Morphological and molecular characterization of Podosphaera xanthii associated with powdery mildew of betel leaves (Piper betle L.) – a new record from India

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-08-11 DOI:10.1016/j.pmpp.2025.102875

Shivannegowda Mahadevakumar , Helan Baby Thomas , Kadaiah Ajithkumar , Attihalli Shivalingegowda Savitha , Mulpuri Sujatha , Madappa Mahesh , Sajeewa S.N. Maharachchikumbura , Marikunte Yenjarappa Sreenivasa

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引用次数: 0

Abstract

Betel leaf (Piper betle), a medicinally and economically valuable climber, is widely cultivated in Karnataka, India. During field investigations in 2023–24, the distinctive powdery mildew signs were noted on both young and adult betel vine leaves. Estimates of the disease severity ranged from 5 to 34 %. Representative plant samples affected with powdery mildew were gathered and were identified using microscopic observations. The powdery mildew fungus was identified as a species of Podosphaera after morphological analysis. Microscopic and morphological examinations corroborated the identification. Six representative samples were chosen, and their ITS-rDNA regions were amplified and sequenced to confirm the identity. The ITS-rDNA sequence alignment was used for the phylogenetic analysis. The ITS region sequence data shared the highest degree of homology with reference sequences of Podosphaera xanthii, according to the results of the nBLAST sequence analysis. Additionally, phylogenetic study was carried out to establish the identity. Based on ITS-rDNA sequence information, phylogeny, and micro-morphological attributes, the associated pathogen was confirmed as Podosphaera xanthii. Severe infections resulted in reduced leaf yield, posing risks to betel farmers. To the best of our knowledge, this is the first time that P. xanthii has been associated with P. betle powdery mildew disease in India.

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印度槟榔叶白粉病新记录——黄氏Podosphaera xanthii的形态和分子特征

槟榔叶（Piper betle）是一种具有药用和经济价值的攀援植物，广泛种植于印度卡纳塔克邦。在2023 - 2024年的实地调查中，在幼嫩和成年槟榔藤叶子上都发现了独特的白粉病迹象。对疾病严重程度的估计从5%到34%不等。收集了受白粉病影响的代表性植物样品，并利用显微镜观察进行了鉴定。经形态学分析，确定该白粉菌属Podosphaera。显微镜和形态学检查证实了这一鉴定。选取6个有代表性的样本，对其ITS-rDNA区域进行扩增和测序以确认其身份。采用ITS-rDNA序列比对进行系统发育分析。根据nBLAST序列分析结果，ITS区域序列数据与黄氏Podosphaera xanthii参考序列具有最高的同源性。此外，还进行了系统发育研究以确定其身份。根据ITS-rDNA序列信息、系统发育和微形态特征，确定相关病原菌为黄氏Podosphaera xanthii。严重的感染导致叶片产量下降，给槟榔种植者带来风险。据我们所知，这是第一次在印度发现黄氏假单胞菌与白粉病有关。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.