蔷薇的遗传多样性和抗真菌潜力：来自分子、代谢组学和功能分析的见解

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-08-20 DOI:10.1016/j.pmpp.2025.102895

Shajith Basha Jaffer , Parthiban V. Kumaresan , Johnson Iruthayasamy , Sheneka Rajendran , Priyank Hanuman Mahtre , Jayakanthan Mannu , Anandham Rangasamy , Elaiyabharathi Thiyagarajan , Karthikeyan Muthusamy

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引用次数: 0

摘要

本研究全面研究了蔷薇枝孢杆菌分离株的遗传多样性、抗真菌潜力和代谢谱。利用ISSR和UP-PCR对15株玫瑰念珠菌进行遗传变异分析，发现菌株具有较高的多态性。ISSR多态性为73.13%，可划分为3个主要聚类；UP-PCR多态性为72.06%，可划分为2个不同的聚类。在体外实验中，对茄枯丝核菌PTRhS - 01表现出较强的拮抗活性。扫描电镜分析证实了真菌寄生，显示菌丝解体和分生孢子在菌丝上定植。生理学研究确定了cr02的最佳生长条件，温度、pH、碳和氮源都能促进孢子的形成。二级代谢物分析通过气相色谱-质谱（GC-MS）揭示了15种不同的生物活性化合物来自二营养相互作用。热图和主成分分析阐明了代谢物的分布模式。对接研究显示其与靶蛋白具有很强的结合亲和力，表明其具有抗真菌活性。总体而言，红毛念珠菌cr02表现出较强的生物防治潜力、最佳的生长、代谢产物产生和分子相互作用，是一种很有前景的生物防治剂。

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Genetic diversity and antifungal potential of Clonostachys rosea: Insights from molecular, metabolomic, and functional analysis

The present study comprehensively investigates the genetic diversity, antifungal potential, and metabolic profiling of Clonostachys rosea isolates. Genetic variability analysis using Inter Simple Sequence Repeat (ISSR) and Universal Primed PCR (UP-PCR) markers revealed high polymorphism among fifteen C. rosea isolates. ISSR analysis revealed 73.13 % polymorphism, grouping the isolates into three major clusters, whereas UP-PCR showed 72.06 % polymorphism with two distinct clusters. C. rosea CR 02 as the most effective isolate, demonstrating strong antagonistic activity against Rhizoctonia solani PTRhS – 01 under in vitro assays. SEM analysis confirmed mycoparasitism, showing hyphal disintegration and conidial colonization over hyphae. Physiological studies determined the optimal growth conditions for CR 02, with temperature, pH, carbon, and nitrogen sources for enhanced sporulation. Secondary metabolite profiling through Gas Chromatography-Mass Spectrometry (GC-MS) revealed fifteen distinct bioactive compounds from a ditrophic interaction. Heat map and principal component analysis elucidated metabolite distribution patterns. Docking studies revealed strong binding affinities with target proteins, indicating their role in antifungal activity. Overall, C. rosea CR 02 showed strong biocontrol potential, optimal growth, metabolite production, and molecular interactions, making it a promising biocontrol agent.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.