用KASP法鉴定露丝花霉的分子结构

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-08-16 DOI:10.1016/j.pmpp.2025.102888

Zübeyir Devran , Atilla Göknur

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引用次数: 0

摘要

绿根结线虫（Meloidogyne luci）是世界上许多地区危害农作物最重要的根结线虫之一。它被确定为一种新兴的线虫物种，并于2017年被列入EPPO警报清单。快速准确地鉴定露西芽孢杆菌是管理实践和实验室应用所必需的。本研究建立了一种高通量竞争等位基因特异性PCR （KASP）方法对luci进行分子鉴定。该方法能很容易地将luci与近缘种及其它RKN种分离开来。这是首次用KASP法鉴定露西分枝杆菌。本研究设计的KASP引物可用于快速、准确地鉴定绿僵菌。

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Molecular identification of Meloidogyne luci using KASP assay

Meloidogyne luci is one of the most important root-knot nematodes (RKNs) attacking crops in many parts of the world. It was identified as an emerging nematode species and included in the EPPO Alert List in 2017. Rapid and accurate identification of M. luci is required for management practices and laboratory applications. In the present study, a high-throughput Kompetitive Allele-Specific PCR (KASP) assay was developed for molecular identification of M. luci. The assay easily separated M. luci from the closely related species and other RKN species. This is the first report on the identification of M. luci using a KASP assay. The KASP primers designed for this study can be used for rapid and accurate identification of M. luci.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.