Physiological and Molecular Plant Pathology最新文献

{"title":"Recent advances in phosphorus nano-fertilizers: Impacts on crop productivity and soil sustainability","authors":"Vivek Kumar Dhiman ,&nbsp;Vinay Kumar Dhiman ,&nbsp;Garima Rana ,&nbsp;Rakesh Kumar ,&nbsp;Devendra Singh ,&nbsp;Ankush Chauhan ,&nbsp;Majid Jabir ,&nbsp;Suresh Ghotekar","doi":"10.1016/j.pmpp.2025.102885","DOIUrl":"10.1016/j.pmpp.2025.102885","url":null,"abstract":"<div><div>Phosphorus nano-fertilizers (P-nFs) can play a key role in the future of sustainable food production. Controlled release properties of these fertilizers reduce the leaching and eutrophication problems, reducing the fertilizer application rate, thereby ensuring nutrient use efficiency and reducing the input cost substantially. When traditional phosphorus fertilizers are applied to the soil, they get fixed in the soil, which results in limited availability to the plants. This leads to excessive fertilizer application. The aid of nanotechnology could overcome this problem. Nano phosphorus fertilizers are not fixed in the soil, and their release rate can be controlled, making them available for a longer duration in the growing season. This makes the nano phosphorus fertilizers environment-friendly and more efficient than traditional fertilizers. This paper provides a detailed review of the recent studies of nano phosphorus fertilizers in agriculture, and specific basic concerns and existing gaps in research need to be addressed and resolved. Despite these promising benefits, challenges such as synthesis scalability, environmental safety, and regulatory gaps continue to hinder large-scale adoption of P-nFs.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102885"},"PeriodicalIF":3.3,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144867418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan nanoparticles loaded with jasmonic acid induce plants’ resistance against Botrytis cinerea","authors":"Theoni Margaritopoulou ,&nbsp;Anastasios Sakellariou ,&nbsp;Georgios Sofianos ,&nbsp;Maria-Frantzeska Triviza ,&nbsp;Despoina-Maria Stika ,&nbsp;Despoina Tsiriva ,&nbsp;Georgios Karaoglanidis ,&nbsp;Emilia Markellou","doi":"10.1016/j.pmpp.2025.102887","DOIUrl":"10.1016/j.pmpp.2025.102887","url":null,"abstract":"<div><div>Today, there is a growing need for environmentally friendly and sustainable compounds to manage plant diseases. The application of plant defense inducers is a promising eco-friendly strategy to combat fungal pathogens. Chitosan nanoparticles are polymeric, bio-based carriers of active compounds that are widely used in plant protection. In this study, we demonstrate that chitosan nanoparticles loaded with jasmonic acid (JA-CNPs) effectively induce the jasmonic acid defense pathway and reduce the severity of infection caused by the necrotrophic fungus <em>Botrytis cinerea</em> (strain B05.10) on <em>Αrabidopsis thaliana</em> and tomato leaves by 51 % and 45 %, respectively. To assess defense responses, a β-glucuronidase staining (GUS-staining) experiment was conducted using the <em>PDF1.2:GUS A. thaliana</em> transgenic line, demonstrating that JA-CNPs effectively induce the jasmonic acid (JA) pathway at a low concentration of 5 ppm. This effect remained evident even after artificial inoculation with <em>B. cinerea</em> conidia. Confocal microscopy revealed reduction of hyphal development of the pathogen in Arabidopsis leaves treated with 5 ppm JA-CNPs, when compared to control conditions. <em>In vitro</em> bioassays revealed that JA-CNPs did not directly affect <em>B. cinerea</em> conidial germination, reinforcing their role in defense induction in <em>A. thaliana</em>. Furthermore, transcriptional analysis of <em>COI</em>, <em>ASK1</em>, <em>AXR1</em>, and <em>JAH3</em> in <em>A. thaliana</em>, as well as <em>AOX</em>, <em>PME1</em>, <em>CHS1</em>, and <em>BOA</em>3 in <em>B. cinerea</em>, revealed that JA-CNPs application influences key components of the plant's JA pathway and challenges the virulence mechanisms of the fungus, respectively. Overall, these findings highlight the potential of JA-CNPs as a promising tool for the control of <em>B. cinerea</em>, contributing to minimization of chemical fungicides in crop protection.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102887"},"PeriodicalIF":3.3,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical role for the LRR receptor kinase PcLRR-RK2 in the physiology and pathogenesis of Phytophthora capsici","authors":"Asma Safdar ,&nbsp;Mustansar Mubeen ,&nbsp;Daolong Dou ,&nbsp;Yasir Iftikhar ,&nbsp;Amin A. Al-Doaiss ,&nbsp;Ahmed Ezzat Ahmed ,&nbsp;Manoj Kumar Solanki","doi":"10.1016/j.pmpp.2025.102884","DOIUrl":"10.1016/j.pmpp.2025.102884","url":null,"abstract":"<div><div>Plants contain many proteins known as Leucine-rich repeat receptor-like kinases (LRR-RLKs). These proteins aided in the transmission of signals within cells, which are important for development, growth, and defense against biotic and abiotic threats. Although oomycetes also include LRR-RLKs, not much is known about their function in these organisms. In this research, <em>PcLRR-RK2</em> in <em>Phytophthora capsici</em> was investigated for its contribution to growth, pathogenicity, and reproduction of <em>P</em>. <em>capsici.</em> Corresponding results obtained showed that <em>PcLRR-RK2</em> is necessary for optimal vegetative growth of <em>P. capsici</em>, and silencing of <em>PcLRR-RK2</em> resulted in reduced vegetative growth. This study also demonstrated that silencing <em>PcLRR-RK2</em> causes defective zoosporogenesis, resulting in impaired sporangia production. Investigation further revealed that silencing <em>PcLRR-RK</em>2, which reduces the ability of zoospores to germinate and penetrate plant tissues, prevents the production of necrotic lesions indicative of disease establishment. Similarly, the mycelium of silenced <em>P. capsici</em> exhibited a deteriorated ability to cause infection and was unable to invade plant tissues extensively. In summary, our study suggested that <em>PcLRR-RK2</em> is necessary for regulating vegetative growth, thereby facilitating the penetration and infection of host leaf tissues by zoospores.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102884"},"PeriodicalIF":3.3,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144867416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and comprehensive characterization of the TGA gene family in pear fruit and their potential roles in response to Alternaria alternata infection","authors":"Zhaoyuan Wang ,&nbsp;Canying Li ,&nbsp;Wendan Qu ,&nbsp;Xin Fang ,&nbsp;Jiahui Cai ,&nbsp;Huixin Fang ,&nbsp;Yonghong Ge","doi":"10.1016/j.pmpp.2025.102882","DOIUrl":"10.1016/j.pmpp.2025.102882","url":null,"abstract":"<div><div>The black spot, caused by <em>Alternaria alternata</em>, represents a globally significant disease affecting pear fruit production. TGACG motif-binding factor (TGA) transcription factors, which are members of the basic region leucine zipper (bZIP) family, play a vital role in the defense mechanisms of plants against pathogens. This study systematically screened and characterized TGA genes in pears, and further investigated their potential roles in response to <em>A. alternata</em> infection. The results demonstrated that pears possess 10 TGA genes, which are distributed across 9 chromosomes and located in the nucleus. Phylogenetic analysis revealed that the 10 TGA genes can be classified into five distinct groups, each characterized by consistent structural features and motif compositions. The prediction of <em>cis</em>-regulatory elements indicates that <em>PbTGAs</em> are involved in responses to various hormones and environmental stimuli, as well as in growth and developmental processes. In addition, RT-qPCR analysis demonstrated that <em>PbTGA2.2</em>, <em>PbTGA2.3</em>, <em>PbTGA4</em>, <em>PbTGA7</em>, <em>PbTGA9</em>, and <em>PbTGA10</em> showed significant changes in expression levels following <em>A. alternata</em> infection. Furthermore, <em>A. alternata</em> infection was found to up-regulate the expression levels of <em>PbWRKY11/12/31/33</em>, <em>PbICS2</em>, <em>PbEPS1</em>, <em>PbEDS5</em>, <em>PbPBS3, PbNPR1</em>, and <em>PbPR-1</em> in Snow pear fruit. These results indicate that PbTGA genes play a role in the salicylic acid signaling pathway, contributing to resistance against <em>A. alternata</em> in pears.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102882"},"PeriodicalIF":3.3,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative defense profiling in wheat near-isogenic lines reveals mechanistic diversity among stripe rust resistance (Yr) genes conferring uniform infection type against Puccinia striiformis f. sp. tritici","authors":"K.K. Chetan ,&nbsp;Vaibhav Kumar Singh ,&nbsp;O.P. Gangwar ,&nbsp;Jayanth Kallugudi ,&nbsp;Sanjeev Sharma ,&nbsp;Bishnu Maya Bashyal ,&nbsp;M.S. Saharan ,&nbsp;M. Nishanth ,&nbsp;M. Bharani","doi":"10.1016/j.pmpp.2025.102881","DOIUrl":"10.1016/j.pmpp.2025.102881","url":null,"abstract":"<div><div>Wheat (<em>Triticum aestivum</em> L.), a chief staple food crop, faces significant production challenges due to <em>Puccinia striiformis</em> f. sp. <em>tritici</em> (<em>Pst</em>), responsible for stripe rust. Host plant resistance, being an effective disease management strategy, necessitates a deeper understanding of resistance mechanisms conferred by stripe rust-resistance (<em>Yr</em>) genes. In this study, near-isogenic lines (NILs) carrying different <em>Yr</em> genes (<em>Yr</em>5, <em>Yr</em>10, <em>Yr</em>15, <em>Yr</em>24, and <em>Yr</em>SP) displayed a uniform infection type (IT‘0’) upon inoculation with the emerging virulent <em>Pst</em> pathotype 238S119, yet exhibited divergent defense mechanisms. While spore germination and appressorium formation showed no differences, resistance divergence emerged during haustorial development (72–120 hpi). Early lignification and hypersensitive response (HR) were more pronounced in Avocet + <em>Yr</em>5, Avocet + <em>Yr</em>10, and Avocet + <em>Yr</em>24, while delayed callose deposition was gene-specific, with stronger accumulation in Avocet + <em>Yr</em>5 and Avocet + <em>Yr</em>15, highlighting variation in structural reinforcement against pathogen ingress. Biochemical profiling revealed <em>Yr</em> gene-specific contributions. Phenylalanine ammonia lyase (PAL) activity was highly induced in Avocet + <em>Yr</em>10, tyrosine ammonia lyase (TAL) in Avocet + <em>Yr</em>5, polyphenol oxidase (PPO) in Avocet + <em>Yr</em>15, and catalase (CAT) in Avocet + <em>Yr</em>24. Further, multivariate analysis showed that higher PAL, TAL, PPO, CAT, and phenolics were associated with resistance, while higher hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) levels correlated with susceptibility. These findings suggest the crucial role of phenylpropanoid metabolism in linking phenol to lignin biosynthesis and effective oxidative stress management in disease resistance. Despite similar external resistance, these <em>Yr</em> genes activate different structural and biochemical responses to block <em>Pst</em> infection. Understanding such diversity is critical for designing effective, durable resistance strategies in wheat breeding.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102881"},"PeriodicalIF":3.3,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144867415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential and mechanism of Bacillus velezensis YMG-03 for sesame diseases","authors":"Huaituo Yang ,&nbsp;Bipo He ,&nbsp;Xinbei Zhao ,&nbsp;Wenqing Yan ,&nbsp;Jing Wang ,&nbsp;Hui Zhao ,&nbsp;Yunxia Ni ,&nbsp;Hongyan Liu ,&nbsp;Chao Ma","doi":"10.1016/j.pmpp.2025.102880","DOIUrl":"10.1016/j.pmpp.2025.102880","url":null,"abstract":"<div><div>Sesame, a globally important oilseed crop, faces significant threats from fungal diseases, leading to substantial yield and quality losses. Utilizing antagonistic bacteria for biological control offers a sustainable and environmentally friendly alternative to chemical fungicides in sustainable agriculture. This study investigated the control effects and mechanisms of the <em>Bacillus velezensis</em> strain YMG-03 against sesame diseases. In dual-culture comparison assays, YMG-03 broadly inhibited eight major sesame pathogens, achieving 83.73% suppression against <em>Macrophomina phaseolina,</em> causing sesame stem blight. Scanning electron microscopy revealed severe morphological disruption of <em>M. phaseolina</em> hyphae following a dual-culture comparison assay with strain YMG-03. Pot experiments demonstrated that YMG-03 significantly suppressed stem blight disease and enhanced sesame growth. Genome analysis of strain YMG-03 revealed a 3.93 Mb circular chromosome containing 12 secondary metabolite biosynthetic gene clusters (<em>e.g.,</em> surfactin, fengycin, bacillibactin) and genes encoding hydrolytic enzymes (<em>e.g.,</em> chitinase, cellulase), indicating its genetic potential for antagonistic metabolite and hydrolases production. Untargeted metabolomics identified 1413 up-regulated metabolites, among which lipids (<em>e.g.,</em> liquiritigenin, jasmonic acid, capsaicin), organic acids (<em>e.g.,</em> 6-aminopenicillanic acid, piperic acid, coumaric acid), and key precursors for biocontrol metabolites (3-hydroxy fatty acids, N-acetylglucosamine, L-leucine) significantly accumulated. KEGG enrichment analysis revealed their association with fatty acid metabolism, polyketide/terpenoid biosynthesis, and amino sugar metabolism, consistent with the predicted antagonistic functions from genomic data. These findings systematically elucidate the dual role of <em>B. velezensis</em> YMG-03 in synergistically promoting sesame growth and controlling stem blight, and provide both strain resources and theoretical foundations for applying <em>B. velezensis</em> in biological control.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102880"},"PeriodicalIF":3.3,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144858103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics in plant-fungi interactions: Fundamental concepts to emerging innovations","authors":"Dhanabalan Shanmuga Priya ,&nbsp;Karuppiah Manikandan ,&nbsp;Iruthayasamy Johnson ,&nbsp;Chelladhurai Jeyalakshmi ,&nbsp;Rajamuthu Renuka ,&nbsp;Muthusamy Karthikeyan","doi":"10.1016/j.pmpp.2025.102878","DOIUrl":"10.1016/j.pmpp.2025.102878","url":null,"abstract":"<div><div>Plant-fungi interactions play a critical role in shaping agricultural productivity, with plant disease alone responsible for nearly 40 % of global crop losses annually. These interactions involve complex molecular dialogues between plants and pathogens, influencing disease progression and plant defense mechanisms. While genomics and transcriptomics have provided significant insights into these interactions, proteomics offers a dynamic perspective by capturing protein-level changes, post-translational modifications, and protein-protein interactions that directly mediate plant immunity and pathogen virulence. Recent breakthroughs in mass spectrometry, bioinformatics, and high-throughput proteomic techniques have significantly enhanced the resolution and accuracy of protein identification and functional characterization. The integration of proteomics with other omics approaches, such as genomics and metabolomics, has further enriched our understanding of plant defense responses, aiding in the development of disease-resistant crops. However, challenges such as proteome complexity, low-abundance protein detection, and data reproducibility remain obstacles in proteomic research. This review highlights the latest advancements in proteomic technologies, their application in deciphering host-pathogen interactions, and the potential future directions for integrating proteomics into sustainable disease management strategies. A deeper understanding of these mechanisms will facilitate the development of innovative crop protection strategies, ultimately contributing to global food security.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102878"},"PeriodicalIF":3.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144867419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and multilocus phylogenetic evidence confirms Calonectria pseudoreteaudii as the causal agent of leaf spot and blight on Manilkara hexandra in India","authors":"Sabyasachi Banerjee,&nbsp;Shweta Bhimta,&nbsp;Shailesh Pandey","doi":"10.1016/j.pmpp.2025.102877","DOIUrl":"10.1016/j.pmpp.2025.102877","url":null,"abstract":"<div><div><em>Manilkara hexandra</em> (Roxb.) Dubard (<em>Sapotaceae</em>), commonly known as Khirni or Rayan, is a tropical tree of significant medicinal and economic value in India. Since 2021, leaf spot and blight disease symptoms have been observed on <em>M. hexandra</em> trees, underscoring the need for accurate pathogen detection to enable timely and effective disease management. Symptomatic leaves consistently yielded fungal isolates identified as belonging to the genus <em>Calonectria</em>. Morphological characterization, combined with multilocus phylogenetic analyses of five partial gene regions—actin (<em>act</em>), calmodulin (<em>cmdA</em>), histone H3 (<em>his3</em>), translation elongation factor-1α (<em>tef1</em>), and β-tubulin (<em>tub2</em>)—identified the species as <em>Calonectria pseudoreteaudii</em>. Pathogenicity was validated through leaf inoculation assays, and Koch's postulates were fulfilled. This study represents the first report of <em>C. pseudoreteaudii</em> infecting <em>M. hexandra</em> in India and worldwide and highlights the need for further research on the diversity and cross-infectivity of <em>Calonectria</em> species to inform effective management strategies.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102877"},"PeriodicalIF":3.3,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and molecular characterization of Podosphaera xanthii associated with powdery mildew of betel leaves (Piper betle L.) – a new record from India","authors":"Shivannegowda Mahadevakumar ,&nbsp;Helan Baby Thomas ,&nbsp;Kadaiah Ajithkumar ,&nbsp;Attihalli Shivalingegowda Savitha ,&nbsp;Mulpuri Sujatha ,&nbsp;Madappa Mahesh ,&nbsp;Sajeewa S.N. Maharachchikumbura ,&nbsp;Marikunte Yenjarappa Sreenivasa","doi":"10.1016/j.pmpp.2025.102875","DOIUrl":"10.1016/j.pmpp.2025.102875","url":null,"abstract":"<div><div>Betel leaf (<em>Piper betle</em>), a medicinally and economically valuable climber, is widely cultivated in Karnataka, India. During field investigations in 2023–24, the distinctive powdery mildew signs were noted on both young and adult betel vine leaves. Estimates of the disease severity ranged from 5 to 34 %. Representative plant samples affected with powdery mildew were gathered and were identified using microscopic observations. The powdery mildew fungus was identified as a species of <em>Podosphaera</em> after morphological analysis. Microscopic and morphological examinations corroborated the identification. Six representative samples were chosen, and their ITS-rDNA regions were amplified and sequenced to confirm the identity. The ITS-rDNA sequence alignment was used for the phylogenetic analysis. The ITS region sequence data shared the highest degree of homology with reference sequences of <em>Podosphaera xanthii</em>, according to the results of the nBLAST sequence analysis. Additionally, phylogenetic study was carried out to establish the identity. Based on ITS-rDNA sequence information, phylogeny, and micro-morphological attributes, the associated pathogen was confirmed as <em>Podosphaera xanthii</em>. Severe infections resulted in reduced leaf yield, posing risks to betel farmers. To the best of our knowledge, this is the first time that <em>P. xanthii</em> has been associated with <em>P. betle</em> powdery mildew disease in India.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102875"},"PeriodicalIF":3.3,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial dysbiosis and functional decline drive root-knot nematode outbreaks in continuous cropping systems","authors":"Xinyan Wang ,&nbsp;Lihui Tan ,&nbsp;Yanzhuo Liu ,&nbsp;Yongzhong Wang ,&nbsp;Hengqian Lu","doi":"10.1016/j.pmpp.2025.102876","DOIUrl":"10.1016/j.pmpp.2025.102876","url":null,"abstract":"<div><div>The long-term practice of continuous cropping has been demonstrated to have a deleterious effect on the incidence of root-knot nematode disease. However, the extent to which the relationship among continuous cropping, root-knot nematode disease, and soil microbial community are understood remains limited. The present study systematically comparted physicochemical properties and microbial communities of disease-conducive soil from long-term continuous cropping systems and healthy soil. The results revealed that continuous cropping led to significant decreases in soil organic carbon, available nitrogen, and available potassium content, while simultaneously inducing changes in microbial α- and β-diversity. The analysis of microbial community composition revealed that disease-conducive soil exhibited a significant decrease in the abundance of potentially beneficial <em>Actinobacteriota</em>, <em>Streptomyces</em> and <em>Bacillus</em>, along with an abnormal enrichment of <em>Acidobacteriota</em>. Functional prediction based on FAPROTAX, Tax4Fun2 and BugBase analysis revealed impaired nitrogen fixation, nitrate denitrification, and nitrite denitrification and compromised methanol oxidation and methylotrophy in disease-conducive soil, disease-conducive soil also exhibits weaker capabilities in lipid metabolism, amino acid metabolism and secondary metabolite production compared to healthy soil, disease-conducive soil was enriched with aerobic Gram-negative bacteria, contrasting with healthy soil dominated by facultative anaerobic Gram-positive bacteria with higher contains mobile elements. The correlation analysis confirmed significant interactions among key microbial taxa, root-knot nematode biocontrol efficacy, and soil physicochemical properties. These findings demonstrate that long-term continuous cropping promotes root-knot nematode disease by disrupting soil nutrients, suppressing beneficial microbes, and impairing key metabolic functions, providing crucial insights for soil health management and biological disease control strategies.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102876"},"PeriodicalIF":3.3,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}