Physiological and Molecular Plant Pathology最新文献

{"title":"Resistance mechanisms in Oryza species against Meloidogyne graminicola: Insights into nematode penetration and reproduction","authors":"M. Themuhi ,&nbsp;Debanand Das ,&nbsp;B. Rajeshwaran ,&nbsp;Mateti Gayithri ,&nbsp;K. Narmadha","doi":"10.1016/j.pmpp.2025.102733","DOIUrl":"10.1016/j.pmpp.2025.102733","url":null,"abstract":"<div><div>The present study was conducted to understand the resistance mechanisms of various <em>Oryza</em> species against <em>Meloidogyne graminicola</em>, focusing on nematode penetration, development, reproduction, and biochemical responses. Resistant (<em>Oryza glaberrima</em>: IR 101800, IR 102226 and IR 102336), moderately resistant (<em>O. rufipogon</em> (local germplasm) and <em>O. sativa</em>: var. Sonjul Bao), and susceptible (<em>O. sativa</em>: var. Luit and Bina Dhan 11) germplasm/varieties were considered for these studies. Nematode penetration was significantly delayed in resistant germplasm, initiating at 4 days after inoculation (DAI), compared to 12 h in susceptible varieties. At 28 DAI, resistant germplasm shelter fewer juveniles and produced fewer galls (6–9/plant); compared to the susceptible rice variety Luit (22 galls/plant). The phenolic compound accumulation was increased resulting in enhanced defence enzyme activity in resistant germplasm. Total phenol content peaked at 14 DAI, with <em>O. glaberrima</em> (IR 102226) showing the highest levels (153.1 μg GA/g fresh weight) compared to Luit (87.6 μg GA/g). Enzymatic activity was elevated in resistant germplasm, with IR 102226 recording 8.8 U/g peroxidase (PO), 72.4 U/g polyphenol oxidase (PPO), and 120.8 U/g phenylalanine ammonia lyase (PAL) at 14 DAI, significantly outperforming the susceptible check. Principal component analysis demonstrated strong correlations between elevated phenolic content, enzyme activity, and nematode resistance. These findings underscore the pivotal role of biochemical defences in delaying nematode development and reproduction in resistant germplasm. <em>O. glaberrima</em> (IR 102226) exhibiting robust pre- and post-infectional resistance is a valuable genetic resource for breeding programs targeting sustainable <em>M. graminicola</em> management.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102733"},"PeriodicalIF":2.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel report of Rhizoctonia solani AG-4 HG-I associated with damping-off and wilt of quinoa (Chenopodium quinoa) in India","authors":"D. Chethan ,&nbsp;R. Chandana ,&nbsp;Farooq Khan ,&nbsp;B.S. Kavya ,&nbsp;T. Tharana Poonacha ,&nbsp;R. Karan ,&nbsp;Akash Bevanur ,&nbsp;R. Kruthika ,&nbsp;K.S. Ashwini ,&nbsp;Yerranagari Vani ,&nbsp;S.R. Anand ,&nbsp;A. Mohan Rao ,&nbsp;K.B. Palanna","doi":"10.1016/j.pmpp.2025.102725","DOIUrl":"10.1016/j.pmpp.2025.102725","url":null,"abstract":"<div><div>Quinoa, <em>Chenopodium quinoa</em>, was domesticated first in the Andean countries of South America and has been widely cultivated in most countries due to its unique nutritional value and health benefits. In fields, characteristic wilting and girdling symptoms on stems of quinoa plants were observed in Bengaluru and Chamarajanagara districts, Karnataka, India. The associated pathogen was isolated, purified and identified as <em>Rhizoctonia</em> sp. based on cultural and morphological characterization and nuclear staining revealed a multi-nuclei condition of the pathogen. Molecular and phylogenetic analysis with internal transcribed spacer (ITS) region supported by cultural morphological characteristics revealed <em>Rhizoctonia solani</em> as a causal agent and its anastomosis group AG-4 HG-I confirmed using specific primers for each anastomosis group and sub-group. A pathogenicity test combined with pathogen re-isolation and identification was carried out to validate Koch's postulates. Cultural characterization revealed that potato dextrose agar (PDA) at 30 °C with pH 7 was ideal for better growth of <em>R</em>. <em>solani</em>. To the best of our knowledge, this is the first report of damping-off and wilt caused by <em>R</em>. <em>solani</em> on <em>C</em>. <em>quinoa</em> in India.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102725"},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multigene phylogeny and diversity of Phytophthora and Phytopythium species associated with avocado root rot in India and development of a point-of-care LAMP assay for Phytophthora cinnamomi and Phytopythium vexans","authors":"G.S. Madhu ,&nbsp;A.T. Rani ,&nbsp;B.M. Muralidhara ,&nbsp;G. Nayan Deepak ,&nbsp;S. Rajendiran ,&nbsp;M. Ayyandurai ,&nbsp;V. Venkataravanappa","doi":"10.1016/j.pmpp.2025.102735","DOIUrl":"10.1016/j.pmpp.2025.102735","url":null,"abstract":"<div><div>Avocado root rot, predominantly caused by <em>Phytophthora cinnamomi</em> poses a major threat to avocado cultivation worldwide. However, the potential role of other <em>Phytophthora</em> and <em>Phytophthora</em>-like oomycete species in avocado decline remains largely unexplored. To address this knowledge gap, surveys were conducted across avocado orchards in the Western Ghats region of Karnataka, Kerala, and Tamil Nadu, India, to assess the diversity and pathogenicity of <em>Phytophthora</em> and <em>Phytopythium</em> species associated with root rot. Oomycete isolates were recovered from symptomatic root samples collected from 42 avocado orchards. Morphological characterization, coupled with multilocus sequence analysis targeting the internal transcribed spacer (ITS) region, cytochrome <em>c</em> oxidase subunit 1 (<em>COX1</em>), and β-tubulin (<em>β-tub</em>) genes, facilitated species identification. Furthermore, pathogenicity tests confirmed the virulence of the isolates. Among the surveyed orchards, <em>Phytophthora</em> species were isolated from 26 orchards, with <em>P. cinnamomi</em> being the most frequently detected, occurring in 22 orchards. Additionally, <em>P. tropicalis</em> was identified in 2 orchards, while <em>P. nicotianae</em> and <em>P. kelmanii</em> were each found in 1 orchard. In parallel, 9 <em>Phytopythium</em> isolates were recovered, with <em>Phytopythium vexans</em> detected in 7 orchards and <em>Phytopythium chamaehyphon</em> in 2 orchards. Among all isolates, <em>P. cinnamomi</em> exhibited the highest virulence, underscoring its primary role in avocado root rot. To facilitate early and accurate detection of the predominant pathogens, <em>Phytophthora cinnamomi</em> and <em>Phytopythium vexans</em>, in infected plants and nursery seedlings, a loop-mediated isothermal amplification (LAMP) assay was developed. The sensitivity of LAMP assay detection limits for <em>P. cinnamomi</em> was 25 pg, while for <em>Pp. vexans</em> was detected at 50 pg. This assay provides a rapid, highly sensitive, and reliable diagnostic tool, strengthening disease surveillance and management strategies for avocado cultivation in the region. This study highlighted the novel host record of <em>P. Kelmanii</em> and <em>Pp. chamaehyphon</em> in avocado root rot and <em>Pp. vexans</em> first report in India.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102735"},"PeriodicalIF":2.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Colletotrichum nymphaeae inciting crown rot in strawberry from India: Insights from morpho-cultural characteristics and multi-gene phylogenetics","authors":"Adesh Kumar ,&nbsp;Shubham Saini ,&nbsp;Anil Kumar Saini ,&nbsp;Sushil Sharma ,&nbsp;Rakesh Gehlot ,&nbsp;Anil Kumar ,&nbsp;Rakesh Kumar ,&nbsp;K.C. Rajeshkumar ,&nbsp;Roomi Rawal ,&nbsp;Vikas Kumar Sharma ,&nbsp;R.P.S. Dalal","doi":"10.1016/j.pmpp.2025.102731","DOIUrl":"10.1016/j.pmpp.2025.102731","url":null,"abstract":"<div><div>Crown rot is a devastating disease of strawberry (<em>Fragaria</em> x <em>ananassa)</em> leading to substantial yield losses across world. Further it is a complex disease with involvement of multiple pathogens as incitant worldwide, making it challenging to rule out the exact causal agent. During a recent survey of strawberry farm in Haryana during <em>November</em> 2024, severe crown rot symptoms were observed on crown only whilst no symptoms were observed on fruit and leaves at farmer's field. Morpho-cultural characterization revealed the association of species from <em>Colletotrichum acutatum</em> species complex. Multi-gene phylogenetics based on internal transcribed spacer (ITS), <em>actin</em> (<em>ACT</em>), <em>glyceraldehyde-3-phosphate dehydrogenase</em> (<em>GAPDH</em>) confirmed the identity of pathogen as <em>Colletotrichum nymphaeae</em>. Pathogenicity tests revealed that in addition to crown region, the pathogen have ability to infect leaves and fruits. To our knowledge, this is the first report of <em>Colletotrichum nymphaeae</em> inciting strawberry crown rot in India and it underscores the need for heightened vigilance and integrated disease management strategies to mitigate its potential threat to strawberry production.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102731"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of endophytes against Rhizoctonia bataticola: Antifungal activity, metabolite profiling, and plant defense mechanisms","authors":"Aarthi R ,&nbsp;Devanathan M ,&nbsp;Harish S ,&nbsp;Ganesan K N ,&nbsp;Manivannan V","doi":"10.1016/j.pmpp.2025.102734","DOIUrl":"10.1016/j.pmpp.2025.102734","url":null,"abstract":"<div><div>Groundnut (<em>Arachis hypogaea</em> L.), a vital oilseed crop, is highly vulnerable to root rot disease caused by <em>Rhizoctonia bataticola</em> (Taub.) Butler. This study focused on isolating endophytes with antagonistic potential against <em>R. bataticola</em>. Among the isolated endophytes, <em>Bacillus subtilis</em> RMV 3 exhibited the highest inhibition rate (83.33 %) in culture filtrate assays, followed by <em>Trichoderma asperellum</em> RTM 6 (71.85 %). Gas chromatography-mass spectrometry (GC-MS) analysis of secondary metabolites revealed notable compounds, including Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-, which demonstrated strong molecular binding affinities of −7.1 kcal/mol against chitinase and −6.9 kcal/mol against transferase. Under greenhouse conditions, seed treatment and soil application with <em>T. asperellum</em> RTM 6 and <em>B. subtilis</em> RMV 3 significantly improved plant performance, enhancing the vigour index (5613.58) and germination rate (91.23 %), while reducing disease incidence to just 6.7 %. In the health check, the germination rate was (75.32 %) with vigour index (2872.46). Additionally, these treatments induced a pronounced increase in defense-related enzyme activities, including peroxidase (3.49fold increase), polyphenol oxidase (4.73fold increase) and phenylalanine ammonia-lyase (1.88fold increase) over control in groundnut plants. These findings highlight the potential of <em>T. asperellum</em> RTM 6 and <em>B. subtilis</em> RMV 3 as effective biological control agents, offering a sustainable approach to managing root rot in groundnut cultivation.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102734"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant's cellular response to biotic stress: A review","authors":"Sandeep Kumar Chaurasia ,&nbsp;Chinmayee Acharya ,&nbsp;Jitendra Kumar Pandey","doi":"10.1016/j.pmpp.2025.102718","DOIUrl":"10.1016/j.pmpp.2025.102718","url":null,"abstract":"<div><div>Biotic stress is a severe environmental constraint to the plant's health, ecological balance, and sustainable crop production. Emerging data indicate global agricultural losses, flustered plant-population ecology, and wavering conservational status due to overexploitation of plant life by other living organisms, specifically viruses, bacteria, fungi, nematodes, herbivores, and weeds. In such a setting, plants under biotic stress elusively undergo various biochemical, physiological, and ecological changes for their survival. Plants employ sophisticated mechanisms at different levels with variable adeptness to minimize metabolic constraints and compensate for multidimensional effects of biotic stress. In this review, we have attempted to traverse different types of phytopathogens, mechanisms of their action, diseases and their symptoms. Stress-signal perception, the role of inter-organellar communications, pathogen-triggered immunity, effector-triggered immunity, the role of phytohormones, and secondary metabolites were discussed in detail. At present, we have limited our study to plants' response to biotic stress without the contribution of external assistance. Great advancements have been made in exploring host-pathogen interactions, yet research is still required to elucidate this intricate interaction on molecular, physiological, and ecological levels.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102718"},"PeriodicalIF":2.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stacking of elite indica rice genotype with bacterial blight, blast, and Brown plant hopper resistance genes through marker assisted selection","authors":"Velprabakaran Senthilvel ,&nbsp;Garima Pelhania ,&nbsp;Gokulakrishnan Moorthy ,&nbsp;Yazhini Sukumar ,&nbsp;Priyanka Ariyapalayam Rajendran ,&nbsp;Suji Kalampalayam Krishnaswamy ,&nbsp;Chudamani Sridhar ,&nbsp;Harsa pravena Viswanathan ,&nbsp;Swetha Dhamodaran ,&nbsp;Thirumalraj Samikannu ,&nbsp;Sravanthi Ragulakollu ,&nbsp;Bonipas Antony John ,&nbsp;Niranjanadevi Jeevanandham ,&nbsp;Gopalakrishnan Chellappan ,&nbsp;Sheela Venugopal ,&nbsp;Saraswathi Ramaswamy ,&nbsp;Ramalingam Jegadeesan","doi":"10.1016/j.pmpp.2025.102730","DOIUrl":"10.1016/j.pmpp.2025.102730","url":null,"abstract":"<div><div>Brown planthoppers, bacterial blight, and blast are the predominant biotic stress factors that cause tremendous yield loss in rice. To address these challenges, this study aimed to develop high-yielding rice lines resistant to multiple stresses by incorporating various resistance genes through functional/linked marker-assisted selection. The common rice variety CO51 from India was initially enhanced with the bacterial blight resistance genes <em>xa5</em>, <em>xa13</em>, and <em>Xa21</em>, as well as the blast resistance gene <em>Pi54</em>. The improved CO51 was backcrossed with the donor PTB33 to introgress the BPH resistance genes <em>bph2</em> and <em>Bph32</em>, resulting in the development of F₁ hybrids. Marker-assisted backcrossing was employed to successively backcross these hybrids, resulting in the development of BC₁F₁ and later BC₂F₁ hybrids. Foreground selection <em>via</em> linked markers was performed on F₁, BC₁F₁, and BC₂F₁ hybrids by selecting plants heterozygous for all target genes and assessing their recurrent parent genome (RPG%) levels. In the BC₂F₃ generation, along with foreground selection, phenotypic selection was conducted, and 27 superior plants out of 530 with CO51 characteristics were selected. The selected plants were further subjected to quality trait analysis and phenotypic screening for resistance to bacterial blight, blast, and BPH biotypes. The bioassay confirmed that pyramiding genes or single-gene introgression enhanced resistance compared with that of the recurrent parent, CO51.The six pyramided lines, CB MAS 22059 (BB and blast), CB MAS 22030 and 22042 (BPH and BB) and CB MAS 22009,22015 and 22019 (BPH, BB and blast) are promising with high grain yield, good grain quality traits and multiple stress resistance, these lines could be released as an improved CO51 cultivars for disease prone areas.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102730"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture filtrates derived from endophytes Epicoccum spp. confer resistance against tobacco mosaic virus in Nicotiana benthamiana","authors":"Qi An ,&nbsp;Clement Nzabanita ,&nbsp;Lihang Zhang ,&nbsp;Yanfei Wang ,&nbsp;Shuangchao Wang ,&nbsp;Lihua Guo ,&nbsp;Yan Zhao","doi":"10.1016/j.pmpp.2025.102732","DOIUrl":"10.1016/j.pmpp.2025.102732","url":null,"abstract":"<div><div>Tobacco mosaic virus (TMV) is a plant viral disease significantly affecting the losses of many economically important crops, and no active viricide is currently available for agricultural use. Herein, the efficacy of culture filtrates derived from fungal endophytes <em>Epicoccum layuense</em> strain J4-3 and <em>E. nigrum</em> strain 38L1, respectively isolated from symptomless stem of wheat and leaf of maize, was evaluated in reducing TMV infection in <em>Nicotiana benthamiana</em>. In doing so, dual foliar treatment was carried out 24 h before TMV inoculation under greenhouse conditions. Tracking the spread of green fluorescence protein-tagged TMV (TMV-GFP) in <em>N. benthamiana</em> plants revealed that the culture filtrates restricted the initial establishment of virus infection. Notably, quantitative reverse transcription polymerase chain reaction (qRT-PCR) results indicated a notable decrease in TMV accumulation in plants treated with endophyte's filtrates compared to the untreated controls. Additionally, using liquid chromatography-mass spectrometry (LC-MS), we identified a total of 8366 metabolites, including 569 differential metabolites based on variable importance in projection (VIP) &gt; 1 and <em>P</em> &lt; 0.05. Interestingly, previously reported metabolites with anti-TMV activity namely 13-Docosenamide, Chondroitin sulfate, and Melatonin were identified in culture filtrates of the strains J4-3 and 38L1. This is the first study depicting culture filtrates derived from fungal endophytes <em>Epicoccum</em> spp. to extenuate TMV virulence in <em>N. benthamiana.</em></div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102732"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying fungi responsible for trunk and scaffold diseases in almonds in Türkiye","authors":"Emel Ören ,&nbsp;Harun Bayraktar","doi":"10.1016/j.pmpp.2025.102729","DOIUrl":"10.1016/j.pmpp.2025.102729","url":null,"abstract":"<div><div>A comprehensive survey was conducted between 2020 and 2021 to identify fungal pathogens contributing to almond decline in the Southeastern Anatolia Region of Türkiye. Symptomatic samples were collected from 161 orchards across three provinces, targeting trunks and branches displaying gummosis, stem cankers, branch dieback, wilting, vascular discoloration, and necrosis. In total, 324 samples exhibiting canker symptoms were processed, yielding 196 fungal isolates belonging to 14 species. Morphological characterization and DNA sequence analyses identified <em>Botryosphaeria dothidea, Neoscytalidium dimidiatum, Macrophomina phaseolina, Clonostachys rosea, Fusarium brachygibbosum, F. oxysporum, F. proliferatum, F. redolens, F. solani, F. acuminatum, F. verticillioides, Aspergillus flavus, Paecilomyces maximus,</em> and <em>Verticillium dahliae. F. proliferatum</em> was the dominant species (33.2 % prevalence), followed by <em>V. dahliae, N. dimidiatum,</em> and <em>F. verticillioides.</em> Pathogenicity assays revealed that all fungal species were capable of inducing necrotic lesions at inoculation points on branch segments and seedlings. This study documented the first occurrence of <em>F. verticillioides, P. maximus, C. rosea,</em> and <em>A. flavus</em> on almond, as well as the first reports of <em>F. oxysporum, F. proliferatum, F. redolens, F. acuminatum, F. brachygibbosum,</em> and <em>V. dahliae</em> in association with almond trees in Türkiye.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102729"},"PeriodicalIF":2.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environment disturbances influence bacterial diversity, structure and co-occurrence network in the Digitaria sanguinalis rhizosphere","authors":"Yiming Dai ,&nbsp;Ye Cui ,&nbsp;Qingchen Xiao ,&nbsp;Meiling Ping ,&nbsp;Yiming Zhang ,&nbsp;Shiguang Hu ,&nbsp;Hao Chen ,&nbsp;Xiaoyu Li ,&nbsp;Jin Chen","doi":"10.1016/j.pmpp.2025.102716","DOIUrl":"10.1016/j.pmpp.2025.102716","url":null,"abstract":"<div><div>Physicochemical properties of the soil and vegetation types exert primary influences on soil microorganisms, yet there is limited research on the impacts of two different levels of disturbance, especially in the rhizosphere of the <em>Digitaria sanguinalis</em>. To fill this knowledge gap, we employed the Illumina Miseq high-throughput sequencing technology to obtain 16S rRNA from the rhizosphere in D. sanguinalis soil near and away from the roadside. The rhizosphere soil microorganisms in the two locations experienced two different levels of disturbance and exhibited notable diversity. Meanwhile, there has been a marked rise in the abundance of the Acidobacteria genus <em>RB41</em> within this lightly disturbed region. The analysis of the co-occurrence network suggests that lightly disturbed areas exhibit the presence of two keystone taxa. In contrast to the heavily disturbed site (HD), the lightly disturbed site (LD) exhibits an increased level of the modular network (0.482 vs. 0.448) and stronger competition within the rhizosphere soil microbial community (positive/negative link ratio, P/N: 0.881 vs. 0.864). The findings from the PLS-SM analysis reveal that keystone taxa have the most total effects on aboveground biomass (0.957). These discoveries have important implications for understanding how different levels of disruption impact soil ecosystems.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102716"},"PeriodicalIF":2.8,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143922276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}