Photochemistry and Photobiology最新文献

{"title":"Apoptosis kinetics of purified and PBMC-associated lymphocytes after photopheresis-like 8-methoxypsolaren/UVA treatment.","authors":"Carlos Agustin Villegas-Valverde, Imilla Casado Hernandez, Yandy Marx Castillo-Aleman, Gisela Maria Suarez-Formigo, Amar Riaz, Antonio Alfonso Bencomo-Hernandez, Yendry Ventura-Carmenate","doi":"10.1111/php.70021","DOIUrl":"https://doi.org/10.1111/php.70021","url":null,"abstract":"<p><p>Studies of apoptosis kinetics in extracorporeal photopheresis (ECP) contribute to identifying modifications that can positively impact the configuration of ECP offline in a clinical setting. This work compared for the first time the apoptosis kinetics of human peripheral blood mononuclear cell (PBMC)-associated T lymphocytes versus the isolation of high-purity T cells using the MacoGenic G2 system, and times with predictive value were identified through ROC-AUC analysis, which facilitates quality control of the ECP. PBMC and T lymphocytes from healthy donors were exposed to 300 ng/mL of 8-methoxypsoralen combined with 2 J/cm² of ultraviolet A light (320-400 nm) using the MacoGenic G2 system. The cells were then cultured in vitro, and immunophenotyping and apoptosis induction were assessed by flow cytometry analysis. Apoptosis levels in monocyte controls reached 47% at 24 h, while treated monocytes maintained high apoptosis at 90.4%. Both PBMC-associated lymphocytes and isolated T cells exhibited sigmoidal apoptosis kinetics; however, PBMCs exhibited a lower apoptosis percentage (65.2%) compared with isolated T cells (73.8%); this difference was not statistically significant (p = 0.8229). Early apoptosis was faster in NKT, NK, and Tc cells, while B and Th cells exhibited slower kinetics, peaking at 24 h. Isolated T cells showed a faster early apoptosis peak after irradiation (baseline measure), with NKT cells most affected. AUC analysis at 24 h demonstrated high predictive accuracy for apoptosis in PBMCs (AUC = 0.97, p = 0.0004) and isolated T cells (AUC = 1, p = 0.0003). Nearly all treated PBMC-associated lymphocytes and isolated T lymphocytes underwent apoptosis within 72 h. Apoptosis kinetics were comparable, though slightly accelerated in T lymphocytes. Predictions of ECP effects showed high sensitivity and specificity at 24 h for PBMCs and at 4 h after treatment (baseline) for isolated T lymphocytes, with distinct apoptosis susceptibility patterns.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144848277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of electric fields on bioluminescence emissions of fireflies.","authors":"Amlan Jyoti Borah, Anurup Gohain Barua","doi":"10.1111/php.70019","DOIUrl":"https://doi.org/10.1111/php.70019","url":null,"abstract":"<p><p>Light emissions from the firefly at different temperatures and in magnetic fields were studied in recent times. Changes were observed in the wavelength peak position and flash duration in those studies, giving information on the light-producing reaction and the system in the live firefly. Here we report emissions from the firefly species Abscondita perplexa (Walker, 1858) placed in an electric field. The AC electric field is not found to have any effect, but the DC field causes an instantaneous increase in the duration of the flashes. These results suggest that the insect experiences a cooling type of stress in the static electric field-probably a result of becoming positively charged-while staying immune to the AC field.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Database of low-temperature absorption and fluorescence spectra of native photosynthetic tetrapyrrole macrocycles.","authors":"Dariusz M Niedzwiedzki, Masahiko Taniguchi, Jonathan S Lindsey","doi":"10.1111/php.70018","DOIUrl":"https://doi.org/10.1111/php.70018","url":null,"abstract":"<p><p>Low-temperature (77 K) absorption and fluorescence spectra of 12 naturally occurring photosynthetic tetrapyrrole macrocycles have been recorded in a frozen glass (2-methyltetrahydrofuran). The compounds encompass distinct chromophore classes: porphyrin, chlorophyll c₂; chlorin, chlorophylls a, b, d, f and bacteriochlorophylls c, d, e, f; and bacteriochlorin, bacteriochlorophylls a, b, g. The spectra are compared with those of the same pigment in liquid solution (predominantly 2-methyltetrahydrofuran) at room temperature (293 K). The measured Stokes shifts at 77 K across the 12 macrocycles range from ~30 to 300 cm^-1. The spectral data in digital form are made available as part of the PhotochemCAD databases. Literature searches have revealed extensive published data for Chl a (often in biological matrices) but at best rather limited data for less common macrocycles. The availability of a systematic collection of curated spectral data collected at low temperature should be useful for a variety of assessments, including reconstruction of absorption spectra of (bacterio)chlorophyll-containing protein complexes, vibrational analysis of absorption and fluorescence spectra, and calculations where knowledge of energy levels is important.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photochemical construction of chromanone-fused estrones using a biphasic tandem rearrangement-cyclization approach.","authors":"Matías I Quindt, Gabriel F Gola, Javier A Ramirez, Sergio M Bonesi","doi":"10.1111/php.70017","DOIUrl":"https://doi.org/10.1111/php.70017","url":null,"abstract":"<p><p>A facile photochemical preparation of 4-chromanone fused to estrone has successfully been achieved upon direct irradiation with light of 254 nm under a nitrogen atmosphere employing 3-(2'-alkenoyl)estrone and 3-(2'-alkenoyl)-17-norestrone derivatives as optimal substrates. The two-phase acid- and base-catalyzed method relies upon two consecutive pathways in a one-pot fashion, involving the photo-Fries rearrangement reaction and a catalyzed intramolecular oxa-Michael addition to afford the desired 4-chromanone fused products in good yields.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144765198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human adipose mesenchymal stem cell derived extracellular vesicles-delivered HSP27 alleviates UVB-induced photoaging.","authors":"Qianwen Zeng, Rentao Yu, Genlong Bai, Qian Wu, Bo Chen, Aijun Chen","doi":"10.1111/php.70015","DOIUrl":"https://doi.org/10.1111/php.70015","url":null,"abstract":"<p><p>Skin photoaging is a skin condition caused by long-term exposure to ultraviolet radiation, especially UVA and UVB, which leads to wrinkles, pigmentation, skin sagging, and telangiectasia. Histopathologically, it is characterized by a significant reduction in dermal collagen and abnormal accumulation of elastic fibers. Preventing or ameliorating photoaging may provide a promising therapeutic approach for these changes. In recent years, multiple studies have reported the potential of mesenchymal stem cells (MSCs) in treating various skin diseases. Given that extracellular vesicles (EVs) can deliver diverse substances to receptor cells and produce therapeutic effects similar to parental cells, we aim to explore whether adipose-derived mesenchymal stem cell-derived extracellular vesicles (AMSC-EVs) can improve skin photoaging by delivering heat shock protein 27 (HSP27). The specific effects of AMSC-EVs on the photoaging model of human dermal fibroblasts (HDFs) or human immortalized keratinocytes (HaCaTs) induced by UVB irradiation were investigated through CCK-8 experiments, cell migration experiments, flow cytometry, immunofluorescence, and Western blot. Our research found that AMSC-EVs improved the survival rate and migration ability of HDFs and HaCaTs after UVB irradiation, alleviated cell senescence, reduced DNA damage, inhibited the production of ROS, and promoted the remodeling of extracellular matrix (ECM). Further research showed that after knocking down HSP27, the anti-aging/light protection ability of AMSC-EVs was significantly weakened. Overall, our data suggest that we have revealed the anti-photoaging effect of AMSC-EVs on HDFs and HaCaTs, which may be mediated by the delivery of HSP27.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144744167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germicidal potential and skin compatibility of an innovative UVC phototherapy device emitting at 234 nm.","authors":"Zheng Tang, David Welch, Manuela Buonanno, Mark Gerber, David J Brenner","doi":"10.1111/php.70016","DOIUrl":"https://doi.org/10.1111/php.70016","url":null,"abstract":"<p><p>Chronic wounds are a major healthcare issue affecting more than 10 million Americans each year, with a 5-year survival similar to cancer and costing the healthcare system billions of dollars annually. Current solutions, such as antiseptics and antibiotics, can be toxic to cells or contribute to the development of antibiotic-resistant strains of bacteria. Exposure to germicidal ultraviolet radiation (GUV) at 254 nm has been reported as an effective method for chronic wound management. However, concerns about the health hazards from exposure to 254 nm radiation have limited its use for wound management applications. In contrast, wavelengths of ultraviolet radiation in the range of 200-235 nm have exhibited similar germicidal ability but with a lower penetration range in tissue, potentially making those wavelengths better suited for chronic wound disinfection. In this study, a novel phototherapy device emitting principally at 234 nm was used to assess the killing efficacy against Methicillin-resistant Staphylococcus aureus (MRSA). Potential health hazards from exposure using the device were evaluated using a 3D human skin model. The ultraviolet exposure device tested in this study shows promise for effective decontamination of chronic nonhealing wounds without associated health hazards.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting pain and inflammation: A comparative study of photobiomodulation with 532 and 660 nm lasers in rats.","authors":"Andréa Ribeiro Mattoso-Câmara, Juliana Zampoli Boava Papini, Marcos Aurélio Teixeira, Denise Nami Fujii, Giovana Radomille Tofoli, Aguinaldo Silva Garcez","doi":"10.1111/php.70013","DOIUrl":"https://doi.org/10.1111/php.70013","url":null,"abstract":"<p><p>This study evaluated the analgesic and anti-inflammatory effects of photobiomodulation (PBM) using 532 nm (green) and 660 nm (red) low-power lasers in an animal model of acute postoperative pain. Forty-five Wistar rats underwent a 1 cm surgical incision on the right hind paw and were randomly assigned to three groups: red laser (RL, 660 nm, 100 mW, 5 J, 167 J/cm²), green laser (GL, 532 nm, 70 mW, 4.97 J, 166 J/cm²), and control (LO, no irradiation). PBM was applied immediately and at 1-, 3-, 6-, and 24-h postsurgery, and pain levels were assessed using von Frey's electronic analgesimeter. Inflammatory cytokines (TNF-α, IL-1β, CGRP, and Substance P) were measured by ELISA. Results showed that both RL and GL were significantly more effective than the control group in reducing pain and inflammation. RL provided superior analgesia, increasing pain tolerance to 690.54 ± 50.20 g at 24 h, reaching levels comparable to the non-incised paw (p < 0.001). GL demonstrated greater anti-inflammatory effects, significantly reducing TNF-α levels at 1 h (p < 0.05) and 24 h and maintaining lower IL-1β and CGRP levels. RL also modulated Substance P levels, correlating with its stronger analgesic effect. These findings suggest that RL is preferable for direct pain relief, while GL is more effective in modulating inflammatory responses. Given the statistically significant improvement in pain control and inflammatory marker modulation, PBM using these wavelengths could be a valuable adjunct therapy for postoperative pain management and enhanced healing in surgical patients. Future studies should explore synergistic PBM protocols combining both wavelengths to optimize clinical outcomes.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent proteins: A journey from the cell to extreme environments in material science.","authors":"Bianca Menchicchi, Andre C Stiel, Mattia Nieddu, J P Fuenzalida-Werner","doi":"10.1111/php.70011","DOIUrl":"https://doi.org/10.1111/php.70011","url":null,"abstract":"<p><p>This review presents the progression from the use of fluorescent proteins (FPs) and chromoproteins as bioimaging labels and sensors to the strategic engineering of their properties for robust functionality in synthetic and non-biological environments. Specifically, engineered variants of the small ultra-red fluorescent protein (smURFP) were developed and optimized for optoacoustic imaging through structure-guided mutagenesis. Reversibly switchable genetically encoded indicators were also created to enhance bioimaging capabilities. To extend the applicability of such proteins to material science and enable their function in everyday applications-such as environmental sensors, encoders, or color components in textiles and electronics-their inherent stability limitations were addressed. For this purpose, supramolecular stabilization strategies, including genetically encoded macro-oligomerization techniques, were explored. These methods effectively enhanced the resilience of FPs under chemically challenging conditions, without compromising their photophysical properties. Finally, the exploration of circularly polarized luminescence (CPL) from FPs is discussed, and their potential as CPL emitters suitable for sustainable photonic applications is identified. Overall, the transformative potential of engineered FPs as essential components for applications beyond bioimaging is emphasized.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of pharmacotherapeutic activity of antibiotic \"Amphotericin B\" due to its photosensitizing properties.","authors":"Nadezhda I Schastnaya, Tatyana E Kuznetsova, Hanna E Pyzh, Aliaksandr V Mikulich, Antonina I Tretyakova, Tatsiana S Ananich, Ludmila G Plavskaya, Vitaly Yu Plavskii","doi":"10.1111/php.70014","DOIUrl":"https://doi.org/10.1111/php.70014","url":null,"abstract":"<p><p>The problem of increasing the efficacy of fungal infection treatment is a pressing issue in modern medicine due to increasing resistance of pathogenic microorganisms to antifungal drugs. At the same time, the development of new antifungal drugs is a complex, lengthy, and expensive task, since there is a close evolutionary relationship between fungal eukaryotes and human somatic cells. The aim of this work is to enhance the pharmacotherapeutic activity of the widely used antifungal polyene antibiotic \"Amphotericin B\". As a result of in vitro studies using somatic cells, the ability of Amphotericin B to act as a photosensitizer when exposed to light in the blue spectral region, corresponding to the absorption band of the drug, as well as to sensitize the Type II photochemical reactions (generation of singlet oxygen) and radical processes (Type I reactions) was demonstrated. When modeling contact dermatitis on depilated areas of rat skin, it was found that the combined use of Amphotericin B and radiation from super-bright LEDs with a wavelength of λ_max = 405 nm enhances the fungicidal effect of the drug and also causes a reliable decrease in the characteristic signs of dermatitis compared to the effect of each of the specified factors separately. The results obtained can find wide application in medical practice for the treatment of fungal lesions of the skin, oral cavity, female genital area, and so forth. The presence of photosensitizer (Amphotericin B), approved for use, and phototherapeutic equipment corresponding to its absorption spectrum make it possible to develop the necessary medical technologies.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polo-like kinases and UV-induced skin carcinogenesis: What we know and what's next.","authors":"Tanya Jaiswal, Durdana Muntaqua, Gagan Chhabra, Nihal Ahmad","doi":"10.1111/php.70002","DOIUrl":"10.1111/php.70002","url":null,"abstract":"<p><p>Prolonged and chronic exposure to UV radiation is a risk factor for multiple skin cancers. As the incidence of UV-associated skin cancers continues to rise, there is a pressing need for a deeper understanding of the underlying mechanisms driving these pathologies. Polo-like kinases (PLKs), a family of enzymes consisting of five members (PLK1-PLK5), have been implicated in various aspects of skin carcinogenesis. The inhibition of PLKs is currently being explored as a potential strategy for cancer management. While much of the research has predominantly concentrated on PLK1, recent studies are increasingly shedding light on the role of other PLK family members, given their growing importance in cancer progression. Understanding the relationship between UV-associated skin cancers and PLKs could open new avenues for more effective management of skin cancers. In this review, we discuss the critical mechanisms associated with UV and PLKs in causing skin cancers, followed by the potential role of UV in modulating PLKs in different skin cancers. We also examine the prospect of targeting PLK signaling to enhance therapies for UV-induced skin cancer and improve patient responses. So far, there is not enough literature focused on the simultaneous effects of PLKs and UV using skin cancer models, emphasizing the need for further research to completely understand the role of PLKs in UV-induced skin carcinogenesis.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12410275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}