Photochemistry and Photobiology最新文献_第2页

Far-field bioluminescent immune cell-mediated photodynamic therapy is limited by photon flux. 远场生物发光免疫细胞介导的光动力治疗受到光子通量的限制。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-22 DOI: 10.1111/php.70149

Ji Tae Park, Sudip Timilsina, Rebecca C Harman, Chi-Yong Eom, Jeonghyun Oh, Qianqian Fang, Nozomi Nishimura, Chris B Schaffer, Bryan Q Spring

{"title":"Far-field bioluminescent immune cell-mediated photodynamic therapy is limited by photon flux.","authors":"Ji Tae Park, Sudip Timilsina, Rebecca C Harman, Chi-Yong Eom, Jeonghyun Oh, Qianqian Fang, Nozomi Nishimura, Chris B Schaffer, Bryan Q Spring","doi":"10.1111/php.70149","DOIUrl":"https://doi.org/10.1111/php.70149","url":null,"abstract":"Photodynamic therapy (PDT) is a clinically established photochemical treatment in which light activation of photosensitizers (PS) generates reactive species, such as reactive oxygen species (ROS), that induce localized cytotoxicity. Beyond direct tumor ablation, PDT can modulate the tumor microenvironment, enhancing antitumor immunity and sensitizing tumors to combination therapies. Related sublethal regimens, termed photodynamic priming (PDP), aim to exploit these immunomodulatory effects. While deep tumors can be accessed using fiber-optic or endoscopic light delivery, extending PDT or PDP to disseminated metastatic disease would require numerous invasive insertions, limiting clinical practicality. Here, we examine immune cell-mediated light delivery, in which luciferase-expressing immune cells infiltrate tumors and act as internal bioluminescent sources for photodynamic activation. Although this strategy could enable PDT and PDP across widespread lesions without external illumination, it remains unclear whether far-field photon emission from luciferase is sufficient to activate PS, or whether near-field mechanisms such as bioluminescence resonance energy transfer are required. Using luciferase-engineered tumor-associated macrophages and verteporfin, we observed no detectable ROS generation or tumor cell cytotoxicity in vitro. Complementary Monte Carlo simulations incorporating tissue scattering and absorption show that achievable photon fluxes are several orders of magnitude below established thresholds for cytotoxic PDT. These findings quantitatively define the limits of far-field bioluminescence and highlight the need for near-field strategies for immune cell mediated PDT and PDP.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV signature mutation hyperhotspots as genomic dosimeters. 紫外特征突变超热点作为基因组剂量计。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-22 DOI: 10.1111/php.70159

Vijay Menon, Alejandro García-Ruiz, Susan Neveu, Brenda Cartmel, Leah M Ferrucci, Meg Palmatier, Christine Ko, Kenneth Y Tsai, Mio Nakamura, Sa Rang Kim, Michael Girardi, Karl Kornacker, Douglas E Brash

{"title":"UV signature mutation hyperhotspots as genomic dosimeters.","authors":"Vijay Menon, Alejandro García-Ruiz, Susan Neveu, Brenda Cartmel, Leah M Ferrucci, Meg Palmatier, Christine Ko, Kenneth Y Tsai, Mio Nakamura, Sa Rang Kim, Michael Girardi, Karl Kornacker, Douglas E Brash","doi":"10.1111/php.70159","DOIUrl":"https://doi.org/10.1111/php.70159","url":null,"abstract":"Screening for UV-sensitive DNA mutation sites as dosimeters for individual sun exposure, using high-fidelity Duplex Sequencing to reduce false positives and a stringent genome alignment protocol to reduce false negatives, revealed: (i) Sun-exposed human skin contained UV signature mutations (C → T at a diypyrimidine) >1000-fold more frequently than unexposed neonatal skin, making these mutations sensitive genomic dosimeters for UV exposure. (ii) Tumor suppressor gene hotspot nucleotides (nt) reached variant allele frequency (VAF) 0.1%-10% via 30-3000 fold clonal expansion in occasional biopsies, due to being cancer drivers. (iii) Cyclobutane pyrimidine dimer (CPD) hyperhotspots attained those frequencies in nearly every biopsy, due to profuse creation plus ~30-fold clonal expansion. (iv) Neonatal keratinocyte cultures exposed to weeks of low-dose UVB exposure developed mutations at skin cancer tumor suppressor gene hotspot nt rarely and randomly, as expected. (v) CPD hyperhotspots in gene promoters acquired mutations in days at 1000-fold higher frequencies. Sun exposure is the strongest risk factor for skin cancer, so quantifiable UV signature mutations at CPD hyperhotspots in normal skin may make it technically feasible to identify individuals in need of monitoring to ensure early detection.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-photosensitizer polylactic acid filaments for 3D-printed photodynamic antimicrobial materials. 用于3d打印光动力抗菌材料的双光敏剂聚乳酸长丝。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-18 DOI: 10.1111/php.70156

Jesús Gómez, Michael Fleming, Nitika Paudyal, Taufiq Khan, Andrés M Durantini

{"title":"Dual-photosensitizer polylactic acid filaments for 3D-printed photodynamic antimicrobial materials.","authors":"Jesús Gómez, Michael Fleming, Nitika Paudyal, Taufiq Khan, Andrés M Durantini","doi":"10.1111/php.70156","DOIUrl":"https://doi.org/10.1111/php.70156","url":null,"abstract":"3D-printed photodynamic materials offer a route to fabricate light-activated antimicrobial objects, but their performance depends strongly on photosensitizer (PS) retention, accessibility, and photophysical behavior after thermal processing. Here, rose bengal (RB) and zinc(II) phthalocyanine (ZnPc) were incorporated into polylactic acid (PLA) at 0.5% (w/w) and processed by melt extrusion and three-dimensional printing. The characteristic absorption and fluorescence emission bands of both chromophores were retained after processing, and the dual formulation provided complementary absorption in the green and red regions of the visible spectrum. RB release from the dual-PS material followed biexponential kinetics, with kfast = 1.39 ± 0.29 min-1 and kslow = 0.013 ± 0.004 min-1. Under white-light irradiation, RB-containing materials produced approximately 5-6 log reductions (>99.999%) in bacterial viability, with Bacillus subtilis inactivated more rapidly than Escherichia coli. ZnPc-containing PLA alone produced only partial inactivation, and no appreciable dark toxicity was observed. These results identify PS accessibility as a critical parameter governing the performance of melt-processed photodynamic materials.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149002422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theoretical study on the catalytic mechanism of oxyluciferin hydrolysis by luciferin-regenerating enzyme in firefly bioluminescence substrate regeneration. 萤火虫生物发光底物再生中荧光素再生酶水解氧荧光素催化机理的理论研究。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-18 DOI: 10.1111/php.70152

Jinyu Wang, Deping Hu, Ya-Jun Liu

{"title":"Theoretical study on the catalytic mechanism of oxyluciferin hydrolysis by luciferin-regenerating enzyme in firefly bioluminescence substrate regeneration.","authors":"Jinyu Wang, Deping Hu, Ya-Jun Liu","doi":"10.1111/php.70152","DOIUrl":"https://doi.org/10.1111/php.70152","url":null,"abstract":"Firefly bioluminescence (BL) is widely utilized in bioimaging due to its high quantum yield; however, its performance in long-term monitoring is limited by rapid signal decay resulting from product inhibition by oxyluciferin (oLu). In nature, fireflies employ the luciferin-regenerating enzyme (LRE) to catalyze oLu hydrolysis, thereby removing inhibition and initiating substrate regeneration for subsequent emission. While previous studies have established the three-step regeneration pathway-LRE-catalyzed oLu hydrolysis, CHBT condensation with L-Cys, and L/D-luciferin isomerization-the initiating step lacks molecular-level clarity. Specifically, how the active site environment of LRE facilitates this reaction remains unknown, which hinders a comprehensive understanding of the recycling process and the rational optimization of sustained bioluminescent systems. To address this, we investigate its microscopic mechanism using molecular dynamics (MD) simulations and quantum mechanics/molecular mechanics (QM/MM) calculations. The results reveal that LRE provides a critical residue, Asp111, which acts as a general base to facilitate nucleophilic addition. The subsequent ring opening follows a stepwise mechanism, characterized by sequential C4-N3 bond cleavage and proton transfer-coupled C2-S1 bond breaking as the rate-determining step. Overall, this work elucidates the molecular role of LRE in substrate regeneration and provides a theoretical foundation for understanding how fireflies restart their bioluminescent cycles.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ABCG2 knockdown on therapeutic enhancement of 5-aminolevulinic acid with lapatinib. ABCG2敲除对拉帕替尼增强5-氨基乙酰丙酸治疗的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-16 DOI: 10.1111/php.70157

Jordyn Wescoat, Corinna Cabral, Bin Chen

{"title":"Effects of ABCG2 knockdown on therapeutic enhancement of 5-aminolevulinic acid with lapatinib.","authors":"Jordyn Wescoat, Corinna Cabral, Bin Chen","doi":"10.1111/php.70157","DOIUrl":"https://doi.org/10.1111/php.70157","url":null,"abstract":"5-Aminolevulinic acid (ALA) is administered as a prodrug for tumor fluorescence detection and photodynamic therapy (PDT) after being converted endogenously to the active drug protoporphyrin IX (PpIX). As a substrate of the ABCG2 transporter, PpIX is subject to outward transport in cells with ABCG2 expression, resulting in reduced PpIX fluorescence and PDT effect. Due to the lack of approved ABCG2 inhibitors, lapatinib (Lap), a kinase inhibitor approved for cancer treatment, has been repurposed as an ABCG2 inhibitor for the enhancement of ALA. To provide evidence that Lap enhances ALA by inhibiting ABCG2, we knocked down ABCG2 in four tumor cell lines and evaluated the effects of Lap on enhancing ALA-PpIX fluorescence and PDT in the wild-type and ABCG2-knockdown cell lines. ABCG2 knockdown enhanced ALA-PpIX fluorescence and PDT response, underscoring the importance of ABCG2 in regulating intracellular PpIX accumulation. However, compared with the corresponding wild-type cell lines, Lap induced less enhancement of PpIX fluorescence and PDT in ABCG2-knockdown cell lines, particularly at high concentrations and in cell lines with low ABCG2 activities. By downregulating ABCG2 in tumor cells, we showed that Lap-induced ALA enhancement was reduced, demonstrating that Lap enhances ALA by blocking ABCG2-mediated PpIX efflux.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rose Bengal-functionalized chitosan spheres as singlet oxygen generators for photodegradation of water contaminants and photodynamic inactivation. 玫瑰壳聚糖功能化球在水污染物光降解和光动力失活中的单线态氧发生器。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-11 DOI: 10.1111/php.70155

Alberto Barrera, Julieta Sacchetto, Carolina Palacios, Carolina Waiman, Eduardo Gatica, José Natera, Carolina Gambetta, Graciela Zanini, Walter A Massad

{"title":"Rose Bengal-functionalized chitosan spheres as singlet oxygen generators for photodegradation of water contaminants and photodynamic inactivation.","authors":"Alberto Barrera, Julieta Sacchetto, Carolina Palacios, Carolina Waiman, Eduardo Gatica, José Natera, Carolina Gambetta, Graciela Zanini, Walter A Massad","doi":"10.1111/php.70155","DOIUrl":"https://doi.org/10.1111/php.70155","url":null,"abstract":"Millimeter-sized chitosan spheres covalently functionalized with Rose Bengal (ME-CS-RB) were developed as reusable heterogeneous photosensitizers capable of generating singlet oxygen (1O2) under visible-light irradiation. Structural and spectroscopic characterization confirmed the effective immobilization of the photosensitizer within the chitosan matrix without compromising its integrity. The photoactivity of ME-CS-RB was evaluated through the photosensitized degradation of furosemide as a model water contaminant under green LED irradiation, with scavenger experiments indicating that 1O2 is the predominant reactive oxygen species involved, consistent with a Type II photosensitization mechanism. The spheres exhibited good operational stability over successive reuse cycles. In parallel, the photodynamic potential of the system was demonstrated through the effective inactivation of both Gram-positive (Enterococcus faecalis) and Gram-negative (Escherichia coli) bacteria under visible-light irradiation. The environmental relevance of the process was further assessed using phytotoxicity assays, which confirmed the neutralization of furosemide toxicity after photodegradation. Overall, these results demonstrate that ME-CS-RB spheres constitute a versatile photoactive platform integrating pollutant degradation and photodynamic inactivation, relevant to photochemical and photobiological applications.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of light source and fluence on the photodynamic response of RAW 264.7 macrophages containing indocyanine green-bovine serum albumin complexes. 光源及影响对含有吲哚菁绿-牛血清白蛋白复合物的RAW 264.7巨噬细胞光动力学响应的影响。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-10 DOI: 10.1111/php.70150

Magdalena Szpunar, Wiktoria Mytych, Jacek Tabarkiewicz, Zenon Czuba, Aleksandra Kawczyk-Krupka, Alina Pietryszyn-Bilińska, Andrzej Wal, David Aebisher

{"title":"The effect of light source and fluence on the photodynamic response of RAW 264.7 macrophages containing indocyanine green-bovine serum albumin complexes.","authors":"Magdalena Szpunar, Wiktoria Mytych, Jacek Tabarkiewicz, Zenon Czuba, Aleksandra Kawczyk-Krupka, Alina Pietryszyn-Bilińska, Andrzej Wal, David Aebisher","doi":"10.1111/php.70150","DOIUrl":"https://doi.org/10.1111/php.70150","url":null,"abstract":"Photodynamic therapy (PDT) efficacy depends on both the photophysical properties of the photosensitizer and the spectral characteristics of the irradiation source. In this study, the photodynamic response of RAW 264.7 macrophages incubated with an indocyanine green-bovine serum albumin (ICG-BSA) complex was evaluated using three irradiation sources: an 820 nm diode laser (emission maximum ~790 nm), a 660 nm diode laser, and a broadband tungsten-halogen OSL2 illuminator. The photophysical stability of the ICG-BSA complex was assessed by UV-Vis spectroscopy, while PDT activity was evaluated by cell viability and fluorescence measurements. The ICG-BSA complex exhibited good spectral stability and maintained characteristic absorption bands associated with monomeric and dimeric forms of ICG. Irradiation induced a fluence-dependent decrease in cell viability and fluorescence intensity, indicating effective photodynamic activation and photobleaching of the photosensitizer. The strongest photodynamic response was observed for the 820 nm laser, followed by the OSL2 illuminator, whereas the 660 nm laser produced the weakest effect. Increasing ICG concentration further enhanced photodynamic cytotoxicity under constant irradiation conditions. The results demonstrate that PDT efficiency is strongly influenced by spectral overlap between the irradiation source and the absorption characteristics of the ICG-BSA complex, highlighting the importance of excitation conditions in determining photodynamic activity.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148930796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-activating factor-receptor signaling mediates solar-simulated light-induced extracellular vesicle release in human keratinocytes. 血小板活化因子受体信号传导介导太阳模拟光诱导的人角质形成细胞细胞外囊泡释放。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-06 DOI: 10.1111/php.70145

Divyanshu Aggarwal, Anita Thyagarajan, Michael G Kemp, Ravi P Sahu

{"title":"Platelet-activating factor-receptor signaling mediates solar-simulated light-induced extracellular vesicle release in human keratinocytes.","authors":"Divyanshu Aggarwal, Anita Thyagarajan, Michael G Kemp, Ravi P Sahu","doi":"10.1111/php.70145","DOIUrl":"https://doi.org/10.1111/php.70145","url":null,"abstract":"Ultraviolet (UV) radiation modulates keratinocyte biology through both cytotoxic and signaling-related mechanisms. However, the differential kinetics of UVA versus UVB in regulating extracellular vesicle (EV) release and the underlying mechanisms remain incompletely understood. In this study, we examined the effects of solar-simulated light (SSL; emitting both UVA and UVB) as well as UVA and UVB alone on human HaCaT keratinocytes, focusing on the role of platelet-activating factor-receptor (PAFR) and signaling in both large and small EV release. SSL and UVB induced dose- and time-dependent reductions in cell viability, whereas UVA caused minimal cytotoxicity at equivalent doses, indicating that the UVB component of SSL mediates cytotoxic responses. SSL significantly stimulated early release of large EVs (microvesicle particles, MVPs) and later release of small EVs (exosomes). Similar EV release effects were observed following UVB exposure, but not after UVA exposure. Pharmacological inhibition of PAFR significantly reduced SSL and UVB-induced release of both MVPs and exosomes. Furthermore, inhibition of acid sphingomyelinase (aSMase) or pretreatment with the antioxidant N-acetylcysteine significantly attenuated SSL/UVB-induced EV release. Collectively, these findings indicate PAFR- and aSMase-mediated signaling as key regulators of SSL-dependent keratinocyte EV release and provide mechanistic insight into how solar radiation influences intercellular communication in keratinocytes.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148901042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sun exposure and photoprotection: Habits, knowledge, and attitudes among elite beach soccer players. 阳光照射和光防护：海滩足球精英运动员的习惯、知识和态度。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-01 DOI: 10.1111/php.70148

Guillermo De Castro-Maqueda, Pau Vaccaro-Benet, Miguel Ángel Rosety Rodríguez, Macarena Rivero Vila

{"title":"Sun exposure and photoprotection: Habits, knowledge, and attitudes among elite beach soccer players.","authors":"Guillermo De Castro-Maqueda, Pau Vaccaro-Benet, Miguel Ángel Rosety Rodríguez, Macarena Rivero Vila","doi":"10.1111/php.70148","DOIUrl":"https://doi.org/10.1111/php.70148","url":null,"abstract":"Beach soccer players are exposed to prolonged ultraviolet radiation (UVR) due to the outdoor nature of the sport, minimal clothing coverage, and frequent participation in coastal environments with highly reflective surfaces. This study aimed to evaluate sun exposure habits, photoprotection practices, attitudes, knowledge related to skin cancer prevention, and the prevalence of sunburn episodes among elite beach soccer players. A cross-sectional observational study was conducted during the European Beach Soccer Qualifying Championship held in October 2025. A total of 257 elite male players from 21 European countries completed a standardized questionnaire assessing UVR exposure, photoprotection behaviors, sun-related attitudes, knowledge, and skin surveillance practices. Overall, 76% of athletes reported at least one sunburn during the previous sports season, and 35% experienced three or more episodes. Although 84% reported regular sunscreen use and 93% used products with a sun protection factor (SPF) ≥30, only 31% reported reapplying sunscreen every 2 h as recommended. Positive attitudes toward tanning remained common, while the overall knowledge score regarding skin cancer prevention was moderate (6.7 ± 1.6 points out of 10). In addition, 72% of players did not regularly perform skin self-examinations. These findings indicate that elite beach soccer players represent a high-risk population for UVR-related skin damage and highlight the need for targeted photoprotection and skin cancer prevention strategies.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148866522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional benchmark dose modeling of ultraviolet radiation-induced genomic activation in mouse skin. 紫外线辐射诱导小鼠皮肤基因组激活的转录基准剂量模型。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2026-09-01 Epub Date: 2025-11-24 DOI: 10.1111/php.70059

Sami S Qutob, Samantha P M Roesch, Sandy Smiley, Pascale V Bellier, Andrew Williams, Kate B Cook, Matthew J Meier, Andrea Rowan-Carroll, Carole L Yauk, James P McNamee, Vinita Chauhan

{"title":"Transcriptional benchmark dose modeling of ultraviolet radiation-induced genomic activation in mouse skin.","authors":"Sami S Qutob, Samantha P M Roesch, Sandy Smiley, Pascale V Bellier, Andrew Williams, Kate B Cook, Matthew J Meier, Andrea Rowan-Carroll, Carole L Yauk, James P McNamee, Vinita Chauhan","doi":"10.1111/php.70059","DOIUrl":"10.1111/php.70059","url":null,"abstract":"The in vivo transcriptional response of mouse skin to ultraviolet radiation (UV-R) exposure reveals key genomic alterations associated with UV-R-induced damage but it does not provide precise dose thresholds for these effects. These initial findings provided the impetus to advance dose-response characterization by integrating benchmark dose (BMD) modeling with transcriptomic data, aiming to identify biologically relevant points of departure for gene and pathway activation. To accomplish this, mice were exposed to five erythemally weighted UV-R doses (0-40 mJ/cm2) emitted from a UV-emitting tanning device, across six post-exposure timepoints (0-96 h). Four analytical methods were used to estimate BMDs, with the lowest consistent response dose (LCRD) approach yielding the most sensitive estimates (1.21-3.44 mJ/cm2). Transcriptomic responses revealed activation of shared pathways related to DNA damage and cancer, oxidative stress and metabolism, inflammation and immunity, and hormonal disruption. Notably, the majority of LCRD BMD estimates (1.21-3.44 mJ/cm2) were lower than the International Electrotechnical Commission standard actinic exposure limit (3 mJ/cm2 (erythemally weighted)) for broadband UV-R (200-400 nm) for unprotected skin and the eye for an 8 h period. These findings suggest that transcriptomic BMD modeling can detect early biological responses to UV-R at doses lower than current exposure limits.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1159-1168"},"PeriodicalIF":2.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13575721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0