Photochemistry and Photobiology最新文献

Exploring natural sunscreens: UVB protection and antioxidant properties in gadusol-rich fish roes extracts. 探索天然防晒霜：富含gadusol的鱼卵提取物的UVB保护和抗氧化特性。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-10-06 DOI: 10.1111/php.70042

Rocío Isla Naveira, Gabriela Hollmann, José María Monserrat, Ana Paula S Votto, Julie Medeiros da Silveira, Andressa Mai Matsumoto, Lais Zortéa, Andy Joel Taipe Huisa, Agueda E Massa, M Sandra Churio

{"title":"Exploring natural sunscreens: UVB protection and antioxidant properties in gadusol-rich fish roes extracts.","authors":"Rocío Isla Naveira, Gabriela Hollmann, José María Monserrat, Ana Paula S Votto, Julie Medeiros da Silveira, Andressa Mai Matsumoto, Lais Zortéa, Andy Joel Taipe Huisa, Agueda E Massa, M Sandra Churio","doi":"10.1111/php.70042","DOIUrl":"https://doi.org/10.1111/php.70042","url":null,"abstract":"The search for natural alternatives to synthetic sunscreens has driven interest in marine compounds with antioxidant and UV-protective properties. The present study expands our understanding of the potential of gadusol by evaluating the photochemoprotective and antioxidant effects of extracts from an underexploited marine by-product: the roes of yellowtail amberjack fish (Seriola lalandi). UVB-mediated responses were studied in vitro and in vivo using HaCaT keratinocytes and Caenorhabditis elegans worms, respectively. Additionally, several antioxidant assays were conducted to evaluate the antioxidant capacity and thermal stability over time. We also tested the docking binding of gadusol to the Nrf2-binding domain of Keap1 to better understand its potential chemoprotective role. Overall, the gadusol-containing extracts exhibited remarkable stability over time, offering effective protection against UVB radiation in both in vitro and in vivo models. This information contributes to a better characterization of the functional role of gadusol in crude extracts and its relevance for the design of innovative applications in various pharmaceutical and cosmetic industries.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Far-infrared irradiation attenuates platelet-derived growth factor-stimulated vascular smooth muscle cell migration through protein phosphatase 2A-mediated Akt inhibition. 远红外照射通过蛋白磷酸酶2a介导的Akt抑制，减弱血小板源性生长因子刺激的血管平滑肌细胞迁移。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-10-02 DOI: 10.1111/php.70039

Na-Young Lee, Seo-Hyeon Kim, Young Jin Kang, Il-Kug Kim, Yun-Jin Hwang, Du-Hyong Cho

{"title":"Far-infrared irradiation attenuates platelet-derived growth factor-stimulated vascular smooth muscle cell migration through protein phosphatase 2A-mediated Akt inhibition.","authors":"Na-Young Lee, Seo-Hyeon Kim, Young Jin Kang, Il-Kug Kim, Yun-Jin Hwang, Du-Hyong Cho","doi":"10.1111/php.70039","DOIUrl":"https://doi.org/10.1111/php.70039","url":null,"abstract":"Vascular smooth muscle cell (VSMC) migration is a crucial factor contributing to the development and progression of atherosclerosis and in-stent restenosis. Although far-infrared (FIR) rays have been suggested to alleviate atherosclerosis, their effects on VSMC migration remain unclear. To investigate the underlying mechanisms, we performed wound healing migration assays, cell viability assays, transfection with wild-type (WT)-Akt gene, western blot analyses, and ex vivo studies using isolated rat aortas. FIR irradiation inhibited basal and platelet-derived growth factor (PDGF)-stimulated VSMC migration without affecting cell viability, and decreased the phosphorylation of Akt at Ser473 (p-Akt-Ser473) in a time-dependent manner. Transfection with WT-Akt gene restored FIR-suppressed VSMC migration. Co-treatment with okadaic acid, an inhibitor of protein phosphatase 2A (PP2A), reversed the FIR irradiation reduction in p-Akt-Ser473. FIR irradiation for 30 min enhanced the physical interaction between Akt and PP2Ac. A series of effects was not observed with the hyperthermal stimulus (39°C). Lastly, ex vivo sprouting assays showed that FIR irradiation markedly reduced VSMC sprouting. Furthermore, FIR irradiation decreased p-Akt-Ser473 levels in PDGF-stimulated rat aortas. These results indicate that FIR irradiation inhibits PDGF-induced VSMC migration through PP2A-dependent suppression of p-Akt-Ser473, suggesting its potential use in treating arterial occlusive disorders such as atherosclerosis and in-stent restenosis.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creation of nature-inspired PVDF-HFP@SGCN film catalysts for selective solar formic acid production from CO₂ under solar light. 受自然启发的PVDF-HFP@SGCN薄膜催化剂的创造，用于在太阳光下从二氧化碳中选择性地生产太阳甲酸。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-17 DOI: 10.1111/php.70033

Pooja Singh, Sandeep Kumar, Rehana Shahin, Satyam Singh, Shaifali Mishra, Atresh Singh, Alok Kumar Singh, Dilip Kumar Dwivedi, Navneet Kumar Gupta, Dushyant Singh Raghuvanshi, Rajesh K Yadav, Jin OoK Baeg, Ravindra Vikram Singh

{"title":"Creation of nature-inspired PVDF-HFP@SGCN film catalysts for selective solar formic acid production from CO2 under solar light.","authors":"Pooja Singh, Sandeep Kumar, Rehana Shahin, Satyam Singh, Shaifali Mishra, Atresh Singh, Alok Kumar Singh, Dilip Kumar Dwivedi, Navneet Kumar Gupta, Dushyant Singh Raghuvanshi, Rajesh K Yadav, Jin OoK Baeg, Ravindra Vikram Singh","doi":"10.1111/php.70033","DOIUrl":"https://doi.org/10.1111/php.70033","url":null,"abstract":"Inspired by natural photosynthesis, the study article offers a novel solution to the problem of creating cost-effective and efficient photocatalysts for CO2 fixation. The subject of study is a sulfur-doped graphitic carbon nitride (SGCN) photocatalyst that is metal-free and has drawn a lot of interest because of its possible uses in energy storage, biomaterials, and photocatalysis. The work presents PVDF-HFP@SGCN film, a novel photocatalyst that is created by grafting poly (vinylidene fluoride-co-hexafluoropropylene) polymer brushes onto SGCN under the influence of visible light. The remarkable properties of film photocatalysts, including their capacity to effectively harvest solar light, their compatibility with the optical bandgap, and their well-structured π-electron channels, which enable efficient charge movement, are leveraged in this method. As a result, the recently developed film photocatalyst shows exceptional effectiveness in fixing CO2, resulting in the generation of formic acid as a solar chemical with green energy. This result emphasizes how crucial the study is to developing renewable energy sources and lowering carbon emissions. Ultimately, the work makes a substantial contribution to the development of photocatalytic materials and emphasizes the possibility of PVDF-HFP@SGCN as a very efficient and adaptable catalyst for solar-driven chemical reactions, especially those involving the fixation of CO2 and the generation of renewable energy.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototherapies used for treating peri-implant diseases (PIDs). 用于治疗种植体周围疾病（pid）的光疗法。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-15 DOI: 10.1111/php.70037

Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli

{"title":"Phototherapies used for treating peri-implant diseases (PIDs).","authors":"Caroline Coradi Tonon, Alessandra Nara de Souza Rastelli","doi":"10.1111/php.70037","DOIUrl":"https://doi.org/10.1111/php.70037","url":null,"abstract":"This paper is a highlight of the paper by Shahbazi et al. in this issue of Photochemistry and Photobiology. In that systematic review with meta-analysis study, the authors selected a total of 47 papers for the systematic review, and from those, 14 papers were eligible for the meta-analysis. The papers included were based on the adjunctive treatment of peri-implant diseases (PIDs) by the use of photodynamic therapy (PDT), photobiomodulation (LLLT), hyperlight, and high-power lasers in combination with mechanical debridement (MD) used as routine treatment. The clinical findings promoted by the adjunctive treatment based on the phototherapies over the peri-implant diseases were investigated and demonstrated through the literature retrieved and discussed. Photodynamic therapy, photobiomodulation, hyperlight, and high-power lasers used as adjunctive treatments to the mechanical debridement rendered significant impact on different clinical outcomes for both mucositis and peri-implantitis diseases. Photodynamic therapy was the most common phototherapy used in the different studies, and it was capable of contributing to the improvement of important clinical parameters such as: BL (Bone Loss), BoP (Bleeding on Probing), CAL (Clinical Attachment Loss), and PD (Probing Depth); however, these improvements seem to be restricted to short-term evaluation. Yet, after long-term follow-ups, the combined use of phototherapies seems to be similar to those from the conventional treatment, especially MD alone. Among the adjunctive phototherapies discussed by Shahbazi et al., PDT and LLLT seem to be more realistic to be used in the daily clinical setting, especially because they can be more accessible to the clinicians.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic inactivation increases cell death rate on persistent Staphylococcus aureus. 光动力失活增加持久性金黄色葡萄球菌的细胞死亡率。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-15 DOI: 10.1111/php.70036

Maria Vitória Silva Pereira, Bruna Carolina Corrêa, Vanderlei Salvador Bagnato, Kate Cristina Blanco

{"title":"Photodynamic inactivation increases cell death rate on persistent Staphylococcus aureus.","authors":"Maria Vitória Silva Pereira, Bruna Carolina Corrêa, Vanderlei Salvador Bagnato, Kate Cristina Blanco","doi":"10.1111/php.70036","DOIUrl":"https://doi.org/10.1111/php.70036","url":null,"abstract":"Bacterial persistence is characterized by a subpopulation of metabolically dormant cells that exhibit transient tolerance to antibiotics, contributing to chronic and recurrent infections, particularly in Staphylococcus aureus, a pathogen responsible for severe infections. This phenomenon is evidenced by a biphasic killing curve, where an initial rapid decline is followed by a slowed death phase. Photodynamic inactivation (PDI) represents a promising strategy for microbial eradication through the generation of reactive oxygen species (ROS). This study investigated persistence formation in two S. aureus strains and evaluated the effects of PDI using curcumin. Time-kill assays with oxacillin revealed biphasic killing curves, indicative of persistence. Heritability testing confirmed that persistence was not passed on to progeny, supporting its phenotypic nature. PDI was performed using curcumin and blue light (450 nm), resulting in a dose-dependent reduction in bacterial viability. However, populations that survived PDI exhibited tolerance-like behavior, with unchanged MIC values, suggesting that ROS generated during PDI may induce a transient dormant state. Notably, post-PDI time-kill assays conducted after metabolic recovery showed a higher rate of bacterial death, indicating enhanced antibiotic susceptibility. In contrast, methicillin-resistant strains (MRSA) showed limited persistence induction, likely due to enhanced oxidative stress defenses. These are important to the understanding of bacterial physiological states when designing therapeutic strategies. The timing of antibiotic administration relative to PDI treatment plays a crucial role in treatment efficacy, which may be either enhanced or compromised depending on bacterial adaptation and recovery dynamics.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melanin in art: Photobleaching of melanin-on-paper is accompanied by robust modification of eumelanin structure. 艺术中的黑色素：纸上黑色素的光漂白伴随着真黑色素结构的强大修饰。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-12 DOI: 10.1111/php.70035

Lucy Kim, Guillermo Gosset, Nina G Jablonski, Linda Oyesiku, Kazumasa Wakamatsu, Shosuke Ito

{"title":"Melanin in art: Photobleaching of melanin-on-paper is accompanied by robust modification of eumelanin structure.","authors":"Lucy Kim, Guillermo Gosset, Nina G Jablonski, Linda Oyesiku, Kazumasa Wakamatsu, Shosuke Ito","doi":"10.1111/php.70035","DOIUrl":"https://doi.org/10.1111/php.70035","url":null,"abstract":"Melanin pigments find a diverse field of practical application including art. One of the authors has developed a method of printing images with genetically modified Escherichia coli cells that produce melanin onto paper using the screen-printing process. The method prints the cell culture and then incubates the paper over 3 days, allowing the cells to deposit the melanin as they reproduce. However, using melanin comes with challenges related to color fading of \"melanin-on-paper.\" The aim of this study was to evaluate how melanin-on-paper was photobleached. First, we characterized E. coli (EC)-melanins by spectrophotometric and chemical degradation methods, which indicated that EC-melanins consisted of 98% eumelanin and 2% pheomelanin with high purity (ca. 80%). Second, we evaluated photomodification of melanin-on-paper during 19 months of exposure to ambient light, showing that the exposure caused photobleaching of the melanin color intensity to a half with cross-linking of 5,6-dihydroxyindole-2-carboxylic acid units of eumelanin structure and photodegradation of 5,6-dihydroxyindole units. These results indicate that melanin-on-paper undergoes gradual bleaching during months of exposure to sunlight with robust changes in melanin structure. To mitigate deterioration of melanin-on-paper by sunlight, one option would be to frame it under UV protective acrylic.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photobiomodulation and platelet-rich fibrin in the gastrocnemius muscle submitted to calcaneal tendinopathy in rats. 大鼠跟骨肌腱病变对腓肠肌光生物调节和富血小板纤维蛋白的影响。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-10 DOI: 10.1111/php.70034

Pâmela Andressa Pauletto, Caroline Hammerschmitt Eduardo Schmitt, Fransael Franklyn Araújo da Silva, Maria Eduarda Luckner, Lucinéia de Fátima Chasko Ribeiro, Márcia Miranda Torrejais, Gladson Ricardo Flor Bertolini

{"title":"Photobiomodulation and platelet-rich fibrin in the gastrocnemius muscle submitted to calcaneal tendinopathy in rats.","authors":"Pâmela Andressa Pauletto, Caroline Hammerschmitt Eduardo Schmitt, Fransael Franklyn Araújo da Silva, Maria Eduarda Luckner, Lucinéia de Fátima Chasko Ribeiro, Márcia Miranda Torrejais, Gladson Ricardo Flor Bertolini","doi":"10.1111/php.70034","DOIUrl":"https://doi.org/10.1111/php.70034","url":null,"abstract":"The study investigated the impact of different treatments on recovery from calcaneal tendinopathy in rats, focusing on the gastrocnemius muscle. Tendinopathy is caused by repetitive overload, leading to structural collagen damage and chronic muscle inflammation. Three therapeutic approaches were compared: photobiomodulation (PBM), advanced platelet-rich fibrin (A-PRF) injection, and a combination of the two. Seventy-five rats were separated into five groups: control (CON), injury (LES), PRF (A-PRF), photobiomodulation (PBM) and combined therapy (A-PRF + PBM). Tendinopathy was induced by compression of the calcaneal tendon. The treatment was applied at specific intervals, and the animals were assessed for muscle strength and subjected to histological and morphometric analysis of the muscle. LES showed the lowest muscle strength. The treated groups (PBM, A-PRF and A-PRF + PBM) had an increase in strength between days 7 and 21, but there were no signs of muscle damage or significant recovery in the muscle fibers. The combined therapy group showed greater production of type III collagen in the connective tissue, indicating a more significant repair effect. In contrast, the neuromuscular junctions in the groups treated with PRF were smaller, suggesting possible structural alterations. The combination of therapies showed superior results to treatment alone, promoting greater tissue repair in the gastrocnemius muscle, especially due to the increase in type III collagen.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic treatment in glioma: Metabolic and structural evaluation after therapy. 胶质瘤的光动力治疗：治疗后的代谢和结构评价。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-07 DOI: 10.1111/php.70032

Marina Gabriela Teixeira, Luciana Maria Cortez Marcolino, Juliana Guerra Pinto, Rainara Moreno Sanches de Almeida, Isabelle Ferreira, Juliana Ferreira-Strixino

{"title":"Photodynamic treatment in glioma: Metabolic and structural evaluation after therapy.","authors":"Marina Gabriela Teixeira, Luciana Maria Cortez Marcolino, Juliana Guerra Pinto, Rainara Moreno Sanches de Almeida, Isabelle Ferreira, Juliana Ferreira-Strixino","doi":"10.1111/php.70032","DOIUrl":"https://doi.org/10.1111/php.70032","url":null,"abstract":"Gliomas are malignant tumors of the central nervous system, and one severe variant is called gliosarcoma. Photodynamic therapy (PDT) is a technique that stands out in the oncology area for minimizing side effects for the patient, triggering cell death at the site of irradiation, and can be used concomitantly with conventional treatments. This study aimed to evaluate the interaction of chlorine e6 with the cytoskeleton and mitochondria, as well as morphological changes and the death mechanism triggered after PDT. Chlorin e6 was used at concentrations of 200, 12.5, and 6.25 μg/mL, and cytoskeletal changes were analyzed by alpha-tubulin staining and mitochondrial membrane potential (MMP) analysis by JC-1 and Rhodamine 123 in flow cytometry. Surface features were examined using scanning electron microscopy, and the type of cell death mechanism was determined by flow cytometry with annexin and propidium iodide. Changes in the cytoskeleton were observed after PDT. Cytometry showed that cell death occurred predominantly via the apoptosis pathway, followed by the necrosis pathway. Chlorin e6 associated with PDT causes damage to gliosarcoma cells, regardless of concentration, showing cytoskeletal disruption, a decrease in MMP, and the percentage of cell death varies according to the concentration of PS.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145016051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alloxazine derivatives as multifunctional agents for photodynamic therapy, cancer cell imaging, and cell proliferation inhibition. Alloxazine衍生物作为光动力治疗、癌细胞成像和细胞增殖抑制的多功能药物。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-04 DOI: 10.1111/php.70027

Rubej R Khan, Sourav Kanti Seth, Reshma Mathew, Nitza V Falcón-Cruz, Chris Acquah, Steffen Jockusch, Liraz Levi, Carlos E Crespo-Hernández

{"title":"Alloxazine derivatives as multifunctional agents for photodynamic therapy, cancer cell imaging, and cell proliferation inhibition.","authors":"Rubej R Khan, Sourav Kanti Seth, Reshma Mathew, Nitza V Falcón-Cruz, Chris Acquah, Steffen Jockusch, Liraz Levi, Carlos E Crespo-Hernández","doi":"10.1111/php.70027","DOIUrl":"https://doi.org/10.1111/php.70027","url":null,"abstract":"The development of biocompatible organic photosensitizers remains an important challenge for advancing image-guided photodynamic therapy. Specifically, photosensitizers that combine strong photodynamic activity, fluorescence emission for bioimaging, decrease or stop the proliferation of cancer cells, and allow synthetic accessibility are in high demand. Herein, we report the synthesis and characterization of a new class of alloxazine-based photosensitizers (ANOMe, A8OMe and A7OMe). They are engineered through sugar conjugation and structurally modified at the C7 and C8 positions with electron-donating methoxy groups to tune their photochemistry and photobiology. These photosensitizers exhibit efficient population of long-lived triplet states, near unity singlet oxygen quantum yields, and fluorescence, as revealed by steady-state spectroscopy, time-correlated single-photon counting, and nanosecond transient absorption spectroscopy. Computational studies (DFT and TD-DFT) are combined with experimental data to disclose their electronic relaxation mechanisms. In vitro cellular assays demonstrate that these photosensitizers enter the cytoplasm, generate cytotoxic reactive oxygen species upon light activation, exhibit substantial fluorescence, and can significantly slow down the proliferation of cancer cells in the absence of light. Collectively, the experimental and computational results demonstrate the utility of rationally designed alloxazine derivatives as multifunctional agents for image-guided photodynamic therapy.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of 222-nm ultraviolet C irradiation bactericidal effect on the surgical field in a rabbit model. 222nm紫外线C照射对兔手术野杀菌作用的研究。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-09-02 DOI: 10.1111/php.70031

Tomoaki Fukui, Yuya Yamamoto, Kyohei Takase, Keisuke Oe, Kenichi Sawauchi, Ryota Nishida, Takahiro Niikura, Rena Kaigome, Masahiro Sasaki, Toru Koi, Hiroyuki Ohashi, Ryosuke Kuroda

{"title":"Investigation of 222-nm ultraviolet C irradiation bactericidal effect on the surgical field in a rabbit model.","authors":"Tomoaki Fukui, Yuya Yamamoto, Kyohei Takase, Keisuke Oe, Kenichi Sawauchi, Ryota Nishida, Takahiro Niikura, Rena Kaigome, Masahiro Sasaki, Toru Koi, Hiroyuki Ohashi, Ryosuke Kuroda","doi":"10.1111/php.70031","DOIUrl":"https://doi.org/10.1111/php.70031","url":null,"abstract":"Ultraviolet C (UV-C) not only has a bactericidal effect, but is also cytotoxic; however, UV-C at a wavelength of 222 nm with a high absorption coefficient for proteins is considered safe. We have previously reported the safety of 222-nm UV-C irradiation in humans and rabbits. This study evaluated the bactericidal effect of 222-nm UV-C irradiation on exposed surgical fields. Sixteen-week-old female rabbits were used, and the exposed area on their backs was sprayed with a bacterial solution from swabs collected from their soles. Three groups were formed based on UV-C irradiation: 500 mJ/cm2 of 222-nm UV-C, 200 mJ/cm2 of 254-nm UV-C, which is commonly used in germicidal lamps, and non-UV-C irradiation. The bacterial colonies were counted after irradiation. Both UV-C groups showed a significant reduction in bacterial colonies compared to the nonirradiated group, with no significant difference between the two UV-C groups. Microbiota analysis identified species that could cause surgical site infections. The results of the study suggest that 500 mJ/cm2 of 222-nm UV-C irradiation effectively reduces bacterial load, with a bactericidal effect comparable to 254-nm UV-C; hence, 222-nm UV-C irradiation is a promising and safe tool for minimizing the risk of surgical site infections.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0