Photochemistry and Photobiology最新文献

Feasibility of antimicrobial photodynamic therapy for treatment of breast abscesses: Retrospective Monte Carlo simulation study.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-03-04 DOI: 10.1111/php.14085

Timothy M Baran, Lam Nguyen, Sophia Palumbo, Anna Weiss, Nebojsa Duric

{"title":"Feasibility of antimicrobial photodynamic therapy for treatment of breast abscesses: Retrospective Monte Carlo simulation study.","authors":"Timothy M Baran, Lam Nguyen, Sophia Palumbo, Anna Weiss, Nebojsa Duric","doi":"10.1111/php.14085","DOIUrl":"https://doi.org/10.1111/php.14085","url":null,"abstract":"Breast abscesses are a common issue that affects both breastfeeding and nonpuerperal patients. Even with image-guided drainage and antibiotics, these recur frequently due to unresolved infection. One potential adjunct treatment is photodynamic therapy (PDT), which uses photosensitive compounds to generate cytotoxic reactive oxygen species. Here, we investigate the feasibility of using antimicrobial PDT to treat breast abscesses through a retrospective Monte Carlo simulation study. This study included adult subjects that had ultrasound-guided breast abscess aspiration over a five-year period at our institution (n = 119). Pre-procedure ultrasound images were segmented and used to produce either idealized ellipsoidal volumes or extrapolated segmented volumes for simulated PDT treatment planning. We investigated the effects of abscess wall optical properties and the presence of intra-cavity scattering on our ability to achieve target fluence rates of 4 or 20 mW/cm2 in 95% of the abscess wall. Without intra-cavity scattering, eligibility at the 4 mW/cm2 fluence rate target ranged from 99.2%-100% for ellipsoidal volumes and 76.1%-80.7% for segmented volumes. With intra-cavity scattering added, eligibility at the 4 mW/cm2 target increased significantly to 93.4%-100% for segmented volumes (p < 0.0001). However, this required significantly higher optical power (p < 0.0001). PDT therefore appears to be feasible in this patient population and merits further investigation.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking acetylcholinesterase inhibitor pesticides in the environment using the rapid transient of chlorophyll fluorescence.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-17 DOI: 10.1111/php.14080

Ivana López Valiño, Gonzalo Dieguez Gaviola, Virginia Emilse Diz, Graciela Alicia González, María Gabriela Lagorio, Gabriela Beatriz Cordon

{"title":"Tracking acetylcholinesterase inhibitor pesticides in the environment using the rapid transient of chlorophyll fluorescence.","authors":"Ivana López Valiño, Gonzalo Dieguez Gaviola, Virginia Emilse Diz, Graciela Alicia González, María Gabriela Lagorio, Gabriela Beatriz Cordon","doi":"10.1111/php.14080","DOIUrl":"https://doi.org/10.1111/php.14080","url":null,"abstract":"Several home pesticides are organophosphorus compounds. These compounds inhibit the enzyme acetylcholinesterase, causing harmful effects on the health of biota. Through this research, the usefulness of Glycine max (soybean) and Cichorium intybus (chicory) plants as sentinels of organophosphorus compounds in the environment was successfully tested. Different concentrations of the insecticide chlorpyrifos were tried out. Damage to plants at the photosynthetic apparatus level was evaluated by measuring the high temporal resolution variable chlorophyll fluorescence (OJIP test). Several parameters derived from this test indicated a high level of damage in both species even at the mean dose recommended for use in the field. However, a few parameters did not consistently reflect damage in leaves. A drop in the values of the maximum fluorescence (FM), the quantum yield of electron transport flux, transport between quinones A and B (ET0/ABS) and the maximal quantum yield of PSII (TR0/ABS) could alert us about the presence of organophosphates in the environment. An increase in the dissipated energy flux per reaction center (DI0/RC) values was also observed. The species showed different sensitivities, with soybean plants being the most sensitive. The OJIP transient thus becomes a valuable rapid, non-destructive tool for biomonitoring this class of pesticides in the environment.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GOUTERMAN - A module for simulations of tetrapyrrole optical spectra.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-17 DOI: 10.1111/php.14057

Hai Du, Masahiko Taniguchi, James R Diers, Christine Kirmaier, David F Bocian, Jonathan S Lindsey, Dewey Holten

{"title":"GOUTERMAN - A module for simulations of tetrapyrrole optical spectra.","authors":"Hai Du, Masahiko Taniguchi, James R Diers, Christine Kirmaier, David F Bocian, Jonathan S Lindsey, Dewey Holten","doi":"10.1111/php.14057","DOIUrl":"https://doi.org/10.1111/php.14057","url":null,"abstract":"Tetrapyrrole macrocycles comprise the pigments of life (heme, chlorophylls, etc.) and together with synthetic analogues also find wide use in materials chemistry and the life sciences. The distinct colors of the various macrocycles are essential features. A time-honored approach for understanding the electronic spectra entails analyzing the impact of molecular composition on the relative energies of the four frontier molecular orbitals (MOs) of these 18-π annulenes. While first introduced by Prof. Martin Gouterman in 1959, use of the four-orbital model has been largely conceptual. Here, a computational module (GOUTERMAN) is introduced that takes as input the energies of the four MOs, provides user control over key parameters (configuration-interaction energies, dipole strengths, spectral widths), and outputs simulated absorption spectra. The energies of the MOs can be calculated independently (e.g., from density functional theory) or drawn from a small database that is provided. The MO energies and key parameters can be toggled and spectral changes viewed. The GOUTERMAN module is available in PhotochemCAD as a tool for both specialists and non-specialists to visualize, via hands-on experience, the connections between the structural and spectral properties of the tetrapyrrole chromophores found ubiquitously in Nature and used widely in research.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized LED phototherapy induces ROS-mediated membrane damage in Trichophyton rubrum for effective onychomycosis treatment.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-17 DOI: 10.1111/php.14079

Angze Li, Yi Ren, Zhenjian Peng, Shangfeng Liu, Muqing Liu

{"title":"Optimized LED phototherapy induces ROS-mediated membrane damage in Trichophyton rubrum for effective onychomycosis treatment.","authors":"Angze Li, Yi Ren, Zhenjian Peng, Shangfeng Liu, Muqing Liu","doi":"10.1111/php.14079","DOIUrl":"https://doi.org/10.1111/php.14079","url":null,"abstract":"Onychomycosis, predominantly induced by Trichophyton rubrum, is a pervasive nail disorder within dermatology known for its high relapse rates and suboptimal patient adherence to treatment regimens. While photomedicine has emerged as a promising therapeutic modality, efficiency reductions are common due to deck obstruction in conventional light therapy. The spectral flexibility of LEDs offers a compelling solution, allowing for deeper deck penetration while maintaining efficacy. We have developed an advanced LED system with optimized optical parameters and have elucidated the antimicrobial mechanisms underlying this technology. Our research shows that an optimal wavelength of 405 nm, an energy density of 396 J/cm2, and an average light intensity of 140 mW/cm2 demonstrate superior efficacy in treating onychomycosis. The antifungal mechanism of our pulsed LED system involves the induction of reactive oxygen species (ROS) within fungal mycelia, ultimately resulting in membrane damage. These insights highlight the potential of LED lighting systems as a novel antimicrobial strategy, offering a promising avenue for the treatment of onychomycosis.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the naphthylene linker on the J-aggregation abilities of chlorophyll-a derivatives. 萘链对叶绿素-a 衍生物 J-聚集能力的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-13 DOI: 10.1111/php.14076

Yuma Hisahara, Takeo Nakano, Hitoshi Tamiaki

引用次数: 0

Excited state relaxation mechanisms and tautomerism effects in 2,6-Diamino-8-Azapurine.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-11 DOI: 10.1111/php.14045

Leonardo M F Oliveira, Danillo Valverde, Gustavo Juliani Costa, Antonio Carlos Borin

{"title":"Excited state relaxation mechanisms and tautomerism effects in 2,6-Diamino-8-Azapurine.","authors":"Leonardo M F Oliveira, Danillo Valverde, Gustavo Juliani Costa, Antonio Carlos Borin","doi":"10.1111/php.14045","DOIUrl":"https://doi.org/10.1111/php.14045","url":null,"abstract":"The photochemistry of 9H-2,6-diamino-8-azapurine (9H-8AZADAP), a promising fluorescent probe, was investigated using the Multi-State Complete-Active-Space Second-Order Perturbation Theory (MS-CASPT2) quantum chemical method, along with the Average Solvent Electrostatic Configuration and Free Energy Gradient (ASEC-FEG) and Polarizable Continuum Model (PCM) to take into account water solvation effects. For both isolated and solvated species, the main photochemical event is initiated by the absorption of light from ground-state to the bright 1(ππ* La) state, which undergoes barrierless evolution to its minimum energy region (1(ππ* La)min) without crossing any other potential energy surface (PES). Subsequently, the excess of energy is released through fluorescence. From the 1(ππ* La)min region, two radiationless decay pathways back to the initial ground state, mediated by two distinct conical intersections between the ground and 1(ππ* La) states, are found to be unlikely due to the presence of high energy barriers in both environments. Our results also indicate that the solvation effects are more pronounced when using the ASEC-FEG method, which predicts larger structural and energy changes, especially concerning energetic barriers. Based on the free energy perturbation theory (FEP), a hypothetical thermodynamic cycle was devised, from which we infer that in an aqueous environment the N3 site is the most favorable for protonation. We also conclude that the 8H-8AZADAP tautomer is responsible for the fluorescent band observed experimentally at 410 nm and elucidates the mechanism of phototautomerism.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel microcapsule composite Spherulites Peony Superior Retinol mitigates UVB-induced skin damage in vitro and in vivo. 一种新型微胶囊复合材料 Spherulites Peony Superior Retinol 可在体外和体内减轻紫外线引起的皮肤损伤。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-05 DOI: 10.1111/php.14078

Jiejun Han, Rongyue Gong, Yuankun Liu, Tiangui Gong, Bin Wang, Laidi Zhang, Jiayue Chen

{"title":"A novel microcapsule composite Spherulites Peony Superior Retinol mitigates UVB-induced skin damage in vitro and in vivo.","authors":"Jiejun Han, Rongyue Gong, Yuankun Liu, Tiangui Gong, Bin Wang, Laidi Zhang, Jiayue Chen","doi":"10.1111/php.14078","DOIUrl":"https://doi.org/10.1111/php.14078","url":null,"abstract":"Skin serves as our outermost barrier, protecting our bodies from various environmental damages. Increasing research has revealed that UVB is a primary factor for extrinsic aging. This study explored the role of a novel microcapsule composite Spherulites Peony Superior Retinol (SPSR) on skin damage induced by UVB. SPSR exhibited a capacity to eliminate UVB-induced ROS. By measurement of cyclobutane pyrimidine dimers (CPD) and comet assay, the results implied that SPSR mitigates DNA damage from oxidative damage caused by UVB. In addition, UVB radiation typically leads to an increase in inflammatory factors within the skin. Decreased gene expressions of interleukin-1α and TNF-α have been observed in HaCaT cells. Moreover, a decreased gene expression of extracellular matrix (ECM)-related protein, including fibronectin (FN1), Col1A1, and Col3A1 caused by UVB was mitigated by SPSR. Furthermore, the clinical trials with 30 volunteers confirmed the significant relief and antiwrinkle effects of the cosmetic formulation containing 0.1% SPSR. These findings implied the promising potential of SPSR as a comprehensive solution for combating the detrimental effects of UVB exposure and maintaining skin health.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inoculation of methylotrophs mitigates heat and UV stress in mung bean (Vigna radiata L.) and enhances growth, antioxidant, and functional diversity.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-03 DOI: 10.1111/php.14075

Harshida A Gamit, Salim Manoharadas, Natarajan Amaresan

{"title":"Inoculation of methylotrophs mitigates heat and UV stress in mung bean (Vigna radiata L.) and enhances growth, antioxidant, and functional diversity.","authors":"Harshida A Gamit, Salim Manoharadas, Natarajan Amaresan","doi":"10.1111/php.14075","DOIUrl":"https://doi.org/10.1111/php.14075","url":null,"abstract":"Climate change involves the induction of heat and solar ultraviolet (UV) radiation, which profoundly affects sustainable crop production. Increasing solar UV radiation negatively impacts the photosynthetic apparatus, plant-associated organisms, and plant health. The present study aimed to comprehensively assess methylotrophic bacteria to alleviate heat and UV radiation in Vigna radiata L. under pot studies and field conditions. Under normal and UVB stress conditions, inoculation of methylotrophs significantly enhanced seed germination (72.55%-96.70% (normal) and 51.67%-65.33% (stressed)) and improved plant growth parameters, total chlorophyll (25.80-48.16 mg/g (normal) and 9.13-27.88 mg/g (stressed)), and carotenoid (569.1-1067.1 μg/g (normal) and 287.8-903.4 μg/g (stressed)) contents. A similar enhancement in antioxidant properties such as superoxide dismutase (1-5 fold), peroxidase (1-9 fold), phenylalanine ammonia lyase (1-4 fold), and proline content (1-5 fold) was observed in response to UVB radiation and heat stress under pot studies. A community-level physiological profile (CLPP) study of leaf samples revealed enhanced AWCD in methylotrophs treated plants compared to the UVB-exposed controls. Furthermore, field studies in summer conditions confirmed that inoculation with methylotrophs had a positive effect on V. radiata growth and physiology. The methylotrophs inoculation increased pod formation (25.44-32.78 and 15.56-32.00) and yield (109.81-238.63 and 71.88-216.29 q/ha) under UV cut-off sheet covered and non-covered conditions, respectively. This study demonstrated the potential of methylotrophs to mitigate heat and solar (UV) radiation in plants and provide sustainable strategies for agriculture and the environment.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel acquired resistance mechanism to 5-aminolevulinic acid-mediated photodynamic therapy with ABCG2 inhibition.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-03 DOI: 10.1111/php.14077

Sharayu Chandratre, Jordyn Olsen, Bin Chen

{"title":"A novel acquired resistance mechanism to 5-aminolevulinic acid-mediated photodynamic therapy with ABCG2 inhibition.","authors":"Sharayu Chandratre, Jordyn Olsen, Bin Chen","doi":"10.1111/php.14077","DOIUrl":"https://doi.org/10.1111/php.14077","url":null,"abstract":"We report the occurrence of acquired tumor cell resistance to 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) in combination with ABCG2 inhibition. ALA-PDT in combination with either an ABCG2 tool inhibitor Ko143 or a repurposed clinically-relevant ABCG2 inhibitor lapatinib was highly effective in eradicating the H4 human glioma cells, resulting in minimal cell survival after treatment. However, after seven rounds of repeated treatments with light dose escalation, the resultant tumor cells became resistant to the combination therapy. The resistant sublines and the parental cell line showed similar ABCG2 activities and protein levels, indicating that it was not ABCG2 that caused the resistance. They also exhibited similar responses to PpIX-PDT and mTOR inhibitor AZD2014, suggesting that alterations in PDT sensitivity and mTOR pathway had little contribution to the development of resistance phenotype. By determining the intracellular and extracellular PpIX levels, the activities and protein levels of heme biosynthesis enzymes, we found that porphobilinogen deaminase (PBGD) activity and protein level were significantly reduced in the resistant sublines, causing resistance to PDT by substantially reducing PpIX biosynthesis. A novel acquired resistance mechanism to ALA-PDT with ABCG2 inhibition has been uncovered.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A skin explant model for studying UV-induced DNA damage and repair.

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-02-03 DOI: 10.1111/php.14070

Hailey Payne, Christina Athans, Shiyong Wu, Veronica Bahamondes Lorca

{"title":"A skin explant model for studying UV-induced DNA damage and repair.","authors":"Hailey Payne, Christina Athans, Shiyong Wu, Veronica Bahamondes Lorca","doi":"10.1111/php.14070","DOIUrl":"https://doi.org/10.1111/php.14070","url":null,"abstract":"There is a growing need for a skin model that combines the natural physiology of skin while reducing reliance on mice. Natural physiology is achieved by using fresh, intact skin explants sourced from living organisms such as humans or mice. This study focused on the standardization and characterization of an in vitro mouse skin explant model for investigating solar ultraviolet (sUV)-induced skin damage. We developed a protocol to use skin explants derived from the discarded tissue of mice after euthanasia. These explants consist of intact dermal and epidermal layers suspended in cell culture medium and maintained in vitro. To assess the viability of the skin explants, we evaluated tissue morphology (via hematoxylin and eosin [H&E] staining), viability markers, and DNA damage markers. Our ex vivo model preserves the key characteristics and physiological responses of in vivo skin for short incubation periods, while minimizing the use of mice. This model enables the study of DNA damage and repair, and it has broad applications, including studies on skin photoprotection, topical treatments, drug development, and cosmetics.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0