Physiological and Molecular Plant Pathology最新文献

{"title":"Mechanism of action of isobavachalcone in inducing resistance to tobacco mosaic virus disease in tobacco","authors":"","doi":"10.1016/j.pmpp.2024.102450","DOIUrl":"10.1016/j.pmpp.2024.102450","url":null,"abstract":"<div><div>Isobavachalcone (IBC), a natural active compound derived from the seeds of <em>Psoralea corylifolia</em> L., is a botanical fungicide that has been shown to effectively manage various plant diseases caused by pathogenic fungi, bacteria, and viruses in agricultural settings. However, the mechanism by which IBC induces tobacco resistance to tobacco mosaic virus (TMV) infection remains unclear. In this study, tobacco plants at the 6～8 leaf stage were treated with 40 mg/L IBC three times before inoculation with TMV. At 24 h post-inoculation, differentially expressed genes between the IBC-treated group and the inoculated control group were mainly enriched in plant hormone signal transduction and MAPK(mitogen-activated protein kinase)signaling pathways. The expression levels of positive regulatory factors <em>JAR1</em>, <em>SnRK2</em>, <em>ABF</em>, <em>FLS2</em>, and <em>PR</em> in the above signaling pathways were significantly up-regulated, while the expression level of the negative regulatory factor <em>JAZ</em> was significantly down-regulated. From 1 to 9 days post-inoculation, the activities of four defense enzymes, SOD, PPO, PAL, and POD, in the IBC-treated tobacco leaves were significantly higher than those in the inoculated control. These results suggest that IBC activates the MAPK signaling pathway in plants, regulates the coordination of SA, JA, and ABA plant hormone signaling networks in immune responses, and increases the activities of pathogenesis-related proteins and defense enzymes in tobacco to induce resistance against TMV. This study reveals the mechanism of IBC-induced resistance against TMV in tobacco at the transcriptomic and physiological-biochemical levels, providing a theoretical basis for the widespread application of IBC in agriculture.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring nonanol from Bacillus velezensis (YEBBR6) for root-knot nematode management in bananas: Integrating experimental and computational approaches","authors":"","doi":"10.1016/j.pmpp.2024.102452","DOIUrl":"10.1016/j.pmpp.2024.102452","url":null,"abstract":"<div><div>Nematode infestations pose a severe threat to global banana production, leading to substantial yield losses and economic damage. Among these, the root-knot nematode <em>Meloidogyne incognita</em> is particularly detrimental, causing root galls that severely impair water and nutrient uptake, leading to stunted growth, reduced fruit quality, and significant economic losses for farmers. In the face of increasing agricultural challenges, bacterial endophytes have emerged as essential tools for sustainable agriculture, offering ecofriendly solutions to enhance plant health and manage pests. The present study aims to identify and characterise biomolecules from bacterial endophyte <em>Bacillus velezensis</em> (YEBBR6) with potential nematicidal properties against root-knot nematode <em>Meloidogyne incognita</em> infecting banana. The analysis of volatile and non-volatile organic compounds (VOCs/NVOCs) from the inhibition zone during the ditrophic interaction between <em>B. velezensis</em> (YEBBR6) and <em>Fusarium oxysporum</em> f.sp. <em>cubense</em> (Foc) revealed the presence of several distinctive biomolecules with antifungal and antimicrobial properties. These compounds included linoelaedic acid, nonanol, acetylvaleryl, 5–hydroxyl methyl furfural, clindamycin, allobarbital, 3-thiazolidine carboxamide, azulene, aminomorpholine, procyclidine, campholic acid, 3 amino-4 hydroxy phenyl sulfone, 3–deoxy mannoic lactone, hexadecanoic acid, oleic acid, and dihydroacridine. The <em>in-silico</em> analysis revealed that nonanol, exhibited strong binding efficacy against <em>M. incognita</em> protein targets, including cytochrome C oxidase subunit 1, calreticulin, neuropeptide G-protein coupled receptor, chorismate mutase 1, venom allergen-like proteins, and β-1,4-endoglucanase. Notably, nonanol proved more effective than the commercially used nematicide carbofuran 3G. Molecular dynamics studies further supported the potential of nonanol, and its nematicidal properties were validated through in vitro and pot culture studies. Moreover, nonanol was found to induce the expression of defense-related genes, such as MAPK10, WRKY22, ascorbate peroxidase, catalase, and NADPH oxidase, in banana seedlings, thus enhancing the plant's immune response. These findings highlight the potential of nonanol, derived from <em>B. velezensis</em> (YEBBR6), as a potent and environmentally friendly alternative for controlling nematode infestations in banana cultivation.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-biochemical and molecular characterization of new mung bean [Vigna radiata (L.) Wilczek] landraces for Cercospora leaf spot (CLS) disease resistance","authors":"","doi":"10.1016/j.pmpp.2024.102448","DOIUrl":"10.1016/j.pmpp.2024.102448","url":null,"abstract":"<div><div>Mung bean production is significantly lowered by <em>Cercospora</em> leaf spot (CLS) in various parts of the globe. The most effective way to increase mung bean resilience to this stress is to identify new CLS resistance sources. Therefore, a panel population of 13 landraces along with 2 susceptible check cultivar, and 11 SSR markers were taken for the present experiment. Correlation analysis suggested significant relationships between the quantitative traits, retained resistance (Banapur local, and Kalampur local), and moderately resistance (Dikhitapada local, and Fakirpur local 1) to CLS. However, total chlorophyll content (TCL) showed strong positive correlations with phenolic content (PHE) (0.984), peroxidase activity (POX) (0.931), and polyphenol oxidase activity (PPO) (0.969). Moreover, PHE (−0.769) and POX (−0.867) were also found negatively correlated with CLS disease reaction. The eigenvalues from the principal component analysis (PCA) indicated the first three principal components collectively explained 84.88 % of the total variance. Molecular analysis of the panel population suggested a PIC value range from 0.30 to 0.88, with an average 0.696, and markers such as cp01713 (0.84), cp02181 (0.87), and CEDG147 (0.88) had higher PIC values. Principal coordinate analysis (PCoA) suggested capturing of 69.06 % of the total variance by the first three axes, and the landraces were categorizing into three clusters (cluster A, B and C). The genetic distances among the landraces ranged from 0 to 0.608, and phylogenetic tree differentiated them into three major clusters at respective Jaccard's similarity coefficient <em>i.e.,</em> Cluster I (0.68), II (0.75) and III (0.83). Meanwhile, the resistance and moderately resistance landraces were observed in cluster A and cluster B, and in cluster II and cluster III respectively. The resistance landraces obtained from the present study could be used as potential donor for breeding process towards CLS resistance in mung bean.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of nicotinamide from Arachis hypogaea roots against Meloidogyne incognita","authors":"","doi":"10.1016/j.pmpp.2024.102455","DOIUrl":"10.1016/j.pmpp.2024.102455","url":null,"abstract":"<div><div>Root knot nematode disease is a global problem that causes significant economic losses to cash crops annually, and <em>Meloidogyne incognita</em> is the most destructive threat. The urgent need for reliable biocontrol agents is evident. This study evaluated the nematicidal effects of various tissue extracts from <em>Arachis hypogaea</em>, a non-host plant of <em>M</em>. <em>incognita</em>. Subsequently, the metabolic changes in peanut roots induced by <em>M</em>. <em>incognita</em> were analyzed, and the in vitro biological activity of differential metabolites were tested. The results indicated that peanut root extracts showed the highest inhibiting effect on <em>M</em>. <em>incognita</em>, the LC₅₀ values were 18.54 mg/mL, 11.58 mg/mL, and 8.05 mg/mL after treated 24, 36, and 48 h, respectively. Additionally, it was found that <em>M</em>. <em>incognita</em> infestation induced distinct metabolic changes in peanut roots. Among the differential metabolites, nicotinamide exhibited strong nematicidal effects on <em>M. incognita</em>, and the corrected mortality of second-stage juveniles and hatching inhibition of eggs could reach 99.65 % and 95.90 %, respectively at a concentration of 25 mg/mL. In conclusion, nicotinamide manifested distinctive advantages as a novel nematicide for the potential applications in sustainable agriculture.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of bacterial blotch disease caused by Burkholderia gladioli in Grifola frondosa from China","authors":"","doi":"10.1016/j.pmpp.2024.102458","DOIUrl":"10.1016/j.pmpp.2024.102458","url":null,"abstract":"<div><div><em>Grifola frondosa</em> has been regarded as healthy food resources for its potentially nutraceutical and pharmaceutical values for centuries. During 2023, a bacterial blotch disease with a 40 % incidence rate was observed in <em>G</em>. <em>frondosa</em> in Qingyuan County, Zhejiang Province, China. The strain isolated from symptomatic tissues was identified as <em>Burkholderia gladioli</em> through a combination of morphological characteristics, biochemical features, and phylogenetic analysis. This is the first documented case of <em>B</em>. <em>gladioli</em> causing bacterial blotch in <em>G</em>. <em>frondosa</em> in China. This research will deepen the understanding and provide a reference for available management of this disease in field.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New emerging Pseudomonads as causal agents of bean blight disease","authors":"","doi":"10.1016/j.pmpp.2024.102430","DOIUrl":"10.1016/j.pmpp.2024.102430","url":null,"abstract":"<div><div>Common bean (<em>Phaseolus vulgaris</em>) production is negatively affected by several bacterial plant diseases. A severe outbreak of bean blight was observed in the nine major bean-producing provinces of Iran during 2021–2022. Necrotic spots were observed on leaves which become necrotic with chlorosis beyond it. One hundred-sixty bacterial strains with a greyish-white colony appearance were consistently isolated from the one-hundred eighteen symptomatic bean leaf, pod, seed and root samples on nutrient agar. All strains were screened for their ability to cause disease bean leaves among which 134 strains induced chlorosis three days after inoculation on bean leaves of cv. Yaghoot followed by necrotic spots on the inoculated sites one week after inoculation. The pathogenic strains clustered in three groups based on phenotypic characters and multilocus sequencing analyses. Three housekeeping genes including <em>gyrB</em>, <em>rpoB</em>, and <em>rpoD</em> of representative strains were partially sequenced. In the phylogenetic tree based on concatenated sequences of these three genes or each gene individually, the strains were divided into three clusters each with high bootstrap support. The first group of the strains clustered with <em>Pseudomonas simiae</em> CCUG 50988^T. This is the first report of this species as causal agent of bean blight. The second group clustered close to <em>Pseudomonas alloputida</em> NMI5768^T which apparently belong to a new species. The third group clustered in a separate clade in <em>Pseudomonas chlororaphis</em> species which belongs to a new subspecies based on pathogenicity on bean, phenotypic characters, sequences of five housekeeping genes which named as subsp. <em>phaseoli</em> subsp. nov. with the type strain UT-M315.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly of phyllosphere bacterial community with PsnWRKY70 in poplar","authors":"","doi":"10.1016/j.pmpp.2024.102461","DOIUrl":"10.1016/j.pmpp.2024.102461","url":null,"abstract":"<div><div>Plant microbiome plays an important role in growth and development, biotic and abiotic stresses, and genotype is one of the key factors in shaping the microbiome. In this study, the effect of <em>PsnWRKY70</em> on the synthetic bacterial community of poplar was investigated. To explore the assembly mechanism of poplar phyllosphere bacteria. we used 39 strains of bacteria isolated from the phyllosphere of poplar to construct a synthetic bacterial community. The leaves of aseptic poplar tissue culture seedlings were inoculated with synthetic bacterial community, and the bacterial communities of poplar leaves inoculated with synthetic bacterial community for 0 d, 5 d, 10 d and 15d were sequenced for 16 s. Bacterial community composition, PCoA, and Lefse analyses were performed. The results showed that the bacterial community in the phyllosphere of poplar tended to be in a stable equilibrium state after inoculation with the synthetic bacterial community. The results of β diversity analysis showed that there were significant differences in the bacterial community structure of OE, RE and WT leaves at 5d and 10 d after inoculation with Syncom. It is mainly composed of <em>Methylobacteriaceae</em>, <em>Enterbacteriaceae</em>, <em>Agrobacterium</em>, <em>Rhizobium</em>, <em>Pseudoxanthomonas</em> and <em>Stenotrophomonas</em>. The results of differential bacterial analysis showed that <em>Paracoccus</em>, <em>Arthrobacter</em> and <em>Rhodococcus</em> had higher relative abundance and stability in OE line. <em>PsnWRKY70</em> gene plays an important role in recruiting <em>Paracoccus</em>, <em>Arthrobacter</em> and <em>Rhodococcus</em> strains in poplar leaves.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the non-chemical alternatives for the management of post-harvest fungal diseases of major tropical fruits- mango, banana and papaya","authors":"","doi":"10.1016/j.pmpp.2024.102460","DOIUrl":"10.1016/j.pmpp.2024.102460","url":null,"abstract":"<div><div>Fruits plays an important role in the balanced diet of mankind. The greatest challenge faced by the fruit sector is the post-harvest losses, which questions the availability of the fruits in contrast to the volume of production. Post-harvest losses was estimated as 5–20 % in developed countries while 20–50 % in developing countries. Among the numerous aliments affecting the post-harvest industry, the role played by the fungal pathogens are highly significant. Anthracnose, stem end rot, <em>Rhizopus</em> soft rot, gray mold, <em>etc</em> are important diseases contributing to huge losses in tropical fruits. The chemical method of disease control is not recommended as it is evoking severe health concerns among the consumers. Thus, non-chemical alternatives have to gain momentum and should replace the synthetic fungicides. Some of the emerging alternatives for the effective management of post-harvest fungal diseases include the use of edible coatings, essential oils, GRAS salts, botanicals, heat treatment, irradiation, ROS and biological control. Many of these methods exhibit effectiveness comparable with the chemical control.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of biochemical compounds, chemical elements and metabolites modifications in maize plants infected with maize rayado fino virus (MRFV)","authors":"","doi":"10.1016/j.pmpp.2024.102457","DOIUrl":"10.1016/j.pmpp.2024.102457","url":null,"abstract":"<div><div>Maize cultivation is crucial worldwide, especially in Latin America. However, diseases related to corn stunt complex have significantly impacted crops, reducing grain productivity and quality. Maize rayado fino virus (MRFV) is part of this complex, and its symptom is characterized by chlorotic dots along veins and, depending on maize genotype, there will be stunting and an impact on the corn cob development, which can lead to productivity losses. However, this intricate plant-pathogen relationship is yet not well understood. Here, we investigated the effects of MRFV transmitted by the corn leafhopper <em>Dalbulus maidis</em> in infected plants in their early stage of development. To show the impact of the viral infection in the maize plant, we performed biochemical and chemical analysis and nuclear magnetic resonance (NMR). The total set of analyses showed that changes in chemical and biochemical compounds, as well as in metabolites composition and activities, can be perceived in MRFV-infected maize plants when compared to healthy plants. An increase in the activity of superoxide dismutase, catalase, peroxidase, and β-glucanase was detected, whereas small changes have been identified in antioxidant activity and phenolic compounds. For chemical components, unique changes were observed, mainly the increase in the presence of some elements such as calcium (Ca), phosphorus (P), sodium (Na), and iron (Fe). We identified 20 metabolites of the amino acids, organic compounds, and carbohydrates classes in maize plants which are regulated during MRFV infection. Maize plants reacted to <em>D. maidis</em> herbivory by modulating both enzymatic and non-enzymatic activities, as well as chemical compounds. This research offers insights into the responses of maize against MRFV and sheds light on parameters to be used on the search of rayado fino viral disease resistance for a sustainable production.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Colletotrichum perseae Causing Anthracnose Disease of Avocado (Persea americana) in Türkiye","authors":"","doi":"10.1016/j.pmpp.2024.102454","DOIUrl":"10.1016/j.pmpp.2024.102454","url":null,"abstract":"<div><div><em>Colletotrichum</em>-induced anthracnose is a major disease of avocado (<em>Persea americana</em>), causing significant losses in production worldwide. In 2023, a survey of avocado fruits in markets within the Kızıltepe district of Mardin, Türkiye, revealed symptoms of anthracnose, characterized by brown-black lesions and internal necrosis. To identify the causal agent, 20 symptomatic avocado fruits were collected and fungal isolates resembling <em>Colletotrichum</em> species were obtained. Morphological characterization, multilocus sequence analysis of the internal transcribed spacer (ITS), actin (<em>act</em>), the <em>apn2-Mat1-2</em> intergenic spacer (<em>ApMat</em>), and calmodulin (<em>cal</em>) loci, and pathogenicity testing were performed for only two representative isolates (Pa01 and Pa02). These analyses identified the pathogen as <em>Colletotrichum perseae</em>. The isolates readily formed ascomata on all culture media tested, suggesting a high capacity for sexual reproduction. Pathogenicity tests confirmed that <em>C. perseae</em> caused characteristic dark, sunken anthracnose lesions on avocado fruits. This is the first report of <em>C. perseae</em> causing anthracnose in avocados in Türkiye, expanding the known geographical distribution of this pathogen. The findings highlight the need for effective disease management strategies and further research to understand the pathogen's lifecycle, environmental conditions favoring infection, and potential for seedborne transmission.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}