Physiological and Molecular Plant Pathology最新文献

{"title":"Identifying fungi responsible for trunk and scaffold diseases in almonds in Türkiye","authors":"Emel Ören ,&nbsp;Harun Bayraktar","doi":"10.1016/j.pmpp.2025.102729","DOIUrl":"10.1016/j.pmpp.2025.102729","url":null,"abstract":"<div><div>A comprehensive survey was conducted between 2020 and 2021 to identify fungal pathogens contributing to almond decline in the Southeastern Anatolia Region of Türkiye. Symptomatic samples were collected from 161 orchards across three provinces, targeting trunks and branches displaying gummosis, stem cankers, branch dieback, wilting, vascular discoloration, and necrosis. In total, 324 samples exhibiting canker symptoms were processed, yielding 196 fungal isolates belonging to 14 species. Morphological characterization and DNA sequence analyses identified <em>Botryosphaeria dothidea, Neoscytalidium dimidiatum, Macrophomina phaseolina, Clonostachys rosea, Fusarium brachygibbosum, F. oxysporum, F. proliferatum, F. redolens, F. solani, F. acuminatum, F. verticillioides, Aspergillus flavus, Paecilomyces maximus,</em> and <em>Verticillium dahliae. F. proliferatum</em> was the dominant species (33.2 % prevalence), followed by <em>V. dahliae, N. dimidiatum,</em> and <em>F. verticillioides.</em> Pathogenicity assays revealed that all fungal species were capable of inducing necrotic lesions at inoculation points on branch segments and seedlings. This study documented the first occurrence of <em>F. verticillioides, P. maximus, C. rosea,</em> and <em>A. flavus</em> on almond, as well as the first reports of <em>F. oxysporum, F. proliferatum, F. redolens, F. acuminatum, F. brachygibbosum,</em> and <em>V. dahliae</em> in association with almond trees in Türkiye.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102729"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environment disturbances influence bacterial diversity, structure and co-occurrence network in the Digitaria sanguinalis rhizosphere","authors":"Yiming Dai ,&nbsp;Ye Cui ,&nbsp;Qingchen Xiao ,&nbsp;Meiling Ping ,&nbsp;Yiming Zhang ,&nbsp;Shiguang Hu ,&nbsp;Hao Chen ,&nbsp;Xiaoyu Li ,&nbsp;Jin Chen","doi":"10.1016/j.pmpp.2025.102716","DOIUrl":"10.1016/j.pmpp.2025.102716","url":null,"abstract":"<div><div>Physicochemical properties of the soil and vegetation types exert primary influences on soil microorganisms, yet there is limited research on the impacts of two different levels of disturbance, especially in the rhizosphere of the <em>Digitaria sanguinalis</em>. To fill this knowledge gap, we employed the Illumina Miseq high-throughput sequencing technology to obtain 16S rRNA from the rhizosphere in D. sanguinalis soil near and away from the roadside. The rhizosphere soil microorganisms in the two locations experienced two different levels of disturbance and exhibited notable diversity. Meanwhile, there has been a marked rise in the abundance of the Acidobacteria genus <em>RB41</em> within this lightly disturbed region. The analysis of the co-occurrence network suggests that lightly disturbed areas exhibit the presence of two keystone taxa. In contrast to the heavily disturbed site (HD), the lightly disturbed site (LD) exhibits an increased level of the modular network (0.482 vs. 0.448) and stronger competition within the rhizosphere soil microbial community (positive/negative link ratio, P/N: 0.881 vs. 0.864). The findings from the PLS-SM analysis reveal that keystone taxa have the most total effects on aboveground biomass (0.957). These discoveries have important implications for understanding how different levels of disruption impact soil ecosystems.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102716"},"PeriodicalIF":2.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new host record of Phacidium calderae on Citrus limon leaves, causing necrotic spots and patches, in Algeria","authors":"Ali Kerroum ,&nbsp;Abdelmoumen Taoutaou ,&nbsp;Rossana Parlascino ,&nbsp;Mario Riolo ,&nbsp;Abdenour Zibani ,&nbsp;Hanane Djabali ,&nbsp;Zouaoui Bouznad ,&nbsp;Santa Olga Cacciola","doi":"10.1016/j.pmpp.2025.102728","DOIUrl":"10.1016/j.pmpp.2025.102728","url":null,"abstract":"<div><div>This study reports, for the first time, <em>Phacidium calderae</em> as the causal agent of necrotic lesions on <em>Citrus limon</em> leaves in Algeria. Symptomatic leaves showed white leathery patches with reddish margins and lenticular pustules. Fungal isolates obtained from lesions were morphologically and molecularly identified as <em>P. calderae</em>. Pathogenicity tests confirmed its ability to induce necrotic lesions on lemon leaves, although only in wounded tissues, suggesting low aggressiveness on citrus. However, <em>P. calderae</em> caused severe rot in artificially inoculated apple and pear fruit, revealing a broader host range. This finding is particularly relevant given that <em>Phacidium lacerum</em> was previously identified as a postharvest pathogen of apples and pears. The ability of <em>P. calderae</em> to infect multiple hosts raises concerns about its epidemiology and potential impact on fruit production. To our knowledge, this is the first report of <em>P. calderae</em> on citrus. Further research is needed to clarify its ecology, epidemiology, and interactions with other citrus pathogens.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102728"},"PeriodicalIF":2.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Yersiniaceae bacteria in plant disease suppression: Emerging biocontrol agents","authors":"Ma del Carmen Orozco-Mosqueda ,&nbsp;Luzmaria R. Morales-Cedeño ,&nbsp;Olubukola Oluranti Babalola ,&nbsp;Gerardo Puopolo ,&nbsp;Gustavo Santoyo","doi":"10.1016/j.pmpp.2025.102720","DOIUrl":"10.1016/j.pmpp.2025.102720","url":null,"abstract":"<div><div>The immoderate application of agrochemicals worldwide has led to the need to find safer disease control strategies for humans, animals, plants, and the environment. The use of microbial biocontrol agents addresses this need effectively, yet further efforts are required to expand the repertoire of microorganisms that can be developed as commercial biocontrol agents. Among these, a set of bacteria from the <em>Yersiniaceae</em> family has emerged as a promising option, offering both biocontrol potential and direct plant growth promotion. Genera like <em>Rahnella</em>, <em>Rouxiella</em>, and <em>Serratia</em> exhibit multiple mechanisms that contribute to plant health, but their role in biocontrol remains underexplored compared to other bacterial groups. This review aims to bridge this knowledge gap by examining the biocontrol capabilities, mechanisms, strengths, and areas of opportunity of <em>Yersiniaceae</em> bacteria, emphasizing their role in sustainable agriculture and their potential for future applications in this dynamic field.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102720"},"PeriodicalIF":2.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil-borne fungal pathogens of sesame: insights into pathogenesis and control","authors":"Abdelaaziz Farhaoui ,&nbsp;Mohamed Kouighat ,&nbsp;Mohammed Taoussi ,&nbsp;Rachid Lahlali","doi":"10.1016/j.pmpp.2025.102726","DOIUrl":"10.1016/j.pmpp.2025.102726","url":null,"abstract":"<div><div>Sesame (<em>Sesamum indicum</em>), nicknamed the queen of oilseeds, is a globally important oilseed crop, recognized for its culinary, industrial, and medicinal uses. However, its production is seriously threatened by several soil-borne fungal pathogens such as <em>Macrophomina phaseolina</em>, <em>Fusarium oxysporum</em> f.sp. <em>Sesami</em>, <em>Corynespora cassiicola</em>, and <em>Pythium</em> spp. These fungi pose serious threats to the quality of the harvest as well as the yield of this crop. This review provides an in-depth overview of the biology, genetic diversity, and growth requirements of sesame, before focusing on the major soil-borne fungal pathogens affecting this plant, their symptoms, and epidemiology. The main molecular tools allowing the characterization of these phytopathogens are also discussed. Management approaches including integrated disease management (IDM), biological control, cultural practices, and chemical controls are reviewed in detail. This review also examines the progress made in genetic resistance mechanisms and breeding programs for sesame varieties that are resistant to these fungi. Innovative approaches such as CRISPR-Cas9 and RNA interference (RNAi) are also discussed in the context of improving the management of soil-borne pathogens. This review highlights the importance of sustainable procedures to control soil-borne phytopathogens and optimize sesame health and productivity.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102726"},"PeriodicalIF":2.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biocontrol potential of Bacillus thuringiensis against Colletotrichum gloeosporioides in 'Hass' avocado fruits","authors":"Claudia Gabriela Magallón-Andalón ,&nbsp;Montserrat Calderón-Santoyo ,&nbsp;Rosendo Balois-Morales ,&nbsp;Verónica Alhelí Ochoa-Jiménez ,&nbsp;Paloma Patricia Casas-Junco ,&nbsp;Graciela Guadalupe López-Guzmán ,&nbsp;Iza Fernanda Pérez-Ramírez ,&nbsp;Carla Fabiola Arias-Navarro ,&nbsp;Pedro Ulises Bautista‐Rosales","doi":"10.1016/j.pmpp.2025.102724","DOIUrl":"10.1016/j.pmpp.2025.102724","url":null,"abstract":"<div><div>Bacteria of the <em>Bacillus</em> genus are considered ideal biocontrol agents because they have different ways of controlling certain pathogens and do not affect fruits such as avocados. This study aimed to determine biocontrol potential of <em>Bacillus thuringiensis</em> strains AB7, AB21 and AB30 against <em>Colletotrichum gloeosporioides</em> in 'Hass' avocado fruits. In the mechanism of action competition for nutrients, all strains exerted this mechanism for the glucose substrate. All strains tested showed competition for space against the pathogen <em>C. gloeosporioides</em>. Regarding the production of volatile organic compounds, the three strains managed to inhibit the growth of the pathogen due to the production of volatiles. Regarding the production of antibiotics, none of the three strains controlled the growth of the pathogen significantly, possibly due to a low production of antimicrobial compounds. Finally, for parasitism, the three strains produced the hydrolytic enzyme β-1,3-glucanase and adhered to the pathogen's mycelium. The three strains managed to cause deformations in the fungus hyphae, possibly due to the joint action of the adhesion of the biocontrol agents to the pathogen, the volatile organic compounds, the enzyme β-1,3-glucanase and some antibiotics that the strains produced in low concentrations. The evaluated strains inhibited <em>C. gloeosporioides</em> by using at least four action mechanisms.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102724"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143903826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylic acid-induced defense responses against a newly identified cotton pathogen, Pantoea vagans","authors":"Muneeb Anwer ,&nbsp;Muhammad Waqas Azam ,&nbsp;Muhammad Taimoor Shakeel ,&nbsp;Anam Moosa ,&nbsp;Hongwei Zhao ,&nbsp;Muhammad Aslam Farooqi ,&nbsp;Mahmoud Moustafa ,&nbsp;Mohammed O. Alshaharni ,&nbsp;Uthman Algopishi ,&nbsp;Huda Alghamdi ,&nbsp;Ambreen Maqsood ,&nbsp;Muhammad Naveed Aslam","doi":"10.1016/j.pmpp.2025.102723","DOIUrl":"10.1016/j.pmpp.2025.102723","url":null,"abstract":"<div><div>Bacterial blight of cotton is a severe disease that significantly impacts cotton production worldwide. In 2022, cotton (<em>Gossypium</em> spp. plants exhibiting angular leaf spots, necrotic lesions, and blackened stems were observed in fields across Bahawalpur, Punjab, Pakistan. Pathogen isolation from symptomatic plants yielded <em>Pantoea vagans</em> (strains CPVB#1, CPVB#2, and CPVB#3), identified through morphological, biochemical, and molecular analyses. Molecular identification using 16S rRNA sequencing (GenBank Accession Nos. OQ925871, OQ925897, OQ925910) revealed high sequence similarity (99.8 %, 98.59 %, and 97.78 %) to <em>Pantoea vagans</em> reference strain <em>MN661279</em>. Further confirmation was achieved via multilocus sequence analysis (MLA) of <em>fusA</em> (OR184128–OR184130) and <em>leuS</em> (OR184131, OR188816, OR188817) genes, confirming the close genetic relationship. Our research revealed that, for the first time in Pakistan, <em>Pantoea vagans</em> is causing bacterial blight in cotton, according to our knowledge. To control bacterial blight in cotton, the efficacy of salicylic acid (SA) as a sustainable control method was also evaluated under greenhouse conditions. SA treatments at 0.5 mM, 1.0 mM, and 1.5 mM reduced disease severity significantly, with 1.5 mM SA achieving the greatest suppression (82.3 %). In addition to lowering disease severity, SA treatments increased total phenolic content and enhanced the activity of enzymes that are related to defense mechanisms, including CAT, POD, SOD, PAL and PPO with maximum enzyme activity observed four Days post-treatment. These results demonstrate the potential of SA as an eco-friendly and effective management strategy for bacterial blight in cotton. Further research is warranted to investigate the epidemiology of <em>Pantoea vagans</em> and develop integrated approaches to mitigate its impact on cotton production.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102723"},"PeriodicalIF":2.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced secondary metabolite accumulation in tomato roots mediated by colonization of Paenibacillus dendritiformis confers crop protection against foot rot caused by Rhizoctonia solani","authors":"Pandian Kirupanatha-Rajan ,&nbsp;Kanagaraj Muthu-Pandian Chanthini ,&nbsp;Rajaseharan Deepthi ,&nbsp;Ramar Manju ,&nbsp;Ganesh-Subbaraja Pavithra ,&nbsp;Ponnusamy Murugan ,&nbsp;Arulsoosairaj Deva-Andrews ,&nbsp;Balamuralikrishnan Balasubramanian ,&nbsp;Rajakrishnan Rajagopal ,&nbsp;Sengottayan Senthil-Nathan","doi":"10.1016/j.pmpp.2025.102721","DOIUrl":"10.1016/j.pmpp.2025.102721","url":null,"abstract":"<div><div>Leveraging the dynamic and adaptable ecological environment of the plant rhizosphere, characterized by intense microbe-plant interactions, mediated by plant secondary metabolites, can sustain and enhance plant development. This study evaluates the potential of rhizosphere bacteria to protect tomatoes against foot-rot disease caused by <em>Rhizoctonia solani</em> (MTCC 12232). The optimal spore-forming soil bacterium, <em>Paenibacillus dendritiformis</em> (PD), isolated from the tomato rhizosphere, demonstrated significant antifungal activity, inhibiting fungal growth by 76.94 % and reducing fungal biomass by 83.69 %. Treatment with PD effectively controlled foot-rot disease, lowering its incidence to 16.2 %. Furthermore, bacterial treatments improved tomato plant root establishment, elongation, and phytohormone production, boosting seed germination to 87 %. The study shows that <em>P. dendritiformis</em> can trigger non-specific immune responses in colonized plants, resulting in higher levels of mycolytic glucanase and chitinase enzymes in infected roots, along with increased accumulation of peroxidase and polyphenol oxidase enzymes. These findings highlight the potential of <em>P. dendritiformis</em> as a viable biocontrol agent for environmentally friendly crop protection and production, with future studies aimed at field trials across diverse locations to validate and optimize its efficacy in various agricultural settings.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"139 ","pages":"Article 102721"},"PeriodicalIF":2.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of hormone signaling on defense responses of invasive Sphagneticola trilobata to pathogen and insect herbivore","authors":"Bharani Manoharan ,&nbsp;Shan-Shan Qi ,&nbsp;Georgios Vidalakis ,&nbsp;Ashraf El-kereamy ,&nbsp;Viswanathan Satheesh ,&nbsp;Dinakaran Elango ,&nbsp;Vignesh Dhandapani ,&nbsp;Zhi-Cong Dai ,&nbsp;Dao-Lin Du","doi":"10.1016/j.pmpp.2025.102722","DOIUrl":"10.1016/j.pmpp.2025.102722","url":null,"abstract":"<div><div>Invasive species often dominate new environments and alter ecosystem diversity and function. Two key mechanisms proposed to explain this success are the Enemy Release Hypothesis (ERH), which suggests that invasive species escape their natural enemies, and the Novel Weapons Hypothesis (NWH), which posits that they possess unique biochemical traits that give them a competitive advantage over native species. Here, we tested the induced defense responses in a highly invasive plant, <em>Sphagneticola trilobata,</em> against the pathogenic fungi (<em>Rhizoctonia solani</em>) and a lace bug herbivore (<em>Corythucha marmorata</em>). The endogenous contents of five hormones: Jasmonic acid (JA), Ethylene (ET), Salicylic acid (SA), Abscisic acid (ABA), and Gibberellic acid (GA) after treatment with either pathogen or herbivore showed higher hormone levels against pathogen and lower levels against herbivore. Further, expression analyses with 17 (nine JA and eight ET pathway) genes were tested against each pathogen and herbivore treatment. In particular, ETHYLENE RESPONSE FACTOR (ERF) branch of the JA pathway is induced for necrotrophic pathogen. Whereas, the basic helix-loop-helix (bHLH) protein (MYC2) branch is induced for herbivore. The differential hormone levels and expression between treatments could be due to different mode of infection mechanisms used by the fungus (necrotrophy) and an insect (piercing and sucking). Specifically, <em>S. trilobata</em> enhance defenses in the invaded area, and these defenses differ between responses to a pathogen and herbivore. Furthermore, our study further confirms that <em>S. trilobata</em> do not reduce their investment in defenses due to a lack of enemies. It may be an adaptation mechanism to fine-tune their defense mechanism while encountering multiple stresses during invasion process of <em>S. trilobata</em>, particularly against local natural enemies.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102722"},"PeriodicalIF":2.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene expression of transcripts encoding putative secreted proteins from an oil palm fungal pathogen Ganoderma boninense","authors":"Mui Sie Jee ,&nbsp;Chai-Ling Ho ,&nbsp;Mohd Termizi Yusof ,&nbsp;Sharon Yu Ling Lau ,&nbsp;Frazer Midot ,&nbsp;Mei Lieng Lo ,&nbsp;Mei-Yee Chin ,&nbsp;Lulie Melling","doi":"10.1016/j.pmpp.2025.102715","DOIUrl":"10.1016/j.pmpp.2025.102715","url":null,"abstract":"<div><div><em>Ganoderma boninense</em> is a white rot fungus which causes basal stem rot (BSR) disease in oil palm and economic loss to the oil palm industry in Southeast Asia. The existing solutions for the disease are ineffective and the genetic determinants responsible for the disease occurrence are understudied. This study reported the characterisation of four transcript sequences encoding metalloproteinase (<em>U14090</em>), aspartic protease (<em>U42611</em>), lipase (<em>U56931</em>), and polysaccharide deacetylase (<em>U128397</em>) from <em>G. boninense</em>. The candidate transcripts were cloned and confirmed by sequencing<em>.</em> The gene expression of <em>U56931</em> was up-regulated in mycelial cultures at 21- and 28-days after inoculation (dai) whereas the expression of <em>U42611</em> was down-regulated in mycelial cultures at 28-dai, in comparison to that at 7-dai. Salicylic acid which is involved in plant biotic stress response was shown to be able to down-regulate the gene expression of <em>U56931</em>. The gene expression of <em>U14090</em> was up-regulated in <em>G. boninense</em> in contact with oil palm roots for 24- and 48-h post inoculation (hpi) compared to that in the uninoculated control. The findings of this study may facilitate the design of future functional studies and help to prioritise candidate fungal genes for gene editing.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102715"},"PeriodicalIF":2.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}