Physiological and Molecular Plant Pathology最新文献

{"title":"Outstanding biocontrol and plant growth promotion traits of Pseudomonas fluorescens UM270 and other plant-associated Pseudomonas","authors":"Gustavo Santoyo ,&nbsp;Blanca Rojas-Sánchez ,&nbsp;Julie Hernández-Salmerón ,&nbsp;Rocío Hernández-León ,&nbsp;Daniel Rojas-Solis ,&nbsp;Gabriel Moreno-Hagelsieb ,&nbsp;Ma del Carmen Orozco-Mosqueda","doi":"10.1016/j.pmpp.2025.102672","DOIUrl":"10.1016/j.pmpp.2025.102672","url":null,"abstract":"<div><div>Plant-beneficial <em>Pseudomonas</em> spp. is a group of soil bacteria with a broad metabolic and functional repertoire that has been exploited as bioinoculants to enhance crop health and production. Among them, <em>Pseudomonas fluorescens</em> strain UM270 stands out as a biocontrol agent and plant growth promoter, which was isolated from the rhizosphere of <em>Medicago truncatula</em> plants in Morelia, Mexico. Its genome contains genes with direct and indirect beneficial functions for plants, such as the production of siderophores, 2,4-diacetylphloroglucinol, phosphate solubilization, phenazines, cyanogens, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, proteases, indole-3-acetic acid, or antimicrobial volatiles like dimethyl disulfide and dimethylhexadecylamine, among others. Its antagonistic properties have been tested against major fungal pathogens such as <em>Botrytis cinerea</em>, <em>Rhizoctonia solani</em>, <em>Diaporthe phaseolorum</em>, <em>Fusarium</em> spp., and <em>Colletotrichum lindemuthianum</em>. The UM270 strain has been shown to be beneficial (either in greenhouse or open-field conditions) for crops such as maize, common bean, husk tomato, blueberry, tomato and squash. In this review, we analyze the phylogenetic, genomic, functional and ecological interactions traits of the UM270 strain in the context of other <em>Pseudomonas</em> spp. strains, highlighting its potential as a bioinoculant to address the challenges of sustainable agriculture.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102672"},"PeriodicalIF":2.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of 2-decanone against Monilinia fructicola and its application in combination with boscalid in mitigating brown rot disease in peach fruit","authors":"Xiaorui Wang ,&nbsp;Mingming Huang ,&nbsp;Wenhui Li ,&nbsp;Yingying Shi ,&nbsp;Yinuo Tang ,&nbsp;Han Zhang ,&nbsp;Bin Wu ,&nbsp;Zheng Zhang ,&nbsp;Zunyang Song ,&nbsp;Jingying Shi","doi":"10.1016/j.pmpp.2025.102665","DOIUrl":"10.1016/j.pmpp.2025.102665","url":null,"abstract":"<div><div>Peach fruit is highly susceptible to <em>Monilinia fructicola</em> (<em>M. fructicola</em>), a pathogen that causes significant postharvest losses. Enhancing the efficacy and reducing the dosage of chemical fungicides are critical for controlling brown rot caused by <em>M. fructicola</em>. Natural volatile organic compounds (VOCs) have been proposed as a selective method for managing postharvest pathogen infection. Among these, 2-decanone, a natural volatile produced by fungi and insects, has demonstrated potential in inhibiting pathogen growth. In this study, 60 μL/L 2-decanone effectively inhibited the growth of <em>M. fructicola</em> mycelia. It also reduced spore germination and appressorium formation by downregulating the expression of <em>MfBmp1</em> and <em>MfPls1</em>, respectively. In addition, 2-decanone induced spore apoptosis by increasing reactive oxygen species (ROS) accumulation and exacerbating mitochondrial damage. When applied to peach fruit, 2-decanone fumigation significantly reduced disease incidence and lesion diameter compared to the control group, without adversely affecting fruit quality during storage. Furthermore, we observed that 2-decanone exhibited synergistic effects when combined with boscalid, effectively inhibiting the growth of <em>M. fructicola</em>. Applying boscalid 15 days before harvest and fumigating with 2-decanone postharvest provided similar brown rot suppression as boscalid alone but used only one-tenth the amount of fungicide. Overall, 2-decanone fumigation presents an effective strategy for controlling postharvest brown rot disease in peaches, and its combined use with boscalid introduces a promising approach for enhancing postharvest peach fruit preservation.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102665"},"PeriodicalIF":2.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Neofusicoccum parvum small secreted proteins belonging to Alt a1 and cerato-platanin families affecting defenses in Nicotiana benthamiana and Vitis vinifera","authors":"Alexia Laura Grau ,&nbsp;Romain J.G. Pierron ,&nbsp;Charlotte Doerr ,&nbsp;Hélène Laloue ,&nbsp;Mary-Lorène Goddard ,&nbsp;Julie Chong","doi":"10.1016/j.pmpp.2025.102659","DOIUrl":"10.1016/j.pmpp.2025.102659","url":null,"abstract":"<div><div>Botryosphaeria dieback, which is associated with xylem-inhabiting <em>Botryosphaeriaceae</em> fungi, is one of the major Grapevine Trunk Diseases (GTDs) responsible for significant reduction of longevity and productivity of grapevine. Since the ban of sodium arsenite, no efficient treatment is available to fight against GTDs. <em>Neofusicoccum parvum</em> is considered as one of the most aggressive <em>Botryosphaeriaceae</em> causing wood decay. However, little is known about the molecular players of host-pathogen interaction. In this study, we characterize three small secreted proteins (NpCP1, NpAA1.1, and NpAA1.2) from <em>N. parvum</em>, previously isolated by their affinity for arsenite. Sequence and structure analysis revealed that these secreted proteins belong to cerato-platanin and major allergen Alt a1 families, and have strong structure similarity with known effectors from other pathogenic fungi. <em>NpCP1</em>, <em>NpAA1.1</em>, and <em>NpAA1.2</em> expression was early induced after infection of grapevine wood with <em>N. parvum</em>, suggesting a role in the colonization process. Further investigation of the role of these secreted proteins was realized after transient overexpression in <em>Nicotiana benthamiana</em> and <em>Vitis vinifera</em>. Whereas their expression did not trigger extensive cell death in both systems, our work shows that these small secreted proteins could differentially manipulate the expression of defense genes after agroinfiltration, and could thus be considered as effectors. More specifically, downregulation of enzymes involved in ROS production and ethylene biosynthesis suggests a role during wood colonization.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102659"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-oxygen-voltage photoreceptor-regulated virulence in the pathogenic bacterium Pseudoalteromonas piscicida X-8 of a farmed seaweed bleaching disease","authors":"Lingyuan He ,&nbsp;Yajing Dong ,&nbsp;Mengxin Wang ,&nbsp;Yujiang Wang ,&nbsp;Renjie Wang ,&nbsp;Qin Lin ,&nbsp;Mingyu Ma ,&nbsp;Weifeng Gong ,&nbsp;Gaoge Wang","doi":"10.1016/j.pmpp.2025.102667","DOIUrl":"10.1016/j.pmpp.2025.102667","url":null,"abstract":"<div><div>The outbreaks of bleaching disease of the farmed <em>Saccharina japonica</em> are closely associated with the increased light intensity at the late nursery stage. Based on the light-oxygen-voltage (LOV) photoreceptor in the causative bacterium <em>Pseudoalteromonas piscicida</em> X-8 (PpX-8), we hypothesize that PpX-8 has a light-regulated virulence mechanism through LOV photoreceptors as those in phytopathogenic bacteria. In this study, combining infection assays, bioinformatics approaches, and gene knockout techniques, we investigated the light-regulated virulence mechanism of PpX-8. Our results showed that white light negatively affected the growth but enhanced both motility and virulence of PpX-8 compared to darkness. The light-oxygen-voltage photoreceptor of PpX-8 (PpX-8-LOV) was found to negatively regulate growth and biofilm formation under white light while enhancing motility and virulence at the initial stage of infection. Furthermore, the photoreceptor PpX-8-LOV up-regulated the expression of genes involved in quorum sensing, iron acquisition, and the type II secretion system, thereby enhancing the virulence of PpX-8. This study provides valuable insights for <em>S</em>. <em>japonica</em> farms to optimize light management strategies, thereby effectively preventing or reducing the occurrence of bleaching disease.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102667"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the efficient transmission of cucurbit aphid-borne yellows virus (Polerovirus CABYV) by Aphis gossypii glover in bitter gourd","authors":"Sundaravadivel Sathiya Priya ,&nbsp;Mariappan Suganthy ,&nbsp;Perumal Renukadevi ,&nbsp;Marimuthu Murugan","doi":"10.1016/j.pmpp.2025.102669","DOIUrl":"10.1016/j.pmpp.2025.102669","url":null,"abstract":"<div><div>The Polerovirus cucurbit aphid-borne yellows virus (CABYV) infecting bitter gourd was efficiently transmitted, manifesting in symptoms like leaf cupping, complete yellowing, chlorotic patches, and orange discoloration of young leaves, accompanied by vein thickening. Older leaves exhibited dark green mottling, yellowing along the margins, and a leathery texture. In field conditions, the aphid population (8.95 per plant) and CABYV incidence (85.6 %) were the highest in Coimbatore district of Tamil Nadu, while the greatest yield loss of up to 72.38 % occurred in Erode district. Molecular characterization confirmed the presence of CABYV in 19 out of 23 plant samples collected from various locations in Tamil Nadu through PCR. The aphid species <em>Aphis gossypii</em> was identified via morphological traits and PCR with MtCOI primers. Aphids required a minimum of 30 min for the acquisition access period (AAP) to become viruliferous, resulting in 30 % transmission of CABYV. The inoculation access period (IAP) of 30 min for viruliferous aphids led to 46.67 % transmission, with a gradual increase in transmission percentage as IAP increased. At 7 h (h) of both AAP and IAP, 100 % transmission was observed. A single viruliferous aphid transmitted CABYV in 33.33 % of cases, while a minimum of ten aphids was necessary for complete transmission. Aphids successfully retained and transmitted CABYV for at least five days, with transmission being sporadic. Despite its significance, no previous studies have investigated the virus-vector interaction in CABYV transmission. This study offers the first comprehensive analysis of CABYV transmission dynamics through <em>Aphis gossypii</em>, providing valuable new insights into its epidemiology.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102669"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequencing of rice endophyte Bacillus paralicheniformis NB stem 4: A potential biocontrol agent for the suppression of pearl millet blast disease","authors":"Kushmitha B ,&nbsp;Suhail Ashraf ,&nbsp;Nakkeeran S ,&nbsp;Johnson I ,&nbsp;Saranya N ,&nbsp;Mahendra K ,&nbsp;Mohammad Raish","doi":"10.1016/j.pmpp.2025.102663","DOIUrl":"10.1016/j.pmpp.2025.102663","url":null,"abstract":"<div><div>The supreme role in managing the phytopathogens of cultivable crops had been provided by chemical pesticides. An alternative approach for managing the plant disease with less residual effect on the ecosystem and plant growth promoting attributes are emphasized globally. Hence, biocontrol of plant pathogens using <em>Bacillus licheniformis</em> isolated from Nona Bokra, the salt tolerant rice cultivar was explored for the management of blast infecting pearl millet. Genome annotation of <em>Bacillus licheniformis</em> NB stem 4 was identified as <em>B. paralicheniformis</em> NB stem 4 with accession number SRR21528255. Genome analysis revealed the presence of 958, 374, 583 reads with an average (mean) read length of 2906 bp. There were 11 number of contigs in the assembled genome with total length of 43,13,072 bp with an average G + C content of 45.94 %. The protein coding sequences in the genome were 9984, tRNA regions were 80 with 24 ribosomal RNA (r RNA) genes and 53 repeat regions. The genome annotation of <em>B. paralicheniformis</em> NB stem 4 included 1691 hypothetical proteins and 8293 proteins with functional assignments. Non ribosomal peptide synthetase (NRPS) gene clusters in the genome of NB stem 4 include fengycin, putrebactin/avaroferrin, butirosin A/butirosin B, lichenysin, streptin, equibactin, bacillibactin and alkylpyrone-407/alkylpyrone-393. The <em>in vitro</em> screening for examining the antifungal action of <em>B. paralicheniformis</em> NB stem 4 against <em>Magnaporthe grisea</em> of pearl millet suppressed the mycelial growth <em>M. grisea</em> by producing antimicrobial compounds including piperidinone, 4-quinolinol, 7-hydroxycoumarin, traumatic acid, bioallethrin, actinomycin, clindamycin, lycoxanthin, nizatidine, furanone, triamcinolone acetonide, decatrienoic acid, hexadecanoic acid, eicosadienoic acid, trilinolein, 5-hydroxymethyldihydrofuran-2-one and cyclopentanedione. Thus, <em>B. paralicheniformis</em> NB stem 4 can be explored for the management of <em>M. grisea</em> infecting pearl millet.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102663"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of key differential metabolites in Astragalus membranaceus var. mongholicus against Fusarium oxysporum and assessment of sucrose as putative resistance-related metabolite","authors":"Fen Gao ,&nbsp;Yingzhi Xu ,&nbsp;Li Zhao ,&nbsp;Jianbin Chao ,&nbsp;Ziqing Zhao","doi":"10.1016/j.pmpp.2025.102671","DOIUrl":"10.1016/j.pmpp.2025.102671","url":null,"abstract":"<div><div>Root rot of <em>Astragalus membranaceus</em> var. <em>mongholicus</em> (AMM) is a destructive disease that severely impacts both the production and clinical use of AMM. Cultivating resistant varieties can effectively manage root rot, but the resistance mechanisms and evaluation indicators for AMM against root rot fungi remain unclear. This study aims to investigate the resistance mechanisms of AMM against <em>Fusarium oxysporum</em> (FO), one of the primary pathogens responsible for root rot, using a ¹H nuclear magnetic resonance (NMR)-based untargeted metabolomic approach, and to identify resistance-related (RR) metabolites for guiding future breeding efforts. The results showed that the metabolite profile of AMM was significantly altered by FO infection, with disturbances intensifying over time as the disease progressed. A total of 12 annotated metabolites were identified as differential metabolites (DMs) across three time points. These metabolites exhibited different patterns of abundance changes, with specific changes in sucrose levels suggesting insights into the resistance mechanism. Notably, the sucrose content in a resistant variety-Longqi No. 4 was significantly higher than in a susceptible variety-Longqi No. 1, both before and after inoculation, indicating that sucrose could be a constitutive RR metabolite in AMM. The results obtained from high performance liquid chromatography - quadrupole time-of-flight - mass spectrometry (HPLC-Q/TOF-MS)-based untargeted metabolomic analysis showed that exposure to 8.0 g/L sucrose significantly altered the metabolism of FO strains, and 141 of 177 identified DMs exhibited significantly upregulated abundance. Global natural product social molecular networking (GNPS) analysis suggested that FO strains might produce a wider variety of secondary metabolites under sucrose stress, with certain phospholipid compounds showing significantly higher abundance. These findings enhance our understanding of AMM's resistance mechanisms against FO infection and provide evidence for sucrose as a key RR metabolite in AMM resistance breeding.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102671"},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitinases: Key players in plant defense mechanisms against fungal pathogens","authors":"Gaytri Mahajan,&nbsp;Nivedita Sharma,&nbsp;Manpreet Kaur,&nbsp;Reena Gupta","doi":"10.1016/j.pmpp.2025.102664","DOIUrl":"10.1016/j.pmpp.2025.102664","url":null,"abstract":"<div><div>Throughout history, plant diseases have posed significant challenges to agricultural progress, driven by both abiotic and biotic factors. Abiotic factors include wind, salt damage, freezing, girdling roots and compacted soil, while biotic factors encompass bacteria, nematodes, fungi and viruses. Plants have evolved diverse defense strategies to counter pathogen attacks, one of which involves chitinases, a subset of pathogenesis-related proteins. Chitinases are hydrolytic enzymes that degrade chitin, a high-molecular-weight linear polymer of N-acetyl-D-glucosamine, which is a crucial component of fungal cell walls and septa. These enzymes are produced by a wide range of organisms, including plants, animals, insects, fungi and microorganisms. In plants, chitinases are strongly expressed under pathogenic stress, primarily targeting fungal pathogens by breaking down their cell walls. They also contribute to cell wall remodeling and degradation during growth and defense processes. Numerous studies have demonstrated that the antifungal activity of chitinases is influenced by the chitin concentration and surface microstructure of different fungal species. Research has highlighted their role in protecting plants like mango, cucumber, rye, tomato, grapevine and other plants from various fungal diseases. These findings underscore the critical role of chitinases in plant defense mechanisms, showcasing their importance in mitigating fungal infections and supporting plant health.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102664"},"PeriodicalIF":2.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Pseudomonas aeruginosa and Trichoderma asperellum in promoting plant growth and suppression of Ganoderma boninense disease infestations in oil palm","authors":"Muniroh Ms ,&nbsp;Nusaibah Sa ,&nbsp;Vadamalai G","doi":"10.1016/j.pmpp.2025.102662","DOIUrl":"10.1016/j.pmpp.2025.102662","url":null,"abstract":"<div><div>The most common disease affecting Malaysian palms is basal stem rot (BSR), which is caused by <em>Ganoderma</em> species. Pre-inoculation of oil palm seedlings with <em>Trichoderma asperellum</em> and/or <em>Pseudomonas aeruginosa</em> was conducted to study their effects on the vegetative development and BSR disease suppression. A 32-week nursery trial was conducted on four-month-old oil palm seedlings (D × P) utilizing the dip, place, and drench (DPD) artificial inoculation technique. Disease severity (DS) was determined by root symptoms in conjunction with the influence of vegetative growth. In addition, root samples were collected at eight months after inoculation (MAI) and analyzed using GC-MS to investigate the expression of metabolites generated as a defensive response to <em>G. boninense</em> infection. Using BCAs helped oil palm seedlings grow better than those that weren't treated. The single application of <em>T. asperellum</em> and the combination of <em>P. aeruginosa</em> and <em>T. asperellum</em> had the greatest impact on the growth of the oil palm seedling's plant height (143.0 cm ± 0.02) and (140.45 cm ± 0.02), respectively. Seedlings infected with <em>G. boninense</em> and treated with a mixture of BCAs had the highest top and root weight and the lowest DS (50 %), compared to seedlings infected with <em>G. boninense</em> (positive control) (83.3 %). Furthermore, GC-MS analysis revealed that treatment with BCAs produced plant secondary metabolites that may be involved in antimicrobial and plant defense systems, and as plant growth promoters.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102662"},"PeriodicalIF":2.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and pathogenicity of Colletotrichum species causing anthracnose on pitaya (Hylocereus spp.) in Brazil","authors":"Maria Hilma dos Santos ,&nbsp;Jaqueline Figueredo de Oliveira Costa ,&nbsp;Kevison Romulo da Silva França ,&nbsp;Taciana Ferreira dos Santos ,&nbsp;Mayra Machado de Medeiros Ferro ,&nbsp;Maruzanete Pereira de Melo ,&nbsp;Victor Breno Campelo Lima ,&nbsp;Jorge Luiz Xavier Cunha ,&nbsp;Gaus Silvestre de Andrade Lima ,&nbsp;Iraildes Pereira Assunção","doi":"10.1016/j.pmpp.2025.102657","DOIUrl":"10.1016/j.pmpp.2025.102657","url":null,"abstract":"<div><div>Pitaya (<em>Hylocereus</em> spp.) is a tropical fruit native to southern Mexico, Guatemala, and Costa Rica. Anthracnose symptoms were observed on fruits and cladodes in the pitaya-producing regions of Alagoas and Bahia, Brazil. Fungal isolates from the <em>Colletotrichum</em> genus were obtained from symptomatic tissue. Based on morphological and multigenic analyses (<em>GAPDH</em>, <em>ACT</em>, <em>TUB2</em>, ITS and ApMAT), the isolates were identified as <em>C. chrysophilum</em>, <em>C. siamense</em>, <em>C. theobromicola</em> and <em>C. truncatum</em>. Pathogenicity tests revealed that all isolates caused brown, depressed lesions characteristic of anthracnose on fruit and cladodes. This is the first report of <em>C. chrysophilum</em> and <em>C. theobromicola</em> causing anthracnose on pitaya globally.</div></div>","PeriodicalId":20046,"journal":{"name":"Physiological and Molecular Plant Pathology","volume":"138 ","pages":"Article 102657"},"PeriodicalIF":2.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}