405 nm enhances the fungicidal effect of the drug and also causes a reliable decrease in the characteristic signs of dermatitis compared to the effect of each of the specified factors separately. The results obtained can find wide application in medical practice for the treatment of fungal lesions of the skin, oral cavity, female genital area, and so forth. The presence of photosensitizer (Amphotericin B), approved for use, and phototherapeutic equipment corresponding to its absorption spectrum make it possible to develop the necessary medical technologies.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tanya Jaiswal, Durdana Muntaqua, Gagan Chhabra, Nihal Ahmad
{"title":"Polo-like kinases and UV-induced skin carcinogenesis: What we know and what's next.","authors":"Tanya Jaiswal, Durdana Muntaqua, Gagan Chhabra, Nihal Ahmad","doi":"10.1111/php.70002","DOIUrl":"https://doi.org/10.1111/php.70002","url":null,"abstract":"<p><p>Prolonged and chronic exposure to UV radiation is a risk factor for multiple skin cancers. As the incidence of UV-associated skin cancers continues to rise, there is a pressing need for a deeper understanding of the underlying mechanisms driving these pathologies. Polo-like kinases (PLKs), a family of enzymes consisting of five members (PLK1-PLK5), have been implicated in various aspects of skin carcinogenesis. The inhibition of PLKs is currently being explored as a potential strategy for cancer management. While much of the research has predominantly concentrated on PLK1, recent studies are increasingly shedding light on the role of other PLK family members, given their growing importance in cancer progression. Understanding the relationship between UV-associated skin cancers and PLKs could open new avenues for more effective management of skin cancers. In this review, we discuss the critical mechanisms associated with UV and PLKs in causing skin cancers, followed by the potential role of UV in modulating PLKs in different skin cancers. We also examine the prospect of targeting PLK signaling to enhance therapies for UV-induced skin cancer and improve patient responses. So far, there is not enough literature focused on the simultaneous effects of PLKs and UV using skin cancer models, emphasizing the need for further research to completely understand the role of PLKs in UV-induced skin carcinogenesis.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manuela Buonanno, Camryn Petersen, David Welch, Raabia Hashmi, David J Brenner
{"title":"222 nm far-UVC light and skin health: Assessment of DNA damage across different skin types.","authors":"Manuela Buonanno, Camryn Petersen, David Welch, Raabia Hashmi, David J Brenner","doi":"10.1111/php.70010","DOIUrl":"https://doi.org/10.1111/php.70010","url":null,"abstract":"<p><p>Due to a limited penetration into skin and eyes combined with a broad germicidal effectiveness, far-UVC light (200-235 nm) has been proposed as an effective intervention for airborne pandemic control. Specifically, 222 nm light is not predicted to damage skin because it is primarily absorbed by the proteins in the superficial stratum corneum of the epidermis. Thus, it is hypothesized that the thickness of the stratum corneum is one of the most significant contributing factors to the risk of skin damage from exposure to far-UVC. From measurements of the stratum corneum thickness in live human skin biopsies, it was found that none of the donor demographics studied had an impact on the thickness of the stratum corneum. While multiple studies suggest that exposure to 222 nm is minimally damaging to skin, a few studies to date have investigated effects as a function of skin characteristics (e.g., individual's age and sex). In selected tissues, the induction of DNA damage following an acute exposure to 100 or 500 mJ/cm<sup>2</sup> from 222 nm light was analyzed as a function of donor demographics. The results agree with previous studies using other models of human skin and show that in human skin biopsies, 222 nm induces minor DNA damage only at high doses, especially in skin with low melanin content (phototype).</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mark P Ortenzio, Sanjay Anand, Jeffrey B Travers, Edward V Maytin, Craig A Rohan
{"title":"Immunomodulatory effects of photodynamic therapy for skin cancer: Potential strategies to improve treatment efficacy and tolerability.","authors":"Mark P Ortenzio, Sanjay Anand, Jeffrey B Travers, Edward V Maytin, Craig A Rohan","doi":"10.1111/php.70008","DOIUrl":"https://doi.org/10.1111/php.70008","url":null,"abstract":"<p><p>Skin cancer, including melanoma and nonmelanoma variants, accounts for the majority of cancer diagnoses in the United States each year and is increasingly the focus of research on treatment and prevention. Photodynamic therapy (PDT) is a well-established, nonscarring treatment for nonmelanoma cancers such as early squamous precancers (actinic keratoses, AK), squamous carcinoma in situ, and superficial basal cell carcinoma (BCC). PDT can be painful during illumination and has relatively poor efficacy for thick lesions. However, these disadvantages could be ameliorated if newly recognized PDT effects on immunity were fully understood and harnessed. In this review, we highlight basic and translational studies on effects that locally administered PDT exerts on the immune system. On the one hand, PDT stimulates an anti-tumor immune response observable as peritumoral infiltration of innate and adaptive immune cells and activation of cytotoxic T cells both locally and in circulating blood. On the other hand, PDT generates systemic immunosuppression mediated by the release of subcellular microvesicle particles (MVP); the latter may be blocked by using acid sphingomyelinase (aSMase) inhibitors. The ultimate goal must be to balance two opposing forces, that is, to limit PDT-induced immunosuppression while promoting PDT-induced regression of tumors so as to favor a long-term anti-tumor immune response.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rehana Shahin, Rajesh K Yadav, Rajesh K Verma, Shaifali Mishra, Chandani Singh, Jin-OoK Baeg
{"title":"Solar-driven photocatalytic system for CO<sub>2</sub> fixation and conversion of dopamine into indole derivative.","authors":"Rehana Shahin, Rajesh K Yadav, Rajesh K Verma, Shaifali Mishra, Chandani Singh, Jin-OoK Baeg","doi":"10.1111/php.70007","DOIUrl":"https://doi.org/10.1111/php.70007","url":null,"abstract":"<p><p>Photocatalytic CO₂ fixation into solar fuels offers a promising route for renewable energy storage by converting CO₂ into chemical bonds. Among various products, formic acid is considered the most reliable candidate for industrial applications due to its high efficiency and sustainable feasibility. Various catalysts, including metals, chalcogenides, transition metals, and carbon-based materials, have been explored for this purpose. Polymeric organic frameworks are a class of crystalline polymers with tunable structures, making them potential candidates for metal-free photocatalysts. However, their low crystallinity often limits light-harvesting efficiency and photocatalytic activity, posing a challenge for industrial applications. The primary obstacles in this field are low activity and poor selectivity of photocatalysts. In this study, we propose a soft-template induction strategy to construct a metal-free heterojunction polymeric framework based on 5,15-di-(4-aminophenyl)-10,20-diphenyl porphyrin (BP) and perylene tetra-anhydride (PT), referred to as PTBP. This polymer exhibits high crystallinity and strong solar light absorption. The PTBP framework demonstrates better performance in solar-powered molecular artificial photosynthesis, achieving significant improvements over PT. Specifically, PTBP exhibits high 1,4-NADH/NADPH regeneration efficiencies (52.51%/58.41%) compared to PT (9.11%/10.1%), a substantial NADH consumption (119.25 μmol) in exclusive solar fuel production from CO₂ within 1 h, and excellent yield (50.37%) in the photocatalytic conversion of dopamine into an indole-derivative, surpassing PT (13.93%). The current finding highlights the benchmark photocatalytic potential of the PTBP polymeric framework's capacity for photocatalysis for CO<sub>2</sub> fixation and conversion of dopamine into indole derivatives.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}