Photochemistry and Photobiology最新文献

{"title":"Susceptibility of bacterial species commonly found in abdominal abscesses to low-dose photodynamic therapy: Effects of methylene blue concentration, fluence rate, and fluence.","authors":"Darrian S Hawryluk, Martin S Pavelka, Timothy M Baran","doi":"10.1111/php.14092","DOIUrl":"https://doi.org/10.1111/php.14092","url":null,"abstract":"<p><p>The objective of this study was to determine the effects of methylene blue (MB) concentration, laser fluence rate, and laser fluence on the efficacy of in vitro photodynamic therapy (PDT) for four bacteria commonly found in human abscesses. PDT experiments were performed with four of the most common bacteria found in abdominal abscesses: Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Pseudomonas aeruginosa. MB concentration was varied from 50 to 300 μg/mL, and the laser fluence rate was varied from 1 to 4 mW/cm² at a fluence of 7.2 J/cm². Higher fluence rates and fluences were explored for P. aeruginosa. Primary outcomes were the reduction in colony-forming units (CFU) following PDT and measured MB uptake following drug incubation. Gram-positive bacteria (E. faecalis and S. aureus) were eradicated at all MB concentrations and laser fluence rates tested. Efficacy was reduced for E. coli but still resulted in >6 log₁₀ reduction in CFU when MB concentration was at least 100 μg/mL. P. aeruginosa required higher fluence (28.8 J/cm²) to achieve comparable efficacy, while increasing fluence rate did not have a significant effect on PDT efficacy. MB uptake was reduced in Gram-negative species compared to Gram-positive species, particularly P. aeruginosa, although uptake was not significantly correlated with CFU reduction. Gram-positive bacteria can be eradicated in vitro with low levels of MB (50 μg/mL), laser fluence (7.2 J/cm²), and laser fluence rate (1 mW/cm²). E. coli showed substantial cell killing (>6 log₁₀ CFU reduction) with these same parameters. Low MB uptake and PDT efficacy in P. aeruginosa could be overcome by increasing the laser fluence, while increasing fluence rate did not have an effect.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term sub-erythemal UVB exposure does not impact circadian rhythms in mice under standard and rotating shift light conditions.","authors":"Adam J Greer, Sean J Cone, Rachel Bernstein, Elizabeth Schuetz, Narayani Prasad Kar, Bala S C Koritala, Shobhan Gaddameedhi","doi":"10.1111/php.14081","DOIUrl":"https://doi.org/10.1111/php.14081","url":null,"abstract":"<p><p>The International Agency for Research on Cancer (IARC) stated that circadian disruption is a potential carcinogen. However, the impact of environmental carcinogens, including sub-erythemal doses of UVB exposure, on circadian rhythms remains unclear. We evaluated the impact of long-term rotating shift, loss of Per1/2 genes, and chronic UVB exposure on the circadian rhythms of SKH-1 mice for up to 7 months. Real-time locomotion and circadian gene expression were measured in these animals. Mice under rotating shift exhibited a longer period of activity of up to 25.20 h, while those under standard light conditions had a clear 24-h rhythm. mPer1/mPer2 mice, conversely, displayed a shortened period of activity of 23.61 h. Interestingly, chronic UVB exposure had no impact on activity rhythms, though it induced skin tumors in all mice. Rotating shift and loss of mPer1/mPer2 led to circadian dysregulation of all core clock genes, with a notable phase difference in Cry1. These findings provide novel insights into environmental and genetic influences on circadian rhythms.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decomposition of water over Picene-derivatized photocatalysts under visible light irradiation.","authors":"Hayato Shiroma, Yusuke Motoyama, Hiroshi Danjo, Yuichi Ichihashi","doi":"10.1111/php.14094","DOIUrl":"https://doi.org/10.1111/php.14094","url":null,"abstract":"<p><p>Inorganic semiconductors such as TiO₂ absorb ultraviolet light to promote reactions. However, sunlight consists mainly of visible light, and so the development of photocatalysts that can use visible light is being actively researched. Density functional theory (DFT) calculations previously showed that the introduction of formyl, carboxyl, and carboxyethyl groups into picene can absorb visible light and generate hydrogen via the decomposition of water. In this study, these substituted picene derivatives were employed as photocatalysts, and their water photolysis activities were evaluated under visible light irradiation. All three picene derivatives were active photocatalysts, with hydrogen production activities decreasing in the order of: picene-13-carbaldehyde (CHO-picene) > picene-13-carboxylic acid (COOH-picene) ≈ Ethyl picene-13-carboxylate (COOEt-picene) > picene. Subsequently, the effects of the three functional groups on the hydrogen production activity in the water photolysis reaction were investigated in further detail. The effects of the functional groups were found to be in accordance with the Hammett equation, wherein a greater electron-withdrawing property led to a higher hydrogen production activity.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversal of allodynia and enhanced tendon repair in rats via 660 and 808 nm photobiomodulation.","authors":"Elia Francesco Vedda, Heloísa Alonso Matielo, Mariana Corrêa Sousa, Victhor Teixeira de Oliveira, Victória Regina da Silva Oliveira, Adilson Silva Alves, Luiz Roberto Giorgetti Britto, Camila Squarzoni Dale","doi":"10.1111/php.14082","DOIUrl":"https://doi.org/10.1111/php.14082","url":null,"abstract":"<p><p>Our study aimed to explore the potential of Photobiomodulation (PBM) as a treatment for Tendinopathy (TP), a chronic condition characterized by tendon degeneration, inflammation, and pain, for which current treatment options often yield unsatisfactory outcomes. PBM, utilizing low-level laser, has shown promise in reducing inflammatory markers, stimulating cell proliferation, and alleviating pain, thus presenting itself as a promising alternative therapy for TP. Using an experimental rat model of tendinopathy, male Wistar rats weighing 250-370 g were induced with TP through the injection of type I collagenase into the calcaneal tendon. Animals were divided into four equal groups (control, TP, TP + 660 nm, TP + 808 nm), with 10 animals in each group for the nociceptive behavioral tests and 5 in each for the biomolecular analysis. Fifteen days later, PBM was administered to the tendons once daily for seven consecutive sessions using two wavelengths (660 and 808 nm), with 3 J for 30 s, irradiating an area of 0.028 cm² totaling 107.14 J/cm². Mechanical nociception and thermal cold sensitivity were assessed, and tendon samples were collected for analysis of collagen types and total cell count. Spinal cord samples were also collected and immunostained to evaluate specific markers. Our results demonstrated that collagenase injection induced mechanical allodynia and cold hypersensitivity, both of which were reversed by PBM at both 660 and 808 nm wavelengths (**p < 0.005 and *p < 0.05). Additionally, TP led to an increase in collagen type III and a decrease in collagen type I expression, with these effects being reversed by PBM treatment (*p < 0.05). Moreover, TP increased astrocyte immunoreactivity, while PBM at both wavelengths led to a decrease in astrocyte and IL-1β expression. In conclusion, PBM emerged as an effective treatment for TP, as it not only alleviated pain symptoms but also modulated collagen expression and reduced astrocyte and IL-1β expression (**p < 0.01). These findings suggest that PBM could serve as a beneficial adjunctive therapy for analgesia and regeneration in TP management.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of the fluorescence of graphene oxide micro-flakes with addition of peripheral benzimidazole group.","authors":"Henrique Luiz Berthault Moura, Marcelo Henrique Gehlen","doi":"10.1111/php.14087","DOIUrl":"https://doi.org/10.1111/php.14087","url":null,"abstract":"<p><p>Graphene oxide (GO) is peripherally modified with the addition of 4-(1H-benzimidazol-2-yl) benzaldehyde using a Debus-Radziszewski reaction with its ortho-quinone groups. The GO content of ortho-quinone is enhanced by the oxidation of catechol groups using molybdic acid in a Froehde reaction. Following a Knoevenagel condensation of the peripherally substituted benzimidazole with methyl cyanoacetate, a fluorescent intramolecular charge-transfer (ICT) chromophore is introduced in the GO structure, forming a new fluorescent material called GO-BI (graphene oxide with benzimidazole). Its micro-flake particle has emission concentrated in its border, as revealed by confocal and total internal reflection fluorescence microscopy (TIRFM) images. The emission spectrum of the GO-BI micro-particle is centered on 525 nm and the fluorescence decay is biexponential, with two main components of 1.6 and 7.0 ns when excited at 473 nm.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of 807-nm light-emitting diode photobiomodulation on peripheral nerve injury.","authors":"Biao Chang, Qi Quan, Xinyue Liang, Yanjun Guan, Xing Xiong, Haixia Qiu, Jing Zeng, Yidi Liu, Ying Wang, Jiang Peng, Ying Gu","doi":"10.1111/php.14090","DOIUrl":"https://doi.org/10.1111/php.14090","url":null,"abstract":"<p><p>Photobiomodulation (PBM) has demonstrated potential in promoting peripheral nerve regeneration. However, there is a limited and inconclusive study on the application of light-emitting diode (LED) for nerve injury repair. In this study, we designed an 807-nm LED device with high luminous uniformity to investigate the effects of LED-based PBM on peripheral nerve injury repair. RSC96 cells were utilized as the target cells and optimal light parameters were selected based on ATP concentration, CCK-8 assays, PCR, and immunofluorescent staining. One-way analysis of variance and Student's t-test was used for statistical tests. LED irradiation at 10 mW/cm² for 30 min effectively enhanced Schwann cell proliferation, neurotrophic factor secretion, and MBP synthesis. To translate the cellular light parameters for animal studies, the transmittance through freshly obtained rat skin and biceps femoris muscle was assessed, and the transmittance was approximately 30%. After directly daily irradiating the sciatic nerve injury area in rats for 21 days at 33 mW/cm² for 30 min, we used gait analysis, immunofluorescence staining, muscle wet weight ratio, Masson staining, and transmission electron microscopy to evaluate nerve recovery. LED irradiation significantly improved sciatic nerve index, increased MBP staining intensity, and enhanced myelin sheath thickness, nerve diameter, and axon diameter. These results indicated the feasibility of LED-based PBM as a treatment method for peripheral nerve injuries.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic priming overcomes platinum resistance from short-term exposure to select perfluoroalkyl substances in endometrial cancer cell lines.","authors":"Brittany P Rickard, Lauren A Sapienza-Lundie, Marta Overchuk, Xianming Tan, Victoria L Bae-Jump, Melinda S Yates, Suzanne E Fenton, Imran Rizvi","doi":"10.1111/php.14073","DOIUrl":"https://doi.org/10.1111/php.14073","url":null,"abstract":"<p><p>First-line treatment for advanced-stage or recurrent endometrial cancer consists of platinum- and taxane-based chemotherapy, to which many patients will develop resistance. Determining the factors that contribute to platinum resistance and developing alternate treatment options for patients with advanced-stage gynecologic malignancies is critical to improving survival outcomes. Recently, we published the first study evaluating the contribution of perfluoroalkyl substances (PFAS) exposure to platinum resistance in endometrial cancer cell lines and found that select PFAS induce carboplatin resistance, potentially by dysregulating mitochondrial function. The present study expands upon those findings by examining the efficacy of photodynamic priming (PDP) in combination with carboplatin to overcome PFAS-induced platinum resistance. Due to the suspected role of mitochondrial dysfunction in platinum resistance, two clinically approved photosensitizers that, in part, localize to mitochondrial membranes or are synthesized in mitochondria were evaluated: benzoporphyrin derivative (BPD) and aminolevulinic acid-induced protoporphyrin IX (ALA-PpIX), respectively. Combination of ALA-PpIX-mediated PDP + carboplatin resulted in a greater reduction in survival fraction than the same combination with BPD. While PDP with both photosensitizers reduced mitochondrial membrane potential, the reduction was greater with BPD-PDP than ALA-PpIX-PDP. These findings demonstrate that BPD-PDP and ALA-PpIX-PDP in combination with carboplatin can be used to overcome PFAS-induced platinum resistance in endometrial cancer cells.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selectivity and anti-tumor immune elevation by vascular-targeted photodynamic therapy of mouse orthotopic bladder cancer model.","authors":"Jie Chen, Natalia Kudinova, Rebecca Dubrovsky, Jasmine Thomas, Karan Nagar, Lucas Nogueira, Avigdor Scherz, Kwanghee Kim, Jonathan Coleman","doi":"10.1111/php.14048","DOIUrl":"https://doi.org/10.1111/php.14048","url":null,"abstract":"<p><p>Vascular-targeted photodynamic therapy (VTP) with WST11 is a non-surgical tumor ablation approach that is currently being tested in a phase 3 clinical trial for the treatment of upper tract urothelial cancer. WST11-VTP utilizes illumination, leading to hypoxia, and production of free radicals followed by coagulative necrosis. Here, we tested the hypothesis that WST11-VTP can safely ablate muscle-invasive MB-49- luc bladder tumors in an orthotopic mouse model while sparing the surrounding normal tissue. For the safety study, normal mouse bladders were WST11-VTP treated. Fourteen days post-VTP granulomas in local areas around the ablation zone were noticed, which recovered after 44 days. MB49-luc orthotopic tumors at the muscle-invasive stage appeared to be effectively ablated by VTP 4-10 days post-treatment. The anti-tumor response was reflected in the increased invasion of CD4⁺, CD8⁺ T cells, myeloid CD11b⁺ cells, and NK cells in tumor tissue at 7 days post-therapy. Moreover, VTP therapy prolonged the survival of mice bearing orthotopic tumors compared with the untreated control. These results suggest that VTP can selectively ablate malignant tumors in the bladder and promote a robust anti-tumor response in a mouse model that can further augment the therapeutic outcome.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility of antimicrobial photodynamic therapy for treatment of breast abscesses: Retrospective Monte Carlo simulation study.","authors":"Timothy M Baran, Lam Nguyen, Sophia Palumbo, Anna Weiss, Nebojsa Duric","doi":"10.1111/php.14085","DOIUrl":"10.1111/php.14085","url":null,"abstract":"<p><p>Breast abscesses are a common issue that affects both breastfeeding and nonpuerperal patients. Even with image-guided drainage and antibiotics, these recur frequently due to unresolved infection. One potential adjunct treatment is photodynamic therapy (PDT), which uses photosensitive compounds to generate cytotoxic reactive oxygen species. Here, we investigate the feasibility of using antimicrobial PDT to treat breast abscesses through a retrospective Monte Carlo simulation study. This study included adult subjects that had ultrasound-guided breast abscess aspiration over a five-year period at our institution (n = 119). Pre-procedure ultrasound images were segmented and used to produce either idealized ellipsoidal volumes or extrapolated segmented volumes for simulated PDT treatment planning. We investigated the effects of abscess wall optical properties and the presence of intra-cavity scattering on our ability to achieve target fluence rates of 4 or 20 mW/cm² in 95% of the abscess wall. Without intra-cavity scattering, eligibility at the 4 mW/cm² fluence rate target ranged from 99.2%-100% for ellipsoidal volumes and 76.1%-80.7% for segmented volumes. With intra-cavity scattering added, eligibility at the 4 mW/cm² target increased significantly to 93.4%-100% for segmented volumes (p < 0.0001). However, this required significantly higher optical power (p < 0.0001). PDT therefore appears to be feasible in this patient population and merits further investigation.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative evaluation of the effects of 635 nm laser on cell proliferation and osteogenic differentiation of buccal fat pad mesenchymal stem cells.","authors":"Ardavan Etemadi, Mohammad Amin Karimi, Mohammadreza Karimi, Mahshid Hodjat, Mahsa Sheibani Pour, Alireza Karimi, Nasim Chiniforush","doi":"10.1111/php.13992","DOIUrl":"10.1111/php.13992","url":null,"abstract":"<p><p>The purpose of this study was to evaluate the effects of 635 nm diode laser with different powers on undifferentiated mesenchymal stem cells obtained from buccal fat pad. Human buccal fat stem cells were cultured in DMEM containing 10% FBS, penicillin, and streptomycin under 5% CO₂ and 95% humidity. Cells were cultured in 96-well plate and 24 h later, laser irradiation with 635 nm diode laser was performed in four groups of 200, 300, 400, and 500 mW powers in addition to the control group with the same energy density of 4 J/cm². MTT and flow cytometry assay was performed to evaluate cell proliferation and viability on 2 and 4 days after irradiation. Alizarin red assay and real-time PCR (OPN, OCN, ALP, and RUNX-2 genes) was performed to evaluate osteogenic differentiation. According to the MTT assay, none of the mentioned powers of 635 nm diode laser had significant effect on cell proliferation. Cells irradiated with power of 400 mW and 500 mW significantly showed a greater number of necrotic cells compared to the control group in Day 4. Cells irradiated with 300 mW power significantly exhibited a greater amount of nodule formation compared to all groups. Results of this study indicated that 635 nm diode laser with energy density of 4 J/cm² has a positive effect inducing osteogenic differentiation when applying with a power of 300 mW in buccal fat pad mesenchymal stem cells.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"282-289"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}