Physiological and Molecular Plant Pathology最新文献

{"title":"Molecular characterization of pectin lyase and endopolygalacturonase from Fusarium commune, causing wilting of Lathyrus sativus L.","authors":"Debraj Ghosal,&nbsp;Rituparna Sarkar,&nbsp;Pinki Tikadar,&nbsp;Bejoysekhar Datta","doi":"10.1016/j.pmpp.2025.102897","DOIUrl":"10.1016/j.pmpp.2025.102897","url":null,"abstract":"<div><div><em>Fusarium commune</em> [1] is an emerging fungal pathogen known to cause severe plant diseases, with its pathogenicity largely attributed to cell-wall-degrading enzymes. This study presents the first report on the molecular characterization, 2D and 3D structural prediction, and validation of two key pectinolytic enzymes, pectin lyase and endopolygalacturonase, produced by <em>F. commune</em>. The genes encoding these enzymes were identified and analyzed using bioinformatics tools to predict their physicochemical properties, domain architecture, and functional motifs. Secondary and tertiary structural models were generated and validated to assess their stability. The molecular modeling revealed conserved catalytic residues essential for enzymatic function, suggesting their involvement in host tissue degradation. These enzymes likely play a significant role in the virulence of <em>F. commune</em> by facilitating host penetration and colonization through pectin degradation. Understanding their structural and functional properties provides insights into their role in fungal pathogenicity. The findings of this study contribute to a deeper understanding of fungal infection mechanisms and offer potential targets for disease management strategies.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102897"},"PeriodicalIF":3.3,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and antifungal potential of Clonostachys rosea: Insights from molecular, metabolomic, and functional analysis","authors":"Shajith Basha Jaffer ,&nbsp;Parthiban V. Kumaresan ,&nbsp;Johnson Iruthayasamy ,&nbsp;Sheneka Rajendran ,&nbsp;Priyank Hanuman Mahtre ,&nbsp;Jayakanthan Mannu ,&nbsp;Anandham Rangasamy ,&nbsp;Elaiyabharathi Thiyagarajan ,&nbsp;Karthikeyan Muthusamy","doi":"10.1016/j.pmpp.2025.102895","DOIUrl":"10.1016/j.pmpp.2025.102895","url":null,"abstract":"<div><div>The present study comprehensively investigates the genetic diversity, antifungal potential, and metabolic profiling of <em>Clonostachys rosea</em> isolates. Genetic variability analysis using Inter Simple Sequence Repeat (ISSR) and Universal Primed PCR (UP-PCR) markers revealed high polymorphism among fifteen <em>C. rosea</em> isolates. ISSR analysis revealed 73.13 % polymorphism, grouping the isolates into three major clusters, whereas UP-PCR showed 72.06 % polymorphism with two distinct clusters. <em>C. rosea</em> CR 02 as the most effective isolate, demonstrating strong antagonistic activity against <em>Rhizoctonia solani</em> PTRhS – 01 under <em>in vitro</em> assays. SEM analysis confirmed mycoparasitism, showing hyphal disintegration and conidial colonization over hyphae. Physiological studies determined the optimal growth conditions for CR 02, with temperature, pH, carbon, and nitrogen sources for enhanced sporulation. Secondary metabolite profiling through Gas Chromatography-Mass Spectrometry (GC-MS) revealed fifteen distinct bioactive compounds from a ditrophic interaction. Heat map and principal component analysis elucidated metabolite distribution patterns. Docking studies revealed strong binding affinities with target proteins, indicating their role in antifungal activity. Overall, <em>C. rosea</em> CR 02 showed strong biocontrol potential, optimal growth, metabolite production, and molecular interactions, making it a promising biocontrol agent.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102895"},"PeriodicalIF":3.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Papaya ringspot virus type P affects photochemical efficiency (JIP test parameters) in young papaya plants","authors":"Catiane dos Santos Braga ,&nbsp;Helaine Christine Cancela Ramos ,&nbsp;Juliana Saltires Santos ,&nbsp;Guilherme Augusto Rodrigues de Souza ,&nbsp;Alex Souza Rodrigues ,&nbsp;Messias Gonzaga Pereira ,&nbsp;Eliemar Campostrini","doi":"10.1016/j.pmpp.2025.102894","DOIUrl":"10.1016/j.pmpp.2025.102894","url":null,"abstract":"<div><div>Papaya ringspot virus type P (PRSV-P) is the causal agent of one of the most devastating viral diseases affecting papaya crops, with symptoms including mosaic and chlorosis. Although understanding of the photochemical changes in infected plants is limited, research such as the present study aims to bridge this gap by investigating how PRSV-P affects the physiological aspects of papaya plants. In this study, chlorophyll fluorescence variables, measured by the JIP test, are used to analyze these changes in ten papaya accessions. The fluorescence variables were measured five days before and 7, 16, 30 and 64 days after inoculation (DAI). Nine of the ten accessions studied showed severe symptoms of the disease and high percentage disease indices (PDIs). These accessions exhibited significant changes in photochemical variables at 30 DAI, including declines in initial and maximum fluorescence and phenomenological fluxes. In addition, relative variable fluorescence in stage I (V_I) increased from 16 DAI onwards. Changes in these variables indicate damage to photosynthetic pigments and the electron transport chain, and inactivation of PSII reaction centers. By contrast, papaya accession BAG89 exhibited resistance to PRSV-P, with a symptom incubation period of 56 days and PDI of only 1.67 %. This accession maintained more stable photochemical efficiency under viral infection. These findings help understand the photochemical response of papaya to viral infection and highlight the potential of BAG89 as a genetic resource in developing resistant varieties, contributing to sustainable management of this virus in cultivation areas.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102894"},"PeriodicalIF":3.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Alternaria tenuissima causing leaf blight disease on Madhuca longifolia (Mahua) seedlings in India","authors":"K. Darshan ,&nbsp;Charishma Krishnappa ,&nbsp;Fatima Shirin ,&nbsp;S.N. Mishra ,&nbsp;Sourabh Dubey ,&nbsp;Dolly Parte ,&nbsp;H.M. Akshay Kumar","doi":"10.1016/j.pmpp.2025.102896","DOIUrl":"10.1016/j.pmpp.2025.102896","url":null,"abstract":"<div><div>Forest tree diseases are often neglected because of their perennial nature and regenerative capacity. One such important tree is <em>Madhuca longifolia,</em> commonly known as Mahua. It is a socioeconomically important deciduous tree species found in India. 12-month-old seedlings showed severe leaf spot and blight symptoms, with 45–70 % disease severity in Ahmadpur Nursery, Bhopal, Madhya Pradesh, during a disease survey in December 2023 and June 2024. Leaf blight disease was extensive, leading to premature defoliation and seedling death in the nursery. The pathogen was isolated and identified as <em>Alternaria tenuissima</em> using a morpho-molecular approach. Multigene sequence analysis of three housekeeping genes, Internal Transcribed Spacer (ITS), Translation Elongation Factor 1-alpha (<em>tef-1α</em>), and major allergen (<em>Alt a1</em>) gene confirmed the identity of the pathogen at the species level. Pathogenicity was established using Koch's postulates, and partial sequences were submitted to NCBI GenBank. Phylogenetic analysis of the concatenated sequences of the three genes revealed a close association between isolate MA1 and the NCBI reference genome of <em>A. tenuissima</em> strain JgmB 36–2<em>.</em> These findings highlight the importance of early detection and management to prevent the spread of this pathogen in forest nurseries and plantations. This is the first report of Mahua leaf blight caused by <em>A. tenuisima</em> in India.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102896"},"PeriodicalIF":3.3,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144894906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling the bioactive metabolites of endophytic Bacillus involved in antagonistic interaction with the Fusarium pathogen of cowpea","authors":"S.J. Sreeja ,&nbsp;M. Siva ,&nbsp;E. Santhoshinii ,&nbsp;K. Saravanakumari ,&nbsp;K.N. Anith","doi":"10.1016/j.pmpp.2025.102879","DOIUrl":"10.1016/j.pmpp.2025.102879","url":null,"abstract":"<div><div><em>Fusarium</em> wilt, caused by <em>Fusarium oxysporum</em> f. sp. <em>tracheiphilum</em> is a devastating disease that affects cowpea globally. Due to limitations and environmental concerns connected with the use of chemical fungicides, sustainable and eco-friendly alternatives, especially biocontrol with microbial agents have gained attention. Antagonistic bacterial endophytes that produce bioactive secondary metabolites can suppress pathogens, promote plant growth and induce systemic resistance, making them ideal choices for integrated disease management strategies. In this study, seventeen out of 38 endophytic bacteria isolated from cowpea demonstrated suppressive effect on the wilt pathogen. <em>Bacillus velezensis</em> CBRE5 and <em>Bacillus amyloliquefaciens</em> CBSE5 showed the highest mycelial growth inhibition. <em>In vivo</em>, their combined application significantly reduced wilt incidence and enhanced plant growth by improving yield. These two isolates were selected for GC-MS analysis to identify key metabolites responsible for their growth-promoting and biocontrol effects. A wide range of compounds belonging to diverse classes were identified. Bioactive metabolites such as 3-methyl-2-phenylindole; benzaldehyde, 2-chloro-3,4-dibenzyloxy; 1H-1,2,3-triazole-4,5-dimethanol,1-(phenylmethyl); and carbamic acid, N-(4-acetylphenyl), (3-tetrahydrothienyl) ester were recognized as potential antifungal agents. Docking studies further highlighted the interactions among these metabolites and four virulent protein targets of the pathogen. Among these, elongation factor 1-alpha exhibited the strongest interaction with 3-methyl-2-phenylindole (binding affinity −7.7 kcal/mol), followed by galacturonan 1,4-alpha-galacturonidase (binding affinity −7.4 kcal/mol). Benzaldehyde (2-chloro-3,4-dibenzyloxy) also displayed dual functional potential, effectively targeting elongation factor 1-alpha and endo-polygalacturonase. These <em>in silico</em> results are preliminary and serve to generate hypotheses about possible mechanisms of action of endophytic <em>Bacillus</em>. The findings support the biocontrol potential of endophytic <em>Bacillus</em> spp., providing a basis for their future application as eco-friendly agents in managing <em>Fusarium</em> wilt of cowpea.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102879"},"PeriodicalIF":3.3,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of blackgram resistance to powdery mildew: Morphological, physiological and biochemical perspectives","authors":"V. Vasanthi ,&nbsp;P. Kishore Varma ,&nbsp;M. Suresh ,&nbsp;N. Kamakshi ,&nbsp;Ch Rani ,&nbsp;D. Ramesh ,&nbsp;K.K. Chetan ,&nbsp;A. Bavana Keerthi ,&nbsp;A. Janaki Prasad ,&nbsp;B. Pushpa ,&nbsp;J. Vasanthi","doi":"10.1016/j.pmpp.2025.102883","DOIUrl":"10.1016/j.pmpp.2025.102883","url":null,"abstract":"<div><div>Powdery mildew poses a significant threat to blackgram (<em>Vigna mungo</em> L.) productivity, demanding sustainable solutions rooted in host resistance. In this study, 121 blackgram genotypes were initially field-screened to capture the spectrum of disease response. From this pool, seventeen genotypes ranging from moderately resistant to highly susceptible were subjected to detailed morphological and physiological assessments. Among them, eight representative genotypes (four moderately resistant and four highly susceptible) were further analyzed for key biochemical and enzymatic responses, revealing distinct defense-associated profiles. Moderately resistant lines demonstrated superior surface wax deposition (TBG-138: 140.33 mg dm⁻²; LBG-648: 108.00 mg dm⁻²), higher trichome density (LBG-648: 27.33 per 5 mm²) and lower stomatal frequency (PBG-23: 76.53 mm⁻²; TBG-138: 102.04 mm⁻²) traits, likely restricting pathogen entry. Biochemically, resistant genotypes exhibited elevated phenol levels, while susceptible ones accumulated more sugars, potentially favoring fungal colonization. Enzymatic assays highlighted intensified activity of key defense enzymes like peroxidase (POD), polyphenol oxidase (PPO) and phenylalanine ammonia-lyase (PAL) in resistant lines, pointing to a stronger immune response. Multivariate analysis reinforced these associations like surface wax and enzymatic defense were negatively correlated with disease severity, while stomatal frequency and sugar content showed positive correlations. Collectively, this integrative study identifies pivotal traits conferring resistance and lays a foundation for breeding resilient blackgram varieties, aligning with the principles of sustainable and eco-conscious agriculture.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102883"},"PeriodicalIF":3.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the invisible threat: cutting-edge methods for detecting fungal and oomycete plant pathogens and mycotoxins","authors":"Rossana Parlascino ,&nbsp;Sebastiano Conti Taguali ,&nbsp;Mario Riolo ,&nbsp;Moricca Salvatore ,&nbsp;József Bakonyi ,&nbsp;David Ezra ,&nbsp;Alessandra Benigno ,&nbsp;Federico La Spada ,&nbsp;Antonella Pane ,&nbsp;Santa Olga Cacciola","doi":"10.1016/j.pmpp.2025.102893","DOIUrl":"10.1016/j.pmpp.2025.102893","url":null,"abstract":"<div><div>Early detection of fungal and oomycete pathogens and accurate monitoring of mycotoxins are critical for ensuring food safety and sustaining agricultural productivity. This review provides a critical overview of current diagnostic tools, covering traditional morphological assessments, serological assays, and advanced molecular approaches such as PCR variants, isothermal amplification (LAMP, RPA), DNA hybridization, and next-generation sequencing. Analytical strategies for mycotoxin detection are also described, including solvent-based extraction, immunoassays, and liquid chromatography coupled with mass spectrometry, alongside emerging biosensor technologies. A distinctive contribution of this review is in the comparative assessment of these methodologies based on shared performance criteria sensitivity, specificity, turnaround time, field applicability, and cost, offering a transversal framework for method selection across phytosanitary contexts. Special attention is given to quarantine pathogens and mycotoxigenic fungi affecting the citrus supply chain, where early and accurate diagnosis is pivotal to prevent yield losses and trade restrictions. The review highlights that method selection should be guided by the specific diagnostic objective, available resources, and regulatory requirements, with integrated strategies often representing the most practical solution to ensure both food safety and phytosanitary security.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102893"},"PeriodicalIF":3.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype-dependent stratification of tomato for Sclerotium rolfsii resistance using physiological, biochemical, and pathogenicity indices","authors":"Muhammad Jarrar Ahmed,&nbsp;Amna Shoaib","doi":"10.1016/j.pmpp.2025.102890","DOIUrl":"10.1016/j.pmpp.2025.102890","url":null,"abstract":"<div><div><em>Sclerotium rolfsii</em> is a disastrous soil-borne pathogen causing stem rot in tomato (<em>Solanum lycopersicum</em>), leading to acute yield losses under warm and humid conditions. This study aimed to characterize <em>S. rolfsii</em> and systematically evaluate the resistance potential of twenty tomato genotypes through integrated morphological, physiological, biochemical, and pathogenicity-based assays. Genotypes were assessed under greenhouse conditions for disease incidence, cumulative severity index, and shoot–root growth. Five genotypes (e.g., Nadir, AUT-309) were categorized as highly resistant, while two genotypes Nagina and Rio Grande, exhibited high susceptibility (DI = 65–75 %) and severe growth suppression (40–60 %). Resistant genotypes retained photosynthetic pigment levels and displayed significant increases in phenolics (up to 71 %) and antioxidant enzymes (PPO up to 200 %, POX up to 80 %, CAT up to 70 %). In contrast, susceptible genotypes exhibited lessened biochemical responses and pigment degradation. Detached leaf and fruit assays supported these trends, with higher necrosis and fungal colonization in susceptible genotypes. Correlation and cluster analyses revealed strong positive associations among PPO, POX, PHE, and TPC in resistant groups, establishing these as main resistance indicators. This integrative assessment provides critical insights into genotype-dependent defense strategies and highlights candidate lines for breeding durable collar rot resistance.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102890"},"PeriodicalIF":3.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144867417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytocytokines: Key regulators of plant immunity and emerging tools for sustainable agriculture","authors":"Bader Alsubaie ,&nbsp;Mohamed Abdel-Haleem ,&nbsp;Fatmah Ahmed Safhi ,&nbsp;Alzhraa Ali Mohamed ,&nbsp;Othman Al-Dossary ,&nbsp;Jameel M. Al-Khayri ,&nbsp;Mustafa I. Almaghasla ,&nbsp;Amira A. Ibrahim","doi":"10.1016/j.pmpp.2025.102889","DOIUrl":"10.1016/j.pmpp.2025.102889","url":null,"abstract":"<div><div>In plant immunity and stress adaptation, phytocytokines, which are endogenous peptide signals, play a central role in regulating immunological responses. These molecules, functionally similar to animal cytokines, belong to several families, including systemins, phytoelicitor peptides (Peps), and rapid alkalinization factors (RALFs). They act through membrane-bound receptor kinases to coordinate complex signaling cascades. Phytocytokines are essential for both local and systemic immunity. They trigger calcium influx, activate defense-related gene expression, and initiate mitogen-activated protein kinase (MAPK) cascades in response to stress or pathogen attack. This study outlines the molecular functions of major phytocytokines, their hormonal crosstalk with the classical phytohormones salicylic acid (SA), jasmonic acid (JA), and ethylene (ET), which are discussed in relation to phytocytokine signaling and their integration into broader plant immune networks. We also highlight their roles in abiotic stress responses, including salinity and drought, supported by recent physiological and molecular data. Biotechnology strategies such as foliar peptide application, transgenic overexpression, and CRISPR/Cas9-mediated genome editing have been explored as tools to increase crop resilience. Case studies have demonstrated that modifying phytocytokine pathways can increase disease resistance, improve resource efficiency, and promote sustainable farming practices. Despite promising advances, challenges remain in terms of scalability, delivery methods, and signaling complexity. Finally, we discuss the future potential of integrating phytocytokines with omics technologies and gene editing to develop high-yielding, climate-resilient crops.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102889"},"PeriodicalIF":3.3,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of Meloidogyne luci using KASP assay","authors":"Zübeyir Devran ,&nbsp;Atilla Göknur","doi":"10.1016/j.pmpp.2025.102888","DOIUrl":"10.1016/j.pmpp.2025.102888","url":null,"abstract":"<div><div><em>Meloidogyne luci</em> is one of the most important root-knot nematodes (RKNs) attacking crops in many parts of the world. It was identified as an emerging nematode species and included in the EPPO Alert List in 2017. Rapid and accurate identification of <em>M. luci</em> is required for management practices and laboratory applications. In the present study, a high-throughput Kompetitive Allele-Specific PCR (KASP) assay was developed for molecular identification of <em>M. luci</em>. The assay easily separated <em>M. luci</em> from the closely related species and other RKN species. This is the first report on the identification of <em>M. luc</em>i using a KASP assay. The KASP primers designed for this study can be used for rapid and accurate identification of <em>M. luci</em>.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"140 ","pages":"Article 102888"},"PeriodicalIF":3.3,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}