Photochemistry and Photobiology最新文献

{"title":"Treatment planning evolution: Comparing approaches in photodynamic and radiation therapies.","authors":"Tina Saeidi, Azin Mirzajavadkhan, Lothar Lilge","doi":"10.1111/php.14071","DOIUrl":"10.1111/php.14071","url":null,"abstract":"<p><p>The setups of previous and ongoing clinical trials are based on prescribed PDT doses, PS concentration, and light intensity derived from averages of previous clinical or study populations. It is understood that monitoring of personalized PS and light dose is needed to improve PDT outcomes. Monitoring of photophysical, photochemical, or cytotoxic moieties is common, representing concepts of delivered, absorbed, or equivalent doses similar to those used in radiation therapy (RT). Unlike RT, these dose concepts are not equally well developed and standardized across the PDT clinical indications; however, there is potential to improve PDT treatment setup, planning, and delivery by leveraging methodologies from RT. This review summarizes dose definitions and advancements in RT treatment planning and presents the equivalent dose concepts for PDT, particularly how these concepts can expand on the existing methods for PDT treatment planning. By identifying the major limitations and areas for improvement in PDT planning, the hope is to stimulate preclinical and clinical research studies that can enhance the efficacy of PDT, improving patient outcomes.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":"1100-1119"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12466109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal and photoinduced chemistry of the aromatic Criegee intermediate, benzaldehyde oxide: Implications for indoor air quality.","authors":"Lily M Guidry, Kailyn A Zeringue, Tanima Barua, Michael F Vansco, Barbara Marchetti, Tolga N V Karsili","doi":"10.1111/php.70029","DOIUrl":"https://doi.org/10.1111/php.70029","url":null,"abstract":"<p><p>Essential oils contain a complex mixture of volatile organic compounds, ranging from terpenes to aromatics. When released into the indoor air environment or into the atmosphere, they may undergo oxidation to generate complex reactive intermediates that affect indoor air quality. Cinnamaldehyde is one such aromatic molecule that is abundant in essential oils. When released into the indoor air environment, it may undergo oxidation to form a carbonyl oxide (Criegee intermediate) with an aromatic substituent: benzaldehyde oxide. In this manuscript, we present a high-level quantum chemical study that shows that, unlike smaller atmospherically relevant Criegee intermediates, benzaldehyde oxide is expected to undergo solar photolysis on timescales that are competitive with its ground state unimolecular and bimolecular chemistry. We show that aromatic substitution leads to a drastic bathochromic shift in the spectroscopically relevant excited states, revealing that photolysis in the indoor or outdoor environment should not be neglected when modeling the climate and air quality implications of Criegee intermediates with extended conjugation. We predict a range of products that may be important for forming lower volatility compounds via tropospherically relevant photochemistry. To motivate future experimental validation of our results, we propose a viable synthetic procedure of the relevant precursor for generating and stabilizing benzaldehyde oxide.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Constitutive nitric oxide synthases deficiency impairs cyclobutane pyrimidine dimer repair following solar UV exposure in cells and mice.","authors":"Veronica Bahamondes Lorca, Yuxi Zhou, Christina Athans, Hailey Payne, Madison Wright, Zeinab Feyyaz, Lingying Tong, Dawn L Sammons, Shiyong Wu","doi":"10.1111/php.70024","DOIUrl":"https://doi.org/10.1111/php.70024","url":null,"abstract":"<p><p>Solar ultraviolet (sUV) radiation is a major environmental factor that induces DNA damage, promoting skin aging and carcinogenesis. The formation of cyclobutane pyrimidine dimers (CPDs) is one of the most prevalent forms of UV-induced DNA lesions, playing a central role in skin photocarcinogenesis. Constitutive nitric oxide synthase (cNOS), responsible for basal nitric oxide (NO^˙) production, has been implicated in various cellular processes, including the DNA damage response. However, the role of cNOS in modulating DNA repair post-UV exposure has not been explored. In this study, we investigated the impact of cNOS deficiency on CPD repair following sUV exposure using both in vivo and in vitro models. SKH-1 hairless wild-type and nNOS^+/-/eNOS^-/- (cNOS-deficient) mice were chronically exposed to sUV, revealing significantly exacerbated skin lesions in cNOS-deficient animals. Primary fibroblasts and skin explants derived from these mice, as well as HEK293 cells with stable cNOS overexpression, were analyzed for CPD formation and repair dynamics. Our findings show that cNOS knockout leads to impaired CPD repair, with CPD levels persisting longer in cNOS-deficient cells and tissues compared with wild-type controls. Reintroduction of cNOS expression in HEK293 cells accelerated CPD clearance early post-sUV exposure, suggesting a protective role for cNOS in the DNA repair process. These results highlight cNOS as a critical modulator of UV-induced DNA damage repair and underscore its potential role in mitigating skin carcinogenesis.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hardening-off under fluctuating light enhances the photosystem I resistance to excessive fluctuating light in Enhalus acoroides.","authors":"Xingkai Che, Yuchen Ye, Bin Zhou, Haixing Wu, Shuchen Liu, Hu Li, Jianguo Liu, Zhendong Li, Yongfu Li","doi":"10.1111/php.70028","DOIUrl":"https://doi.org/10.1111/php.70028","url":null,"abstract":"<p><p>Seagrass meadows of Enhalus acoroides are rapidly declining. Transplanting artificially cultured seedlings is considered an efficient method of seagrass restoration. However, excessive fluctuating light (eFL) in the wild is highly likely to be a major threat to the survival of transplanted seedlings of E. acoroides. This study demonstrated that eFL in the wild inhibited the development of PSI activity in mature E. acoroides plants but had little effect on PSII activity. To verify the effect of FL on artificially cultured seedlings of E. acoroides, we cultured such seedlings under FL and constant light (CL) indoors. The results showed that the PSI activity of FL-cultured seedlings was lower than that of CL, while the resistance of PSI to eFL was significantly enhanced in FL-cultured seedlings. The increase in PSI resistance of FL-cultured seedlings was attributed to the decrease in active PSII reaction centers, which protected PSI from excessive electrons at the moment when low light turns to high light. This study demonstrated that using FL to cultivate E. acoroides seedlings can improve their adaptability to eFL in the wild, as a possible way to increase the survival rate of transplanted seedlings.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing blue light photobiomodulation for cancer therapy: Evidence from a systematic review.","authors":"Bárbara Evelyn Santos de Lima, Rebeca Barros Nascimento, Ana Paula Mariano Santos Ginez, Maria Stella Moreira, Rebeca Boltes Cecatto, Rodrigo Labat Marcos, Maria Fernanda Setúbal Destro Rodrigues","doi":"10.1111/php.70025","DOIUrl":"https://doi.org/10.1111/php.70025","url":null,"abstract":"<p><p>Cancer is a chronic disease responsible for millions of deaths annually. Its multifaceted profile, with diverse types and anatomical locations, complicates treatment, often limited to surgery, radiotherapy, and chemotherapy. These treatments are frequently associated with increased tumor aggressiveness and recurrence, highlighting the urgent need for new, less invasive therapies. Recent studies suggest that blue light (BL; 450-470 nm) may offer anti-tumor and pro-apoptotic effects, making it a promising alternative for cancer treatment. However, its cellular and molecular mechanisms remain unclear. This qualitative systematic review, conducted in accordance with PRISMA guidelines, analyzed 37 in vitro and in vivo studies published between 2002 and 2024, retrieved from databases including MEDLINE/PubMed, EMBASE, and LILACS, with a focus on the effects of photobiomodulation (PBM) with blue light (450-470 nm) in pre-clinical cancer models. BL demonstrated anti-tumor potential by reducing cell viability, proliferation, migration, and invasion, as well as increasing ROS production and inducing apoptosis. In animal models, BL also inhibited tumor growth, metastasis, and improved survival. Despite the encouraging findings, considerable methodological heterogeneity and insufficient reporting of dosimetric parameters compromise the reproducibility and comparability of results across studies. These findings underscore the therapeutic potential of BL in oncology and highlight the need for standardized protocols to support clinical translation.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thioether-linked iodinated BODIPY-benzimidazole photosensitizer: Efficient light-induced production of singlet oxygen and superoxide radicals and photobiological activity.","authors":"Shawn Swavey, Owen Focht, Devyn Harvey, Emily Dillon, Sherri A McFarland, Ge Shi","doi":"10.1111/php.70030","DOIUrl":"https://doi.org/10.1111/php.70030","url":null,"abstract":"<p><p>A new iodinated BODIPY dye incorporating a thioether- has been synthesized and characterized. The benzimidazole unit was introduced at the meso-pentafluorophenyl position of the BODIPY scaffold via high-yield click chemistry. This substitution does not alter the strong absorption and emission properties of the BODIPY chromophore and provides a versatile platform for the attachment of pharmacologically important molecules. Further functionalization of the BODIPY core with iodine at the 3- and 5-positions yields a derivative capable of generating reactive oxygen species when irradiated with low energy light. Experimental evidence confirms the production of both singlet oxygen and superoxide radicals, indicating this complex is capable of operating by both Type I and Type II photosensitization pathways. This dual capacity could be responsible for its effectiveness as a photosensitizer and contribute to its photobiological activity against human melanoma cells.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen-derived hydroxyproline-containing cyclic dipeptides prevent photoaging-related inflammatory response in UVB-irradiated epidermal keratinocytes.","authors":"Yuki Kumazawa, Kazunori Mizuno, Yuki Taga","doi":"10.1111/php.70026","DOIUrl":"https://doi.org/10.1111/php.70026","url":null,"abstract":"<p><p>Reactive oxygen species (ROS) are major contributors to skin photoaging, which is cumulatively caused by sunlight exposure. We previously developed a unique collagen hydrolysate, named H-GDCH, enriched with hydroxyproline (Hyp)-containing cyclic dipeptides, cyclo(X-Hyp), using ginger protease and subsequent heat treatment. Here, we demonstrated the inhibitory effects of cyclo(X-Hyp) and H-GDCH on ultraviolet B (UVB)-induced photoaging-related inflammatory response in normal human epidermal keratinocytes (NHEK). Cyclo(X-Hyp) significantly decreased intracellular ROS generated by UVB irradiation. The ROS scavenging ability of cyclo(X-Hyp) was superior to that of the corresponding linear dipeptides (X-Hyp) and Pro-containing cyclic dipeptides [cyclo(X-Pro)], respectively. Cyclo(X-Hyp) suppressed ROS-induced activation of inflammatory pathways, including nuclear factor-κB and mitogen-activated protein kinases, and the subsequent increase in matrix metalloproteinase (MMP)-2/MMP-9; moreover, it inhibited the reduction of type IV collagen. ROS production and the downstream events simulated by UVB were also prevented by culturing with H-GDCH. Cyclo(X-Hyp) penetrated human dermatomed skin with high efficiency, reaching 10% after a 24-h incubation. These results indicate that cyclo(X-Hyp) and the cyclo(X-Hyp)-rich collagen hydrolysate, H-GDCH, have the potential as anti-photoaging agents in skin health applications, including cosmetics and functional foods.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic antifungal activity of curcumin-capped TiO₂ nanoparticles: A colorant-based photochemical approach for packaging applications.","authors":"M Paola Sanchez-Castañeda, Leidy T Sanchez, Diana Blach, Cristian C Villa","doi":"10.1111/php.70020","DOIUrl":"https://doi.org/10.1111/php.70020","url":null,"abstract":"<p><p>The present study explores the development, characterization, and antifungal application of curcumin-capped titanium dioxide nanoparticles (cur-TiO₂ Nps) for photodynamic inactivation (PDI) of Aspergillus niger spores, particularly in food packaging contexts. Cur-TiO₂ Nps were synthesized via surface functionalization of anatase-phase TiO₂ with curcumin, and their physicochemical properties were evaluated using XRD, FTIR, UV-Vis spectroscopy, and dynamic light scattering. The structural analyses confirmed the preservation of TiO₂ crystallinity with effective curcumin capping, indicated by characteristic FTIR shifts and a modest increase in the band gap from 3.31 to 3.43 eV. Photoinactivation assays under blue LED irradiation (450 nm) demonstrated that cur-TiO₂ Nps achieved significantly higher antifungal activity (up to 80% inhibition) compared with either curcumin or TiO₂ Nps alone, highlighting a synergistic interaction that enhances reactive oxygen species (ROS) generation. Further evaluation of cur-TiO₂ Nps applied to A. niger-contaminated food packaging surfaces-including polyethylene terephthalate (PET), expanded polystyrene (EPS), and polyvinyl chloride (PVC)-showed material-dependent efficacy. PVC and EPS achieved superior inhibition rates (35% and 27%, respectively), while PET was less effective (~8%), likely due to differences in surface roughness, wettability, and nanoparticle adhesion. These results indicate that cur-TiO₂ Nps are promising candidates for use in light-activated antimicrobial systems, particularly as part of functional food packaging strategies aimed at controlling fungal contamination and extending product shelf life through non-thermal and residue-free methods.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144965106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CdS QDs@SiO₂ immobilized Rh for photo-metal synergistic catalysis of NADH recyclable regeneration.","authors":"Yao Zhang, Shiming Zhang, Li Luo, Sansan Hu, Xujuan Huang, Yongzheng Chen","doi":"10.1111/php.70009","DOIUrl":"10.1111/php.70009","url":null,"abstract":"<p><p>Using visible light to drive NADH regeneration is an economically viable and environmentally sustainable technique. However, it necessitates a metal hydride (MH, [CpRh(bpy)(H₂O)]²⁺) as a synergist, and the high cost of the Rh noble metal significantly impedes the development and application of in-situ NADH regeneration. Therefore, in this study, single-atom Rh was immobilized onto the CdS QDs@SiO₂ combination via a consecutive ball-milling technique in combination with ionic layer adsorption and substitution. Subsequently, an enhanced photo-metal synergistic catalysis system for the recyclable regeneration of NADH was developed. In this composite, the single-atom Rh serves two main functions: It acts as an electrical medium and a metal catalyst, which regulates the activity and selectivity of the regenerated NADH. This study has successfully addressed the key scientific issues regarding the low electron transport rate and the recycling of the Rh noble metal during catalysis. Results confirm that single-atom Rh is successfully immobilized onto the CdS QDs@SiO₂ combination (Rh-CdS@SiO₂) and exhibits a faster electron transport and enhanced selectivity. Under blue light (LED, 420 nm) irradiation, the Rh-CdS@SiO₂ photo-metal catalyst shows a 25-fold increase in recyclable operability and achieves a 68% regeneration yield of NADH in just 4 min. Moreover, (S)-(+)-4-phenyl-2-butanol can be obtained with the regenerated NADH as the coenzyme of P450 enzyme catalysis.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental assessment of photodynamic therapy effects on 4NQO-induced rat tongue carcinogenesis: Clinical, morphological, and immunohistochemical analysis.","authors":"Lauren Frenzel Schuch, Tuany Rafaeli Schmidt, Daniela Campagnol, Vivian Petersen Wagner, Tuane Nerissa Alves Garcez, Alexia Antunes Deluca, Felipe Martins Silvera, Ronell Bologna-Molina, Chris Krebs Danilevicz, Cristiane Helena Squarize, Rogerio Moraes Castilho, Marco Antonio Trevizani Martins, Vanderlei Salvador Bagnato, Cristina Kurachi, Pablo Agustin Vargas, Manoela Domingues Martins","doi":"10.1111/php.70022","DOIUrl":"https://doi.org/10.1111/php.70022","url":null,"abstract":"<p><p>The present study aimed to evaluate the safety of photodynamic therapy (PDT) during oral carcinogenesis using a 4-nitroquinoline 1-oxide (4NQO) rat model, which serves as a surrogate for tobacco-induced oral cancer. Forty male Wistar rats received 4NQO (25 ppm) in drinking water for either 12 or 20 weeks to induce oral lesions. Animals were divided into two groups: Control and PDT. PDT was applied weekly from the beginning of the protocol. A 5-aminolevulinic acid (5-ALA) solution was topically applied to the posterior tongue, followed by laser irradiation guided by fluorescence detection 2 h post-application. Clinical, histopathological, and immunohistochemical (Ki-67) evaluations were performed. Lesions on the posterior dorsum of the tongue manifested in all animals, initially presented as white patches, and over time, progressed to erosive lesions, nodules, and ulcers. Histopathological analysis revealed that epithelial dysplasia predominated at 12 weeks, while squamous cell carcinoma was more frequent at 20 weeks in both groups. No significant differences were observed between the Control and PDT groups concerning clinical appearance or microscopic analysis at either time point. However, Ki-67 expression was significantly lower in the PDT group (p < 0.05), indicating reduced cell proliferation. Cellular proliferation was also downregulated at distant sites, specifically in the anterior tongue, similar to that observed in 4NQO-treated posterior tongue in the PDT group relative to controls at 12 and 20 weeks, suggesting that the antiproliferative effect of PDT extends beyond the irradiated site. Although PDT did not significantly influence lesion progression, it was found to markedly reduce Ki-67 expression levels - even at non-irradiated sites. This observation suggests a potential suppressive effect of PDT on cellular proliferation, which may play a role in modulating tumor growth during the carcinogenesis process.</p>","PeriodicalId":20133,"journal":{"name":"Photochemistry and Photobiology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144848278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}