PharmaNutrition最新文献

{"title":"A single dose of aged garlic extract did not change cerebral oxygenation and blood volume in older adults at cardiovascular risk factors","authors":"Mônica Volino-Souza ,&nbsp;Gustavo Vieira de Oliveira ,&nbsp;Renata Leitão ,&nbsp;Leandro Lara de Carvalho ,&nbsp;Thiago da Silveira Alvares","doi":"10.1016/j.phanu.2023.100345","DOIUrl":"10.1016/j.phanu.2023.100345","url":null,"abstract":"<div><h3>Background</h3><p><span>and Aims: Ageing is associated with impaired endothelial function, which can negatively affect cerebral blood flow (CBF). The present study evaluated whether aged garlic extract (AGE) could improve cerebral </span>oxygenation<span><span> and blood volume due to the possible vasoactive effect. Methods and Results: Fourteen older adults were enrolled in a randomized, double-blind, cross-over study. Urinary </span>thiosulfate<span> and plasma nitrite was drawn, and participants ingested 2.4 g of AGE or placebo. A handgrip exercise protocol started 180 min after supplementation to induce brain activation, and cerebral oxygenation and blood volume were evaluated by near-infrared spectroscopy. After exercise, urinary thiosulfate and plasma nitrite were re-evaluated. AGE supplementation did not change cerebral oxygenation and blood volume. In addition, urinary thiosulfate and plasma nitrite remained unchanged after supplementation. Conclusion: A single dose of AGE (2.4 g) could not increase nitrite levels and cerebral oxygenation and blood volume in older adults at cardiovascular risk factors (NCT04008693).</span></span></p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"25 ","pages":"Article 100345"},"PeriodicalIF":3.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49424408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Royal Jelly consumption on lipid profile: A GRADE-assessed systematic review and dose-response meta-analysis","authors":"Hossein Bahari ,&nbsp;Shaghayegh Taheri ,&nbsp;Mohammad Rashidmayvan ,&nbsp;Zohreh Sajadi Hezaveh ,&nbsp;Sara Ebrahimi Mousavi ,&nbsp;Mahsa Malekahmadi","doi":"10.1016/j.phanu.2023.100351","DOIUrl":"10.1016/j.phanu.2023.100351","url":null,"abstract":"<div><h3>Background</h3><p>Inconsistent evidence exists regarding the impact of Royal Jelly (RJ) on cardio-metabolic risk factors. Hence, this meta-analysis aimed to identify this effect.</p></div><div><h3>Method</h3><p><span>Database search through PubMed/Medline, Web of Science, and SCOPUS was performed until April 2023 to identify eligible trials. Estimated 95% confidence (CI) and the weighted mean difference (WMD) was used for triglyceride (TG), </span>total cholesterol (TC), high-density lipoprotein cholesterol (HDL), and low-density lipoprotein cholesterol (LDL) through the random-effects model.</p></div><div><h3>Results</h3><p>RJ could significantly reduce TC (WMD: −8.75; 95%CI: −15.50, −1.99; <em>P</em> = 0.01; I² = 68.5%; <em>P</em> _{heterogeneity}= 0.21). The protective effect of RJ against serum TC was stronger in unhealthy participants, ≥ 8 weeks of supplement administration, ≥ 3000 mg/d of RJ, and in both sexes. Neither the all-study combination nor the subgroup analysis showed a significant effect of this supplementation on TG, LDL and HDL. Dose-response analysis showed a greater reduction in HDL and a greater elevation in TG, following higher doses of RJ supplementation. Longer duration of intervention also led to lower LDL levels. In terms of the certainty of the evidence, TG, TC and HDL were regarded as moderate and LDL as low quality.</p></div><div><h3>Conclusions</h3><p>RJ can reduce the risk of cardiovascular diseases by reducing the level of TC, and long-term supplementation could improve the level of HDL and LDL.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"25 ","pages":"Article 100351"},"PeriodicalIF":3.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47678236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between dietary polyphenols intake and sleep quality, and mental health in overweight and obese women","authors":"Ashkan Golmohammadi ,&nbsp;Sara Ebrahimi ,&nbsp;Farideh Shiraseb ,&nbsp;Foad Asjodi ,&nbsp;Amir Mehdi Hosseini ,&nbsp;Khadijeh Mirzaei","doi":"10.1016/j.phanu.2023.100338","DOIUrl":"https://doi.org/10.1016/j.phanu.2023.100338","url":null,"abstract":"<div><h3>Background</h3><p>Sleep disorders and mental health issues, including stress, depression, and anxiety, affect many people worldwide, specifically overweight and obese women. This study examined the association between dietary polyphenol intake and mental health, and sleep disorders in overweight and obese women.</p></div><div><h3>Methods</h3><p><span>This cross-sectional study included 278 overweight and obese women from Tehran, Iran. The Pittsburgh Sleep Quality Index (PSQI) and the 21-question version of the </span>Depression Anxiety Stress Scales<span><span> (DASS-21) were used to assess the sleep quality and mental health of participants, respectively. The dietary polyphenol intake was assessed using a semi-quantitative Food Frequency Questionnaire (FFQ) with 147 food items. </span>Anthropometric measurements, body composition analysis, and biochemical parameters were measured according to standard guidelines.</span></p></div><div><h3>Results</h3><p>After controlling for confounders, the higher polyphenol intake was inversely associated with depression (OR=0.744, 95%CI=0.413, 1.338, P-value=0.046) and stress (OR=0.341, 95%CI=0.158, 0.736, P-value=0.006). Furthermore, the DASS score and PSQI score were negatively associated with dietary polyphenols (P-trend=0.063, P-trend=0.074, respectively). No association was found between anxiety and dietary polyphenol intake.</p></div><div><h3>Conclusions</h3><p>Findings showed that dietary polyphenol intake is associated with sleep quality, mental health, and its components, including depression and stress. However, no statistically significant association was found between dietary polyphenol intake and anxiety.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100338"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50179176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary flavonoids: Promising compounds for targeting α-synucleinopathy in Parkinson’s disease","authors":"Marzieh Harati ,&nbsp;Zahra Tayarani-Najaran ,&nbsp;Behjat Javadi","doi":"10.1016/j.phanu.2023.100334","DOIUrl":"10.1016/j.phanu.2023.100334","url":null,"abstract":"<div><h3>Background</h3><p><span>Alpha-synuclein (α-syn) aggregates contribute to neuronal damage and many neurodegenerative disorders (NDs) which are known as α-synucleinopathies. </span>Flavonoids<span><span> are polyphenolic compounds extensively found in various plant-based food such as fruits and vegetables. They have potential benefits in </span>human health, especially in α-synucleinopathy-associated NDs.</span></p></div><div><h3>Objective</h3><p>This review summarizes the latest scientific evidence on the impact of flavonoids on α-synucleinopathy, particularly in Parkinson’s disease</p></div><div><h3>Methods</h3><p>A comprehensive literature search of Web of Science, PubMed, Scopus and Google Scholar was performed to obtain relevant research studies reporting the anti- synucleinopathies effects of dietary flavonoids</p></div><div><h3>Results</h3><p><span><span><span><span>Baicalein, </span>luteolin, </span>quercetin, myricitrin, epigallocatechin-3-gallate and </span>genistein are among the most effective flavonoids against synucleinopathies. The main mechanisms of action contributing to these effects are reducing α-syn production; preventing α-syn aggregation and reducing preformed aggregates; promoting degradation of intracellular α-syn aggregates through autophagy; modulating the activity/expression of antioxidant enzymes; and protecting </span>dopaminergic<span> neurons from oxidative damage and apoptosis</span></p></div><div><h3>Conclusions</h3><p><span>The results of this review highlighted the beneficial effects of flavonoids in the prevention and management of synucleinopathies. However, there is no clinical data that support these effects in humans, which emphasizes the necessity for designing clinical trials to investigate the efficacy, safe dosage, possible side effects and </span>pharmacokinetics characteristics of flavonoids in humans.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100334"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43919862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cranberry juice decreases oxidative stress and improves glucose metabolism in patients with rheumatoid arthritis supplemented with fish oil","authors":"Flávia Troncon Rosa ,&nbsp;Elis Carolina de Souza Fatel ,&nbsp;Daniela Frizon Alfieri ,&nbsp;Tamires Flauzino ,&nbsp;Bruna Miglioranza Scavuzzi ,&nbsp;Marcell Alysson Batisti Lozovoy ,&nbsp;Tatiana Mayumi Veiga Iriyoda ,&nbsp;Andréa Name Colado Simão ,&nbsp;Isaias Dichi","doi":"10.1016/j.phanu.2023.100341","DOIUrl":"10.1016/j.phanu.2023.100341","url":null,"abstract":"<div><h3>Background</h3><p><span>Beneficial effects of fish oil n-3 polyunsaturated fatty acids (PUFAs) have been reported in rheumatoid arthritis (RA), however, high doses of n-3 fatty acids have been associated with increased total and LDL cholesterol and impaired </span>glucose metabolism<span><span><span>. Cranberry products have been reported to improve markers of </span>oxidative stress<span>, inflammation, and the metabolic profile in patients with </span></span>type 2 diabetes mellitus<span> and with metabolic syndrome. We hypothesized that including low-energy cranberry juice on a regimen of fish oil supplementation could improve oxidative stress and attenuate the undesirable effects of fish oil in lipid and glucose metabolism in patients with RA.</span></span></p></div><div><h3>Methods</h3><p>A 90-days randomized controlled trial<span> was conducted. Patients (n = 70) were assigned to one of three groups: control (C); fish oil (FO) received 3 g of fish oil PUFAs supplementation/day; and fish oil and cranberry (FOCR) received 3 g of fish oil PUFAs and 500 mL of cranberry juice/day.</span></p></div><div><h3>Results</h3><p>There was no difference in parameters between FO and the C. FOCR group showed decreased glucose (p = 0.0225), lipid (p = 0.0079), protein (p = 0.0063) oxidation, and Oxidative Stress Index (p = 0.0375) values compared to FO. FOCR reduced glucose values (p = 0.0104), triacylglycerol (p = 0.0065), protein oxidation (p = 0.0042) and Oxidative Stress Index (OSI) (p = 0.0053) compared to the C. Compared to baseline, FO group decreased triacylglycerol (p = 0.0374) and increased glucose (p 0.0001), whereas FOCR group decreased triacylglycerol (p = 0.0398) values.</p></div><div><h3>Conclusion</h3><p>500 mL/day of reduced-calorie cranberry juice in patients with RA using fish oil supplementation decreased lipid, protein oxidation and OSI.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100341"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The efficacy of N-acetylcysteine in improving liver function: A systematic review and meta-analysis of controlled clinical trials","authors":"Mahlagha Nikbaf-Shandiz ,&nbsp;Shaghayegh Adeli ,&nbsp;Amir Hossein Faghfouri ,&nbsp;Fateme Khademi ,&nbsp;Parsa Jamilian ,&nbsp;Meysam Zarezadeh ,&nbsp;Mehrangiz Ebrahimi-Mamaghani","doi":"10.1016/j.phanu.2023.100343","DOIUrl":"10.1016/j.phanu.2023.100343","url":null,"abstract":"<div><h3>Background</h3><p>N-acetylcysteine (NAC) could improve liver function through its antioxidant and anti-inflammatory activities. We conducted a systematic review<span> and meta-analysis of randomized clinical trials to examine whether NAC administration could lead to changes in liver function.</span></p></div><div><h3>Methods</h3><p>The search was done using appropriate keywords in PubMed-Medline, SCOPUS, Embase databases and Google Scholar up to July 2022. Random-effects model was employed to obtain the pooled estimate. Additional analyses including subgroup and sensitivity analyses were carried out.</p></div><div><h3>Results</h3><p><span><span>Eight eligible studies were identified. The results indicated no significant effect of NAC on aspartate aminotransferase (AST), alanine aminotransferase (ALT), </span>alkaline phosphatase (ALP), [(SMD= −0.03 pg/ml; 95% CI: −0.53, 0.47; P = 0.917, I</span>² = 71.3%, P = 0.004), (SMD= −0.22 pg/ml; 95% CI: −0.63, 0.19; P = 0.288, I² = 56.9%, P = 0.040), (SMD= −0.22 pg/ml; 95% CI: −0.67, 0.23; P = 0.343, I²<span> = 52.6%, P = 0.097), respectively], however, it significantly increased albumin (ALB) and decreased bilirubin levels [(SMD= 1.05 pg/ml; 95% CI: 0.41, 1.69; P &lt; 0.001, I</span>² = 87.3%, P &lt; 0.001) and (SMD= −0.34 pg/ml; 95% CI: −0.53, −0.14; P &lt; 0.001, I² = 0.0%, P = 0.447) respectively].</p></div><div><h3>Conclusion</h3><p>Although NAC might have favorable effect on ALB and bilirubin levels, larger trials assessing liver function as primary outcome are required.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100343"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48331828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic-prebiotic therapeutic potential: A new horizon of microbial biotherapy to reduce female reproductive complications","authors":"Kavita Rani ,&nbsp;Gurjeet Kaur ,&nbsp;Syed Azmal Ali","doi":"10.1016/j.phanu.2023.100342","DOIUrl":"10.1016/j.phanu.2023.100342","url":null,"abstract":"<div><h3>Background</h3><p><span>Probiotics and associated </span>prebiotics<span> have emerged as a novel method for treating health issues in the last two decades. It has been shown that they can regulate the gut microbiota<span><span> through different molecular mechanisms. Inappropriate lifestyle choices activate the hypothalamic-pituitary-adrenal axis, resulting in immense stress. This leads to an increased incidence of patients with metabolic syndrome<span> and an altered gut microbiota further lead to gastrointestinal dysbiosis. Microbial dysbiosis can disrupt the control and synthesis of </span></span>reproductive hormones, thus leading to infertility.</span></span></p></div><div><h3>Methods</h3><p>In this review, we gathered information from recent studies that show how probiotics and prebiotics could help support the treatment<span><span> of reproductive disorders and hormone imbalances. We discussed the biochemical effects and proposed mechanisms of action of probiotics and prebiotics that may help with infertility in humans, as well as how these </span>functional foods could be used to treat reproductive disorders.</span></p></div><div><h3>Results</h3><p>Probiotics could restore fertility because their metabolites trigger the release of signaling molecules that control hormone production in intestinal epithelial cells and other organs. The review provides a collection of evidence that the use of probiotics and prebiotics could be a new horizon of microbial biotherapy to reduce female reproductive complications.</p></div><div><h3>Conclusions</h3><p>Probiotics and prebiotics may offer a new approach to treat reproductive disorders and infertility by regulating gut microbiota and restoring the control and synthesis of reproductive hormones. The potential of using these functional foods as a microbial biotherapy to alleviate female reproductive complications warrants further investigation.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100342"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48753708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of grape products on metabolic syndrome risk factors: A systematic review and meta-analysis","authors":"Adriele Debortoli da Silva ,&nbsp;Natália Oliveira Izidoro ,&nbsp;Leandro Roberto de Macedo ,&nbsp;Ione Maria de Matos ,&nbsp;Maísa Silva","doi":"10.1016/j.phanu.2023.100339","DOIUrl":"10.1016/j.phanu.2023.100339","url":null,"abstract":"<div><h3>Background</h3><p>Grape supplementation has been suggested to contribute to improving parameters related to metabolic syndrome (MetS); however, the scientific literature in this field is controversial and inconclusive.</p></div><div><h3>Objectives</h3><p><span>We undertook a systematic review<span> and meta-analysis of randomized controlled clinical trials on the effect of grape product on metabolic syndrome components, including blood pressure, </span></span>anthropometric<span> measurements, and glycemic and lipid profiles.</span></p></div><div><h3>Methods</h3><p>The online databases of PubMed, Scopus<span>, Web of Science, and SciELO were systematically searched for relevant publications until December 2021. The review has been registered at PROSPERO (CRD42021287813). To compare the effects of grape product (seed extract, powder, extract, fruit, pomace, juice) with placebo, the mean differences with 95% confidence intervals (CI) were pooled based on the random-effects model. Subgroup analyses were performed to identify the source of heterogeneity, and assessment of study quality was conducted using the GRADE approach.</span></p></div><div><h3>Results</h3><p>In total, 27 randomized controlled trials<span><span><span> (RCTs) were included in this systematic review, and 21 trials were selected for meta-analysis. Meta-analyses of the data showed that grape products had a significant effect on lipid levels, decreasing total and </span>low density lipoprotein cholesterol<span> and triacylglycerol levels. Grape supplementation also reduced systolic and diastolic </span></span>arterial pressure<span>. In addition, grape supplementation increased glucose serum and insulin levels. We found no significant differences in anthropometric measurements.</span></span></p></div><div><h3>Conclusion</h3><p><span>grape products may be beneficial for individuals with parameters related to MetS because they may have hypotensive and hypolipidemic properties</span><strong>.</strong></p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100339"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47462810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics in pregnancy: Inequities in knowledge exchange, attitudes, and use of probiotics in a socio-demographically diverse, cross-sectional survey sample of pregnant Canadians","authors":"L. McKerracher ,&nbsp;T. Moffat ,&nbsp;M.E. Barker ,&nbsp;B. Murray-Davis ,&nbsp;K.M. Kennedy ,&nbsp;C.J. Bellissimo ,&nbsp;E. Yeo ,&nbsp;D. Høtoft ,&nbsp;L. Zalot ,&nbsp;V. Parlette ,&nbsp;S.A. Atkinson ,&nbsp;D.M. Sloboda","doi":"10.1016/j.phanu.2023.100344","DOIUrl":"10.1016/j.phanu.2023.100344","url":null,"abstract":"<div><h3>Background</h3><p>Pregnancy interventions, potentially including consumption of nutraceuticals like probiotics, represent possible avenues for preventing non-communicable diseases. However, evidence syntheses indicate that probiotic interventions, while effective in managing some pregnancy complications (e.g., gestational diabetes), do not confer health benefits to uncomplicated pregnancies. Messaging around probiotics in pregnancy is mixed, such that people with low-risk pregnancies may nevertheless feel pressure to spend limited resources on (costly) probiotics. To tailor knowledge exchange and support safe, equitable access to pregnancy probiotics when their prescription may be warranted, we need to understand who takes probiotics during pregnancy and under what conditions.</p></div><div><h3>Methods</h3><p>We used chi-square and logistic regression analyses of anonymous, cross-sectional survey data from 341 pregnant Canadians of diverse socio-demographic backgrounds to assess which respondents, by socio-demographic characteristics and pre-pregnancy/pregnancy health indicators, were relatively likely to: perceive probiotics as beneficial to pregnancy health and/or report taking probiotics during pregnancy.</p></div><div><h3>Results</h3><p>Forty-seven percent of respondents perceived probiotics as beneficial to pregnancy health; 51 % reported consuming them. Probiotic attitudes and consumption were socio-demographically-patterned: higher-income, post-secondary-educated respondents disproportionately perceived probiotics as healthy and consumed them. There was no evidence of variation in probiotics attitudes or use by pregnancy health indicators.</p></div><div><h3>Conclusion</h3><p>Socio-economic factors may be more important determinants of pregnancy probiotic use in this sample than indications for pregnancy complications. Clear guidelines on pregnancy probiotics that reflect current evidence are needed. Equitable access to probiotics should be facilitated for pregnant people likely to benefit from interventions (i.e., those with certain complications), supporting long-term health equity.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100344"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46062522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soy isoflavone daidzein protects Neuro2a cells from NO stress via activation of AMPK-PGC1α pathway followed by mitochondrial enhancement","authors":"Shogo Ito ,&nbsp;Hiroko Sasaki ,&nbsp;Takahiro Gotow ,&nbsp;Isao Suetake ,&nbsp;Kaoru Nagai","doi":"10.1016/j.phanu.2023.100337","DOIUrl":"10.1016/j.phanu.2023.100337","url":null,"abstract":"<div><h3>Background</h3><p>Soy<span><span><span> isoflavone </span>daidzein protects cells from </span>oxidative stress<span>. Oxidative stress causes several neurodegenerative diseases<span> via damaging mitochondria and inducing cell death.</span></span></span></p></div><div><h3>Methods</h3><p><span>In this study, we analyzed protective mechanisms of daidzein on nitric oxide (NO) induced neuronal Neuro2a cell death, and quantitated mitochondrial amount, that was analyzed by cytometric analysis and </span>western blots of mitochondrial molecules.</p></div><div><h3>Results</h3><p><span><span>Daidzein reduced cell death against NO stress and increased mitochondrial amount even in the presence of NO. Regarding the mechanism of mitochondrial increase, daidzein increased the expression of mitochondrial biogenesis regulator Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) and induced nuclear translocation of PGC1α. Following this, we analyzed if the reduction of PGC1α reduces the protective function of daidzein against oxidative stress. Knockdown of PGC1α abolished the cell-protective function. It strongly suggests that PGC1α activation followed by the mitochondrial increase is critical for the </span>cell protection<span>. Next, we focused on 5′-adenosine monophosphate-activated protein kinase (AMPK) for clarifying how daidzein activates PGC1α. Daidzein increased the expression and phosphorylation of AMPK, indicating that daidzein stimulates AMPK activation. </span></span>AMPK inhibitor<span> dorsomorphin abolished the cell protection and suppressed the nuclear translocation of PGC1α. Additionally, dorsomorphin prevented the increase of mitochondria by daidzein.</span></p></div><div><h3>Conclusion</h3><p>Our study strongly suggests that daidzein protects Neuro2a cells via AMPK activation followed by PGC1α activation and mitochondrial biogenesis.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100337"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46429382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}