Photochemistry and Photobiology最新文献

{"title":"Effects of photobiomodulation with 450 nm and 635 nm laser wavelengths on the release of TGF-β, BMP-2, FGF-2, and VEGF from dental pulp stem cells.","authors":"Mahsa Sadeghi, Kiumars Nazarimoghadam, Hossein Labbaf, Zahra Jafari, Nasim Chiniforush","doi":"10.1111/php.70073","DOIUrl":"https://doi.org/10.1111/php.70073","url":null,"abstract":"<p><p>This study assessed the effects of photobiomodulation with 450 and 635 nm laser wavelengths on the release of transforming growth factor-beta (TGF-β), bone morphogenetic protein (BMP)-2, fibroblast growth factor (FGF)-2, and vascular endothelial growth factor (VEGF) from human dental pulp stem cells (DPSCs). In this in vitro study, human DPSCs were cultured and randomly assigned to two experimental groups to undergo 450 and 635 nm laser irradiation with 2, 4, and 6 J/cm² energy densities, and one control group (n = 5). After laser irradiation, total RNA was then extracted, cDNA was synthesized, and expression of TGF-β, FGF-2, BMP-2, and VEGF was assessed by real-time polymerase chain reaction (PCR). Data were analyzed by one-way and two-way ANOVA and Tukey's test (alpha = 0.05). The effects of laser wavelength, energy density, and their interaction were significant on the release of all growth factors (p < 0.0001). Laser irradiation in almost all subgroups increased the release of growth factors. By an increase in energy density, the release of BMP-2 and FGF-2 in both wavelengths, VEGF in the 635 nm group, and TGF-β in the 635 nm group increased while the release of VEGF in the 450 nm group decreased (p < 0.05). The amount of released growth factors in the 635 nm group was higher than in the 450 nm group (p < 0.05). PBMT with 450 and 635 nm lasers increased the release of TGF-β, BMP-2, FGF-2, and VEGF from human DPSCs in most energy densities, which may be of use in regenerative endodontics.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146053400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and parameters for the effectiveness of a UV-C device for infection control in dental clinics.","authors":"Kátia Cristiane Hall, Everton Granemann Souza, Chiara das Dores do Nascimento, Marcel Luiz Basso, Mário Lúcio Moreira, Carla Lucía David Pena, Wellington Luiz Oliveira da Rosa, Rafael Guerra Lund, Evandro Piva","doi":"10.1111/php.70065","DOIUrl":"https://doi.org/10.1111/php.70065","url":null,"abstract":"<p><p>Healthcare-associated infections (HAIs) remain a significant concern in dental settings, especially in light of increasing antimicrobial resistance. This study aimed to characterize and evaluate the efficacy of an ultraviolet C (UV-C) disinfection device specifically developed as an additional resource for dental clinics and healthcare settings. Colorimetric dosimeters were strategically distributed at different points and vertical levels to monitor UV-C radiation distribution in the environment. The emission spectrum and radiation intensity were measured using spectral measurement techniques, while microbiological analyses were conducted on seven commonly encountered surfaces in dental offices using mannitol salt agar selective for Staphylococcus aureus and Staphylococcus epidermidis. The results revealed a significant reduction in colony-forming units (CFUs), exceeding 90% at most sampling points (p < 0.05). An exponential falloff of irradiance with distance from the UV-C source was observed, highlighting the importance of proper positioning of the equipment. Notably, the UV-C device proved effective even on surfaces with higher microbial loads, such as the armrest of the dental chair. The findings demonstrate that both time and distance significantly affect disinfection efficacy, and that purpose-built UV-C devices are viable as complementary tools to conventional chemical cleaning and disinfection protocols.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a photoswitchable acaricidal agent by the integration of an azobenzene moiety with a bifenazate structure.","authors":"Yongchao Zhang, Zhi Qiao, Zimai Liu, Qi Yin, Xiaoyan Li, Zhong Li, Wen Fu, Xusheng Shao","doi":"10.1111/php.70074","DOIUrl":"https://doi.org/10.1111/php.70074","url":null,"abstract":"<p><p>Photopharmacology achieves precise photo controlled regulation of drug activity by utilizing the properties of photochromic ligands. In this study, a series of novel azobenzene-based photochromic ligands (ABB1-5) were designed and synthesized, and their biological activities were confirmed to be precisely regulated by light irradiation. Bioassays against larvae of Culex pipiens pallens showed that the activity of ABB1 was significantly enhanced after light exposure, with the median lethal concentration (LC₅₀) decreasing from 27.20 μg/mL before irradiation to 16.99 μg/mL after, a reduction of 10.21 μg/mL, yielding a difference coefficient of 1.60. Similarly, experiments on Tetranychus cinnabarinus demonstrated a remarkable decrease in acaricidal activity for ABB4 after irradiation, as evidenced by an increase in LC₅₀ from 7.01 to 18.40 μg/mL, an increase of 11.39 μg/mL, with a difference coefficient of 2.62. Notably, the light-induced differences in activity exhibited by the ABBs series against both C. pipiens pallens larvae and T. cinnabarinus not only validate the potential of photochromic ligands in the precise optical control of drug efficacy but also offer a novel strategy for delaying the development of resistance to acaricides in phytophagous mites.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of deposit homogeneity on sunscreen efficacy: A novel quantitative method using LC-OCT.","authors":"Guillaume Votte, Annie Bossiere, Grégoire Guyon, Antoine Montaux-Lambert, Nathalie Blanpain, Rita Touti, Heiva Le Blay, Angelina Roudot","doi":"10.1111/php.70068","DOIUrl":"10.1111/php.70068","url":null,"abstract":"<p><p>Performance of sunscreens is primarily optimized through the development of their organic or mineral filtering systems, that is, the core of their photoprotective action. However, numerous studies have underscored that the homogeneity of the applied deposit is also key to optimize the effectiveness of a given filtering systems. Quantifying the homogeneity of a coating presents a significant challenge, particularly on complex substrates such as skin. Therefore, there is a key challenge in being able to have robust methods to measure deposit homogeneity for both in vitro and in vivo ultraviolet tests. This work focused on developing new quantitative methods for evaluating sunscreen deposit and its homogeneity. Our approach involved adaptation line-field confocal optical coherence tomography, a technique commonly used in biology, to precisely assess the thickness distribution of sunscreen deposit. For this study, we specifically investigated deposit modifications without changing the filtering system, thereby successfully linking variations in deposit thickness and homogeneity directly to filtration performances. This new method not only can be leveraged to advance the development and improvement of new photoprotective technologies but also paves the way for a more comprehensive understanding and characterization of sunscreen applications, both in vivo and in vitro, leading to improved protection.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of constitutive nitric-oxide synthase in regulation of IKKα after ultraviolet irradiation.","authors":"Yuxi Zhou, Lingying Tong, Bernardo Bastidas, Tao Liu, Madison Wright, Muxiang Zhou, Shiyong Wu","doi":"10.1111/php.70069","DOIUrl":"10.1111/php.70069","url":null,"abstract":"<p><p>Previously, we reported that UVB irradiation significantly reduces IKKα mRNA levels, while IKKα protein levels remain stable, a phenomenon maintained by constitutive nitric oxide synthase (cNOS) and NF-κB activity. In this study, we systematically investigated the transcriptional regulation of IKKα in response to UVB, with a focus on the role of cNOS. Using a series of luciferase reporter constructs containing deletions and site-specific mutations in the IKKα promoter, we evaluated promoter activity in HEK293 cells (cNOS-null) and HEK293cNOS cells (stably expressing cNOS). Our data identified two regulatory elements critical for UVB-inducible IKKα promoter activity: the second p53-binding site and the Ets-1 site. cNOS overexpression enhanced both basal and UVB-induced promoter activities in a dose-dependent manner. Interestingly, the promoter region spanning -940 to -438 harbors a repressive element that limits IKKα transcription. Although UVB activates the IKKα promoter, as shown by luciferase activity, it simultaneously inhibits transcriptional elongation. This likely explains the paradoxical reduction in endogenous IKKα mRNA levels. The effect is not due to decreased mRNA stability, highlighting transcriptional elongation as a key regulatory bottleneck. In parallel, in vivo studies using SKH-1 mice chronically exposed to solar-simulated UV (sUV) showed that cNOS knockout mice developed more tumors and exhibited significantly reduced IKKα expression compared to wild-type controls. These results demonstrate that cNOS regulates IKKα at multiple levels-promoter activation, transcriptional elongation, and protein stability. This multilayered control enhances our understanding of UV-induced skin pathogenesis and supports cNOS-IKKα signaling as a potential target for therapeutic intervention in skin cancer.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12952555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145912877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photophysics of hydroxy-nitrobenzoic acid UV-MALDI matrices investigated via spectroscopic, photoacoustic, and luminescence techniques.","authors":"Oscar E Silva Rodriguez, Gabriela Petroselli, Pedro M David Gara, Hernán B Rodríguez, Rosa Erra-Balsells","doi":"10.1111/php.70070","DOIUrl":"https://doi.org/10.1111/php.70070","url":null,"abstract":"<p><p>The photophysical behavior of novel UV-MALDI matrices belonging to the family of hydroxy-nitrobenzoic acids (HNBAs) was investigated in both acetonitrile and methanol solutions, as well as in the solid state, using spectroscopic, luminescence, and photoacoustic techniques. The study focused on the following positional isomers: 2-hydroxy-3-nitrobenzoic acid, 2-hydroxy-4-nitrobenzoic acid, 2-hydroxy-5-nitrobenzoic acid, 3-hydroxy-2-nitrobenzoic acid, 3-hydroxy-4-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, and 5-hydroxy-2-nitrobenzoic acid. Most isomers exhibited strong solid-state absorption at the excitation wavelengths commonly used in UV-MALDI technique (>90% at 337 and 355 nm), high photostability, no detectable luminescence, and highly efficient excitation energy dissipation as prompt heat, resembling the behavior of calorimetric references under both N₂- and air-saturated conditions, suggesting a potential role in facilitating desorption/ionization processes in UV-MALDI. These findings provide valuable insight into the performance of HNBAs as matrix candidates.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145906302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic reduction of nitro compounds using TiO₂-NaAsc-NC (LMCT/EDA) complex system in water under visible blue and green LED irradiation.","authors":"Ayub Ahmadi, Hadi Yari, Yadollah Shariati, Milad Taghizadeh, Foad Kazemi, Babak Kaboudin","doi":"10.1111/php.70062","DOIUrl":"https://doi.org/10.1111/php.70062","url":null,"abstract":"<p><p>In this study, we report a novel, eco-friendly, convenient reduction method of nitroarene compounds using TiO₂/PEG 400/sodium ascorbate (NaAsc). In this investigation, for the first time, we used sodium ascorbate to reduce nitroarene (NC) compounds which have significantly increased the efficiency of the reduction process through a proposed triad system (TiO₂/PEG 400/NaAsc). The reaction also progressed well in the presence of two sources of blue and green LED light, and the reduction of other nitroarene compounds under optimal conditions was performed with good to excellent efficiency.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145893227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The photobiomodulation effects of continuous and pulsed blue diode laser on proliferation and osteogenic differentiation of periodontal ligament stem cells.","authors":"Mohammadreza Karimi, Mahsa Sheibani Pour, Ardavan Etemadi, Mohammad Amin Karimi, Mahshid Hodjat, Nasim Chiniforush","doi":"10.1111/php.14104","DOIUrl":"10.1111/php.14104","url":null,"abstract":"<p><p>This study investigated the photobiomodulation effect of pulsed and continuous blue diode laser on osteogenic differentiation and proliferation of periodontal ligament mesenchymal stem cells. Periodontal Ligament Stem cells were seeded in 96-well plates, and 450 nm blue laser irradiation procedure was performed a day after cell seeding. Each experimental group was divided into two subgroups according to their energy density and irradiation duration: Continuous wave (100 mW, 10s, 2 J/cm² and 100 mW, 20 s, 4 J/cm²) and pulse wave (200 mW, 10 s, 2 J/cm² and 200 mW, 20 s, 4 J/cm² and duty cycle 50% for both). Then, all groups were evaluated with a cell viability test (MTT), cell apoptosis (Annexin V) on the second and fourth days after irradiation, Alizarin Red staining on the 14th day after irradiation based on genes. Real-time PCR was conducted 7 and 14 days after irradiation. GAPD gene primers were used as internal control, and OPN, OCN, ALP, and RUNX2 gene primers were used as tests. The one-way ANOVA statistical analysis revealed that cell proliferation in the continuous-irradiated groups was significantly higher than in pulsed groups. However, there is no significant difference in comparison with the control group. Also, pulsed-irradiated groups demonstrated a higher rate of necrosis. The osteogenic differentiation in the continuous groups was more substantial than in the pulsed and the control groups. In comparison to all other study groups, the group that received continuous mode irradiation at an energy density of 2 J/cm², power of 100 mW, and a radiation time of 10 s exhibited significantly higher numbers of calcified nodules and increased expression of OPN, OCN, and ALP genes (p < 0.05). Overall, treating periodontal ligament stem cells with a continuous blue diode laser and appropriate parameters can enhance their osteogenic differentiation and proliferation, accelerating the regeneration of periodontal tissues.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"198-206"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming resistance of Candida albicans using photodynamic inactivation.","authors":"Gabriela Gomes Guimarães, Jennifer Soares, Anna Luiza Resende, Isabella Gonçalves, Kate Blanco, Vanderlei Bagnato","doi":"10.1111/php.14108","DOIUrl":"10.1111/php.14108","url":null,"abstract":"<p><p>The increasing resistance to conventional antifungal agents, such as Amphotericin B (AmB), has led to a growing demand for alternative therapeutic approaches for Candida albicans, an opportunistic fungal pathogen responsible for infections in immunocompromised patients. This study aimed to evaluate the effectiveness of photodynamic inactivation (PDI) in combination with AmB for controlling C. albicans growth, particularly in its yeast and hyphal forms, and to assess the impact of multiple PDI doses. C. albicans (ATCC 90028) was cultured in yeast and hyphal suspensions that were adjusted to 10⁸ CFU/mL and treated with AmB at varying concentrations (0.065-1.04 μg/mL), with and without PDI. PDI was performed using the photosensitizer curcumin (2.5 μM), activated by a 450 nm LED light source at a fluence of 15 J/cm². The effect of single and repeated PDI doses was evaluated in the fungal biomolecules, which were assessed using Fourier transform infrared (FTIR) spectroscopy. Optical density (OD) measurements quantified fungal growth reduction at 540 nm. The combination of AmB and PDI significantly reduced C. albicans growth, achieving a 75% reduction in the yeast form and an 87.5% reduction in the hyphal form. Two doses of PDI further enhanced antifungal efficacy, particularly against hyphae, which exhibited higher sensitivity to treatment. These findings suggest that PDI enhances the antifungal action of AmB, particularly in more resistant C. albicans forms such as hyphae and biofilms. The observed synergistic effect supports the potential use of PDI as an effective strategy to combat antifungal resistance in clinical applications.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"127-140"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144079446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation associated with physical exercise in shoulder impingement syndrome. Systematic review with meta-analysis.","authors":"Giulia de Lara Quagliotto, Milena Pastor Manchope, Rebeca Hilario, Vitoria Zubeldia, Renata Anddresa Stacheslki, Alberito Rodrigo de Carvalho, Márcia Rosângela Buzanello, Gladson Ricardo Flor Bertolini","doi":"10.1111/php.14113","DOIUrl":"10.1111/php.14113","url":null,"abstract":"<p><p>To present the use of PBM associated with physical exercise in the treatment of shoulder impingement syndrome, with its different parameters. The following databases were used: PubMed, Web of Science, Embase, Cochrane, Scopus, and Lilacs. The gray literature included Google Scholar, Open Grey, LIVIVO, and the Brazilian Library of Theses and Dissertations. Ten randomized clinical trials were included. Pain intensity was improved with PBM compared to control [MD = -0.89, 95% CI (-1.38, -0.40), I² 46%, p = 0.0004]. The 3 different times of assessment (at rest, activity, and at night) of pain intensity were not statistically significant and likewise, the combined effect size [SMD = -0.16; 95% CI (-0.43, 0.12), I² 63%, p = 0.26]. A significant improvement in ROM was seen in the PBM group compared to the control [MD = 12.24, 95% CI (7.64, 16.84), I² 85%, p < 0.00001]. The combined effect estimate showed no significant improvement in functionality in the PBM group compared to the control [MD = -1.47, 95% CI (-7.34, 4.41), I² 58%, p = 0.62]. PBM therapy combined with physical exercise is effective in reducing pain and improving the range of motion in individuals with shoulder impingement syndrome.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"220-236"},"PeriodicalIF":2.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12807334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}