Photochemistry and Photobiology最新文献_第6页

222 nm far-UVC light and skin health: Assessment of DNA damage across different skin types. 222nm远紫外线光与皮肤健康：不同皮肤类型的DNA损伤评估

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-09 DOI: 10.1111/php.70010

Manuela Buonanno, Camryn Petersen, David Welch, Raabia Hashmi, David J Brenner

{"title":"222 nm far-UVC light and skin health: Assessment of DNA damage across different skin types.","authors":"Manuela Buonanno, Camryn Petersen, David Welch, Raabia Hashmi, David J Brenner","doi":"10.1111/php.70010","DOIUrl":"https://doi.org/10.1111/php.70010","url":null,"abstract":"Due to a limited penetration into skin and eyes combined with a broad germicidal effectiveness, far-UVC light (200-235 nm) has been proposed as an effective intervention for airborne pandemic control. Specifically, 222 nm light is not predicted to damage skin because it is primarily absorbed by the proteins in the superficial stratum corneum of the epidermis. Thus, it is hypothesized that the thickness of the stratum corneum is one of the most significant contributing factors to the risk of skin damage from exposure to far-UVC. From measurements of the stratum corneum thickness in live human skin biopsies, it was found that none of the donor demographics studied had an impact on the thickness of the stratum corneum. While multiple studies suggest that exposure to 222 nm is minimally damaging to skin, a few studies to date have investigated effects as a function of skin characteristics (e.g., individual's age and sex). In selected tissues, the induction of DNA damage following an acute exposure to 100 or 500 mJ/cm2 from 222 nm light was analyzed as a function of donor demographics. The results agree with previous studies using other models of human skin and show that in human skin biopsies, 222 nm induces minor DNA damage only at high doses, especially in skin with low melanin content (phototype).","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory effects of photodynamic therapy for skin cancer: Potential strategies to improve treatment efficacy and tolerability. 光动力疗法对皮肤癌的免疫调节作用：提高治疗疗效和耐受性的潜在策略。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-04 DOI: 10.1111/php.70008

Mark P Ortenzio, Sanjay Anand, Jeffrey B Travers, Edward V Maytin, Craig A Rohan

{"title":"Immunomodulatory effects of photodynamic therapy for skin cancer: Potential strategies to improve treatment efficacy and tolerability.","authors":"Mark P Ortenzio, Sanjay Anand, Jeffrey B Travers, Edward V Maytin, Craig A Rohan","doi":"10.1111/php.70008","DOIUrl":"https://doi.org/10.1111/php.70008","url":null,"abstract":"Skin cancer, including melanoma and nonmelanoma variants, accounts for the majority of cancer diagnoses in the United States each year and is increasingly the focus of research on treatment and prevention. Photodynamic therapy (PDT) is a well-established, nonscarring treatment for nonmelanoma cancers such as early squamous precancers (actinic keratoses, AK), squamous carcinoma in situ, and superficial basal cell carcinoma (BCC). PDT can be painful during illumination and has relatively poor efficacy for thick lesions. However, these disadvantages could be ameliorated if newly recognized PDT effects on immunity were fully understood and harnessed. In this review, we highlight basic and translational studies on effects that locally administered PDT exerts on the immune system. On the one hand, PDT stimulates an anti-tumor immune response observable as peritumoral infiltration of innate and adaptive immune cells and activation of cytotoxic T cells both locally and in circulating blood. On the other hand, PDT generates systemic immunosuppression mediated by the release of subcellular microvesicle particles (MVP); the latter may be blocked by using acid sphingomyelinase (aSMase) inhibitors. The ultimate goal must be to balance two opposing forces, that is, to limit PDT-induced immunosuppression while promoting PDT-induced regression of tumors so as to favor a long-term anti-tumor immune response.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solar-driven photocatalytic system for CO₂ fixation and conversion of dopamine into indole derivative. 二氧化碳固定和多巴胺转化为吲哚衍生物的太阳能驱动光催化系统。

IF 2.6 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-02 DOI: 10.1111/php.70007

Rehana Shahin, Rajesh K Yadav, Rajesh K Verma, Shaifali Mishra, Chandani Singh, Jin-OoK Baeg

{"title":"Solar-driven photocatalytic system for CO2 fixation and conversion of dopamine into indole derivative.","authors":"Rehana Shahin, Rajesh K Yadav, Rajesh K Verma, Shaifali Mishra, Chandani Singh, Jin-OoK Baeg","doi":"10.1111/php.70007","DOIUrl":"https://doi.org/10.1111/php.70007","url":null,"abstract":"Photocatalytic CO₂ fixation into solar fuels offers a promising route for renewable energy storage by converting CO₂ into chemical bonds. Among various products, formic acid is considered the most reliable candidate for industrial applications due to its high efficiency and sustainable feasibility. Various catalysts, including metals, chalcogenides, transition metals, and carbon-based materials, have been explored for this purpose. Polymeric organic frameworks are a class of crystalline polymers with tunable structures, making them potential candidates for metal-free photocatalysts. However, their low crystallinity often limits light-harvesting efficiency and photocatalytic activity, posing a challenge for industrial applications. The primary obstacles in this field are low activity and poor selectivity of photocatalysts. In this study, we propose a soft-template induction strategy to construct a metal-free heterojunction polymeric framework based on 5,15-di-(4-aminophenyl)-10,20-diphenyl porphyrin (BP) and perylene tetra-anhydride (PT), referred to as PTBP. This polymer exhibits high crystallinity and strong solar light absorption. The PTBP framework demonstrates better performance in solar-powered molecular artificial photosynthesis, achieving significant improvements over PT. Specifically, PTBP exhibits high 1,4-NADH/NADPH regeneration efficiencies (52.51%/58.41%) compared to PT (9.11%/10.1%), a substantial NADH consumption (119.25 μmol) in exclusive solar fuel production from CO₂ within 1 h, and excellent yield (50.37%) in the photocatalytic conversion of dopamine into an indole-derivative, surpassing PT (13.93%). The current finding highlights the benchmark photocatalytic potential of the PTBP polymeric framework's capacity for photocatalysis for CO2 fixation and conversion of dopamine into indole derivatives.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144554156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced fluorescence emission or singlet oxygen production of cationic porphyrazines and porphyrins through combination with carbon dots. 阳离子卟啉和卟啉通过与碳点结合增强荧光发射或单线态产氧。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2025-05-21 DOI: 10.1111/php.14119

Gustavo Wander Streit, Rafael Bernardino Rodrigues da Silva Taques, Gabriela Fernandes Barreto, Fabiano Vargas Pereira, Gilson DeFreitas-Silva, Thiago Teixeira Tasso

{"title":"Enhanced fluorescence emission or singlet oxygen production of cationic porphyrazines and porphyrins through combination with carbon dots.","authors":"Gustavo Wander Streit, Rafael Bernardino Rodrigues da Silva Taques, Gabriela Fernandes Barreto, Fabiano Vargas Pereira, Gilson DeFreitas-Silva, Thiago Teixeira Tasso","doi":"10.1111/php.14119","DOIUrl":"10.1111/php.14119","url":null,"abstract":"A cationic porphyrin and porphyrazine with the 3-ethylpyridyl substituent (H2P and H2Pz) and their respective zinc complexes (ZnP and ZnPz) were assembled to a carbon dot (CD) synthesized from citric acid and ammonium citrate. A titration was performed using a fluorescence spectrophotometer to determine the stoichiometric ratio at which the maximum interaction between the substances occurs, as well as the Stern-Volmer constant and intrinsic binding constant. The combination between CD and porphyrins or porphyrazines was confirmed using UV-VIS absorption spectroscopy, fluorescence emission, zeta potential, and Diffusion-Ordered NMR Spectroscopy (DOSY). It was observed that after combination, there is a decline in the absorption of porphyrin derivatives, a variation in the emission of porphyrazines, a subtle increase in the zeta potential compared to the isolated CD particles, and a variation in the translational diffusion coefficient. It was also found that upon combination with the CD, changes in the photophysical properties of the macrocycles occur, for example, the fluorescence quantum yield of H2Pz increases from 0.81 ± 0.03% to 1.97 ± 0.05% while the singlet oxygen quantum yield of H2P increases ca. 70%. These results exemplify the capacity of CD to boost some properties of photosensitizers that are key for photodynamic therapy applications.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"987-999"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12345398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy atom effects on synthetic pyranoanthocyanin analogues. 重原子对合成吡喃花青素类似物的影响。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2024-12-26 DOI: 10.1111/php.14058

Lucas M O S Martins, Gustavo T M Silva, Lucas F S Hess, Alexandre B Barbosa, Claudia Turro, Mauricio S Baptista, Frank H Quina

{"title":"Heavy atom effects on synthetic pyranoanthocyanin analogues.","authors":"Lucas M O S Martins, Gustavo T M Silva, Lucas F S Hess, Alexandre B Barbosa, Claudia Turro, Mauricio S Baptista, Frank H Quina","doi":"10.1111/php.14058","DOIUrl":"10.1111/php.14058","url":null,"abstract":"Pyranoflavylium cations are synthetic analogues of pyranoanthocyanins, the much more color-stable compounds that are formed spontaneously from grape anthocyanins during the maturation of red wines. In the present work, our studies of the photophysical properties of pyranoanthocyanin analogues are extended to include nine pyranoflavylium cations substituted with one or two bromo and/or iodo heavy atoms. The room temperature fluorescence, 77 K fluorescence and phosphorescence, triplet formation in solution, and sensitized singlet oxygen formation, with excited state acidity suppressed by the addition of trifluoroacetic acid, are compared to those of similar pyranoflavylium cations that do not contain a heavy atom. Heavy atom effects on the photophysics of the S1 state of pyranoflavylium cations are found to be relatively small, which is attributed to the nodal properties of the orbitals involved, which prevent effective mixing of the spin-orbit coupling on the heavy atoms into the excited singlet state, S1, of the pyranoflavylium chromophore. Heavy atom effects on the phosphorescence of these heavy atom-substituted pyranoflavylium cations at 77 K are somewhat larger, consistent with a spin-orbit coupling-induced increase in the radiative rate constant for phosphorescence, as are the triplet-sensitized singlet oxygen formation quantum yields in fluid solution.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1039-1048"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of transcranial photobiomodulation on fine motor skills in boccia para-athletes. 经颅光生物调节对羽毛球运动员精细运动技能的影响。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2025-01-21 DOI: 10.1111/php.14060

Edna de Morais Garcez, Alessandra Baptista, Michael R Hamblin, Selly S Suzuki, Aguinaldo Garcez, Silvia Cristina Nunez, Ricardo S Navarro, Thamiris Bassoto, Aguinaldo Silva Garcez

{"title":"Effects of transcranial photobiomodulation on fine motor skills in boccia para-athletes.","authors":"Edna de Morais Garcez, Alessandra Baptista, Michael R Hamblin, Selly S Suzuki, Aguinaldo Garcez, Silvia Cristina Nunez, Ricardo S Navarro, Thamiris Bassoto, Aguinaldo Silva Garcez","doi":"10.1111/php.14060","DOIUrl":"10.1111/php.14060","url":null,"abstract":"This study investigated the effects of transcranial photobiomodulation (t-PBM) on para-athletes' manual dexterity and intralimbal coordination. Six para-athletes from a Boccia Team participated. t-PBM was administered using a LED helmet with 204 LEDs (660 and 850 nm) emitting 10 mW each, delivering 9 J/cm2 per LED during 15-min sessions three times a week. The trial lasted 2 weeks, with baseline, placebo treatment, and post-treatment assessments. Manual dexterity was measured by Box and Ball Test (BBLT), and intralimbal coordination by discrete and continuous horizontal and vertical touch tests. Results showed significant improvements in manual dexterity for the t-PBM group compared to the placebo. Discrete vertical touch tests showed reduced time to perform movements, and continuous vertical touch tests showed increased movement frequency in the t-PBM group. No significant improvements were observed in the placebo group. t-PBM was shown to be a potentially effective treatment, with significant benefits in fine motor skills.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"896-903"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toluidine blue O demethylated photoproducts as type II photosensitizers. 甲苯胺蓝O去甲基化光产物作为II型光敏剂。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2025-01-20 DOI: 10.1111/php.14066

Whitney Querini-Sanguillén, Jennifer Otero-González, Melannie García-Sánchez, Daniel Zúñiga-Núñez, Germán Günther, Mario L Miranda, Edgardo Castro-Pérez, Carlos Ramos, Denis Fuentealba, José Robinson-Duggon

{"title":"Toluidine blue O demethylated photoproducts as type II photosensitizers.","authors":"Whitney Querini-Sanguillén, Jennifer Otero-González, Melannie García-Sánchez, Daniel Zúñiga-Núñez, Germán Günther, Mario L Miranda, Edgardo Castro-Pérez, Carlos Ramos, Denis Fuentealba, José Robinson-Duggon","doi":"10.1111/php.14066","DOIUrl":"10.1111/php.14066","url":null,"abstract":"Toluidine blue O (TBO) is a type I-type II photosensitizer that has shown good efficacy and selectivity in antimicrobial and anticancer photodynamic therapy applications. However, its complex photochemistry with multiple photoproducts hinders its application as a photosensitizer. We have previously described the mechanism for photooxidative demethylation of TBO which in acetonitrile yields two main products: demethylated-TBO (d-TBO) and double-demethylated-TBO (dd-TBO). In the current work, we describe the photophysical properties of these two photoproducts. In acetonitrile and phosphate buffer, demethylation induces an hypsochromic shift in the absorption and fluorescence emission maxima. Fluorescence quantum yields increase slightly for the demethylated photoproducts, in agreement with the lengthening of the fluorescence lifetimes. Triplet excited states lifetimes in the presence of oxygen decreased slightly upon demethylation. However, the singlet oxygen quantum yield increased significantly reaching unity for the dd-TBO photoproduct. These results are interpreted in terms of the competing pathways of TBO photochemistry. For TBO, demethylation is the main pathway for deactivation of the excited state, while for d-TBO, demethylation and singlet oxygen generation are significant. For dd-TBO, singlet oxygen generation is the main deactivation pathway. Overall, TBO demethylated photoproducts demonstrate good potential as candidates for photodynamic therapy applications.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1061-1071"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting porphyrin synthesis and ALA-mediated photoinactivation through near-infrared therapy. 通过近红外治疗促进卟啉合成和ala介导的光失活。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2025-01-20 DOI: 10.1111/php.14056

Roberto Tomás, Gabriela Di Venosa, Daniel Sáenz, Fernanda Buzzola, Leandro Mamone, Adriana Casas

{"title":"Boosting porphyrin synthesis and ALA-mediated photoinactivation through near-infrared therapy.","authors":"Roberto Tomás, Gabriela Di Venosa, Daniel Sáenz, Fernanda Buzzola, Leandro Mamone, Adriana Casas","doi":"10.1111/php.14056","DOIUrl":"10.1111/php.14056","url":null,"abstract":"Photodynamic inactivation (PDI) combines the use of photosensitizers with visible light to produce reactive oxygen species that effectively eliminate pathogens. To investigate the impact of near- infrared therapy (NIRT) on heme biosynthesis and permeability of the pro-photosensitizers 5-aminolevulinic acid (ALA) and Hexyl-ALA (H-ALA) through biofilms, we applied sub-lethal conditions for both NIRT and PDI to maintain intact bacterial viability. During NIRT, the temperature remained below 37°C, permitting rapid heating (ΔT = 11°C) without causing thermal damage. NIRT potentiated the bactericidal effects of ALA-PDI by 3 logs in Staphylococcus aureus RN6390 biofilms and 4 logs in ST5-SCCmecI biofilms. With H-ALA-PDI, reductions of 4 and 6 logs, respectively, were observed. In the case of ALA, this enhancement was partly due to increased porphyrin synthesis, a result not replicated by simple heating. For H-ALA, the enhanced effect of PDI was likely due to biofilm or bacterial membrane destabilization caused by NIRT and H-ALA. Unlike biofilms, applying the same ALA-PDI conditions, the treatment was effective in planktonic S. aureus RN6390 cultures, reducing the bacteria by 3 logs, with no additional benefit from NIRT, suggesting that NIRT primarily aids in penetrating the biofilm matrix rather than the bacterial cells. Confocal fluorescence microscopy confirmed that NIRT-treated biofilms exposed to ALA exhibited higher porphyrin fluorescence than untreated biofilms. These findings support the use of NIRT in enhancing the effectiveness of PDI against bacterial biofilms.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"936-946"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative study of ergosterol and 7-dehydrocholesterol and their endoperoxides: Generation, identification, and impact in phospholipid membranes and melanoma cells. 麦角甾醇和7-脱氢胆固醇及其内过氧化物的比较研究：磷脂膜和黑色素瘤细胞的生成、鉴定和影响。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2025-01-21 DOI: 10.1111/php.14059

Megumi Nishitani Yukuyama, Karen Campos Fabiano, Alex Inague, Miriam Uemi, Rodrigo Santiago Lima, Larissa Regina Diniz, Tiago Eugenio Oliveira, Thais Satie Iijima, Hector Oreliana Fernandes Faria, Rosangela Silva Santos, Maria Fernanda Valente Nolf, Adriano Brito Chaves-Filho, Marcos Yukio Yoshinaga, Helena Couto Junqueira, Paolo Di Mascio, Mauricio da Silva Baptista, Sayuri Miyamoto

{"title":"Comparative study of ergosterol and 7-dehydrocholesterol and their endoperoxides: Generation, identification, and impact in phospholipid membranes and melanoma cells.","authors":"Megumi Nishitani Yukuyama, Karen Campos Fabiano, Alex Inague, Miriam Uemi, Rodrigo Santiago Lima, Larissa Regina Diniz, Tiago Eugenio Oliveira, Thais Satie Iijima, Hector Oreliana Fernandes Faria, Rosangela Silva Santos, Maria Fernanda Valente Nolf, Adriano Brito Chaves-Filho, Marcos Yukio Yoshinaga, Helena Couto Junqueira, Paolo Di Mascio, Mauricio da Silva Baptista, Sayuri Miyamoto","doi":"10.1111/php.14059","DOIUrl":"10.1111/php.14059","url":null,"abstract":"Melanoma is an aggressive cancer that has attracted attention in recent years due to its high mortality rate of 80%. Damage caused by oxidative stress generated by radical (type I reaction) and singlet oxygen, 1O2 (type II reaction) oxidative reactions may induce cancer. Thus, studies that aim to unveil the mechanism that drives these oxidative damage processes become relevant. Ergosterol, an analogue of 7-dehydrocholesterol, important in the structure of cell membranes, is widely explored in cancer treatment. However, to date little is known about the impact of different oxidative reactions on these sterols in melanoma treatment, and conflicting results about their effectiveness complicates the understanding of their role in oxidative damage. Our results highlight differences among ergosterol, 7-dehydrocholesterol (7-DHC), and cholesterol in membrane properties when subjected to distinct oxidative reactions. Furthermore, we conducted a comparative study exploring the mechanisms of cell damage by photodynamic treatment in A375 melanoma. Notably, endoperoxides from ergosterol and 7-DHC generated by 1O2 showed superior efficacy in reducing the viability of A375 cells compared to their precursor molecules. We also describe a step-by-step process to produce and identify endoperoxides derived from ergosterol and 7-DHC. While further studies are needed, this work provides new insights for understanding cancer cell death induced by different oxidative reactions in the presence of biologically relevant sterols.","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"960-978"},"PeriodicalIF":2.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic therapy of cancer-associated infections. 癌症相关感染的光动力治疗。

IF 2.5 4区生物学

Photochemistry and Photobiology Pub Date : 2025-07-01 Epub Date: 2024-12-31 DOI: 10.1111/php.14038

Giulia Kassab, Juan Chen, Gang Zheng

引用次数: 0