Transcriptome analysis of Nicotiana tabacum in response to Pseudomonas syringae pv. tabaci infection

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-09-26 DOI:10.1016/j.pmpp.2025.102965

Xiaojie Ding , Ao Jing , Dongyang Liu , Chuantao Xu , Fei Wang , Chong Zhang , Rujun Zhou , Yuanhua Wu

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Abstract

Tobacco wildfire disease, caused by Pseudomonas syringae pv. tabaci, severely reduces tobacco yield and quality, yet the host response remains poorly understood. Here, a comprehensive transcriptomic analysis of Nicotiana tabacum infected with P. syringae pv. tabaci YH3 was performed at four developmental stages. Among the top 12 KEGG pathway enrichment analysis, the differentially expressed genes (DEGs) involved in the MAPK signaling pathway-plant, carbon fixation in photosynthetic organisms, plant hormone signal transduction, ribosome, plant-pathogen interaction, and RNA degradation were significantly enriched at three time points. Notably, among the identified DEGs, leucine-rich repeat receptor-like protein kinase (LRR-RLK), indole-3-acetic acid (IAA), and cysteine synthase (CS) emerged as potential key regulatory genes involved in the tobacco response to P. syringae pv. tabaci YH3 infection. These findings provide a molecular framework for understanding the transcriptomic response of N. tabacum to P. syringae pv. tabaci infection and offer promising candidate genes for investigations into the interaction mechanisms between this pathogen and its host plants.

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烟草对丁香假单胞菌应答的转录组分析。烟感染

由丁香假单胞菌引起的烟草野火病。烟粉虱严重降低烟草产量和质量，但对寄主的反应仍知之甚少。本文对烟草感染丁香假单胞菌的转录组学进行了全面分析。烟草YH3在四个发育阶段进行。在前12个KEGG通路富集分析中，参与MAPK信号通路-植物、光合生物固碳、植物激素信号转导、核糖体、植物-病原体相互作用和RNA降解的差异表达基因（DEGs）在3个时间点显著富集。值得注意的是，在鉴定的DEGs中，富含亮氨酸的重复受体样蛋白激酶（LRR-RLK）、吲哚-3-乙酸（IAA）和半胱氨酸合成酶（CS）被认为是参与烟草对紫丁香疫病反应的潜在关键调控基因。YH3型烟粉虱感染。这些发现为了解烟草对丁香疫病毒的转录组反应提供了分子框架。为研究烟粉虱与寄主植物的相互作用机制提供了有希望的候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.