PharmaNutrition最新文献_第8页

From antiepileptic therapy to promising adjuvant in medical oncology: A historical view of the ketogenic diet 从抗癫痫治疗到医学肿瘤学中有前景的佐剂：生酮饮食的历史观点

IF 3.2

PharmaNutrition Pub Date : 2023-06-01 DOI: 10.1016/j.phanu.2023.100340

Doaa N. Al-Jada , Hamed R. Takruri , Wamidh H. Talib

{"title":"From antiepileptic therapy to promising adjuvant in medical oncology: A historical view of the ketogenic diet","authors":"Doaa N. Al-Jada , Hamed R. Takruri , Wamidh H. Talib","doi":"10.1016/j.phanu.2023.100340","DOIUrl":"10.1016/j.phanu.2023.100340","url":null,"abstract":"<div><h3>Background</h3><p>Despite recent advances that have been made in cancer treatment, we still lack complementary approaches to significantly improve the effectiveness of current anti-cancer therapies. The high-fat, low-carbohydrate ketogenic diet<span><span> can cause metabolic changes in the tumor microenvironment, thus targeting cancer metabolism, making it an attractive candidate for </span>adjuvant cancer therapy<span><span>. This review is an attempt to explore the origin and trace the historical use of the ketogenic diet as a metabolic therapy in </span>medical oncology.</span></span></p></div><div><h3>Methods</h3><p>A detailed literature review was conducted through specialized databases. Only those articles pertinent to the specific needs of the review were included. Relevant references in those articles were also followed.</p></div><div><h3>Results</h3><p>There is a sound biological rationale for the anti-tumor force of the ketogenic diet primarily manifested through its anti-Warburg effect. It is no less true that Otto Warburg’s experiments have based the ketogenic diet in cancer research on solid footing. However, precisely tracing the initial, original anti-tumor use of the ketogenic diet in medical oncology literature has indeed proved to be a hard needle to thread. The currently available data are considered promising in the pre-clinical rodent models. Yet, the number of rigorous human trials is small and suffers from methodological limitations.</p></div><div><h3>Conclusions</h3><p>Further research, both pre-clinical and clinical, is needed to accurately define the anti-tumor effect of the ketogenic diet and to overcome the current methodological limitations that hinder us from deriving a sound judgment about the use of the ketogenic diet in clinical practice.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"24 ","pages":"Article 100340"},"PeriodicalIF":3.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42081312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of dietary supplementation with coenzyme Q10 on periodontitis: A systematic review and meta-analysis 膳食补充辅酶Q10对牙周炎的影响:系统回顾和荟萃分析

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2022.100328

Haniyeh Golafrouz , Farzad Shidfar , Arezoo Piran , Zohreh Sajadi Hezaveh

{"title":"Impact of dietary supplementation with coenzyme Q10 on periodontitis: A systematic review and meta-analysis","authors":"Haniyeh Golafrouz , Farzad Shidfar , Arezoo Piran , Zohreh Sajadi Hezaveh","doi":"10.1016/j.phanu.2022.100328","DOIUrl":"10.1016/j.phanu.2022.100328","url":null,"abstract":"<div><p><span><span><span><span>To explore the impact of CoQ10 supplementation on </span>periodontitis, a </span>systematic review<span> of controlled trials (PROSPERO: CRD42021274876) was performed from 1970 to November 2022 through Web of Science (ISI), Google Scholar, Embase<span>, CENTRAL, PubMed/MEDLINE, Scopus<span>, and ProQuest. A random-effect model was used considering clinical attachment loss (CAL), </span></span></span></span>plaque index (PI), gingival index (GI), bleeding index (BI), and probing depth (PD). Of 97 records, eight trials were included. CoQ10 supplementation significantly reduce CAL (WMD, –0.66 (–0.81 to –0.50), P < 0.0001; I</span><sup>2</sup><span> = 93.4 %) and GI (WMD, –0.20 (–0.40–0.00), P = 0.05; I</span><sup>2</sup> = 98.5 %), but it could not affect PI (WMD, –0.07 (–0.16–0.02), P = 0.11, I<sup>2</sup> = 91.2 %), PD (WMD, –0.28 (–0.67–0.12), P = 0.17; I<sup>2</sup> = 98.4 %), and BI (WMD, –0.58 (–1.40–0.24), P = 0.17; I<sup>2</sup> = 99.5 %) significantly. CoQ10 had a stronger reduction effect on PI and GI in individuals ≥ 40 years old and in doses of ≤ 60 mg CoQ10. Longer duration of CoQ10 supplementation was also more effective in reducing PD. CAL reduced greater when CoQ10 was administered for longer than 12 weeks, in a dose of > 60 mg, and in younger individuals. CoQ10 supplementation in periodontitis may have beneficial effect on GI and CAL. Further investigations in this regard are needed to draw conclusive results.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100328"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44812153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medium-chain triglycerides reduce glomerulosclerosis and induce expression of redox genes in NSY mice with diabetic nephropathy 中链甘油三酯减少糖尿病肾病NSY小鼠肾小球硬化并诱导氧化还原基因表达

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2022.100325

Mai Hagiwara , Shiori Ishiyama , Kazuki Mochizuki

{"title":"Medium-chain triglycerides reduce glomerulosclerosis and induce expression of redox genes in NSY mice with diabetic nephropathy","authors":"Mai Hagiwara , Shiori Ishiyama , Kazuki Mochizuki","doi":"10.1016/j.phanu.2022.100325","DOIUrl":"10.1016/j.phanu.2022.100325","url":null,"abstract":"<div><h3>Background</h3><p>Nephropathy<span><span> is a type of complication of type 2 diabetes, and </span>medium chain triglycerides (MCTs) potentially improve metabolic abnormalities. This study examined whether MCT intake inhibits nephropathy development in a type 2 diabetic mouse model.</span></p></div><div><h3>Methods</h3><p><span><span>Six-week-old control non-diabetic ICR mice and type 2 diabetic Nagoya Shibata Yasuda (NSY) mice were fed a diet enriched with long-chain saturated/mono-unsaturated </span>triglycerides, including palmitic-, stearic-, and </span>oleic acids<span><span><span> (C16/C18-rich group). NSY mice were fed a diet containing medium-chain triglycerides with either caprylic acid (C8-rich group) or </span>capric acid<span> (C10-rich group). Feeding was performed for 20 weeks. Plasma biomarker levels were measured using ELISA or </span></span>enzymatic methods<span>. Kidney pathology was evaluated using periodic acid–Schiff staining, and the expression of redox genes and proteins were determined using qRT-PCR and western blotting.</span></span></p></div><div><h3>Results</h3><p><span>The C16/C18-rich group of NSY mice exhibited expansion of the mesangial region in the kidney. Plasma cystatin C and creatinine concentrations were not different between ICR mice and C16/C18-rich group of NSY mice and between C16/C18-rich and C8- or C10-rich groups of NSY mice. The C8- and C10-rich groups had suppressed mesangial region than the C16/C18-rich groups. The mRNA expression of </span><em>Sod1</em>, <em>Prdx1</em>, and <em>Gpx2</em>, and SOD1 protein levels were higher in the C8- and/or C10-rich groups than in the C16/C18-rich group of NSY mice.</p></div><div><h3>Conclusions</h3><p><span>MCT intake in NSY mice at the stage without increased markers indicating glomerular filtration decrease, reduces </span>glomerulosclerosis, with induction of redox gene expression, in the kidney.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100325"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43558232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nutraceutical and dietary resources for breast cancer prevention – Highlighting strategies for suppressing breast aromatase expression 癌症预防的营养和饮食资源——抑制乳腺芳香化酶表达的重点策略

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2022.100327

Simon Bernard Iloki Assanga , Lidianys María Lewis Luján , Mark F. McCarty , James J. DiNicolantonio

{"title":"Nutraceutical and dietary resources for breast cancer prevention – Highlighting strategies for suppressing breast aromatase expression","authors":"Simon Bernard Iloki Assanga , Lidianys María Lewis Luján , Mark F. McCarty , James J. DiNicolantonio","doi":"10.1016/j.phanu.2022.100327","DOIUrl":"10.1016/j.phanu.2022.100327","url":null,"abstract":"<div><h3>Background</h3><p><span>Visceral obesity and the metabolic syndrome<span><span><span> that often accompanies it are robust and well-established risk factors for postmenopausal breast cancer. This increased risk is thought to be mediated, in large part, by increased aromatase<span> expression in the breast. stromal adipocytes and in </span></span>mammary epithelium; this results in a high local concentration of estrogen capable of supporting the cancerous transformation of estrogen receptor-positive (ER+) epithelial cells. Aromatase, a member of the </span>cytochrome 450 family, is expressed in the </span></span>endoplasmic reticulum<span><span>. After menopause, it converts androgens produced within the adrenal cortex (androstenedione and testosterone) into estrogens (estrone and </span>estradiol<span>, respectively. Therefore, the main objective of this narrative article is to review dietary and nutraceutical remedies for the prevention of menopause. Breast cancer: leading strategies to suppress breast aromatase</span></span></p></div><div><h3>Methods</h3><p>The research articles to carry out this work were focused based on many searches and reviews in the following databases: Google Scholar, MDPI, PubMed, ScienceDirect and using the following keywords and combined synonyms: (\"nutraceuticals\" or \"supplements\" or \"aromatase inhibitors\" or \"aromatase\" or \"antioxidants”) AND (“breast cancer” or “vascular function” or “inflammation”). The keywords were also searched in the references of the original articles included in this study</p></div><div><h3>Conclusion</h3><p>A number of nutraceutical, dietary, and lifestyle measures hold promise for breast cancer prevention and may be considered practical options by women seeking to minimize their risk. for this cancer, particularly as these measures appear to have few downsides and are likely to work in other ways to promote health.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100327"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47157569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The impact of PUFA-enriched yogurt consumption on cardiovascular risk markers: A review 富含pufa的酸奶对心血管危险指标的影响:综述

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2023.100330

Laura-Natalia Cortés , Ruby-Alejandra Villamil , Lilia-Yadira Cortés

{"title":"The impact of PUFA-enriched yogurt consumption on cardiovascular risk markers: A review","authors":"Laura-Natalia Cortés , Ruby-Alejandra Villamil , Lilia-Yadira Cortés","doi":"10.1016/j.phanu.2023.100330","DOIUrl":"10.1016/j.phanu.2023.100330","url":null,"abstract":"<div><h3>Background</h3><p>Yogurt, as one of the mass consumption dairy products worldwide, has been made a carrier of bioactive compounds, such as polyunsaturated fatty acids (PUFAs). Since cardiovascular disease (CVD) remains the leading cause of mortality worldwide dietary trends, including PUFAs-enriched yogurts, can be used to address this public health issue and contribute to a better healthcare.</p></div><div><h3>Objective</h3><p>This work describes the effects of the consumption of yogurt enriched with PUFAs on cardiovascular biomarkers.</p></div><div><h3>Methods</h3><p>A systematic review following the PRISMA methodology was done by gathering relevant published randomized controlled trials in adult population from 2000 to 2021 through Cochrane, PubMed, and Scopus. To control bias, we examined Cochrane Handbook recommendations.</p></div><div><h3>Results</h3><p>In the works reviewed, PUFAs-functionalized yogurt led to a reduction of triglycerides (TG) in plasma and an increase in the expression of the peroxisome proliferator- activated receptor gamma (PPARγ). PUFA supplementation in yogurt did not alter body weight or low-density lipoprotein (LDL) plasma levels. A dose of 200 g/d of PUFAs-enriched yogurt (PUFAs content range between 0.64% and 16.02% w/w) was identified. Among the tested PUFA sources (plant, seaweed, and animal), with which yogurt was supplemented, fish oil resulted in the best cardiovascular health benefits across interventions.</p></div><div><h3>Conclusions</h3><p>Although, all of the reviewed interventions with PUFAs- functionalized yogurt revealed variable effects on cardiovascular health, these still represent a limited number, and more studies are necessary to draw stronger conclusions and actionable information.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100330"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43317671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Predictive factors for allergy at 4–6 years of age based on machine learning: A pilot study 基于机器学习的4-6岁过敏预测因素:一项试点研究

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2022.100326

Kim Kamphorst , Alejandro Lopez-Rincon , Arine M. Vlieger , Johan Garssen , Esther van ’t Riet , Ruurd M. van Elburg

{"title":"Predictive factors for allergy at 4–6 years of age based on machine learning: A pilot study","authors":"Kim Kamphorst , Alejandro Lopez-Rincon , Arine M. Vlieger , Johan Garssen , Esther van ’t Riet , Ruurd M. van Elburg","doi":"10.1016/j.phanu.2022.100326","DOIUrl":"10.1016/j.phanu.2022.100326","url":null,"abstract":"<div><h3>Background</h3><p>In Europe, allergic diseases are the most common chronic childhood illnesses and the result of a complex interplay between genetics and environmental factors. A new approach for analyzing this complex data is to employ machine learning (ML) algorithms. Therefore, the aim of this pilot study was to find predictors for the presence of parental-reported allergy at 4–6 years of age by using feature selection in ML.</p></div><div><h3>Methods</h3><p>A recursive ensemble feature selection (REFS) was used, with a 20% step reduction and with eight different classifiers in the ensemble, and resampling given the class unbalance. Thereafter, the Receiver Operating Characteristic Curves for five different classifiers, not included in the original ensemble feature selection technique, were calculated.</p></div><div><h3>Results</h3><p>In total, 130 children (14 with and 116 without parental-reported allergy) and 248 features were included in the ML analyses. The REFS algorithm showed a result of 20 features and particularly, the Multi-layer Perceptron Classifier had an area under the curve (AUC) of 0.86 (SD 0.08). The features predictive for allergy were: tobacco exposure during pregnancy, atopic parents, gestational age, days of: diarrhea, cough, rash, and fever during first year of life, ever being exposed to antibiotics, Resistin, IL-27, MMP9, CXCL8, CCL13, Vimentin, IL-4, CCL22, GAL1, IL-6, LIGHT, and GMCSF.</p></div><div><h3>Conclusions</h3><p>This ML model shows that a combination of environmental exposures and cytokines can predict later allergy with an AUC of 0.86 despite the small sample size. In the future, our ML model still needs to be externally validated.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100326"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47255395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral bioavailability and neuroprotective effect of a novel food-grade formulation of fisetin using fenugreek-galactomannan hydrogel scaffolds 采用胡芦巴-半乳甘露聚糖水凝胶支架的新型食品级非瑟酮制剂的口服生物利用度和神经保护作用

IF 3.2

PharmaNutrition Pub Date : 2023-03-01 DOI: 10.1016/j.phanu.2023.100329

K. Athira , S. Syam Das , Andrew Swick , I.M. Krishnakumar , A. Abdul Vahab

{"title":"Oral bioavailability and neuroprotective effect of a novel food-grade formulation of fisetin using fenugreek-galactomannan hydrogel scaffolds","authors":"K. Athira , S. Syam Das , Andrew Swick , I.M. Krishnakumar , A. Abdul Vahab","doi":"10.1016/j.phanu.2023.100329","DOIUrl":"10.1016/j.phanu.2023.100329","url":null,"abstract":"<div><h3>Background</h3><p>Despite the beneficial pharmacological effects, poor oral bioavailability limits the nutritional efficacy of fisetin<span>, a dietary flavonoid. To this end, herein we report the bioavailability and efficacy of an innovative formulation of fisetin using a fenugreek-galactomannan hydrogel scaffold (FF-20).</span></p></div><div><h3>Methods</h3><p>In the first phase of the study, female Wistar rats<span> (n = 42) were randomly divided into two groups (n = 21/group) and orally administered with either unformulated (UF) or FF-20 (50 mg/kg b. wt.) and plasma concentration of fisetin was estimated by UPLC-ESI-MS/MS. In the second phase, the relative influence of FF-20 on alcohol-induced neurotoxicity was followed on animals (n = 24) randomized into four groups, Group I – vehicle control, Group II – ethanol treated, Group III – ethanol+ UF, and Group IV – ethanol + FF-20 and treated at 50 mg/kg b. wt. per day for 14 days.</span></p></div><div><h3>Results</h3><p><span>Area under plasma concentration verses time curve showed 9.83-fold enhancement in bioavailability for FF-20, with significantly enhanced pharmacokinetic parameters (*** </span><em>P</em> < 0.001). Behavior studies revealed significant improvement in reference memory errors, working memory errors, and anxiety among fisetin-treated animals and the improvement was significant in FF-20, compared to UF (* <em>P</em><span><span><span> < 0.05). Neurotransmitters<span> and gene expressions of NMDAR, MAO A&B, and KLF-11 were altered by alcohol </span></span>treatment; but were restored/improved in FF-20 group. </span>Histopathology<span> of brain tissues also indicated the reversal of alcohol-induced necrosis and tissue damage by FF-20.</span></span></p></div><div><h3>Conclusion</h3><p>FF-20 enhanced the oral bioavailability and significantly alleviated alcohol-induced neurotoxicity in experimental rats.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"23 ","pages":"Article 100329"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44203958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of dietary habits and use of nutritional supplements in COVID-19: A cross-sectional study 评估COVID-19患者的饮食习惯和营养补充剂的使用:一项横断面研究

IF 3.2

PharmaNutrition Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100309

Cagla Ayer , Adviye Gulcin Sagdicoglu Celep

{"title":"Assessment of dietary habits and use of nutritional supplements in COVID-19: A cross-sectional study","authors":"Cagla Ayer , Adviye Gulcin Sagdicoglu Celep","doi":"10.1016/j.phanu.2022.100309","DOIUrl":"10.1016/j.phanu.2022.100309","url":null,"abstract":"<div><h3>Objective</h3><p>Determine nutritional status and use of food supplements during COVID-19.</p></div><div><h3>Methods</h3><p>Sample of individuals aged 18–65 participated in study voluntarily. Patients with COVID-19 or individuals with contact were not included. Questionnaire form was prepared based on literature on food supplements and included questions adapted to COVID-19 pandemic.</p></div><div><h3>Results</h3><p>488 participants completed the study. Participants’ mean age was 26.28 ± 7.64, %82.0 were female, 88.3% were high educated. During COVID-19, 33.6% exercise less than 150 min/day, 55.1% evaluate their eating habits as good/very good, and number of meals did not change compared to before pandemic (41.8%). 34.2% of participants consume 5–7 cups of water daily during pandemic. Consumption of pastry (54.7%) and green leafy vegetables (49.6%) increased. 78.7% of participants did not use any nutritional supplement, and 51.1% stated that they did not need nutritional supplements. Multivitamin and mineral (16.5%), vitamin D (15.3%), and vitamin C (11.4%) are used the most, and 56.7% have been using these products for 1–3 months. Use of nutritional supplements was recommended mostly by doctors (39.4%). 43.1% stated that they used these products to maintain good health, 21.9% because they felt tired, 13.8% because they did not have adequate and balanced nutrition. 51.0% of those using nutritional supplements benefited, 30.8% had no effect.</p></div><div><h3>Conclusions</h3><p>Participants did not make significant changes in their eating habits compared to before pandemic, the use of nutritional supplements increased to maintain good health.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"22 ","pages":"Article 100309"},"PeriodicalIF":3.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9423873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10410851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Functional differences between primary monocyte-derived and THP-1 macrophages and their response to LCPUFAs 原代单核细胞源性和THP-1巨噬细胞的功能差异及其对LCPUFAs的反应

IF 3.2

PharmaNutrition Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100322

Tamara Hoppenbrouwers , Shanna Bastiaan-Net , Johan Garssen , Nicoletta Pellegrini , Linette E.M. Willemsen , Harry J. Wichers

{"title":"Functional differences between primary monocyte-derived and THP-1 macrophages and their response to LCPUFAs","authors":"Tamara Hoppenbrouwers , Shanna Bastiaan-Net , Johan Garssen , Nicoletta Pellegrini , Linette E.M. Willemsen , Harry J. Wichers","doi":"10.1016/j.phanu.2022.100322","DOIUrl":"10.1016/j.phanu.2022.100322","url":null,"abstract":"<div><h3>Background</h3><p>In immune cell models, macrophages are one of the most frequently used cell types. THP-1 cells are often used as model to study macrophage function, however they may act differently from primary human monocyte derived macrophages (MDMs).</p></div><div><h3>Methods</h3><p>In this study, we investigated the intrinsic baseline differences between the human macrophage cell line THP-1 and human primary MDMs. Additionally, we studied the difference in response to treatment with long-chain polyunsaturated fatty acids (LCPUFAs): well-described immunomodulators.</p></div><div><h3>Results</h3><p>Although the amount of cells that phagocytose were similar between the cell types, primary MDMs consumed significantly more <em>E. coli</em> bioparticles compared to THP-1 macrophages. In M1 macrophages, IL-12 secretion was almost fifty times higher by primary MDMs compared to THP-1 macrophages, thereby increasing the IL-12/IL-10 ratio. Despite this, the IL-12 secretion by THP-1 M1 macrophages was higher that the secretion of IL-10, thereby showing that it is still a suitable M1 type. Cytokine profiles differed between primary MDMs and THP-1 M1 and M2 macrophages. In response to LCPUFAs, primary M1 MDMs and THP-1 M1 macrophages were alike. Interestingly, primary M2 MDMs secreted less IL-10 and CCL22 when treated with LCPUFAs, whereas THP-1 M2 macrophages secreted more IL-10 when treated with LCPUFAs and showed no difference in CCL22 secretion.</p></div><div><h3>Conclusions</h3><p>In conclusion, in an M1 setting, both THP-1 and primary MDMs are suitable models. However, when interested in M2 models, the model choice highly depends on the research question.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"22 ","pages":"Article 100322"},"PeriodicalIF":3.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213434422000354/pdfft?md5=9de3d43e964a508ac81a595e47db114e&pid=1-s2.0-S2213434422000354-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43376171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vitamin D status can affect COVID-19 outcomes also in pediatric population 维生素D状况也会影响儿科人群的COVID-19结局

IF 3.2

PharmaNutrition Pub Date : 2022-12-01 DOI: 10.1016/j.phanu.2022.100319

Michele Miraglia del Giudice , Cristiana Indolfi , Giulio Dinardo , Fabio Decimo , Alberto Decimo , Angela Klain

引用次数: 7